大刺鳅仔、稚鱼的生长及摄食节律

【目的】了解大刺鳅(Mastacembelue armatu)仔、稚鱼的生长特性及摄食节律。【方法】采用生长测量和摄食节律评估方法,在水温25.5～31.0℃条件下,研究人工培育大刺鳅仔、稚鱼的生长及摄食节律。【结果】0～42日龄大刺鳅仔、稚鱼平均全长和体质量的特定增长率分别为6.23%和10.08%;全长(L_t/mm)与日龄(t/d)的回归方程为L_t=0.022 t~2+0.510 t+5.113(R~2=0.991,P<0.01);体质量(m_t/g)与日龄(t/d)的回归方程为M_t=4.464×10~(-4)t~2-0.007 t+0.027(R~2=0.993,P<0.01);体质量(m_t/g)与全长(L_t/mm)的幂函数回归方程为M_t=3.993×10~(-5)L_t~(2.294)(R~2=1.000,P<0.01),幂指数b<3。在实验条件下,大刺鳅仔、稚鱼摄食强度有较明显的昼夜节律,摄食强度高峰期分别为8:00-20:00和8:00-18:00,最高峰均出现在16:00;对卤虫无节幼体和枝角类消化时间分别为12.0～12.5 h和14.0 h。【结论】大刺鳅仔稚鱼的生长属于非均匀生长类型,有较明显的白昼摄食习性。     

黑脊倒刺鲃仔稚鱼摄食的习性与生长

研究了养殖条件下黑脊倒刺仔稚鱼摄食习性与生长,结果表明:仔鱼4日龄开口摄食,全长9.0~9.99mm;仔稚鱼的摄食具有明显的昼夜节律性;仔稚鱼个体间生长速度具有一定的差异性;其体重与摄食量的关系为y=0.064 4X+1.433 1,全长与日龄的关系为L=8.861 4×exp(0.042 9T),体重与日龄的关系为W=3.736×exp(0.151 3T),全长与体重的关系为W=1.7×10-3×L3.531 4。     

线纹尖塘鳢仔幼鱼的生长发育与池塘育苗研究

线纹尖塘鳢的胚后发育大致分为仔鱼、稚鱼、幼鱼和成鱼期。初孵仔鱼仅2.875mm。前期仔鱼混合营养期短,仅2～3d。后期仔鱼为器官发育、奇鳍条基本形成期,历时10d,平均全长从4.31mm增至8.97mm。稚鱼为器官分化完毕,奇偶鳍条分节、鳞被形成期,历时27～29d,长至平均全长27.79mm。早期幼鱼鳞被等发育完善,进入生长时期。仔鱼开口饵料为150～200μm轮虫,其摄饵大小与口宽呈正相关。15mm后稚鱼可驯饵摄食人工混合料。对仔、稚、幼鱼的生长测定结果显示,全长与日龄呈线性关系L=0.5289t-0.7354(r2=0.9720),体重与日龄呈指数函数关系,W=0.8236e0.1267t(r2=0.9611),全长与体重呈幂函数关系,曲线回归方程W=0.0045L3.2527,b=3.2527,属匀速生长型。采用池塘培水,添加外源饵料生物系列,稚鱼全长15mm时开始驯饵。2002-2005年共培育全长2.2～3.4cm早期幼鱼523.7万尾,仔鱼培育成15mm稚鱼的成活率为26.8%,培育成早期幼鱼的成活率为75.7%。     

池塘培育美洲鲥初次性成熟亲本的生长特性

【目的】研究大棚结构式池塘培育的美洲鲥初次性成熟亲本的生长特性。【方法】2016年7月12日至2017年4月20日测量408～690日龄美洲鲥雌雄亲本的体长、体质量、性腺质量,统计分析不同生长阶段的体长、体质量及肥满度。【结果与结论】美洲鲥初次性成熟时,雌雄亲本的体长L与体质量m关系均呈幂函数增长相关,雌雄方程分别为m=0.015 8 L 3.000 2(n=77,R2=0.961 6,P 0.01),m=0. 008 7 L 3.185 0(n=62,R2=0.980 2,P 0.01),幂指数接近于3,均呈等速生长;雌雄亲本肥满度随生长均无显著性变化(P0.05),雌雄鱼肥满度最高值分别出现在617、601日龄;雌鱼个体略大于雄鱼;雌雄亲本生长均可分为稳定生长期和滞长期;雌雄亲本的体长、体质量生长曲线均为有拐点(tr)不对称S型三次函数曲线,雌雄体长方程分别为Lt=-1.176 3×10-6 t 3+1.848 7×10-3 t 2–0.905 733 t+163.714,Lt=-1.288 7×10-6 t 3+2.022 1×10-3 t 2–0.990 363 t+176.202,体质量方程分别为mt=-8.127 66×10-5 t 3+0.131 311 t 2–67.850 8 t+11 535.128,mt=-6.924 07×10-5 t 3+0.111 565 t 2–57.101 9 t+9 580.315(n=9,R20.95,P 0.01),雌雄亲本体长生长拐点分别位于524、523日龄,体质量生长拐点分别位于539、537日龄,生长速度拐点前较快,拐点后明显下降。雌雄亲本分别在430日龄前和421日龄前,体质量生长速度出现负值;在617日龄后,雌雄亲本因性腺发育出现生长滞缓甚至微弱的负生长。     

池养条件下军曹鱼早期发育阶段的生长和摄食

研究了军曹鱼(Rachycentron canadum)早期阶段的生长和摄食特性。结果表明:1~29日龄间,全长与日龄之间呈指数函数相关:y=3.6922e0.0995x(R=0.9913);体重与日龄间呈指数函数相关:y=0.0002e0.276x(R=0.9830);体重与全长之间为幂函数相关:y=5×10-6x2.786(R=0.9957);口径与日龄的关系为直线函数关系:y=0.1034x-0.0044(R=0.9939)。鱼苗早期阶段的生长速度存在显著性差异。军曹鱼早期鱼苗在3~6日龄时主要摄食原生动物、单轮幼虫和轮虫;7~12日龄时主要摄食桡足类无节幼体;13日龄以后主要摄食桡足类成体。仔、稚鱼阶段,军曹鱼的摄食活动呈现明显的昼夜节律,其饱满度指数均在12时前后出现最高峰,日摄食率分别为57.36%和14.78%。     

三角鲤胚后发育研究

三角鲤的胚后发育过程可分为早期仔鱼、晚期仔鱼和稚鱼3个阶段共11个时期。与其他鲤科鱼类比较,三角鲤胚后发育具卵黄囊吸收迅速、鳔迅速发育成形(巡游模式迅速建立)及鳞片的较早出现、长齐和完成胚后发育时间较短等特点;在水温为25.0±0.7℃的条件下,全长5.2±0.2 mm的初孵仔鱼经过24～26 d的发育,全长达20.6±0.3 mm即完成胚后发育;仔鱼、稚鱼的全长随日龄呈线性生长,相关方程式为L=5.8591+0.6254 A(r=0.999 2,P<0.01),每日增长约为0.63 mm。根据三角鲤胚后发育特点讨论了鱼苗生产应注意的问题。     

罗非鱼开口饵料的初步研究

研究了配合饵料、轮虫、蛋黄、轮虫+人工配合饵料(3～12日龄投喂轮虫,13日龄起投喂配合饵料)等4种开口饵料对奥尼罗非鱼仔鱼生长、发育和存活影响。结果表明,1)投喂不同饵料对奥尼罗非鱼仔鱼生长具有显著影响(P<0.05),轮虫+配合饵料组生长较快,绝对增重率和特定增重率分别为0.011g/d和17.18%d-1;蛋黄组生长最慢,绝对增重率和特定增重率与其他各组差异显著(P<0.05)。2)轮虫+配合饵料与轮虫组的仔鱼成活率较高,分别为90.3%和89.7%,2组差异不显著(P>0.05),且仔鱼发育较好,大部分个体进入稚鱼阶段;蛋黄组成活率最低,只有40.7%,个体发育较为缓慢,与其他各组差异显著(P<0.05)。可见,投喂鲜活的天然饵料(轮虫)+配合饵料是罗非鱼苗种培育的最佳开口饵料。     

温度对奥尼罗非鱼仔稚鱼生长、饲料利用和消化酶活力的影响

研究了温度22、25、28、31和34℃下奥尼罗非鱼(Oreochromis niloticus×O.aureus)仔稚鱼生长、饲料利用和消化酶活力。结果表明:1)不同温度对奥尼罗非鱼仔稚鱼生长有显著影响(P<0.05),温度为31℃时,仔稚鱼生长速度和绝对增重率最高,分别为0.08cm/d和0.035g/d;温度为22℃时最低,仅0.06cm/d和0.020g/d。2)饲料系数在28℃时最低,仅1.90;22和34℃时较高,达2.14和2.17,各组差异显著(P<0.05)。3)在仔稚鱼发育和存活率上,当温度在22～34℃时,温度越高,仔稚鱼发育越好;各组仔稚鱼的存活率介于93.7%～94.7%,差异不显著(P>0.05)。4)在消化酶活力上,胃蛋白酶、胰蛋白酶和淀粉酶活力随温度升高而升高,均在34℃时活力最高;脂肪酶活力在31℃时达到最高,22℃时活力最低。     

盐度对菊黄东方鲀受精卵孵化和仔鱼生长的影响

比较在0、5、10、15、20、25、30(自然海水)、35、40、45、50等11个盐度梯度下,菊黄东方鲀(Takiugu flavidus)的受精卵发育和仔鱼生长情况,测定仔鱼的不投饵存活系数(SAI).结果表明:1)菊黄东方鲀受精卵在盐度0~50范围内均可孵化,最适盐度20~25.盐度(x)与孵化时间(y)呈正相关关系,表达式为:y=0.345 x+141.1,R2=0.956.2)实验盐度范围内,菊黄东方鲀仔鱼SAI值为1.55~30.10.3)在盐度5~45条件下,仔鱼皆可存活,存活率为3%~27%.其中,在10~30盐度下存活率较高,为20%~27%.经15 d的培育,仔鱼平均全长为6.28~7.34 mm.低盐度对菊黄东方鲀仔鱼生长更有利,在15~20盐度条件下,仔鱼生长较好,最终平均全长为7.30~7.34 mm.4)根据SAI值和生长状况,仔鱼生长和存活的适宜生长盐度为10~30,最适盐度范围为15~20.     

企鹅珍珠贝人工苗生长的初步观察

为了解企鹅珍珠贝生长规律，观察了壳高2．5～5．0mm的出池幼苗，按月测量其生长参数和成活率，以及环境因子。结果表明，企鹅珍珠贝生长最快的月份为7～11月和次年4～6月，壳长，壳高和壳宽月均增加量分别为3．8～13．0mm、4．7～11．2mm、2．3～3．8mm，月成活率97．8％～98．6％。企鹅珍珠贝生长最慢月份是11月至次年3月。     

Artificial culture of amphioxus (Branchiostoma belcheri tsingtauense)

Mature amphioxus (Branchiostoma belcheri tsingtauense) collected by trawl in June, 1994 from Qingdao Shazikou sea area bottom sands, were cultured under controlled indoor conditions, where they spawned and their eggs were fertilized and hatched into larvae which gradually settled at the bottom and dug into the sands after 40-50 days culture in a water trough outdoors. The total survival rate of the larvae(LSR)was 5.5% before they went into the sands, 1.9% in 4 months, 0.7% in 10 months. The survival rate of the young fish which had dug into the sands (YSR) was 35.6% in 4 months, and 12.6% in 10 months. The amphioxus number tended to be constant from the 11th month on. In the first five months after incubation, the amphioxus body length increased by an average of about 1.5 mm a month, and about 0.4 mm a month from Dec. to May of next year. The maximum length after ten months was 24 mm; the average was about 11 mm. After two years culture in the water trough, the maximum length could reach 34-35 mm, when the amphioxus gonads began to develop.     

Embryonic and larval development in barfin flounder Verasper moseri (Jordan and Gilbert)

Broodstock of Verasper moseri (Jordan and Gilbert) aged 3–4 years old were selected, and reinforced cultivation was conducted to promote maturation under controlled water temperature and photoperiod conditions. Fertilized eggs were obtained by artificial fertilization, and the development of embryos, larvae and juveniles was observed continuously. The results showed that the fertilized eggs of V. moseri were spherical, with transparent yolk and homogeneous bioplasm, and had no oil globule inside. The average diameter of the eggs was 1.77±0.02 mm. The eggs of V. moseri were buoyant in water with salinity above 35. The cleavage type was typical discoidal. Young pigment cells appeared when olfactory plates began to form. Hatching occurred at 187 h after fertilization at a water temperature of 8.5°C. The newly hatched larvae, floating on the water surface, were transparent with an average total length of 4.69±0.15 mm. During the cultivation period, when the water temperature was raised from 9 to 14.5°C, 4-day old larvae showed more melanophores on the body surface, making the larvae gray in color. The pectoral fins began to develop, which enabled the larvae to swim horizontally and in a lively manner. On days 7–8, the digestive duct formed. The yolk sac was small and black. The yolk sac was absorbed on day 11. Larvae took food actively, and body length and body height clearly increased. The rudiments of dorsal and anal fin pterygiophores were discernible and caudal fin ray elements formed on day 19. On day 24, the larval notochord flexed upwards, and the rays of unpaired fins began to differentiate. Pigment cells converged on the dorsal and anal fin rays, and the mastoid teeth on the mandible appeared. On day 29, the left eyes of juveniles began to move upwards. Depigmentation began in some juveniles and they became sandy brown in color on day 37. Most juveniles began to settle on the bottom of the tank. The left eyes of juveniles migrated completely to the right side on day 50, when the average body length attained 2.5±0.18 cm, and juveniles accomplished metamorphosis to young. The embryonic and larval characters of several flounder species are compared.     

长三角地区GNSS大气水汽转换系数模型精化研究

根据长三角地区7个探空站基于积分法计算的2016年大气水汽转换系数（K值）,利用多元线性拟合分别构建不顾及高程的Emardson-I精化模型和顾及高程的Emardson-H精化模型,并用2017年的K值验证两种模型的精度。实验结果表明,Emardson-H预报模型的MAE和RMS分别为0.001 297和0.001 616,略优于Emardson-I预报模型的0.001 303和0.001 620;基于两种新模型的GNSS-PWV反演精度相当,其MAE和RMS均优于0.6 mm。因此,Emardson-I模型以其无需实测气象参数和无需顾及高程在长三角地区的地基GNSS气象学实时应用中具有更好的效率优势。     

Exercise in downscaling on sea surface temperature along Chinese coast

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Ontogenetic development in the morphology and behavior of loach （Misgurnus anguillicaudatus） during early life stages

Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide insight into ways to improve larviculture techniques, we describe the morphological characteristics and behavior of loach during the larval and early juvenile stages. Yolksae larvae ranged from 2.8 to 4.0 mm body length （BL） between days 0 to 4; preflexion larvae ranged from 3.6 to 5.5 mm BL between days 4 to 6; flexion larvae ranged from 4.8 to 8.1 mm BL between days 5 and 14; and post-flexion larvae ranged from 7. l to 15.7 mm BL between days 11 to 27; the minimum length and age of juveniles was 14.1 mm BL and 23 d, respectively. Loach are demersal from hatch through to the early juvenile stages. A suite of morphological characteristics （e.g., external gill filament and ventral mouth opening） and behavioral traits have developed to adapt to demersal living. We observed positive allometric growth in eye diameter, head length, head height, and pectoral fin length during the early larval stages, reflecting the priorities in the development of the organs essential for survival. Our results provide a basis for developing techniques to improve the survival of larval and juvenile loach during mass production.     

利用正向建模方法反演三峡库区水储量变化

针对GRACE数据后处理过程中滤波导致的信号衰减和泄漏,利用模拟数据研究正向建模改正,改正的量级约为1 cm,在利用GRACE反演陆地水储量变化时这一影响因素不可忽略。基于2002-07~2014-12的GRACE Level-2数据反演三峡库区的水储量变化,结果表明,正向建模改正前后GRACE年周期项振幅分别为7.0±2.4 cm和7.2±2.7 cm,改正后信号泄漏有所减小;GRACE和水文模式数据均表现出明显的季节性特征,但GRACE受多种因素的综合影响,其周年项振幅略大于水文模式;水库3次蓄水引起的等效水高变化分别为60.2 mm、28.3 mm、20.5 mm,与利用水位观测估计得到的库区容量变化具有较好的一致性;分析2003~2009年正向建模改正前后GRACE反演得到的三峡地区等效水高变化速度的空间分布发现,改正后库区蓄水信号明显收敛。     

基于GPT3模型的ZTD及PWV反演精度分析

使用亚洲区域18个IGS测站和中国区域内16个探空站2016~2018年的数据,研究GPT3模型反演天顶对流层延迟（ZTD）和大气可降水量（PWV）的精度,并与其他GPT系列模型进行对比。结果表明,GPT3-1模型估计的ZTD的bias均值和最大值均最小,分别为1.34 mm和14.06 mm;GPT3模型整体精度略优于GPT2w模型,优于GPT2模型。探空站处GPT3模型反演的PWV的bias和RMSE均表现出较强的季节性特征;由GPT3模型反演的PWV的月均值可知,GPT3-1模型比GPT3-5模型具有更高的精度和稳定性。     

盐度对珠母贝幼虫和稚贝存活和生长的影响

为了解珠母贝早期发育阶段最适存活和生长条件,于2013年5—6月在珍珠贝养殖场进行不同海水盐度对珠母贝直线铰合期幼虫、壳顶期幼虫和稚贝生长及存活的影响实验。结果表明：珠母贝直线铰合期幼虫存活适宜盐度为2285—3886,最适盐度为2719—3502,壳长增长的适宜盐度为2043—4123,最适盐度为3110;壳顶期幼虫存活适宜盐度为2310-3914,最适盐度为2719—3502,壳长增长的适宜盐度为2029—4009,最适盐度为3110·3502;稚贝存活适宜盐度为2064—3910,最适盐度为2719—3502,壳长增长的适宜盐度为1938—4130,最适盐度为2719—3110。     

吉富罗非鱼最适生长水温研究

为研究不同养殖水温对吉富罗非鱼摄食和生长的影响,了解吉富罗非鱼的最适生长水温,实验设计28℃、30℃、32℃、34℃4个不同温度梯度,周期45 d。结果表明:不同水温环境下吉富罗非鱼表现出不同的生长特性,养殖在30℃水体中的罗非鱼生长速度明显高于其他温度组;水温对吉富罗非鱼的特定生长率、绝对增重率、饲料转化率、摄食率有显著影响(P<0.05),之间的相关关系可以用二次回归曲线来描述,特定生长率(SGR)与水温的关系式为rSGR=-19.255+1.3794T-0.0235T2,摄食率(FR)与水温的关系式为rFR=-22.175+1.5901T-0.026 6 T2,饲料转化率(FCR)与水温的关系式为rFCR=34.041-2.2256T+0.0382 T2;吉富罗非鱼的最适生长水温为29.3℃,最大摄食率的水温为29.9℃,最高食物转化率水温为29.1℃。     

Otolith development in larval and juvenile Schizothorax davidi: ontogeny and growth increment characteristics

Laboratory-reared Schizothorax davidi larvae and juveniles were examined to assess the formation and characteristics of David’s schizothoracin otoliths.Otolith development was observed and their formation period was verifi ed by monitoring larvae and juveniles of knownage.The results revealed that lapilli and sagittae developed before hatching,and the first otolith increment was identified at 2 days post hatching in both.The shape of lapilli was relatively stable during development compared with that of sagittae;however,growth of four sagittae and lapilli areas was consistent,but the posterior area grew faster than the anterior area and the ventral surface grew faster than the dorsal surface.Similarly,the sum length of the radius of the anterior and posterior areas on sagittae and lapilli were linearly and binomially related to total fish length,respectively.Moreover,daily deposition rates were validated by monitoring knownage larvae and juveniles.The increase in lapilli width was 1.88±0.080 0μm at the ninth increment,which reached a maximum and the decreased gradually toward the otolith edge,whereas that of sagittae increased more slowly.These results illustrate the developmental biology of S.davidi,which will aid in population conservation and fish stock management.