A survey of the occurrence of Bacillus anthracis in North American soils over two long-range transects and within post-Katrina New Orleans

Soil samples were collected along a north–south transect extending from Manitoba, Canada, to the US–Mexico border near El Paso, Texas in 2004 (104 samples), a group of sites within New Orleans, Louisiana following Hurricane Katrina in 2005 (19 samples), and a Gulf Coast transect extending from Sulphur, Louisiana, to DeFuniak Springs, Florida, in 2007 (38 samples). Samples were collected from the top 40 cm of soil and were screened for the presence of total Bacillus species and Bacillus anthracis (anthrax), specifically using multiplex-polymerase chain reaction (PCR). Using an assay with a sensitivity of 170 equivalent colony-forming units (CFU) g⁻¹ field moist soil, the prevalence rate of Bacillus sp./B. anthracis in the north–south transect and the 2005 New Orleans post-Katrina sample set were 20/5% and 26/26%, respectively. Prevalence in the 2007 Gulf Coast sample set using an assay with a sensitivity of 4 CFU g⁻¹ of soil was 63/0%. Individual transect-set data indicate a positive relation between occurrences of species and soil moisture or soil constituents (i.e., Zn and Cu content). The 2005 New Orleans post-Katrina data indicated that B. anthracis is readily detectable in Gulf Coast soils following flood events. The data also indicated that occurrence, as it relates to soil chemistry, may be confounded by flood-induced dissemination of germinated cells and the mixing of soil constituents for short temporal periods following an event.     

Exploring the factors affecting urban ecological risk: A case from an Indian mega metropolitan region

Assessment of ecological risk (ER) is a key approach to adapting and mitigating ecological deterioration in cities of developing countries. In developing countries, the ecological landscapes such as vegetation cover, water bodies, and wetlands are highly vulnerable due to rapid urban expansion. Therefore, urban ER (UER) assessment and its drivers are crucial to guide ecological protection as well as restoration. This study aims to explore the spatiotemporal pattern of UER and the impact of urban spatial form on UER in the Kolkata Megacity Region (KMR), India. This study developed a UER index and used spatial regression models across the urban centres. The ER has been assessed at city scale as well as grid-scale (2 km × 2 km and 5 km × 5 km) from 2000 to 2020. The results showed that ER has substantially increased over the last 20 years. The urban centres with very high and high ER substantially increased, i.e. from 21.95% in 2000 to 31.70% in 2020. Kolkata and its surrounding urban centres were mostly characterized by very high and high ER. ER was influenced by spatial variables (such as land use and landscapes pattern). However, remote sensing parameters were weakly related to ER. The spatial lag model (SLM) (R² = 0.8686) was found to be better fit model than spatial error model (SEM) (R² = 0.8661) and ordinary linear regression model (OLS) (R² = 0.8641). Thus, the findings of the study can improve research and a comprehensive framework for urban ecological resources and provide a scientific basis for urban ecosystem planning and restoration. In addition to this, it will guarantee the sustainable utilization of urban ecosystems.     

Sporormiella as a proxy for non-mammalian herbivores in island ecosystems

Spores of the dung-fungi Sporormiella are routinely used as a proxy for past megaherbivore biomass and to pinpoint the timing of extinctions. Further ecological insight can also be gained into the impacts that followed initial human arrival in a region through correlation of spore abundance with other proxies (e.g. pollen, charcoal). Currently, the use of Sporormiella as a palaeoecological proxy has been restricted to landmasses where large-herbivore guilds are dominated by mammals. Here, we use New Zealand as a case study to show that the method can also be applied effectively to islands dominated by large avian herbivores. We examine 44 dung samples from 7 localities to show that Sporormiella spores were widely distributed in the dung of endemic avian herbivores (South Island takahe (Porphyrio hochstetteri), kakapo (Strigops habroptilus), and several species of extinct moa, identified by ancient DNA analysis). In addition, we show that Sporormiella spores in a forest soil core from the Murchison Mountains, South Island, accurately trace the post-settlement decline of native avian herbivores, and combined with high-resolution radiocarbon dating reveal severely reduced local herbivore populations by the late 17th Century AD. The spores also trace the subsequent spread of Red deer (Cervus elaphus) introduced to the area in the early 20th Century AD. Our results suggest Sporormiella spores may provide a useful new tool for examining extinctions on numerous islands where terrestrial herbivore guilds were dominated by birds or reptiles. Our findings also highlight the need to consider entire herbivore communities (including birds and reptiles) when examining Late Pleistocene continental Sporormiella records, where the focus has often been tracing the decline of populations of large mammals.     

The spatial characteristics and pollution levels of metals in urban street dust of Beijing,China

The components and concentrations of metals in street dust are indictors of environmental pollution. To explore the pollution levels of Cd, Cr, Cu, Mn, Ni and Pb in street dust and their spatial distribution characteristics, 220 dust samples were collected in a grid pattern from urban street surfaces in Beijing. Multivariate statistics and spatial analyses were adopted to investigate the associations between metals and to identify their pollution patterns. In comparison with the soil background values, elevated metal concentrations were found, except those for Mn and Ni. The results of the geo-accumulation index (I_geo) and the potential ecological risk index (Erⁱ) of the metals revealed the following orders: Cd > Cu > Cr > Pb > Ni > Mn and Cd > Cu > Pb > Cr > Ni. Levels of I_geo ranging from 0 to 5 were found and about 80% of the samples were below the moderately polluted level. The Erⁱ values of single elements were within the low ecological risk level in most sampling sites. Most of the metals in the street dust of Beijing were statistically significantly correlated. It is hard to clearly identify the sources of each metal in the street dust since local environments are very complex. Cadmium, Cu, Cr, Mn and Pb showed medium spatial autocorrelations within the sampling region. Similar spatial distribution patterns were observed for Cu, Cr and Pb, and these metals had relatively high spatial variabilities and were enriched in the center of the city with several peaks scattered in the suburbs. Metal pollution anomalies were identified by using cluster and outlier analyses. Locations identified as clusters with high values indicated non-point source pollution, while locations identified as outliers with high values indicated point source pollution. Traffic, construction, and other human activities influenced these high values. In addition, the locations identified as outliers with low values in urban areas might benefit from less transportation and better management.     

Bacillus cereus,gold and associated elements in soil and other regolith samples from Tomakin Park Gold Mine in southeastern New South Wales,Australia

Efficient exploration for new Au deposits is increasingly important as existing deposits become depleted. This is particularly relevant in Australia, where exploration can be difficult because of a thick regolith cover. New and effective methods of exploration need to be developed, and possibilities lie in geomicrobiological methods. For instance, Bacillus cereus, a common soil bacterium, has been shown to act as a biogeochemical indicator for concealed mineralisations, including vein-type Au deposits. We report the results of the first Australian case study of the association of B. cereus and Au at the Tomakin Park Gold Mine in southeastern New South Wales. Soil samples from the Ah horizon were analysed for B. cereus spores and 56 major and trace elements. The results show enrichment of Au, As, B. cereus spores and, to a lesser extent, Sb, Bi and Pb over the top of the Au deposit. Gold concentrations over the mineralisation range from 100 ppb to 1.1 ppm compared to a background of 2 ppb and As concentrations are enriched to 100 ppm from a background of 5 ppm. B. cereus spore counts were up to 10 times higher in soils with elevated concentrations of Au. Factor analysis indicates four main associations: TiO₂+lanthanides+actinides; CaO+MgO+Cs+Be+Ba(+Ga+Pb+Rb); B. cereus+Au+As+Sb+Bi(+Pb); Fe₂O₃+MnO+Co+Ni+Cu+Mo. Selective sequential leaching was used to study the fractionation of Au and As in soils, other regolith materials and Au-bearing vein quartz to infer their mobility and bioavailability. In unweathered quartz vein material, the majority of the Au was extracted only in the strongest, final step, with aqua regia. However, in soils from the Ah horizon, 50% of the Au was present in the water-, ammonium acetate- and sodium pyrophosphate- and hydroxylamine hydrochloride-extractable fractions. In contrast, As displays little change in fractionation with an increasing degree of weathering, and is predominantly associated with the operationally defined Mn- and Fe-oxides and oxyhydroxides. These results indicate that: (i) Au is mobilised during the weathering of the host rock; (ii) Au is bioavailable in these soils; and (iii) the increase in B. cereus spores is likely to be linked to elevated concentrations of bioavailable Au in these soils. The results indicate also that an effective biogeochemical exploration technique may be developed, where B. cereus spore counts are measured in the field and used as a pre-screening method to target areas useful for further sampling and complete geochemical analysis.     

Late Quaternary vegetation reconstruction from the Eastern Arc Mountains, Tanzania

Pollen, spore, macrofossil and stable isotope (C and N) analyses from a 266-cm sediment core collected from a swamp on the Eastern Arc Mountains, Tanzania, are used to reconstruct vegetation and environmental history. An estimated time scale based on five ¹⁴C ages records approximately 38,000 yr. This palaeorecord is the first from this biodiversity hotspot and importantly extends through the last glacial maximum (LGM). The altitudinal transition from montane to upper montane forest shifted from 1700-1800 m (38,000 ¹⁴C yr BP) to 1800-1900 m (35,000-29,000 ¹⁴C yr BP). From 29,000 to 10,000 ¹⁴C yr BP, it shifted from 1850-1950 m across the LGM to 1750-1800 m (during 10,000-3500 ¹⁴C yr BP), and to present-day elevations at 2000 m during the last 3500 ¹⁴C yr BP. The relative ecosystem stability across the LGM may be explained by the Indian Ocean's influence in maintaining continuous moist forest cover during a period of East African regional climate aridity. During the late Holocene, presence of abundant coprophilous fungi and algal blooms demonstrates increasing human impact. Neurospora spores indicate frequent fires, coinciding with clear signals of decline in Podocarpus and Psychotria trees that possibly represent selective logging.     

Characteristics of Bacillus cereus Spore Counts in Soils with Underlying Gold Mineralization in Northwestern Sichuan, China. Results from a Pilot Study

A pilot survey on a microbial mineral exploration method based on soil Bacillus cereus spore counts was carried out across three different gold mining regions, which vary in soil type, climate condition and geological setting in northwestern Sichuan, China. B-horizon soils from these sites were analyzed for B. cereus spores, Au, Ag, Cu, Pb and Zn. The results show that the nmnbers of B. cereus spores generally increased in soils overlying gold mineralization. Specifically, elevated spore counts were found in samples slightly offset from the outcrops of orebodies, whereas soils directly above the outcrops of orebodies usually contained low spore counts. However, the background counts of B. cereus spores varied from place to place and were complicated by environmental and pedological factors, but the rela-tive ratios of spore counts still were indicative of the underlying gold mineralization.     

Contribution des écosystèmes continentaux à la séquestration du carbone

This article focuses on the contribution of natural ecosystems (forests, grasslands) and agrosystems to carbon sequestration either in biomass or in soil. Carbon stocks are important (650 Gt in biomass, 1500 to 2000 Gt in soils as compared with 750 for atmospheric CO₂), and also fluxes that led to CO₂ emissions in the past (due to deforestation or cultivation) and which now turn to carbon sequestration (2 Gt_C/year). This article shows great spatial variations in stocks and fluxes and great measurement difficulties, especially for stock variations. Anthropic actions such as reforestation (mainly in the North), changes in land use or in crop management, can increase carbon sequestration in biomass or soil, with a residence time of several decades, which is not insignificant with respect to the Kyoto protocol and which also has other environmental benefits. To cite this article: M. Robert, B. Saugier, C. R. Geoscience 335 (2003).     

Spatial variability assessment of soil nutrients in an intense agricultural area,a case study of Rugao County in Yangtze River Delta Region,China

Topsoil samples (0–20 cm) (n = 237) were collected from Rugao County, China. Geostatistical variogram analysis, sequential Gaussian simulation (SGS), and principal component (PC) analysis were applied to assess spatial variability of soil nutrients, identify the possible areas of nutrient deficiency, and explore spatial scale of variability of soil nutrients in the county. High variability of soil nutrient such as soil organic matter (SOM), total nitrogen (TN), available P, K, Fe, Mn, Cu, Zn, and B concentrations were observed. Soil nutrient properties displayed significant differences in their spatial structures, with available Cu having strong spatial dependence, SOM and available P having weak spatial dependence, and other nutrient properties having moderate spatial dependence. The soil nutrient deficiency, defined here as measured nutrient concentrations which do not meet the advisory threshold values specific to the county for dominant crops, namely rice, wheat, and rape seeds, was observed in available K and Zn, and the deficient areas covered 38 and 11%, respectively. The first three PCs of the nine soil nutrient properties explained 62.40% of the total variance. TN and SOM with higher loadings on PC1 are closely related to soil texture derived from different parent materials. The PC2 combined intermediate response variables such as available Zn and P that are likely to be controlled by land use and soil pH. Available B has the highest loading on PC3 and its variability of concentrations may be primarily ascribed to localized anthropogenic influence. The amelioration of soil physical properties (i.e. soil texture) and soil pH may improve the availability of soil nutrients and the sustainability of the agricultural system of Rugao County.     

Ultimate lateral bearing capacity of tetrapod jacket foundation in clay

The tetrapod jacket foundations that are always used to support offshore wind turbines have been investigated primarily in laboratory experiments. In this study, the ultimate lateral soil resistance on this type of foundation was investigated using the finite element method and the analytical upper bound plasticity method. The numerical results show good agreement with the theoretical upper bound solutions under the same pile spacings (S) and soil-pile adhesion factors (α). Three distinct failure mechanisms (mechanisms A, B and C) were established in terms of different pile spacings. The ultimate lateral pressure was subsequently determined using numerical analyses with consideration of the loading direction. The most critical loading direction angles (θ) vary with the soil-pile adhesion factors, and are θ = 0 for α = 1 and θ = π/4 for α = 0. Selected empirical equations were proposed to predict the ultimate lateral bearing capacity for engineering practice, considering the pile spacing, soil-pile adhesion and loading direction.     

Assessment of effects of heavy metals combined pollution on soil enzyme activities and microbial community structure: modified ecological dose–response model and PCR-RAPD

A laboratory study was conducted to evaluate the response of soil enzyme activities (namely dehydrogenase, phosphatase and urease) to different levels of trace element pollution in soil representative area. The improved ecological dose model and random-amplified polymorphic DNA (RAPD) were used to assess soil health. The 50% ecological dose (ED₅₀) values modified by toxicant coefficient were calculated from the best-fit model, and determination values from the regression analysis for the three enzyme activities were studied after the incubation periods. The results showed that the elevated heavy metal concentration negatively affects the total population size of bacteria and actinomycetes and enzymatic activity; dehydrogenase (ED₅₀ = 777) was the most sensitive soil enzyme, whereas urease activity (ED₅₀ = 2,857) showed the lowest inhibition; combined pollution or elevated toxicant level would increase disappearing RAPD bands, and the number of denoting polymorphic bands was greater in combined polluted soils. All three mathematical modified models satisfactorily described the inhibition of soil enzyme activities caused by Cd and Pb, by giving the best fit.     

Palynostratigraphy of the Chapada Group and its significance in the Devonian stratigraphy of the Paraná Basin,south Brazil

The Alto Garças Sub-basin in the northern part of the Paraná Basin evolved differently from the Apucarana Sub-basin in the south. The marine environment was shallower in the Alto Garças Sub-basin, which contains proportionately more silty and arenaceous rocks. The formations and members defined in the Apucarana Sub-basin are therefore difficult to apply in the Alto Garças Sub-basin, where the Chapada Group (units 1–4) is more applicable. An integrated miospore and chitinozoan biozonation of the Chapada Group facilitates direct correlation between the Chapada Group’s units and the classical formations of the Paraná Basin as defined in the Apucarana Sub-basin. The Furnas Formation and Chapada unit 1 constitute the same lithostratigraphic unit. Beds with rhyniophytes in the uppermost part of the Furnas Formation contain palynomorphs representative of the Si phylozone within the MN spore Zone (late Lochkovian), and the rhyniophyte beds occupy the same stratigraphic interval within Chapada unit 1 (the Lochkovian of the Paraná Basin lacks chitinozoans). The lower part of Chapada unit 2 contains spores of the PoW Su spore Zone and chitinozoans of the Ramochitina magnifica and Ancyrochitina pachycerata zones, together indicating a late Pragian–early Emsian age-span. The upper part of Chapada unit 2 corresponds to the GS (AP) and Per (AD pre-Lem) spore Zones, and chitinozoans of the Ancyrochitina parisi, the informal Ancyrochitina varispinosa and Alpenachitina eisenacki chitinozoan zones, thus suggestive of a late Emsian – earliest Givetian age-span. Unit 3 is a proximal and lateral facies equivalent of the upper part of unit 2. The lower part of unit 4 includes spores typical of the early Givetian Lli (AD Lem) spore Zone and chitinozoans of the Ramochitina stiphrospinata chitinozoan Zone; the uppermost (early late Frasnian) part contains spores of the lower BMu (IV) spore Zone and chitinozoans of the Lagenochitina avelinoi chitinozoan Zone. The sandstones of unit 3 were inundated during the earliest Givetian, and the resultant flooding surface marks the base of unit 4 basin-wide. Clearly, the two sub-basins were distinct depositional centers during the Devonian.     

Spatial heterogeneous response of land use and landscape functions to ecological restoration: the case of the Chinese loess hilly region

Ecological restorations over time may have profound effects on ecological and socio-economic systems. However, land-use changes and landscape functions that accompany ecological restorations can have spatial differentiations due to varied biophysical and socio-economic contexts. Therefore, these spatial differentiations caused by ecological restoration must be understood for better planning and management of restoration activities. The Baota District, with 576 villages in the center of the Chinese Loess Plateau, was selected as the study area because of its dramatic transition from cropland to grassland and shrubland from 1990 to 2010. Using the ArcGIS software and a k-means clustering analysis, an approach to identify types of land-use change patterns (TLCPs) at the village level was developed, and four TLCPs were delineated. The analysis indicated a general pattern of cropland decline by 21.6 %, but revealed significant spatial variations between villages in different TLCPs. Vegetation cover and soil retention, which are key proxies for landscape functions, increased by 22.70 and 108 %, respectively, from 2000 to 2010 with significant spatial heterogeneity. The Universal Soil Loss Equation was employed for the assessment of soil retention. The analysis of landscape metrics revealed a major trend of fragmentation and regularity on the county and village scale; however, spatial variations remained. Physical attributes were used to characterize different TLCPs, and notable differences were found. The spatial heterogeneous change in land use and landscape functions on the village scale may be useful for land use and ecological restoration management policy makers.     

Porosity change model for watered super absorbent polymer-treated soil

Drought is a great concern in agricultural production, because it restricts normal plant growth, brings about enormous economic loss and deteriorates ecological environment. Proper use of super absorbent polymers (SAP) is helpful in the agricultural and horticultural industry in arid and semi-arid areas, because SAP can ease the burden of water shortage. Because porosity is one of the most important soil physical properties, it is a priority to study SAP to quantitatively express the swelling of watered SAP-treated soil. This study was aimed to evaluate the bulk density curve of watered SAP-treated soil and to construct and test the model for porosity change of watered SAP-treated soil. The results showed that the application of SAP can reduce soil bulk density, improve soil permeability and cause soil swelling. In addition, using three factors, i.e., water content, change in swelling ratio and SAP application rate, the paper constructed a model for porosity change of watered SAP-treated soil, which is {ln[(P _m − P)(P _m − P ₀)⁻¹]} βP ₀ θ = −η ₀ cθ − η ₀ a. This is a generic model. Two soil samples, namely, loam and sandy loam, were used to calculate the parameters and test the model. The results of the model were satisfying, thus this model is reliable.     

Analysis of coprolites from the extinct mountain goat Myotragus balearicus

Humans colonized the Balearic Islands 5–4 ka ago. They arrived in a uniquely adapted ecosystem with the Balearic mountain goat Myotragus balearicus (Bovidae, Antilopinae, Caprini) as the only large mammal. This mammal went extinct rapidly after human arrival. Several hypotheses have been proposed to explain the extinction of M. balearicus. For the present study ancient DNA analysis (Sanger sequencing, Roche-454, Ion Torrent), and pollen and macrofossil analyses were performed on preserved coprolites from M. balearicus, providing information on its diet and paleo-environment. The information retrieved shows that M. balearicus was heavily dependent on the Balearic box species Buxus balearica during at least part of the year, and that it was most probably a browser. Hindcast ecological niche modelling of B. balearica shows that local distribution of this plant species was affected by climate changes. This suggests that the extinction of M. balearicus can be related to the decline and regional extinction of a plant species that formed a major component of its diet. The vegetation change is thought to be caused by increased aridity occurring throughout the Mediterranean. Previous hypotheses relating the extinction of M. balearicus directly to the arrival of humans on the islands must therefore be adjusted.     

Geochemical contamination in the mine affected soil of Raniganj Coalfield——A river basin scale assessment

The study aimed to assess the heavy metals(K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, As, Pb, Sr, Zr) contamination in the soil of mine affected Singaran river basin and to analyse spatial variation in the contamination level considering 32 soil samples. Elemental analysis of soil samples has been performed through Energy Dispersive X-ray Analysis(EDX) to quantify the elemental concentration(mg kgà1). Heavy metal concentrations have been assessed through geo-accumulation index(Igeo) and enrichment factor(EF).Indices showed soils have moderate accumulation of most of the metals with moderate enrichment of Sr,Zr, Zn, Cu and Ni. Soil contamination level assessment has been carried out using indices like Contamination Factor(CF), degree of contamination(C_(deg)), modified degree of contamination(m C_(deg)) and Pollution Load Index(PLI). CF shows moderate to considerable contamination by Sr, Zr, Ca, Cu, Mn, Zn and Ni. Mean indices values(m C_(deg)and PLI for the entire basin are 3.38 and 2.23 respectively) show low to moderate level of soil contamination. These indices result have been mapped and analysed in GIS platform to get spatial variation of pollution level. Opencast mines dominate middle catchment area and so is comparatively contaminated. Sample sites 11, 18 and 25 evidenced high values of all indices of pollution load. From the ecological standpoint Ecological Risk Factor(Er) and Potential Ecological Risk Index(RI) have been estimated to assess regional threat to native soil environment and it shows low ecological risk potential. Analysis shows that mine dominated soil of the entire Singaran basin is less contaminated in all respect but tends to the moderate contamination level at the mid-catchment area,especially by Sr, Zr, Zn, Cu and Ni.     

Spatial variability of heavy metals in soils across a valley plain in Southeastern China

An investigation was carried out to survey the magnitude and spatial distribution of heavy metals, as well as their relation with soil series, in a valley plain in Southeastern China. Soil was sampled at 159 sites by combining a squared grid and nested sampling strategies along the transect perpendicular to the Qujiang River in Zhejiang Province, China. Total concentrations of six metals, namely Cu, Fe, Mn, Ni, Pb and Zn, were measured. Classical statistics and geostatistics were used to quantify their spatial characteristics. There was a considerable variation in many of these parameters. The total concentrations ranged from 6.8 to 29.3 mg kg⁻¹ for Cu, 6,784 to 18,678 mg kg⁻¹ for Fe, 94 to 385 mg kg⁻¹ for Mn, 6.1 to 20.3 mg kg⁻¹ for Ni, 25.0 to 49.5 mg kg⁻¹ for Pb, and 12 to 160 mg kg⁻¹ for Zn. Pearson correlation coefficients among total metal concentrations and selected soil properties showed a number of strong associations. By virtue of analysis of variance, a predominant influence of soil series on the spatial variability of metal concentrations was observed. All metals were spatially correlated. The semivariograms of Cu, Fe, Mn, Ni and Zn were dominated by short range correlation (600 or 700 m), and that of Pb by long range (1200 m). Block kriging maps of total metal concentrations and soil properties showed strip distributions, perpendicular to the river, in the manner similar to the soil series. Principal component analysis was run to identify common distribution patterns of heavy metals and soil properties. These results illustrate that soil series information of valley plain may be useful for developing management zones for site-specific agriculture.     

Notes on the divergence time of the fern genus Asplenium from fossil and molecular evidence

The extant fern genus Asplenium Linnaeus, 1753 is widely distributed in tropical-temperate regions and shows a high diversity. The oldest fossils assigned to it comprise fronds and spores preserved in situ from the Lower Cretaceous of northeast Asia. However, molecular dating suggests that Asplenium diverged during the Paleocene (∼57.7 Ma). Here we present some explanations for the disparity in fossil and molecular age estimates, and new insights into the origin and evolution of the genus. We suggest that Cretaceous Asplenium most likely represents a stem-group member, and that lineage extinction during the early evolutionary stages of the genus resulted in missing nodes.     

Arsenic accumulation in the roots of Helianthus annuus and Zea mays by irrigation with arsenic-rich groundwater: Insights from synchrotron X-ray fluorescence imaging

The aim of this study was to investigate the accumulation of arsenic (As) in and on roots of Zea mays (maize) and Helianthus annuus (sunflower) by means of synchrotron-based micro-focused X-ray fluorescence imaging (μ-XRF). Plant and soil samples were collected from two field sites in the Hetao Plain (Inner Mongolia, China) which have been regularly irrigated with As-rich groundwater. Detailed μ-XRF element distribution maps were generated at the Fluo-beamline of the Anka synchrotron facility (Karlsruhe Institute of Technology) to assess the spatial distribution of As in thin sections of plant roots and soil particles. The results showed that average As concentrations in the roots (14.5–27.4 mg kg⁻¹) covered a similar range as in the surrounding soil, but local maximum root As concentrations reached up to 424 mg kg⁻¹ (H. annuus) and 1280 mg kg⁻¹ (Z. mays), respectively. Importantly, the results revealed that As had mainly accumulated at the outer rhizodermis along with iron (Fe). We therefore conclude that thin crusts of Fe-(hydr)oxides cover the roots and act as an effective barrier to As, similar to the formation of Fe plaque in rice roots. In contrast to permanently flooded rice paddy fields, regular flood irrigation results in variable redox conditions within the silty and loamy soils at our study site and fosters the formation of Fe-(hydr)oxide plaque on the root surfaces.     

Holocene climatic change and the nomadic Anthropocene in Eastern Tibet: palynological and geomorphological results from the Nianbaoyeze Mountains

Our study provides detailed information on the Lateglacial landscape and vegetation development of Tibet. Based on a suite of geomorphological and palynological proxy data from the Nianbaoyeze Shan on the eastern margin of the Tibetan Plateau (33°N/101°E, 3300–4500 m asl.), we reconstruct the current state as a function of climate history and the longevity of human influence. Study results constrain several major phases of aeolian sedimentation between 50–15 ka and various glacier advances during the Late Pleistocene, the Holocene and the Little Ice Age. Increased aeolian deposition was primarily associated with periods of more extensive glacial ice extent. Fluvial and alluvial sediment pulses document an increase of erosion starting at 3926 ± 79 cal yr B.P., coinciding with cooling (Neoglacial) and a growing anthropo-zoogenic influence. Evidence for periglacial mass movements indicate that the late Holocene cooling started at around 2000 cal yr B.P., demonstrating increased surface activity under the combined effects of human influence and climate deterioration. The onset of peat growth generally depended on local conditions that include relief, meso-climate and in more recent times also on soil compaction due to animal trampling. We distinguish three initiation periods of peat growth: 12,700–10,400 cal yr B.P. for flat basins inside last glacial terminal moraines; 7000–5000 cal yr B.P. for the main valley floors; and 3000–1000 cal yr B.P. for the higher terrace surfaces.The Holocene vegetation history started with an open landscape dominated by pioneer shrubs along braided rivers (<10,600–9800 cal yr B.P.), followed by the spreading of conifers (Picea, Juniperus, Abies) and Betula-trees accompanied by a successive closing of the vegetation cover by Poaceae, Cyperaceae and herbs (9800–8300 cal yr B.P.). First signs of nomadic presence appear as early as 7200 cal yr B.P., when temperatures were up to 2 °C warmer than today. Forest remained very patchy with strong local contrasts. During the following cooling phase (5900–2750 cal yr B.P.) the natural vegetation was transformed by nomadic grazing to Bistorta-rich Kobresia pygmaea-pastures. Modern nomadic migration routes were established at least 2200 years ago. Overgrazing and trampling led to the shrinking of Bistorta and the spreading of annual weeds. Short-lived cold events (8000, 6200, 3500 cal yr B.P.) impacted on the vegetation only temporarily.As the transformation of the natural Poaceae-rich vegetation into Kobresia-pastures modified the influence of the Tibetan Plateau (“hot plate”) on the monsoon system, our data even point to an early start of a nomadic (!) Anthropocene nearly 6000 years ago. Against the background of a very long grazing history, modern Tibet must be seen as a cultural landscape.