西太平洋狗母鱼科鱼类种类多样性的研究

整理记述了西太平洋狗母鱼科Saurida、Synodus、Trachinocephalus 三属鱼类,给出了属以及主要种类的检索表,并报告了各个种的地理分布区。在此基础上,讨论了西太平洋狗母鱼科鱼类生物多样性特征、动物地理学特点及部分名称订正和资源与渔业上的意义。     

基于B-H模型的多鱼种资源评估

本文运用B—H模型对南海北部底拖网的主要渔获对象：深水金线鱼、金线鱼、马六甲绯鲤、条尾绯鲤、多齿蛇鲻、蓝圆鲹、长尾大眼鲷、短尾大眼鲷、红鳍笛鲷、二长棘鲷、黄鲷的单位补充量渔获量进行评估，分析了拖网作业对不同鱼种产生的影响，并探讨了多鱼种渔业资源管理存在的问题和对策。     

南澎列岛近海海域底拖网鱼类组成的季节性变化

1991年逐月对南澎列岛近海海域作底拖网调查,共捕获鱼类132种,其中软骨鱼类7种（4目6科6属）,硬骨鱼类125种（10目58科95属）,后 者以鲈形目居多（69种）。全年渔获优质种类为条尾鲱鲤、黑天竺鱼、二长棘鲷、大头狗母鱼、多齿蛇鲻、三线矶鲈、绿布氏筋鱼、多鳞Xi和乔氏台雅鱼等9种,其渔获尾数之和是渔获总数的71．1％。季节性优势种类除全年优势种类外,尚有粗纹Fu、半线天竺鲷、印白姑鱼、静Fu和翼红娘鱼等5种;各种类全的渔获尾数及出现月份数相关,各月份的渔获尾数和渔获种数不相关;渔获物中仅有少量的中上层鱼类（13种）。就适温性而言,该海区鱼类以亚热带性为主并兼有热带性特征,以Shannon、Brillouin、Simpson和McIn-tosh等多样性指标及相应的Hurlbert均匀度指标对逐月样本做估计,这8种指标之间高度显著相关;该海域鱼类的Shannon多样性指标及相应的均匀度指标皆以秋冬季为高,春夏季较低。     

生物和非生物因子对秋季海州湾长蛇鲻栖息地适宜性的影响

根据2011年和2013?2018年秋季在海州湾及邻近海域进行的底拖网调查数据,结合同步采集的底层水温、底层盐度、水深、资源密度、饵料生物等生物和非生物因子数据,开展长蛇鲻(Saurida elongata)栖息地适宜性的相关研究。利用提升回归树(Boosted Regression Tree, BRT)模型确定各环境因子的权重,分别采用算术平均法和几何平均法建立栖息地适宜性指数(Habitat Suitability Index, HSI)模型,并通过交叉验证确定最优模型。结果表明:海州湾长蛇鲻在秋季最适宜栖息的底层水温范围为17.5～18℃,最适底层盐度范围为31.3～32.0,最适水深范围为24～37 m;选择其3种主要饵料生物作为生物因子,即枪乌贼(Loligo spp.)、戴氏赤虾(Metapenaeopsis dalei)和六丝钝尾鰕虎鱼(Amblychaeturichthys hexanema),与底层水温、底层盐度和水深共同作为影响因子建立HSI模型。结果显示,对长蛇鲻空间分布总偏差贡献率最高的是饵料因子,其次是水深和底层水温。通过交叉验证发现,运用算术平均算法,且赋予权重的HSI模型具有较低的赤池信息准则值(Akaike Information Criterion, AIC)。研究发现,海州湾秋季长蛇鲻的最适栖息地(HSI≥0.7)主要分布在34.5°～36°N,119°～121°E之间,其中35°～36°N海域的最适栖息地分布范围大,而且从近岸至远海,HSI指数有增加的趋势。     

三峡工程蓄水前后秋季长江口鱼类浮游生物群落结构的动态变化初探

对长江口及其邻近海域2002年秋季与2003年秋季鱼类浮游生物的调查资料进行比较,分析三峡工程蓄水前后秋季长江口鱼类浮游生物群落结构特征变化.结果发现,蓄水后鱼类浮游生物种类和数量比蓄水前有所增加,群落结构和时空分布特征发生了明显的改变,一些分布在黄渤海的种类如方氏云ND022等开始在东海出现,并且部分鱼类的生殖时间上也发生了改变.利用相对重要性指数（IRI）对长江口鱼类浮游生物进行优势度检验,其优势种成分也发生了很大的变化,虽仍是以小型种类为主,但原占绝对优势的日本ND023优势度开始逐渐下降,成为常见种,而康氏小公鱼的数量急剧上升.阐明了长江口鱼类资源的补充及群落结构和多样性变化规律及其与环境的关系,为动态监测三峡工程对河口生态环境的影响提供科学依据.     

鱼油脂肪酸组成及其含量分析

分析测定了鱼是鱼鱼油的脂肪酸组成、含量及分布范围。脂肪酸组成从１４碳到２２碳。其中具有生理功能活性物质的二十碳五烯酸（ＥＰＡ）、二十二碳六烯酸（ＤＨＡ）占总量的２１％左右。比较了东海１４种鱼的鱼油中ＥＰＡ、ＤＨＡ含量的高低,得出鱼是鱼鱼油中的ＥＰＡ、ＤＨＡ含量除低于鲣鱼外,均高于乌贼、小黄鱼、白姑鱼、银鲳鱼、鳓鱼和黄姑鱼等名贵海鱼。     

Two new species of Ectopleura from the Taiwan Strait, China (Cnidaria, Hydroidomedusae)

Two new species of genus Ectopleura, i.e., Ectopleura elongata sp. nov. and E. triangularis sp. nov. are described in the present paper based on type specimens from the Taiwan Strait. The types are deposited in the Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.     

应用CEDA和ASPIC软件包对巴基斯坦国的多齿蛇鲻(Saurida tumbil)渔业的最大持续产量的初步研究

The catch and effort data analysis(CEDA) and ASPIC(a stock assessment production model incorporating covariates) computer software packages were used to estimate the maximum sustainable yield(MSY) from the catch and effort data of Greater lizardfish Saurida tumbil fishery of Pakistan from 1986 to 2009. In CEDA three surplus production models of Fox, Schaefer and Pella-Tomlinson were used. Here initial proportion(IP) of 0.5 was used because the starting catch was roughly 50% of the maximum catch. With IP = 0.5, the estimated MSY from Fox model were 20.59 mt and 38.16 mt for normal and log-normal error assumptions, while the MSY from Schaefer and Pella-Tomlinson were 60.40, 60.40 and 60.40 mt, for normal, log-normal and gamma error assumptions respectively. The MSY values from Schaefer and Pella-Tomlinson models of three error assumptions were the same. The R2 values from those three models were above 0.6. When IP = 0.5, the MSY values estimated from ASPIC from Fox were 132 mt, and from logistic model were 69.4 mt, with R2 value above 0.8. Therefore we suggest the MSY of S. tumbil fishery from Pakistan to be 60–70 mt, which is higher than the latest catch, thus we would recommend that the fishing efforts for this fishery may be kept at the current level.     

高体(鱼狮)(Seriola dumerili)线粒体全基因组测定及结构特征分析

为快速有效鉴别(鱼师)属鱼类物种、加强(鱼师)鱼遗传多样性管理与种质资源保护,通过Illumina测序技术,获得了东海海域养殖高体(鱼师)(Seriola dumerili)线粒体基因组全序列(16 530bp),碱基组成为A (26.83%)、G (17.6%)、C (30.04%)和T (25.53%), A+T含量为52.36%,且非编码控制区(D-loop)A+T富含61.64%,表现明显的AT偏好性。与其他硬骨鱼一样,高体(鱼师)线粒体基因组包含13条蛋白编码基因, 22个tRNA基因, 2个rRNA基因,除ND6、tRNA^Gln、tRNA^Ala、tRNA^Asn、tRNA^Cys、tRNA^Tyr、tRNA^Ser、tRNA^Glu、tRNA^Pro基因外,其余均位于H链编码;蛋白编码基因中,除COⅠ、COⅡ和ND5的起始密码子分别为ATC、ATA和ATA外,其余10个蛋白编码基因的起始密码子均为ATG,以典型的TAA和TAG为终止密码子,在ND4和Cytb中存在不完全密码子T;除tRNA^Ser-GCT外,其余21个tRNA均为典型三叶草二级结构。比较中国和日本海域高体(鱼师)线粒体基因组发现, CO Ⅰ、CO Ⅱ和ND5蛋白编码基因在起止位置、片段长度及起止密码子上存在显著差异。此外,与同属的黄条(Seriola aureovittata)和五条(鱼师)(Seriola quinqueradiata)的线粒体基因组13个蛋白编码基因进行两两对比分析,结果表明3种(鱼师)属鱼类的蛋白编码基因的相似性在85%~100%之间。基于线粒体基因组全序列构建的系统发育树,成功将高体(鱼师)与其他(鱼师)属鱼类有效区分,高体(鱼师)与长鳍(鱼师)同属一支,亲缘关系最近;黄条(鱼师)和五条(鱼师)聚为一支,亲缘关系最近。     

中国沿海戈芬星虫属(星虫动物门)的研究

戈芬星虫属隶属于方格星虫纲、戈芬星虫目、戈芬星虫科,世界共报道10种,其中2个种各有2个亚种。本文报道我国沿海戈芬星虫属共计3种,即:珠光戈芬星虫、长戈芬星虫和普通戈芬星虫。     

厦门沿岸海域渔业资源变化和最适捕捞力量的估算

本文以厦门沿岸海域历次渔业资源和生物调查及１９８４－１９９５年渔业统计资料为背景,分析了该海域渔业资源的变动,重点对文昌鱼、真鲷、鳓鱼、大黄鱼、蓝点斑马鲛、长毛对、中国鲎等重要经济种类资源变动的原因进行探讨,针对目前资源的变动趋向,指出了近期重点的保护对象和有开发潜力的种类,并以三种标准捕捞力量,应用了Ｓｃｈａｅｆｅｒ和Ｆｏｘ两种剩余产量模式估算最大持续产量（ＭＳＹ）和最知捕捞力量。估算结果ＭＳＹ     

盐度渐变对黄条鰤消化酶和超氧化物歧化酶活力及甲状腺激素的影响

为探讨黄条鰤(Seriola aureovittata)对盐度渐变的应激响应机制,设置盐度5、10、15、20、29和35共6个盐度渐变点,对幼鱼的消化酶活力、超氧化物歧化酶(SOD)和甲状腺激素(T4)等生理指标进行了检测和分析。研究显示:胃、肠、肝脏和幽门盲囊的脂肪酶的活性均在盐度29渐变点最高,在低盐度渐变点这4个组织的脂肪酶活性均随盐度降低而降低。肠和肝脏的蛋白酶活性在盐度29渐变点达到峰值,幽门盲囊的蛋白酶活性则在盐度35渐变点最高,且与其他盐度渐变点间有显著性差异(P<0.05)。胃、肠、肝脏的淀粉酶活性在盐度29渐变点最高,而幽门盲囊的淀粉酶活性在盐度35渐变点最高,但与盐度29渐变点无显著性差异(P>0.05)。血清SOD活性在盐度5渐变点显著低于其他盐度渐变点(P<0.05),SOD活性在盐度20和35渐变点与29渐变点无显著性差异(P>0.05)。血清T4浓度随着盐度的升高或降低均升高,盐度20渐变点与29渐变点的T4浓度无显著性差异(P>0.05)。研究显示,自然海水盐度29是黄条鰤幼鱼存活的适宜盐度,且黄条鰤幼鱼能较快适应略低盐度(20~29),但较低或较高盐度渐变点的消化酶活力和抗应激指标则显著异常。盐度渐变可引起黄条鰤幼鱼的消化生理、抗氧化水平和T4浓度的变化,黄条鰤幼鱼对外界盐度变化表现出较强的适应性。     

北部湾主要经济鱼类资源变动分析及保护对策探讨

运用北部湾历史上几次底拖网渔业资源调查资料．对14种主要经济鱼类的资源变动情况进行综合分析。结果表明．曾是20世纪60年代底拖网渔获优势种的红笛鲷(Lutjanus sanguineus))、鯻(Therapon theraps)、长棘银鲈(Gerres filamentosus)、黑印真鲨(Carcharhinus menisorrah)和灰裸顶鲷(Gymnocranius griseus)等资源已严重衰竭；二长棘鲷(Parargyrops edita)、长尾大眼鲷(Priacanthus tayenus)、短尾大眼鲷(Priacanthus macracanthus)、白姑鱼(Argyrosomus argentatus)、蓝圆鲹(Decapterus maruadsi)、竹荚鱼(Trachurus japonicus)等种类的资源密度的波动较大：带鱼(Trichiurus haumela)、花斑蛇鲻(Saurida undosquamis)、刺鲳(Psenopsis anomala)等资源虽呈衰退趋势．但资源密度相对较为稳定。并探讨了过度捕捞对不同种类资源变动的影响及渔业资源的保护对策。     

南海Argo浮标观测结果初步分析

对投放在南海内部的4个Argo浮标轨迹特征,温、盐结构及漂移速度进行诊断分析发现,Argo浮标剖面温、盐观测结果和气候态资料一致,同时上层海洋温、盐剖面资料表现出周期约2月的扰动信号,并且温跃层起伏与同期海表高度异常相关显著。和高度计资料计算而得的海表地转流相比,由Argo浮标漂移轨迹计算的表观流量值较小。在季节时间尺度上,Argo浮标表观流和Argo浮标所停留的中层参考面季节性海流相一致。     

The intermediate Mediterranean water inflow along the northern slope of the Iberian Peninsula

During the SEFOS 95 cruise (25 March to 15 April 1995) (figure 1) a number of CTD casts were made in order to study the intermediate Mediterranean water (IMW) off the west and north coasts of the Iberian Peninsula. One long-term mooring, for current measurement at four levels, was deployed at 43° 57′ N - 5° 51′ W from February to May. We establish a picture of the distribution and transformation of the IMW along the coast of the Iberian Peninsula based on the thermohaline characteristics of the water masses and the determination of the percentage content of the IMW by the use of the triangle of vertical mixing of the North Atlantic central water (NACW), IMW and Labrador water (LW) [3]. A generalised temperature/salinity (T/S) curve is plotted (figure 6) on the basis of the T/S curves of the cruise. The results of 3 months' current measurements are shown.     

Identification of Provocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified,cloned and sequenced,and these sequence data were deposited in the GenBank.Eight oligonucleotide probes(DNA probes)were designed based on the sequence analysis.The probes were employed to detect and identify P.minimum and T. pulchella in unialgal and mixed algal samples with a fuorescence in situ hybridization method using flow cytometry.Epifluorescence micrographs showed that these specific probes labeled with fluorescein isothiocyanate entered the algal cells and bound to target sequences,and the fluorescence signal resulting from whole-cell hybridization varied from probe to probe.These DNA probes and the hybridization protocol we developed were specific and effective for P.minimum and T. pulchella,without any specific binding to other algal species.The hyrbridization efficiency of difierent probes specific to P.minimum was in the order:PMl8S02＞PM28S02＞PM28S01＞PM18S01,and that of the probes specific to T. pulchella was TP18S02＞TP28S01＞TP28S02＞TP18S01.The djfferent hybridization efficiency of the DNA probes could also be shown in the fuorescent signals between the labeled and unlabeled cells demonstrated using flow cytometry.The DNA probes PM18S02,PM28S02,TPl8S02 and TP28S01,and the protocol,were also useful for the detection of algae in natural samples.     

全球大洋潮汐模式在北印度洋潮汐预报准确性的评估

为评估DTU10、TPXO8、GOT00.2和NAO.99b 4个全球大洋潮汐模式对北印度洋潮汐的预报能力,采用英国海洋资料中心提供的海区中部和沿岸站潮汐调和常数资料,检验了这些模式4个主要分潮(M_2、S_2、K_1、O_1)的准确度。它们的各分潮调和常数资料准确度都比较高,振幅绝均差的最大值仅5.61 cm,迟角绝均差的最大值仅9.13°。这些模式的调和常数给出潮波传播特征差别不大。基于这些模式提供的调和常数,分别建立了北印度洋4、8和16分潮潮汐预报模型,将预报结果与中国海事服务网提供的沿岸24个站潮汐表资料进行对比。各模式的8分潮(M_2、S_2、N_2、K_2、K_1、O_1、P_1、Q_1)潮汐预报模型均优于4分潮(M_2、S_2、K_1、O_1)潮汐预报模型,NAO.99b模式可以提供16分潮(M_2、S_2、N_2、K_2、K_1、O_1、P_1、Q_1、MU_2、NU_2、T_2、L_2、2N_2、J_1、M1、OO_1)潮汐预报模型,但是对预报结果改善不明显;在各模式中,GOT00.2模式的8分潮潮汐预报模型对北印度洋沿岸的预报效果最好,平均绝均差为14.97 cm。     

Effect of Salinity on Estimating Geostrophic Transport of the Indonesian Throughflow along the IX1 XBT Section

Geostrophic transport of the Indonesian Throughflow (ITF) is estimated from optimally-interpolated temperature data along a frequently repeated expendable bathythermograph (XBT) section between Fremantle, Australia and Sunda Strait, Indonesia and from two historical temperature-salinity (T/S) relationship products, CSIRO Atlas for Regional Seas (CARS) and Levitus (1982). The annual mean ITF geostrophic transport relative to 400 m during 1984–2001 is estimated to be 4.6 Sv using the CARS T/S relationship, which is about 20% higher than that found using the Levitus T/S relationship. This transport increment is due to the fact that the CARS T/S relationship, which incorporates more recent hydrographic data, better resolves the low-salinity signature of the ITF water. Isothermal averaging in the CARS T/S relationship may also improve representations of the water mass signatures in deep layers.     

Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified,cloned and sequenced,and these sequence data were deposited in the GenBank.Eight oligonucleotide probes(DNA probes)were designed based on the sequence analysis.The probes were employed to detect and identify P.minimum and T. pulchella in unialgal and mixed algal samples with a fuorescence in situ hybridization method using flow cytometry.Epifluorescence micrographs showed that these specific probes labeled with fluorescein isothiocyanate entered the algal cells and bound to target sequences,and the fluorescence signal resulting from whole-cell hybridization varied from probe to probe.These DNA probes and the hybridization protocol we developed were specific and effective for P.minimum and T. pulchella,without any specific binding to other algal species.The hyrbridization efficiency of difierent probes specific to P.minimum was in the order:PMl8S02＞PM28S02＞PM28S01＞PM18S01,and that of the probes specific to T. pulchella was TP18S02＞TP28S01＞TP28S02＞TP18S01.The djfferent hybridization efficiency of the DNA probes could also be shown in the fuorescent signals between the labeled and unlabeled cells demonstrated using flow cytometry.The DNA probes PM18S02,PM28S02,TPl8S02 and TP28S01,and the protocol,were also useful for the detection of algae in natural samples.     

闽江口硅藻-盐度转换函数建立

目前国内利用定量分析的方法研究河口区古环境屈指可数,闽江口尚缺乏相关研究,本研究旨在闽江口建立硅藻-盐度转换函数,为今后研究闽江口古环境提供科学依据。在对闽江口表层沉积硅藻进行CCA分析后,结果表明盐度对硅藻属种变化具有最大解释量,并在研究区划分出4个区,Ⅰ区硅藻分布受河口外沿岸水体影响;Ⅱ区硅藻分布受潮汐上溯海水入侵影响;Ⅲ区硅藻分布受到潮汐与径流共同影响;Ⅳ区硅藻分布主要受径流影响。通过剔除异常站位及模型比选等手段确定最优硅藻-盐度转换函数为WA-PLS模型下Component 5模型,转换函数关系式为S拟=0. 25+1. 007S实,并以2. 76的平均误差作为补偿提升转换函数精度。