Hydrogen isotopes in dinosterol from the Chesapeake Bay estuary

The hydrogen isotope ratio of the dinoflagellate sterol dinosterol (4α,23,24-trimethyl-5α-cholest-22E-en-3β-ol) was measured in suspended particles and surface sediments from the Chesapeake Bay estuary in order to evaluate the influence of salinity on hydrogen isotope fractionation. D/H fractionation was found to decrease by 0.99 ± 0.23‰ per unit increase in salinity over the salinity range 10-29 PSU, a similar decrease to that observed in a variety of lipids from hypersaline ponds on Christmas Island (Kiribati). We hypothesize that the hydrogen isotopic response to salinity may result from diminished exchange of water between algal cells and their environment, lower growth rates and/or increased production of osmolytes at high salinities. Regardless of the mechanism, the consistent sign and magnitude of dinosterol δD response to changing salinity should permit qualitative to semi-quantitative reconstructions of past salinities from sedimentary dinosterol δD values.     

The sedimentary record of climatic and anthropogenic influence on the Patuxent estuary and Chesapeake Bay ecosystems

Ecological and paleoecological studies from the Patuxent River mouth reveal dynamic variations in benthic ostracode assemblages over the past 600 years due to climatic and anthropogenic factors. Prior to the late 20th century, centennial-scale changes in species dominance were influenced by climatic and hydrological factors that primarily affected salinity and at times led to oxygen depletion. Decadal-scale droughts also occurred resulting in higher salinities and migration of ostracode species from the deep chanel (Loxoconcha sp.,Cytheromorpha newportensis) into shallower water along the flanks of the bay. During the 19th century the abundance ofLeptocythere nikraveshae andPerissocytheridea brachyforma suggest increased turbidity and decreased salinity. Unprecedented changes in benthic ostracodes at the Patuxent mouth and in the deep channel of the bay occurred after the 1960s whenCythermorpha curta became the dominant species, reflecting seasonal anoxia. The change in benthic assemblages coicided with the appearance of deformities in foraminifers. A combination of increased nitrate loading due to greater fertilize use and increased fresh-water flow explains this shift. A review of the geochemical and paleoecological evidence for dissolved oxygen indicates that seasonal oxygen depletion in the main channel of Chesapeake Bay varies over centennial and decadal timescales. Prior to 1700 AD, a relatively wet climate and high freshwater runoff led to oxygen depletion but rarely anoxia. Between 1700 and 1700, progressive eutrophication occurred related to land clearance and increased sedimentation, but this was superimposed on the oscillatory pattern of oxygen depleton most likely driven by climatological and hydrological factors. It also seems probable that the four-to five-fold increase in sedimentation due to agricultural and timber activity could have contributed to an increased natural nutrient load, likely fueling the early periods (1700–1900) of hypoxia prior to widespread fertilizer use. Twentieth-century anoxia worsened in the late 1930s–1940s and again around 1970, reaching unprecedented levels in the past few decades. Decadal and interannual variability in oxygen depletion even in the 20th century is still strongly influenced by climatic processes influencing precipitation and freshwater runoff.     

Seasonal oxygen depletion in Chesapeake Bay

The spring freshet increases density stratification in Chesapeake Bay and minimizes oxygen transfer from the surface to the deep layer so that waters below 10 m depth experiece oxygen depletion which may lead to anoxia during June to September. Respiration in the water of the deep layer is the major factor contributing to oxygen depletion. Benthic respiration seems secondary. Organic matter from the previous year which has settled into the deep layer during winter provides most of the oxygen demand but some new production in the surface layer may sink and thus supplement the organic matter accumulated in the deep layer.     

Fish biomass size spectra in Chesapeake Bay

Biomass size spectra of pelagic fish were modeled to describe community structure, estimate potential fish production, and delineate trophic relationships in Chesapeake Bay. Spectra were constructed from midwater trawl collections each year in April, June–August, and October 1995–2000. The size spectra were bimodal: the first spectral dome corresponded to small zooplanktivorous fish, primarily bay anchovyAnchoa mitchilli; the second dome consisted of larger fish from several feeding guilds that are supported by multiple prey-predator linkages. Annual production estimates of pelagic fish, derived from a mean production to biomass ratio, varied nearly three-fold, ranging from 162 × 109 kcal (125 × 103 tons) in 1996 to 457 × 109 kcal (352 × 103 tons) in 2000. Seasonally, the biomass level and mean individual sizes of fish in the first dome increased from April to October, while the biomass level of the second dome was relatively stable. Regionally, biomass levels in the second dome were higher than biomasses in the first dome for the upper and lower Bay, but were minimal in the middle Bay where seasonal and episodic hypoxia occurs. To test a benthic-pelagic coupling hypothesis that could explain the higher biomass in the second domes for the lower and upper Bay, a cyclic size-spectrum model was fit that included only species in the zooplanktivorous-piscivorous fish guilds. The mean, normalized slope equaled ?1, indicating that zooplanktivorous fish may support piscivore production, but that a benthic-pelagic linkage is required to fully support fish production in the second dome. Interannual variability in slopes and intercepts of modeled size spectra was related to salinity, recruitment level of bay anchovy, and the primary axis of a correspondence analysis (salinity effect) on fish community structure. The spectral slope and intercept of normalized spectra were lowest in 1996, a near-record wet year. Results suggest that fish size spectra can be developed as useful indicators of ecosystem state and response to perturbations, especially if prey-predator relationships are explicitly represented.     

Modeling of wind-induced destratification in Chesapeake Bay

It has been observed that storms in early fall can result in top-to-bottom mixing of Chesapeake Bay. A three-dimensional, time-dependent circulation model is used to examine this destratification process for September 1983, when extensive current and hydrographic data were available. The model bay is forced at the surface by observed hourly winds, at the ocean boundary by observed hourly surface and bottom salinities and sea level fluctuations, and at the head by observed daily discharges for a 28-d period. A second-moment, turbulence-closure submodel, with no adjustments from previous applications to its requisite coefficients, is used to calculate the vertical turbulence mixing coefficients. Comparisons with data inside the model domain indicate relative errors of 7% to 14% for sea level, 7% to 35% for current, and 11% to 21% for salinity. The tidal portion of the spectrum is modeled better than the subtidal portion. The model is used to examine both the mechanisms of wind mixing and the temporal and spatial distribution of vertical mixing within the estuary. Wind-driven internal shear is shown to be a more effective mechanism of inducing destratification than turbulence generated at the surface. The model is also used to show that the vertical temperature inversion which occurs in the fall does not affect the timing of the destratification as much as its completeness. The distribution of mid-depth vertical mixing shows highly variable values in the mid-bay region, where wind-induced mixing is dominant. This suggests that the source of oxygen to mid-bay bottom waters is similarly variable. Vertical turbulence mixing coefficients of 10^?2 cm² s^?1 (background) to 10³ cm² s^?1 were needed to simulate the September period, indicating the need for time-variable mixing in models of dissolved and suspended estuarine constituents.     

Turbidity Maximum Entrapment of Phytoplankton in the Chesapeake Bay

Estuarine turbidity maxima (ETM) play an important role in zooplankton and larval fish productivity in many estuaries. Yet in many of these systems, little is known about the food web that supports this secondary production. To see if phytoplankton have the potential to be a component of the ETM food web in the Chesapeake Bay estuary a series of cruises were carried out to determine the biomass distribution and floral composition of phytoplankton in and around the ETM during the winter and spring using fluorometry, high-performance liquid chromatography (HPLC), and microscopy. Two distinct phytoplankton communities were observed along the salinity gradient. In lower salinity waters, biomass was low and the community was composed mostly of diatoms, while in more saline waters biomass was high and the community was composed mostly of mixotrophic dinoflagellates, which were often concentrated in a thin layer below the pycnocline. Phytoplankton biomass was always low in the ETM, but high concentrations of phytoplankton pigment degradation products and cellular remains were often observed suggesting that this was an area of high phytoplankton mortality and/or an area where phytoplankton derived particulate organic matter was being trapped. These results, along with a box model analysis, suggest that under certain hydrodynamic conditions phytoplankton derived organic matter can be trapped in ETM and potentially play a role in fueling secondary production.     

The occurrence of fungi on some rooted aquatics from the Chesapeake Bay

During 1975 and 1976, 433 isolates, representing 37 genera of fungi, were collected fromPotamogeton perfoliatus andRuppia maritima in the Chesapeake Bay. Seasonal distribution patterns of selected species are presented and their possible relationships to the declining host plant populations are discussed.     

Identification of important primary producers in a Chesapeake Bay tidal creek system using stable isotopes of carbon and sulfur

The use of multiple stable isotopes in the study of trophic relationships in temperate estuaries has usually been limited to euhaline systems, in which phytoplankton, benthic microalgae, andSpartina alterniflora are major sources of organic matter for consumers. Within large estuaries such as Chesapeake Bay, however, many species of consumers are found in the upper mesohaline to oligohaline portions. These lower salinity wetlands have a greater abundance of macrophytes that use C3 photosynthesis to fix carbon, in addition toS. alterniflora, which fixes carbon via the C4 photosynthetic pathway. In a broad survey of the biota and sediments of a brackish tidal creek tributary to Chesapeake Bay, combined δ¹³C and δ³⁴S measurements disclosed a balanced contribution to secondary production from phytoplankton, C3 macrophytes,Spartina sp., and benthic microalgae. Surface sediment δ¹³C suggested that the organic matter from C3 plants was derived both from allochthonous sources (terrestrial runoff) and from autochthonous production (marsh macrophytes). Unlike most estuarine systems studied to date, which are dominated by algae (phytoplankton and benthic microalgae) and C4 macrophytes, C3 plants are of greater importance in the diets of consumers in this low-salinity creek system.     

Speciation of aluminum in the stream waters from the Susquehanna River watershed, Chesapeake Bay

 The aim of this paper was to analyze the processing and transport of aluminum speciation through the terrestrial and aquatic ecosystems of the Chesapeake Bay landscape. Stream waters were sampled in 14 different streams from the Susquehanna River watershed to the upper reach of Chesapeake Bay. The basic water quality data were documented and Al speciation was evaluated by modified MINEQL computer model. It was found that the increase in acidity in some of the stream waters near New York were closely linked with a marked decline in basic cation concentrations. In these areas, the sum of the toxic forms of Al³⁺ plus AlOH²⁺ and Al(OH)₂ ⁺ concentrations exceeds the critical level of 5 μmol l^–1. A potential threat from aluminum may occur. Received: 3 January 2000 · Accepted: 21 March 2000     

Wind Modulation of Dissolved Oxygen in Chesapeake Bay

A numerical circulation model with a simplified dissolved oxygen module is used to examine the importance of wind-driven ventilation of hypoxic waters in Chesapeake Bay. The model demonstrates that the interaction between wind-driven lateral circulation and enhanced vertical mixing over shoal regions is the dominant mechanism for providing oxygen to hypoxic sub-pycnocline waters. The effectiveness of this mechanism is strongly influenced by the direction of the wind forcing. Winds from the south are most effective at supplying oxygen to hypoxic regions, and winds from the west are shown to be least effective. Simple numerical simulations demonstrate that the volume of hypoxia in the bay is nearly 2.5 times bigger when the mean wind is from the southwest as compared to the southeast. These results provide support for a recent analysis that suggests much of the long-term variability of hypoxia in Chesapeake Bay can be explained by variations in the summertime wind direction.     

Dissolved and particulate organic carbon in Chesapeake Bay

We measured dissolved and particulate organic carbon (DOC and POC) in samples collected along 13 transects of the salinity gradient of Chesapeake Bay. Riverine DOC and POC end-members averaged 232±19 μM and 151±53 μM, respectively, and coastal DOC and POC end-members averaged 172±19 μM and 43±6 μM, respectively. Within the chlorophyll maximum, POC accumulated to concentrations 50–150 μM above those expected from conservative mixing and it was significantly correlated with chlorophylla, indicating phytoplankton origin. POC accumulated primarily in bottom waters in spring, and primarily in surface waters in summer. Net DOC accumulation (60–120 μM) was observed within and downstream of the chlorophyll maximum, primarily during spring and summer in both surface and bottom waters, and it also appeared to be derived from phytoplankton. In the turbidity maximum, there were also net decreases in chlorophylla (?3 μg l^?1 to ?22 μg l^?1) and POC concentrations (?2 μM to ?89 μM) and transient DOC increases (9–88 μM), primarily in summer. These occurred as freshwater plankton blooms mixed with turbid, low salinity seawater, and we attribute the observed POC and DOC changes to lysis and sedimentation of freshwater plankton. DOC accumulation in both regions of Chesapeake Bay was estimated to be greater than atmospheric or terrestrial organic carbon inputs and was equivalent to ≈10% of estuarine primary production.     

Scales of nutrient-limited phytoplankton productivity in Chesapeake Bay

The scales on which phytoplankton biomass vary in response to variable nutrient inputs depend on the nutrient status of the plankton community and on the capacity of consumers to respond to increases in phytoplankton productivity. Overenrichment and associated declines in water quality occur when phytoplankton growth rate becomes nutrient-saturated, the production and consumption of phytoplankton biomass become uncoupled in time and space, and phytoplankton biomass becomes high and varies on scales longer than phytoplankton generation times. In Chesapeake Bay, phytoplankton growth rates appear to be limited by dissolved inorganic phosphorus (DIP) during spring when biomass reaches its annual maximum and by dissolved inorganic nitrogen (DIN) during summer when phytoplankton growth rates are highest. However, despite high inputs of DIN and dissolved silicate (DSi) relative to DIP (molar ratios of N∶P and Si∶P>100), seasonal accumulations of phytoplankton biomass within the salt-intruded-reach of the bay appear to be limited by riverine DIN supply while the magnitude of the spring diatom bloom is governed by DSi supply. Seasonal imbalances between biomass production and consumption lead to massive accumulations of phytoplankton biomass (often>1,000 mg Chl-a m^?2) during spring, to spring-summer oxygen depletion (summer bottom water <20% saturation), and to exceptionally high levels of annual phytoplankton production (>400 g m^?2 yr^?1). Nitrogen-dependent seasonal accumulations of phytoplankton biomass and annual production occur as a consequence of differences in the rates and pathways of nitrogen and phosphorus cycling within the bay and underscore the importance of controlling nitrogen inputs to the mesohaline and lower reaches of the bay.     

Discovery of the “Phantom” dinoflagellate in Chesapeake Bay

Since its discovery in natural estuarine habitat of North Carolina in 1991, the widespread impact of the toxic dinoflagellate, Pfiesteria piscicida (gen. et sp. nov.), popularly called the “phantom” dinoflagellate, on North Carolina fish stocks has been established, yet little is known about its influence outside of North Carolina estuaries. Here, we document the presence of P. piscicida in Chesapeake Bay. A fish kill was observed after inoculating an aquarium containing mummichogs with sediment samples from Jenkins Creek, a brackish creek (salinity 11‰) of the Chesapeake Bay system. P. piscicida was the cause of the kill, as supported by morphological, physiological, and histological evidence. The appearance and behavior of the algae and symptoms associated with fish mortality were consistent with those previously observed in P. piscicida-associated aquaria fish kills in North Carolina. The discovery of P. piscicida in Chesapeake Bay supports the speculation that these toxic dinoflagellates have a dramatic and far-reaching impact on fish stocks in shallow, eutrophic estuaries along the eastern United States.     

Transient hydrodynamic and salinity simulations in the Chesapeake Bay network

A transient network model is applied to the Chesapeake Bay and its tributary estuaries. Calibration of the model is based on only three external parameters: a friction factor that is spatially described, and two global constants required to calibrate a dynamic dispersion relationship that depends on both the local salinity gradient and hydraulic conditions. The transient hydrodynamics and the transient salinity distribution of the Bay and its tributary estuaries are simulated for the period of one month and comparisons made between calculated and observed salinities.     

Distribution ofVibrio parahaemolyticus in Chesapeake Bay during the summer season

The distribution ofVibrio parahaemolyticus in Chesapeake Bay during the warmer weather of the summer months was examined. This species was found throughout the Chesapeake Bay and its tributaries, even in areas of very low salinity. Counts of this species ranged from 0.04 per 100 ml to 46 per 100 ml in the water column and 2.03 to ≥2.4×10³ per 100 cc of sediment. A variety of physical, chemical and bacteriological properties associated with the incidence and distribution ofV. parahaemolyticus were examined and salinity was found to be the major influence among the factors examined. Correlation and regression analysis showed that the population size of this species increased with increasing salinity in the estuary.     

Environmental Models and Public Stakeholders in the Chesapeake Bay Watershed

The Chesapeake Bay is not only North America's largest estuary, but it is also home to one of most comprehensive computational modeling efforts that seeks to study and integrate a very large range of the complex socio-ecological dynamics within its watershed. Known as the Chesapeake Bay Modeling System (CBMS), this suite of models are invaluable to scientists, environmental policymakers, resource managers, and local government and community leaders, all of whom are engaged in efforts to reduce the negative impacts of population growth and development in the watershed on the ecosystems and living resources of the Chesapeake Bay. Until recently, the results of the CBMS were used to guide voluntary efforts to reduce nutrient and sediment runoff into the Chesapeake Bay. However, the results are now being used to guide and evaluate the implementation of Watershed Implementation Plans at the state and county levels, which in turn are based on CBMS estimates of total maximum daily loads (TMDLs) for these sub-watershed regions. The use of the CBMS for these regulatory efforts has also increased the number of public stakeholders who are now able to directly inquire about the how the CBMS works and raise questions about its ability to accurately represent their land use decisions and practices. This "going public" of the CBMS has raised many societal and cultural issues, including the articulation of local expert and scientific knowledge, and modelers, scientists, policymakers, and resource managers are now realizing the need to understand more of the human dimensions arising from the translation and implementation of the CBMS. A multidisciplinary approach is needed to understand these rapidly emerging societal and cultural dimensions of the expanded use of the CBMS. In this paper, we draw upon our collective experience as social and natural scientists, with modeling experience, to describe a range of social, economic, political, and cultural issues that have emerged as a result of the CBMS being used to support mandatory nutrient reduction regulations (TMDL).     

Barium isotopes in individual presolar silicon carbide grains from the Murchison meteorite

Barium isotopic compositions of single 2.3-5.3 μm presolar SiC grains from the Murchison meteorite were measured by resonant ionization mass spectrometry. Mainstream SiC grains are enriched in s-process barium and show a spread in isotopic composition from solar to dominantly s-process. In the relatively coarse grain size fraction analyzed, there are large grain-to-grain variations of barium isotopic composition. Comparison of single grain data with models of nucleosynthesis in asymptotic giant branch (AGB) stars indicates that the grains most likely come from low mass carbon-rich AGB stars (1.5 to 3 solar masses) of about solar metallicity and with approximately solar initial proportions of r- and s-process isotopes. Measurements of single grains imply a wide variety of neutron-to-seed ratios, in agreement with previous measurements of strontium, zirconium and molybdenum isotopic compositions of single presolar SiC grains.     

A sediment chronology of the eutrophication of Chesapeake Bay

Chesapeake Bay sediments were examined for biogeochemical evidence of eutrophication trends using two mesohaline sediment cores. Measurements of ²¹⁰Pb geochronology and sediment profiles of organic carbon, nitrogen, organic phosphorus, inorganic phosphorus, and biogenis silica (BSi) were used used to develop temporal concentration trends. Recent sediments have 2–3 times as much organic carbon and nitrogen as sediments from 80 to 100 yr ago, but the increases result from both changes in organic matter deposition and time-dependent changes in organic matter decomposition rates. Despite increases in phosphorus loading, no major changes in phosphorus concentration were noted throughout most of the century; anthropogenic phosphorus deposition, though not evident in sulfidic mid-bay sediments, must occur in more oxidizing sediment environments in both the northern and southern bays. Temporal trends in BSi concentrations are much less evident and the lack of substantial increases in this century suggest that BSi inputs may be capped by late spring-summer Si limitation.     

Long-term pCO2 dynamics in rivers in the Chesapeake Bay watershed

Streams and rivers are major exporters of C and other dissolved materials from watersheds to coastal waters. In streams and rivers, substantial amounts of terrigenous organic C is metabolized and degassed as CO₂ to the atmosphere. A long-term evaluation of CO₂ dynamics in streams is essential for understanding factors controlling CO₂ dynamics in streams in response to changes in climate and land-use. Long-term changes in the partial pressure of CO₂ (pCO₂) were computed in the Anacostia River and the lower Potomac River in the Chesapeake Bay watershed. Long-term estimates were made using routine monitoring data of pH, total alkalinity, and dissolved nutrients from 1985 to 2006 at 14 stations. Longitudinal variability in pCO₂ dynamics was also investigated along these rivers downstream of the urban Washington D.C. metropolitan area. Both rivers were supersaturated with CO₂ with respect to atmospheric CO₂ levels (392 μatm) and the highly urbanized Anacostia waters (202–9694 μatm) were more supersaturated than the Potomac waters (557–3800 μatm). Long-term variability in pCO₂ values may be due to changes in river metabolism and organic matter and nutrient loadings. Both rivers exchange significant amounts of CO₂ with the atmosphere (i.e., Anacostia at 0.2–72 mmol m⁻² d⁻¹ and Potomac at 0.12–24 mmol m⁻² d⁻¹), implying that waterways receiving organic matter and nutrient subsidies from urbanized landscapes have the potential to increase river metabolism and atmospheric CO₂ fluxes along the freshwater–estuarine continuum.     

Reconsidering the physics of the Chesapeake Bay estuarine turbidity maximum

A series of cruises was carried out in the estuarine turbidity maximum (ETM) region of Chesapeake Bay in 1996 to examine physical and biological variability and dynamics. A large flood event in late January shifted the salinity structure of the upper Bay towards that of a salt wedge, but most of the massive sediment load delivered by the Susquehanna River appeared to bypass the ETM zone. In contrast, suspended sediments delivered during a flood event in late October were trapped very efficiently in the ETM. The difference in sediment trapping appeared to be due to increases in particle settling speed from January to October, suggesting that the fate of sediments delivered during large events may depend on the season in which they occur. The ETM roughly tracked the limit of salt (defined as the intersection of the 1 psu isohaline with the bottom) throughout the year, but it was often separated significantly from the limit of salt with the direction of separation unrelated to the phase of the tide. This was due to a lag of ETM sediment resuspension and transport behind rapid meteorologically induced or river flow induced motion of the salt limit. Examination of detailed time series of salt, suspended sediment, and velocity collected near the limit of salt, combined with other indications, led to the conclusion that the convergence of the estuarine circulation at the limit of salt is not the primary mechanism of particle trapping in the Chesapeake Bay ETM. This convergence and its associated salinity structure contribute to strong tidal asymmetries in sediment resuspension and transport that collect and maintain a resuspendable pool of rapidly settling particles near the salt limit. Without tidal resuspension and transport, the ETM would either not exist or be greatly weakened. In spite of this repeated resuspension, sedimentation is the ultimate fate of most terrigenous material delivered to the Chesapeake Bay ETM. Sedimentation rates in the ETM channel are at least an order of magnitude greater than on the adjacent shoals, probably due to focusing mechanisms that are poorly understood.