声波与水生生物

随着21世纪的来临,交叉学科的兴起与发展已成为科技发展的鲜明趋势,尤其是与生物学相关的交叉学科的发展更是显示出广阔的前景[1]。近年来,声波对水生生物影响与作用的研究已引起国内外生物界和声学界的重视和兴趣。本文介绍国内外一些学者研究声波对水生生物的影响与作用及其应用的若干成果。1　声波对水生生物的影响与作用1.1　超声波对鱼卵孵化的影响水生生物的特性阻抗(密度与声速的乘积)与水的特性阻抗相接近,因此,超声波对生物体活组织具有相当强的穿透力。生物体活组织能够吸收透入到它内部的超声波高频振动能量而产生出各种生物效应…     

环境RNA技术在水生生态系统生物多样性监测中的应用

随着人类活动在全球范围内的不断增强，水生生态系统的生物多样性丧失，群落结构发生巨大变化。因此，进行准确可靠的生物多样性监测来确定目标区域的物种丰度和群落结构对生态保护和资源可持续利用至关重要。环境DNA技术（eDNA）作为一种非入侵性的新手段，正在迅速发展，然而，eDNA技术易出现假阳性，从而导致实时生物多样性监测不准确的现象仍然普遍存在。与eDNA技术相比，环境RNA技术（eRNA）的快速降解使得它具有减少eDNA监测结果假阳性的潜力。本文概述了eRNA技术在水生生态系统的生物多样性监测中的可行性，主要从eRNA技术在水生生态系统中的可检测性和提高检测分辨率的潜力两个方面来介绍；综述了eRNA技术目前在水生生态系统生物多样性监测方面的研究进展；指出了eRNA技术目前存在的问题并分析了未来的研究方向。本文对未来将eRNA技术实际应用于水生生态系统的生物多样性监测、生态保护和资源可持续利用具有一定的参考价值。     

水生生物的辐射剂量率 Ⅰ.水生环境中放射性核素的浓度

存在于自然界中的天然放射性同位素和由于原子能事业发展而产生的人工放射性同位素,都能对其环境中的生物产生电离辐射效应,并能形成一定的剂量率。研究目前水生环境中放射性核素的浓度及其对水生物产生的剂量率对于保护环境和人类的健康都有一定的重要意义。由于生物体的污染直接关系到人类的健康,所以,保护生物体免受污染、制定一个生物群落和生态系保护标准的工作日益受到各国的重视。     

我国热带生物海岸的现状及生态系统的修复与重建

评述了以红树林和珊瑚礁为代表的我国热带生物海岸的分布及其面积、生物群落的破坏、海岸保护与管理、科学研究等各个方面的现状，以及生态系统修复与重建技术研究进展，指出热带生物海岸及其上的红树林、珊瑚礁热带海岸生态系统具有特殊的资源价值和生态效益，对热带海岸社会经济可持续发展的十分重要；另一方面对日益频繁的人类开发活动和自然环境变异十分敏感和脆弱，而且其直接经济价值相对较低而通常易于被忽视，随着我国自然环境变异十分敏感和脆弱，而且其直接经济价值相对较低而通常易于被忽视，随着我国社会经济近20年来的快速发展，导致热带海岸地区人口、资源、环境压力加剧，热带生物海岸红树林和珊瑚礁资源已经受到生态破坏性开发活动的极大破坏而急需保护。尽管政府采取了保护管理的各种措施，包括相关法律、自然保护区、科学研究、生态系统修复与重建的实验、研究和推广，但是总体上衰退的趋势尚未扭转，热带生物海岸在我国河口海岸生态环境变异和资源可持续利用研究中具有特别重要要的意义，应该加强我国热带生物海岸的调查、监测、评估、研究，以适应热带生物海岸和相关生态系统的保护、管理、恢复、重建和可持续发展的紧迫要求。     

紫外辐射对鱼类及水生无脊椎动物影响的研究进展

全球性的气候变化严重地威胁着自然生态环境间的平衡。在影响生态系统功能的诸多因素中,太阳紫外辐射的增强逐渐成为最受关注的全球性环境问题之一。太阳紫外辐射对地球生物的生命过程有着举足轻重的影响。生物体一旦吸收了高能量的紫外辐射,则可对其各种生理过程产生影响,打破内稳态,尤其是紫外辐射对DNA的损伤作用,是诱导一系列生物效应的主要原因。本文综述了近年来与鱼类及水生无脊椎动物有关的紫外辐射研究。从紫外辐射对生物不同发育时期的影响、生物对紫外线响应方式、紫外辐射与其他因素复合影响三个层面进行总结。通过总结紫外辐射对鱼类及水生无脊椎动物的影响,可为预测紫外辐射在水生生态系统中的作用提供助力,为海洋生物对环境变化的生理响应研究提供参考。     

P糖蛋白及其在水生生物多型异源物质抗性中的研究进展

多型异源物质抗性机制(multixenobiotic resistance mechanism,MXR)是在水生生物中广泛存在的1种对外源毒性物质的重要防御机制,其分子基础与哺乳动物细胞系的多药抗性机制非常相似,ABC(ATP binding cassette,ABC)转运蛋白超家族中的P糖蛋白(p-glycoprotein,P-gp)参与并介导了该抗性机制。本文从P糖蛋白活性和组织分布,P糖蛋白的诱导和抑制,P糖蛋白的表达和调控,多型异源物质抗性机制中P糖蛋白的可能作用模式等几个方面综述了异源物质存在条件下水生生物多型异源物质抗性机制中P糖蛋白的研究现状。大量研究表明,多型异源物质抗性机制是水生生物体的一个重要细胞防御机制。P糖蛋白作为能量依赖性的外排泵,通过水解ATP或与ATP结合将广谱的异源物质(如毒素、重金属、有机污染物等)泵出胞外或促进异源物质在特定的细胞或细胞器内滞留或隔离,从而防止异源物质与细胞内的靶分子结合,降低异源物质对细胞造成的伤害,提高生物体的抗性和耐受性。     

RNA干扰技术在水生生物研究中的应用

RNA干扰(RNA interference,RNAi)是生物体内1种抑制特定基因表达的机制,在果蝇、线虫、真菌和哺乳动物等很多类生物中存在.RNAi也存在于虾、斑马鱼、海鞘等水生生物中,斑马鱼、海鞘等的研究结果证明:RNAi技术是研究水生生物胚胎发育和基因功能的有效方法.众所周知,海水养殖生物病毒病的防治是世界性难题,利用RNAi技术对病毒的特定基因进行干扰,将是1种新型抗病毒治疗方法.为了积极推动该项技术在中国的应用,文中介绍了RNAi在国际水生生物研究中的状况,分析了应用效果与前景.     

长江水下三角洲浅表沉积层中的生物扰动构造

为查明长江水下三角洲生物扰动类型、特征和分布规律,探讨生物扰动构造的可能影响因素,使用箱式采样器获取包括三角洲前缘、前三角洲、过渡带以及正常浅海的无扰动沉积岩心,利用高分辨X-ray成像技术对浅表沉积层中的生物扰动构造进行了研究。结果表明,长江水下三角洲发育虫孔构造和挖掘构造,虫孔构造直径0．5-2mm,长度数厘米至数十厘米,多数虫孔垂直于层理发育,挖掘构造形态呈漏斗状,其内沉积物混合强烈;生物扰动构造具有明显的分带性,三角洲前缘生物扰动指数〈1,前三角洲及过渡带扰动指数为2-3级,最高达4级,而紧靠前三角洲的正常浅海生物扰动指数为2级。生物扰动构造的发育主要受上覆水体盐度、悬浮体含量制约,在盐度接近正常浅海水体盐度、悬浮体含量中等的前三角洲、过渡带是生物扰动构造发育的有利环境。研究未发现底质沉积物类型、底质环境因子对生物扰动构造的明显制约作用。     

木质素作为生物标志物的研究进展

天然环境中的木质素仅来源于陆生维管植物,不存在于海洋生物体中,且在环境介质中具有较高的化学稳定性和抗降解能力,因而是一种良好的陆源有机物的生物标志物,目前已在土壤、河流、湖泊和海洋中的地球化学研究中得到广泛应用。本文回顾了近几十年来木质素作为生物标志物的研究成果,简述了木质素分析方法,讨论了木质素在有机物溯源和重建古环境等领域中的研究进展,分析了其应用中存在的问题并对今后的研究进行了展望。     

海滨生物地貌研究进展

海滨环境孕育了各式各样的生物地貌,通过生物与环境之间的物质能量交换,海滨生态系统的生物组分与地貌组分之间双向作用得以实现。特别是在现代海岸发育进程中,生物地貌塑造者对局地地貌产生的微弱作用,可以在宏观尺度上对海滨地貌的形成和发展产生深远影响。本文简要介绍了海滨生物地貌作用的主要机制和类型、海滨环境不同生物地貌作用复杂多变的动态过程,并对研究海滨环境生物地貌作用的方法、探索生物地貌格局的多尺度成因、递归的循环过程规律以及海滨生物过程与非生物干扰及过程的相互联系等领域取得的成果进行了总结。当前,海滨生物过程和动力-沉积-地貌过程之间的双向研究已成为认识和利用生物海岸对全球变化响应和反馈作用的关键,今后还需要对不同生物地貌作用的耦合、海滨生物地貌过程的生物作用模拟以及海滨生物地貌过程的演化等问题进行深入研究。     

The effect of human use of sandy beaches on developmental stability of Talitrus saltator (Montagu, 1808) (Crustacea, Amphipoda). A study on fluctuating asymmetry

In recent years, there has been an increasing intensity of human use of coastal areas in Galicia (NW Spain). Actually, there is great concern about rapid and unplanned urban and industrial development on certain locations, as this can generate adverse impacts on those areas. In this study, we selected three sandy beaches along the Galician coast (Chanteiro, Insuela and Valieros) facing different levels of anthropic pressure, and we analysed Talitrus saltator individuals with the aim of elucidating whether anthropogenic pressures on beaches such as tourism or pollution have an influence on the incidence of morphological developmental alterations in sandhoppers in the field. Specifically, levels of fluctuating asymmetry were selected as indicators of environmental stress. Results of two sampling dates (May and September) show that individuals collected at the most touristy and polluted beach were those showing the highest asymmetry values, although results were only statistically significant for samples collected during spring. Results are in accordance with the hypothesis that beach management and pollution reduce symmetry in sandhoppers living in altered beaches.     

Sub-lethal effects of metal exposure: physiological and behavioural responses of the estuarine bivalve Macoma balthica

Variation in glycogen concentration, condition index (CI) and filtration activity were measured in the bivalve Macoma balthica buried in sediment and experimentally exposed to cadmium (Cd). The stress due to elevated but sub-lethal concentrations (300 ppb Cd) affected the overall fitness of the organism as all parameters monitored responded significantly. Lower concentrations tested (10, 30 and 100 ppb) only induced a significant decrease in filtration activity, which may play a protective role, enabling the organism to slow down its metabolic activity and preserving the integrity of its reserves (reflected by stable CI and glycogen levels). Hence, the various endpoints selected show different thresholds. Our results also demonstrate that under high exposure, small individuals loose proportionally more glycogen per unit of weight than larger ones, thus confirming the higher sensitivity of small individuals to metal contamination. Furthermore, exposure to intermediate concentration (30 ppb) seems to be beneficial to the small individuals as indicated by their high CI values compared to the control. These results showed thus that non-sigmoidal concentration-response relationship and sizes of individuals should be considered in monitoring programmes and risk assessment.     

Energy homeostasis as an integrative tool for assessing limits of environmental stress tolerance in aquatic invertebrates

Energy balance is a fundamental requirement of stress adaptation and tolerance. We explore the links between metabolism, energy balance and stress tolerance using aquatic invertebrates as an example and demonstrate that using key parameters of energy balance (aerobic scope for growth, reproduction and activity; tissue energy status; metabolic rate depression; and compensatory onset of anaerobiosis) can assist in integrating the effects of multiple stressors and their interactions and in predicting the whole-organism and population-level consequences of environmental stress. We argue that limitations of both the amount of available energy and the rates of its acquisition and metabolic conversions result in trade-offs between basal maintenance of a stressed organism and energy costs of fitness-related functions such as reproduction, development and growth and can set limit to the tolerance of a broad range of environmental stressors. The degree of stress-induced disturbance of energy balance delineates transition from moderate stress compatible with population persistence (pejus range) to extreme stress where only time-limited existence is possible (pessimum range). It also determines the predominant adaptive strategy of metabolic responses (energy compensation vs. conservation) that allows an organism to survive the disturbance. We propose that energy-related biomarkers can be used to determine the conditions when these metabolic transitions occur and thus predict ecological consequences of stress exposures. Bioenergetic considerations can also provide common denominator for integrating stress responses and predicting tolerance limits under the environmentally realistic scenarios when multiple and often variable stressors act simultaneously on an organism. Determination of bioenergetic sustainability at the organism's level (or lack thereof) has practical implications. It can help identify the habitats and/or conditions where a population can survive (even if at the cost of reduced reproduction and growth) and those that are incapable of supporting viable populations. Such an approach will assist in explaining and predicting the species' distribution limits in the face of the environmental change and informing the conservation efforts and resource management practices.     

Sub-lethal effects of metal exposure: physiological and behavioural responses of the estuarine bivalve Macoma balthica

Variation in glycogen concentration, condition index (CI) and filtration activity were measured in the bivalve Macoma balthica buried in sediment and experimentally exposed to cadmium (Cd). The stress due to elevated but sub-lethal concentrations (300 ppb Cd) affected the overall fitness of the organism as all parameters monitored responded significantly. Lower concentrations tested (10, 30 and 100 ppb) only induced a significant decrease in filtration activity, which may play a protective role, enabling the organism to slow down its metabolic activity and preserving the integrity of its reserves (reflected by stable CI and glycogen levels). Hence, the various endpoints selected show different thresholds. Our results also demonstrate that under high exposure, small individuals loose proportionally more glycogen per unit of weight than larger ones, thus confirming the higher sensitivity of small individuals to metal contamination. Furthermore, exposure to intermediate concentration (30 ppb) seems to be beneficial to the small individuals as indicated by their high CI values compared to the control. These results showed thus that non-sigmoidal concentration–response relationship and sizes of individuals should be considered in monitoring programmes and risk assessment.     

水产加工技术发展现状及展望

综述了水产品加工技术研究及其应用现状和展望，并对海洋水产资源的开发利用现状和前景作了论述，许多先进技术，如微波技术，辐照杀菌技术，超高压杀菌技术等，在水产品加工业中应用前景广阔，对海洋低值鱼类资源，水产加工废弃物，以及尚未利用的海洋生物资源的开发利用已引起重视，海洋生物技术在开发海洋食用生物资源方面具有重要意义，现代高新技术的应用将成为２１世纪水产加工业发展趋势。     

Nrf2参与水生动物氧化应激调控的研究进展

环境变化会诱导机体活性氧(reactive oxygen species, ROS)水平升高,从而产生氧化应激。氧化应激对所有生物的生存、生长、发育和进化都具有深远的影响。核因子E2相关因子2 (nuclear factor erythroid 2 related factor 2, Nrf2)被公认为细胞氧化应激调控的主导者,与伴侣蛋白Kelch样环氧氯丙烷相关蛋白1 (kelch-like ECH-associated protein 1, Keap1)一起控制数百个解毒酶和抗氧化蛋白编码基因的表达。近年来, Nrf2在水生动物中逐渐获得重视,并在一些模式鱼类如斑马鱼、鲤鱼及其他一些鱼类和水生无脊椎动物中得到研究。介绍了Nrf2的结构以及调控机制,回顾了近年来水生动物Nrf2通路参与氧化应激调控所取得的进展。研究表明, Nrf2在水生动物中广泛存在,在非生物(金属、有机污染物、无机盐、药物及微塑料等)、生物(细菌、病毒、有毒藻类)以及生境变化(冰融、盐胁迫)诱导的氧化应激调控中发挥重要作用。Nrf2一经激活入核,在小Maf蛋白的协助下与抗氧化反应元件(antioxidant-responseelement,ARE)结合,启动一系列ARE驱动基因的表达,并和孕烷X受体(pregnane X receptor, Pxr)、丝裂原活化蛋白激酶(mitogen-activated protein kinase, MAPK)以及芳烃受体(arylhydrocarbonreceptor,AhR)等细胞通路协同作用参与一系列生理过程。Nrf2在水生动物响应环境变化过程中发挥重要的细胞保护机制,有望发展成为抗逆育种潜在的基因靶点。     

水产养殖中的环境胁迫及其预防--营养学途径

环境胁迫强弱程度的不同对养殖动物的生理功能、免疫力、生长和繁殖有不同程度的负面影响。本文综述近10多年来关于水产养殖环境胁迫的最新研究成果,重点介绍如何通过营养调控提高养殖动物的抗胁迫能力,以缓解或减轻环境胁迫所带来的负面效应。并指出未来的重要研究方向是探讨提高养殖动物抗胁迫能力的途径和作用机制。     

Seasonal variation of antioxidant and biotransformation enzymes in barnacle, Balanus balanoides, and their relation with polyaromatic hydrocarbons

Seasonal variations in the antioxidant enzymes (catalase, superoxide dismutase [SOD], NADH-DT diaphorase), biotransformation enzyme, glutathione-S-transferase (GST) and microsomal lipid peroxidation in digestive tissue of barnacle, Balanus balanoides, from polluted and non-polluted populations have been evaluated. Relationships with accumulated polyaromatic hydrocarbon (PAH) concentration in barnacle tissues and environmental parameters (water temperature, salinity, dissolved oxygen concentration, water pH) were determined. As a general trend, maximum antioxidant enzyme and GST activities were detected in the pre-monsoon period or summer (March-June) followed by a gradual decrease during the monsoon (July October) with a minimum in the post-monsoon period or winter (November February). This pattern was similar to tissue concentrations of PAHs, resulting in a significant positive correlation with antioxidant enzymes, mainly catalase and SOD. Microsomal lipid peroxidation exhibited an almost reverse trend of seasonal variation to that of antioxidant enzyme activities indicating an enhanced susceptibility of barnacle tissues to oxidative stress. Among the environmental parameters, only water temperature seemed to have a significant effect on observed variations of antioxidant enzymes and GST activities. The barnacles from polluted and non-polluted populations exhibited seasonal differences in the activities of all the enzymes studied, particularly catalase, SOD and GST, suggesting the possibility of some biochemical adaptation in organisms from a chronically polluted environment. The results indicated that antioxidant defense components, catalase and SOD, are sensitive parameters that could be useful biomarkers for the evaluation of contaminated aquatic ecosystems. The results also suggested the potentiality of barnacle, B. balanoides, as a bioindicator organism against organic pollution.     

The influence of salinity on the heat-shock protein response of Potamocorbula amurensis (Bivalvia)

For biomarkers to be useful in assessing anthropogenic impacts in field studies involving aquatic organisms, they should not be affected by naturally occurring changes in environmental parameters such as salinity. This is especially important in estuarine environments and for relatively unspecific biomarkers like heat-shock proteins (hsps, stress proteins). In this study, the heat-shock protein response was measured in the euryhaline clam, Potamocorbula amurensis, after exposure to a range of salinities reflecting normal and extreme environmental conditions in Northern San Francisco Bay, California. The ability to raise cellular hsp70 levels in response to heat-shock was significantly impaired in P. amurensis collected from a low (0.5 ppt) salinity field site, and after 14 day exposure to low salinity in the laboratory.     

Seasonal variations in ectotherm growth rates: Quantifying growth as an intermittent non steady state compensatory process

Generally, growth rates of living organisms are considered to be at steady state, varying only under environmental forcing factors. For example, these rates may be described as a function of light for plants or organic food resources for animals and these could be regulated (or not) by temperature or other conditions. But, what are the consequences for an individual's growth (and also for the population growth) if growth rate variations are themselves dynamic and not steady state? For organisms presenting phases of dormancy or long periods of stress, this is a crucial question. A dynamic perspective for quantifying short-term growth was explored using the daily growth record of the scallop Pecten maximus (L.). This species is a good biological model for ectotherm growth because the shell records growth striae daily. Independently, a generic mathematical function representing the dynamics of mean daily growth rate (MDGR) was implemented to simulate a diverse set of growth patterns. Once the function was calibrated with the striae patterns, the growth rate dynamics appeared as a forced damped oscillation during the growth period having a basic periodicity during two transitory phases (mean duration 43 days) and appearing at both growth start and growth end. This phase is most likely due to the internal dynamics of energy transfer within the organism rather than to external forcing factors. After growth restart, the transitory regime represents successive phases of over-growth and regulation. This pattern corresponds to a typical representation of compensatory growth, which from an evolutionary perspective can be interpreted as an adaptive strategy to coping with a fluctuating environment.