毛竹微型桩支护浅基坑模型试验

毛竹是一种高强环保建筑材料,可以解决传统浅基坑支护成本高和后期临时结构的拆除费用等问题。开展竹管桩抗弯试验与单排微型竹桩模型试验研究,结果表明,压弯试验中随着竹桩自身净截面积的增加,其弹性模量逐渐减小,竹桩的弹性模量主要分布在8.0～12.5 GPa;模型试验中桩身应变随着埋深逐渐增加直至峰值,之后逐渐衰减为0,在到达0后出现反向应变,反向应变达到峰值后衰减为0。根据试验中桩身应变和桩顶侧移结果可知,浅基坑支护中使用单排微型竹桩能取得较好的支护效果。研究结果可为浅基坑竹桩支护作用机制和工程设计提供参考。     

Iron biomineralization by anaerobic neutrophilic iron-oxidizing bacteria

Minerals formed by bio-oxidation of ferrous iron (Fe(II)) at neutral pH, their association with bacterial ultrastructures as well as their impact on the metabolism of iron-oxidizing bacteria remain poorly understood. Here, we investigated iron biomineralization by the anaerobic nitrate-dependent iron-oxidizing bacterium Acidovorax sp. strain BoFeN1 in the presence of dissolved Fe(II) using electron microscopy and Scanning Transmission X-ray Microscopy (STXM). All detected minerals consisted mainly of amorphous iron phosphates, but based on their morphology and localization, three types of precipitates could be discriminated: (1) mineralized filaments at distance from the cells, (2) globules of 100 ± 25 nm in diameter, at the cell surface and (3) a 40-nm thick mineralized layer within the periplasm. All of those phases were shown to be intimately associated with organic molecules. Periplasmic encrustation was accompanied by an accumulation of protein moieties. In the same way, exopolysaccharides were associated with the extracellular mineralized filaments. The evolution of cell encrustation was followed by TEM over the time course of a culture: cell encrustation proceeded progressively, with rapid precipitation in the periplasm (in a few tens of minutes), followed by the formation of surface-bound globules. Moreover, we frequently observed an asymmetric mineral thickening at the cell poles. In parallel, the evolution of iron oxidation was quantified by STXM: iron both contained in the bacteria and in the extracellular precipitates reached complete oxidation within 6 days. While a progressive oxidation of Fe in the bacteria and in the medium could be observed, spatial redox (oxido-reduction state) heterogeneities were detected at the cell poles and in the extracellular precipitates after 1 day. All these findings provide new information to further the understanding of molecular processes involved in iron biomineralization by anaerobic iron-oxidizing bacteria and offer potential signatures of those metabolisms that can be looked for in the geological record.     

Kinetic tests comparison and interpretation for prediction of the Joutel tailings acid generation potential

Five sulfide mine tailings coming from the Joutel mine tailing ponds (Quebec, Canada) were tested by the humidity cell test (30 to 52 cycles duration) and the column test (11 to 12 cycles duration). The objectives of this study were twofold. First, there was the determination of the tailings acid generation potential for site reclamation. Second, there was the kinetic test comparison for understanding the tailings geochemical behavior under different test conditions. The samples used had a wide diversity in terms of acid-generation potential, particle size distribution, and parameters influencing reaction rates. Leachates produced remained at a near neutral pH for the duration of the tests. Evolution of the main elements involved in the dissolution processes demonstrated neutralization by carbonates as a response to the acid generated by sulfide oxidation. Depletion rates given by sulfates are higher for the humidity cell tests when compared to those obtained for the column tests. This is consistent with most studies to date, the humidity cell test being considered as more severe. However, by taking the ratio between cumulative elements coming from neutralization and the ones coming from oxidation, similar curves (named herein oxidation –neutralization curves) for all tests were obtained. These results show that overall geochemical behavior of the tailings is similar at near neutral pH for both types of tests. With this interpretation method, the acid-generation potential of the Joutel tailings were tested and compared to the static test results to constrain their uncertainty zone with regard to the studied tailings. The tailings geochemical behavior (carbonate dissolution response to sulfide oxidation) at near neutral pH condition appears slightly dependent of test conditions under certain hypothesis.     

岩溶热储井间连通性的示踪研究

岩溶热储储层的不均匀性强,采灌井之间的连通性不易确定。示踪技术可以将运移参数量化,有效刻画储层流体的特征,研究回灌井和开采井之间的水力联系,包括导水通道,流体流速等信息,对长期回灌可能引起的开采井的冷却进行预测,是岩溶热储井间连通性研究十分有效的技术。本文以华北牛驼镇地热田雄县地区为例,针对蓟县系雾迷山组岩溶热储,采用荧光素钠示踪剂,进行了1口井注入,10口井观测的群井示踪试验。采用裂隙介质溶质运移模型,对示踪试验数据进行了解释,得到优势通道的长度、渗透流速、纵向弥散度、回收率等储层性质,获得了试验区内采灌井之间的连通特征。对调整采灌井布局提出了建议。     

Magnetite biomineralization induced by Shewanella oneidensis

Shewanella oneidensis is a dissimilatory iron reducing bacterium capable of inducing the extracellular precipitation of magnetite. This precipitation requires a combination of passive and active mechanisms. Precipitation occurs as a consequence of active production of Fe²⁺_(aq) when bacteria utilize ferrihydrite as a terminal electron acceptor, and the pH rise probably due to the bacterial metabolism of amino acids. As for passive mechanisms, the localized concentration of Fe²⁺_(aq) and Fe³⁺_(aq) at the net negatively charged cell wall, cell structures and/or cell debris induces a local rise of supersaturation of the system with respect to magnetite, triggering the precipitation of such a phase.These biologically induced magnetites are morphologically identical to those formed inorganically in free-drift experiments (closed system; 25 °C, 1 atm total pressure), both from aqueous solutions containing Fe(ClO₄)₂, FeCl₃, NaHCO₃, NaCO₃ and NaOH, and also from sterile culture medium added with FeCl₂. However, organic material becomes incorporated in substantial amounts into the crystal structure of S. oneidensis-induced magnetites, modifying such a structure compared to that of inorganic magnetites. This structural change and the presence of organic matter are detected by Raman and FT-IR spectroscopic analyses and may be used as a biomarker to recognize the biogenic origin of natural magnetites.     

Kinetic fractionation of Fe isotopes during transport through a porous quartz-sand column

Sorption and desorption processes are an important part of biological and geochemical metallic isotope cycles. Here, we address the dynamic aspects of metallic isotopic fractionation in a theoretical and experimental study of Fe sorption and desorption during the transport of aqueous Fe(III) through a quartz-sand matrix. Transport equations describing the behavior of sorbing isotopic species in a water saturated homogeneous porous medium are presented; isotopic fractionation of the system (Δ_{sorbedmetal-soln}) being defined in terms of two parameters: (i) an equilibrium fractionation factor, α_e; and (ii) a kinetic sorption factor, α₁. These equations are applied in a numerical model that simulates the sorption-desorption of Fe isotopes during injection of a Fe(III) solution pulse into a quartz matrix at pH 0-2 and explores the effects of the kinetic and equilibrium parameters on the Fe-isotope evolution of porewater. The kinetic transport theory is applied to a series of experiments in which pulses of Na and Fe(III) chloride solutions were injected into a porous sand grain column. Fractionation factors of α_e = 1.0003 ± 0.0001 and α₁ = 0.9997 ± 0.0004 yielded the best fit between the transport model and the Fe concentration and δ⁵⁶Fe data. The equilibrium fractionation (Δ⁵⁶Fe_{sorbedFe-soln}) of 0.3‰ is comparable with values deduced for adsorption of metallic cations on iron and manganese oxide surfaces and suggests that sandstone aquifers will fractionate metallic isotopes during sorption-desorption reactions. The ability of the equilibrium fractionation factor to describe a natural system, however, depends on the proximity to equilibrium, which is determined by the relative time scales of mass transfer and chemical reaction; low fluid transport rates should produce a system that is less dependent on kinetic effects. The results of this study are applicable to Fe-isotope fractionation in clastic sediments formed in highly acidic conditions; such conditions may have existed on Mars where acidic oxidizing ground and surface waters may have been responsible for clastic sedimentation and metallic element transport.     

非饱和重塑砂土动力特性循环单剪试验研究

利用英国WFI生产的WF25735循环单剪试验系统,完成了一系列不同含水率重塑砂土在不同剪应变条件下的循环剪切试验,分析了不同剪应变条件下含水率的变化对非饱和砂土动力特性的影响规律。试验结果表明：动剪模量Gd/Gdmax-?归一化数值,在大应变下存在一个临界含水率11.2%,低于临界含水率时,Gd/Gdmax随含水率的增大而增大,高于临界含水率时,随着含水率的增大,Gd/Gdmax逐渐减小。骨架曲线存在一个相同的临界含水率,当低于该临界含水率时,随着含水率的增大,骨架曲线呈缓慢增大趋势,在含水率为11.2%处达到峰值;当高于该临界含水率时,随着含水率的增大,骨架曲线下降比较剧烈,且出现软化现象,从骨架曲线可以进一步解释Gd/Gdmax-?散点图的变化趋势。对阻尼比D/Dmax-?进行归一化数值,在较大正应力下,D/Dmax随着剪应变的增大而增大。非饱和砂土的滞回耗能随着剪应变的增大而增大;随着含水率的增大,存在一个临界含水率,滞回耗能曲线呈现先增大后下降的趋势,且临界含水率随着正应力的增大出现了偏移。     

Significance,mechanisms and environmental implications of microbial biomineralization

Microorganisms can mediate the formation of minerals by a process called biomineralization. This process offers an efficient way to sequester inorganic pollutants within relatively stable solid phases. Here we review some of the main mechanisms involved in the mediation of mineral precipitation by microorganisms. This includes supersaturation caused by metabolic activity, the triggering of nucleation by production of more or less specific organic molecules, and the impact of mineral growth. While these processes have been widely studied in the laboratory, assessment of their importance in the environment is more difficult. We illustrate this difficulty using a case study on an As-contaminated acid mine drainage located in the South of France (Carnoulès, Gard). In particular, we explore the potential relationships that might exist between microbial diversity and mineral precipitation. The present review, far from being exhaustive, highlights some recent advances in the field of biomineralogy and provides non-specialists an introduction to some of the main approaches and some questions that remain unanswered.     

基于AquiferTest的抽水试验参数计算方法分析

抽水试验是水文地质勘查中最常用的水文地质试验,通过抽水试验,可准确计算水文地质参数。本文根据在河南豫东地区进行的抽水试验,分别用AquiferTest软件计算抽水试验参数和人工求参,进而进行对比分析得出:传统的人工求参,计算结果较为准确,可靠,但因人而异,有不唯一性;用AquiferTest软件计算抽水试验参数,规范、可比性好。本文最后还对参数取值问题进行了分析对比。     

瓮安震旦系磷块岩中的生物成矿作用

生物的聚磷作用是磷块岩形成不可忽视的因素,海相磷块岩的大规模形成与生物成矿作用之间的关系十分密切。黔中地区震旦系有较厚的含磷岩层,震旦纪生物群的复苏和繁盛可能对同期磷块岩的沉积有促进作用。文章以震旦系瓮安富磷地层中抽提的有机组分为主要研究载体,采用生物标志物等技术从分子水平对其进行测试。结果表明:正构烷烃等多种分子化石的存在,暗示其生物母质源于菌藻类;正构烷烃δ(~(13)C)值变化曲线反映了南沱冰期后海洋中低等水生生物的种类相对单一。     

Microbial biomineralization processes forming modern Ca:Mg carbonate stromatolites

Modern Ca:Mg carbonate stromatolites form in association with the microbial mat in the hypersaline coastal lagoon, Lagoa Vermelha (Brazil). The stromatolites, although showing diversified fabrics characterized by thin or crude lamination and/or thrombolitic clotting, exhibit a pervasive peloidal microfabric. The peloidal texture consists of dark, micritic aggregates of very high‐Mg calcite and/or Ca dolomite formed by an iso‐oriented assemblage of sub‐micron trigonal polyhedrons and organic matter. Limpid acicular crystals of aragonite arranged in spherulites surround these aggregates. Unlike the aragonite crystals, organic matter is present consistently in the dark, micritic carbonate comprising the peloids. This organic matter is observed as sub‐micron flat and filamentous mucus‐like structures inside the interspaces of the high‐Mg calcite and Ca dolomite crystals and is interpreted as the remains of degraded extracellular polymeric substances. Moreover, many fossilized bacterial cells are associated strictly with both carbonate phases. These cells consist mainly of 0·2 to 4 μm in diameter, sub‐spherical, rod‐like and filamentous forms, isolated or in colony‐like clusters. The co‐existence of fossil extracellular polymeric substances and bacterial bodies, associated with the polyhedrons of Ca:Mg carbonate, implies that the organic matter and microbial metabolism played a fundamental role in the precipitation of the minerals that form the peloids. By contrast, the lack of extracellular polymeric substances in the aragonitic phase indicates an additional precipitation mechanism. The complex processes that induce mineral precipitation in the modern Lagoa Vermelha microbial mat appear to be recorded in the studied lithified stromatolites. Sub‐micron polyhedral crystal formation of high‐Mg calcite and/or Ca dolomite results from the coalescence of carbonate nanoglobules around degraded organic matter nuclei. Sub‐micron polyhedral crystals aggregate to form larger ovoidal crystals that constitute peloids. Subsequent precipitation of aragonitic spherulites around peloids occurs as micro‐environmental water conditions around the peloids change.     

Carbonate biomineralization induced by soil bacterium Bacillus megaterium

Biogenic carbonates spawned from microbial activities are common occurrences in soils. Here, we investigate the carbonate biomineralization mediated by the bacterium Bacillus megaterium, a dominant strain separated from a loess profile in China. Upon completing bacterial cultivation, the ensuring products are centrifuged, and the resultant supernatant and the concentrated bacterial sludge as well as the un-separated culture are added separately into a Ca-CO₃ containing solution for crystallization experiments. Results of XRD and SEM analysis indicate that calcite is the dominant mineral phase formed when the bacteria are present. When the supernatant alone is used, however, a significant portion of vaterite is also precipitated. Experimental results further reveal that the bacteria have a strong tendency to colonize the center area of the calcite {1 0  4} faces. Observed crystal morphology suggests that the bacterial colony may promote the growth normal to each individual {1 0  4} face of calcite when the cell concentration is high, but may retard it or even cause dissolution of the immediate substrate surfaces when the concentration is low. SEM images taken at earlier stages of the crystallization experiments demonstrate the nucleation of calcite on the bacterial cell walls but do not show obvious morphological changes on the nanometer- to submicron-sized nuclei. δ¹³C measurements unveil that the crystals grown in the presence of bacteria are further enriched in the heavy carbon isotope, implying that the bacterial metabolism may not be the carbon sources for the mineralization. Based upon these findings, we propose a mechanism for the B. megaterium mediated calcite mineralization and conclude that the whole process involves epi- and inter-cellular growth in the local microenvironments whose conditions may be controlled by cell sequestration and proton pumping during bacterial respiration.     

海水化学演化对生物矿化的影响综述

显生宙非骨屑碳酸盐矿物经历了文石海和方解石海的交替,主要造礁生物和沉积物生产者的骨骼矿物与非骨屑碳酸盐矿物具有同步变化的趋势。这种长期的变化趋势可以用海水化学Mg/Ca摩尔比的变化来解释。流体包裹体、同位素和微量元素等证据也证实了海水化学在地质历史中经历过剧烈的变化。虽然生物诱导矿化和生物控制矿化的相对重要性一直存在争议,但古生物地层记录和人工海水养殖实验结果都表明,海水化学演化对生物矿化有重要的影响,体现在造礁生物群落的兴衰、生物起源时对骨骼矿物类型的选择以及微生物碳酸盐岩在地质历史中的分布等。这些为研究前寒武纪海水化学演化、古气候和古环境的重建、同位素地层对比以及碳酸盐的沉积和成岩等问题提供了新的思路。     

Reverse fault slip through soft rock and sand strata by centrifuge modeling tests

The devastating damage after the 1999 Chi-Chi and 1999 Izmit earthquakes has greatly motivated soil–reverse fault interaction studies. However, most centrifuge modeling studies have employed a single homogeneous soil layer during testing, which does not represent in situ conditions. Indeed, while geological conditions vary spatially, engineering soils are often underlain by soft rocks. Therefore, four centrifuge models were developed to evaluate the effect of soft rock layers on the ground surface and subsurface deformation. Sand–cement mixtures of varying thicknesses with a uniaxial compressive strength of 0.975 MPa, simulating extremely soft rock, were overlain by pluviated sandy soil. The model thickness was 100 mm, corresponding to 8 m in the prototype scale when spun at 80 g. Every model was subjected to a vertical offset of 50 mm/4 m (0.5 H; H: total sedimentary deposit thickness) along a reverse fault with a 60° dip. The results indicate that the presence of a soft rock stratum results in the creation of a horst profile at the ground surface. Additionally, the thinner the soil layer on top of the soft rock stratum is, the longer and higher the horst created at the ground surface. Consequently, the fault deformation zone lengthens proportionally with the increasing thickness ratio of the soft rock. Furthermore, the presence of soft rock as an intermediary stratum between bedrock and soil causes the deformation zone boundary on the hanging wall side to move in the direction of fault movement.

     

Nanoscale study of As biomineralization in an acid mine drainage system

Spatial and seasonal variations of the oxidation of Fe(II) and As(III) have been previously documented in the Carnoulès (Gard, France) Acid Mine Drainage (AMD) by bulk analyses. These variations may be correlated with the variations in the activity of indigenous As(III)- and Fe(II)-oxidizing bacteria living in the As-rich Carnoulès water. The activity of these bacteria indeed plays an important role in the nature and composition of the solid phases that sequester arsenic at this site. In order to better understand the interactions of microbes with Fe and As in the Carnoulès AMD, we combined Transmission Electron Microscopy (TEM) and Scanning Transmission X-ray Microscopy (STXM) to collect near-edge X-ray absorption fine structure (NEXAFS) spectra at high spatial and energy resolution and to perform high spatial resolution imaging at the 30-50 nm scale. Spectromicroscopy was performed at the C K-edge, Fe L_2,3-edge, and As L_2,3-edge, which allowed us to locate living and/or mineralized bacterial cells and to characterize Fe and As oxidation states in the vicinity of those cells. TEM was used to image the same areas, providing higher resolution images and complementary crystallographic and compositional information through electron diffraction and EDXS analysis. This approach provides unique information on heterogeneous geochemical processes that occur in a complex microbial community in an AMD environment at the micrometer and submicrometer-scale. Bacterial cells in the Carnoulès AMD were frequently associated with mineral precipitates, and a variety of biomineralization patterns were observed. While many mineral precipitates were not associated with bacterial cells, they were associated with pervasive organic carbon. Finally, abundant biomineralized organic vesicles were observed in the Carnoulès AMD. Such vesicles may have been overlooked in highly mineralized extreme environments in the past and may represent an important component in a common biomineralization process in such environments.     

Evidence of uranium biomineralization in sandstone-hosted roll-front uranium deposits, northwestern China

We show evidence that the primary uranium minerals, uraninite and coffinite, from high-grade ore samples (U₃O₈>0.3%) in the Wuyiyi, Wuyier, and Wuyisan sandstone-hosted roll-front uranium deposits, Xinjiang, northwestern China were biogenically precipitated and psuedomorphically replace fungi and bacteria. Uranium (VI), which was the sole electron acceptor, was likely to have been enzymically reduced. Post-mortem accumulation of uranium may have also occurred through physio-chemical interaction between uranium and negatively-charged cellular sites, and inorganic adsorption or precipitation reactions. These results suggest that microorganisms may have played a key role in formation of the sandstone- or roll-type uranium deposits, which are among the most economically significant uranium deposits in the world.     

Molecular orbital modeling of aqueous organosilicon complexes: implications for silica biomineralization

Researchers recently have proposed that hypercoordinate Si-organic complexes can form in biologically relevant fluids, and they have reported the first evidence for a transient organosilicon complex generated within the life cycle of an organism (Kinrade et al., 2001b, 2002). These interpretations are based upon peak assignments of ²⁹Si NMR spectra that invoke Si-polyol (e.g., Si-sorbitol) complexes with Si in five- and six-fold coordination states. However, ab initio analyses of the proposed organosilicon structures do not reproduce the experimentally observed ²⁹Si NMR chemical shifts (Sahai and Tossell 2001, 2002 and this work). In place of the originally proposed structures, we have modeled one of the observed δ²⁹Si values with a 5-fold Si-disorbitol complex involving 5-membered ring configurations (i.e., Si-O-C-C-O). The calculated δ²⁹Si value of this new structure closely matches the observed δ²⁹Si peaks in the −100 to −102 ppm range. Likewise, ²⁹Si NMR peaks near −144 ppm were well fit by a model complex in which a 6-fold Si was complexed to three sorbitol molecules in 5-membered ring configurations. The ability to reproduce the observed NMR peaks using molecular orbital calculations provides support for the controversial role of hypercoordinate organosilicon species in the uptake and transport of silica by biological systems. The existence of such complexes in turn may explain other puzzles in Si biogeochemistry, such as the persistence of dissolved silica in concentrated biological fluids, the biofractionation of Si isotopes, and fractionation of Ge from Si.     

Acid drainage potential from coal mine wastes: environmental assessment through static and kinetic tests

The present study involved the assessment of potential generation of acid drainage from a coal mining area in India. Laboratory-based static and kinetic tests on overburden samples were conducted. Results of the static tests using acid base accounting indicate that all samples may be acid generators, and their generation capacity varied between likely, possible and low. To verify the acid generation potentiality of those samples showing a high acid drainage production in the static test, the kinetic test, using humidity cell, was conducted for a period of 15 weeks. The samples were leached with simulated rain water to mimic the chemical weathering under controlled laboratory conditions and imitate actual mine site leaching. Data obtained from chemical analysis of collected leachate were used to estimate production and reaction rates of acid generation and neutralizing capacity. Based on the kinetic test, it can be concluded that presently the neutralizing capacity of the samples is better than the oxidation capacity (acid generation). But due to the high weathering rate of carbonates, as reflected by the simulated leaching test, the neutralizing materials (carbonates) will eventually be exhausted earlier (since they showed dissolution rate) than the acid generation species (sulfates). Thus, acid drainage production is predicted from that point of time, when the neutralizing capacity has been exhausted for these mine sites.