汞离子对细基江蓠孢子萌发和生长的影响

研究了4种Hg^2＋浓度对细基江蓠果孢子和四分孢子附着、萌发和生长的影响。结果表明：与对照组相比．Hg^2＋抑制了果孢子附着却促进了四分孢子的附着，但0．0025mg／L浓度与对照组的差异不显著（P〉0．05）．各浓度间差异显著（P〈O．05）；Hg^2＋在实验浓度范围内（O．0025、0．0050、0．0075、0．0100mg／L）影响了孢子的正常萌发，使孢子不能正常分裂和生长．刺激了孢子脱落，并且使盘状体的形态发生变异；Hg^2＋不恫浓度之间也存在显著差异（P〈0．05）．并且Hg^2＋浓度越高对孢子的萌发和生长影响越大。     

细基江蓠潮间带采孢子培苗试验

江蓠是经济价值较高的一种海藻，是制造琼胶的重要原料，目前无论在国际市场或国内市场都非常畅销。其中细基江蓠(Gracilaria tenuistipitata)更是优良品种，是我国目前人工栽培的理想品种之一。它的特点是生长周期短、个体较大、含胶量高(约30％)。但种苗如何解决却是一个突出的问题。我们自1977年到1978年先后进行了潮间带采孢子培苗试验，即在潮间带筑小围堤，建立简易的苗圃，用贝壳、小石块、碎珊瑚及聚乙烯或维尼纶网片等做生长基，用洒孢子水法，使江蓠孢子附着萌发，培育成幼苗。实践证明，这种苗圃每亩浅滩所培养的江蓠幼苗，可供10亩左右的海区栽培江蓠使用。这和农业上的播种育秧、种植水稻的效果相似，可以向广大群众推广，以解决人工栽培江蓠的种苗问题。     

一种植物生长刺激素——乙烯利促进江蓠孢子萌发的试验报告

江蓠是一种经济价值较高的海藻，是目前提取琼脂的主要原料。为了发展江蓠栽培事业，我们多年来进行了江蓠人工栽培方法的探索及植物生长刺激素对生长的影响试验，都取得了一定的成果。为了促进江蓠幼苗迅速生长，达到提前出苗的目的，     

湛江内湾细基江蓠的生态研究和栽培

细基江蓠Gracilaria tenuistipitata C．F．Chang et B．M．Xia属红藻门(Rhodophycophyta)真红藻纲Florideophyceae)江蓠科(Gracilariaceae)，它和江蓠属的其它种类都是提取琼胶的主要原料，为了适应工农医疗事业日益发展的需要，今后必需大力发展江蓠栽培事业。     

细基江蓠潮间带浮筏式夹苗试验

江蓠(Gratilaria)是一种经济海藻，是制造琼胶的重要原料。细基江篱(G．tenuistipitata)是优良的栽培品种。我们在1984年春节前后，用两个月的时间，在广东省湛江港进行了潮间带浮筏式栽培细基江篱试验。收获时藻体长达80厘米以上，获得了平均亩产191公斤的干品，达每公顷2．8吨以上的高产。这种栽培方法是值得推广的。     

植物生长刺激素对细基江篱生长的影响

在原来研究的基础上,进一步用不同浓度的赤霉素、2.4-二氯苯氧乙酸、β-吲哚乙酸、丰产素、α-奈乙酸、三十烷醇和秋水仙素处理了细基江篱3小时,然后在浮箋上进行人工栽培,井和未处理的藻体进行比较。结果比对照组有了不同程度的增产,由12.1％到83.6％的并找出了各种药物处理江篱的较佳浓度。为今后在江篱生产的应用上提供了重要的科学依据。     

氮源、磷源及重金属离子对绿色巴夫藻生长的影响

研究了不同氮源、磷盐、不同氮磷比以及培养液中3种重金属离子含量对绿色巴夫藻(Pavlova viridis)生长的影响。结果表明：无机氮、有机氮共同提供氮源，以及磷酸二氢钠提供磷源，氮磷比5：1时，绿色巴夫藻生长速度最快；铅、锌、铜离子可不同程度地影响绿色巴夫藻的生长，三者的毒性依次增大，三者的96h EC50分别为25．700、7．079和1．469mg／L。     

氮源、磷源及重金属离子对绿色巴夫藻生长的影响

研究了不同氮源、磷盐、不同氮磷比以及培养液中3种重金属离子含量对绿色巴夫藻(Pavlovaviridis)生长的影响。结果表明:无机氮、有机氮共同提供氮源,以及磷酸二氢钠提供磷源,氮磷比5∶1时,绿色巴夫藻生长速度最快;铅、锌、铜离子可不同程度地影响绿色巴夫藻的生长,三者的毒性依次增大,三者的96hEC50分别为25.700、7 .079和1 469mg/L。     

不同盐度对龙虎斑生长和摄食的影响

在盐度5、10、15、20、25、30条件下,以全人工配合饵料饲喂龙虎斑[鞍带石斑鱼(Epinephelus lanceolatus♂)×棕点石斑鱼(E.fuscoguttatus♀)]60 d,研究不同盐度对龙虎斑生长和摄食的影响。结果表明,不同盐度对龙虎斑生长的影响有统计学意义(P0.05),盐度5~20组增长率、特定生长率均高于盐度25、30组,差异有统计学意义(P0.05),盐度10组的增长率最高,为55.08%,盐度15组的特定生长率最大,为2.30%/d,盐度和龙虎斑生长速率的回归方程为K=0.000 09 S 4-0.006 S 3+0.131 S 2-1.055 S+4.832(R 2=0.984);不同盐度对龙虎斑的摄食率和饵料系数影响有统计学意义(P0.05),盐度15组的摄食率最高,为1.64%/d,其次为盐度10组,为1.60%/d,饵料系数在盐度15组最低,为0.820,其次为盐度10组,为0.827。因此,龙虎斑在低盐度条件下生长比高盐度快,摄食率高,饵料利用率高,最佳生长盐度为10~15。     

Effects of salinity, light and temperature on growth rates of two species of Gracilaria (Rhodophyta)

Effects of temperature, salinity and light intensity on growth rates of Gracilaria lichenoides and G tenuistipitata var. liui Zhang et Xia were tested. Eight to ten levels of each factor were first tested separately. The best growth rate was obtained under the conditions of 32～C, 30 and 240 μmol/(m2·s) for G. lichenoides, and 24℃, 20 and 200 μmol/(m2.s) for G. tenuistipitata, respectively. Then a uniform design was used to evaluate the optimal combinations of the three factors. The best conditions for the highest daily specific growth rates (% increase in wet weight) are determined to be 31.30℃, 32.10, and 287.23 μmol/(m2·s) for G. lichenoides (16.26%/d), and 25.38℃, 21.10, and 229.07 μmol/(m2·s) for G. tenuistipitata (14.83%/d), respectively.     

Effects of temperature, algae biomass and ambient nutrient on the absorption of dissolved nitrogen and phosphate by Rhodophyte Gracilaria asiatica

Gracilaria asiatica,being highly efficient in nutrient absorption,is cultivated in sea cucumber ponds to remove nutrients such as nitrogen and phosphate.It was cultured in a laboratory simulating field conditions,and its nutrient absorption was measured to evaluate effects of environmental conditions.Ammonia nitrogen(AN),nitrate nitrogen(NN),total inorganic nitrogen(TIN),and soluble reactive phosphorus(SRP) uptake rate and removal efficiency were determined in a 4×2 factorial design experiment in water temperatures(T) at 15℃ and 25℃,algae biomass(AB) at 0.5 g/L and 1.0 g/L,total inorganic nitrogen(TIN) at 30 μmol/L and 60 μmol/L,and soluble reactive phosphorus(SRP) at 3 and 6 μmol/L.AB and ambient TIN or SRP levels significantly affected uptake rate and removal efficiency of AN,NN,TIN,and SRP(P<0.001).G.asiatica in AB of 0.5 g/L showed higher uptake rate and lower removal efficiency relative to that with AB of 1.0 g/L.Nitrogen and phosphorus uptake rate rose with increasing ambient nutrient concentrations;nutrient removal efficiency decreased at higher environmental nutrient concentrations.The algae preferred to absorb AN to NN.Uptake rates of AN,NN,and SRP were significantly affected by temperature(P<0.001);uptake rate was higher for the 25℃ group than for the 15℃ group at the initial experiment stage.Only the removal efficiency of AN and SRP showed a significant difference between the two temperature groups(P<0.01).The four factors had significant interactive effects on absorption of N and P,implying that G.asiatica has great bioremedial potential in sea cucumber culture ponds.     

Effects of salinity, light and temperature on growth rates of two species of Gracilaria （Rhodophyta）

Effects of temperature, salinity and light intensity on growth rates of Gracilaria lichenoides and G. tenuistipitata var. liui Zhang et Xia were tested. Eight to ten levels of each factor were first tested separately. The best growth rate was obtained under the conditions of 32°C, 30 and 240 μmol/(m²·s) for G. lichenoides, and 24°C, 20 and 200 μmol/(m²·s) for G. tenuistipitata, respectively. Then a uniform design was used to evaluate the optimal combinations of the three factors. The best conditions for the highest daily specific growth rates (% increase in wet weight) are determined to be 31.30°C, 32.10, and 287.23 μmol/(m²·s) for G. lichenoides (16.26%/d), and 25.38°C, 21.10, and 229.07 μmol/(m²·s) for G. tenuistipitata (14.83%/d), respectively. Supported by the 908 Special Program (908-02-04-07), the National Basic Research Program of China (973 Program, No. 2006CB400608), and K. C. Wong Magna Fund in Ningbo University     

不同形式钴对凡纳滨对虾生长和组织钴含量的影响

为研究氨基酸螯合钴对凡纳滨对虾的营养效果,在凡纳滨对虾配合饲料中添加5、10、15 mg/kg的氯化钴和蛋氨酸钴。饲养8周,4周末(饲养中期)称重,8周末称重并采集对虾肝胰脏和肌肉,测定肝胰脏和肌肉中的钴含量。结果表明:钴的添加形式和钴水平不影响对虾0~4周增重率,15 mg/kg蛋氨酸钴显著提高了对虾0~8周增重率,且两种形式的钴之间差异显著(P<0.05)。51、0 mg/kg的蛋氨酸钴和5、101、5 mg/kg的氯化钴不影响8周末对虾的增重率。特定生长率、饲料系数和蛋白质效率的变化趋势与增重率类似。钴的添加形式和钴水平不影响肌肉中的钴含量,15 mg/kg的氯化钴组肝胰脏中钴含量显著高于对照组(P<0.05),其他各组间无显著差异;蛋氨酸钴各组肝胰脏中钴含量无显著差异;钴的添加形式不影响肝胰脏中钴含量。统计结果表明,促进生长方面,两种钴之间存在显著差异,蛋氨酸钴的营养效果好于氯化钴。饲料中蛋氨酸钴的添加量为15 mg/kg时,对虾生长最快,5 mg/kg时,可满足需要。     

温度对奥尼罗非鱼仔稚鱼生长、饲料利用和消化酶活力的影响

研究了温度22、25、28、31和34℃下奥尼罗非鱼(Oreochromis niloticus×O.aureus)仔稚鱼生长、饲料利用和消化酶活力。结果表明:1)不同温度对奥尼罗非鱼仔稚鱼生长有显著影响(P<0.05),温度为31℃时,仔稚鱼生长速度和绝对增重率最高,分别为0.08cm/d和0.035g/d;温度为22℃时最低,仅0.06cm/d和0.020g/d。2)饲料系数在28℃时最低,仅1.90;22和34℃时较高,达2.14和2.17,各组差异显著(P<0.05)。3)在仔稚鱼发育和存活率上,当温度在22～34℃时,温度越高,仔稚鱼发育越好;各组仔稚鱼的存活率介于93.7%～94.7%,差异不显著(P>0.05)。4)在消化酶活力上,胃蛋白酶、胰蛋白酶和淀粉酶活力随温度升高而升高,均在34℃时活力最高;脂肪酶活力在31℃时达到最高,22℃时活力最低。