Environmental control of seed dormancy and germination in the short-lived Olimarabidopsis pumila (Brassicaceae)

The purpose of our study was to better understand seed germination ecology of the spring annual short-lived Olimarabidopsis pumila, which grows in the Gurbantonggut Desert, China. Seeds underwent after-ripening at 4, 20 ± 2 (room temperature) and 30 °C. After dormancy was broken, germination capacity was a function of temperature and presence of light. For the temperature range studied (4–30 °C), germination capacity was significantly higher between 15 and 25 °C than at temperatures above or below them. Alternating temperatures of 20/10, 25/10 and 25/15 °C were favorable for germination. Although GA₃ did promote dark germination of seeds, GA₃ did not replace light for germination. Seeds germinated to >75% if light exposure time was over 8 h and temperature and moisture conditions were favorable. Seeds were able to germinate at relatively low water potentials (83% at ?0.41 MPa), but decreasing water potentials produced detrimental effects on germination percentage and rate. Thus, dormancy characteristics and germination behavior of O. pumila seeds ensure that germination occurs in the desert only when soil moisture conditions are favorable for seedling establishment and survival.     

Effect of burial on the germination of Opuntia tomentosa's (Cactaceae,Opuntioideae) seeds

Opuntia tomentosa seeds have physiological dormancy and a permeable but hard funicular envelope that restricts water uptake and embryo growth. Dormancy break, germination, and changes in the funiculus were compared in laboratory-stored seeds and in those buried for 7 months at different microsites and later exhumed and stored in the laboratory. The funicular envelopes of both lots were examined with a scanning electron microscope (SEM). Seeds in both lots were: scarified with H₂SO₄ (0, 45, 60 and 90 min), treated with gibberellins (0, 1000 and 2000 ppm) and germinated at the same ages (0, 2, 4, 7, and 10 months after exhumation) at 24 °C. Light effect was determined at 24 °C and 20–35 °C. In laboratory-stored seeds germination was low. Exhumed seeds germinated in ～50%, the remaining ones required scarification and gibberellins, but lost dormancy two months later. Gibberellins reduced germination heterogeneity, and scarification reduced germination; the adverse effect of scarification was offset by gibberellins. Exhumed seeds germinated mainly in darkness at 20–35 °C and partially in far red light at both temperatures. SEM showed that fungi eroded the funiculus reducing its resistance. O. tomentosa could form a seed bank, ensuring its survival under heterogeneous environments.     

Seed biology of Australian arid zone species: Germination of 18 species used for rehabilitation

Revegetation of disturbed land, particularly in arid environments, is often hindered by low seedling establishment. Information on seed biology and germination cues of keystone species is lacking, particularly in arid Australia; a major zone for mining developments. This study investigated seed characteristics and germination of 18 common species required for rehabilitation of disturbed areas at Shark Bay Salt in the Shark Bay World Heritage Area, Western Australia. Untreated seeds of seven species (Aphanopetalum clematideum, Atriplex bunburyana, Austrostipa elegantissima, Melaleuca cardiophylla, Pembertonia latisquamea, Rhagodia baccata, Salsola tragus) exhibited high germination percentages. Seeds of two species (Acacia tetragonophylla, Stylobasium spathulatum) had low imbibition, which increased with hot-water treatment, hence require scarification for germination. Gibberellic acid, smoke water and karrikinolide (a butenolide isolated from smoke) substantially increased germination percentages of three species (Anthocercis littorea, Diplolaena grandiflora, Solanum orbiculatum). Seeds of the remaining six species (Dioscorea hastifolia, Eremophila oldfieldii, Nitraria billardierei, Ptilotus exaltatus, Thryptomene baeckeacea, Zygophyllum fruticulosum) had low germination percentages regardless of treatment. Most species germinated equally well at 26/13 °C and 33/18 °C, however seven species had improved performance at 26/13 °C. This study is significant to land managers and conservation agencies with an interest in optimising germination of arid zone seeds for restoration.     

Effect of environmental heterogeneity on field germination of Opuntia tomentosa (Cactaceae,Opuntioideae) seeds

We studied in Opuntia tomentosa the effect of plant shade (orientation and distance), and the place where the seeds remained before germination. Seeds were collected in 1998 and a) sown immediately on soil (non-buried seeds), b) stored in the laboratory and sown on soil during the 1999 rainy season (control) or c) buried after collection (under the shade of a tree, a rock and in an open space), exhumed 7 months later and sown in 1999 (exhumed seeds). Seeds were sown under or outside the shade of several trees; or on the northern, eastern and western sides of a 14-m-tall tree. In 1999, germination was high in all samples, but the control seeds had delayed germination. After two rainy seasons, non-buried seeds had the lowest germination rate. Germination was not affected by the distance from the tree. Outside of the plant's shade, germination was low. Germination was affected more by the environmental heterogeneity than by the individual plant shade. Germination probabilities were the highest at 16–19 °C; field germination was zero at 20.6 °C. Seed polymorphism and microenvironmental heterogeneity spread germination across two rainy seasons creating a permanent seed bank.     

Photo-thermal regulation of seed germination in natural populations of Eruca sativa Miller (Brassicaceae)

Populations of the winter annual Eruca sativa in Israel are distributed along a climatic gradient, from Mediterranean mesic habitats in the north to semi-arid and arid habitats in the south. Seed cohorts of five populations sampled across the gradient were produced in a common-garden experiment, and their germination was tested under continuous light or darkness at 5–35 °C, as well as with alternating 30/20 and 25/15 °C temperatures under a 12/12 h photoperiod. Germination was enhanced by light and by increasing temperature. Under constant temperatures maximal germination of arid and semi-arid populations was lower than in the mesic populations, suggesting on higher levels of primary dormancy with increasing aridity. Seeds that did not germinate in darkness or at low temperatures in light, germinated almost fully when exposed to GA₃ at 25 °C, indicating that imbibition under less favorable conditions induces secondary dormancy in E. sativa. Daily alternating temperatures did not affect or even reduced seed germination compared to constant 25 and 30 °C in continuous light. Exposure of dry seeds to 30 °C before imbibition increased germination to ≥80% in seed cohorts of all populations. These responses to light and temperature indicate that adaptation of E. sativa to the Mediterranean environment is associated with the ability to germinate under the relatively warm autumn temperatures, and is enhanced by exposure to high temperature during the dry summer.     

Factors controlling the regenerative cycle of Thymus funkii subsp. funkii in a semi-arid gypsum steppe: A seed bank dynamics perspective

We researched the soil seed bank dynamics of Thymus funkii subsp. funkii, a gypsovag shrub endemic to the southeastern Iberian Peninsula, to determine possible reasons for low recruitment in isolated populations growing on crusted gypsum soils. Recruitment-restriction hypotheses involving main factors in the seed bank dynamics were analysed, and a diagrammatic dynamics model drafted from a population perspective. Viable seed production averaged 362 per plant. Seed shadows resulting from primary dispersal were concentrated under the mother plant. There were two thyme-seed predators: coleopteran larvae (pre-dispersal phase) and Messor bouvieri ants (pre- and post-dispersal phases), which predated 16.4% and 3.8% of population yield, respectively. However, ants may simultaneously contribute to the spatial secondary dispersal, since they lost a small fraction of seeds they harvested. Seeds showed innate physiological dormancy to high summer temperatures, although this disappeared rapidly. The response of germination to other temperature × light conditions was wide and rapid. No permanent seed bank was detected in the soil. Seeds buried in the upper soil layer (2 cm) germinated highly and fast. In contrast, a significant fraction of seeds buried deeply (8 cm) remained viable for over one year. Germination in the field accounted for only 1.0% of the seed output, and seed death due to pathogen attack and aging was also low (3.0%). Seedling emergence was confined to autumn and showed a high correlation with total plant cover, irrespective of plant species. Seedling survival was very low. The small spatial correspondence between primary dispersal and seedling emergence patterns may reflect the importance of secondary dispersal in T. funkii seed bank dynamics. Hypotheses and consequences of such horizontal seed movements for conservation management are discussed.     

Disentangling Fabiana imbricata (Solanaceae) regeneration: The importance of disturbance and rainfall

In Mediterranean ecosystems, fire influences plant population dynamics and changes plant community structure by affecting germination and establishment of seeder shrubs. Fabiana imbricata is a long-lived seeder shrub with a broad distribution in South America. In Northwestern Patagonia grasslands there are many records of F. imbricata recruitment after fires. We hypothesized that recruitment is promoted by: 1) soil erosion that exposes deeply buried seeds; 2) direct fire effects such as heat that could break seed dormancy; 3) indirect fire effects (i.e. increase of light availability and elimination of allelopathic substances) and 4) water availability in spring. In field conditions, we tested the effects of shading and soil disturbance on F. imbricata recruitment and seed availability and distribution in the soil profile. Under controlled conditions, we investigated the influence of leaf leachate, light, heat and water on seed germination and seedling emergence. Seed germination was inhibited by leachate, and seedling emergence was inhibited by seed heated to 80 °C and 120° for 5 min. F. imbricata seedlings only emerged in germination treatments that simulated wet and very wet spring conditions. Fire and postfire wind that favor seed exposure, combined with high precipitation early in the growing season, would be necessary for successful shrub recruitment. When F. imbricata shrubland colonizes the grassland, it strongly modifies local vegetation structure, reduces species richness and increases fuel loads. F. imbricata is a key species in the ecosystem specially related to fire regime. Information on the postfire regeneration of this species would provide valuable knowledge of the changes in biomass accumulation in a fire-prone Mediterranean ecosystem.     

Comparison of respiratory and growth characteristics of two co-occurring shrubs from a cold desert,Coleogyne ramosissima (blackbrush) and Atriplex confertifolia (shadscale)

Coleogyne ramosissima Torr. (blackbrush) and Atriplex confertifolia [Torr. & Frem.] Wats. (shadscale) are cold desert shrubs from different families. Despite very different life histories they often grow in close geographic proximity in the Great Basin and the Colorado Plateau between 800 and 2000 m elevation. The purpose of this study is to compare the ecophysiology of slow growing and reproducing blackbrush with the ecophysiology of faster growing and reproducing shadscale. Metabolic heat and carbon dioxide production rates were measured on leaf tissue from wild plants and on lab-grown seedlings at temperatures from 10 to 35 °C at 5 °C intervals. Heat of combustion, ash content, and carbon and nitrogen contents were also measured. Substrate carbon conversion efficiencies and anabolic (or growth) rates were calculated from the respiration data. The growth rate of blackbrush was found to be approximately half that of shadscale because of lower respiration rate, but blackbrush begins growing earlier in the spring and can grow at higher temperatures when water is available. Blackbrush was observed to reproduce heavily when winter and spring precipitation is abundant.     

Factors influencing seed germination of Cyperus capitatus, inhabiting the moving sand dunes in southern Europe

Cyperus capitatus Vandelli (Cyperaceae) is distributed in coastal sandy habitats and mobile dunes of south Europe. Its seed germination ecology is not known, despite its potential to be used in re-vegetation projects. Laboratory experiments were conduced to assess the effects of salinity, light regime, cold stratification and burial on seed germination of this species. Overall, increasing salinity delayed germination, increased seed dormancy and mean time to germination (MTG), and reduced final germination percentage, inhibiting it completely above 1% of salinity; although it did not affect seed viability. C. capitatus seeds exhibited their greatest germination at levels between 0 and 1% in non-stratified seeds, and between 0 and 0.5% for stratified seeds. Thus, the effect of salt was greater for stratified seeds at 5 °C. Germination in light/darkness conditions was similar to that in full darkness. Finally, burial in sand of C. capitatus seeds appeared to have a significant effect on cumulative percentage of germination. Seeds buried at depths greater than 2 and 3 cm showed a lower germination success than those on sand surface or buried at shallower depths. Burial also affected the beginning and speed of seed germination.     

Titanium behavior in quartz during retrograde hydration: Occurrence of rutile exsolution and implications for metamorphic processes in the Sør Rondane Mountains,East Antarctica

In the central Sør Rondane Mountains, East Antarctica, orthopyroxene felsic gneiss (OPG) was converted to hornblende-biotite felsic gneiss (HBG) by hydration that accompanied the intrusion of pegmatite. The retrograde HBG contains exsolved rutile in quartz. The composition of orthopyroxene and clinopyroxene in OPG suggests a temperature of 840 °C (interpreted as the near-peak temperature), and the composition of hornblende and plagioclase in HBG suggests a temperature of 670–700 °C (interpreted as the temperature during hydration). Ti concentrations in quartz were measured using an electron probe micro-analyzer, and Ti-in-quartz thermometers were applied. Measured Ti concentrations were 110 ppm (equivalent to 760–820 °C) for homogeneous quartz from OPG and 35 ppm (650–700 °C) for an exsolution-free area of a quartz grain from HBG. The pre-exsolution Ti concentration in quartz from HBG was reconstructed with 100 μm beam diameter and 25 kV of accelerating voltage, giving 103 ppm, similar to the value obtained for homogeneous quartz in OPG. The temperatures obtained using a Ti-in-quartz thermometer are consistent with those estimated using other thermometers. Although analysis of the main constitute minerals in HBG yields the conditions of hydration, the reconstructed pre-exsolution Ti content in quartz within HBG yields the pre-hydration conditions. Thus, the Ti-in-quartz thermometer is a potentially powerful tool with which to identify the peak or near-peak temperature conditions, even for retrogressed metamorphic rocks.     

Seed germination of five Helianthemum species: Effect of temperature and presowing treatments

The germination behaviour of five Helianthemum species (H. almeriense, H. appeninum, H. cinereum, H. hirtum, H. squamatum) has been studied under controlled conditions. Constant 15, 20 or 25 °C and alternating 25/15 °C temperature regimes and 16/8 h light/dark photoperiod conditions were used. Presowing treatments applied were manual scarification, boiling water, hot water, dry heat and sulphuric acid. Germination values recorded were final germination percentage and germination rate expressed as days to reach 50% of the final germination percentage (T₅₀). Incubation temperature had no significant effect on final germination percentage for untreated seeds of the five Helianthemum species. However, variation due to temperature was significant for scarified seeds, with the lowest germination percentage attained at 25 °C. In all Helianthemum species studied, the highest germination percentages were obtained by manual scarification of seeds. Germination rate of scarified seeds decreased as germination temperature increased. The different presowing treatments investigated allowed some germination in some species, but none were any better than manual scarification. The high germination among most species studied, following mechanical rupture of the seed coat, shows that the mechanism of dormancy lies in the seed coat. The physical dormancy caused by impermeable seed-coat appears to be the main reason of poor germination of untreated seeds of Helianthemum species studied.     

Imbibition temperatures affect bitterbrush seed dormancy and seedling vigor

Bitterbrush first-year seedling survival is low. To determine whether imbibition temperatures affected seedling vigor, I compared post-imbibition seed weight, germination, and early growth among untreated, thiourea-treated, and cool-moist-treated seeds from three collections. Seedling axial lengths from untreated seeds averaged 28 mm among all imbibition temperatures. This compared to 31 mm from thiourea-treated seeds, 68 mm from seed held at 5°C for 14 days, and 118 mm from seeds held at 2°C for 28 days. There was no imbibition temperature for untreated or thiourea-treated seeds that compensated for the lack of a cool-moist treatment. Seed treatments or seedbed conditions which fully remove dormancy and reduce dormant-seed respiration appear likely to increase seedling survival during the first growing season.     

Germination responses of Medicago ruthenica seeds to salinity,alkalinity, and temperature

To determine the effects of and the interactions between salinity and temperature, and alkalinity and temperature, we conducted three germination tests of Medicago ruthenica (L.) Sojak cv. ‘Zhilixing’ seeds in growth chambers. In experiment 1, the seeds were placed at a range of temperatures (5, 10, 15, 20, 25, 30, 35, or 40 °C), and in experiments 2 and 3, we varied the salinity (0, 50, 100, 150, 200, or 250 mM NaCl) and alkalinity (1, 5, 10, 15, 20, or 25 mM Na₂CO₃), respectively, within three ranges of alternating temperatures (10–20, 15–25, and 20–30 °C). The seeds of M. ruthenica (L.) Sojak cv. ‘Zhilixing’ showed high percentage germination at all temperatures, except for 5 °C. Seeds germinated well at low NaCl and Na₂CO₃ concentrations under all three alternating temperature regimes. Approximately half the seeds germinated at high salinity and alkalinity at 15–25 °C, whereas the ungerminated seeds germinated when the high-saline and alkaline stresses were removed. These results suggest that M. ruthenica (L.) Sojak cv. ‘Zhilixing’ has potential utility as a forage legume in saline and alkaline environments.     

Nipa (Distichlis palmeri): A perennial grain crop for saltwater irrigation

The perennial saltgrass nipa (Distichlis palmeri, Poaceae) is endemic to northern Gulf of California tidal marshes flooded with hypersaline (38–42 g L⁻¹) seawater. Nipa was a wild harvest staple of the Cocopah people of the Río Colorado delta. We investigated the physiology, anatomy, chromosome number, and agronomic potential of nipa as a global food crop. Nipa seeds had 60–93% germination on salinities ranging from 0 to 30 g L⁻¹. Relative Growth Rates (RGR) on both flooded and aerobic conditions remained above 4% d⁻¹ up to 30 g L⁻¹, about half the RGR on freshwater. Nipa grain (caryopses) had 7–8% protein, 8% sugar and 79% total digestible carbohydrates (mostly starch) and only 2% ash and 8% fiber, equal to conventional grains in apparent nutritional value. Shoots were low in ash and sodium, and compared favorably to alfalfa forage in protein, digestible carbohydrates and energy contents. Mature female stands in the Colorado River delta produced an estimated 1.25 t ha⁻¹ of grain, but over two years in the greenhouse only partial flowering was observed. Nevertheless, D. palmeri appears to be worth developing as a perennial grain and forage crop, especially for salinized, flooded soils.     

Synergic effect of salinity and light-chilling on photosystem II photochemistry of the halophyte,Sarcocornia fruticosa

Laboratory experiments were conduced to assess the synergic effect of chilling and light on photosystem II photochemistry of the halophyte, Sarcocornia fruticosa, grown at different salinity concentrations (0, 170, 340, 510 and 1030 mM). Chlorophyll fluorescence was measured after chilling (at 5 °C in darkness) and light-chilling (at 5 °C and 700 μmol m^?2 s^?1) treatments, and after 24 h of recovery (at 20 °C and 75 μmol m^?2 s^?1). At 5 °C and 700 μmol m^?2 s^?1, plants grown with 0 and 170 mM NaCl showed the lowest F_v/F_m values, whereas quantum efficiency of PSII (Φ_PSII) was higher for plants grown at 170 and 340 mM NaCl, these results being consistent after two exposures to chilling treatments. Susceptibility to photoinhibition decreases when low temperature and high light are combined with high salinity. Therefore, populations of S. fruticosa that occur in arid environments with salinities c. 340 mM could show a higher tolerance to light-chilling.     

Light responses of Carex planostachys from various microsites in a Juniperus community

Juniperus communities are found on over 50 × 10⁶ ha in arid and semiarid habitats in southwestern North America. The drought tolerant sedge Carex planostachys occurs below the canopy in some of these communities. Cover and biomass of C. planostachys are high below the canopy and low in associated gaps. The purposes of this study were to investigate the temporal and spatial physiologic response of C. planostachys to abiotic changes, and determine it's light response characteristics from four contiguous microsites. Net photosynthesis was highest in spring when temperature was cooler and soil water higher, but low carbon uptake continued during summer drought. In addition, C. planostachys demonstrates a capacity to recover from extreme drought, despite water potential measured below ?9.0 MPa. Based on physiological light response curves and gas-exchange measurements, C. planostachys appears tolerant of shaded and full sun habitats. Light levels below the canopy were reduced compared to the gaps, but light saturation of C. planostachys did not change and net CO₂ uptake was only reduced slightly. Carbon uptake was coupled to light levels and not soil moisture. Observed differences in physiological attributes and variation in C. planostachys cover and biomass correspond to the presence or absence of the canopy. Low light compensation points, coupled with reduced respiratory demand, maximize photosynthetic gain in low light microsites. C. planostachys appears to acclimate across a range of light regimes, suggesting photosynthetic plasticity, allowing growth and survival in diverse light microhabitats. C. planostachys, tolerant of drought, appears anisohydric and demonstrates a capacity to acclimate to sun and shaded habitats, which could allow it to occur across a wider range of arid areas.     

Noble gases of the Yamato 000027 and Yamato 000097 lherzolitic shergottites from Mars

We used total melting and stepwise heating methods to measure noble gases within bulk samples from Yamato 000027 and Yamato 000097 lherzolites as well as a melt-vein sample from Yamato 000027. He and Ne are dominated by cosmogenic noble gases. The obtained cosmic-ray exposure age of 4.6 ± 1.5 Ma, an average age based on cosmogenic ³He, ²¹Ne, and ³⁸Ar for these samples, is consistent with the ages of other lherzolitic shergottites, indicating a common impact event for the ejection of lherzolites from Mars. Heavy noble gases released from the bulk samples at low temperatures were elementally fractionated terrestrial atmosphere. Martian noble-gas isotopic signatures, ⁴⁰Ar/³⁶Ar = 1900 and ¹²⁹Xe/¹³²Xe = 1.3, were observed at high temperatures (>1000 °C). The melt-vein sample released greater amounts of atmospheric Ar, Kr, and Xe at low temperatures than the bulk samples. Large amounts of Ar and Kr, as well as excess ⁴⁰Ar and ¹²⁹Xe, were evolved from the melt-vein sample at 1400 °C, and the gas shows very high ³⁶Ar/¹³²Xe (=3100) and ⁸⁴Kr/¹³²Xe (=76) values. Maximum ⁴⁰Ar/³⁶Ar and ¹²⁹Xe/¹³²Xe values of the melt sample were 1100 and 1.6, respectively, at 1600 °C. Cosmogenic Kr shows an absence of ⁸⁰Kr and ⁸²Kr produced by neutron capture on Br, which suggests a small pre-atmospheric body. Overall noble gas compositions for Y000027 and Y000097 support pairing for the Yamato 00 shergottites.     

Heliotropium europaeum only germinates following sufficient rainfall to allow reproduction

Regions with Mediterranean climates are seasonally arid and provide a niche for ephemeral species which germinate following discrete and variable rainfall during summer. These species must be able to detect when conditions are suitable for completion of their life cycle. Common heliotrope (Heliotropium europaeum) is one such species. It is considered a weed in its naturalised habitat in southern Australia as it uses resources that could be used by ensuing crops, and is toxic to livestock.We examined common heliotrope's germination responses to temperature and water potential, the effect of simulated rainfall on seedling emergence and plant growth in lysimeters.Fresh seeds of common heliotrope have relatively high optimum temperatures and water potentials for germination (around 35 °C and 0 MPa). Germination percentage of seeds extracted from the soil seed bank varies seasonally. In the soil types and climate of the study area, 24.2 mm is the minimum amount of rainfall resulting in emergence. Evapotranspiration required to reach minimal reproductive output was 19.4 mm.Common heliotrope's germination requirements have been selected to ensure that it will only germinate after sufficient rainfall to allow reproductive output. This is the mechanism through which common heliotrope detects its temporal niche.     

Patterns of seed occurrence in corsac and red fox diets in Mongolia

Corsac fox (Vulpes corsac) and red fox (Vulpes vulpes) diets often consist of small quantities of vegetation, including fruit and seeds that may represent important sources of energy and nutrition. However, details of the species consumed are few, but may provide insight into the role of foxes as seed dispersers. We analyzed scats of corsac (n = 408) and red foxes (n = 533) collected in an arid region of Mongolia from June 2006 to June 2007. We found 13 seed species in corsac scats, the most common being Allium polyrhizum, Asparagus gobicus, and Tribulus terrestris, and 12 species in red fox scats, the most common being Amygdalus pedunculata, Corispermum mongolicum, and T. terrestris. The proportion of scats with seeds varied significantly by season for both foxes. Seeds occurred most frequently during the breeding period, which corresponded with winter, and least frequently during the dispersal period in autumn for both species. We detected no differences in the proportion of scats with seeds between species seasonally. Our results suggest that fruit represents an important component of diet, especially during winter when availability of other prey items is low, and that both foxes may facilitate seed dispersal of some plant species.     

Comparative study of nitrogenase activity in different types of biological soil crusts in the Gurbantunggut Desert,Northwestern China

Biological soil crusts cover large areas of the Gurbantunggut Desert in northwestern China where they make a significant contribution to soil stability and fertility. The aim of this study was to quantify the potential nitrogen-fixing activity (NA) of different types of biological soil crusts in the Gurbantunggut Desert. The results suggest that NA (nmol C₂H₄ m^?2 h^?1) for each type of crusts was highly variable. Seasonal variation was also important, with all three types of crusts responding in a similar way to changes in environmental conditions. From March to May, NA was relatively low for all crust types. During this season, NA was 2.26 × 10³ for cyanobacterial crust followed by lichen crust (6.54 × 10²) and moss crust (6.38 × 10²). From June to October, all crust types reached their highest level of NA, especially lichen crust and moss crust (p < 0.01). The NA of cyanobacterial crust (9.81 × 10³) was higher than that of lichen crust (9.06 × 10³) and moss crust (2.03 × 10³). From November to February, when temperatures were consistently low (<0 °C), NA was at its lowest level, especially in cyanobacterial crust (4.18 × 10²) and moss crust (5.43 × 10²) (p < 0.01). Our results indicate that species composition is critical when estimating N inputs in desert ecosystems. In addition, all three types of crusts generally responded in a similar way to environmental conditions. The presence of N fixation activity in all crusts may contribute to the maintenance of fertility in sparsely vegetated areas and provide surrounding vascular plant with fixed nitrogen.