CHANGES ON EEG DURING SIMULATED SATURATION DIVING

During N2-O2 simulated saturation at a depth of 36.5 m the frequency, amplitude and index of a waves decreased and the activities of β and θ wave increased on the EEGs at rest. After the pressure was decompressed to normal, they could recover to the predive level. During the air excursion diving at depths of 60 and 70 m, the paramenters of EEG approximated to those of N2-O2 exposure. But at that time of the air excursion diving at a depth of 75 m they appeared the maximum. This showed that the changes related to the nitrogen partial pressure. There was an increase in slow waves during the simulated N2-O2 saturation. The activities of slow wave decreased on the EEGs in case of hyperventilation. The photic stimulation to the eyes caused the slow wave to disappear, and a rhythm could recover temporarily. When the photic stimulation was over the flow rhythm reappeared on the EEG. All the changes in EEG indicated that the encephalon function of the human body showed a gradual adaptation process during the     

DECOMPRESSION FROM NITROGEN OXYGEN SATURATION DIVING

Nine experiments were carried out on 39 healthy male adults for a total of 54 decompressions from N2-O2 saturation diving at the denths of 20-50 m. Excess supersaturation pressures of nitrogen dissolved in 1200 min tissue were calculated at all depths in decompression. In Exp. Ⅷ when chamber pressure was decompressed from 50 m to 35 m, a slight decompression sickness occurred, while AAAP1200 reached 7.05 m. Based on the above-mentioned analysis and calculation, the stage decompression schedules of N2-O2 saturation exposure at 36.5 m were revised.     

OBSERVATION OF HUMAN EEG AND TREMOR DURING SIMULATED He-O2 SATURATION DIVING AT A DEPTH OF 302 METRES

EEG and tremor of three divers are recorded during simulated He-O2 saturation diving at a depth of 302 m. The results ate as follows:1.During the exposure to He-O2, a decrease in a rhythm and increase in activity of 8 and wave appear in subjects' EEGs and sometimees characteristics with sleep stage I are shown in individual EEG,2.During the exposure to He-O2. the increase in the amplitude of postural tremor in 4-8 Hz and 8-13 Hz is recorded and the increase of the amplitude of 8-13 Hz is more noticeable.3. During the exposure to He-O2, all the divers suffered from fatigue, poor sleep and memory failure. These symptoms accorded with the records of the subjects' EEG and tremor.     

模拟36.5米氮氧饱和潜水26昼夜和60、70及75米空气巡潜对人体脑电图的影响

饱和潜水技术能提高水下作业效率,还可藉巡回潜水来增加水下作业深度,因此从六十年代以来在世界潜水技术先进国家中得到迅速发展,目前已成为进行潜水作业的一种重要手段.在空气潜水中,脑功能的变化早就引起人们的注意,高压氮会引起人体象酒精中毒样的麻醉症状,因此,一些水下生理和医学家们不但把脑电图作为潜水员选拔的体检指标,而且还用作潜水时健康检查和监护的重要手段.关于长期暴露于高气压环境下人体脑电图变化的研究尚属少见.本工作系统地记录了模拟36.5米氮氧饱和和潜水26昼夜各时期及进行不同深度空气巡潜时的脑电图,以探索长期暴露于高压氮氧环境对人体脑电图的影响以及适应过程中的变化规律.     

热带北太平洋西部固氮速率及固氮微生物组成

In the present study, we report N_2 fixation rate(~(15)N isotope tracer assay) and the diazotroph community structure(using the molecular method) in the western tropical North Pacific Ocean(WTNP)(13°–20°N, 120°–160°E). Our independent evidence on the basis of both in situ N_2 fixation activity and diazotroph community structure showed the dominance of unicellular N_2 fixation over majority of the WTNP surface waters during the sampling periods.Moreover, a shift in the diazotrophic composition from unicellular cyanobacteria group B-dominated to Trichodesmium spp.-dominated toward the western boundary current(Kuroshio) was also observed in 2013. We hypothesize that nutrient availability may have played a major role in regulating the biogeography of N_2 fixation.In surface waters, volumetric N_2 fixation rate(calculated by nitrogen) ranged between 0.6 and 2.6 nmol/(L·d) and averaged(1.2±0.5) nmol/(L·d), with 10 μm size fraction contributed predominantly(88%±6%) to the total rate between 135°E and 160°E. Depth-integrated N_2 fixation rate over the upper 200 m ranged between 150 μmol/(m~2·d)and 480 μmol/(m~2·d)(average(225±105) μmol/(m~2·d). N_2 fixation can account for 6.2%±3.7% of the depthintegrated primary production, suggesting that N_2 fixation is a significant N source sustaining new and export production in the WTNP. The role of N_2 fixation in biogeochemical cycling in this climate change-vulnerable region calls for further investigations.     

Comparative assessment of nitrogen fixation rate by 15N2 tracer assays in the South China Sea

Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes. The distribution, controlling factors, and flux of N₂ fixation in the global ocean remain uncertain, partly because of the lack of methodological uniformity. The 15N₂ tracer assay (the original bubble method → the 15N₂-enriched seawater method → the modified bubble method) is the mainstream method for field measurements of N₂ fixation rates (NFRs), among which the original bubble method is the most frequently used. However, accumulating evidence has suggested an underestimation of NFRs when using this method. To improve the availability of previous data, we compared NFRs measured by three 15N₂ tracer assays in the South China Sea. Our results indicate that the relationship between NFRs measured by the original bubble method and the 15N₂-enriched seawater method varies obviously with area and season, which may be influenced by incubation time, diazotrophic composition, and environmental factors. In comparison, the relationship between NFRs measured by the original bubble method and the modified bubble method is more stable, indicating that the N₂ fixation rates based on the original bubble methods may be underestimated by approximately 50%. Based on this result, we revised the flux of N₂ fixation in the South China Sea to 40 mmol/(m2·a). Our results improve the availability and comparability of literature NFR data in the South China Sea. The comparison of the 15N₂ tracer assay for NFRs measurements on a larger scale is urgently necessary over the global ocean for a more robust understanding of the role of N₂ fixation in the marine nitrogen cycle.     

Impacts of human interventions on the seasonal and nodal dynamics of the M2 and K1 tidal constituents in Lingdingyang Bay of the Zhujiang River Delta,China

Natural and human-induced changes may exert considerable impacts on the seasonal and nodal dynamics of M₂ and K₁ tidal constituents. Therefore, quantifying the influences of these factors on tidal regime changes is essential for sustainable water resources management in coastal environments. In this study, the enhanced harmonic analysis was applied to extract the seasonal variability of the M₂ and K₁ tidal amplitudes and phases at three gauging stations along Lingdingyang Bay of the Zhujiang River Delta. The seasonal dynamics in terms of tidal wave celerity and amplification/damping rate were used to quantify the impacts of human-induced estuarine morphological alterations on M₂ and K₁ tidal hydrodynamics in inner and outer Lingdingyang Bay. The results show that both tidal amplification/damping rate and wave celerity were considerably increased from the pre-anthropogenic activity period (Pre-AAP) to the post-anthropogenic activity period (Post-AAP) excepting the tidal amplification/damping rate in outer Lingdingyang Bay, and the variations in outer Lingdingyang Bay was larger than those in inner Lingdingyang Bay. The alterations in these two parameters were more significant in flood season than in dry season in both inner and outer Lingdingyang Bay. The seasonal variability of M₂ and K₁ tidal amplitudes were further quantified using a regression model accounting for the 18.61-year lunar nodal modulation, where this study observes a considerable alteration in M₂ constituent owing to human interventions. During the Post-AAP, the M₂ amplitudes at the downstream station were larger than those that would have occurred in the absence of strong human interventions, whereas the opposite was true for the upstream station, leading to a substantial decrease in tidal amplification in outer Lingdingyang Bay. However, it is opposite in inner Lingdingyang Bay. The underlying mechanism can be primarily attributed to channel deepening and narrowing caused by human interventions, that resulted in substantial enlargement of the bay volume and reduced the effective bottom friction, leading to faster wave celerity and stronger amplified waves.     

氮饥饿细基江蓠繁枝变型和孔石莼氨氮的吸收动力学特征

以氮饥饿海藻为材料,采用多瓶法和干扰法相结合的技术,测定两种海藻在不同起始浓度下不同时间间隔内的NH₄+吸收率,对不同阶段的吸收率进行非线性回归分析,结果表明它们均符合饱和吸收动力学特征.最大吸收率和半饱和常数均随着吸收时间的延长而降低.在整个吸收过程中,吸收曲线的初始斜率变化不大,这表明在低浓度下的吸收率不受短时间快吸收的影响而保持相对的稳定.同时两种海藻相比,在相应时间内的吸收动力学参数不同,孔石莼的最大吸收率、半饱和常数和初始斜率均大于细基江蓠繁枝变型.处于氮饥饿状态的两种海藻对NH₄+-N的吸收存在以下3个时相:(1)起始短期的快吸收阶段;(2)内部营养盐浓度控制的吸收阶段;(3)外界营养盐浓度控制的吸收阶段.     

灰化法磷测定中不同灰化助剂的效果比较:以焦磷酸钠作为磷标样

对Na₄P₂O₇加入不同灰化助剂经不同温度(200～550℃)灼烧后磷的回收率进行了研究.所研究的灰化助剂除文献中已深入研究过的MgSO₄和Mg(NO₃)₂外,还对MgCl₂等10余种盐试剂进行了实验.结果表明,除Mg(NO₃)₂外,MgCl₂,MgAc₂,CaCl₂,Ca(NO₃)₂等均能使Na₄P₂O₇灰化后的磷完全回收(灼烧温度不低于450℃),而加入MgSO₄在任何灼烧温度下磷的回收率均低于80%,效果不佳.因此,在利用目前已被广泛应用的由Solrzno和sharp所提出的方法去分析海水中颗粒磷和总磷或有机体中的磷时,应当用MgCl₂(或MgAc₂)作为灰化助剂来取代MgSO₄.     

Distributions and air-sea fluxes of CO2 in the summer Bering Sea

The 3rd Chinese National Arctic Research Expedition(CHINARE–Arctic III) was carried out from July to September in 2008. The partial pressure of CO2(pCO2) in the atmosphere and in surface seawater were determined in the Bering Sea during July 11–27, 2008, and a large number of seawater samples were taken for total alkalinity(TA) and total dissolved inorganic carbon(DIC) analysis. The distributions of CO2 parameters in the Bering Sea and their controlling factors were discussed. The pCO2 values in surface seawater presented a drastic variation from 148 to 563 μatm(1 μatm = 1.013 25×10-1 Pa). The lowest pCO2 values were observed near the Bering Sea shelf break while the highest pCO2 existed at the western Bering Strait. The Bering Sea generally acts as a net sink for atmospheric CO2 in summer. The air-sea CO2 fluxes in the Bering Sea shelf, slope, and basin were estimated at-9.4,-16.3, and-5.1 mmol/(m2·d), respectively. The annual uptake of CO2 was about 34 Tg C in the Bering Sea.     

海浪对北太平洋海-气二氧化碳通量的影响

利用4种海-气界面气体传输速率公式对比研究了北太平洋气体传输速率及其CO₂通量的季节变化特征。与单纯依赖风速的算法相比, 考虑波浪影响的气体传输速率和CO₂通量在空间分布和季节变化上具有明显差异。在低纬度地区(0°～30°N), 波浪参数使气体传输速率下降, 海洋对大气CO₂的吸收减少, 而在30°N以北范围内则出现新的气体传输速率高值区, 海洋对大气的吸收增加。进一步研究了黑潮延伸体区域的气候态月平均气体传输速率和CO₂通量。结果表明, 该区域气体传输速率和CO₂通量最大值分别出现于冬季和春季, 引入波浪参数后, 虽然该区域气体传输速率和CO₂通量平均值没有明显差异, 但季节变化强度显著增强。     

Preliminary results of the global ocean tide derived from HY-2A radar altimeter data

The HY-2A satellite, which is equipped with a radar altimeter and was launched on August 16, 2011, is the first Chinese marine dynamic environmental monitoring satellite. Extracting ocean tides is one of the important applications of the radar altimeter data. The radar altimeter data of the HY-2A satellite from November 1, 2011 to August 16, 2014 are used herein to extract global ocean tides. The constants representing the tidal constituents are extracted by HY-2A RA data with harmonic analysis ...     

急性CO2酸化对菲律宾蛤仔钙壳和呼吸作用的影响

基于渤海表层典型的碳酸盐系统,通过实验室密闭培养实验,分析急性CO₂酸化条件对菲律宾蛤仔(Ruditapes philippinarum) CaCO₃形成速率(G)和CO₂呼吸速率(R_C)的影响,以探讨局部海域CO₂酸化的底层海水在潮流或者风海流等因素的驱动下,脉冲式影响贝类栖息地时养殖贝类可能的响应。结果表明,菲律宾蛤仔在急性CO₂酸化条件下发生轻微的钙壳溶解和显著的呼吸抑制。CO₂酸化和菲律宾蛤仔的呼吸作用共同驱动钙壳溶解,溶解速率随Ω_文石下降而升高,G(μmol/(FWg·h))=0.14 × Ω_文石-0.49 (n=12, r=0.95, p<0.01)。活体菲律宾蛤仔钙壳保持稳定的Ω_文石临界值为3.5,而在Ω_文石=1.0的条件下,每天溶解的钙壳相当于贝壳总重的2‰。相较于钙壳溶解,Ω_文石改变对菲律宾蛤仔呼吸作用的影响更大,R_C(μmol/(FWg·h))=0.27 × Ω_文石+0.90 (n =12, r=0.82, p<0.01)。由于呼吸代谢决定了摄食等各种耗能行为的效率,因此本研究的结果表明,尽管菲律宾蛤仔可以通过摄食等自身调节机制来抵御造成钙壳溶解的环境胁迫,然而这一机制本身就可能受到酸化环境的不良影响。     

畸形波作用下双层水平板防波堤压力分布特性研究

基于物理模型试验,考虑畸形波参数、相对板宽、相对波高等影响因素,就畸形波对平顶双层水平板防波堤作用进行研究。首先对畸形波作用下双层水平板的波浪力分布特征进行了讨论,然后就最大波动压强、结构最大总垂向力与不规则波作用进行了对比分析。结果表明,畸形波作用下,双层水平板最大波动压力出现在前端迎浪区域附近,向尾端逐渐递减。双层水平板4个受力面的压力分布不同且有相位差,4个受力面的最大波动压力时间差约在0.1T_p~0.4T_p范围内变化。与不规则波作用比较,畸形波作用没有显著改变波压包络分布特征,但增大了波压包络强度值。试验范围内,就最大总力而言,两者最大总浮托力比值在1.06~2.45间变化;向下的最大总垂向力比值在1.22~2.07之间变化;就波动压力而言,其增大的幅度与畸形波参数α₁相关性最强,随α₁的增大而增大,在α₁=2.04~3.1试验范围内,畸形波作用时的最大压强比不规则波作用时可约增大20%~80%。就最大波吸力而言,两者的比值与畸形波参数α₄相关性最强,随α₄的增大而减小。在α₄=0.62~0.75试验范围内,最大波吸力强度的比值在1.61~0.87范围内变化。当α₄≤0.72时,畸形波作用时的最大波吸力大于不规则波作用时的最大波吸力;当α₄ > 0.72时则刚好相反。     

The carbonate system in the central South China Sea

The Clements of the carbonate system (HCO3- , CO32-, CO2, ·CO2 and Pco2) in the central South China Sea have been calculated by determining the pH values and total alkalinity of the seawater samples collected at 42 stations, combining simultaneously with the data of the temperatures, salinity and depths. The distributions of their characteristics have been briefly described and discussed. The saturation degrees (Ω) of calcite and aragonite in the studied area have also been evaluated. The ΩcaIc- and Ωarag. in the surface waters are about 5.8 and 3.9, respectively. The saturation depth is about 2200m for calcite and 1200m for aragonite. The lysocline in the studied area probably lies between 3000 m and 4000 m where the saturation degree of calcite is approximately 0.78.     

Experimental study on the 350 msw simulated heliox saturation-370 msw excursion diving

-The chief purpose of the research was to understand the physiological function change regularity, performance and adaptability of the human body living and working under high pressure for prolonged time.In January 1989, 4 naval divers entered the habital of NMRI's 500 msw saturation diving system after a series of adaptive diving training. The breathing mixture was helium-oxygen. After 55 h compression (including intermediate stages) the 350 msw depth was reached, where the divers lived and worked for 72 h 10 min. No sign of discomfort or significant HPNS was found in the 4 divers.The second and third day of the saturation exposure, the divers carried out 370 msw dry and wet chamber excursion diving 2 man-time each, the divers effectively carried out operational work under water, the total excursion time was 1 h each excursion dive.Saturation decompression started after 3-day storage exposure, the divers were gradually brought toward the surface 25 msw a day on the average by employing the linear stead     

自组装膜进样质谱系统及其在砂质沉积物异化硝酸盐还原研究中的应用

沉积物中的异化硝酸盐还原过程对于海洋氮循环起着至关重要的作用。基于15N标记的培养技术是目前测定沉积物异化硝酸盐还原的主要手段。准确快速测定15N标记的产物(29N2、 30N2)是量化异化硝酸盐还原各个过程速率的关键。本研究自行组装膜进样质谱系统用于29N2和30N2的测定,对其测量条件进行了优化。结果表明,进样蠕动泵进样流速0.80 mL/min,进样时间3~3.5 min,恒温槽温度20~25℃,同时铜还原炉温度在300~600℃的条件下,^29N2/^28N2和^30N2/^28N2的测试精密度分别可以控制在0.1%和1%以内,比较适合29N2和30N2的测定。利用自组装的膜进样质谱系统结合15N标记的培养技术研究了青岛石老人沙滩沉积物中的异化硝酸盐还原过程。石老人沙滩沉积物不存在将硝酸盐完全还原为氮气好氧的反硝化。厌氧铵氧化、厌氧反硝化和异化硝酸盐还原为铵(Dissimilatory Nitrate Reduction to Ammonium,DNRA)的潜在速率(以湿沉积物N计)分别为(0.05±0.01) nmol/(cm^3·h),(2.32±0.21) nmol/(cm^3·h)和(1.02±0.15) nmol/(cm^3·h)。厌氧反硝化是硝酸盐异化还原主要的贡献者,其比例接近70%,其次是DNRA,比例可达30%,而厌氧铵氧化的贡献最低,仅为1%。在N2产生过程中,主要贡献者是反硝化,厌氧铵氧化的贡献仅为2%。     

光强和二氧化碳浓度变化对浒苔幼苗生长及生理的影响

大气CO2浓度升高引起的海洋酸化如何在光变环境下影响大型海藻固碳量的问题,关系到未来海洋初级生产力的变化趋势。为研究大型海藻对CO2浓度升高和光强变化的响应,本文选取浒苔(Ulva prolifera)幼苗为实验材料,探讨其在不同光强下[80、260μmol/(m2·s)]和两种CO2浓度(正常CO2浓度:400μL/L和高CO2浓度:1 000μL/L)下的生理变化。研究发现,在正常CO2浓度、高光条件下,浒苔幼苗的生长最快,超氧化物特化酶(SOD)活性最高,而过氧化氢酶(CAT)活性在低光、高CO2处理下有最大值。光合色素含量和光系统Ⅱ的光化学效率在不同处理间没有显著性差异,但叶绿素a与类胡萝卜素的比值在低光正常CO2处理下有最大值。同时,高光高CO2处理下,浒苔幼苗的可溶性蛋白含量最低。     

PHYSIOLOGICAL FUNCTION AND DECOMMISSION DURING LONG-TERM N2-O2 SATURATION DIVING

The present study aims; at (1) examining the effect of prolonged exposure to high pressure on physiological functions: (2) observing performance capacity and adaptive process of the organism, and (3) evaluating the validity of saturation decompression. The results obtained from 7 subjects show that they had good adaptive capacity and could undertake moderate work load. The uniform saturation decompression scheme used and no-decompression ait excursion depth-lime limit were proved to be safe. Up to now, only a few experiments of long-term exposure to 4.65 ATA nitrox atmosphere have been reported throughout the world, so the data accumulated have Jtt reference valuc.     

CO2 exchange at air-sea interface in the Huanghai Sea

INTRODUCTIONTheroleoftheoceaniscrucialintheoverallcycleofCOZ,withitsspecialpumpingmechanismssuchassolubilitypumpingattheair-seainterfacewithcarbonatechemistry,biologicalpumpinginsurfacewatersandalsointhewatercolumn,anddynamicpumpingassociatedwithoceancirculation(BroeckerandPeng,1982).Inordertounderstandthesevariouspumpingprocessesintheocean,muchresearchhasbeencarriedoutonaglobalscaleasapartofeffortstounderstandtheglobalgeochemicalcycleofCOZ.TheHuanghaiSea,atypicalmid-latitudeepicontine…