Molecular staging of marine medaka: A model organism for marine ecotoxicity study

Oryzias melastigma, also called O. dancena, is becoming a very useful model for estuarine and marine ecotoxicity studies. With O. melastigma being adopted by ILSI Health and Environmental Science Institute (HESI) for embryo toxicity testing, improved knowledge of biomarker based embryonic development becomes especially important for mechanism-based toxicity evaluations. Using whole mount in situ hybridization and immunostaining techniques together with widely used molecular markers, this study describes the molecular development of marine medaka embryos, focusing on the brain, eye, heart, pectoral fin, pancreas, liver, muscle and neuron system. These organs are targets of environmental pollutants that disrupt normal embryonic development in medaka and other fish.     

The effects of a copper-based antifouling paint on mortality and enzymatic activity of a non-target marine organism

Antifouling paints are used on a wide range of underwater structures in order to protect them from the development of fouling organisms. The leaching of the toxic substances from the matrix of the paint causes toxic effects not only to the fouling organisms but also on other "non-target" biota. The present study addresses the impact of the antifouling paint Flexgard VI-II on brine shrimp nauplii selected as convenient test organisms. The surface to volume (S/V) concept developed by Persoone and Castritsi-Catharios (1989) was used to determine S/V-LC50s for the test biota exposed to PVC test panels of 400-1000 mm2 surface coated with the antifouling paint in test vessels containing 20 ml seawater. Total ATPase and Mg2+-ATPase were also analyzed for coated surface areas inducing less than 50% mortality in the brine shrimp nauplii. The calculated S/V-LC50 (24 h) was 24.6 mm2/ml, which shows the high toxic character of the antifouling paint. Decreased enzymatic activities were noted in the brine shrimp nauplii exposed to test panels of 50 and 100 mm2 in 20 ml seawater. The present study indicates that the "surface to volume" concept is an interesting methodology that can be applied with both lethal and sublethal effect criteria for the determination of toxic stress from leaches of painted surfaces.     

Comparative embryotoxicity of phenanthrene and alkyl-phenanthrene to marine medaka (Oryzias melastigma)

Alkylated polycyclic aromatic hydrocarbons (alkyl-PAHs) are the predominant form of PAHs in oil, comprising 85–95% of total PAHs. However, little attention has been paid to these chemicals in ecological risk assessment of marine oil spill. A comparative study of the toxic effects of phenanthrene and retene (7-isopropyl-1-methylphenanthrene, an alkyl-phenanthrene) on the early life stage of marine medaka (Oryzias melastigma) was conducted. Results showed that retene was significantly more toxic than phenanthrene, and marine medaka could be more sensitive to retene than some freshwater fishes. Retene had a higher excretion rate than phenanthrene during the larvae stage. Both of compounds resulted in developmental malformation of marine medaka embryos, with phenanthrene affecting on peripheral vascular system and yolk sac, while retene affecting on cardiac tissues. The toxicity of phenanthrene might be mainly related to its anesthetic effects, and that of retene might be related to the CYP1A-mediated toxicity of its metabolites.     

Molecular and organism biomarkers of copper pollution in the ascidian Pseudodistoma crucigaster

We studied the effects of pollution in the colonial ascidian Pseudodistoma crucigaster at organismal and suborganismal levels. Our goal was to find early biomarkers to detect some effect of pollution before changes in community structure or species composition occur. We examined the effect of Cu on the production of heat-shock proteins, defence metabolites, growth rates and presence of resistance forms. We performed a transplant experiment to a Cu polluted harbour and observed negative growth and presence of resistance forms but not depressed production of toxic metabolites or an increase in stress proteins (hsp) in the ascidian. In a laboratory experiment, stress proteins were induced only under half the Cu concentration found in the harbour. We conclude that hsp can be used in this ascidian as an early warning system for sublethal pollution but that the response is inhibited above a threshold of the stressing agent, which may vary among species.     

iTRAQ-based proteomic profiling of the marine medaka (Oryzias melastigma) gonad exposed to BDE-47

A recent study demonstrated that 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) may have an adverse effect on the reproduction in marine medaka (Oryzias melastigma), but the molecular mechanisms remain largely unknown. In this study, we investigated the protein expression profiles of male and female gonads of O. melastigma exposed to dietary BDE-47 at two dosages (0.65 and 1.30 μg/g/day, respectively) for 21 days. Extracted proteins were labeled with iTRAQ and analyzed on a MALDI TOF/TOF analyzer, as results, 133 and 144 unique proteins were identified in testis and ovary, respective, and they exerted dose- and sex-dependent expression patterns. In testis, among the 42 differentially expressed proteins; down-regulation of histone variants and parvalbumins implicated BDE-47 may disrupt the spermatogenesis and induce sterility in fishes. In ovary, 38 proteins were differentially expressed; the elevation of vitellogenins and apolipoprotein A–I expression indicated BDE-47 acts as an estrogen-mimicking compound and led to reproductive impairment in O. melastigma.     

Adriatic coast as a microcosm for global genotoxic marine contamination--a long-term field study

Global changes in the marine environment and the continuing disposal of genotoxic xenobiotics are increasing the importance of environmental pollution monitoring and of biomonitoring programs. Current approaches focus on investigations at regional and local levels in an attempt to precisely define the nature and extent of any potential environmental crisis. We have initiated, for the first time, a long-term biomonitoring program focusing on the Croatian coast of the Adriatic Sea to contribute to a more detailed understanding of marine genotoxic effects using the mussels Mytilus galloprovincialis Lam., collected along the eastern Adriatic coast over a period of five years (1998-2002), as a key test organism. The integrity of DNA in its gill homogenate was examined by the Fast Micromethod. The strand scission factor (SSF) values, as a measure of DNA integrity, DNA damage or incomplete repair have been used for the ranking of sampling sites with respect to significant genotoxic stress due to the influence or effects of genotoxic xenobiotics. The region of Split (Kastela Bay) proved to be the area with the heaviest load of genotoxic agents. The investigation of harmful effects in the ecosystem based on biomonitoring of genetic and other agents, not only on local levels but also on a wider scale, is considered as an important step in marine environmental management.     

Combining regression and spatial proximity for catchment model regionalization: a comparative study

Abstract

Spatial error regression is employed to regionalize the parameters of a rainfall–runoff model. The approach combines regression on physiographic watershed characteristics with a spatial proximity technique that describes the spatial dependence of model parameters. The methodology is tested for the monthly abcd model at a network of gauges in southeast United States and compared against simpler regression and spatial proximity approaches. Unlike other comparative regionalization studies that only evaluate the skill of regionalized streamflow predictions in ungauged catchments, this study also examines the fit between regionalized parameters and their optimal (i.e. calibrated) values. Interestingly, the spatial error model produces parameter estimates that better resemble the optimal parameters than either of the simpler methods, but the spatial proximity method still yields better hydrologic simulations. The analysis suggests that the superior streamflow predictions of spatial proximity result from its ability to better preserve correlations between compensatory hydrological parameters.

Editor D. Koutsoyiannis; Associate editor Y. Gyasi-Agyei     

Seismoelectric and electroseismic responses to a point source in a marine stratified model

We investigate the seismoelectric/electroseismic wavefields excited by a point source in an air/seawater/three-layered porous medium configuration containing a hydrocarbon layer. The results show that if an explosive source for excitation is used, receivers at seafloor can record the coseismic electromagnetic fields accompanying the P, S, fluid acoustic waves and the interface responses converted from the acoustic waves at seafloor interface and from the seismic waves at the interfaces beneath the seafloor. Employing a vertical electric dipole source shows that, with the exception of the interface responses converted from electromagnetic waves at seafloor, the interface responses converted from transmitted electromagnetic waves at the interfaces beneath the seafloor can also be identified. Given that the strength of the explosive source is within excitation capability of industry air guns, the generated interface responses from the hydrocarbon layer can be detected by current electromagnetic sensors considering the low ambient noise at the seafloor. Our results demonstrate the feasibility of the seismoelectric method applied to marine hydrocarbon exploration. Electroseismic modelling results suggest that it is not practical to employ this method to prospect marine hydrocarbon layer due to the weak interface response signal, unless a much larger current is injected into seafloor.     

Method for generating parameterized ecotoxicity data of dispersed oil for use in environmental modelling

The aim of the work was to establish methodology for realistic laboratory-based test exposures of organisms to oil dispersions, specifically designed to generate parameterized toxicity data. Such data are needed to improve the value of numerical models used to predict fate and effects of oil spills and different oil spill responses. A method for continuous and predictable in-line production of oil dispersions with defined size distribution of different oil qualities was successfully established. The system enables simultaneous comparison between the effects of different concentrations of dispersion and their corresponding equilibrium water soluble fractions. Thus, net effects of the oil droplet fraction may be estimated. The method provides data for comparing the toxicity of oil dispersions generated both mechanically and with the use of chemical dispersions, incorporating the toxicity of both dissolved oil and droplets of oil.     

A stochastic model for optimal operation of inter-basin water allocation systems: a case study

This paper presents a new methodology for optimal operation of inter-basin water transfer systems by conjunctive use of surface water resources in water donor basin and groundwater resources in water receiving basin. To incorporate the streamflow uncertainty, an integrated stochastic dynamic programming (ISDP) model is developed. In the ISDP, the monthly inflow to the reservoir in the water donor basin, the water storage of the reservoir, and the water storage of the aquifer in the water receiving basin are considered as state variables. A water allocation optimization model is embedded in the main structure of ISDP and a new ensemble streamflow prediction model based on K-nearest-neighbourhood algorithm is also developed and linked to the ISDP. By using a new reoptimization process, the ISDP model provides monthly policies for water allocation to users in water donor and receiving basins. As water users can form a coalition to increase their benefits, several solution concepts in cooperative game theory, namely Nash–Harsanyi, Shapley, Nucleolus, Weak Nucleolus, Proportional Nucleolus, Separable Costs Remaining Benefits (SCRBs) and Minimum Costs Remaining Savings are utilized to determine the profit of each water user. In the last step, stakeholders make negotiation over these solution concepts using the Fallback bargaining theory to reach a unanimous agreement on the final distribution of the total benefit. The methodology is applied to an inter-basin water transfer project and the results show that the Shapley and SCRB solutions concepts can provide better distributions for the total benefit and the total benefit of water users is increased by a factor of 1.6 when they participate in a grand coalition.     

The European Commission proposal for a marine strategy: lacking substance

The European Commission's proposed Marine Strategy constitutes a highly inadequate approach to long-term protection of the European Seas. The main problem with the strategy is the restriction to a proposed directive in which only EU Member States are placed under obligation to develop their own environmental objectives and marine protection activity programmes. This 'renationalisation' results in the exclusion of key policy areas like Common Agricultural Policy (CAP) and Common Fishery Policy in which the EU has centralised powers. Furthermore, there is no plan to refine EU environmental law relevant to marine protection and nor are there any provisions for the linking of EU-level action with the international conventions for the protection of the oceans.     

Application of artificial mussels (AMs) under South African marine conditions: a validation study

Over the last three decades there has been a significant decline in the number of marine pollution monitoring-related studies in South Africa. Thus, the current study was conducted to assess the current state of metal contamination within the South African marine environment through the validation of the artificial mussel (AM). Indigenous reference mussels (Perna perna) were deployed alongside the passive device within the South African marine environment for a 6 week exposure period. Analysis of metal uptake (Cd, Cr, Cu, Pb and Zn) was determined by filtration and elution of the AM chelex resins, microwave digestion of the transplanted mussels, and determination of their metal concentrations by ICP-MS and ICP-OES analysis. Uptake patterns between the AM and transplanted mussels showed significant temporal and spatial correlation for the majority of the elements analysed. While the AM provided relevant and complementary information on the dissolved metal concentrations, limitations were also observed.     

Coast effect distortion of marine magnetotelluric data: Insights from a pilot study offshore northeastern Japan

We report on strong coast effect distortions observed for broadband marine magnetotelluric (MT) data collected on the forearc offshore northeastern Japan. Eight days of horizontal electric and magnetic fields recorded at eight seafloor stations and the horizontal magnetic fields from a land remote station were processed with a robust multiple-station algorithm, yielding good MT responses and inter-station transfer functions at periods of 7–10,000 s. Transverse electric (TE) mode responses have cusps in apparent resistivity and negative phases at periods around 1000 s, while the transverse magnetic (TM) mode responses are galvanically depressed below the TE responses. An analysis of inter-station transfer functions confirms that the apparent resistivity cusps are a magnetic field, rather than electric field, phenomenon, consisting of an amplitude minimum and rapid phase change around a characteristic frequency. Poynting vectors for a TE coast effect model study illustrate that the anomalous phases are associated with energy diffusing back up to the seafloor from below, after being turned around from its usual downward propagating trajectory by inductive coupling between the conductive ocean and the resistive seafloor along the continental margin. We show that the characteristic frequency and position of the TE mode apparent resistivity cusps are determined by a relatively simple combination of the electrical resistivity of the seafloor, the depth of the ocean, and the distance from the coastline. By including coastlines and bathymetry in 2D inversion, we recover the seafloor conductivity structure along the forearc, demonstrating that broadband data can constrain the thickness of conductive forearc sediments and the underlying high resistivity associated with the mantle wedge and subducting oceanic lithosphere.     

The mussel Mytilus edulis as a bio-assay organism for mercury in seawater

Surveys of natural populations of mussels have identified areas of mercury contamination in the Firth of Forth, Scotland. A field bio-assay technique has been devised which accurately reflects the mean total mercury concentration in the surrounding seawater. The detection limit of the technique is estimated at 5–20 ng Hg l.^?1, and consequently the method can detect comparatively small enhancements over background mercury concentrations in estuarine and sea water.