Simplified technique for the rapid determination of phytoplankton pigments by reverse-phase high-performance liquid chromatography

A simplified technique for the rapid analysis of photosynthetic pigments in marine phytoplankton is described. The pigments extracted in N,N-dimethylformamide (DMF) are injected directly into the reverse-phase high-performance liquid chromatograph fitted with a photodiode array detector. We developed a new stepwise program; that is methanol-water (7525 v/v), methanol, methanol-acetone (8020) and methanol-acetone (6535). This program gives a good separation of at least 13 carotenoids, and 10 chlorophylls and their derivatives, completed within 20 min. The quantitative precision is high; the maximum standard error was less than 6%. The stability of pigments in DMF were also considered for application of this technique to the lab work on board the ship.     

Dynamics of the Kuroshio large meander

The bimodality of the Kuroshio path is studied numerically with a barotropic inflow-outflow model. The dynamics that determines the path depends on the Rossby number,Ro (proportional to inlet velocity) and the Reynolds number (representing effects of viscosity). At lowRo (<Ro ₁) only a meander path occurs, while at highRo(Ro ₂) only a straight path is developed. Between these critical values (Ro ₁RoRo₂) either of the two paths can occur (multiple states), and the choice of path is determined by its history. Increase (decrease) inRo acrossRo ₂ (Ro ₁) leads to catastrophic transition from one path to the other. In the intermediate range (Ro ₁RoRo₂), the straight path is conditionally unstable to finite amplitude disturbances, and abrupt changes to the meander path take place. Absolute vorticity is almost conserved along the meander path, while along the straight path it is dissipated in large amount near the coast. At low Re, the flow tends to a viscous flow, and steady states are obtained. At highRe, time variations with different periods for the meander and straight paths become dominant. Intermittent transitions from one state to the other without any changes of external parameters are found at intermediateRo and at highRe.     

Variability of the axial morphology and of the gravity structure along the Central Spreading Ridge (North Fiji Basin): evidence for contrasting thermal regimes

The Central Spreading Ridge (CSR) is located in the central part of the North Fiji Basin, a complex back-arc basin created 12 Ma ago between the Pacific and Indo-Australian plates. The 3.5 Ma old CSR is the best developed, for both structure and magmatism, of all the spreading centers identified in the basin, and may be one of the largest spreading systems of the west Pacific back-arc basins. It is more than 800 km long and 50–60 km wide, and has been intensively explored during the French-Japanese STARMER project (1987–1991).The CSR is segmented into three first order segments named, from north to south, N160°, N15° and N-S according to their orientation. This segmentation pattern is similar to that found at mid-ocean ridges. The calculated spreading rate is intermediate and ranges from 83 mm/yr at 20°30 S to 50 mm/yr at 17°S. In addition, there is a change in the axial ridge morphology and gravity structure between the northern and southern sections of the CSR. The axial morphology changes from a deep rift valley (N160° segment), to a dome split by an axial graben (N15° segment) and to a rectangular flat top high (N-S segment). The Mantle Bouguer Anomalies obtained on the northern part of the CSR (N160°/N15° segments) show bull's eye structures associated with mantle upwelling at the 16°50S triple junction and also in the middle of the segments. The Mantle Bouguer Anomalies of the southern part of the ridge (N-S segment) are more homogeneous and consistent with the observed smooth topography associated with axial isostatic compensation.At these intermediate spreading rates the contrast in bathymetry and gravity structure between the segments may reflect differences in heat supply. We suggest that the N160° and N15° segments are cold with respect to the hot N-S segment. We use a non-steady-state thermal model to test this hypothesis. In this model, the accretion is simulated as a nearly steady-state seafloor spreading upon which are superimposed periodic thermal inputs. With the measured spreading rate of 50 mm/yr, a cooling cycle of 200,000 yr develops a thermal state that permits to explain the axial morphology and gravity structure observed on the N160° segment. A spreading rate of 83 mm/yr and a cooling cycle of 120,000 yr would generate the optimal thermal structure to explain the characteristics of the N-S segment. The boundaries between the hot N-S segment and its cold bounding segments are the 18°10 S and 20°30 S propagating rifts. A heat propagation event along the N-S segment at the expense of the adjacent colder failing segments, can explain the sharp changes in the observed morphology and structure between the segments.     

Moored instrument observations in the Kuroshio south of Kyushu

In each of the (fiscal) years 1979–1982, multilayer current measurements by means of moored instruments were carried out in the Kuroshio off Tanegashima Island, south of Kyushu, with particular interest in the baroclinic layer (0–1,000 m) of the intense current. Most of the moored instruments were in position for more than 30 days, the maximum duration being 145 days. This paper describes some noticeable features of the mean and fluctuation fields in the surface Kuroshio as inferred from the the new data sets.Vertical profiles of horizontal velocities show that the eastward current of the Kuroshio in the region is confined to the upper 600 m on average and below this level the mean current is directed to the southwest along the local isobaths. This is believed to be the first observation of the level of no motion in this region. It is argued that the indicated level of no motion would lead to much smaller values for the Kuroshio transport than previous values referred to the 1,000 m level — for example, earlier estimates by Nitani (1972).The fluctuation field in the upper intense current appears to be dominated by an organized motion with a time scale of about 30 days. This can be seen clearly in the time variations of water temperature and local volume transport, but less clearly in the velocity records. The horizontal transfer components of momentum and heat are calculated and it is suggested that the transverse momentum transfer, UV tends to be negative (offshore) in the region of anticyclonic shear of the upper Kuroshio; no evidence for a negative eddy viscosity effect is found.     

Internal wave dispersion in deep oceans calculated by means of two-variable expansion techniques

An analytical study is made of linearized internal wave dispersion relation in a density-stratified deep ocean with aN(z) profile which contains turning points. Principal emphasis is placed on obtaining the-k relationship for all frequencies. Taking advantage of the largeness of the nondimensional wavenumber (kS1), the mathematical tool to attack the governing Sturm-Liouville equation is a combination of the two-variable expansion (valid far away from the turning point) and the limit-process expansion (valid in the neighborhood of the turning point). The matching conditions for these two expansion schemes provide the desired-k relationship. A numerical example of this method demonstrates satisfactory agreement with the results obtained by the strict analytical solution and the multi-layered matrix numerical model.     

Transmission and reflection of planetary and topographic Rossby waves in a two-layer ocean

Transmission and reflection problems when kissing≓ occurs among planetary and topographic Rossby waves in a two-layer ocean are studied. The slope parameterS(=dh ₂/dx, whereh ₂is the thickness of the lower layer) is assumed to have constant values in the regionsx 0 andxL and to vary linearly with the increase ofx in the region0xL (refer to Fig. 2 in the text). Furthermore, a wave is entered fromx=– and kissing is assumed to occur in the region (0<)x _axx_b(<L).It is found that a wave of the same type as the incident wave is mainly transmitted when the width of the region in which kissing occurs,L _kiss(=tx _b–x_a), is smaller than _kiss=2/(¦K¦+ _y/2), whereK is a representative wavenumber in the regionx _ab, _y is they-component of , and is the frequency. WhenL _kiss is larger than _kiss, on the other hand, the main wave transmitted is of a different type to the incident wave. As an application, transmission and reflection problems of planetary Rossby waves are considered, and it is shown that when an external (internal) planetary Rossby wave is entered, an internal (external) one can be transmitted due to the effect of kissing.     

A detailed geochemical and rock magnetic study of dredged basalt from the Sheba Ridge,Gulf of Aden

We report the results of petrological, geochemical and rock magnetic studies of basalt dredged from the eastern end of the west Sheba Ridge during cruise 11/1979 of R. R. S. Shackleton to the Western Gulf of Aden. The ridge is part of the Red Sea-Gulf of Aden spreading axis and the basalts are olivine tholeiites. The abundances of some elements are characteristic of normal MORB (mid-ocean ridge basalt) but other elemental abundances suggest affinites with transitional-type MORB.The observed magnetic properties are interpreted in terms of the composition, concentration and microstructure of the magnetic mineral fraction by recourse to the available data on synthetic analogues. The analysis has been carried out in greater detail than has been attempted in previous magneto-petrological studies. It appears that submarine weathering of the magnetic minerals (maghemitization) brings about not only the expected change in composition but also a fall in concentration of the magnetic fraction. This could result from the removal-of-iron oxidation mechanism operating in the submarine environment. It is also found that the fall in remanence with increasing degree of maghemitization is not explicable in terms of the change in composition and concentration of the magnetic minerals. A further influence—probably microstructural change—significantly reduces the remanence intensity.     

High inside corners at ridge-transform intersections

A large topographic high commonly occurs near the intersection of a rifted spreading center and a transform fault. The high occurs at the inside of the 90° bend in the plate boundary, and is called the high inside corner, while the area across the spreading center, the outside corner, is often anomalously low. To better understand the origin of this topographic asymmetry, we examine topographic maps of 53 ridge-transform intersections. We conclude the following: (1) High inside corners occur at 41 out of 42 ridge-transform intersections at slow spreading ridges, and thus should be considered characteristic and persistent features of rifted slow spreading ridges. They are conspicuously absent at fast spreading ridges or at spreading centers that lack a rift valley. (2) High inside corners occur wherever an axial rift valley is present, and an approximate 1:1 correlation exists between the relief of the rift valley and the magnitude of the asymmetry. (3) Large high inside corners occur at both long and short transform offsets. (4) High inside corners at long offsets decay off-axis faster than predicted by the square root of age cooling model, precluding a thermalisostatic origin, but consistent with dynamic or flexural uplift models.These observations support the existing hypothesis that the asymmetry is due to the contrast in lithospheric coupling that occurs in the active transform versus the inactive fracture zone. Active faulting in the transform breaks the lithosphere along a high angle fault, permitting vertical movement of the inside corner block, whereas the inactive fracture zone forms a weld that couples the outside corner to the adjacent block, preventing it from rising. Large asymmetry at very short transform offsets appears to be caused by the added effect of a second uplift mechanism. Young lithosphere in the rift valley couples to the older plate, and when it leaves the rift valley it lifts the older plate with it. At very short offsets, this coupled uplift acts upon the high inside corner; at long offsets, it may upwarp the older plate or its expression may be muted.     

Error of digitization of multidimensional random hydrophysical fields with power spectra

Exact and approximate formulae are proposed to evaluate the errors of spatial/temporal digitization (aliasing) of then-dimensional random fields withm-power spectra atn=1, , 4 and 1m5, which satisfactorily approximate temperature, velocity, density, and other fields in the sea and atmosphere.Translated by Vladimir A. Puchkin.     

Seasonal variability and the types of currents in the upper layer of the Black Sea

Spectral analysis of the climatic circulation in the upper 10 m layer of the Black Sea has been carried out. The current field was calculated from a three-dimensional baroclinic non-linear model. The mode composition of the circulation was derived. It is shown that the surface climatic circulation can be represented as the superposition of a finite number of elementary currents of two types:n- andm-modes. Only 4n-modes are shown to be energy-dominant throughout the year. A relation is established between the different sources of energy supply of currents and the modes which assimilate this energy. Problems concerning the nature of the classical Black Sea currents such as the main Black Sea current (MBSC) and the Knipovich spectacles are discussed. A scenario of the field large-scale experiment is suggested which allows the reconstruction of the amplitudes of the first 10n- and the first 10m-modes from mooring data.Translated by Mikhail M. Trufanov.     

Magnetic anomalies associated with the southeastern continental margin of South Africa

A total magnetic intensity, iso-magnetic map is presented and discussed. Between East London and Durban large east-west trending anomalies are known on land and can be traced onto the continental shelf but not beyond the slope. Elsewhere the continental shelf is characterized by a remarkably quiet magnetic field. A feature of the map is the linear anomaly, named the Cape Slope Anomaly, which is parallel to the continental margin and coincides approximately with the 68° small circle about the early pole of opening for the South Atlantic as given by Le Pichon and Hayes (1971). The anomaly is traced between 30°54S, 30°48E and 37°45S, 20°31E and is interpreted as occurring over the truncated edge of a semi-infinite, sub-horizontal, remanently magnetized plate in oceanic crust beyond the continental margin.Between 37°03S, 21°49E and 37°41S, 21°12E the Slope Anomaly occurs over a ridge named the Agulhas Ridge. A continuous seismic reflection profile over the ridge shows acoustic basement occurring under a cover of sediments. A two dimensional model study indicates that the basement materials may belong to the body causing the anomaly with the exception of the basement material that forms the landward peak of the ridge, which is non-magnetic.     

Ocean Drilling Program (ODP) deep sea coring techniques

The coring techniques and systems of the Ocean Drilling Program (ODP) were developed to satisfy a scientific need for better quality and improved recovery of oceanic core samples. Some of the ODP systems in use today evolved from refinements to earlier systems developed by the Deep Sea Drilling Project (DSDP) or were adaptions of available industry technology. Other systems were conceived and designed by DDP engineers. The evolution of these progressive scientific coring systems began with the Rotary Core Barrel (RCB) used by the DSDP and proceeded to the highly advanced Diamond Coring System (DCS) currently under development by ODP engineers. During the evolution several other key systems were developed. These included hydraulic piston coring, extended coring, pressure coring, bare rock spudding and several systems using high speed diamond coring technology. These include the positive displacement coring motor (PDCM), the Navi-drill core barrel, and a top driven diamond coring system (DCS). This article describes the evolution and conceptual design of these systems including the required bottom hole assemblies, and the sinker bar/sandline configurations.All data, text, figures, photo's etc. contained within this chapter are public domain and free from copywrite. All rights of ODP, NSF, JOI, or other private individuals to use any or all of this material in future published documents is reserved.     

Numerical modelling of the Sea of Azov's dynamics resulting from narrowing of the mouth of Taganrog Bay

The currents and surface level oscillations in the Sea of Azov ensuing from contraction of the mouth of Taganrog Bay are computed using the grid technique on the basis of a numerical solution to the system of quasi-linear, degenerate, parabolic, partial derivative equations (shallow-water equations). It is demonstrated what changes are to be expected in the dynamics of the Sea of Azov should this project be realized. The computations were carried out for typical wind directions.Translated by Vladimir A. Puchkin.     

Sedimentary sources in the surface and near-surface sediments of the Bay of Bengal

High quartz and low calcium carbonate percentages in the surface sediments of the Bay of Bengal adjacent to the Indian subcontinent result from the massive influx of terrigenous clastics. Fine-fraction (<2 ) mineralogy and heavy minerals in (turbidite) sands suggest that sediments of the western Bengal Fan (high in smectite, sillimanite, garnet) have been derived from peninsular Indian rivers; sediments of the rest of the fan (high in illite, hornblende, epidote) are derived from Himalayan rivers. The sediments on the Ninety East Ridge and in the deep southerly areas beyond the reach of fan deposition result from thein situ alteration of volcanics.     

Respiration rates of copepod larvae and a ciliate from a tropical sea

The respiration rates of copepod larvae and a ciliate (Placus sp.) from a tropical sea were measured with an oxygen electrode method. The general range of body size of these animals was 40–161m (diameter equivalent to a sphere), and the respiration rate measured was 0.00076–0.00176l O₂ (animal)^–1h^–1 (or 2.02–9.05l O₂ (mg wet weight)^–1h^–1) at 25.5–29.2C. There was no marked difference observed between the respiration rates of copepod larvae and the ciliate. The respiration rates obtained here are relatively higher than the rates of other similar sized protozoans found in the literature, but lower than the rate extrapolated from larger planktonic copepods in tropical seas.The present results and other information available on microzooplankton biomass suggest that microzooplankton respiration is of near equal importance to that of net zooplankton in the study of energy flow through tropical, pelagic ecosystems.     

Beggiatoa in microbial mats at hydrocarbon vents in the Gulf of Mexico and Warm Mineral Springs,Florida

Microbial mats were collected from a variety of sites near hydrocarbon vents along the slope in the northern Gulf of Mexico and, for comparison, from Warm Mineral Springs, Florida, USA. A predominant microorganism in each of the mats was the giant bacterium,Beggiatoa. Diameters of the bacterial filaments ranged from about 6 µm to approximately 200 µm. The latter organisms are the largest prokaryotic organisms yet found. All filaments over about 10 µm in diameter contained a large central vacuole, producing a cell with the cytoplasm as a cylindrical tube underlying the cytoplasmic membrane. Sulfur globules were confined to this peripheral layer. Push cores often contained pyrite tubules whose appearance is suggestive of aBeggiatoa origin. Determinations of ¹³C inBeggiatoa mats from vents along the Louisiana slope yielded values in the range of –26.6 to –27.9 (PDB), suggesting an unusually high degree of isotope fractionation (–24.9) relative to the carbon source in the ambient seawater, which is typical of sulfur-oxidizing chemoautotrophs. The presence of S^O (elemental sulfur) within cells ofBeggiatoa resulting from oxidation of H₂S supports the importance of bacterial sulfate reduction processes in the underlying vents for the sustenance of theBeggiatoa mats.     

Very low frequency Ocean Bottom ambient seismic noise and coupling on the shallow continental shelf

Sources of very low frequency (0.01 to 1.0 Hz) ambient seismic noise in the shallow (<100 m) water continental margin sediments are investigated using Ocean Bottom Seismometers (OBS). The predominant seismic motions are found to be due to surface gravity (water) waves and water-sediment interface waves. Actual experimental measurements of seabed acceleration and hydrodynamic pressure are given, including side by side comparisons between buried and plate-mounted OBS units. OBS-sediment resonant effects are found to be negligible at the low frequencies under investigation. Wherever there exists relative motion between the seabed and the water, however, an exposed OBS is subject to added mass forces that cause it to move with the water rather than the sediments. Calculations based on measured seabed motions show that a neutral density, buried seismometer has superior sediment coupling charactersitics to any exposed OBS design.     

Chlorinated hydrocarbons in the North Pacific and Indian Oceans

PCBs, DDT compounds and HCH isomers were detected in the air and surface waters of the North Pacific and Indian Oceans, including the Bering Sea, East China Sea, South China Sea, Bay of Bengal and the Arabian Sea. The general concentrations of each chlorinated hydrocarbon were as follows: water PCBs 0.1 to 1.0, DDT 0.01 to 1.0, HCH 1.0 to 10 ngl ^–1; air DDT 0.01 to 1.0, HCH 0.1 to 10 ng m^–3. PCB concentrations in surface waters were slightly lower than those of the North Atlantic and North Sea previously reported, while DDT concentrations in the air and water were higher. Remarkably high concentrations of DDT and HCH were found in the air off the western coast of India. Also in the Pacific site off Central America, a fairly high concentration of DDT was observed in an air sample. These data suggest that large amounts of DDT and HCH are being used in the tropical zone, especially in southern Asia. Furthermore, high concentrations were observed both in the air and water of the Northwest Pacific between 30°N and 40°N latitude. There is a possibility that both pesticides are not only still being used in lower latitude countries but also in the mid-latitude ones of the Asian continent excluding Japan. In addition to this atmospheric circulation may also contribute to the concentration of these pesticides in the mid-latitudinal zone.     

On nonlinear sea waves and the induced mean flow

The nonlinear modulation of water wave groups is investigated and the interaction equations with induced flows are obtained. The analysis is performed up to the third order of the wave steepness a by treating it as a small parameter in the singular perturbation technique by means of the Krylov-Bogoliubov-Mitropolski method. The equation which governs the development of the wave envelope is found by a modification of the ordinary nonlinear Schroedinger equation for the case of uniform depth. The equations governing the behavior of the induced mean flow are examined by deriving the second order flow when the form of the modulated wave train is prescribed. The present theory can describe the mean flow caused by the radiation stress. Some applications containing the monochromatic wave instability are given to confirm the theoretical results.An outline of this paper was presented at The Ocean Surface Symposium (Sendai, 1984).     

Iodine in the surface water of the ocean

Iodine in sea water of the Pacific was determined with special interest in the relation between iodide and iodate in the surface water of the ocean. The result was discussed with reference to the mechanism of iodide formation proposed byTsunogai andSase. The concentration of iodide varies widely from the lower value than the detection limit to 0.21g at./l, while the concentration of total iodine is nearly constant and the mean value is 0.41g at./l. The vertical profile of iodide often shows the maximum in the surface layer. In the surface layer, the concentration of iodide is higher in warm water (0,10g at./l on the average) than that in cold water of lower temperature than 20° C (0.03g at/l). The highest concentration of iodide among the warm waters is found in the surface water of the equatorial area (0.13g at./l) where the biological productivity is also high. Iodide is generally more enriched in the water having higher biological activity even in the cold water. These results are considered to be compatible with the mechanism of iodide formation proposed.