栖息地分布与种内竞争对刺参集群特征的影响

为探究刺参(Apostichopusjaponicus)种内竞争与栖息地对其群体分布的影响,作者通过缩时摄像机拍摄及视频分析,研究了刺参集群分布特征,结果发现:刺参在礁石栖息地的栖息时间显著高于在缸壁处的栖息时间(P 0.05),刺参对栖息地有明显偏好性;刺参在运动时并未聚集在同一栖息地,而离散分布在多处栖息地中(P 0.05);当密度增大到15头/缸(11.31头/m~2, d=1.3 m)时,刺参在栖息地内的平均聚集率和平均聚集时间比例会显著下降(P 0.05),种内竞争加剧,刺参多呈斑块状分布,在密度过高时种内竞争压力上升,刺参个体间选择分散以减少竞争。本研究查明了实验室条件下栖息地分布和种内竞争对刺参集群特征的影响,可为野外刺参增养殖与资源持续利用提供理论参考。     

Field assessment of biochemical stress indices for the sandworm Neanthes virens (Sars)

Certain biochemical stress responses have been observed in the laboratory for Neathhes virens subjected to various environmental and pollutant stressors. The purpose of the present study was to determine whether similar alterations in these biochemical parameters could be detected in a natural population of N. virens from a contaminated site as compared with animals from a nearby reference site. A site in Portland Harbor, Maine, adjacent to an oil storage facility with oil-contaminated sediments was selected as the experimental study area and the reference site was at Pine Point, approximately 15 miles south of Portland.Animals were collected from the two sites on consecutive days on a bimonthly basis at low tide within a day of the full moon from September 1981 through March 1982. Coelomic fluid samples were taken within 2 h after collection and the animals were then frozen in liquid nitrogen for transport back to the laboratory. Coelomic fluid samples were analyzed for glucose and the frozen tissues were analyzed for glycogen, lipid and free amino acid concentrations.Significant differences in these parameters were often observed between the two populations. The glycogen content of animals from the contaminated site was always less than that of animals from the reference site, whereas the lipid levels were always higher. Significant differences between the free amino acid composition of the animals from the two sites were also observed. Although distinct biochemical differences can be detected between these two populations, the considerable biological variation in these parameters may limit their usefulness for field monitoring investigations for this species.     

Notes on laboratory rearing of juvenile spiny lobsters,jasus edwardsii (hutton) (crustacea: Decapoda: Palinuridae)

Post‐puerulus specimens of Jasus edwardsii (Hutton) have been successfully reared in the laboratory from the puerulus stage for periods of up to 12 months. The spiny lobsters were reared in concrete‐asbestos tanks measuring 2.4m × 37cm × 23cm. The tanks were supplied with a continuous flow of sea water and provided with constant aeration. Empty shells of paua (Haliotis iris Martyn) and rocks from the intertidal zone provided cover within the tanks.

Fresh mussel (Mytilus sp.) was preferred to all other foods tried in feeding experiments. The animals were fed every second day on opened mussels, and occasionally on fresh fish. The juveniles “grazed” actively on the calcareous algae (Corallina officinalis L.) present on the rocks.

The animals were extremely sensitive to pollution. To reduce pollution risks all sediments were removed from each tank, as the interstices between them were found to harbour uneaten food particles. Any uneaten foods were siphoned out every second day and each tank was thoroughly cleaned every six to eight weeks.

From a total of over 3,300 animals collected since November 1965, over 800 are presently being reared in the laboratory and various aspects of their ecology studied. The first step in raising larger adult sizes from juvenile stages in the laboratory is clearly possible.     

Comparative analysis of the diets of smaller odontocete cetaceans along the coast of southern Africa

The diets of 20 species of smaller Odontoceti were investigated. The material was collected along the southern African coast between 1966 and 1990 and included 158 strandings, 29 animals killed incidentally and 49 animals caught under permit. In total, 57 species in 34 families of fish and 89 species in 24 families of cephalopods were identified from remains in the 236 stomachs investigated. Most Odontoceti fed on commercially unexploited cephalopods and fish, or on fish below commercially utilized sizes. However, the average sizes of anchovy and pilchard eaten by Delphinus delphis and Lagenorhynchus obscurus were larger than the sizes most frequently taken commercially. The species composition of the prey items reflected the onshore-offshore distribution (and possibly the foraging depths) of different Odontoceti. Tursiops truncatus and Cephalorhynchus heavisidii were closest to shore and D. delphis and L. obscurus over the continental shelf. The rest were distributed around the shelf-edge and just off the edge. A comparison of the stomach contents of stranded and non-stranded D. delphis, L. obscurus and C. heavisidii demonstrated that results from stranded animals are biased.     

Swimming behaviour in a pycnogonid (note)

Four adult specimens of the pycnogonid Ammothea magniceps were observed swimming at the sea surface at night at Kaikoura. Even after capture the animals continued to swim at the surface of the water for over an hour. Such swimming behaviour is considered important in explaining the presence of “benthic” animals in plankton samples.     

5种水产动物血红细胞形态显微观察比较及进化分析

采用Hemacolor染料对泥蚶（Tegillarca granosa）、毛蚶（Scapharca subcrenata）、鲫鱼（Carassiusauranus）、牛蛙（Rana catesbeiana）、中华鳖（Pelodiscus sinensis）等5种水产动物血细胞涂片进行染色,利用光镜和扫描电镜两种方法对5...     

浙江南部沿岸附着生物与钻孔生物——Ⅲ.南几岛的附着生物与钻孔生物生态

附着生物与钻孔生物的分布是随着外界环境因素的不同而异,一般都受到了温、盐、流等外界因素的影响.因此,合理布局试验点,是全面了解我国沿岸附着生物生态,系统地掌握生物学资料的关键.十几年来,为了满足国防和国民经济建设上的需要,我所及其他单位已对中国沿岸的附着生物和钻孔生物做了大量的试验和调查研究,仅东海沿岸,从长江口、舟山海区、石浦到福建沿岸就做了近20处的试验.     

Feeding,defaecation and absorption efficiency in the Cape rock lobster jasus lalandii

In order to estimate the absorption rate of carbon by the palinurid lobster Jasus lalandii, the net quantity of ingested carbon and gross carbon content of faeces must be known. Experimental animals were selected for consistent feeding and subsequently starved for 72 h. Ingested carbon was established by determining the carbon content of the prey mussel Choromytilus meridionalis under controlled aquarium conditions through five eating/defaecating cycles. The losses of particulate and dissolved organic carbon due to messy feeding were measured and amounted to 20,1 per cent of the food presented. Of the net carbon ingested, 20,5 per cent was voided as faeces, thereby giving a mean absorption efficiency of 79,5 per cent (n = 15, SD = 4,6%). J. lalandii, as a top carnivore in the inshore Benguela ecosystem, is therefore highly efficient in the utilization of ingested carbon. In animals of commercial size (≥ 89 mm carapace length), small variations in absorption efficiencies exhibited were neither dependent on body mass nor ingested mass, but may rather be attributed to scatter inherent in all biological systems.     

Effect of reproductive state and sex on movement and food consumption of western rock lobster (Panulirus cygnus) in a tank environment

The catchability of western rock lobsters (Panulirus cygnus) during the spawning season (October and November) may be affected by their reproductive state. In this study, movement and food consumption of males and females of different reproductive states held in laboratory tanks were measured as proxies for catchability. Density of lobsters was found to influence both movement and food consumption. Solitary animals and, to a lesser extent, two lobsters per tank, were more sedentary and consumed significantly less food than animals at a density of three or four specimens per tank. Tests using three animals per tank confirmed that mature, unmated, and ovigerous females carrying early stage eggs moved more frequently away from their shelters than males or females carrying late stage eggs (P < 0.0001, d.f. = 3). Further, unmated females and females with early stage eggs recorded significantly higher food consumption (P < 0.0061, d.f. = 3) compared with males or females carrying late stage eggs. The greater time spent away from shelter and also food consumption make unmated females and females with early stage eggs likely to be more catchable than males or females with late stage eggs. As catchability is commonly used to obtain population estimates from survey data, these results have implications for surveys which use catch rates of breeding animals as indicators of egg production.     

Atlantic humpback dolphins Sousa teuszii in the Saloum Delta (Senegal): distribution,relative abundance and photo-identification

During October and November 2015, the first systematic survey of Sousa teuszii was carried out in the Saloum Delta (Senegal, West Africa), comprising 1 617.5 km of boat-based survey coverage. Thirty sightings were recorded in the Saloum and Diomboss rivers, and along the southern coastline. Dolphins were also observed entering the Bandiala and Djinack channels, and travelling across the border into Gambia. The initial sighting locations were 0.043–1.192 km from shore, and tracked dolphins did not move more than 2.082 km from shore. Groups comprised 1–29 animals (mean 9.3 animals), and at least three neonate calves were observed during November. The overall relative abundance was 0.018 sight. km^?1 and 0.175 ind. km^?1. Sightings were concentrated in the Diomboss where relative abundance reached 0.037 sight. km^?1 and 0.331 ind. km^?1. Non-intensive photo-identification produced a minimum population size of 103 animals, the highest recorded for S. teuszii anywhere in its range. Photo-identification also confirmed a movement of individuals between different parts of the Saloum Delta. Combined travel–forage dominated the behaviour. Dolphins were photographed capturing mullet (Mugil sp.) on three occasions. The distribution, population size and movements of S. teuszii are discussed in relation to management.     

Assessment of imposex in the common whelk, Buccinum undatum (L.) from the Eastern Scheldt, The Netherlands

Several cruises were carried out on the Eastern Scheldt between September 1992 and March 1995 to study the frequency and appearance of imposex in the common whelk, Buccinum undatum L. Incidences of imposex were always > 90%. For the first time several stages of imposex in Buccinum undatum are reported. The evolution of imposex in the whelk seems to follow a similar route as in Hinia reticulata belonging to the same superfamily (Buccinacea). Tissue organotin concentrations of animals collected in February 1995 showed the highest organotin concentrations in the digestive gland and lowest in the foot of the animals. Concentrations of phenyltin compounds (up to 625 ngSn/g DW) were much higher than those of butyltin compounds (up to 40 ngSn/g DW).     

Uptake and transformation of C-labelled tributyltin chloride by the dog-whelk, nucella lapillus: Importance of absorption from the diet

The importance of the diet as a source of tributyltin (TBT) in Nucella lapillus was studied using [¹⁴C]tributyltin chloride. In N. lapillus provided with prelabelled mussels, Mytilus edulis, in labelled water (mean 20·5 ng/litre TBT) the rate of accumulation of total ¹⁴C was 2–3 times that in unfed animals. Owing to its degradation in the tissues of both fed and unfed animals, concentrations of [¹⁴C]TBT tended to reach a plateau after only 28 days. However, total concentrations of ¹⁴C were still increasing after 49 days. Under experimental conditions (15°C, ample food, no disturbance) the diet accounted for about 50% of the body burden of TBT in N. lapillus after 49 days exposure: concentration factors (dry tissue/water) for [¹⁴C]TBT in both male and female N. lapillus were similar at about 60 000 in fed and 30 000 in unfed animals. It is concluded that the diet may contribute less than half of the body burden of TBT found in natural populations subjected to life-long exposure.     

Adaptations for scavenging by three diverse bathyla species, Eptatretus stouti, Neptunea amianta and Orchomene obtusus

Many deep-sea animals derive part of their nutrition from rare and unpredictable food falls. However, traits that allow organisms inhabiting the sea floor to exploit carrion are poorly understood. We found in laboratory experiments that hagfish (Eptatretus stouti), gastropods (Neptunea amianta) and amphipods (Orchomene obtusus) survived extended periods of starvation, in some cases for more than a year. When exposed to odors emitted from carrion, most individuals of E. stouti and O. obtusus began searching for food within seconds, whereas none responded to the scent of the live prey. In contrast, the slow crawling N. amianta readily consumed carrion but showed no apparent response to any odor solutions tested. Because more motile animals exhibited lower thresholds for response to signal molecules, sensitivity to chemical cues appears related to species mobility. Hagfish were also found to defend carrion from some competitors by releasing slime when feeding. Though varying dramatically in size, morphology, locomotive ability, and phylogeny, these three species all possess traits well suited for a scavenging lifestyle.     

Using platforms of opportunity to determine the occurrence and group characteristics of orca (Orcinus orca) in the Hauraki Gulf,New Zealand

We present the first fine-scale data relating to the occurrence and group characteristics for killer whales (Orcinus orca) in the Hauraki Gulf, New Zealand, as observed from a platform of opportunity (PoP). Group size and composition were examined in relation to water depth and sea surface temperature (SST). From 2000 to 2010, 119 orca encounters were recorded, involving 1 to 18 animals. The encounter rate varied seasonally, being highest in austral spring and lowest in summer. Water depth in which whales were observed was significantly affected by group composition. Sixty-seven percent of groups contained immature animals. Group size was highly skewed towards smaller groups comprising two animals. While this study illustrates that PoPs can be used to indicate the occurrence and group characteristics of highly mobile social species, biases clearly exist. Through identifying such inaccuracies, we present recommendations on how future data should be collected to minimise error and improve datasets for scientific use.     

Removal of intertidal algae by herbivores in experimental frames and on shores near Auckland

When grazing animals are excluded from any intertidal area algae grow unchecked. In screened frames on the shore where predation experiments are in progress such growth is undesirable. A number of shore herbivores were tested for their ability to remove such algal growth and prevent its reformation without interfering with the main experiments. Only the browsing species Lunella smaragda and Melagraphia aethiops were capable of removing large algal growths, and these species were acceptable food for some of the predatory species. The complex inter‐relationship between grazing species and shore zonation at several places near Auckland is discussed.     

Seasonal variations in the activity of bioindicators in the Garm research area

It was established that the electric activity of slightly electric fish Gnathonemus leopoldianus and the motion activity of somiks Hoplosternum thoracatum and cockroaches Blaberus craniifer, which were kept in a thermally stabilized room, regularly change throughout the year. Changes in the activities of different species of animals are coordinated. In all of the cases, the activity maximum falls on the spring-summer period. Possible factors responsible for seasonal variations in the electric and motion activities are discussed, and their forms are compared with the character of seasonal variations in different geophysical processes.     

基于核酸适配体的差减荧光法检测鳗弧菌(Vibrio anguillarum)

鳗弧菌(Vibrio anguillarum)可感染鳗鲡、虹鳟和大菱鲆等多种水产动物,是水产养殖中的一种重要病原菌,对其进行快速检测是病害防控的前提和基础。利用鳗弧菌与其核酸适配体之间有较强的亲和特异性,首次建立了一种基于核酸适配体的可定量检测鳗弧菌的差减荧光法。该方法对鳗弧菌有较好的特异性,对鳗弧菌的检测荧光值是其他菌(溶藻弧菌、哈维氏弧菌、铜绿假单胞菌、变形假单胞菌、嗜水气单胞菌、迟钝爱德华氏菌和大肠杆菌)的4～11倍,对鳗弧菌的最低检测限为10²CFU/mL,可用于10²～10⁸CFU/mL的范围内的定量检测。通过对不同盐度海水和鱼体组织样品进行加标回收检测,结果表明,回收率和相对标准偏差等指标均符合相应的标准,说明该检测方法可用于海水样品和水产动物组织中鳗弧菌的检测。     

Metabolism of tributyltin oxide by crabs, oysters and fish

Bis(tributyltin) oxide (TBTO) is widely used as an antifouling agent in various antifouling paints. Thus, some marinas have TBTO concentrations as high as 2 μg/liter.¹ These concentrations can be toxic to zooplankton.² The objectives of the present study were to determine the ability of a number of marine animals, including crabs, oysters and fish, to metabolize TBTO. Earlier work showed that extracts of rat liver were able to metabolize TBTO to a variety of metabolites, e.g. β-hydroxybutyldibutylin.³ The role of the cytochrome P-450 dependent mixed-function oxygenase system in oxidizing TBTO in marine animals was also of interest. Both in vivo (uptake of ¹⁴C-TBTO from food or water) and in vitro studies demonstrated that all the animals were able to metabolize TBTO. The oysters metabolized TBTO at a much slower rate than the other animals. The mixed-function oxygenase system from hepatic tissues of the various animals was able to metabolize TBTO by forming a number of hydroxylated metabolites.     

Reflection on the first five years of South Africa’s Acoustic Tracking Array Platform (ATAP): status,challenges and opportunities

The Acoustic Tracking Array Platform (ATAP) is a marine science programme that monitors the movements and migrations of inshore marine animals along the South African coastline. Acoustically tagged animals are monitored by an expanded network of approximately 100 automated data-logging acoustic receivers moored at strategic node sites, from Cape Point in the west to the South Africa–Mozambique border in the east. During five years since its inception, in 2011, the ATAP has achieved outstanding progress in terms of the numbers of animals and the variety of species tagged. To date, the ATAP has yielded over 2.6 million detections from more than 700 acoustically tagged animals, representing 27 different species from 20 families, including the African penguin Spheniscus demersus, fishery-at-risk species (e.g. dusky kob Argyrosomus japonicus) and iconic elasmobranchs (e.g. white shark Carcharodon carcharias). Following a period of considerable equipment loss in 2014, refinement of the receiver network was required and the deeper receivers at each site were decommissioned without influencing the integrity of the nationwide array. The platform, managed by the South African Institute for Aquatic Biodiversity, ultimately represents a low-cost method of collecting long-term data that currently benefits approximately 25 researchers from 14 organisations. Case studies are presented to expose the opportunities provided by the ATAP, which will undoubtedly yield new discoveries and provide a greater understanding of the movement patterns and migrations of a wide variety of inshore marine and estuary-associated species.     

Biology and life cycle of Natatolana borealis Lilj.1851, a scavenging isopod from the continental slope of the Mediterranean

The biology of Natatolana borealis, a deep-sea (−500 m) scavenging isopod that lives on the continental slope of Mediterranean canyons, was studied in the field and in the laboratory. Animals were collected at two stations using a baited trap. Whatever the site at which the animals were collected, a strong correlation was always found to exist between their length and weight, which shows variably marked seasonal variations. The growth pattern was modelled by fitting the Von Bertalanffy growth equation to the length of animals of various ages. The lifespan was estimated to be 6 years in the case of the largest animals collected. Sexual maturity is presumably reached at the age of 2.5 years. Not all the females were found to be sexually active at the same period of the year. A scheme of reproductive cycle is proposed for the mature females. It seems likely that each female may produce at least four broods during her lifetime. The emergence of offspring occurs at various times throughout the year, resulting in successive cohorts. At some periods in the year, some developmental stages were lacking in the samples, possibly owing to alimentary behaviour that helps to protect the animals from predators.