Comparative studies of inorganic carbon utilization inEmiliania huxleyi and some non-calcifying marine microalgae

Inorganic carbon utilization in the non-calcifying marine microalgae,Nannochloropsis oculata, Phaeodactylum tricornutum andPorphyridium purpureum was compared with high- and low-calcifying strains ofEmiliania huxleyi grown in artificial seawater medium aerated with either air (0.03% V/V CO₂) or CO₂-free air. For high-calcifying strain ofE. oculata andP. tricornutem, similar growth patterns were observed in air-and CO₂-free air-grown cultures.P. purpureum showed a less final cell density in CO₂-free air than in air-grown culture. However, low-calcifying strain ofE. huxleyi was able to grow only in air-grown culture, but not in CO₂-free air-grown culture. Measurements of alkalinity, pH, concentration of dissolved inorganic carbon (DIC) and free CO₂ showed different patterns of DIC utilization. WithN. oculata, P. tricornutum andP. purpureum the pattern of DIC utilization was characterized by an increase of pH and a decrease of DIC but a constant alkalinity in the cultures aerated with air or CO₂-free air, suggesting that bicarbonate utilization was concomitant with an efflux of OH⁻. Both alkalinity and pH were maintained rather constant in air-grown culture of low-calcifying strain ofE. huxleyi, suggesting that diffusive entry of CO₂ could meet the requirement of DIC for its photosynthesis and growth. High-calcifying strain ofE. huxleyi, however, showed a pattern of decrease of alkalinity and DIC but an almost constant pH, indicating that bicarbonate was the major form of inorganic carbon utilised by this organism and bicarbonate uptake is unlikely to be accompanied by an efflux of OH⁻. The final pH values reached byN. oculata, P. tricornutum andP. purpureum in a closed system were 10.75, 10.60 and 9.85 respectively, showing that bicarbonate utilisation is concomitant with an efflux of OH⁻. While the final pH of 8.4 in high-calcifyingE. huxleyi suggests that bicarbonate utilization was not accompanied by an efflux of OH⁻. Contribution No. 3557 from the Institue of Oceanology, Chinese Academy of Sciences. This work was supported by NERC grant GRE3/7853 U. K. and partly supported by Bio-Engineering Center, SSTC 96-C01-05-01.     

广西海洋资源概况及开发刍议

广西海洋资源丰富 ,品种繁多 ,为了更好开发 ,建议从法制、宣传教育、普及海洋资源有关知识 ,提高人们整体素质做起 ,进一步抓住机遇 ,合理开发 ,才能取得良好效益 ,达到预定目标     

辽宁省海水入侵成因及防治措施

海水入侵是在自然和人为因素综合作用下形成的地质灾害。辽宁省海水入侵自 70年代以来呈逐渐增加趋势 ,具有入侵面积广、发展速度快等特点。目前海水入侵已成为我国较为突出的地质灾害 ,给工农业生产和人民生活造成很大危害。针对海水入侵形成机制及危害程度的地域性 ,目前已采取不同的防治措施 ,如控制开采 ,人工回灌 ,修建地下水库等 ,取得一定效果。     

MARINE HAZARDS IN COASTAL AREAS AND HUMAN ACTION AGAINST THEM-A CASE STUDY OF THE CHANGJIANG RIVER ESTUARIES AND QIANTANG RIVER

INTRODUCTIONMarinehedsresultinginenomouslossestousuallyPOpulousandeconondcallyde-veloPedlowandcoastalandestUdrineregi0nsareforngseriousattenti0n.msarticlediscussesP0ssiblepreventivemeasaresagainstInariflehazaIdsbasedonacasestUdyoftheestUedesoftheChanaiiangffiverandQiantanffiver-ThesetwoareasoftheChanaiiangDeltaareboicalofc0astalregionsfreqUenilysufferingfromdrinehazanls,andarethemostsociallyandeconondcallydeveloPedregionsinChina.Theirinhabitantshaveahist0-ryofmorethanathouSandyeare0…     

COMPARATIVE STUDIES ON STRUCTURAL FEATURE OF AGAR POLYSACCHARIDES FROM PORPHYRA HAITANENSIS GROWN IN SOUTH AND NORTH CHINA

The structural feature of agar polysaccharides from Porphyra haitanensis grown in south China and transplanted to the north was investigated by fractioriation on DEAE - Sephadex A 50, chemical analysis, and infrared and 13C - NMR spectroscopy.The agars composed mainly of Charged molecules were eluted from DEAE - Sephadex A 50 with 1.0 mol/L- NaCl solution from the southern P. haitanensis and with 0.5 mol/L NaCl from the northern one. The 13C-NMR spectra showed that agarobiose [(1 -3)- B D - galactopyranosyl - (1 -4)-3,6-anhydro-a-L-galactopyranose] and the biological precursor of agarobiose [(1-3} B-D-galactopyra nosyl- (1-4)- 6- sulfate-a- L-galactopyranose ] were the major disaccharide repeating units in the charged fractions. The content of the biological precur sor in the agar polysaccharides from southern P. haitanensis was higher than that in the northern one, the content of the biological precursor extracted from cold water was higher than that from hot water, and the content of 6- OMe-D-galactos