APPLICATION OF CLAYS TO REMOVAL OF RED TIDE ORGANISMS Ⅱ.COAGULATION OF DIFFERENT SPECIES OF RED TIDE ORGANISMS WITH MONTMORILLONITE AND EFFECT OF CLAY PRETREATMENT

Study on the coagulation of four species of red tide organisms(Nitzschia pungens,Skeletonema costatum,Prorocentrum minimum and Noctiluca scintillans)with montmorillonite,and the effect ofmontmorillonite pretreatment on the coagulation shows that the capability for montmorillonite to coagu-late with them is in the order:N.pungens>S.costatum>P.minimum>N.scintillans.Thecoagulation is discussed from the aspects of the structure,shape,size,movement,habit,etc.of differ-ent species and the results are explained theoretically.The experimental results also indicate that the treat-ment of montmorillonite with acid can enhance its coagulating capability.This is due to the fact that Al(OH_2)_6~(+3),exchanged from the clay lattice by hydrogen ion H~+,forms hydroxy-aluminum polymers on thesurface of the montmorillonite.The hydroxy-aluminum polymers positively charge and increase the positivecharacteristic of the clay surface,and also serves as a bridge between adjacent surfaces of particles.Thesetwo functions enhance     

Applications of immuno-magnetic bead and immunofluorescent flow cytometric techniques for the quantitative detection of HAB microalgae

Overthelastseveraldecades,harmfulalgalblooms(HABs)havebecomeaseriousenvironmental problem in many parts of the world. A rapid and accurate detection process for HAB algae has yet to be developed. Heterosigma akashiwo is one of the most important HABs species in China. The objective of this study was to develop an immunologic technique that can rapidly and sensitively count H. akashiwo cells. Five HABs species (Alexandrium catenella, Thalassiosira sp., Cryptomonas sp., Alexandrium tamarense and Symbiodinium sp.,) were used in this study to evaluate the analysis process we developed. A polyclonal antibody with high titers against H. akashiwo was obtained by injecting H. akashiwo cells into rabbits. Immuno-magnetic beads (IMB) were produced via conjugated polyclonal antibodies with magnetic beads and applied to isolate and count H. akashiwo cells from the culture. Results show that 66.7%-91.6% of the cells were captured from unialgal culture by IMBs, and only 5.3%-12.5% of the four other HAB microalgae species were captured, indicating that the constructed IMBs combined specifically with the H. akashiwo cells. At the same time, flow cytometry (FCM) sorting was exploited to screen H. akashiwo cells after labeling with FITC conjugated polyclonal antibodies. Using the FCM technique, 91.7% of the targeted cells were sorted out from mixed microalgae samples in just a few minutes. These results indicate that both antibody-involved IMB and antibody-based FCM techniques are highly effective at detecting and quantifying HAB species. These techniques, especially immuno-magnetic separation, have low associated cost, and are fast and simple processes compared with other techniques currently in use.     

城市生长与形态双维分形模型改进及应用

分形方法是测度城市生长与形态演化的重要方法,然而其标度区范围的自然识别依然存在瓶颈。本文采用二阶导数识别半径法标度区界线,结合经典计盒法模型提出改进的双维分形标度律测算方法,以郑州市为案例区验证模型的精度与效度,综合解析郑州城市空间形态结构的生长演变特征。结果显示:① 利用二阶导数自动识别半径法标度区界线可显著提升分形模型拟合效果,拟合精度R²由0.920提升至0.996以上;② 郑州城市空间结构存有双标度现象,城市生长扩张并非标准环形波状推进,半径法模型仅在“城市中心边缘—建成区内侧”的环状范围拟合有效,城市结构可能存有自仿射生长或随机多分形特征;③ 1982—2020年郑州城市形态演化为明显的中心蔓延模式,职能用地的空间配置整体均匀程度偏高,功能分异程度较低,存有城市景观较紊乱,系统效率不高的隐患。本研究对半径法分形模型中拟合精度难以提升的应用瓶颈作出了突破性尝试,为城市生长模拟与城市空间结构演变分析提供了一种新方法,丰富了城市分形研究。     

Study on the isotherms of the interaction between suspended particles and Cu (II) in the Huanghe River

The isotherms of the interaction between the suspended particles and Cu²⁺, and the effects of lysine and asparaginic acid on the isotherms in the Huanghe (Yellow) River were studied by applying the theory and method of interfacial stepwise ion/coordination particle exchange. We obtained a new stepped river isotherm, formed by two curves joined together with a “plateau” in the middle. The adsorption equilibrium constantsK ₁ andK ₂ were calculated by using the isothermal equation of surface stepwise ion exchange. Amino acid in small amount promotes exchange adsorption of the suspended particles with Cu²⁺. The degree of promotive action relates to the isoelectric point of amino acid. The promotive effect of lysine is bigger than that of asparaginic acid. Project 29361001 supported by NSFC.     

Property and performance amelioration of pelagic clay from the East Pacific

To make use of the pelagic clay as polymer filling, the properties of clay sediments from the ocean investigation zone of China in the East Pacific were studied by the SSA, XRF, ICP/MAS, FTIR, XRD, SEM, DTA/TG and granularity distributing etc. , and experiments were settled to improve the whiteness and activation of the clay based on these data. Compared with land clay, pelagic clay holds many particular features, such as fine particles and incompact accumulation, worse crystallization and more defects, high activity, complex mineral and chemical components, and low whiteness etc. Processing the purified pelagic clay with acids and zinc, then baked it at different temperatures, the whiteness of clay can be increased from 23.8% to 73.1%, and the optimized conditions is： consistency of vitriol 25%, ratio of clay to water 4： 1, reaction time 4h, reaction temperature 90℃, dosage of zinc 2.0 g/L, and baking temperature 700℃. And the SSA of whited clay is increased too. Using the dry milling method to modify the pelagic clay with organic reagents, the optimized technique is KH550, concentration 1.5%, reaction time 20 min. XRD, FTIR and SEM testing indicate that the mechanism of organic activation was mainly surface absorbing and chemical combination secondly.     

Properties and organic activation of ocean clay in the East Pacific

In order to make use of ocean clay reasonably and excavate its potential applications the properties of ocean clay in Chinese investigating area of the East Pacific have been tested with the chemical analysis, XRFS, ICP/MAS, granulometry and SSA, etc. The results show that the clay sediments of this area are rich in clay minerals, fine granularity, bigger SSA, bigger water absorbability and lower ion changing ability etc. To improve the adaptability of ocean clay and macromolecule materials, using the dry milling method with silane, aluminate, stearic acid coupling agents etc the properties of ocean clay are improved. The effect has been evaluated by testing the water absorbing of the modified ocean clay and their dispersing in paraffin liquid. The result is that KH550 is better than any other active reagents, and the best activation technics is that 1.5% (volume) of KH550 quantity, 20min of milling time. The DTA, XRD, SEM and IR analyses and SSA testing show that the organic activation can not change the mineral structure but can densify the structure of clay, and activation mechanism is chemical covered mainly and chemical combined secondly.     

Properties and organic activation of ocean clay in the East Pacific

In order to make use of ocean clay reasonably and excavate its potential applications the properties of ocean clay in Chinese investigating area of the East Pacific have been tested with the chemical analysis, XRFS, ICP/MAS, granulometry and SSA, etc. The results show that the clay sediments of this area are rich in clay minerals, fine granularity, bigger SSA, bigger water absorbability and lower ion changing ability etc. To improve the adaptability of ocean clay and macromolecule materials, using the dry milling method with silane, aluminate, stearic acid coupling agents etc the properties of ocean clay are improved. The effect has been evaluated by testing the water absorbing of the modified ocean clay and their dispersing in paraffin liquid. The result is that KH550 is better than any other active reagents, and the best activation technics is that 1.5 % （volume） of KH550 quantity, 20min of milling time. The DTA, XRD, SEM and IR analyses and SSA testing show that the organic activation can not change the mineral structure but can densify the structure of clay, and activation mechanism is chemical covered mainly and chemical combined secondly.     

Property and performance amelioration of pelagic clay from the East Pacific

To make use of the pelagic clay as polymer filling, the properties of clay sediments from the ocean investigation zone of China in the East Pacific were studied by the SSA, XRF, ICP/MAS, FHR, XRD, SEM, DTA/TG and granularity distributing etc. , and experiments were settled to improve the whiteness and activation of the clay based on these data. Compared with land clay, pelagic clay holds many particular features, such as fine particles and incompact accumulation, worse crystallization and more defects, high activity, complex mineral and chemical components, and low whiteness etc. Processing the purified pelagic clay with acids and zinc, then baked it at different temperatures, the whiteness of clay can be increased from 23.8% to 73.1%, and the optimized conditions is : consistency of vitriol 25%, ratio of clay to water 4:1, reaction time 4h, reaction temperature 90℃, dosage of zinc 2.0 g/L, and baking temperature 700℃. And the SSA of whited clay is increased too. Using the dry milling method to modify the pelagic clay with organic reagents, the optimized technique is KH550, concentration 1.5%, reaction time 20 min. XRD,FTIR and SEM testing indicate that the mechanism of organic activation was mainly surface absorbing and chemical combination secondly.     

Chemical mechanism of flocculation and deposition of clay colloids in coastal aquifers

Seawater intrusion has become one of serious environmental problems in coastal areas. During the replacement of saline water by fresh water in the aquifers, in-situ clay could be released, transport and deposit in the porous media due to the change of hydrodynamic and geochemical conditions, which resulted in the increasing of particle size, plugging of pores and reduction of the permeability. Batch experiments and sand column experiments were explored to study the relationships between the flocculation of in-situ clay and geochemical conditions, by changing ionic strength and ionic type of clay suspension. Column outflow was analyzed for suspended particles and electrical conductivity. The total percentage of colloid straining and interception distribution in porous media was calculated. The results indicate that porous media had an effect on the interception of clay colloid particles with about 10 percent clay colloids captured due to the rough surfaces and spatial structure of porous media. Ionic strength played a key role on the permeability reductions. The higher ionic strength is, the greater the amount of colloidal particles trapped. Ionic type also had a significant effect on the interception of clay colloid particles. Ripening was the main mechanism for the interception within porous media when the bulk solution was potassium chloride while blocking happened when the bulk solution was sodium chloride. The distribution of clay colloids in porous media was heterogeneous. The closer to the sand column inlet was the less interception of clay colloids was. The results can provide the scientific basis for preventing the water sensitivity during the process of salty aquifer restoration.     

ELECTROCHEMICAL CORROSION BEHAVIOR OF STEEL IN THE JUNCTURE AREA OF SEA CLAY AND SAND

Corrosion behavior of steel in the juncture area of sea clay and sand was studied. The results show that galvanic corrosion occurred between steel samples buried in sea clay and sand, and that the polarity of the galvanic cell reversed after some testing later. The cause of reversing of polarity is discussed.     

山东寺庄金品位参数统计和分形特征及其对矿化信息

采集寺庄金矿区82个钻孔的金品位数据4597个,按照100m的标度分析其在垂向上的均值、标准差、变异系数和大于工业品位的矿石所占比例等统计参数,对比其在垂向上的分形分维值特征,发现如下规律：寺庄金矿区深部矿体明显优于浅部矿体,以-700- -800m区间为矿化中心,垂直分布至少500m以上;通过围岩蚀变分带特征、矿石组合特征、井下脉体相互穿插关系和金品位分形分维值研究,说明该区金成矿时间上至少经历了3次地质成矿作用的叠加,其中显著成矿作用发生在后2次,在空间上深部（-1000m以下）具有较大的找矿潜力;金     

A model describing the time course and variability in toxicity of hydrophobic chemicals to aquatic organisms

The growth of Chlorella marine, Nannochloris oculate, Pyramimonaos sp., Platymonas subcordiformis and Phaeodactylum tricornutum exposed to chlorobenzene, 1,2-dichlorobenzene, 1,2,3,4-tetrachlorobenzene and pentachlorobenzene was tested. The Boltzman equation was used to describe organism growth. The time course for uptake of hydrophobic organic chemicals (HOCs) by aquatic organisms was expressed by incorporating growth and, if desired, the effect of metabolism into the HOC bioconcentration process. The probability of any given concentration of HOCs in the organisms causing a specified toxic endpoint was expressed with a modified Weibull distribution function. The combined bioconcentration and probability equations were tested with data for time course of incubation of algae exposed to chlorinated benzenes (CBs). A set of parameters, including the uptake rate constant k ₁, the elimination rate constant k ₂ and thereafter the bioconcentration factor on a dry weight basis, BCF _D, the critical HOC concentration in the organism resulting in a specified toxic endpoint, C _A ^* , and the spread factor, S, could be obtained by fitting only experimental data for percent growth inhibition(%)-time-CB exposure concentration. The average coefficients of variation within CBs were 15.2% for BCF _D, 21.0% for k ₁, 18.3% for k ₂, 8.1% for C _A ^* and 9.7% for S. The variability in toxicity (such as EC₁₀, EC₅₀, EC₉₀) derived from the model equations agreed well with those experimentally observed.     

塌陷区地形分形生成方法与应用

由于煤炭开采而形成的塌陷区地形形态复杂,本文利用分形插值理论模拟了塌陷区地形。在Matlab语言环境下,实现了塌陷区基于遗传算法求取无标度区最优解和基于FBM分形插值算法,在此基础上求解分形特征参数H和σ。对概率积分法预计的数据进行分形内插绘制地表下沉图,取得了理想的效果。     

Practical method and application study for predicting cyclic accumulative deformations of the saturated soft clay

The distribution of saturated soft clay is greatly wide in China. The current main measures adopted to deal with soft soil foundations may lead to environmental pollution, even some engineering accidents may happen on soft soil foundations. In order to solve engineering problems of saturated soft soil foundations well, researches of mechanical properties of them are necessary. One of the most important mechanical characteristics of saturated soft clay is its cyclic accumulative deformation under cyclic loadings. For saturated soft clay, the cyclic accumulative deformation is similar to the creep behavior under static loadings. Therefore, the cyclic accumulative deformation is equivalent to the creep, the number of loading cycles is seen as the time, and this study develops a practical method for predicting the cyclic accumulative deformation of saturated soft clay with the creep theory. The method is a pseudostatic elasto-plastic finite element method implemented by ABAQUS software. A fitted equation between cyclic accumulative strain and number of loading cycles and the empirical relationship of parameters of fitted equation were established with a series of cyclic triaxial compression tests. Then with this empirical relationship of parameters, the method developed by this study was employed to predict the cyclic accumulative deformation under cyclic triaxial tension tests. Predicted results were in good agreement with test results, and the effectiveness of this method was thus validated for different stress states. The method was then applied in analyzing the cyclic accumulative deformation for soft soil foundation of a pile-supported wharf structure.     

地学现象的分维与标度关系——以中国大陆山系为例

分形理论产生以后,已经在许多领域里取得了较为广泛的应用,但是在分形维数的计算方面仍存在一定的问题,最根本的问题之一是大量相关研究往往忽略了标度问题的影响,而将计算出的分维结果随意比较。本文则是以中国大陆山系为例,对地学现象的分维与标度的关系进行了研究,得出:(1)中国大陆山系的分形性质是客观存在的,其分形性质是不以测算标度而出现变化的,是始终存在的;(2)虽然在不同的测算标度下,中国大陆山系的分形性质始终是客观存在的,但是随着测算标度范围的变化,中国大陆山系的分维也会相应地出现一定的变化,即中国大陆山系客观存在的分形性质是绝对的,而分维则是一个相对的值,是随着测算标度而出现变化的;(3)分维不仅随着测算标度的变化而出现一定的变化,而且,分维是测算平均标度的函数,即D=f(r)(式中:r为测算平均标度,D为分维)关系成立;(4)因为分维值会随着不同标度范围的变化而出现相应的变化,所以,如果要进行分维之间的比较,则应选择相同的标度范围和标度值点。     

Effects of environmental factors on classification of loessderived soils and clay minerals variations,northern Iran

Land-use type under different topographic conditions and human activities affects soil development. We investigated the effects of land-use, topography and human activity on soil classification changes in the Toshan watershed in northern Iran. Seven representative pedons derived from loess parent materials were studied on different land-uses and topographic positions. The studied pedons in forest (FO) on backslopes and footslope were classified as Calcic Haploxeralfs and Typic Haploxeralfs, respectively. The soils in abandoned lands (AB) and orchards (OR), where formerly under natural forests, located on the shoulder and backslopes positions were classified as Calcic Haploxeralfs and Vertic Haploxeralfs, respectively. Well-developed argillic horizons as indicators for higher degrees of soil evolution were observed in more-stable areas under the natural forest or less disturbed areas. Clay lessivage through these soil profiles have led to formation of Typic or Calcic Haploxeralfs, while under croplands (CP) were classified as Typic Calcixerepts. Conversion of sloping deforested areas to CP along with inappropriate management have accelerated soil erosion, resulting in unstable conditions in which decalcification and formation of developed soils cannot occur. Paddy cultivation in flat areas has caused to reduced conditions and formation of Typic Haplaquepts. Because of unfavorable conditions for chemical weathering (e.g. lower water retention compared to more-stable areas) no vermiculite was detected in the CP. The results showed that evolution and classification of the studied soils were strongly affected by land-use type, topography and management.     

遥感与GIS的青海湖岸线分维研究

讨论了TM图像以及分形几何学对青海湖岸线问题的求解算法,同时估算了不同像元尺度下,青海湖的岸线长度得出青海湖岸线的分形维数为1.0237,并探讨了其地学意义,研究表明,分形维数不仅是一种可行的表达岸线特征的方法,而且岸线分维数的计算可大大减少工作量和提高工作效率,可见遥感与GIS技术在岸线分维数的计算中,具有十分良好的应用前景。     

四川盆地长宁构造地区龙马溪组页岩孔隙结构及其分形特征

以四川盆地长宁构造地区古生界下志留统龙马溪组页岩为例,利用低压氮气吸附测试手段和分形法理论,分析了页岩的孔隙结构参数及分形维数,结合有机质含量和矿物组成分析,研究了页岩孔隙结构特征及其分形特征的影响因素。结果表明:龙马溪组页岩孔隙结构复杂,孔隙主要以两端开口的圆筒状孔、墨水瓶状孔等开放性孔为主,孔径主要在1~10nm之间,孔径主峰位于1.86nm附近;页岩平均孔径在1.807~4.343nm之间,与w(TOC)、石英含量呈负相关,与黏土矿物含量呈正相关,其中w(TOC)对其影响显著;页岩总孔容在0.010 76~0.025 04cm3/g之间,比表面积在5.416~25.958m2/g之间,页岩总孔容、比表面积与w(TOC)、石英含量呈正相关,与黏土矿物、碳酸盐含量呈负相关;页岩具有双重分形特征,大孔隙分形维数D₁为2.091 8~2.653 7,小孔隙分形维数D₂为2.724 8~2.857 5,说明大孔隙结构的复杂程度大于小孔隙;页岩孔隙的分形维数与比表面积和总孔容呈正相关,而与平均孔径呈负相关;与w(TOC)、石英含量呈正相关,与黏土矿物、碳酸盐含量呈负相关,其中w(TOC)、石英和黏土矿物含量对页岩孔隙结构复杂性影响较大。      

Damage pattern recognition of spatially distributed slope damages and rainfall using optimal GIS mesh dimensions

Damage assessment for slopes using geographical information system （GIS） has been actively carried out by researchers working on several government organizations and research institutes in Korea. In this study, 596 slope damages were examined to identify the types of damage associated with dip angles, dip directions, and heavy rainfall resulting from typhoons in South Korea. Heavy rainfall of 100 mm to 300 mm resulted in 80% at the investigated slope damages. A GIS database was developed for highways, rainfall, soil or rock geometry, and types of damage. A grid of rainfall intensity was generated from the records of maximum daily rainfall. Contours for slope damages and heavy rainfall using optimal GIS mesh dimensions were generated to visualize damage patterns and show substantially strong correlation of rainfall with slope damages. The combination of remote sensing with the GIS pattern recognition process described in this work are being expanded for a new generation of emergency response and rapid decision support systems.     

Influence of principal stress rotation of unequal tensile and compressive stress amplitudes on characteristics of soft clay

Soil behavior can reflect the characteristics of principal stress rotation under dynamic wave and traffic loads. Unequal amplitudes of tensile and compressive stresses applied to soils have complex effects on foundation soils in comparison with the pure principal stress rotation path. A series of undrained cyclic hollow torsional shear tests were performed on typical remolded soft clay from the Hexi area of Nanjing, China. The main control parameters were the tensile and compressive stress amplitude ratio(α) and the cyclic dynamic stress ratio(η). It was found that the critical η tended to remain constant at 0.13, when the value of the compressive stress amplitude was higher than the tensile stress amplitude. However, the influence of the tensile stress was limited by the dynamic stress level when α= 1.For obvious structural change in the soil, the corresponding numbers of cyclic vibration cycles were found to be independent of α at low stress levels and were only related to η. Finally, a new method for evaluating the failure of remolded soft clay was presented. It considers the influence of the tensile and compressive stresses which caused by complex stress paths of the principal stress rotation. This criterion can distinguish stable, critical, and destructive states based on the pore-water-pressure-strain coupling curve while also providing a range of failure strain and vibration cycles. These results provide the theoretical support for systematic studies of principal stress rotation using constitutive models.