鄂尔多斯盆地子洲地区山2段咸水层CO_2埋存条件与潜力评价

以鄂尔多斯盆地子洲地区山2段咸水层为例,综合测井、岩心分析和薄片观察等资料,考虑CO_2埋存有效性和埋存稳定性,对试验区CO_2地质埋存可行性及埋存潜力进行评价;分析子洲地区山2—盒8段沉积相类型及时空演化规律,明确CO_2埋存地质体的储层岩石学、微观孔隙空间及储层物性分布特征,评价试验区盖层分布特征、断裂发育规律;结合试验区矿化度、水化学成分分析结果,认定试验区山2—盒8段具备注入CO_2并进行地质埋存的有利储盖条件。Z28-43典型井区的地质模型计算结果表明,试验区CO_2有效埋存量为2.48×10~6 t,为试验区后续的CO_2埋存研究奠定地质基础。     

青鳉foxl2基因敲除突变体的构建与表型分析

【目的】建立青鳉(Oryziaslatipes)foxl2基因突变体材料,为阐释foxl2基因功能提供良好的动物模型和研究基础。【方法】利用CRISPR/Cas9技术敲除青鳉foxl2基因,建立突变体材料。观察foxl2基因突变体的第二性征、性腺等形态特征;组织学分析性腺结构与性腺中细胞类型;用定量PCR检测性腺发育相关基因的表达情况。【结果】通过CRISPR/Cas9技术成功敲除青鳉foxl2基因,建立foxl2基因缺失4个碱基的青鳉突变体。从第二性征与性腺表型上,foxl2-/-突变体均表现为生理雄性;组织切片分析发现,foxl2-/-突变体的性腺结构与野生型卵巢和野生型精巢均有显著差异,存在退化的核仁外周卵母细胞与精子,提示foxl2-/-的性腺在组织结构上类似精巢;定量PCR结果显示,foxl2-/-突变体中雄性性腺发育关键基因sox9b、gsdf、amh等的表达量升高,雌性性腺发育关键基因cyp19a1a等的表达量下降。【结论】通过CRISPR/Cas9技术成功建立青鳉foxl2基因缺失的突变体,foxl2-/-突变体有遗传雌性、生理雄性的表型特征,foxl2对维持青鳉性腺功能有重要作用。     

Observation and simulation of 2-methylisoborneol in the Qingcaosha Reservoir,Changjiang estuary

Journal of Oceanology and Limnology - 2-Methylisoborneol (2-MIB) is a cyanobacterial metabolite that is responsible for many taste and odor (T&O) complaints related to the aesthetics of...     

Effect of Ca(OH)2, NaCl,and Na2SO4 on the corrosion and electrochemical behavior of rebar

The corrosion of rebar in reinforced concrete in marine environments causes significant damage to structures built in ocean environments. Studies on the process and mechanism of corrosion of rebar in the presence of multiple ions may help to control damage and predict the service life of reinforced concrete structures in such environments. The effect of interactions between sulfate and chloride ions and calcium hydroxide on the electrochemical behavior of rebar are also important for evaluation of structure durability. In this work, electrochemical impedance spectroscopy(EIS) plots of rebar in Ca(OH)_2 solution and cement grout, including NaCl and Na_2SO_4 as aggressive salts, were measured for different immersion times. The results show that corrosion of rebar was controlled by the rate of charge transfer as the rebar was exposed to chloride solution. In the presence of high concentrations of sulfate ions in the electrolyte, generation and dissolution of the passive film proceeded simultaneously and corrosion was mainly controlled by the diffusion rate. When Na_2SO_4 and NaCl were added to Ca(OH)_2 solution, the instantaneous corrosion rate decreased by a factor of 10 to 20 as a result of the higher p H of the corroding solution.     

The Influences of Mg~(2+), Ca~(2+) and Mg ~(2+)/Ca ~ (2+) Ratio in Mixed Seawater on the Emergence Rate of Penaeus japonicus Postlarva

ＩＮＴＲＯＤＵＣＴＩＯＮＰｅｎａｅｕｓｊａｐｏｎｉｃｕｓｉｓａｎｅｕｒｏｘｙｂｉｏｔｉｃｐｅｎａｅｉｄｐｒａｗｎ ,ｗｈｏｓｅｐｒｏｐｅｒｓａｌｉｎｉｔｙｒａｎｇｅｆｏｒｇｒｏｗｔｈｉｓ2 0 -30ａｎｄｏｐｔｉｍａｌｓａｌｉｎｉｔｙｒａｎｇｅｉｓ 2 5-30 (ＷａｎｇａｎｄＬｉｕ ,1 991 ;ＷａｎｇａｎｄＹｕ ,1 999) .Ｉｔｈａｓｇｒｅａｔｏｓｍｏｒｅｇｕｌａｔｉｏｒｙｃａｐａｃｉｔｙ (ＭａｎｔｅｌａｎｄＦａｍｅｒ,1 983) .Ｂｕｔｉｔｓｂｅｓｔｅｎｖｉｒｏｎｍｅｎｔｆｏｒｒｅｐｒｏｄｕｃｔｉｏｎａｎｄｄｅｖｅｌ…     

鲤JAK2基因分子克隆及组织表达分析

采用同源克隆策略和RACE-PCR技术,克隆得到可能的鲤(Cyprinus carpio)两面神激酶2(JAK2)基因的c DNA全长序列,包括3 378 bp的开放阅读框,732 bp的5'-非编码区,529 bp的3'-非编码区,总长度达4 639bp。开放阅读框可编码1 125个氨基酸,推测分子质量和理论等电点分别为129.33 ku和6.99。同源性分析显示,克隆的鲤鱼基因与斑马鱼(Danio rerio)JAK2同源性最高,其氨基酸序列同一性和相似度分别达89%和95%。结构域预测表明,所编码氨基酸序列包含FERM、SH2及两个酪氨酸激酶结构域,这4个结构域也保守地存在于其他脊椎动物的JAK分子中。高级结构预测显示,鲤JAK2主要包括α-螺旋(α-helix)、β-折叠(β-sheet)和连接环(loop)等3类结构元件。脊椎动物JAK分子系统进化树显示,JAK1、JAK2、JAK3和TYK2等4类JAK分子分别聚类,鲤JAK2处于JAK2支系中,与所有其他的鱼类JAK2聚为一大支,并与两栖类和哺乳类组成的另一大支构成姊妹群,表明它们具有共同的祖先基因,为直系同源关系。实时荧光定量PCR检测结果表明,鲤JAK2在皮肤中表达量最高,其次是肠、血液和脑,而在肌肉、鳃、头肾、心、肝、脾中表达量较低;鲤JAK3在脾中表达量最高,在其他组织中表达量均很低,甚至未检出。     

Absolute calibration of HY-2A and Jason-2 altimeters for sea surface height using GPS buoy in Qinglan,China

GPS buoy methodology is one of the main calibration methodologies for altimeter sea surface height calibration.This study introduces the results of the Qinglan calibration campaign for the HY-2 A and Jason-2 altimeters.It took place in two time slices;one was from August to September 2014,and the other was in July 2015.One GPS buoy and two GPS reference stations were used in this campaign.The GPS data were processed using the real-time kinematic(RTK)technique.The final error budget estimate when measuring the sea surface height(SSH)with a GPS buoy was better than 3.5 cm.Using the GPS buoy,the altimeter bias estimate was about-2.3 cm for the Jason-2 Geophysical Data Record(GDR)Version 'D' and from-53.5 cm to-75.6 cm for the HY-2 A Interim Geophysical Data Record(IGDR).The bias estimates for Jason-2 GDR-D are similar to the estimates from dedicated calibration sites such as the Harvest Platform,the Crete Site and the Bass Strait site.The bias estimates for HY-2 A IGDR agree well with the results from the Crete calibration site.The results for the HY-2 A altimeter bias estimated by the GPS buoy were verified by cross-calibration,and they agreed well with the results from the global analysis method.     

Effects of heavy metal ions (Cu2+, Pb2+ and Cd2+) on DNA damage of the gills, hemocytes and hepatopancreas of marine crab, Charybdis japonica

There are rising concerns about the hazardous effects of heavy metals on the environment. In this study, comet assay and DNA alkaline unwinding assay were conducted on the tissues (gills, hepatopancreas, and hemocytes) of Charybdis japonica in order to illustrate genotoxicity of three heavy metal ions (Cu2+, Pb2+, and Cd2+) on the marine crabs C. japonica. The crabs were exposed to Cu2+ (10, 50, and 100 ?g.L?1), Pb2+ (50, 250, and 500 ?g L?1) and Cd2+ (5, 25, and 50 ?g L?1), and the tissues were sampled at days 0.5, 1, 3, 6, 9, and 15. DNA alkaline unwinding assay was used for testing the DNA single strand break in gills and hepatopancreas and comet assay was employed for testing the DNA damage in hemocytes. The results showed that the DNA damage (F-value) of gills in the crabs exposed to the three heavy metals was decreased gradually during the exposure periods and there was a dose-time response relationship in certain time, suggesting that the levels of DNA single strand break in all the experimental groups increased significantly compared to the controls. Changes of F-value in hepatopancreas of the crabs exposed to the three heavy metals were similar to those in gills except that the peak values were found in the 500 ?g L?1 Pb2+ treatment group at day 3 and the 50 ?g L?1 Cd2+ treatment group at day 9. The ranks of DNA damage in gills and hepatopancreas induced by the three heavy metal ions (50 ?g L?1, day 15) were Cd2+ >Pb2+ >Cu2+ and Pb2+ >Cu2+ >Cd2+. The levels of DNA damage in gills were higher than those in hepatopancreas in the same experimental group. It can be concluded that indices of DNA damage can be used as the potential biomarkers of heavy metal pollution in marine environment.     

新疆阿克苏断层H2浓度影响因素及映震效能分析

利用相关和回归分析方法,分析断层H2浓度与气温、气压之间的相关关系,得到气温与断层H2浓度的回归方程.通过R值评分方法对断层H2浓度回归残差进行映震效能检验,结果表明:1)气温为阿克苏断层H2浓度变化的主要影响因素,与断层H2浓度呈高度正相关;气压对阿克苏断层H2浓度变化影响较小,与断层H2浓度呈中低度负相关.2)断层...     

Molecular identification,expression pattern,and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

Insulin-like growth factors (IGFs) are key regulators of development and growth. Here, we characterized the igf2 gene from olive flounder (Paralichthys olivaceus) and determined its temporal and spatial expression. We set up an in-vitro protein expression system in eukaryotic human embryonic kidney (HEK293T) cells and explored its effects on cell proliferation. The flounder igf2 cDNA contained a 648-bp open reading frame (ORF) encoding a protein of 215 amino acids (aa), which spanned the complete signal peptide (47 aa), mature peptide (70 aa), and E domain (98 aa). In adult flounder, igf2 mRNA was detected in all selected tissues. In early development, igf2 mRNA was detected throughout development from unfertilized eggs to hatching-stage embryos. In-situ hybridization analysis indicated that igf2 mRNA was specially expressed in the brain region, floor plate, hypochord, otic vesicle, and pectoral fin during embryogenesis. Western blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T cells. In addition, the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma (HeLa) and HEK293T cells. These results provide new information about the structural and functional conservation, expression patterns, and biological activity of the igf2 in teleosts.

     

FDTD(2M,2N)的一种实现方法

提出了实现高阶时域有限差分法FDTD（2M，2N）的一种方法，把对时间的高阶差分转化为对空间的差分，相对于FDTD（2，2N），并不增加存储量。数值实验证明了这一方法的有效性。     

FN5井区夏子街组异常高阻段成因及油气成藏主控因素

通过岩心、薄片、构造演化史、古地貌等对FN5井区二叠系夏子街组异常高阻段进行综合分析.结果表明:FN5井区二叠系夏子街组异常高阻段发育2种岩性,高阻致密隔层以粗粒级云化凝灰质砂砾岩为主;低阻储层段以细粒级凝灰质细(中细)砂岩为主,储集空间具裂缝—孔隙双重介质特征.垂向上主要分布在夏子街组中部的P2x22砂层组;平面上主要分布在扇三角洲前缘近岸坡折带以下FC1—F20—FN055—FN5—W27井一带,面积为6.2km2,平面形态呈孤立的土豆状.油藏类型为东南方向受乌南断裂控制、其余方向受岩性控制的构造—岩性油藏.其展布受扇三角洲前缘的近岸水下坡折控制,后期成藏受控于异常高阻段的厚度及距主控断裂乌南断裂的距离.具有油层垂向厚度大、单井产量高、储量丰度高特征.为乌尔禾地区二叠系2011年探明储量培植及2012年增储上产的主要层位.     

应用2H、18O同位素示踪华北平原石家庄包气带土壤水入渗补给及年补给量确定

采集2个不同深度包气带土壤水2H、18O同位素剖面ZK1,ZK2,应用天然稳定同位素2H、18O示踪的方法,研究了华北
山前冲积平原石家庄地区包气带土壤水入渗补给的历史演化特征。结果显示,研究区内,以0.05m 为取样间隔,δ2H、δ18O值可
以明显指示出大气降水及灌溉水入渗补给时间—剖面深度位置的年际对应关系。ZK2的δ2H、δ18O值随着埋深的增大出现周期
性的波动,具有分层现象的岩性差异并不明显,说明ZK2剖面以活塞流的入渗方式补给地下水。在0~3.90m深度,δ2H、δ18O值
显示降水和灌溉水的入渗补给时间为2011年10月至2001年11月。18O峰值位移法计算补给量的结果显示,降水、灌溉水通过包
气带补给地下水的垂向运移速度为38.5~65.0cm/a,年均入渗补给量为131.3~185.3mm。同时,对比2003年及2005-2007
年降水量数据,说明少雨年份农业灌溉用水量的大小对当地地下水的入渗补给量起着关键性作用。      

金坛岩盐卤水中Ca2+含量分析及处理方法

以陈家庄盐矿为研究对象,对原岩卤水中Ca2+含量的变化进行分析,同时也对用CaCl2为原料的精制卤水中Ca2+含量的变化进行了分析,从Ca2+在卤水中溶解度的角度出发,提出降低Ca2+含量(同时降低卤水中SO42-的含量)的几种方法.     

4种对虾耗氧率、CO_2排出量、呼吸商和窒息点的研究（英文）

较系统研究了斑节对虾、墨吉对虾、日本对虾和刀额新对虾等４种对虾的耗氧率、ＣＯ２排出量、呼吸商和窒息点。结果表明,同一种类不同大小和不同种类及生活习性不同,耗氧率、ＣＯ２排出量、呼吸商和窒息点有较大的差别。日本对虾、刀额新对虾的耗氧率、ＣＯ２排出量比其他两种虾低。墨吉对虾次之,斑节对虾为最高。对ＤＯ忍受能力,刀额新对虾最强,在ＤＯ０．６０ｍｇ／Ｌ才开始出现窒息。斑节对虾次之,在ＤＯ降至０．８８ｍｇ／Ｌ出现窒息。墨吉对虾在ＤＯ１．１９ｍｇ／Ｌ出现窒息。日本对虾忍受ＤＯ能力最差,在以ＤＯ１．３１ｍｇ／Ｌ出现窒息。     

鄂尔多斯盆地神木地区山2段、太原组天然气聚集条件研究

对神木地区山2段和太原组天然气聚集条件的分析发现,神木地区气源条件相对较好,生气强度整体大于16×108 m3/
km2;同时泥岩盖层封盖能力较好,山1段和山2段内的泥岩作为直接盖层,对其下伏地层中的砂岩气藏保存起到了重要作用。山
2段和太原组内储层整体较为致密,按其成岩作用与孔隙组合特征划分出4种类型的成岩相。在成岩相划分的基础上,考虑储层
宏观、微观非均质性和构造等多种因素的差异,确定了致密背景下优势聚集区的特征及其分布。在良好的气源基础上,不同优势
聚集区和泥岩的三维配套组合控制其内天然气的聚集程度,从而造成气藏呈现在三维空间内相邻或叠置的“准连续型”特征。      

高桥地区奥陶系马家沟组马五1-2亚段岩溶作用与孔隙演化

为了解鄂尔多斯盆地高桥地区奥陶系马家沟组马五1-2(Ma51-2)亚段岩溶作用与储层孔隙演化规律,采用岩心观察、铸
体薄片及扫描电镜、能谱等测试分析方法进行了研究。结果表明:研究区Ma51-2亚段碳酸盐岩经历了3期古岩溶作用:(准)同生
期岩溶、表生期岩溶和埋藏期岩溶。受加里东中、晚期—早海西期构造运动的影响,碳酸盐岩储层先后经历了准同生期成岩、表生
期成岩、浅埋藏成岩、中-深埋藏成岩阶段,相应地,孔隙演化经历了原生孔隙形成—缩减期、缝洞发育期、缝洞缩减充填期和裂
缝、埋藏溶蚀—充填期4个阶段。      

Parallel numerical simulation on CO2 geologic storage in Ordos Basin,China

The authors developed a 3-D numerical injection model on parallel TOUGH 2-FLAC3D based on the site-specific stratigraphic information in Ordos Basin to simulate the Shenhua CO2 geological storage ( CGS ) demonstration project injection process for three years and forecast CO2 migration and layers displacement after injection for seven years .The results suggested that CO2 migration did not stop after three years of consecutive injection, but migration rate was slowing down with time .As a result, displacement near injection well in-creased with injection and decreased slowly after injection .The maximum displacement of surface center oc-curred near the end of injection , which was only 1.24 cm.According to the displacement result , it is safe to continue injecting for this project .