Hydrocarbon-Degrading Bacteria and Paraffin from Polluted Seashores 9 Years after the Nakhodka Oil Spill in the Sea of Japan

Pollution of petroleum hydrocarbons, in particular oil spills, has attracted much attention in the past and recent decades. Oil spills influence natural microbial community, and physical and chemical properties of the affected sites. The biodegradation of hydrocarbons by microorganisms is one of the primary ways by which oil spill is eliminated from contaminated sites. One such spill was that of the Russian tanker the Nakhodka that spilled heavy oil into the Sea of Japan on January 2, 1997. The impact of the Nakhodka oil spill resulted in a viscous sticky fluid fouling the shores and affected natural ecosystems. This paper describes the weathering of hydrocarbon-degrading bacteria （genus Pseudomonas） and crystallized organic compounds from the Nakhodka oil spill-polluted seashores after nine years. The Nakhodka oil has hardened and formed crust of crystalline paraffin wax as shown by XRD analysis （0.422, 0.377, and 0.250 nm d-spacing） in association with graphite and calcite after 9 years of bioremediation. Anaerobic reverse side of the oil crust contained numerous coccus typed bacteria associated with halite. The finding of hydrocarbon-degrading bacteria and paraffin wax in the oil crust may have a significant effect on the weathering processes of the Nakhodka oil spill during the 9- year bioremediation.     

A social assessment of the Hebei-Spirit oil spill

This paper examines the early social consequences of the largest oil spill in Korea from December 2007 up to May 2008. The focus is on the social impact of the oil spill and its social implications in three major areas of oil spill response: (1) clean-up operations, (2) compensation, and (3) ecological impact. The paper highlights both the positive and negative consequences of the spill and the significance of the early social assessment to understand subsequent responses and adaptation in the long run.     

Response of the phoxocephalid amphipod,Rhepoxynius abronius,to a small oil spill in Yaquina Bay,Oregon

A spill of approximately 284,000 liters of Bunker C and diesel fuel oils occurred at the entrance of Yaquina Bay, Oregon, following the wreck of the freighterBlue Magpie on 19 November 1983. A portion of this oil entered the lower estuary and was deposited on subtidal benthic habitats occupied by the phoxocephalid amphipodRhepoxynius abronius. This species is particularly sensitive to contaminants in sediment and its life history had previously been studied at the same sites affected by the spill. The oil was initially present as small, sand-coated globules at the study site, and persisted in association with detritus and sediment for months. Bioassays withRhepoxynius abronius showed that the oil globules were not acutely toxic unless mixed into the sediment at concentrations of 1.0 parts per thousand or greater. A series of 10-d bioassays before and after the spill showed that sediment collected from oiled subtidal sites did not become acutely toxic to this species. Although the density of theR. abronius population declined by 75% after the spill, similar declines of the same population were observed at this site in fall 1980. Oil-exposedR. abronius from Yaquina Bay were slightly more sensitive to cadmium in sediment than individuals from Whidbey Island, Puget Sound, Washington. Although mean fecundity was greater in 1984 than in 1981, recruitment following the spill was lower than in the 1980–1981 study. Thus, there is limited evidence for a small impact of the oil spill on this sensitive amphipod.     

Application of an oil spill vulnerability index to the shoreline of lower Cook Inlet,Alaska

Offshore tracts in Alaska's lower Cook Inlet are scheduled to be opened for exploratory petroleum drilling in the near future. Because of the potential for oil spills resulting from this activity, a field study of the coastal zone was conducted in June 1976. A total of 1216 km of shoreline was classified into 3 types: erosional (45 percent), neutral (38 percent), and depositional (17 percent). These were further subdivided into 16 subclasses on the basis of small scale morphological features. This classification was used in conjunction with a vulnerability index of potential oil spill damage, developed through study of three major oil spills, to predict the longevity of oil in the different coastal environments of the Inlet. On a scale from 1 to 10, 45 percent of the shoreline has low values of 1 to 4, which means that oil would be dispersed by natural processes within less than six months after a spill on these coasts. Values from 4 to 6 were assigned to 13.4 percent of the shoreline, where oil residence time may be up to one year. A 6 to 10 rating was assigned to 41.5 percent of the shoreline, where oil contamination may remain for periods of two to ten years, or possibly longer should no major clean-up procedures be initiated. We propose that the use of this type of vulnerability indexing, in conjunction with a biological susceptibility index and oil spill trajectory models, would provide a rational basis for decision making concerning the location of on- and off-shore oil facilities and the design of oil spill contingency plans.     

Tracing subsurface oil pollution leakage using 2D electrical resistivity tomography

This study provides an evaluation of the subsurface oil contaminant flow in an oil production field at the Wadi Feiran fan, along the Gulf of Suez, Egypt. The 2D electrical resistivity tomography technique has been applied along four profiles surrounding the spill location of oil dump site. Theoretical soil resistivity response versus fluid resistivity for different cation exchange capacity values has been studied. Then, a physical model for the current situation of flowing the oil over the groundwater (sea invaded water) of saturated fluvial sediments has been introduced. It was supposed that the crude oil has higher resistivity values and turned to be conductive far away from the oil spill source under the effect of microbial biodegradation. Such conditions of contaminated distribution and its resistivity values have been considered during the inversion and interpretation of the acquired data sets. The processed 2D resistivity profiles reveal that oil plumes are accumulated in the seawater invasion direction, whereas it has low signatures along the profiles parallel to the shore line.     

Impacts of the ‘<Emphasis Type="Italic">Pacific Adventurer</Emphasis>’ Oil Spill on the Macrobenthos of Subtropical Sandy Beaches

Biological impacts of oil on sandy shore ecosystems remain incompletely understood, especially following smaller spills on subtropical beaches. Here we quantified changes to benthic assemblages on a sandy beach following the 270-t spill of heavy fuel oil from the Pacific Adventurer that occurred in March 2009 off Moreton Island in Eastern Australia. Assessments of ecological impacts are based on spatial contrasts between multiple reference and impact sites sampled 1 week and 3 months after the spill. Benthic invertebrate assemblages exposed to oil had significantly fewer individuals of fewer species 1 week after the spill, markedly changing their ecological structure. Biological differences consistent with oil-related mortality were significant on the lower shore and in the swash and remained so 3 months after the spill. This signals a lack of recovery of these communities in the short term, despite the fairly rapid removal of oil. Results show that, despite the relatively small size of the spill, heavy fuel oil contamination can cause substantial impacts on sandy beach ecosystems, and that recovery may be prolonged.     

Multimedia environmental analysis of PCBs fate and transport mechanism through a case study of transformer oil leakage

Transformer oil leakage having large quantity of PCBs is one of the most deplorable incidents resulting in huge negative impacts on the environment. In this paper, two different models, i.e. CHEMCAN and the hydrocarbon spill screening models, were applied to the case study adapted from a real case of PCBs in transformer oil spill in Montreal. The oil migration was examined in three dimensions including site-specific data, soil characteristics and hydrogeological properties. This study aims to investigate the fate, transport and transformation rates of PCB in the soil, the unsaturated and the saturated zones. The proposed modelling concept helps to define the processes to be used in characterization of soil and subsurface environment as a receptor of transformer oil spill. This study includes the simulation techniques for assessing the effect of PCBs on groundwater and soil. Results from multimedia environmental model and HSSM model verify with each other, and both show that 92.7 % of PCBs were found in soil as compared to groundwater. Considering decay and sorption processes at spill location, concentration of PCBs in soil was >50 mg/kg. This study can serve as a basis for further analysis and assist in selection of remediation technique.     

输油管道建设对鄂尔多斯盆地地下水影响预测

探讨输油管道建设对鄂尔多斯盆地地下水环境的影响,根据研究区地质及水文地质特征,结合石油管道事故所设定条件,按照风险最大化原则,采用“瞬时注入示踪剂——平面瞬时点源”模型进行预测分析,结果表明：污染物在地下水中运移的1460d内,最大迁移距离呈先增大后减小的趋势,最大距离为116．05m;中心点浓度则从433．13mg／L减少至〈0．3mg／L,低于《生活饮用水卫生标准（GB5749—2006）》中石油类限值（0．3mg／L）;泄漏中心点逐渐运移至下游49．64m处,对地下水敏感点不会产生明显影响。     

Sublethal Effects of Crude Oil on the Community Structure of Estuarine Phytoplankton

While the ecological impacts of crude oil exposure have been widely studied, its sublethal effects on phytoplankton community structure in salt marsh estuaries have not been well documented. The purpose of this study was to simulate oil spill conditions using a microcosm design to examine short-term (2 day) changes in phytoplankton community composition and total biomass following exposure to crude oil obtained from the Deepwater Horizon oil spill and a mixture of Texas crude oils. Microcosm experiments were performed in situ in North Inlet Estuary near Georgetown, SC. A control and six replicated experimental treatments of crude oil additions at final concentrations of 10, 50, or 100 μl l⁻¹ of either Deepwater Horizon spill oil or the Texas crude mixture were incubated under in situ conditions. Photopigments were analyzed using high-performance liquid chromatography and community composition was determined using ChemTax. Total phytoplankton biomass (as chl a) declined with increasing crude oil concentrations. Prasinophytes, the most abundant microalga in both experiments, showed no response to oil exposure in one experiment and a significant negative response in the other. Diatoms euglenophytes and chlorophytes appeared relatively resistant to oil contamination at the exposure levels used in this study, maintaining or increasing their relative abundance with increasing oil concentrations. Chlorophytes and cyanobacteria increased in relative abundance while cryptophyte abundance decreased with increasing oil concentrations. The results of these experiments suggest that low levels of crude oil exposure may reduce total biomass and alter phytoplankton community composition with possible cascade effects at higher trophic levels in salt marsh estuaries.     

Numerical modeling of two-phase fluid flow and oil slick transport in estuarine water

Oil spills is one of the most important hazards in the estuarine and coastal water. In recent decades, engineers try to predict the status of oil slick to manage the pollution spreading. The prediction of oil slick transport is carried out mainly by means of numerical models. In the current study, the development and application of a two-phase fluid flow model to simulate oil transport in the marine environment are presented. Different transport and fate processes are included in the developed model. The model consists of the Lagrangian method for the advection process, the Random Walk technique for horizontal diffusion process and the empirical equations for the fate processes. The major forces for driving oil particles are fluid current, wind speed and turbulent flow. Therefore, the multi-component hydrocarbon method has been included to the developed model in order to predict fate processes. As prediction of particle velocity components is of major importance for oil slick advection, therefore the binomial interpolation procedure has been chosen for the particle velocity components computations. In addition, shoreline boundary condition is included in the developed model to simulate shore response to oil slick transport near the beaches. The results of the model applications are compared with the analytical solutions, experimental measurements and other numerical models cited in literature. Comparisons of different sets of results represent the capability of developed model to predict the oil slick transport. In addition, the developed model is tested for two oil spill cases in the Persian Gulf.     

Coupled solution of oil slick and depth averaged tidal currents on three-dimensional geometry of Persian Gulf

In this paper, simulation of oil spill due to tidal currents in Persian Gulf is performed by coupled solution of the hydrodynamics equations and an equation for convection and diffusion of the oil. The hydrodynamic equations utilized in this work consist of depth average equations of continuity and motion in two dimensional horizontal planes. The effect of evaporation is considered in the continuity equation and the effects of bed slope and friction, as well as the Coriolis effects are considered in two equations of motion. The overlapping cell vertex finite volume method is applied for solving the governing equations on triangular unstructured meshes. Using unstructured meshes provides great flexibility for modeling the flow in arbitrary and complex geometries, such as Persian Gulf flow domain. The results of the hydrodynamic model for tidal currents in Persian Gulf domain is examined by imposing tidal fluctuations to the main flow boundary during a limited period of time. Finally, the developed model is used to simulate an accidental oil spill from a point in Persian Gulf.     

<Emphasis Type="Italic">Deepwater Horizon</Emphasis> Oil Spill Impacts on Salt Marsh Fiddler Crabs (<Emphasis Type="Italic">Uca</Emphasis> spp.)

The Deepwater Horizon oil spill was the largest marine oil spill in US waters to date and one of the largest worldwide. Impacts of this spill on salt marsh vegetation have been well documented, although impacts on marsh macroinvertebrates have received less attention. To examine impacts of the oil spill on an important marsh invertebrate and ecosystem engineer, we conducted a meta-analysis on fiddler crabs (Uca spp.) using published sources and newly available Natural Resources Damage Assessment (NRDA) and Gulf of Mexico Research Initiative (GoMRI) data. Fiddler crabs influence marsh ecosystem structure and function through their burrowing and feeding activities and are key prey for a number of marsh and estuarine predators. We tested the hypothesis that the spill affected fiddler crab burrow density (crab abundance), burrow diameter (crab size), and crab species composition. Averaged across multiple studies, sites, and years, our synthesis revealed a negative effect of oiling on all three metrics. Burrow densities were reduced by 39 % in oiled sites, with impacts and incomplete recovery observed over 2010–2014. Burrow diameters were reduced from 2010 to 2011, but appeared to have recovered by 2012. Fiddler crab species composition was altered through at least 2013 and only returned to reference conditions where marsh vegetation recovered, via restoration planting in one case. Given the spatial and temporal extent of data analyzed, this synthesis provides compelling evidence that the Deepwater Horizon spill suppressed populations of fiddler crabs in oiled marshes, likely affecting other ecosystem attributes, including marsh productivity, marsh soil characteristics, and associated predators.     

生物降解作用对烷基萘异构体分布的影响及其控制因素

在受到溢油污染的海滩上采集不同时间序列的石油样品,对芳烃组分烷基萘中的三甲基萘和四甲基萘各异构体的生物降解过程响应进行了详细分析。研究揭示,三甲基萘比四甲基萘更易被生物降解,其中1,2,7-三甲基萘、2,3,6-三甲基萘、2,3,6,7-四甲基萘、1,2,3,7-四甲基萘和1,3,5,7-四甲基萘等异构体具有相对较高的生物降解效率,与最近 Ostojié等利用理论模型计算所给出的降解序列十分一致,进一步证实了烷基萘在表生环境中的降解主要受控于异构体萘环上取代基的数量和位置。研究结果对于表生环境中萘系物类多环芳烃污染的生物修复评价具有重要指导意义。     

Real-time application of an oil spill movement prediction system

This paper deals with the prediction, in real time, of the motion of experimental oil slicks. The experiments were conducted during September 1983 offshore near Halifax on the east coast of Canada. The objectives of the experiments were (i) to determine the suitability of oil spill dispersants as countermeasures and (ii) the testing and verification of oil spill trajectory models and systems.The Atmospheric Environment Service (AES) participated in the experiments to test the capability of its oil spill motion prediction system in providing real-time trajectory forecasts. The experiment consisted of three sets of spills. Each set had a control slick and a test slick. Sixteen barrels of crude oil were used in each spill. The test slicks were used to test the effectiveness of various dispersants, the control slicks were used to verify trajectory forecasts.The spill trajectories and oil weathering information obtained from the system during the experiments demonstrated the relative ease with which the system could handle the required input and provide timely forecasts. The accuracy of these forecast trajectories was confirmed by observations, and their utility was demonstrated by their application in the operational decision-making process.     

Equilibrium sorption of crude oil by expanded perlite using different adsorption isotherms at 298.15 k

During the past decades, a significant increase occurred in accidental oil spill in the aquatic environments. In this regard, oil spill in Marine freshwater is still considered as a major environmental hazard. In this research, the experimental data on the sorption capacity of expanded perlite to crude oil were correlated with the equilibrium isotherm of Langmuir, Freudlich, Tempkin and the three parameter Redlich-Peterson isotherms. The results obtained from each specified isotherms were compared and accuracy of the models were favorably discussed. Accuracy of each model using the error function were evaluated. Moreover, the effect of type of objective function on the final results was investigated. To bring up the idea; the sum of square of the average squares of the errors, the sum of the squares of the errors, the hybrid fractional error function, Marquardt’s percent standard deviation and Chi-Square objective function were used and the accuracy was obtained using each objective function. The results showed that the Redlich-Peterson model can better represent the equilibrium isotherm data for the crude oil to be up taken on the expanded perlite.     

An assessment on oil spill trajectory prediction: Case study on oil spill off Ennore Port

A Liquefied Petroleum Gas (LPG) tanker and a chemical tanker collided two nautical miles off Ennore port on 28 January, 2017. Around 196.4 metric tons (MT) of Heavy Furnace Oil (HFO) was spilled and drifted towards the shore. Oil spill drift advisory and prediction was made by Indian National Centre for Ocean Information Services (INCOIS) using General National Oceanic and Atmospheric Administration (NOAA) Operational Modeling Environment (GNOME), an oil spill trajectory model. The trajectory model was forced with analysed and forecasted ocean currents from Global Ocean Data Assimilation System (GODAS) based on Modular Ocean Model 4p1 (GM4p1). It was found that spread of HFO obtained from oil spill trajectory model GNOME, has matched well with the observed spread from Sentinel-1A satellite dataset. However, the spread of the HFO was underestimated by the trajectory model, when forced with forecasted GM4p1 currents. Additional ground truth observation from Indian Coast Guard also corroborates this finding.     

基于数学统计法的海岸带溢油指纹鉴定研究：以大连石油污染事件为例

基于数学统计模式对溢油的指纹进行数字化识别对实现快速、准确进行溢油鉴别具有重要意义。介绍了重复性限法、聚类分析法和t检验法等多元统计分析方法进行溢油鉴别的方法、原理及鉴别原则;并以大连“七一六”输油管道爆炸事故为典型溢油案例,采用重复性限法对溢油鉴别常规诊断比值进行评价和筛选,并利用聚类分析法和t检验法对大连金沙滩和海贝广场附近出现的油污进行了污染源的有效鉴定。     

Trace analysis of hydrocarbons in coral cores from Saudi Arabia

As part of a long-term environmental assessment of the impact of the 1991 Gulf War on coral reefs, the Australian Institute of Marine Science (AIMS) conducted growth and chemistry studies on coral cores from the Arabian Gulf. Twenty-eight cores were collected from four coral reefs located offshore from Saudi Arabia.Annual coral growth bands surrounding the 1991 oil spill were analysed in selected cores. Additionally, in cores that extended to the early 1980s, annual layers from 1980 to 1986 were analysed for possible residues from the 1983–4 oil spill caused by the Nowruz oil field blowout during the Iraq–Iran war. Both major spill events were targeted to provide additional confidence in relating oil concentrations to specific pollution events. We detected petroleum biomarkers in several coral annual bands related to the major pollution events. However, the oil remaining in these cores has been altered over time and the biomarker ratios found in these oil residues differed from Gulf crude oils.The concentrations of hydrocarbons were compared with the growth parameters of the coral cores. Only one sample may have recorded an “impact” of oil exposure. There was no other correspondence between slight changes in growth parameters between years and the trace chemistry. Therefore, chemical analysis enabled detection of the exposure incident, but provided no insight into the amount of oil to which the coral had been exposed. The chemical data can only infer biological impact if growth was significantly decreased.The average coral growth characteristics of Porites from the four sites in the Arabian Gulf were normal, as predicted from the average water temperatures of the region. There was an indication of an overall decline in growth over time, which should be monitored in the future.     

The influence of capillarity on multiphase flow within porous media: a new model for interpreting fluid levels in groundwater monitoring wells in dynamic aquifers

A new approach is presented to calculate the volume of oil in the underground at an oil spill site from fluid levels in monitoring wells. The approach includes the effects of hysteresis due to irregular pore geometry and to phase entrapment. It is possible to explain the drastic changes in the oil thickness in a monitoring well due to the decrease and increase in the groundwater table. A correct evaluation of the oil volume infiltrated underground from an oil spill and the effective control of remediation works can only be done by using the newly developed approach with a consideration of the dynamic changes in the groundwater table.     

Numerical modeling of stress and strain associated with the bending of an oil pipeline by a migrating pingo in the permafrost region of the northern Tibetan Plateau

Small seasonal pingos formed in Quaternary deposits along active fault zones in permafrost of the northern Tibetan Plateau exert destructive forces to oil pipelines, bridges, culverts and other engineering facilities along the Golmud–Lhasa railway and highway. The pingos are particularly hazardous as they change position, or migrate, nearly every year. Three-dimensional finite element modeling reveals the enormous force from exerted by a pingo at the 86th station of the highway. A good representation of the stress and strain fields resulting from an expansion of a pingo and bending of an oil pipeline at the station are calculated after due consideration of the interaction between permafrost, pingo and pipeline. This followed establishing an engineering-geologic model from the field data and determining the mechanical properties of the media from field and laboratory tests. The maximum, intermediate and the minimal principal compressive stresses are calculated as well as those for the plastic strain. Concentrations of principal stress and plastic strain occur beneath the pipeline bend and both the principal compressive stress and resulting plastic strain become very small away from the pingo. Also, the bottom of the pingo is dominated by minimal values of principal stress and strain and the potential bending of a buried pipe caused by an expansion of a pingo is indicated to decrease as depth of burial increases.The pingo growth at the 86th station resulted in the bending upward of a 20m section of a buried oil pipeline, but it did not break and spill oil. Analysis of the pipe within the bend found the maximum, intermediate, and minimal principal compressive stress ranges that leads to plastic strain within the bent pipe. Compressive stress and plastic strain concentrations form in the inner sides of inflexions in the pipe bend, and tensional stress and plastic strain concentrations form in their outer sides where stress exceeds the yield limit of the pipe, but many irregularities are present. Such numerical modeling of stress and strain may offer key parameters for designing oil pipelines and engineered facilities to decrease the hazard from migrating pingos in similar geologic settings in the permafrost of the northern Tibetan Plateau.