Untersuchungen zum Nachweis von Escherichia coli im Wasser

From river water there were isolated 21 species (mainly coliform bacteria) in 914 strains. Of the pure cultures of coliform bacteria (E. coli, Enterobacter, Klebsiella, Citrobacter) 74.1 % of E. coli and 23.2 % of the Klebsiella strains showed lactose decomposition with gas formation at 44 °C. More than 90 % of the isolated Klebsiella did not form indole. Therefore, the temperature test and the indole formation (at 44 °C) (TI-test) made a very specific detection of E. coli in water possible. 94.7 % of the cultures grown initially on endoagar after a positive TI-test proved to be E. coli. The detection of E. coli showed a very good agreement with the standard method and the TI-test, the detection with the standard method being considerably dependent on the number of checked coliform colonies.     

Zum Einfluß von Rückständen von Zink- und Bleiverbindungen im Wasser auf die Assimilation von Fontinalis antipyretica

The carbon-dioxide assimilation of Fontinalis antipyretica is inhibited by 100 mg/1Zn²⁺ or Pb²⁺, without a lethal effect being caused. With increasing time of exposure from one to six days the threshold value of toxicity is lowered and at the same time the net assimilation is reduced within the range of concentration of 0.1 mg/1. In the ecological optimum of the environmental conditions the toxicity is weaker than under other unfavourable environmental conditions at the same time. Therefore, the photoecologically optimum illumination of 1200 lx is an effective bioprotector against phytotoxic lead ion concentrations, so that at 1200 lx also at a comparatively high load a positive photosynthesis balance can be maintained. In the dark, in the first hour after the application of the lead ions dissimilation is a function of concentration, subsequently the respiration differences decrease very much in the range between 0 and 100 mg/1 lead(II)-ions. Stimulating effects could be demonstrated only by 0.01 mg/1 Pb²⁺ and only for the carbondioxide assimilation.     

Einfluß der TTC-Konzentration auf die Dehydrogenasenaktivität von Mikroorganismen

Die aus dem Sediment eines Gewässers isolierte Mischpopulation von Bakterien wurde auf Nährbouillon kultiviert sowie über unterschiedlich lange Zeit an Glucose und Phenol adaptiert. Die zur Bestimmung der Dehydrogenasenaktivität optimale Konzentration des TTC lag für die verschiedenen Kulturen zwischen 0,04 und 0,2 Volumenprozent der Bakterien-Suspension bzw. zwischen 0,1 bzw. 1 mg TTC/mg TS · h bezogen auf die getrocknete Bakterienbiomasse bei einstündiger Inkubation. Die Unterschiede in der Formazanbildung wie in der toxischen Wirkung des TTC sind abhängig von der Struktur der einzelnen Bakterienzelle wie von der Struktur der Belebtschlammflocken und dem ,,Alter“ der Kultur, weniger abhängig vom Substrat.     

Untersuchungen zum Vorkommen von R+-Bakterien im Flußwasser

With colony numbers of 2 · 10⁴… 3 · 10⁵ and coliform contents of 2 · 10³… 1 · 1 · 10⁴ per ml, in the investigated river section 2.1% of colonies were resistant to tetracycline, 0.3% to chloramphenicol, 7.5% to kanamycin, 5.4% to trimethoprim, 12.7% to ampicillin and 0.14% to gentamycin. For the coliforms the resistances were 5.1% to tetracycline, 2.1% to chloramphenicol, 2.7% to kanamycin and 0.15% to gentamycin. There were isolated 63 different resistance types. Of the resistant Klebsiella strains 75% were resistant to three or more antibiotics. Of the coliform bacteria (Escherichia coli, Klebsiella, Enterobacter, Citrobacter) 31.6… 51.9% of the strains transferred their antibiotics-resistance to Escherichia coli K-12, for the antibiotics-resistant strains of Pseudomonas and Aeromonas a transfer of resistance was not observed at all.     

Experimental Approach to Cadmium Effects on a Marine Protozoan Community Untersuchungen zum Einfluß von Cadmium auf eine marine Protozoen-Gemeinschaft

The effects of cadmium on a marine protozoan community were studied. After 96 h (0.5 mg/L cadmium) there was a reduction of biomass and a dissappearance of bacterivorous ciliates that were replaced by small flagellates. Other cadmium concentrations utilised affected abundance and biomass after 48 hours. A change of the community structure by the loss of Cd-sensible species and the appearance of Cd-resistant species occurred. The structure of community was simplified and the diversity and variety values decreased. The bacterivore-detritivore and photoautotroph groups, that were the main components in the control, were notably reduced in the presence of cadmium. The metal concentrations on the protozoan biomass increased during the experiment, indicating a certain bioassimilative role of these organisms.     

Einfluß der Temperatur auf die Bildung von Magnetit als Zusatzstoff für die Magnetseparation

Influence of the Temperature on the Formation of Magnetite as an Additive for Magnetic Separation To investigate the influence of the temperature on the formation of magnetite in aqueous solution, experiments were performed in which ferrous hydroxide was precipitated from oxygen-free ferrous sulfate solution (c₀(Fe) = 5 mmol·dm^?3) and subsequently oxidized with synthetic air. The precipitates so obtained are a mixture of solids whose compositions depend on the temperature and on the rate of aeration. Above all, magnetite, goethite, and δ-FeOOH had been formed. Magnetite occurs at temperatures above about 15 °C, goethite above 35 °C, and δ-FeOOH below about 10 °C. Referring to the thermogravimetric plots and to the relationship between the temperature of formation and the pH measured upon completion of the reaction, it is justified to assume that also sulfate is contained in the precipitates. The values of the magnetic moment of the precipitates, which are crucial in magnetic separation, made evident the dependence on the temperature of formation expected on the basis of solid analyses and confirmed the presence of δ-FeOOH at rather low temperatures. At the rates of aeration applied, it was possible to obtain from about 15 °C on solids having magnetic moments which are sufficient for magnetic separation.     

Der Einfluß von Erdölkohlenwasserstoffen auf die Primärproduktion des Donauplanktons

Danube river water samples were saturated with mineral oil, and then the primary production (gross) was determined by means of the light-dark bottle method (oxygen) in situ at depths of 0.1… 1.5 m in comparison with untreated samples. Samples were exposed for half a solar day alternately during the first and second half-days. Investigations carried out between March and October for periods of 14 days showed a mean production of 2.56 g · m^?2d^?1 O₂, which was reduced by 36% due to 12.6 mg/l hydrocarbons. Production decreases with the water depth from 6.2 to 0.9 mg · l^?1d^?1 0₂, whereas the inhibition by hydrocarbons increases from 31 to 41%.     

Einflußfaktoren auf die Freisetzung von DOC und AOX unter ehemaligen Abwasserversickerungsflächen

Factors Influencing the Release of DOC and AOX out of Former Wastewater Infiltration Soils The influence of soil and of infiltration water quality on the release of dissolved organic carbon (DOC) and adsorbable organic halogens (AOX) from two former wastewater infiltration sites was investigated in laboratory column studies. Desorption was the most important factor influencing release processes. It depends on the amount of sorbent and sorbate and the strength of binding. Therefore, for constant irrigation rates a higher soil organic carbon content coincided with higher DOC-contents of the column effluent, while the irrigation water quality was of minor importance. Within one system the dependencies of AOX release and DOC mobilization were found to be similar. Transferring these dependencies on other systems has its limitations because of a different binding quality between sorbent and sorbate.     

Effects of Deicing-Salts on Heavy Metal Mobility Zum Einfluß von Streusalzen auf die Beweglichkeit von Schwermetallen

Heavy metal input into roadside soils and effects of deicing salts are often discussed as separate environmental problems. However, a relationship between salt input and heavy metal mobility has already been detected in laboratory tests. During a 2-year field study, the soil solution of a roadside soil was continuously sampled. The results of soil solution analysis not only showed a strong rise in deicing-salt related sodium, calcium and chloride concentrations, but also high zinc and cadmium values in winter and spring. Additional laboratory tests confirmed the mobilizing effects of salt solution which contained NaCl and CaCl₂. Cation exchange and the formation of chlorocomplexes are discussed as the responsible mechanisms.     

Überleben von Campylobacter jejuni im Wasser

The Campylobacter jejuni survival pattern was investigated in model experiments using drinking water and surface waters differing in organic contamination. Further conditions studied were two different temperature levels (4 °C and 20 °C, resp.) as well as several starting densities of the testing strain. In drinking water, i.e. relatively uncontaminated water, the starting density showed a direct influence on the interval of surviving. The same was noted for temperature, where higher levels (20 °C) resulted in a relative quick inactivation of the investigated agent. Mentioned influences played a similar role in the surface water medium, together with the level of organic contamination: An increase of the value of COD to about 15.4 mg/l O₂ halved the interval of surviving. No relation to the dissolved oxygen tension was found.