Aspartic acid racemization dating of Holocene brachiopods and bivalves from the southern Brazilian shelf, South Atlantic

The extent of racemization of aspartic acid (Asp) has been used to estimate the ages of 9 shells of the epifaunal calcitic brachiopod Bouchardia rosea and 9 shells of the infaunal aragonitic bivalve Semele casali. Both taxa were collected concurrently from the same sites at depths of 10 m and 30 m off the coast of Brazil. Asp D/L values show an excellent correlation with radiocarbon age at both sites and for both taxa (r²_{Site 9 B. rosea} = 0.97, r²_{Site 1 B. rosea} = 0.997, r²_{Site 9 S. casali} = 0.9998, r²_{Site 1 S. casali} = 0.93). The Asp ratios plotted against reservoir-corrected AMS radiocarbon ages over the time span of multiple millennia can thus be used to develop reliable and precise geochronologies not only for aragonitic mollusks (widely used for dating previously), but also for calcitic brachiopods. At each collection site, Bouchardia specimens display consistently higher D/L values than specimens of Semele. Thermal differences between sites are also notable and in agreement with theoretical expectations, as extents of racemization for both taxa are greater at the warmer, shallower site than at the cooler, deeper one. In late Holocene marine settings, concurrent time series of aragonitic and calcitic shells can be assembled using Asp racemization dating, and parallel multi-centennial to multi-millennial records can be developed simultaneously for multiple biomineral systems.     

Kinetic model options for interpretation of amino acid enantiomeric ratios in Quaternary mollusks: Comments on a paper by Kvenvoldenet al. (1979)

A comparison of linear (to D/L leucine of ca. 0.55) and non-linear (slowing of racemization rates by a factor of ca. 10 × after D/L leucine of ca. 0.25) model kinetics is presented in order to interpret amino acid enantiomeric ratio data in Holocene and Pleistocene marine mollusks from the Pacific coast of the United States. Linear model rate constants for well-dated Holocene and Pleistocene samples are not consistent with probable temperature histories or probable latitudinal differences in effective temperatures for these samples, using reasonable values for the activation energy of racemization. The non-linear model, with a much earlier transition from rapid to slow racemization rates, appears more consistent with available data.     

Assessment of integrity and geochronology of archaeological sites using amino acid racemization in land snail shells: Examples from central Texas

Detailed racemization analyses were carried out on samples of the land snail Rabdotus mooreanus from archaeological sites at Fort Hood, in central Texas. D -alloisoleucine/L -isoleucine (A/I) values were determined for 260 individual shells from 29 proveniences, including sites in alluvium, colluvium, and rockshelters, as well as burned rock middens. A/I values show a good correlation with radiocarbon age, and so provide reasonably precise estimates of ages. Analyses indicate the presence of redeposited material in a large number of proveniences. These result from sedimentary processes involved in burial of the sites as well as from later disturbance (aboriginal or recent) of site stratigraphy. Because amino acid racemization analyses are relatively easy to carry out, this method lends itself to very detailed chronostratigraphic analyses of archaeological sites, thus permitting assessment of site integrity and assisting in the interpretation of site formation processes. © 1996 John Wiley & Sons, Inc.     

Late Holocene radiocarbon and aspartic acid racemization dating of deep-sea octocorals

Primnoa resedaeformis is a deep-sea gorgonian coral with a two-part skeleton of calcite and gorgonin (a fibrillar protein), potentially containing long-term records of valuable paleo-environmental information. For various reasons, both radiocarbon and U/Th dating of these corals is problematic over the last few centuries. This paper explores aspartic acid racemization dating of the gorgonin fraction in modern and fossil specimens collected from the NW Atlantic Ocean. Radiocarbon dating of the fossil specimen indicates a lifespan of 700 ± 100 years, the longest yet documented for any octocoral. Gorgonin amino acid compositions were identical in the fossil and modern specimens, indicating resistance to organic diagenesis. Similar to bone collagen, the fibrillar protein of gorgonin may impose conformational constraints on the racemization of Asp at low temperatures. The rate of racemization of aspartic acid (d/l-Asp) was similar to previously published results from an 1800 year old anemone (Gerardia). The age equation was: age (years BP 2000 AD) = [(d/l − 0.020 (±.002))/.0011 (±.0001)]² (r² = 0.97, p < .001). The error in an age estimate calculated by d/l-Asp was marginally better than that for ¹⁴C dating over the most recent 50-200 years, although the dating error may be improved by inclusion of more samples over a broader time range. These results suggest that d/l-Asp dating may be useful in augmenting ¹⁴C dating in cases where ¹⁴C calibrations yield two or more intercept ages, or in screening samples for further ¹⁴C or U/Th dating.     

Stratigraphy of late Quaternary estuarine deposits and amino acid stereochemistry of oyster shells beneath San Francisco Bay,California

The sequence of Quaternary deposits beneath the floor of San Francisco Bay includes four to seven noncontemporaneous estuarine units intercalated with alluvium and dune sand. Units L (0–10,000 B.P.), M (>40,000 B.P., probably ca. 80,000–140,000 B.P.), and N (older than unit M) are distinctly superposed. The dominant molluscan fossil in each of these three units is Ostrea lurida Carpenter, the native oyster along much of the pacific Coast of North America. Despite a lamellar structure that suggests vulnerability to contamination, O. lurida shells generally yield amino acid enantiomeric ratios that are analytically reproducible and stratigraphically consistent. The kinetics of racemization in O. lurida conceivably resembles that of Protothaca and Saxidomus, other bivalves whose kinetics of racemization are relatively well understood. Assuming such a resemblance, enantiomeric ratios in O. lurida imply that (1) unit M is the same approximate age as estuarine terrace deposits bordering San Pablo Bay and Carquinez Strait, providing that the terrace deposits have been at diagenetic temperatures 1°-2°C warmer than unit M; and (2) the age of unit N is about four times greater than that of unit M, providing that both units have been at the same approximate diagenetic temperature.     

The Požarevac loess–paleosol sequence: a record of increased aridity in the south-eastern margin of the Carpathian Basin during the last 350 ka

The loess sequence preserved in the Požarevac brickyard in north-eastern Serbia comprises eight loess units separated by seven paleosols. Geochronological investigation using amino acid racemization and luminescence dating support stratigraphic correlations of loess units L3, S2LL1 and L1 at the Požarevac section with loess of glacial cycles E [Marine Isotope Stage (MIS) 10], D (MIS 9–8), C (MIS 7–6) and B (MIS 5–2) across central Europe. Correlation with the marine oxygen-isotope stratigraphy and associated paleoclimatic inferences are further supported by magnetic susceptibility, particle size and carbonate content measured in Požarevac sediments. Malacological investigations at the Požarevac section reveal the continuous presence of the Chondrula tridens and Helicopsis striata faunal assemblages throughout the last 350 ka. The loess malacological fauna, which is characterized by the complete absence of cold-resistant and cold-preferring species, suggests a stable, dry and relatively warm glacial and interglacial climate, compared with other central European loess localities. Together these data suggest that the south-eastern part of the Carpathian (Pannonian, Middle Danube) Basin was a refugium for warm-preferring and xerophilous land-snails during the generally unfavorable glacial climates of the late Middle and Late Pleistocene.     

A 3.5 ka record of paleoenvironments and human occupation at Angkor Borei,Mekong Delta,southern Cambodia

Microfossil and sedimentological data from a 3.1 m core extracted from a reservoir (baray) at the ancient Cambodian settlement of Angkor Borei in the Mekong Delta have provided a continuous record of sedimentation and paleoenvironments dating from about 2000 cal yr B.C. Palynological data indicate that for much of the cal. 1st and 2nd millennia B.C. mangroves dominated the regional vegetation, while extensively and regularly burnt grasslands dominated the local vegetation. Turbid, nutrient‐rich standing water characterized the core locality, perhaps suggesting a connection with rivers in the area. An abrupt change during the cal. 5th to 6th centuries A.D. involved a dramatic reduction in grasslands and the expansion of secondary forest or re‐growth taxa. These changes are synchronous with an abrupt decline in the concentration of microscopic charcoal particles in the sediments, and the colonization of the core locality by swamp forest plants. These changes are taken to indicate a shift in land‐use strategies or, possibly, a period of land abandonment. The age for the construction of the baray is interpreted to be in the 17th–19th centuries, but this dating remains speculative. Construction of the Angkor Borei baray exploited a preexisting body of standing water, so its construction was fundamentally different from the methods used at the Angkorian capital in northern Cambodia. © 2003 Wiley Periodicals, Inc.     

Amino acids in fossil corals: racemization (epimerization) reactions and their implications for diagenetic models and geochronological studies

Thirty-eight fossil coral samples of known age from late Pleistocene uplifted reef terraces have been analyzed for amino acid composition and for the degree of racemization of selected amino acids. Particular attention has been given to the epimerization (racemization about the α-carbon) of isoleucine. The $\text{D}\text{L}$ ratios observed in many of these samples do not conform to the concept of increasing racemization being associated with increasing fossil age. It is shown that sixteen of the samples demonstrate concordance between their known age and the age estimated from racemization data. Racemization ages are based upon a kinetic model derived from the kinetics observed in foraminifera in marine sediments and published estimates of the temperature dependence of the isoleucine epimerization reaction. Lack of concordance for the remaining samples is explained by either of two separate diagenetic phenomena: extensive leaching of free amino acids from the fossil, or contamination by ‘young’ amino acids. Ambiguities observed in many of the results may be due to the fact that coralline organic matter can have a complex history because of variations in its source and in its relation to the mineral phase. Neither of these factors has been observed to exert such a significant effect on racemization kinetics observed in fossil foraminifera or molluscs. Fossil corals are of only modest value as specimens for amino acid geochronological studies.     

Quaternary aminostratigraphy of eolianite on Lord Howe Island,Southwest Pacific Ocean

Amino acid racemization (AAR) dating of the eolianite on Lord Howe Island is used to correlate several disparate successions and provides a geochronological framework that ranges from Holocene to Middle Pleistocene time. The reliability of the AAR data is assessed by analysing multiple samples from individual lithostratigraphic units, checking the stratigraphic order of the D/L ratios and the consistency of the relative extents of racemization for a suite of seven amino acids. Three aminozones are defined on the basis of the extent of racemization of amino acids in land snails (Placostylus bivaricosus) and ‘whole-rock’ eolianite samples. Aminozone A includes Placostylus from modern soil horizons (e.g. mean D/L-leucine ratio of 0.03±0.01) and whole-rock samples from unconsolidated lagoonal and beach deposits (0.10±0.01–0.07±0.03). Aminozone B includes Placostylus (0.45±0.03) and whole-rock samples from beach (0.48±0.01) and dune (0.45±0.02–0.30±0.02) units of the Neds Beach Formation, deposited during OIS 5. The oldest, Aminozone C, comprises Placostylus recovered from paleosols (0.76±0.02) and whole-rock eolianite samples (0.62±0.00) from the Searles Point Formation, which indicate the formation was likely deposited over several Oxygen Isotope Stages (OIS), during and prior to OIS 7. These data support independent lithostratigraphic interpretations and are in broad agreement with U/Th ages of speleothems from the Searles Point Formation and corals from the Neds Beach Formation, and with several TL ages of dune units in both formations. The AAR data reveal that eolianite deposition extends over a significantly longer time interval than previously appreciated and indicate that the deposition of the large dune units is linked to periods of relatively high sea level.     

Alternative kinetic models for the interpretation of amino acid enantiomeric ratios in Pleistocene mollusks: Examples from California, Washington, and Florida

Alternative kinetic models for amino acid racemization in Pleistocene molluscan samples are compared by examination of results for samples from marine Pleistocene deposits in California, Washington, and Florida. Linear kinetic models previously have been applied to the Florida samples [Mitterer, R.M. (1975). Earth and Planetary Science Letters 28, 275–282.] because these kinetics were observed in laboratory pyrolysis experiments with the particular genus involved (Mercenaria). Nonlinear kinetic models, extrapolated from deep-sea foraminifera racemization kinetics, are applied to samples of Protothaca and Saxidomus from California and Washington and seem more consistent with their local chronologic and stratigraphic control. Average or effective diagenetic temperatures can be estimated by each of these models if reliably dated samples are available. Linear models applied to such samples from California and Florida suggest average diagenetic temperatures that are cooler (by as much as 10°C) than would be inferred from available paleoclimatic records. Nonlinear kinetic models yield estimates of average diagenetic temperatures that are more consistent with these records: full-glacial (i.e., approximately 18,000 yr BP) temperature reductions of between 2 and 6°C are inferred for coastal California and southern Florida. The nonlinear kinetic model is used to expand (by a factor of 2.5 to 3.0) the time scale proposed by Mitterer (1975) for five marine Pleistocene units of Florida.     

Aspartic acid racemization in Late Wisconsin Lake Ontario sediments

We have determined aspartic acid racemization and [¹⁴C] ages in sediment from a 17-m piston core recovered from Lake Ontario. This core represents a depositional record extending back into the Late Wisconsin. Total organic radiocarbon ages of the glaciolacustrine lower section of the core are older than the true depositional age. Hence we suggest that these ages do not represent time elapsed since deposition but rather may be up to several thousand years too old due to mixing with ¹⁴C-depleted organic matter and possibly also d-amino acids contained by the glacier and deposited as the glacier receded. Further studies are suggested that might test this hypothesis as well as establish the general applicability of racemization dating to clay sediments.     

Strontium-isotope stratigraphy as a constraint on the age of condensed levels: examples from the Jurassic of the Subbetic Zone (southern Spain)

Condensed levels are often characterized by reworked fossils that may lead to incorrect age assessments. Strontium‐isotope stratigraphy is an important chronostratigraphic tool that can be used to verify the biostratigraphic information from condensed beds. This paper describes a study of the ⁸⁷Sr/⁸⁶Sr isotope ratios of 56 belemnite samples collected from 28 stratigraphic sections of the boundary between the Upper Member of the Gavilán Formation and the Zegrí Formation (Pliensbachian, Subbetic Zone). The petrographic and geochemical data (δ¹⁸O, δ¹³C, concentrations of Fe, Mn and Mg, and the Sr/Mn ratio) suggest that the belemnites have preserved their original marine geochemical composition. After plotting the samples in diagrams of ⁸⁷Sr/⁸⁶Sr values against time according to their biostratigraphic age, four different groups (A, B, C and D) were obtained with respect to the reference curve. In groups A and B, the age deduced from the Sr‐isotope ratio is in total or partial agreement, respectively, with the biostratigraphic age; therefore the ⁸⁷Sr/⁸⁶Sr ratio is a good method for the dating, correlation and assessment of biostratigraphic results. In groups C and D, the SIS age and the biostratigraphic age do not coincide. A graphic procedure is presented as a suitable methodology to constrain the age of the samples showing an SIS age that differs from the relative age deduced (by biostratigraphy or stratigraphic correlation) for the bed they were collected in. These situations are interpreted as being the result of reworking of the belemnites (group C) or ammonites (group D) that are included in condensed levels. These condensed levels formed during the maximum flooding event that led to the drowning of the Gavilán carbonate platform. The methodology supplied in this paper represents a valuable tool in identifying reworking processes, improving correlation and constraining biochronostratigraphic results. The values of ⁸⁷Sr/⁸⁶Sr represent a new contribution to the data set of ⁸⁷Sr/⁸⁶Sr ratios for the Pliensbachian.     

Evidence from Ar/Ar ages of lunar impact glasses for an increase in the impact rate ∼800 Ma ago

Geochemical and ⁴⁰Ar/³⁹Ar data on nine impact glasses from the Apollo 14, 16, and 17 landing sites indicate at least seven distinct impact events with ages ∼800 Ma. Rock fragments analyzed by Barra et al. [Barra F., Swindle T. D., Korotev R. L., Jolliff B. L., Zeigler R. A., and Olsen E. (2006) ⁴⁰Ar-³⁹Ar dating of Apollo 12 regolith: implications for the age of Copernicus and the source of nonmare materials, Geochim. Cosmochim. Acta,70, 6016-6031] from the Apollo 12 landing site and some Apollo 12 spherules reported by Levine et al. [Levine J., Becker T. A., Muller R. A., Renne P. R. (2005) ⁴⁰Ar/³⁹Ar dating of Apollo 12 impact spherules, Geophys. Res. Let., 32, L15201, doi: 10.1029/2005GL022874.] show ∼800 Ma ages, close to the accepted age of the Copernicus event, 800 ± 15 Ma [Bogard D. D., Garrison D. H., Shih C. Y., and Nyquist L. E. (1994) ³⁹Ar-⁴⁰Ar dating of two lunar granites: The age of Copernicus, Geochim. Cosmochim. Acta, 58, 3093-3100]. These Apollo 12 samples are thought to have been affected by material from the Copernicus event since there is a Copernicus ray going through the Apollo 12 landing site. When all of these data are viewed collectively, including an Apollo 16 glass bomb [Borchardt R., Stöffler D., Spettel B., Palme H. and Wänke H. (1986) Composition, structure, and age of the Apollo 16 subregolith basement as deduced from the chemistry of post-Imbrium melt bombs. In Proceedings, 17th Lunar and Planetary Science Conference, pp. E43-E54], and in the context of diverse compositional range and sample location, there is a suggestion that there may have been a transient increase in the global lunar impact flux at ∼800 Ma. Therefore, the Copernicus impact event could have been one of many. If correct, there should be evidence for this increased impact flux around 800 Ma ago in the age statistics of terrestrial impact samples.     

Amino acid racemization dating of quaternary deposits of central and Southern Italy

Results are discussed of amino acid racemization dating of numerous samples of bones, teeth, tooth enamel and fossil shells. Racemization dating proved to be a very useful tool to date non-marine and marine deposits which are a few hundred thousand years old, as well as to correlate and chronologically classify even more ancient marine deposits. Aspartic acid racemization was used to date samples aged less than 80,000–100,000 yr; for more ancient samples, isoleucine epimerization, instead, was resorted to.In this work, dates were established for some important fragments of human bones and of large-mammalian fauna weighing a few grams, which would have been impossible to date with the ¹⁴C technique, requiring a large amount of bone material.The extent of isoleucine epimerization was measured in Glycymeris genera to estimate the age of numerous marine deposits of the Tyrrhenian coast of Centra-Southern Italy and of Sardinia. In the Tarquinia area (Latium) three marine terraces were identified, which were estimated to be approx. 120,000, 200,000 and 350,000 yr old. In Sardinia, five marine horizons were identified; the youngest deposit (Neotyrrhenian) proved to be present at Punta tramontana and Santa Reparata, while, at Riola and Stagno di Sassu, the most ancient marine deposits of Sardinia were found. In the Rome area, the Monte Mario Formation was estimated to be coeval with Monte delle Piche Formation. Finally, from the terraced coastal deposits of Capo Milaazzo (Sicily), Panarea (Eolian archipelago), Archi (Calabria) and Gallipoli (Apulia), a Tyrrhenian age range of 100,000–120,000 yr was obtained.     

Single and multigrain quartz‐luminescence dating of irrigation‐channel features in Santa Fe,New Mexico

Acequias (irrigation channels and ditches) were used by Spanish settlers, their descendants, and Native Americans in New Mexico. Several such features were recently excavated in Santa Fe, but material for numeric dating was difficult to find. Therefore, for this high‐energy‐deposition irrigation‐feature setting we applied optically stimulated luminescence (OSL) sediment dating methods to determine the timing of last filling of some of these acequias. We report multigrain single‐aliquot quartz (MGSAQ) OSL dating results and the first single‐grain quartz (SGQ) OSL dating results for irrigation features. One sample yielded an average age of 96 ± 13 yr, consistent with the maximum expected age of 127 yr (before 2007). An OSL age of 175 ± 15 yr for another sample delimits a sedimentation event since the first construction of that feature ca. 300 yr ago. A sample known to be younger than 400–450 yr but predating the mid‐19th century gave an SGQ age of 376 ±31 yr. These results indicate that: (1) Regional quartz in New Mexico is highly favorable to OSL dating; (2) in this setting, SGQ OSL dating is preferred to MGSAQ dating; and (3) for the last 500–600 yr, SGQ OSL dating in such settings is preferred to ¹⁴C dating because OSL dating lacks those ambiguities inherent in converting ¹⁴C ages to calendar years. © 2009 Wiley Periodicals, Inc.     

Amino acid age estimates, quaternary Atlantic Coastal Plain: Comparison with U-series dates, biostratigraphy, and paleomagnetic control

Amino acid enantiomeric (D/L) ratios in the mollusk Mercenaria are compared with recently published biostratigraphic and/or U-series solitary coral data from 22 Quaternary localities on the central and southern Atlantic Coastal Plain. In all cases, local relative aminostratigraphic sequences are consistent with relative ages inferred from U-series or biostratigraphic data, although occasionally more depositional events are recognized by aminostratigraphic than biostratigraphic methods. However, if the U-series data are used as age calibrations for the D/L values, latitudinal trends of “isochronous” D/L values are highly variable and conflict with trends expected from the present temperature gradient, which is smooth and nearly linear between 45° and 25° N. Age estimation can be performed independently of the U-series data using a kinetic model that relies on the assumption that Pleistocene temperature gradients have also been smooth functions of latitude, although significantly steeper than the present temperature gradient. Within the uncertainties of this assumption, kinetic model age estimates for localities in the coastal plain fall into the following groups: 70,000–130,000 yr, 200–250,000 yr, 300,000–400,000 yr, 500,000–600,000 yr, 700,000–800,000 yr, and > 1,000,000 yr. Major conflicts between these model age estimates are observed for localities near Charleston, South Carolina and in central Virginia. These conflicts could indicate that the basic temperature assumptions of aminostratigraphy are incorrect, and that apparent local aminostratigraphic sequences (clusters of different D/L values) could be due to factors other than age difference. Alternatively, some of the U-series dates may be only minimum ages for these localities.     

Early Farming in the Northern Boreal Zone: Reassessing the History of Land Use in Southeastern Finland through High‐Resolution Pollen Analysis

The early stages of agriculture in the Boreal forests of Northern Europe remain poorly understood. Although pottery appeared during the 6th millennium B.C., this has not been seen as an indication of a true Neolithic in the north. In later prehistory, vast parts of the region are thought to have remained a wilderness. In order to test these assumptions, a high‐resolution pollen analysis and an archaeological survey were carried out at Lake Huhdasjärvi, SE Finland. The results indicate signs of cultivation already by the early Neolithic, 5260–4260 B.C., and slash‐and‐burn cultivation concentrated on deciduous forests is recorded from ca. A.D. 600 onwards. By A.D. 930, an intensive form of swidden cultivation began in the coniferous forests, indicating a well‐established agricultural settlement. The discovery of Neolithic (late 6th millennium B.C.) buckwheat pollen suggests that the roots of agriculture in northernmost Europe may have to be searched for in China rather than the Near East.     

The Littorina transgression in the southwestern Baltic Sea: new insights based on proxy methods and radiocarbon dating of sediment cores

Rößler, D., Moros, M. & Lemke, W. 2010: The Littorina transgression in the southwestern Baltic Sea: new insights based on proxy methods and radiocarbon dating of sediment cores. Boreas, 10.1111/j.1502‐3885.2010.00180.x. ISSN 0300‐9483. The Littorina transgression is one of the most pronounced environmental events in the Holocene history of the Baltic Sea. It changed the hydrographic system from the freshwater Ancylus Lake into the brackish‐marine Littorina Sea. Here, 18 cores from two western Baltic basins, Mecklenburg Bay and the Arkona Basin, were analysed. We show that, besides biological indicators, sedimentary organic carbon, C/N ratio, bulk δ¹³C isotope values and carbonate content display clearly the transition from Ancylus Lake to the Littorina Sea. The first appearances of benthic foraminifers, marine molluscs and ostracods represent the onset of brackish‐marine conditions in the bottom waters. Central Arkona Basin sediments display more abrupt shifts in geochemical parameters and microfossil records at the transition from Ancylus Lake to the Littorina Sea than those from Mecklenburg Bay. Mixing of reworked Ancylus material with Littorina Sea stage material was stronger in Mecklenburg Bay, resulting in less pronounced proxy parameter changes and older bulk material dates. Radiocarbon dating of both calcareous material (benthic foraminifers, mollusc shells) and bulk fractions at the transgression horizon shows large age discrepancies. Based on calcareous fossil dates it appears that marine waters began to enter Mecklenburg Bay c. 8000 cal. a BP. In the Arkona Basin the first marine signals are recorded approximately 800 years later, c. 7200 cal. a BP. This indicates a transgression pathway via the Great Belt into Mecklenburg Bay and then into the Arkona Basin.     

桂林全新世石笋高分辨率δ13C记录及其古生态意义

桂林地区３个石笋分辨率达１０～１００ａ级的δ_１３Ｃ记录表明,全新世时期的古生态可以划分为森林植被由兴盛至退化(气候由暖湿至干凉)的３个旋回。早中期旋回人类活动影响较小,晚期旋回影响大,由于人类持续地对森林的破坏,草类植被得以生长,石漠化增强,石笋的δ_１３Ｃ值急速上升以后,很难再回复到全新世中期的水平。δ_１３Ｃ值大幅度跳跃式的下降,可以确定末次冰期旋回冰消期的终止点,其时限为１０ ７００ａＢ．Ｐ,与δ_１８Ｏ记录完全吻合,也可以与海洋氧同位素以及其它记录进行对比。     

Amino acid racemization in mammalian bones and teeth from La Chaise-de-Vouthon (Charente), France

A controlled test of the amino acid dating method as it is currently being applied to bones and teeth was attempted for the time range 100-250 ka, beyond that of ¹⁴C, at the archeological site of La Chaise-de-Vouthon (Charente, France). In Bourgeois-Delaunay, mammal fossils associated with Paleolithic artifacts were collected from layers dated at 101 ± 12 to 114 ± 7 ka by ²³⁰Th/²³⁰ dating of the over- and underlying stalagmitic floors. Racemization ratios for most amino acids were significantly lower than for comparably aged materials from other European sites. Regardless of the rate constants used, most amino acid dates were incorrect. Large associated errors spanned the late Pleistocene, making the dates useless for discriminating archaeological or geological events. Stratigraphic correlations using any tissue were also problematic. Relative interacid ratios varied with age, indicating that diagenetic alteration had affected racemization. Therefore, amino acid relative and absolute dating cannot be accurately applied to mid-Pleistocene bones or teeth.