细菌群落组成对微囊藻水华分解过程的响应

为探究细菌群落组成对微囊藻水华腐败分解过程的响应,在太湖梅梁湾沿岸进行为期11 d的原位围隔实验,模拟蓝藻水华聚集分解过程,并监测了此过程中水体环境因子和细菌群落组成.结果表明,水体理化因子和细菌群落组成在微囊藻水华分解的过程中发生了显著变化.冗余分析显示细菌的群落组成与水体氧化还原环境(溶解氧或氧化还原电位)、pH值、浮游植物生物量和营养盐浓度(总磷、硝态氮浓度)密切相关.研究还发现了某些与微囊藻水华分解密切相关的特殊细菌类群,其中隶属于黄杆菌科(拟杆菌门)的一个类群在微囊藻厌氧分解的阶段占据显著优势,其功能有待于进一步研究.     

Benthic microbial abundance and activities in an intensively trawled ecosystem (Thermaikos Gulf, Aegean Sea)

Abundance of benthic bacteria, heterotrophic nanoflagellates and ciliates, extracellular enzymatic activities, bacterial C production, C mineralisation and sediment community oxygen consumption rates were measured in the Thermaikos Gulf (Northeastern Mediterranean), before (September 2001), and during intense trawling activities (October 2001 and February 2002). The biochemical composition of sedimentary organic matter has revealed that bottom trawling had an effect on the trophic state of Thermaikos Gulf. Changes on the benthic microbial food web were also recorded, during the three sampling seasons. Even though trawling-induced sediment resuspension did not alter significantly the abundance of the microbial components, with the exception of the most impacted station, it determined changes regarding their relative importance. Thus, the ratios of bacterium to nanoflagellates and ciliate to nanoflagellates abundance increased in the trawled stations, causing a sudden increase in bacterial C production, in comparison to the non-trawled station. Four months later, the effects of trawling on the microbial food web were less evident, masked possibly by the drastic decrease in the water temperature. The results of the present work suggest that bottom trawling induces alteration of the sedimentological variables and can be considered as a factor affecting the function of the microbial food web in marine coastal ecosystems. These alterations cause faster mobilisation of organic C buried in the sediment and increase nutrient concentrations and availability in the system, thus inducing an effect that could lead to coastal eutrophication.     

Comparison of DNA-fingerprinting (T-RFLP) and high-throughput sequencing (HTS) to assess the diversity and composition of microbial communities in groundwater ecosystems

The majority of usable freshwater is stored as groundwater in the subsurface. Pristine groundwater ecosystems are characterised as oligotrophic environments which facilitate low energy yield, activity, growth, and reproduction for numerous and highly adapted organisms living in these environments. Degradation of groundwater quality and quantity are hence reflected in the structural changes of groundwater species communities. Despite an increasing awareness of this problem, current assessment methods for groundwater ecosystems are solely based on the analysis of abiotic parameters. However, this approach is insufficient to detect changes in microbial communities and their related metabolic functions. In recent years, the development of culture-independent molecular techniques to analyse microbes has vastly improved our knowledge concerning the diversity and composition of microbial communities in various environments. High-throughput sequencing (HTS) techniques enable the detection of single bacterial species in a sample and thus provide a high resolution of the composition and diversity of microbial communities in various environments. Furthermore, the taxonomic information obtained allows for the inference of metabolic functions of a given community. However, since the method is labour intensive and costly it is not necessarily the method of choice for analysing numerous samples. By comparison, DNA-fingerprinting is a less elaborate and inexpensive method that is able to detect changes in microbial communities, although identification of species present in a community is not possible, and therefore represents a valuable supplement to HTS. The present paper intends to render information about the applicability of this method as a monitoring tool against this background, by directly comparing results of DNA-fingerprinting with the results of HTS. Despite the fact that the analysis of bacterial communities using HTS captured significantly higher diversity estimates in our study, results of both methods were positively associated. And even though HTS produced more accurate and detailed results regarding composition and diversity of bacterial communities, patterns of community composition captured by DNA-fingerprinting were similar in comparison to HTS. We thus can suggest DNA-fingerprinting as a cost efficient alternative for community assessment and diversity estimation, specifically as a promising methodological approach in environmental assays.     

Cosmogenic21Ne and22Ne depth profiles in chondrites

The production rate profiles of²¹Ne and²²Ne as a function of depth in meteoroids due to spallation by solar flare cosmic rays (SCR) and galactic cosmic rays (GCR) are calculated and their dependence on size and composition of meteoroids has been evaluated. The GCR production rate at a given depth increases with size for radii<25cm and then decreases whereas the²²Ne²¹Ne ratio (NeR) generally decreases with size and depth. The calculated GCR production rates and NeR are consistent with the measurements in several Chondrites. A plot of track production rate vs. NeR shows that some chondrites have NeR values smaller than those expected for their sizes. Thes obeervation suggestsat least a two-stage irradiation for such meteorites; the meteoroid exposure as a small body in the interplanetary space must have been preceded by exposure under deep shielding, possibly in its parent body.     

Alteration of physico-chemical and microbial properties in freshwater substrates by burrowing invertebrates

The hyporheic interstitial provides habitat for many different organisms – from bacteria to burrowing invertebrates. Due to their burrowing and sediment reworking behaviour, these ecosystem engineers have the potential to affect hyporheic processes such as respiration and nutrient cycling. However, there is a lack of studies that characterize the interactions between bioturbators, physico-chemical habitat properties and microbial communities in freshwater substrates. In a standardized laboratory experiment, we investigated the effects of three functionally different bioturbators, duck mussels (Anodonta anatina, Linnaeus 1758), mayfly nymphs (Ephemera danica, Müller 1764) and tubificid worms (Tubifex tubifex, Müller 1774), on the physico-chemical conditions and bacterial communities in hyporheic substrates. We hypothesized that different invertebrates distinctly alter habitat conditions and thus microbial community composition, depending on the depth and the manner of burrowing. A. anatina and E. danica caused an increase in interstitial oxygen concentration, whereas strong declines in oxygen concentration and redox potential were detected in the T. tubifex treatment. These effects on physico-chemical habitat properties were even detectable in open water. Mussels and tubificid worms also significantly influenced the composition of bacterial communities in the hyporheic zone. A loss or replacement of bioturbators in stream ecosystems due to anthropogenic habitat alterations is expected to result in shifts in microbial community compositions, with effects on nutrient fluxes, pollutant degradation and benthic food webs. An understanding of the effects of functionally different native and invasive bioturbators is crucial to predict changes in stream ecosystem functioning.     

岱海水体及沉积物细菌多样性及群落组成特征

采用高通量测序技术,研究了内蒙古岱海流域入湖河流、湖水及沉积物细菌多样性及群落组成.结果显示,细菌多样性从高到低依次为：沉积物>河流>湖泊.聚类分析表明入湖河流、湖水和沉积物细菌群落可分为明显不同的3支,说明这3种生境中细菌群落结构有较大差异.物种注释结果表明,河流中优势细菌菌群为髌骨细菌(Patescibacteria)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria);湖水中优势细菌菌群为放线菌门(Actinobacteria);而沉积物中优势细菌菌群为变形菌门和绿弯菌门(Chloroflexi).典范对应分析及Monte Carlo检验表明,电导率和悬浮物含量对水体中(河流与湖泊)细菌群落影响显著,二者共解释了细菌群落变化的86.5%;而冗余分析及Monte Carlo检验表明,泥深、磁化率和总有机碳对沉积物中细菌群落影响显著,三者共解释了细菌群落变化的47.9%.近30年来,岱海地区气候变化和人类活动导致湖水咸化,沉积物碳氮指标显著增长.岱海水体及沉积物细菌多样性及群落组成的差异及其主要驱动因...     

Phytoplankton distribution and productivity in a highly turbid, tropical coastal system (Bach Dang Estuary, Vietnam)

Phytoplankton diversity, primary and bacterial production, nutrients and metallic contaminants were measured during the wet season (July) and dry season (March) in the Bach Dang Estuary, a sub-estuary of the Red River system, Northern Vietnam. Using canonical correspondence analysis we show that phytoplankton community structure is potentially influenced by both organometallic species (Hg and Sn) and inorganic metal (Hg) concentrations. During March, dissolved methylmercury and inorganic mercury were important factors for determining phytoplankton community composition at most of the stations. In contrast, during July, low salinity phytoplankton community composition was associated with particulate methylmercury concentrations, whereas phytoplankton community composition in the higher salinity stations was more related to dissolved inorganic mercury and dissolved mono and tributyltin concentrations. These results highlight the importance of taking into account factors other than light and nutrients, such as eco-toxic heavy metals, in understanding phytoplankton diversity and activity in estuarine ecosystems.     

Structure and seasonal dynamics of bacterial communities in three urban rivers in China

Although a large number of recent ecological studies have focused on freshwater bacterioplankton populations, knowledge of the primary factors influencing bacterial communities in urban rivers is limited. Bacterial community structure in three rivers located in Shanghai city was studied over a 1-year period using denaturing gradient gel electrophoresis (DGGE). Subsurface samples were collected every 2 months from two study sites in each river. Water was characterized when biological samples were collected by measuring temperature, pH, chloride ion, total dissolved solids (TDS), total N and total P. complex DGGE band patterns indicated high bacterial diversity in the rivers. Analysis of similarity (ANOSIM) showed that variation of the bacterial communities was higher between rivers than it was between samples from the same river. When evaluated using principal component analysis, samples collected during warmer months from any particular river tended to group together while cold-season samples generally clustered, suggesting significant seasonal impacts. Redundancy analysis was used to identify relationships between environmental factors and bacterial community composition in each individual river. Temperature, TDS, pH, TP and salt concentration were all identified as being related to bacterial community structure, with temperature being the most influential parameter in all three rivers. Our results indicated that bacterial community composition was different in the three different rivers. Even though the rivers were located in heavily disturbed urban ecosystems, temperature was the major driver of bacterial community composition, just as it is in natural systems.     

Effects of an oil spill on benthic community production and respiration on subtropical intertidal sandflats

This study determined effects of an oil spill on subtropical benthic community production and respiration by monitoring CO₂ fluxes in benthic chambers on intertidal sandflats during emersion before and after an accidental spill. The oil spill decreased sediment chlorophyll a concentrations, altered benthic macrofaunal community, and affected ecological functioning by suppressing or even stopping microalgal production, increasing bacterial respiration, and causing a shift from an autotrophic system to a heterotrophic system. Effects of the oil spill on the macrofauna were more severe than on benthic microalgae, and affected sedentary infauna more than motile epifauna. Despite the oil spill’s impact on the benthic community and carbon metabolism, the affected area appeared to return to normal in about 23 days. Our results suggest that the prompt response of benthic metabolism to exposure to petroleum hydrocarbons can serve as a useful indicator of the impact of an oil spill.     

全球变暖对淡水湖泊浮游植物影响研究进展

全球变暖对湖泊生态系统的影响已经成为近年来湖沼学领域的研究热点.本文首先列举了目前研究全球变暖对淡水湖泊浮游植物影响的常用方法:监测数据分析、时空转换、遥感信息提取、控制实验、模型预测和古湖沼学技术等.研究结果表明气候变暖导致的气温升高、湖泊热力分层提前破坏以及无冰期提前等因素可导致春季物候提前;在全球变暖大背景下浮游植物群落结构正朝着蓝藻占优的方向发展,但是不同地区以及不同物种对全球变暖的响应不一致.在营养盐充足的湖泊中,由于全球变暖延长了浮游植物生长季节等,从而能提高浮游植物初级生产力;但在贫营养湖泊中,浮游植物初级生产力与变暖趋势甚至可能呈负相关.由于生态系统往往是多因子的共同作用,这也使得全球变暖对浮游植物群落的影响效应复杂化,区分各因子的净影响份额是目前研究的一个难点;全球变暖引起的风场改变会促进浅水湖泊中营养盐从底泥的释放,同时也会增加水体中悬浮物的浓度而影响水下光场,因此开展气候变化对再悬浮及浮游植物群落结构的影响可能是将来研究的一个切入点.     

Regional millennial trend in the cosmic ray induced ionization of the troposphere

Long-term trends in the tropospheric cosmic ray induced ionization on the multi-millennial time scale are studied using the newly released paleomagnetic reconstruction models. Spatial and temporal variations of the tropospheric ionization has been computed using the CRAC:CRII model and applying the paleomagnetic CALS7k.2 reconstruction. It has been shown that long-term variations of the tropospheric ionization are not spatially homogeneous, and they are defined not only by solar (i.e., covariant with solar irradiance) changes but also by the geomagnetic field. The dominance of the two effects is geographically separated, which makes it possible to distinguish between direct and indirect solar–terrestrial climate effects. Possible climate applications are considered.     

Influence of slight differences in environmental conditions (light, hydrodynamics) on the structure and function of periphyton

Small streams are ecosystems mainly controlled by physical factors. Minor differences in these factors can affect periphyton, which are key functional communities in these ecosystems. Eight different environmental conditions combining two types of current, two flow velocities and two light intensities were produced and controlled in artificial channels. Their impact on young and mature periphyton was investigated during a 6-week exposure period. The two different levels of light intensity produced early effects on the algal community. In young periphyton, the lower level of light intensity enhanced the number of algal cells, and this community appeared to be significantly structured by light. As the periphyton matured, the effects of physical factors became more marked. At this later stage, both the bacterial and algal communities began to be affected. Both function (primary production) and structure began to respond to differences in light and in flow velocity. Small differences in low-level environmental factors, such as light and flow, had an effect both on the structure of periphyton and its functional capacities. Keeping in mind the close link between diversity and function in microbial communities, periphyton confronted to various environmental stresses (pollution, flooding) in the field may behave differently due to minor differences in physical factors.     

Effects of hypoxia on biofilms and subsequently larval settlement of benthic invertebrates

Biofilms on submerged surfaces are important in determining larval settlement of most marine benthic invertebrates. We investigated if exposure of biofilms to hypoxia would alter the larval settlement pattern and result in a shift in benthic invertebrate community structure in the field. Biofilms were first exposed to hypoxia or normoxia in laboratory microcosms for 7 days, and then deployed in the field for another 7 days to allow for larval settlement and recruitment to occur. Using terminal-restriction fragment length polymorphism of the 16S rRNA gene, this study showed that hypoxia altered the biofilm bacterial community composition, and the difference between the hypoxic and normoxic treatments increased with the time of exposure period. This study also demonstrated significantly different benthic invertebrate community structures as a result of biofilm exposure to hypoxia and that the hypoxic and normoxic treatments were dominated by Hydroides sp. and Folliculina sp., respectively.     

Cold fronts induce changes in phycoperiphyton structure in a shallow lake: Wind forces drive algae succession and nutrient availability

We evaluated wind as the main structuring force in driving phycoperiphyton structure, community composition and succession in a warm, polymictic shallow lake in southern Brazil. Mangueira Lake is continuously mixed due to its exposure to wind, and during cold fronts the wind changes from the dominant NE direction to a SSW direction. Our question was: could changes in wind forces induce phycoperiphyton succession and determine population structure? To answer this question, we studied the phycoperiphyton successional response to a change in wind forces under three different situations: on clean macrophyte leaves in an open site (Open) and in an enclosed site protected from the wind (Enclosure), and the Natural community growing on uncleaned macrophyte leaves in an open site. Cold fronts improved nutrient availability and changed the algal community. The phycoperiphyton natural community was dominated by Epithemia spp bound in gross masses of green filaments during cold fronts. However, the wind direction typically changes when cold fronts are over (NE-E) and wind blowing from the off-shore direction dislodged the attached algae biomass and pushed it toward to lake shore, inducing community changes. The macrophyte bank exhibited rapid colonization and acted as a refuge for phycoperiphyton, providing habitat heterogeneity, whereas the enclosure acted as a buffer against wind forces, delaying the succession derived from settlement of loosely adhered algae. Furthermore, in the enclosure, the succession only started after a strong disturbance (rain and wind > 10 m s⁻¹) in which lake water flooded the mesocosm inducing colonization. Phycoperiphyton showed resilience and recovered rapidly after the disturbance, when the rain supplied inocula and wind favored colonization with growth forms that take advantage of local conditions, depending of wind dynamics.     

Denaturing gradient gel electrophoresis shows that bacterial communities change with mid-ocean ballast water exchange

Ships carry ballast water for better stability and to control trim. However, the discharge of ballast water near ports is known to transport invasive species from one coastal area to another. The exchange of ballast water on the high seas is supposed to reduce such invasions of exotic species. In this study, we used denaturing gradient gel electrophoresis (DGGE) to analyze the composition of the bacterial community in ballast water before and after such a mid-ocean exchange, and we also measured total bacterial counts. Our findings confirmed that the ballast water was replaced by the mid-ocean exchange, as indicated by the marked change in the composition of the bacterial community. There was also a significant decrease in bacterial abundance after the mid-ocean exchange. Finally, our findings support the incubation hypothesis, because the composition of the bacterial communities changed over time within the same ballast water.     

Analysis of variations in the nitrogen oxide content in the polar atmosphere of the northern hemisphere during the solar proton flare of July 14, 2000, based on the UARS satellite data

Nitrogen oxides, produced as a result of ionizing proton impacts, have long lifetimes and substantially affect the ozone balance. Photochemical models give an increased production level of nitrogen oxides during solar proton flares. The usage of an increased NO production effectiveness value (molecule number per each ion pair) during increased ionization of the atmosphere in models can be among the causes. This value has been estimated based on satellite observational data. Data on the solar proton fluxes and the composition of the atmosphere have been used. The period of the proton event of July 14, 2000, has been considered. The NO production effectiveness, obtained when the observational data were analyzed, was much smaller than the value obtained previously theoretically. The causes of these differences should be studied additionally.     

Response of the bacterial community to in situ bioremediation of organic-rich sediments

A field trial experiment was carried out to assess the potential of bioremediation for mobilisation of carbon in organic-rich sediments. Both bioaugmentation (bio-fixed microorganisms) and biostimulation (oxygen release compounds--ORC) protocols have been tested and the response of the bacterial community has been described to assess the baseline for bioremediation potential. Multifactorial ANOVA revealed that bioaugmentation protocol had an effect in stimulate mobilisation processes and significantly enhanced extra-cellular enzymatic activity rates. In contrast biostimulation treatment did not have an effect on mobilisation rates but contributed to enhance bacterial efficiency through a maximization of the bacterial production:enzymatic activity ratio. Average calculation of net mobilised carbon showed that 23% increase of mobilised pool was accounted for bioaugmentation in summer. Although biostimulation accounted for a smaller increase in mobilised carbon (<10%), the use of ORC resulted in an increased mineralisation and net carbon loss via respiration. Based on our results, a conceptual model for application of bioremediation to face the problem of sediment eutrophication is discussed.     

青藏高原湖泊沉积物与水体细菌群落共发生网络和群落构建过程差异解析

研究高原湖泊沉积物和水体细菌群落结构的差异对于水生态治理与保护具有重要的科学意义。本文选取青藏高原中东部共19个湖泊为研究对象,采集沉积物和水体样本,使用高通量测序技术和统计分析,解析湖泊沉积物和水体细菌群落结构、空间分布特征、共发生网络模式、群落构建过程的差异及其原因。研究结果表明沉积物细菌群落alpha多样性指数(Chao1指数、Shannon多样性指数、Faith’s系统发育指数和Pielou均匀度指数)均显著高于水体细菌群落。沉积物和水体细菌的优势门均为Proteobacteria(相对丰度为45.33%和41.77%),而水体细菌群落结构具有较强的空间异质性。主坐标分析和相似性分析结果表明沉积物和水体中细菌群落结构差异极显著,海拔和纬度分别是青藏高原湖泊沉积物和水体细菌群落结构变化最显著的驱动因子。沉积物和水体细菌群落均具有明显的距离衰减模式,而沉积物细菌比水体细菌群落在空间上的周转率小。采样湖泊沉积物细菌群落共发生网络复杂度和稳定性高于水体细菌。沉积物和水体细菌群落的平均归一化随机率值分别为0.24和0.72,表明确定性过程在沉积物细菌群落构建的过程中起到了重要的作用,而水...