球等鞭金藻的异养培养

【目的】探索球等鞭金藻的异养培养条件。【方法】以球等鞭金藻3011为研究对象,对其进行避光异养培养,采用单因素实验,找出适合其异养生长的碳源、氮源及磷源。【结果与结论】葡萄糖、尿素和磷酸二氢钾分别为较适于球等鞭金藻异养培养的碳源、氮源和磷源;异养状态下球等鞭金藻生物量较高,在葡萄糖浓度40 g/L、尿素1.0 g/L、KH₂PO₄13.6 mg/L时微藻培养效果最佳,30℃下培养48 h微藻密度达109 mL^-1,培养96 h密度超过1010 mL^-1。     

福安肽-03的分离纯化及其一级结构解析

通过盐析、离子交换、凝胶过滤和疏水层析等分离纯化,同步进行抗血管生成和抗肿瘤生长检测,从赤魟(Dasyatis akajei)组织获得强抗血管生成成分福安肽-03(Fuantai-03,FAT-03)的纯品(纯度>95%);使用Edman降解法测定FAT-03 N-末端氨基酸组成和序列,RACE系统技术解析FAT-03的一级结构。结果表明:FAT-03明显抑制鸡胚绒毛尿囊膜血管生成和BALB/c裸小鼠Lewis肺癌的生长及肝转移,且其效果与剂量相关。FAT-03分子的一级结构即其氨基酸组成和序列已被确定。     

Comparative Analysis of Nutrient Composition of Caulerpa lentillifera from Different Regions

This study investigated the difference of nutrient composition in Caulerpa lentillifera collected from different regions.The nutrient compositions of C.lentillifera from China's Hainan and Shandong provinces were determined and compared with those from Semporna(Malaysia),Sabah(Malaysia),Petchburi(Thailand),and two other species of seaweeds(Malaysia).The results showed that the polysaccharide and protein contents of C.lentillifera from Hainan(44.82%±0.98%and 12.5%±0.70%),Shandong(43.22%±1.42%and 14.7%±0.72%),Petchburi(59.27%±2.31%and 12.49%±0.30%),and Sabah(44.02%±2.01%and 19.38%±1.48%)were higher than those of Eucheuma cottonii(26.49%±3.01%and 9.76%±1.33%)and Sargassum polycystum(33.49%±1.70%and 5.40%±0.07%),respectively.The polyunsaturated fatty acid contents in C.lentillifera from Hainan(29.98%)and Shandong(22.11%)were higher than that in Semporna(16.76%±0.27%),Sabah(9.49%),and S.polycystum(20.34%±0.43%),but lower than that in E.cottonii(51.55%±0.57%).In Caulerpa lentillifera of Hainan and Shandong provinces,the essential amino acid(EAA)/total amino acid(TAA)ratios were 35.22%and 38.78%,respectively;and the EAA/ETAA ratios were 54.36%and 63.35%,respectively.The EAA composition of C.lentillifera was close to the ideal model of Food and Agriculture Organization/World Health Organization.C.lentillifera was rich in mineral elements,including calcium((3315.85±127.55)mg(100 g)^-1 and(3728.35±92.38)mg(100 g)^-1),magnesium((6715.74±82.58)mg(100 g)^-1 and(8128.59±242.72)mg(100 g)^-1),and trace elements,including iron((510.65±5.47)mg(100 g)^-1 and(1972.97±183.35)mg(100 g)^-1),selenium((1.04±0.08)mg(100 g)^-1 and(0.83±0.15)mg(100 g)^-1),and zinc((33.90±0.13)mg(100 g)^-1 and(11.75±2.05)mg(100 g)^-1).These results showed that C.lentillifera was more nutritious than S.polycystum or E.cottonii in terms of polysaccharide,protein,and fatty acid contents.Furthermore,both C.lentillifera species collected from Hainan and Shandong provinces show more basic nutrients.Therefore,C.lentillifera has important development and application prospects.     

降雨变化对云县台垂直摆倾斜仪的影响分析

通过分析云县台2013～2016年降雨对垂直摆倾斜仪观测数据的影响发现,在春冬两季,主要受孤立的强降雨影响,当日降雨量达到15 mm以上时,便对倾斜量产生影响;在降雨较集中的夏秋季节,受连续降雨的影响,当日降雨量达到驱动降雨量后,降雨与倾斜量有很好的相关性,而雨季中期关系不明显。由于垂直摆倾斜仪观测值曲线与降雨累积值曲线存在很强的相关性,利用一元线性回归方法对降雨干扰进行剔除,有利于准确提取和识别前兆异常。     

图像分析计算水系分形维数的改进方法与应用

水系分形维数的计算与分析,有助于深入理解其与地貌发育、流域径流和侵蚀产沙等过程之间的关系。从分形维数的计算原理,提出了一种在MATLAB环境下,能够同时计算水系计盒维数和信息维数的方法,改进了现有通过图像分析计算分形维数的不足。采用已知分形维数的规则分形集进行验证,结果表明:该方法单独对图像载体上的目标图形(关注区域)进行处理,不受其与整幅图像的比例的限制能够自动确定最佳的对目标图形进行网格分割覆盖的递减等分条件,计算精度高利用元胞数组实现了网格的自动分割,程序紧凑,可读性增强。最后以泾河、北洛河水系为例,说明了该方法的应用过程。本研究为水系分形维数的科学计算提供了新的思路。