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1.
A laboratory study was conducted to evaluate the response of soil enzyme activities (namely dehydrogenase, phosphatase and urease) to different levels of trace element pollution in soil representative area. The improved ecological dose model and random-amplified polymorphic DNA (RAPD) were used to assess soil health. The 50% ecological dose (ED50) values modified by toxicant coefficient were calculated from the best-fit model, and determination values from the regression analysis for the three enzyme activities were studied after the incubation periods. The results showed that the elevated heavy metal concentration negatively affects the total population size of bacteria and actinomycetes and enzymatic activity; dehydrogenase (ED50 = 777) was the most sensitive soil enzyme, whereas urease activity (ED50 = 2,857) showed the lowest inhibition; combined pollution or elevated toxicant level would increase disappearing RAPD bands, and the number of denoting polymorphic bands was greater in combined polluted soils. All three mathematical modified models satisfactorily described the inhibition of soil enzyme activities caused by Cd and Pb, by giving the best fit.  相似文献   

2.
对甘南玛曲沼泽湿地6个样地的微生物量与土壤酶活性及其土壤理化性质之间的关系进行了研究.结果表明:纤维素酶活性在冬季达到高峰,其余5种土壤酶活性分别在春季和秋季达到高峰;硝化细菌数量与过氧化氢酶、纤维素酶、脲酶、碱性磷酸酶有较显著的负相关关系;微生物量与酶活性之间存在极显著相关关系,其中微生物量因子中的放线菌、氨化细菌、硝化细菌和土壤酶活性因子中的酸性磷酸酶、碱性磷酸酶、中性磷酸酶是引起相关性的主要因素;酸性磷酸酶活性与土壤含水率、pH值、硝态氮含量、氨态氮含量都有密切的关系,过氧化氢酶活性可以通过有机质的含量来反映,脲酶活性可以通过土壤的含水率来反映,碱性磷酸酶活性可以通过电导率来反映.  相似文献   

3.
为了研究马衔山多年冻土区和非多年冻土区土壤微生物碳氮、土壤酶活性的差异,选取多年冻土区、季节冻土区和交界区为对象,分析了0~30 cm土层微生物碳氮和转化酶、脲酶、中性磷酸酶、淀粉酶、过氧化氢酶、多酚氧化酶酶活性不同季节的变化特征。结果表明:全氮、总有机碳、微生物量碳氮与多数土壤酶之间呈显著相关关系。在不同区域,土壤微生物碳氮均在0~10 cm含量最高,10~20 cm次之,20~30 cm最低。土壤微生物碳氮在生长季表现为含量逐渐增加,但是多年冻土区与季节冻土区差异不大。土壤酶活性在深度方面表现与微生物碳氮含量变化一致。土壤酶并无的季节变化规律。在多年冻土区,转化酶、多酚氧化酶和磷酸酶活性明显高于非多年冻土区。本研究表明,尽管多年冻土区的植被和土壤总有机碳明显高于非多年冻土区,其土壤微生物碳氮含量相当,且一些土壤酶活性也相当。说明非多年冻土区土壤的生物地球化学相对强度较大。因此,多年冻土退化后可能会导致生态系统的退化。  相似文献   

4.
This study evaluated the efficiency of naturally occurring lime-based waste materials (oyster shells, eggshells, and mussel shells) on immobilization of selected heavy metals (Cd and Pb) and a metalloid (As) in a contaminated agricultural soil. A 30-day incubation experiment was performed using soil mixture with natural liming materials or calcite (CaCO3) at 0, 1, 3, 5, and 10 wt %. Soil biochemical properties including pH, electrical conductivity (EC), exchangeable cations, organic matter (OM), total nitrogen (TN), microbial populations, and enzyme activities were determined to ensure the changes in soil quality during incubation. The results showed that the application of natural liming materials led to an increase in soil pH similar to that of CaCO3. Soil concentrations of Cd, Pb, and As extracted with 0.1 or 1 M HCl, and diethylene triamine pentacetic acid (DTPA) were decreased significantly after adding liming materials, accompanied by increased microbial population and enzyme activities of dehydrogenase, phosphatase, β-glucosidase, and arylsulfatase. Additionally, eggshells and mussel shells induced significant increases in OM and TN in the soil. Application of natural liming materials offers a cost-effective way to immobilize heavy metals and metalloids in soils.  相似文献   

5.
Studies on seasonal changes of heavy metal concentration in soils provide vital information for best management options at all times. The study investigated temporal variation in concentration of heavy metals in three towns having automobile service centres in Imo State. The study site is characterized by two major seasons in a year. Heavy metals were found in both arable and automobile soils, but more concentrations were recorded on the latter. Mean values of Cd, Cr, Ni, Hg and Pb were 6.2 mg/kg, 4.7 mg/kg 6.5 mg/kg, 0.02 mg/kg and 71.9 mg/kg respectively in the dry season while 2.9 mg/kg Cd, 2.2 mg/kg Cr, 1.9 mg/kg Ni, 0.01 mg/kg Hg and 51.9 mg/kg Pb were recorded during the rainy season of the experimental period. Higher values of heavy metal concentration were found in automobile soils as follows: 18.1 mg/kg Cd, 12.0mg/kgCr, 16.3 mg/kg Ni, 4.8 mg/kg Hg and312.8 mg/kg Pb in rainy season, and 15.1 mg/kg Cd, 8.1 mg/kg Cr, 11.9 mg/kg Ni 2.7 mg/kg Hg and 267.9 mg/kg Pb. However, Cd showed highest variability in arable soils during the dry season (CV=79%) while Hg varied widely in automobile soils in the rainy season (CV=54%).  相似文献   

6.
Reclamation of saline soil plays an important role in supporting high population growth in China. To evaluate the effects of reclamation and sustainability of salt-affected land production, soil chemical properties, enzyme activities, microbial community structure and function in sites reclaimed in 1976, 1984 and 1996 were characterized. The 2009 site was left to succession fallow and chosen as a control site. Results showed that electrical conductivity (EC) and pH decreased rapidly after the soil forming process started. An accumulation of soil organic C as a result of farming was observed. In all sites, inorganic N and available P were increased within 33 years after reclamation and commencement of agriculture. As a result of reclamation, soil enzyme activity (β-glucosidase, phosphatase, urease and arylsulfatase) was increased. There were no significant differences in soil enzyme activity between the sites reclaimed in 1976 and 1984, with the exception of phosphatase. Carbon source utilization patterns were less diverse in control soil than in treatment sites. The activities of reclamation resulted in synthesis of new phospholipid fatty acids (14:0, 17:1 c9, 16:0 2OH, 17:0 10Me, i17:0, 20:4 ω6c). Principal component analyses showed that the sites reclaimed in 1976 and 1984 clustered together and were distinct from 1996. Taken together, reclamation showed significantly increased soil quality and microbial activity. EC was the main limiting soil quality characteristic, which showed a comparative steady state after a reclamation time of 33 years. Differences in soil enzyme activity and microbial community function after long-term reclamation have potential to be reflected in soil functional integrity and ecosystem service.  相似文献   

7.
Theeco environmentalpollutioncausedbyminingdevelopmentisaworldwideproblem ,whichhasarousedever increasingattentionofscientists.Inadditiontovegetationdestructionanderosionofcultivatedlanddirectlycausedbyminingdevelopment,scientistshaveplacedmorefocusontheenvironmentalproblemsinducedbythereleaseofharmfulsubstances ( particularlyheavymetals) .Especiallyundersurfaceconditions,thepiled upminewastes (minetailings)undergoweatheringundertheactionofaseriesofgeochemicalfactorssuchasthemineralogyofmineta…  相似文献   

8.
祁乐  高明  杨来淑  王丹  邓炜 《中国岩溶》2015,34(1):86-94
文章以重庆市丰都县三坝乡土地整理项目为例,分别对土地整理前(2012年12月)52个采集点和土地整理后(2013年3月)28个采集点,采用野外采样和室内分析相结合的方法,研究了土地整理对岩溶区土壤微生物群落结构、土壤微生物生物量碳(SMBC)、氮(SMBN)及酶活性(过氧化氢酶、脲酶、蔗糖酶)的影响,结果表明:(1)土地整理对土壤微生物影响显著,土地整理后比整理前土壤中的放线菌数量降低,真菌数量增加。(2)在0~20 cm土层,整理前后SMBC和SMBN含量变化显著,SMBC含量整理后比整理前降低了20.33%,SMBN含量整理后比整理前减少了47.84%;土地整理改变了整理前SMBC、SMBN含量随土层深度变化递减的规律,使SMBC、SMBN含量分别在亚表层(20~40 cm)出现最小值68.34 mg/kg和最大值33.58 mg/kg。(3)整理前后土壤脲酶和过氧化氢酶均在0~20 cm和20~40 cm同一土层差异显著,蔗糖酶活性在各土层差异均显著。土地整理使土壤脲酶和过氧化氢酶活性降低,蔗糖酶活性提高。(4)有机质对表层SMBC、SMBN含量和脲酶活性呈显著正相关,pH值与土壤酶活性呈显著正相关。   相似文献   

9.
通过对河北省宣化原煤及燃煤污染区表土和近地降尘的野外调查和样品分析,查明了表土及降尘中Hg、Cd、V、Zn、Mo、U、Mn、Cr等13种重金属元素及16种多环芳烃(PAHs)的地球化学特征。结果表明,原煤区土壤以Hg、Cd污染为主,污染区在煤带两侧约100 m范围内,燃煤区土壤以Hg、Cd、V污染为主,污染范围在燃煤企业下风口超过2 km。原煤区表土中Hg、Cd、Zn、Mo、U及PAHs与燃煤区表土中Hg、V、Mo、U、Mn、Cr及PAHs主要通过大气沉降带入。区内土壤中脲酶、碱性磷酸酶及β-葡萄糖苷酶活性均受到不同程度抑制,其中以β-葡萄糖苷酶最为明显,且燃煤区较原煤区抑制更为显著,反映了煤污染对土壤碳循环过程影响较大。  相似文献   

10.
Comparative proteome analysis of butachlor-degrading bacteria   总被引:1,自引:0,他引:1  
A Pseudomonas putida strain, named ER1, was isolated from an agricultural soil and found to actively degrade the herbicide butachlor. The enzyme extracted from ER1 could degrade butachlor. Furthermore, incubation of ER1 in a medium containing 50 mg/kg of butachlor after 3 days resulted in the high butachlor-degrading enzyme activity of ER1. Response of ER1 to butachlor might be related to changes in protein composition at both quantitative and qualitative levels. Total proteins were extracted from control strain (incubated in the medium without butachlor) and the treated strain (incubated in the medium with butachlor). The proteins were separated by two-dimensional gel electrophoresis. Of the total number of ER1 protein, 11 spots were significantly changed under butachlor stress. Analysis by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry coupled with database searching allowed the function of some proteins which were similar to the hydrolases activity or oxidoreductase activity.  相似文献   

11.
A crude contaminated soil, arising from an oil production zone in Tabasco, Mexico was studied. A sample of about 40 kg was dried and screened through meshes 10–100. Total petroleum hydrocarbons and 6 metals (Cd, Cu, Cr, Ni, V and Zn) were determined to the different portions. For soil which passed mesh 10, six non-ionic, three anionic and one zwitterionic surfactant solutions (0.5%) were employed to wash the soil. Additional tests using surfactant salt mixtures and surfactants mixtures were carried out. Once the best soil washing conditions were identified, these experimental conditions were applied for washing the rest of the soil portions obtained (meshes 4, 6, 20, 40, 60, 80, 100). Total petroleum hydrocarbons values were in the range of 51, 550 to 192, 130 mg/kg. Cd was not found in any of the soils portions, and the rest of the metals were found at different concentrations, for every soil mesh. Treatability tests applied to the soils indicated that it is possible to get removals between 9.1 to 20.5%. For the case of a sodium dodecyl sulphate 1% solution, total petroleum hydrocarbons removal was as high as 35.4%. Combinations of sodium docecyl sulphate and salts, gave removal rates up to 49.5%. Total petroleum hydrocarbons concentrations for the whole soil were about 150,600 mg/kg. The higher the particle size, the lower the washing removal rate. The combined effect of particle size and total petroleum hydrocarbons concentration, determines the total petroleum hydrocarbons removal efficiencies. These facts are very important for designing an appropriate soil washing remediation process.  相似文献   

12.
以海泡石、膨润土和生石灰为修复材料,对湖南株洲地区重金属元素Cd、Pb污染农田土壤进行了室内修复实验。盆栽小白菜的正交实验结果表明,对于Cd含量为1.23 mg/kg、Pb含量为136.7 mg/kg的土壤,当每10 kg土壤添加膨润土60 g、海泡石20 g、石灰6 g时,种植的小白菜中Cd含量为0.154 mg/kg,Pb含量为0.141mg/kg。与未修复土壤的实验结果对比,小白菜中Cd含量降低61%,Pb含量降低46%。  相似文献   

13.
甘肃嘉峪关市表层土壤重金属空间分布与评价   总被引:1,自引:0,他引:1  
为研究嘉峪关市重金属分布对环境的影响,分析了嘉峪关市表层土壤重金属分布和含量变化,并评价其富集程度,判断其来源和影响因素。采用电感耦合等离子体质谱仪(ICP-MS)测量嘉峪关市134个表层土壤样品中六种重金属元素(Cr、Cd、Cu、Pb、Ni、Zn)的含量,其平均含量分别为281.6 mg/kg、0.35 mg/kg、60.68 mg/kg、51.39 mg/kg、108.65 mg/kg、161.0 mg/kg。在土壤重金属含量空间分布的基础上,用内梅罗指数法和地累积指数法对研究区土壤重金属富集程度进行了评价,六种元素地累积指数排序依次为: Cr > Cd > Pb > Cu > Zn > Ni,各功能区重金属元素整体富集程度依次为工业区 > 戈壁 > 生活区 > 农业区。戈壁采样点重金属元素含量(除Ni外)高于农业区,除工业因素外,地表植被的缺失加剧了戈壁地区重金属元素的富集。结合主成分分析,重金属元素空间展布,及内梅罗指数评价和地累积指数评价,分析了各元素可能的来源,认为Cr、Zn主要来自以钢铁生产加工为主的工业源,Cd、Cu、Pb来自于交通源,Ni可能与钢铁生产或当地背景值有关。通过分析嘉峪关市土壤重金属分布情况,以期为改善当地土壤质量提供科学依据,为我国西北地区土壤重金属的研究提供参考。  相似文献   

14.
从长江经济带某在役石化场地采集了3个点位不同深度的土壤样品,测定了石油烃等污染物的含量和微生物的群落结构,以揭示污染物分布规律和微生物特征。结果显示,3个点位污染程度不同,但污染物含量均随深度先升高后降低,最高含量均出现在埋深4.0 m左右,即地下水位附近。土壤中C6-C9高达5 302 mg/kg,C10-C40高达625.6 mg/kg,芳烃高达455.2 mg/kg,卤代烃高达41.54 mg/kg。土壤微生物的丰富度和多样性随深度增加而降低,同一深度土壤微生物的丰富度和多样性随污染物含量增大而降低。门水平上Actinobacteria、Firmicutes和Spirochaetes的相对丰度和石油烃含量显著正相关(p<0.05),属水平上Streptomyces、SCADC1-2-3、DesulfitobacteriumCryptanaerobacter的相对丰度和石油烃含量显著正相关(p<0.05)。  相似文献   

15.
The investigation of the impact of different forms of nitrogen fertilizer (NO3-N and NH4-N) on microbial parameters, enzyme activities and phytotoxicity in a petroleum-contaminated soil was evaluated by an incubation study. The tested enzymes, microbial activity and seed germination index showed different patterns in response to both petroleum and nitrogen fertilizer addition and time of incubation. The results apparently showed that the contamination of soil with petroleum has a negative effect on soil ecosystem. Nitrogen fertilizer could improve inhibition of petroleum hydrocarbons in soil. Nevertheless, nitrogen fertilizer had no significant effect on urease activity in the petroleum-contaminated soil. As compared to NO3-N, the addition of NH4-N to the soil resulted in a greater impact on soil performance as attested by the recovery of the soil germination capability and higher values of the respiration. The application of nitrogen fertilizer may be suggested as a good strategy for restoring soils in regions affected by the same problem.  相似文献   

16.
Urban soil nitrogen and phosphorus have significant implications for the soil and water quality in urban areas. The concentrations of total nitrogen (TN) and total phosphorus (TP) of soil samples collected from six types of land use, which included residential area (RA), business area (BA), classical garden (CG), culture and education area (CEA), public green space (PGS) and roadside area (RSA) of Beijing urban area, were investigated. Results showed that the geometric mean of TP (857 mg/kg) in urban soils was slightly higher than that (745 mg/kg) in rural soils of Beijing. The concentration of soil TP was higher in the center of the city, and showed an increasing trend with the age of the urban area. The TP concentrations in the six types of land use followed the sequence of CG > BA > RSA > RA > CEA > PGS, which were affected by the use and disposal of phosphorus-containing materials in each type of land use. However, the geometric mean of TN (753.8 mg/kg) in urban soils was much lower than that (1,933.3 mg/kg) in rural soils. TN level in urban soils of Beijing had no correlation with the city’s urbanization history, and was influenced by the coverage of natural vegetation and human activities in each type of land use. This study suggested that the city’s urbanization history and land use were the main factors affecting the distribution of nitrogen and phosphorus in urban soils.  相似文献   

17.
Effects of heavy metals on some soil biological parameters   总被引:11,自引:0,他引:11  
The soils most polluted by heavy metals are probably alluvial soils. One of them is the alluvium of the Litavka River. The heavy metal content was much higher in some cases than the threshold values, allowed by the Czech standards. The effect of soil contamination on enzyme activity is presented. Generally, the values of enzymatic activities were highest in the soil above the source of contamination and they decrease as they approach the source of contamination. All measured parameters were affected by heavy metal concentrations. Our results demonstrate that several parameters of microbial activity could be used as good indicators of increasing concentrations of Cd, Pb and Zn in soil.  相似文献   

18.
广东省普宁市土壤硒的分布特征及影响因素研究   总被引:2,自引:0,他引:2  
开展了广东省普宁市区域土壤硒调查研究,采集了413个表层土壤样品(0~20 cm)和103个深层土壤样品(> 150 cm),测定了土壤全硒含量,据此研究土壤硒分布特征及其影响因素。结果表明,普宁市土壤全硒含量变化于0. 16~2. 01 mg/kg,平均值为0. 63 mg/kg,总体上处于中硒及高硒水平,不存在缺硒和硒过剩土壤。砂页岩风化形成的赤红壤全硒含量较高,平均值达0. 86 mg/kg,以侏罗系页岩母质发育的土壤全硒含量最高,平均值达0. 89 mg/kg;三角洲第四系沉积物发育形成的水稻土全硒含量最低,平均值为0. 41 mg/kg。回归分析表明,土壤全硒含量与铁铝含量、有机碳含量具有极显著正相关,与p H呈极显著负相关。影响普宁市土壤硒含量的主要因素是成土母质,土壤p H、有机碳和铁铝含量及土地利用方式对土壤全硒含量分布与富集也有一定的影响。  相似文献   

19.
岩溶石山地区土壤退化与恢复的成因和机理一直是近年来土壤学和生态学研究的热点。以贵州西南部贞丰—关岭岩溶石山区为对象,选择了区内退化地、农用地、恢复地等生态样块以及与未退化地为对照,采集了其中的表层和亚表层土壤,分别进行了土壤的养分库量、微生物活性与功能和土壤酶活性等化学分析。研究表明土壤养分库是土壤中微生物活性基础,微生物量碳随土壤养分库容的退化或恢复而相应变化,退化岩溶地植被恢复3~6年后,主要养分总库容得到明显恢复(恢复程度55%~65%),因而带动了微生物量碳的恢复(平均恢复程度60%以上),但养分的活性(有效磷)、微生物的功能(呼吸熵及土壤脲酶和磷酸酶活性)并没有得到相应程度的恢复(平均恢复程度仅在25%~40%)。这些结果提示,限制性养分活性和微生物功能的恢复是植被恢复下生态系统健康水平的重要指示。因此,在分析岩溶土壤和生态系统退化过程的本质以及评价生态恢复的效应时,不仅应将微生物量碳和总养分库指标作为岩溶土壤退化恢复的指标,更应将微生物区系的质量和功能指标纳入关键评价内容。   相似文献   

20.
氨基泡塑的合成及其应用于富集地质样品中的痕量金   总被引:3,自引:2,他引:1  
采用泡塑(PUF)富集,AAS或ICP-OES测定地质样品中痕量金是常用的分析方法。与活性炭相比,PUF的选择性好,但吸附容量偏低,可将泡塑负载不同的萃取剂或修饰不同的官能团提高吸附容量。本文将聚醚型泡塑经盐酸水解制备成氨基泡塑(PUF-NH_2)。红外光谱和扫描电镜表征显示,PUF-NH_2峰形发生了明显红移(3376.5 cm-1),其中的氨基数量显著增加,另外PUF-NH_2的高分子出现明显断裂,发生水解后裸露出的氨基具有还原性,在吸附金的过程中易与金离子在PUF-NH_2表面发生氧化还原反应,形成金纳米颗粒。改性后的PUF-NH_2吸附容量达到96 mg/g,与PUF相比提高了8倍。将PUF-NH_2应用于富集地质样品中的金,经炭化灼烧、50%王水提取后用ICP-OES测定,金的加标回收率在95.0%~105.0%之间,检出限为0.15μg/g。实验证明用PUF-NH_2处理样品提高了富集倍数和分析灵敏度,有利于低品位矿石的分析。  相似文献   

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