THE DENSITIES OF CHANGJIANG ESTUARINE WATERS

The International Eq一lation of State of Seawater,1980 and the PraeticalSalinity Scale,1978 have been adoPted by theUNESCO江CES沼COR八APSOJoint Panel ono‘eanogral,hie Tables and Standards(JPOTS),and endorsed bythese organizati6ns(Miller‘〕and Poisson,1981;Uneseo,1981).Th已new equa-tion and the Praetieal Salinity Seale are to be used for all values Published fromJan .1,1982 .The new equation 15 aeeurate for use in all oeeanie surfaee waters,but eannot be aeeurately aPPlied to…     

Effects of temperature and irradiance on early development of Chondrus ocellatus Holm (Gigartinaceae, Rhodophyta)

Chondrus is a type of commercially produced red seaweed that widely used for food and carrageen extraction. Although the natural life history of the alga had been well understood, the factors influencing development of the tetraspore and carpospore remain poorly understood. In the perspective of seedling resources, the regulation of early development is crucial for the seedling nursing; therefore, it is necessary to understand the physiological influences during its early development. In this study, we studied the effects of temperature and irradiance on the early development of Chondrus ocellatus Holm under laboratory conditions. The released tetraspores and carpospores were cultivated at different temperatures (10–28°C) and irradiances (10, 60 μmol photons m⁻²s⁻¹) with a photoperiod of 12L:12D. The results indicate that both tetraspores and carpospores are tolerant to temperatures of 10–25°C, and have the highest relative growth rate at 20°C. Irradiance variances influenced the growth of the discoid crusts, and the influence was more significant with increasing temperature; 60 μmol photons m⁻²s⁻¹ was more suitable than 10 μmol photons m⁻²s⁻¹. The optimum temperature and irradiance for the development of seedlings was 20°C and 60 μmol photons m⁻²s⁻¹, respectively.     

Sea flux of trace metals in Saanich Inlet

The sea fluxes of trace metals, POC, and settled material were studied in anoxic seawater, Saanich Inlet, B. C., Canada with sediment traps. This paper discusses the change of mass fluxes of sediment, trace metals and POC for various seasons and depths. The annual mean of settled material is 1.56 g.m⁻².a⁻¹, 84.6 mg. m⁻².a⁻¹ for POC, 60.0 mg. m⁻².a⁻¹ for Cu, 16.5 mg.m⁻².a⁻¹ for Pb, 189 mg.m⁻².a⁻¹ for Zn, 2.20 mg.m⁻².a⁻¹ for Cd, 699 mg.m⁻².a⁻¹ for Fe, 38.8 mg.m⁻².a⁻¹ for Co, and 84.6 mg.m⁻².a⁻¹ for Ni. The relations between the average fluxes of trace metals and POC, the fluxes of trace metals and settled matter, and the sea fluxes of trace metals and Fe are in linear progression. The resident times of elements as calculated from sea flux, are 1.1 a. for Cu, 0.014 a. for Pb, 0.50 a. for Zn, 3.8 a. for Cd, 0.16 a. for Fe, 0.39 a. for Co, and 1.14 a. for Ni. The order of resident times is as follows: Pb−Fe−Co−Zn−Cu−Ni−Cd. The metal resident times in Saanich Inlet are shorter than the values estimated for the open ocean. It illustrates that the biochemical processes in shallow Saanich Inlet are faster than those in the open ocean, and that debries of plankton and fecal pellets of zooplankton play an important role in vertical transport of organic carbons. Contribution No. 1650 from the Institute of Oceanology, Academia Sinica.     

<Emphasis Type="Italic">p</Emphasis>CO<Subscript>2</Subscript> and carbon fluxes across sea-air interface in the Changjiang Estuary and Hangzhou Bay

Partial pressure of CO2 （pCO2） was investigated in the Changjiang （Yangtze River） Estuary, Hangzhou Bay and their adjacent areas during a cruise in August 2004, China. The data show that pCO2 in surface waters of the studied area was higher than that in the atmosphere with only exception of a patch east of Zhoushan Archipelago. The pCO2 varied from 168 to 2 264 μatm, which fell in the low range compared with those of other estuaries in the world. The calculated sea-air CO2 fluxes decreased offshore and varied from -10.0 to 88.1 mmol m^-2 d^-1 in average of 24.4 ± 16.5 mmol m^-2 d^-1. Although the area studied was estimated only 2 × 10^4 km^2, it emitted （5.9 ± 4.0） × 10^3 tons of carbon to the atmosphere every day. The estuaries and their plumes must be further studied for better understanding the role of coastal seas playing in the global oceanic carbon cycle.     

A STUDY ON THE RELATIONSHIPS OF THE NUTRIENTS NEAR THE CHANGJIANG RIVER ESTUARY WITH THE FLOW OF THE CHANGJIANG RIVER WATER

Investigations from August, 1985 to July , 1986 showed that the high concentration area of PO4-P , SiO3-Si and NO3-N gradually reduced with the reduction of the area of the Changjiang River diluted water from summer, autumn to winter , and that the seasonal distributions and variations of the nutrients concentrations were mainly controlled by the river flow and were also related to the growth and decline of phytoplankton . The conservation of SiO3-Si and NO3-N in the estuary in the flood season was poorer than that in the dry season .. The behaviour of PO4-P in the estuary shows that aside from -biological removal, buffering of PCU-P is possible in the estuary . The highest monthly average concentrations and annual average concentrations in the river mouth were respectively 0.88 and 0.57 umol/L for PO4-P,191.5 and 96.2 umol/L for SiO3-Si, and 81.6 and 58.6 umol/L for NOs-N . The Changjiang's annual transports of PO4-P , SiO3-Si and NO3-N to the sea were about 1.4×104tons , 204.4×104 tons and 63.6×104     

Optimization of conditions for tetraspore release and assessment of photosynthetic activities for different generation branches of Gracilaria lemaneiformis Bory

Gracilaria lemaneiformis Bory is an economically important alga that is primarily used for agar production. Although tetraspores are ideal seeds for the cultivation of G. lemaneiformis, the most popular culture method is currently based on vegetative fragments, which is labor-intensive and time-consuming. In this study, we optimized the conditions for tetraspore release and evaluated the photosynthetic activities of different colonies formed from the branches of G. lemaneiformis using a PAM (pulse-amplitude-modulated) measuring system. The results showed that variations in temperature and salinityhad significant effects on tetraspore yield. However, variations in the photon flux density (from 15 μmol m⁻² s⁻¹ to 480 μmol m⁻² s⁻¹) had no apparent effect on tetraspore yield. Moreover, the PAM-parameters Y(I), Y(II), ETR(I), ETR(II) and F _v/F _m of colonies formed from different branches showed the same trend: parameter values of first generation branches>second generation branches>third generation branches. These results suggest that the photosynthetic activities of different colonies of branches changed with the same trend. Furthermore, photosynthesis in G. lemaneiformis was found to be involved in vegetative reproduction and tetraspore formation. Finally, the first generation branches grew slowly, but accumulated organic compounds to form large numbers of tetraspores. Taken together, these results showed that the first generation branches are ideal materials for the release of tetraspores.     

Study on gametophyte vegetative growth ofUndaria pinnatifida and its applications

When cultured under certain environmental conditions (25°C, light intensity 80 μmol/m²·s, LD 12/12, in enriched seawater medium with 7×10⁻⁴ mol/L NO₃−N, 1.56×10⁻⁴ mol/L, PO₄−P and supplements of other elements like Mn, Fe, I, etc.), male and female gametophytes ofU. pinnatifida kept growing vegetatively and propagated fast at average daily fresh weight increase rate of about 20%. The empirical formulaG _m=G _o·3^m was established to estimate the output of vegetative gametophytes. Vigorous vegetative gametophyte cells began to form reproductive structures (oogonium and spermatangium, when the temperature was lower than 25°C and other environmental factors were kept optimal. The sufficient supply of gametophyte cells provided enough seeds for raisingUndaria sporelings on production scale. Controlled cross-breeding experiments using selected male and female gametophyte clones which increase their cell number by mitosis instead of meiosis were also carried out in vitro. Juvenile sporophytes from the cross-breeding had almost the same length and width increase rates as those of the control. The fact that vegetative gametophytes can be purposely selected, propagated quickly, cross-bred, with the resulting sporophytes having almost the same characteristics leads to a new way to select desiredUndaria strains for long time use without losing the desired economic characteristics. Contribution No. 2696 from the Institute of Oceanology, Chinese Academy of Sciences. Supported by National Natural Science Foundation of China.     

Early development of Costaria costata (C. Agardh) Saunders and cultivation trials

Costaria costata (C. Agardh) Saunders is one of common kelps distributed in many coastal areas worldwide; however, in China, no reports have been made on cultivation of the genus. To investigate potential cultivation of the species in the northern part of China, trials on isolation and preservation of the gametophytes were conducted using C. costata from Korea; growth and development of the gametophytes were observed. We showed that at 10±1°C, 60 μmol m⁻²s⁻¹ and 12:12 h (L:D), freshly released zoospores settled down within 1 hour, and then developed into the primary cell during the following 2 days. After a vegetative growth phase lasting 6–8 days, female gametophytes became 3–4 times larger in diameter than that of the primary cell, but still remained at a unicellular stage, while male gametophytes divided into 4–10 cells with only a slight change in size. Fertilization occurred within 10 days after the zoospores were released from the sporangia, and the apical and basal tissues of the juvenile sporophyte divided and differentiated into the blade and stipe. Temperature and irradiance influenced gametophytic vegetative growth and developmental patterns. Generally, low irradiance (15 μmol m⁻²s⁻¹ and 30 μmol m⁻²s⁻¹) was unfavorable to the induction of fertility, but it enhanced female gametophyte division. The optimal conditions for vegetative growth were 15°C and 30 μmol m⁻²s⁻¹. After transplantation of the juvenile seedlings and after eight months cultivation, the harvested mature blade reached 194 cm in length and 32.7 cm in width. Our study proves that it is feasible to implement propagation and large scale cultivation of C. costata in northern China.     

Light demands of juvenileLaminaria japonica

The light demands of seaweeds is an interesting and rather complex phenomenon because they depend not only on the species but also on their different development stages. Even different parts of the same plant sometimes have different light demands. Light control is an important procedure at large scaleLaminaria nursery stations in China. Technicians and scientists have different viewpoints on the best method to regulate light. A culture study onLaminaria japonica starting from zoospores to several centimeter sporophytes to find the optimal and critical irradiance ranges for juvenileLaminaria at different development stages added more knowledge on this aspect. Experiment results show gametophytes can not tolerate irradiance of more than 150 μE m⁻²s⁻¹ while sporophytes can tolerate more than 519 μE m⁻²s⁻¹. This big difference starts from the very early stage of 1-to 2-celled sporophytes. The biological basis and mechanism of this phenomenon need further research. Contribution No. 1515 from the Institute of Oceanology, Academia Sinica Contribution No. 1 from the Experimental Marine Biological Laboratory     

Distributions of sulfur pollutants in East Asia

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SEAWATER FLUX OF THE SOUTH CHINA SEA

The South China Sea water can be divided according to depth into three boxes by the pycnoclineand a sill.Using a box model with matter balance,the net seawater fluxes were calculated to be317.9×10~4 m~3/s in box Ⅰ for the upper homogeneous layer outflowing to the adjoining oceans;67×10~4 m~3/s in box Ⅲ for the water entering the basin;240×10~4 m~3/s in box Ⅱ for water entering theSouth China Sea.The upward speed of basin water was calculated to be 8.4×10~(-5) cm/s and that ofseawater flowing up along the pycnocline was calculated to be 8.9×10~(-5) cm/s.     

Standing crop and primary production of benthic microalgae on the tidal flats in Yueqing Bay

The standing stock and primary production of benthic microalgae on tidal flats were measured seasonally at 3 transects (Puqing, Dahengchuang and Puqi) in Yueqing Bay during 2002 2003. The results showed that the integral chlorophyll a (Chl a) concentration in tidal flat mud exhibited a seasonal variation with the order of magnitude: winter (14.0 4.2 mg m-2) > spring (13.0 6.3 mg m-2) > autumn (7.7 5.9 mg m-2) > summer (4.6 3.2 mg m-2). The primary production showed an order of magnitude: spring (270.5 224.9 mgC m-2 d-1)>winter (238.7 225.5 mgC m-2 d-1)>autumn (214.1 56.2 mgC m-2 d-1)>summer (71.6 44.6 mgC m-2 d-1). Both chlorophyll a and primary production showed maximum values in the surface layer of sediment, and decreased rapidly with increasing depth due to sun light limitation. The results of variance analysis indicated that seasonal variation and tidal flat condition affected Chl a greatly, but had no significant effect on primary production. The annual primary production of benthic microalgae on tidal flats in Yueqing Bay was estimated at 16143 tons carbon, which is sufficient to support 1.02×105 tons shellfish production. The environmental factors affecting chlorophyll and primary production on the tidal flats in Yueqing Bay were discussed. By comparing with other bays on China’s coast, it was observed that Yueqing Bay is a region with high benthic microalgae standing crop and primary production, which may be related to the type of its sediment.     

DETERMINATION OF TRACE BENZENE HYDROCARBONS IN JIAOZHOU BAY BY ENRICHMENT AND CAPILLARY COLUMN GAS CHROMATOGRAPHY

Trare amounts of benzene hydrocarbons obtained in Jiaozhou Bay (Qindao) were enriched bysorption on a GDX-102 column and eluted by carbon disulfide. The eluted was concenttaled and then de-temened by capillary column gas cbornatognphy.The contents of virious kinds of benzene hydrocarbons in Jiaozhou Bay coastal water were benzene(22.3-141.6)× 10～(-9)g/L, toluate (15.2-94.0) × 10~(-9) g/L, ethyl benzene(11.8-85.1)×10~(-9) g/L, p -xylene(15.2-78.5) ×10(-9) g/L, m-xylene (10.9-79.4) ×10(-9) g/L, o -xylene (12.4-80.1) x ×10(-9)g/L; iso-propyl(8.4- 73.1) x ×10(-9)g/L, n -propyl (6.9-76.4) ×10(-9) g/L, 1, 3, 5-trimethylbenzene (10.9- 35.9)×10(-9) g/L, 1,2, 4-trimethybenzene (10.0- 38.0)×10(-9) g/L, n - butydriare (8. 1 - 34.6) ×10(-9)g/L. The recovery of benzenehydrocarbons was (85.1 -95.6)%.     

Abundance and production ofBranchiura sowerbyi (Oligochaeta: Tubificidae) in two typical shallow lakes (Hubei, China)

An April 1996 to March 1997 comparative study on the abundance and secondary production ofBranchiura sowerbyi Beddard, 1892 in two typical shallow lakes showed that in Houhu, an algae-dominated lake, the worm density (68 ind·m⁻²) peaked in July, biomass (1.930 g·m⁻²) peaked in June, while in Biandantang, a macrophyte-dominated lake, standing stock (density: 60 ind·m⁻²; biomass: 1.019 g·m⁻² in wet weight) peaked in December. Secondary production of the animal in Houhu Lake was 3.413 g wet wt m⁻² a⁻¹, a little more than that (2.675 g wet wt m⁻² a⁻¹) in Biandantang Lake. Their turnover rates (P/B ratios) were 4.0 and 5.0, respectively. Project (39600019 and 39430101) supported by NSFC.     

Mixing in Shallow Waters： Measurements, Processing, and Applications

Microstructure profiling measurements taken on a shallow Black Sea shelf and in Lake Banyoles and Boadella reservoir (Both in Spain) are analyzed to investigate the influence of boundary-layer-induced turbulence of various sources on mixing in the water interior. The state of turbulence in shallow waters is examined and details of microstructure data processing and error analysis are discussed. The dependence between averaged activity parameter AG and buoyancy Reynolds number Reb for the shelf turbulence indicates that for Reb < 1 the state of turbulence can be described by the fossil turbulence model, which postulates AG-Reb1/2. For Reb> 1, however, the influence of Reb on AG is weak, signifying that the buoyancy Reynolds number can no longer serve as the governing parameter for active turbulent mixing. The generation of turbulence by a one-minute long wind bursts (the Boadella reservoir) increases the averaged dissipation rate (e) of the surface mixed layer by more than 5 times (up to 3×10-6 W kg-1). The influence of the wind bursts was also traced below the ther-mocline, where turbulent patches with     

Variation of bacteria biomass and its possible controlling factors in the East China Sea

Two surveys were performed for determining bacteria biomass (BB), temperature, salinity, chlorophyll a (chl-a) and nutrient concentrations at 11 stations with three sampling depths in the high-incidence regions of red tide in the East China Sea (ECS) in the spring of 2006. Temperature and salinity increased from nearshore to offshore region and from high latitude to low latitude in the two cruises of 2006. BB were between 0.3–5.2 mgC m⁻³ (about 2.1 mgC m⁻³ on average) and 0.2–6.0 mgC m⁻³ (about 2.7 mgC m⁻³ on average) respectively in the two cruises. BB in the surface layer decreased from the Changjiang River estuary to high sea and from low latitude to high latitude. The results showed that bacterial growth was regulated by temperature, primary production and inorganic nutrient concentrations depending on different hydrographic conditions. In the surface and middle layers where the primary production can supply enough organic substrate, temperature was the main factor to control bacteria biomass. BB showed a good correlation between the surface and middle layers in both cruises. The distribution of nutrients during both cruises showed a similar decreasing trend from nearshore region and high latitude to offshore region and low latitude. High BB values were mainly recorded from samples in the middle layer where chl-a concentrations were also high, indicating primary production being strongly correlated with temperature over the ECS shelf. In the offshore area, phosphate and silicate became limiting factors for phytoplankton growth with indirect influence on BB. Bacteria played an important role in nitrogen regeneration process turning organic nitrogen to inorganic forms such as NH₄ ⁺. The increasing ratio of NH₄ ⁺/DIN could be a proof of that.     

Annual production ofBranchiura sowerbyi (Oligochaeta: Tubificidae) in the Donghu Lake,Wuhan, China

Branchiura sowerbyi in the Donghu Lake (Wuhan, China) completes its life cycle in one year. Its production rates were 6.7 g m⁻² yr⁻¹ (wet weight) during the period from April 1962 to May 1963 and 8.6g m⁻² yr⁻¹ during the period from August 1963 to August 1964. The standing stocks in corresponding periods were 1.9g m⁻² (wet weight) and 1.1g m⁻², and theP/B ratios were 3.6 and 7.8. Based on the standing stock during 1973–1975, an evaluation of recent productin levels ofB. sowerbyi in Donghu Lake has also been made (i.e. 5.7–33.5g m⁻² yr⁻¹).     

DYNAMIC CHANGES OF INORGANIC NITROGEN AND ASTAXANTI—IIN ACCUMULATION IN HAEMATOCOCCUS PLUVIALIS

This study on dynamic changes of culture color,astaxanthin and chlorophylls,inorganic N including N-NO3^-,N-NO2^- and N-NH4^ in batch culture of Haematococcus pluvialis exposed to different additive nitrate concentration showed(1)ast/chl ratio was over 0.8 for brown and red algae,but was usually less than 0.5 for green and yellow algae;(2)N-NO3^-,in general,was unstable and decreased,except for a small unexpected increase in nitrate enriched treatment groups;(3)measurable amounts of N-NO2^- and N-NH4^ were observed respectively with three change modes although no extemal nitrite and ammonia were added into the culture;(4)a non-linear correlation between ast/chl ratio(or color)changes and the levels of N-NO3^-,N-NO2^-,N-NH4^ in H.pluvialis culture;(5)up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow to brown(or red)when N-NO3^-,N-NO2^- and N-NH4^ fluctuated around 30,5,5μmol/L respectively;(6)existence of three dynamic modes of N-NO3^-,N-NO2^- and N-NH4^ changes,obviously associated with initial extemal nitrate;(7)the key level of total inorganic N concentration regulating the above physiological changes during indoor cultivation was about 50 μmol/L;and(8)0.5-10mmol/L of nitrate was theoretically conducive to cell growth in batch culture.     

The South Pacific Subtropical Mode Water in the Tasman Sea

From the synopical CTD sections in the WOCE PR11 repeated cruises, the South Pacific Subtropical Mode Water (SPSTMW) has been identified in the region of the Tasman Front Extension (TFE) around 29?S to the east of Australia. In the depth range of 150-250 m, the SPSTMW appears as a thermostad with vertical temperature gradient lower than 1.6℃(100 m)-1 and a tem- perature range of 16.5-19.5℃ and as a pycnostad with PV lower than 2×10-10 m-1 s-1 and a potential density range of 25.4-26.0 kg m-3. Like the subtropical mode waters in the North Atlantic and North Pacific, the formation of the SPSTMW is associated with the convective mixing during the austral wintertime as manifested from the time series of the Argo floats. And cold water entrains into the mixed layer with the deepening mixed layer from September to the middle of October. During the wintertime formation process, mesoscale eddies prevailing in the TFE region play an important role in the SPSTMW formation, and have a great effect on the SPSTMW distribution in the next year. The deeper (shallower) mixed layer in wintertime, consistent with the depressed (uplifted) permanent thermocline, is formed by the anticyclonic (cyclonic) eddies, and the substantial mode water thicker than 50 m is mainly found in the region of the anticyclonic eddies where the permanent thermocline is deeper than 450 m.     

Studies on culture conditions of benthic diatoms for feeding abalone

Effects of salinity, pH, nitrogenous and phosphate nutrients on the growth rate of four species of benthic diatoms were studied by using the method of in situ optical density measurement. The optimal culture conditions for the four species of diatoms are as follows: forAmphora coffeaeformis, s 35; pH 8.5; NO₃ ⁻−N 1.54, NH₄ ⁺−N, 7.0; CO (NH₂)₂−N, 2.5 (mg/L); PO₄ ³⁻−P 1.13 (mg/L); forCocconeis scutellum varparva, s 30; pH 8.5; NO ₃ ⁻ −N 3.08, NH ₄ ⁺ −N 3.5, CO(NH₂)₂−N 5.0 (mg/L); pO ₄ ³⁻ −P 0.283 (mg/L); forNavicula corymbosa s 25; pH 8.0; NO ₃ ⁻ −N 1.54, NH ₄ ⁺ −N 3.5 CO(NH₂)₂−N 5.0 (mg/L); PO ₄ ³⁻ −P 0.565 (mg/L); forNavicula mollis. s 25; pH 8.0; NO ₃ ⁻ −N 1.54, NH ₄ ⁺ −N 1.75, CO(NH₂)₂−N 1.25 (mg/L); PO ₄ ³⁻ −P 0.141 (mg/L). Part of the results of this paper were exchanged in the Fourth Asian Aquaculture Forum. Oct. 16–20. 1995. Beijing, P. R. China.