金枪鱼油的精炼工艺对脂肪酸组成的影响

粗鱼油通过精炼可以除去非甘油三酯杂质成分，研究表明黄鳍金枪鱼鱼油精炼的工艺参数为：800g，L的H3PO4脱胶，添加量为油量的1％（体积分数）；300g／L的Na0H脱酸，添加量为油量的2％（体积分数）；活性土脱色。添加量为油量的10％（质量分数）；85℃下减压蒸馏脱臭15min。经过精炼，鱼油呈淡黄色，澄清透明，有淡鱼腥昧，理化指标达到鱼油SC/T3502—2000标准的精制鱼油一级要求，精制鱼油中PUFA的总含量（w）达37．06％，DHA和EPA含量（w）分别为25．10％和4．45％。     

南太平洋长鳍金枪鱼渔场预报模型研究

长鳍金枪鱼资源是南太平洋金枪鱼渔业的重要目标种类,也是我国金枪鱼延绳钓的主要捕捞对象之一。根据2008-2009年我国海洋渔业公司在南太平洋海域的生产数据,结合表层、105 m和205 m水层温度,以及海面高度、叶绿素a浓度等海洋环境数据,运用一元非线性回归方法,按季度建立基于各环境因子的长鳍金枪鱼栖息地适应性指数,采用算术平均法获得基于多环境因子的栖息地指数综合模型,并用于中心渔场的预报。通过与实际作业渔场的比较与验证,结果表明:模型预报准确性达到70%以上,具较高渔情预报准确度。     

南太平洋长鳍金枪鱼栖息地指数模型的比较研究

根据1994-2010年南太平洋长鳍金枪鱼生产统计数据,结合海表面温度(SST)和海面高度(SSH)等海洋环境数据,将投放钩数(捕捞努力量)作为适应性指数,采用外包络法,利用算术平均法(AM)、几何平均法(GM)、连乘法(CP)、最大值法(Max)和最小值法(Min)等5种方法建立栖息地指数(HSI)模型。研究表明,通过对这5种方法进行比较,最大值法、算术平均法和几何平均法均得到较好的拟合效果,其中最大值法拟合效果最好,模型的准确度可达到85.2%。研究认为,可基于海表面温度和海面高度等海洋遥感因子,利用最大值法栖息地指数模型,较好地预测南太平洋长鳍金枪鱼中心渔场。     

金枪鱼油的精炼工艺对脂肪酸组成的影响

粗鱼油通过精炼可以除去非甘油三酯杂质成分,研究表明黄鳍金枪鱼鱼油精炼的工艺参数为:800 g/L的H3PO4脱胶,添加量为油量的1%(体积分数);300 g/L的NaOH脱酸,添加量为油量的2%(体积分数);活性土脱色,添加量为油量的10%(质量分数);85℃下减压蒸馏脱臭15 min。经过精炼,鱼油呈淡黄色,澄清透明,有淡鱼腥味,理化指标达到鱼油SC/T3502-2000标准的精制鱼油一级要求,精制鱼油中PUFA的总含量(w)达37.06%,DHA和EPA含量(w)分别为25.10%和4.45%。     

东太平洋长鳍金枪鱼栖息地指数模型的比较

根据2009–2011年东太平洋海域长鳍金枪鱼延绳钓的生产数据,结合海洋遥感获得的表温(SST)和海面高度(SSH)的数据,采用外包络法,以投钩数、单位捕捞努力量渔获量(CPUE)为适应性指数,按月份分别建立基于SST和SSH的长鳍金枪鱼栖息地适应性指数(SI)。采用算术平均法(AMM)和几何平均法(GMM)获得基于SST和SSH环境因子的栖息地指数(HSI)综合模型,并以2012年各月生产数据进行模型验证。结果表明,基于两种HSI模型所得的长鳍金枪鱼栖息地综合指数不相同,通过算术平均法的HSI模型,当HSI为0.6以上时,渔获量占总渔获量的87.9%,通过几何平均法的HSI模型,当HSI为0.6以上时,渔获量所占比例为87.2%。算术平均法较GMM更适于东太平洋长鳍金枪鱼栖息地指数模型。     

黄鳍鲷细菌性溃疡病致病菌研究

研究了黄鳍鲷细菌性溃疡病的病原。从患病濒死的黄鳍鲷体内分离到两株细菌,经人工感染试验确定为致病菌,通过测定其形态及生理生化特性得出：致病菌为革兰氏阴性杆菌,极生单鞭毛,发酵葡萄糖产酸不产气,氧化酶阳性、过氧化氢酶阴性,发酵乳糖,甘露醇发酵阳性,明胶酶阳性,在70g／LNaCl培养基中生长良好,对O／129不敏感;由于两株菌对山梨醇、水扬苷、七叶苷及麦芽糖等氧化发酵的结果不一致,分别被鉴定为嗜水气单胞菌不产气亚种（Aeromonashydropilaanaerogenes）和温和气单胞菌（A．sobria）变异亚种。药敏试验显示,两个菌株对氯霉素高度敏感而对氟哌酸中度敏感。两株菌对黄鳍鲷的半致死剂量（按每g鱼计算）分别为2．3×106个菌体和2．6×105个菌体。     

基于GIS的印度洋大眼金枪鱼延绳钓钓获率与水温关系的研究

采用GIS定性分析和数值分析的方法,研究了印度洋大眼金枪鱼延绳钓钓获率与水温的 关系。结果表明,大眼金枪鱼延绳钓高钓获率的出现与印度洋加权水温大面分布存在明显的相互 关系,大眼金枪鱼的渔获适温在14～17℃间。建议测量200m水深处的水温作为海上生产时的参 考。     

黄鳍东方鲀精子的超微结构

在透射电子显微镜及环境扫描电子显微镜下观察黄鳍东方鲀精子的超微结构。结果表明:黄鳍东方鲀精子由头部和尾部两部分组成。头部呈长圆柱形,无顶体,核内有小空隙,空隙中有少量电子致密小颗粒存在;核后端正中有凹陷入核内的植入窝,中心粒复合体位于植入窝内,由近端中心粒、中心粒间体和基体组成;袖套位于核的后端,内含线粒体球和含有电子致密物质的小囊泡。尾部细长,自头部后端的正中部向后伸出,近核端部分很短,核心结构是轴丝,鞭毛起始于袖套腔,远核端有侧鳍;轴丝为典型的"9+2"微管对复合体结构。     

基于GIS的印度洋大眼金枪鱼延绳钓钓获率与水温关系的研究

采用GIS定性分析和数值分析的方法，研究了印度洋大眼金枪鱼延绳钓钓获率与水温的关系。结果表明，大眼金枪鱼延绳钓高钓获率的出现与印度洋加权水温大面分布存在明显的相互关系，大眼金枪鱼的渔获适温在14～17℃间。建议测量200m水深处的水温作为海上生产时的参考。     

黄鳍鲷生长激素cDNA的分子克隆和序列分析

生长激素 (ｇｒｏｗｔｈｈｏｒｍｏｎｅ,ＧＨ)是脊椎动物脑垂体细胞合成和分泌一种单链多肽激素 ,具有促进鱼类生长 ,加速蛋白质的合成 ,提高饲料转化率 ,调节海河洄游性鲑科鱼类的渗透压 ,促进鲑科鱼类对海水的适应能力 ,提高鱼类血液、腺体和肾脏中淋巴细胞的抗病毒能力 ,从而增强鱼体的抗感染能力等作用 ,被认为是最有效的生长促进剂之一 ,具有重要的潜在应用价值。目前 ,国内外已有40多种鱼类的ＧＨ基因被分离和克隆[1,2 ] ,序列分析表明 ,ＧＨ与泌乳刺激素 (ＰＲＬ)、胎盘催乳素(ＰＬ)、生长催乳素 (ＳＬ)具有相似的结构和交叉的功能 ,…     

Reproductive Biology of Yeilowfin Tuna T. albacares in the West-Central Indian Ocean

Survey of yellowfin tuna in the west-central Indian Ocean was conducted on board of Chinese Iongliners during 2003, 2004 and 2005, which is a part of Chinese Tuna Fishery Scientific Observer Program (CTFSOP). The reproductive biology has been investigated. A total of 1023 samples are collected including 417 ovaries and 606 testes. Spawning activities of yellowfin tuna have been studied for both male and female from January to June. The data showed that the average monthly sex ratio is 0.59, and the minimum length at sexual maturity is 101 cm for female and 110 cm for male respectively. Length at 50% sexual maturity is esti- mated at 113.77 cm for female and 120.20 cm for male, whereas maturation rate is 0.066 cm-1 for female and 0.091 cm-1 for male. Sex ratio by length class indicates that the proportion of male is higher than female's along with size increasing; for instance, in the group of the body length longer than 145 cm, some females have their body length from 145 to 160 cm and males have their body length at 160 cm and even longer. Statistically, yellowfin tuna has a significant seasonal reproduction.     

Reproductive biology of yellowfin tuna T. albacares in the west-central Indian Ocean

Survey of yellowfin tuna in the west-central Indian Ocean was conducted on board of Chinese longliners during 2003, 2004 and 2005, which is a part of Chinese Tuna Fishery Scientific Observer Program (CTFSOP). The reproductive biology has been investigated. A total of 1 023 samples are collected including 417 ovaries and 606 testes. Spawning activities of yellowfin tuna have been studied for both male and female from January to June. The data showed that the average monthly sex ratio is 0.59, and the minimum length at sexual maturity is 101 cm for female and 110 cm for male respectively. Length at 50% sexual maturity is estimated at 113.77 cm for female and 120.20 cm for male, whereas maturation rate is 0.066 cm⁻¹ for female and 0.091 cm⁻¹ for male. Sex ratio by length class indicates that the proportion of male is higher than female’s along with size increasing; for instance, in the group of the body length longer than 145 cm, some females have their body length from 145 to 160 cm and males have their body length at 160 cm and even longer. Statistically, yellowfin tuna has a significant seasonal reproduction.     

Evaluation of three harvest control rules for Bigeye Tuna (Thunnus obesus) fisheries in the Indian Ocean

The stock of Bigeye tuna(Thunnus obesus) in the Indian Ocean supports an important international fishery and is considered to be fully exploited. The responsible management agency, the Indian Ocean Tuna Commission(IOTC), does not have an explicit management decision-making framework in place to prevent over-fishing. In this study, we evaluated three harvest control rules, i) constant fishing mortality(CF), from 0.2 to 0.6, ii) constant catch(CC), from 60000 to 140000 t, and iii) constant escapement(CE), from 0.3 to 0.7. The population dynamics simulated by the operating model was based on the most recent stock assessment using Stock Synthesis version Ⅲ(SS3). Three simulation scenarios(low, medium and high productivity) were designed to cover possible uncertainty in the stock assessment and biological parameters. Performances of three harvest control rules were compared on the basis of three management objectives(over 3, 10 and 25 years): i) the probability of maintaining spawning stock biomass above a level that can sustain maximum sustainable yield(MSY) on average, ii) the probability of achieving average catches between 0.8 MSY and 1.0 MSY, and iii) inter-annual variability in catches. The constant escapement strategy(CE=0.5), constant fishing mortality strategy(F=0.4) and constant catch(CC=80000) were the most rational among the respective management scenarios. It is concluded that the short-term annual catch is suggested at 80000 t, and the potential total allowable catch for a stable yield could be set at 120000 t once the stock had recovered successfully. All the strategies considered in this study to achieve a ‘tolerable' balance between resource conservation and utilization have been based around the management objectives of the IOTC.     

Evaluation of FAD-associated purse seine fishery reduction strategies for bigeye tuna (Thunnus obesus) in the Indian Ocean

In the Indian Ocean, bigeye tuna supports one of the most important fisheries in the world. This fishery mainly consists of two components: longline and purse seine fisheries. Evidence of overfishing and stock depletion of bigeye tuna calls for an evaluation of alternative management strategies. Using an age-structured operating model, parameterized with the results derived in a recent stock assessment, we evaluated the effectiveness of applying constant fishing mortality (CF) and quasi-constant fishing mortality (QCF) strategies to reduce fishing effort of purse seining with fish aggregating devices (FADs) at different rates. Three different levels of productivity accounted for the uncertainty in our understanding of stock productivity. The study shows that the results of CF and QCF are similar. Average SSB and catch during simulation years would be higher if fishing mortality of FAD-associated purse seining was reduced rapidly. The banning or rapid reduction of purse seining with FAD resulted in a mean catch, and catch in the last simulation year, higher than that of the base case in which no change was made to the purse seine fishery. This could be caused by growth overfishing by purse seine fisheries with FADs according to the per-recruit analysis. These differences would be more obvious when stock productivity was low. Transferring efforts of FAD-associated purse seining to longline fisheries is also not feasible. Our study suggests that changes are necessary to improve the performance of the current management strategy.     

多变量分位数回归构建印度洋大眼金枪鱼栖息地指数

以0～300m水层加权平均水温、50～150m水层的温差和氧差及其交互变量为影响因子,运用分位数回归法,寻找出环境变量与大眼金枪鱼(Thunnus obesus)延绳钓钓获率的最佳上界分位数回归方程,计算出栖息地指数(HSI),并应用地理信息系统(GIS)软件绘制各月HSI空间分布图。研究表明:大眼金枪鱼延绳钓钓获率(HR)依加权平均水温(x)、温差(y)、氧差(z)与的最佳上界分位数回归方程为HR0.70=-15.596+2.124x-0.003x3+0.033xyz-0.036y2z+0.107yz2-0.337z3;HSI空间分布为:16°S—10°N印度洋海域HSI高于0.7,HSI>0.8的海域随季节发生显著变化,马达加斯加外海至100°E、16°S—26°S海域常年存在一片HSI<0.4的区域,26°S—40°S海域的HSI介于0.4～0.5,40°S以南海域HSI<0.4,东非外海季节性地出现一片HSI<0.6的海域。利用多个环境变量的栖息地指数模型来预测分析大洋金枪鱼资源分布效果较好。     

Variations in the eastern Indian Ocean warm pool and its relation to the dipole in the tropical Indian Ocean

Based on the monthly average SST and 850 hPa monthly average wind data,the seasonal,interannual and long-term variations in the eastern Indian Ocean warm pool(EIWP) and its relationship to the Indian Ocean Dipole(IOD),and its response to the wind over the Indian Ocean are analyzed in this study.The results show that the distribution range,boundary and area of the EIWP exhibited obviously seasonal and interannual variations associated with the ENSO cycles.Further analysis suggests that the EIWP had obvious l...     

Modeling the hook depth distribution of pelagic longlining in the equatorial area of Indian Ocean

A survey was conducted in the equatorial area of Indian Ocean for a better understanding of the dynamics of hook depth distribution of pelagic longline fishery.We determined the relationship between hook depth and vertical shear of current coefficiency,wind speed,hook position code,sine of wind angle,sine of angle of attack and weight of messenger weight.We identified the hook depth models by the analysis of covariance with a general linear model.The results showed that the wind effect on the hook depth can be ignored from October to November in the survey area;the surface current effect on the hook depth can be ignored;the equatorial undercurrent is the key factor for the hook depth in Indian Ocean;and there is a negative correlation between the hook depth and vertical shear of current and angle of attack.It was also found that the deeper the hook was set,the higher hook depth shoaling was.The proposed model improves the accuracy of the prediction of hook depth,which can be used to estimate the vertical distribution of pelagic fish in water column.     

Influence of the Eastern Indian Ocean Warm Pool Variability on the Spring Precipitation in China

The relationship between the variability of the Eastern India Ocean Warm Pool （EIWP） and the spring precipitation in China is studied in the paper based on an analysis of the Simple Ocean Data Assimilation （SODA） Sea Surface Temperature （SST） data, the reanalysis data of monthly grid wind field at 925 hPa with a resolution of 2.5^＊ latitude and longitude from the National Center for Environmental Prediction/National Center for Atmospheric Research （NCEP/NCAR）, and the monthly mean rainfall data from 160 observational stations in China. The results show that there is a strong correlation between the EIWP variability and the spring precipitation in China. The area, volume and intensity indices of the EIWP are negatively correlated with the spring precipitation in southwestern China, while they are positively correlated with the spring precipitation in the rest of China, especially in the northeast. For this correlation between the EIWP variability and the spring precipitation in China, it is found that the correlative relationship is mainly connected with the variations of the moisture transport by the warm air flow, which is under the influence of the EIWP variability, into the inland of China in spring. Two causative factors may influence this transport. One is the variation of the moisture transport carried by the warm air flow from the Arabian Sea influenced by the EIWP variability. The other is the variation of the equator-crossing flow （70^＊-90^＊E） influenced by the EIWP anomaly in the previous winter which exerts its effect on the moist warm air transported from the Southern Hemisphere. The position and intensity of the Western North Pacific Subtropical High （WNPSH） variability caused by EIWP variation also influence the spring precipitation in China.     

Contrast between the climatic states of the warm pool in the Indian Ocean and in the Pacific Ocean

Based on the analysis of Levitus data, the climatic states of the warm pool in the Indian Ocean (WPIO) and in the Pacific Ocean (WPPO) are studied. it is found that WPIO has a relatively smaller area, a shallower bottom and a slightly lower seawater temperature than those of WPPO. The horizontal area at different depths, volumes, central positions, and bottom depths of both WPIO and WPPO show quite apparent signals of seasonal variation. The maximum amplitude of WPIO surface area’s seasonal variation is 58% larger over the annual mean value. WPIO’s maximum volume variation amplitude is 66% larger over the annual mean value. The maximum variation amplitudes of the surface area and volume of WPPO are 20.9% and 20.6% larger over the annual mean value respectively. WPIO and WPPO show different temporal and spatial characteristics mainly due to the different wind fields and restriction of ocean basin geometry. For instance, seasonal northern displacement of WPIO is, to some extent, constrained by the basin of the Indian Ocean, while WPPO moves relatively freely in the longitudinal direction. The influence of WPIO and WPPO over the atmospheric motion must be quite different.