Beeinflussung der Enzymaktivität einer Protease durch Huminstoffe Influence of Humic Substances on the Activity of a Protease

This paper deals with the influence of different humic substances (HS) on the enzyme activity (EA) of the proteolytic enzyme pronase E. The EA was determined by analyzing the concentration of the amino acid valine hydrolyzed from caseine. For amino acid analysis, a flourescence detector was used after precolumn derivatization and HPLC-separation of the hydrolysis products. The data show that the presence of HS has no adverse effects on amino acid analysis. Based on this result, a decrease of EA was observed in the presence of HS acting as enzyme inhibitors. Clear inhibition effects could be seen for different fulvic acids (HO3 FA, FBR FA and FBR 1.0 FA) at concentrations of 150 mg/L. In case of FBI-2 FA, higher concentrations were necessary to decrease the EA significantly. At an inhibitor concentration of 1500 mg/L the EA was mostly found to be less than 50%. The standard deviations of the results don't allow a differentiation between the HS of different origin and treatment. Specifying HS according to their influence on EA has to remain uncertain. As shown in a kinetic study of the hydrolysis of L-Leucine-2-naphthylamide-hydrochloride, the inhibitor HO3 FA decreases the maximum velocity of the reaction but has no effect on the Michaelis constant. These results indicate that this HS acts as a noncompetitive inhibitor.     

鳑鲏对“蚌-草”关系的影响

重建沉水植物群落是修复浅水富营养化湖泊的关键.河蚌可改善水下光照条件、促进沉水植物生长,因此放养河蚌常被用于沉水植物群落恢复的并行手段.河蚌是鳑鲏产卵的重要基质,因此河蚌可能促进鳑鲏种群发展,而鳑鲏对水生态系统的影响还尚不清楚.本研究以密刺苦草、大鳍鱊和背角无齿蚌为研究对象,通过中宇宙试验研究河蚌和鳑鲏对附着藻的影响,以及鳑鲏对水质和沉水植物生长的影响及机理.结果发现：鳑鲏显著增加了水体总磷、总溶解性磷、总悬浮物和叶绿素a浓度,而对总氮和总溶解性氮浓度的影响不显著.河蚌对苦草的相对生长率、总株数、根冠比及苦草最大叶长均无显著影响,而显著增加了苦草的单株平均生物量,这可能与河蚌组较高的附着藻生物量有关.鳑鲏未显著影响河蚌与苦草间的关系,但鳑鲏的出现显著增加了附着藻类生物量;此外,鳑鲏还降低了苦草的根冠比,而增加了苦草的最大叶长,这可能与鳑鲏引起的营养盐和叶绿素a浓度升高,以及植物表面附着藻生物量显著升高有关.鳑鲏属于小型杂食性鱼类,在长江中下游地区分布广泛,与沉水植物关系密切,且易在修复后的湖泊中形成优势鱼类,因此在湖泊修复和管理中应加强此类小型杂食性鱼类的监测与管理.     

2017—2021年长江天鹅洲故道鱼类群落结构变化、成因分析及对长江江豚迁地保护的启示

长江天鹅洲故道是长江江豚首个迁地保护区,其中充足而适口的饵料鱼类资源是长江江豚种群健康发展的保障。为此,根据2017年10月—2021年4月对长江天鹅洲故道进行的7次鱼类调查数据,结合禁捕前的历史数据,对其鱼类群落结构演变进行了分析,并针对长江江豚饵料鱼的供应状况对天鹅洲故道内鱼类资源管理提出了建议。调查期间,共采集到鱼类57种,其中,蒙古鲌(Culter mongolicus)、鲢(Hypophthalmichthys molitrix)和鳙(Aristichthys nobilis)等大中型鱼类生物量明显增加,而?(Hemiculter leucisculus)和似鳊(Pseudobrama simoni)等小型鱼类生物量呈现下降趋势。鱼类群落优势种逐渐由太湖新银鱼(Neosalanx taihuensis)、短颌鲚(Coilia brachygnathus)和?等小型鱼类转变为鳙、蒙古鲌和拟尖头鲌(Culter oxycephaloides)等大中型鱼类,尤其是新近出现的拟尖头鲌种群发展迅速。同时,短颌鲚和?等小型鱼类的体重在减小,而鲢、鳙等大中型鱼类规格在持续提高。长江江豚以小...     

Fischökologische Leitbilder fünf ausgewählter Salzkammergutseen

Ecological base line states for fish communities are necessary for the evaluation of ecological integrity. In Austria the fish communities of all the larger lakes are strongly influenced by human activities, like commercial fisheries, fish stocking, eutrophication or shore line degradation, and therefore these baseline states can not be developed by comparison with a natural, undisturbed lake. We developed ecological baseline states for the fish communities of the lakes Hallstättersee, Traunsee, Mondsee, Irrsee and Wallersee by reconstructing the native fish communities of these lakes from historical documents (from between 1500 to 1940). Then we classified the potential fish species of these lakes according to their ecological requirements. Finally we developed the base line states with 16 different ecological factors similar to the factors used for the ecological integrity assessment procedure for streams.

The process of reconstructing the fish communities and some advantages and disadvantages of the base line states for fish communities are discussed.     

内蒙古锡林郭勒盟盐湖浮游生物的群落特征

通过2003年9月-2004年1月的渔获物调查,并结合1984-2002年的鱼类产量统计资料,分析了近二十年来太湖鱼类产量和组成的变化规律以及环境间的相互作用关系.结果表明,太湖鱼类的种类在不断减少,如本次调查和2002年3月-2003年12月的调查只采集到48种鱼类,和历史资源料比较有55种鱼类难以采到;鱼类产量组成表现为仅鲚鱼的产量和组成比例总体呈较快的上升趋势,从1984年的5153.7t、占总产量的36.2%上升到2002年的19571t、64.1%,鲤、鲫鱼基本维持不变,但近四年略有上升,其余鱼类均呈下降趋势.用Wilhm改进式计算的鱼类产量组成的均匀度指数H总体呈逐年下降趋势,近二十年来平均以每年-0.0465的速率下降;分析认为,人类活动以及由此引起的太湖生态环境变化,包括江湖阻隔、过渡捕捞、富营养化及太湖特有的鱼类生态学特点决定了鱼类组成的这一变化趋势:江湖阻隔、过度捕捞是引起太湖鱼类种类减少和鱼类组成发生巨大变化的主要原因;太湖近二十年来富营养化程度不断加重,由此使初级生产力水平持续增长,浮游生物饵料丰富,使鲚鱼等浮游生物食性鱼类产量逐年提高;舶鱼数量的减少又使鲚鱼失去抑制.随着鲚鱼等浮游动物食性鱼类产量的升高,太湖浮游动物的生物量呈下降趋势,这可能是太湖浮游植物数量倍增的生物学因素之一.目前太湖鱼类产量组成的变化正向着抑制浮游动物和有利浮游植物生长的方向发展.     

Effects of fish manipulation on the plankton community in small hypertrophic lakes from the Pampa Plain (Argentina)

Trophic cascade hypotheses predict that fish will affect the structure and biomass of pelagic plankton communities. In order to investigate trophic cascade effects from fish down to phytoplankton, whole-lake studies were performed in five hypertrophic (mean total phosphorus (TP) concentrations higher than 1000 mg m⁻³) shallow lakes located in the Pampa Plain. The main climatic characteristic of this region is the alternation between periods of drought and flood, with corresponding changes of lake depth and conductivity of lake water. All lakes were studied from April to December 2000. Samples were taken of their physical and chemical characteristics and biotic communities, focusing on the zooplankton community. Fish were manipulated in four lakes (Capurro, Longinotti, Vedia 1, Vedia 2), while the fifth (Lake Vedia 3) was left undisturbed as a reference system. High abundance of planktivorous minnows (Jenynsia multidentata and Cheirodon interruptus) dominated the fish community in the reference lake. In the manipulated lakes, fish stocks were largely reduced in late autumn (May). During winter, Capurro, Longinotti and Vedia 1 were stocked with a visual planktivore, the pampean silverside (Odontesthes bonariensis, Atherinidae). Fish stocking was 24, 33 and 19 kg ha⁻¹, respectively. In contrast, no fish were stocked in Lake Vedia 2. Following fish removal, large Daphnia appeared in these lakes and grazed intensively on the phytoplankton. In contrast, no Daphnia were found in the reference lake (Vedia 3). The stocking of O. bonariensis in lakes Capurro, Longinotti and Vedia 1 led to a decrease in the percentage of large cladocerans and a rise in the phytoplankton biomass:TP ratio. Moreover, the lakes mentioned were stocked with different quantities of silversides over different periods of time. These differences were reflected temporarily in the plankton dynamics, affecting mainly larger sized zooplankton. Nevertheless, the presence of Daphnia was short lived in the lake where fish had been removed and no O. bonariensis were stocked. Competition for resources and recruitment of remaining fish probably caused a collapse in the zooplankton biomass. Our results support the idea that fish predation on zooplankton and its effect on phytoplankton is very intense in small pampean lakes. Fish removal was short lived, however. This could be because in small pampean lakes fish recolonization is favored, and minnows are highly prolific. Moreover, if manipulation of the trophic structure of lakes is undertaken in the pampean region, high nutrient loading from the watershed, climate and hydrology should also be taken into account.     

千岛湖(新安江水库)4种同域共存的鱼食性鱼类生活史策略比较研究

千岛湖(新安江水库)是我国大水面生态渔业发展的典型湖库,其渔业主要以鲢、鳙等滤食性鱼类增殖为主,对鱼食性鱼类资源的研究和关注相对匮乏。而鱼食性鱼类是水生食物网的顶级捕食者,对维持水生态系统结构和功能具有重要作用。近年来,受过度捕捞、生境退化等影响,千岛湖鱼食性鱼类资源下降明显。鉴于此,2021年3月—2022年12月,逐月在千岛湖收集4种同域共存的鱼食性鱼类样品1032尾,包括翘嘴鲌(Culter alburnus)、蒙古鲌(Culter mongolicus)、大眼鳜(Siniperca knerii)和斑鳜(Siniperca scherzeri),系统研究并比较了4种鱼食性鱼类年龄、生长、繁殖等生活史策略的分化特征。结果表明,千岛湖4种鱼食性鱼类优势年龄组均未超过3龄,说明其种群呈现低龄化现象。生长式型上,翘嘴鲌和蒙古鲌属正异速生长,而大眼鳜和斑鳜属负异速生长,说明4种鱼食性鱼类具有不同的生长策略。条件系数(CF)的变化趋势与繁殖活动密切相关,其中大眼鳜的条件系数最高(1.92%±0.03%),其次为斑鳜(1.72%±0.02%)和蒙古鲌(1.14%±0.01%),翘嘴鲌的条件系...     

The organisation of fish assemblages in the regulated Lima basin, Northern Portugal

In order to understand the structure of fish assemblages in the modified Lima basin (Northern Portugal), two distinct datasets concerning the presence and abundance of fish species were subjected to multivariate analysis. On the River Lima two types of flow modification are present within kilometres of one another: (a) a reduced and constant flow due to hypolimnetic release; and (b) an intense and irregular flow. A comparison of their influence on fish assemblages revealed a gradient of assemblage types from tributaries to main river sites. The latter were characterised by a strong dominance of cyprinids, particularly Iberian barbel (Barbus bocagei). The former harboured two kinds of fish assemblages: those closer to the river mouth were dominated by the cyprinids Iberian chub (Squalius carolitertii) and Iberian nase (Chondrostoma polylepis), which were also frequently present in the main river; while in those further upstream the predominant species was the brown trout (Salmo trutta). Although explanatory variables such as distance from source, altitude, substrate coarseness and width were the primary correlates of fish assemblage composition, dam construction and flow regulation also had a significant effect upon assemblage structure, particularly by: i) reducing the importance of migratory species; ii) constraining the presence of trout in the regulated segments; and iii) simplifying the community, especially in the case of the constant and reduced flow regime.     

湖南沅水五强溪水库鱼类资源现状及其历史变化

水电工程对鱼类生境和资源多样性具有重大的影响.于2012年4月-2014年3月,对湖南省沅水五强溪水库鱼类资源进行调查,以了解五强溪水库建成20年后鱼类资源的现状及多样性变化.结果表明,在五强溪水库共采集鉴定鱼类86种(包括引进种5种),隶属7目18科58属.种类组成以鲤形目种类数最多,占总种类数的66.28%.在五强溪水库库尾、库区和坝下江段,鱼类物种数分别为54、67和52,其中,库区与坝下江段之间鱼类群落为中等相似,库区与库尾、库尾与坝下江段之间鱼类群落为中等不相似.在生态类型上,水库鱼类呈现多样化特征,以湖泊定居性、杂食性和底栖性鱼类居多,分别占总种类数的68.60%、55.81%和41.86%.各江段渔获物组成存在一定差异,但黄颡鱼(Pelteobagrus fulvidraco)、鲤(Cyprinuscarpio)、鲫(Carassius auratus)、蒙古鲌(Culter mongolicus)等在各江段均占有较高比例.渔获物产量和规格以库区较高,库尾、坝下江段偏低,鱼类小型化现象明显.与五强溪水库建成前相比,本次调查水库库区江段鱼类种类减少46种,降幅为40.71%,一些常见经济鱼类如鳤(Ochetobius elongatus)、白甲鱼(Onychostoma sima)、瓣结鱼(Brevifilis brevifilis)、长吻鮠(Leiocassis longirostris)等没有发现.     

低头坝养鱼对上下游局域栖息地和鱼类群落时空格局的影响

河流鱼类的群落特征会随栖息地环境的变化而发生变化.为了解鱼类群落对低头坝养鱼的响应程度,2013—2014年共6次调查了乌龙河(长江下游支流)两个可涉水河段样点(上游坝和下游坝)的环境因子、鱼类多样性及其群落结构.共采集到5目10科21属24种鱼类,以鲤形目鲤科最多,占总数的58.3%.通过解析时空动态变化与局域栖息地条件对鱼类群落的影响,发现季节因素对鱼类群落结构无显著影响,而鱼类群落所在空间位置(上游坝,下游坝)对群落结构差异具有较显著影响,其中上游中华鳑鲏(Rhodeus sinensis)、(Hemiculter leucisculus)、棒花鱼(Abbottina rivularis)、大鳍鱊(Acheilognathus macropterus),下游食蚊鱼(Gambusia affinis)的多度决定了这种差异;而3个栖息地变量(溶解氧浓度、电导率和流速)均对鱼类群落特征具有显著性影响.栖息地环境差异对河流上、下游鱼类群落的影响较大,具体体现在下游样点鱼类的捕获重量、捕获数量、密度、多样性明显下降,推测利用低头坝养鱼是造成这些现象的重要原因.     

蓄水后向家坝库区鱼类物种、分类和功能多样性变化

为了解向家坝水电工程对金沙江下游鱼类多样性的影响,基于2016—2018年邵女坪(库首)、绥江(库中)和桧溪(库尾)3个江段的鱼类群落调查数据,分析了向家坝库区鱼类物种、分类和功能多样性空间格局,结合蓄水前(2011年)和蓄水初期(2015年)的资料,探讨了蓄水后向家坝库区不同维度鱼类多样性的变化趋势。向家坝库区各江段以广适性的瓦氏黄颡鱼(Pelteobagrus vachelli)和静水性的(Hemiculter leucisculus)占优势,两者的数量百分比分别为30.15%±14.82%和13.91%±11.16%。非度量多维尺度分析显示,蓄水后向家坝库区库首邵女坪和库中绥江鱼类群落差异最小,库首邵女坪和库尾桧溪鱼类群落差异最大;库中绥江在蓄水前、蓄水初期和2016—2018年3个时期的鱼类群落差异均较大,表明蓄水后绥江江段鱼类群落经历了剧烈变化。库区不同江段鱼类Margalef丰富度指数、Shannon-Wiener多样性指数和Pielou均匀度指数均表现为:桧溪>邵女坪>绥江,鱼类分类多样性指数和分类差异指数均表现为:桧溪>绥江>邵女坪。库区鱼类摄食、运动和繁殖等不同维度的功能多样性(功能丰富度、功能均匀度、功能离散度)空间格局不同,但总体上库中绥江鱼类功能丰富度最高,功能均匀度最低。与蓄水前相比,绥江江段鱼类物种、分类多样性呈下降趋势,鱼类功能丰富度和均匀度也有下降趋势。蓄水后绥江江段鱼类群落主要功能性状发生了明显变化,如蓄水前以下位口、纺锤形体型鱼类占优势,蓄水后以端位口、侧扁形体型鱼类占优势。不同维度多样性指数侧重于鱼类多样性的不同方面,结合多种指数分析有利于更全面地了解鱼类群落对环境变化和外界干扰的响应。研究结果有助于深入了解水电工程建设对金沙江下游鱼类多样性的影响,为鱼类多样性保护提供依据,也可为长江“十年禁渔”效果评估提供本底资料。     

金沙江下游支流黑水河松新电站幼鱼下行过坝研究

梯级小水电的开发一定程度上破坏了河流连通性并阻碍了鱼类洄游通道。黑水河流域松新坝址为了满足鱼类上行产卵修建了松新鱼道,然而作为洄游物种生命周期中的关键行为,鱼类下行洄游的需求也同等重要。为了寻求一种可行、高效、低损伤的下行过鱼方式,了解河流中不同环境因素对鱼类下行效率的影响,本研究在金沙江下游支流黑水河松新水电站补水通道开展鱼类下行过坝效果评估研究。针对两种鲤科鱼类(棒花鱼Abbottina rivularis和短须裂腹鱼Schizothorax wangchiachii)幼鱼和两种鳅科鱼类(短体副鳅Paracobitis potanini和红尾副鳅Paracobitis variegatus)幼鱼各放鱼90尾,定量分析鱼种、水体透明度、放鱼位置、流量、水温、水位等因素对鱼类下行效率的影响。结果表明:棒花鱼、短须裂腹鱼、短体副鳅、红尾副鳅的下行率依次为22.22%(20尾)、28.89%(26尾)、8.89%(8尾)、15.56%(14尾);幼鱼下行成功率与上游河道水温呈正相关趋势,且放鱼位置、鱼种、上游河道水温和流量均对鱼类下行成功率有显著影响。通过构建二元Logistic回归模型识...     

云南云龙天池枝角类群落演替对1950s以来人类活动影响信号的识别

从1950s开始,云南地区部分湖泊受到了水文调控(如筑坝)和鱼类引入等流域开发活动的直接影响,湖泊水环境与生态系统结构已出现明显改变.开展浮游动物群落的长期生态响应评价有助于认识气候波动和人为胁迫的影响模式.本文选取云龙天池进行了沉积物记录分析,在重建过去100年环境变化历史的基础上开展了枝角类群落的多指标分析(物种组成、个体大小、生物量等),进一步结合多变量分析识别了枝角类群落构建的关键驱动因子.结果显示,云龙天池在过去100年间经历了明显的水位波动,约1962年以前水位较低,1950s起的水文调控(筑坝)导致湖泊水位波动上升,2006年以来略有下降.枝角类群落随水位波动呈现由底栖种向浮游种占优转变的模式,并在2006年以来底栖种略有增加.总体上,低水位时期底栖枝角类占优,高水位时期浮游枝角类占优.水体营养水平也对枝角类群落产生了较为显著的影响.在沉积物总氮和有机质通量上升时,长额象鼻溞(Bosmina longirostris)相对丰度和枝角类浓度都明显上升.枝角类象鼻溞个体大小表明,1969年鱼类引入后象鼻溞的壳长、壳刺长度、触角长度显著减小,反映了鱼类捕食压力上升的影响.本研究...     

基于水声学探测的镜泊湖鱼类时空分布特征及资源量评估

2020年5、7和10月,采用多网目复合刺网调查和水声学探测相结合的方式对镜泊湖鱼类群落时空动态和资源量进行调查．渔获物调查共采集鱼类4目6科29种,其中鲤科鱼类最多,主要经济鱼类为鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)、蒙古鲌(Chanodichthys mongolicus)、瓦氏雅罗鱼(Leuciscus waleckii)、鲤(Cyprinus carpio)、翘嘴鲌(Culter alburnus)．蒙古鲌和■(Hemiculter leucisculus)为优势种．水声学探测结果表明,镜泊湖鱼类密度在春季、夏季和秋季分别为836、7216和2724 ind．/hm²,其中夏季鱼类密度最高且主要集中于中部和南部区域,春季和秋季鱼类密度则呈现从南至北递减的趋势．春、夏、秋季鱼类的平均目标强度为-43.4、-50.0和-46.5 dB,估算的平均全长分别为34.0、18.0和25.2 cm,推算的平均体重分别为272.7、43.7和135.2 g．结合各季节平均密度,估算出春、夏、秋季镜泊...     

鄱阳湖网箱养殖区沉积物有机质来源分析

通过对鱼苗时期鄱阳湖网箱养殖区沉积物、饵料及鱼粪等样品总有机碳(TOC)含量、总氮(TN)含量、碳氮比(C/N)、δ^13 C及δ^15 N的测定,分析探讨了鄱阳湖网箱养殖区沉积物有机质来源,量化了网箱养殖废物对养殖区沉积物有机质的贡献.结果表明,网箱养殖区沉积物的δ^13 C和δ^15 N值分别为-27.67‰~-25.65‰和5.19‰~7.27‰,饵料的δ^13 C和δ^15 N值分别为-24.73‰和10.28‰,鱼粪的δ^13 C和δ^15 N值分别为-26.30‰和15.54‰.网箱养殖区沉积物有机质来源主要有残饵、浮游生物及其他来源,其贡献率分别为48.3%±11.4%、25.6%±11.3%及26.0%±5.8%,而鱼粪的贡献几乎可以忽略不计.在水动力平流引起的扩散及沉积物的再悬浮的影响下,网箱养殖源有机质的扩散距离达1500 m.在鱼苗时期,鱼类网箱养殖的残饵是鄱阳湖网箱养殖区沉积物有机质的主要来源.     

The influence of pearl oyster farming on reef fish abundance and diversity in Ahe,French Polynesia

Many cultured pearl farms are located in areas of the Pacific that have thriving, highly diverse fish communities but the impacts of farming on these communities are poorly understood. We studied the effects of pearl oyster farming on shore fish abundance and diversity in the lagoon of Ahe, French Polynesia by adapting roving diver census methods to the coral reef bommies of the lagoon and compared 16 sites with high pearl farming impact to others with no direct impact. Pearl farming has a slightly positive effect on reef fish abundance (N) and no significant impact on fish diversity (H) or community composition. This is important when considering the ecological sustainability of pearl farming in French Polynesia and suggests that a potential synergy between pearl farms and marine conservation should be further explored.     

我国水库综合养鱼的发展前景

本文从分析水库综合养鱼生态系统的生态关系入手,阐述了水库综合养鱼的内容、意义、理论依据以及发展水库综合养鱼的自然条件和实践基础,并对综合养鱼系统的鱼产潜力进行了预测。预测结果表明,水库发展综合养鱼比传统的粗养方式其单位面积鱼产量可获得大幅度提高。