The roles of serine protease, intracellular and extracellular phenoloxidase in activation of prophenoloxidase system, and characterization of phenoloxidase from shrimp haemocytes induced by lipopolysaccharide or dopamine

We investigated the effects of lipopolysaccharide(LPS) and dopamine(DA) on the activation of the prophenoloxidase(proPO) system of Litopenaeus vannamei.LPS and DA were shown with a negative dose-dependent effect on hyalne cells(HC),semi-granular cells(SGC),large granular cells(LGC),and total haemocyte count(THC).When haemocytes were treated with LPS or DA,serine proteinase activity and intracellular phenoloxidase(PO) activity were significantly reduced,but extracellular PO activity increased significantly.These findings indicated that the reduction in haemocyte counts was mainly because of the degranulation and activation of the proPO system from semi-granule and large granule cells.The PKC inhibitor,chelerythrine,and the TPK inhibitor,genistein,had an inhibitory effect on extracellular PO activity,while serine proteinase and intracellular PO activity increased.This suggests that the LPS and DA induce the activation of proPO in haemocytes via PKC and TPK-related signaling pathways,but serine proteinase may be activated only by PKC,as the genistein effects were not statistically significant.Electrophoresis analysis revealed that POs induced by LPS or DA have the same molecular mass and high diphenolase activity.Two PO bands at 526 kDa and 272 kDa were observed in PAGE,while in the haemocyte lysate supernatant(HLS),only a 272-kDa band was observed.This band was resolved after SDS-PAGE under non-reducing and reducing conditions into two groups of POs,166 kDa and 126 kDa,and 78.1 kDa and 73.6 kDa,respectively,suggesting that PO in L.vannamei is an oligomer,which may have different compositions intra-and extracellularly.     

Erratum to: Cloning, characterization, and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis)

<正>In the original article, the name of the fourth author is incorrect. The correct one is Kyoung Ho Kang, in which "Kang" is the family name. The publishing companies apologize to the author.     

Erratum to: Purification and characterization of iron-cofactored superoxide dismutase from Enteromorpha linza

<正>Erratum to:Chinese Journal of Oceanology and Limnology Vol.31 No.6,P.1190-1195,2013http://dx.doi.org/10.1007/s00343-013-3049-3The supporting grant information on the footnote of the original version of this article needs to be altered and updated.The new information is given below.     

Wind and sand control by an oasis protective system: a case from the southeastern edge of the Tengger Desert,China

Wind and sand control are important factors in combating desertification and protecting oases. An oasis protective system can provide these benefits, but quantitative research on protection effects has been lacking to date. This researchdescribes an oasis protective system in the southeastern border of the Tengger Desert. The system consists of a sand barrier belt, a shrub and herbaceous plant belt, and a farmland shelter belt.The system was compared to a bare dunes area as the control zone. The study investigated windproof effect,sediment transport, and erosion through fieldobservations. Results showed that the roughness of the shrub and herbaceous plant belt, farmland shelter belt, and sand barrier belt were increased compared with bare dunes. The shrub and herbaceous plant belt provided the highest windproof effect values for the same wind velocity measurement height, and the windproof effect values for different protective belts were as follows: shrub and herbaceous plant belt farmland shelter belt sand barrier belt. The sand barrier belt provided effective wind and sand control at heights from 0 to 50 cm above the ground. The total sediment transport for each protective belt is as follows: bare dunes sand barrier belt shrub and herbaceous plant belt farmland shelter belt. The sediment transport decreased exponentially as the height increased. In the bare dunes and protective systems, the sediment transport is mainly within 30 cm of the ground surface. The wind erosion intensity for this protective system was as follows: bare dunes sand barrier belt shrub and herbaceous plant belt farmland shelter belt. This research offers quantitative evidence for how oasis protective systems can effectively intercept sand and prevent erosion in oases. The results of this study can be applied in similar regions.