The specific molecular identification of life experiment (SMILE)

We describe a compact, highly integrated instrument concept for the detection and identification of a wide range of molecules associated with extinct/extant life or potential life processes. The Specific Molecular Identification of Life Experiment (SMILE) will be sensitive to the presence of a range of target molecules using both electrical and optical transduction techniques, and incorporates molecular imprinted polymers in addition to traditional biological receptors such as antibodies. A number of versions of the concept are possible depending on available resources e.g. mass, volume, etc. The full concept utilises a novel imaging interferometer where a large number of molecular receptors are deposited on the measurement plane of an imaging interferometer and read out by an imaging detector, enabling multiple targets—biomarkers—within a sample to be measured simultaneously. The optics can also form the basis of an UV-NIR imaging Fourier spectrometer allowing basic mineralogy studies to be conducted using optical properties to assist in the determination of the geological context of the samples. By incorporating micro-fabricated transducer arrays, micro-fluidics and artificial molecular recognition systems, as well as recombinant antibody technology with appropriate integration methods, SMILE forms a compact and robust “Life Marker Chip” which has been proposed for future planetary missions including ESA's ExoMars mission, where the instrument offers the possibility of conducting a direct in situ search for signs of past or present biological activity on Mars. In addition to its role in planetary exploration, derivatives of SMILE have multiple terrestrial applications in fields such as forensic analysis and environmental monitoring.     

Textural and mineralogical biosignatures in an unusual microbialite from Death Valley, California

For in situ astrobiological studies of Mars or other planets, we must employ strategies that will enable us to verify whether our approach and prototype instruments are actually capable of distinguishing life from non-life. This must be done against a background of rigorously conducted scientific characterization of the environment or sample types being considered for measurement by the instruments under development. In this study we show how a combination of mineralogical and textural features can be considered a biosignature in an early Mars analogue environment, Death Valley, California. We propose that it is a combination of features in context of the geologic matrix which allows determination of biogenicity to be made. Polymineralic microbialites (organosedimentary formations constructed by microorganisms) from a spring pool at Badwater, within Death Valley National Park, are composed of alternating biogenic and abiogenic minerals in a distinct triplet sequence related to wet and dry seasons. A microbial community, occurring as a black biofilm, produced paired layers of two different mineral types: manganese oxyhydroxides and calcite. These biogenic layers are separated from the next pair by a gypsum layer and appear to be laid down in the wet season, with the gypsum (a mineral positively identified on Mars) precipitating in the dry part of the year, abiogenically (i.e., not dependent on microbial metabolic activity for its deposition). In addition, textural features (smaller grain size and less geometric morphology) unique to the biogenic vs the abiogenic layers, were consistently observed so that texture served as a biosignature in this environment.     

Lunar Palaeoregolith Deposits as Recorders of the Galactic Environment of the Solar System and Implications for Astrobiology

One of the principal scientific reasons for wanting to resume in situ exploration of the lunar surface is to gain access to the record it contains of early Solar System history. Part of this record will pertain to the galactic environment of the Solar System, including variations in the cosmic ray flux, energetic galactic events (e.g., supernovae and/or gamma-ray bursts), and passages of the Solar System through dense interstellar clouds. Much of this record is of astrobiological interest as these processes may have affected the evolution of life on Earth, and perhaps other Solar System bodies. We argue that this galactic record, as for that of more local Solar System processes also of astrobiological interest, will be best preserved in ancient, buried regolith (‘palaeoregolith’) deposits in the lunar near sub-surface. Locating and sampling such deposits will be an important objective of future lunar exploration activities.     

The provenance,formation, and implications of reduced carbon phases in Martian meteorites

This review is intended to summarize the current observations of reduced carbon in Martian meteorites, differentiating between terrestrial contamination and carbon that is indigenous to Mars. Indeed, the identification of Martian organic matter is among the highest priority targets for robotic spacecraft missions in the next decade, including the Mars Science Laboratory and Mars 2020. Organic carbon compounds are essential building blocks of terrestrial life, so the occurrence and origin (biotic or abiotic) of organic compounds on Mars is of great significance; however, not all forms of reduced carbon are conducive to biological systems. This paper discusses the significance of reduced organic carbon (including methane) in Martian geological and astrobiological systems. Specifically, it summarizes current thinking on the nature, sources, and sinks of Martian organic carbon, a key component to Martian habitability. Based on this compilation, reduced organic carbon on Mars, including detections of methane in the Martian atmosphere, is best described through a combination of abiotic organic synthesis on Mars and infall of extraterrestrial carbonaceous material. Although conclusive signs of Martian life have yet to be revealed, we have developed a strategy for life detection on Mars that can be utilized in future life‐detection studies.     

Assessment of Raman spectroscopy as a tool for the non-destructive identification of organic minerals and biomolecules for Mars studies

Several characteristic geological features found on the surface of Mars by planetary rovers suggest that a possible extinct biosphere could exist based on similar sources of energy as occurred on Earth. For this reason, analytical instrumental protocols for the detection of biomarkers in suitable geological matrices unequivocally have to be elaborated for future unmanned explorations including the forthcoming ESA ExoMars mission. As part of the Pasteur suite of analytical instrumentation on ExoMars, the Raman/LIBS instrument will seek elemental and molecular information about geological, biological and biogeological markers in the Martian record. A key series of experiments on terrestrial Mars analogues, of which this paper addresses a particularly important series of compounds, is required to obtain the Raman spectra of key molecules and crystals, which are characteristic for each biomarker. Here, we present Raman spectra of several examples of organic compounds which have been recorded non-destructively—higher n-alkanes, polycyclic aromatic hydrocarbons, carotenoids, salts of organic acids, pure crystalline terpenes as well as oxygen-containing organic compounds. In addition, the lower limit of β-carotene detection in sulphate matrices using Raman microspectroscopy was estimated.     

Stereoscopic observations of solar flares made onboard the 2001 Mars Odyssey spacecraft and CORONAS-F satellite

The results of observations of solar hard radiation recorded by two spacecraft—2001 Mars Odyssey and CORONAS-F—which were located in the vicinity of Mars and Earth, respectively, are discussed. The HEND instrument, developed at the Space Research Institute of the Russian Academy of Sciences, recorded photons with energies ranging from 80 keV to 2 MeV, and the SPR and SONG instruments, developed at the Skobeltsyn Research Institute of Nuclear Physics of the Moscow State University, detected radiation in the energy interval from 15 keV to 100 MeV. The rising of the sunspot group 10486 in late October 2003, which had been observed from Martian orbit before it was seen from the Earth’s surface, is analyzed in detail. In this case, observations made from directions that differ by 24° showed a close-to-24 h advance for the detection of hard radiation of flares. Stereoscopic observations of M-class flares near the limb show that the overwhelming part of radiation with energies above 80 keV arises at heights that do not exceed 7–10 thousand km. Also reported are the results of observations of the powerful flare on August 25, 2001, by the two devices, which complement each other substantially. The processes resulting in the formation of high-energy radiation of solar flares are discussed.     

CH4/N2/H2 spark hydrophilic tholins: A systematic approach to the characterization of tholins

Tholins are complex organic materials produced by irradiation of several carbon and nitrogen rich atmospheres. They could have played a key role in the origin of life on Earth because their ability to release important bioorganics, which are nowadays present in proteins, nucleic-acid bases and other important biostructures. Usually, the yield of these compounds is higher after acid hydrolysis, however little is known about the structure and chemical composition of the tholins. In this work, we propose the use of different spectroscopic and separation techniques, which are not usually applied in this field, in order to obtain complete information about the tholin structure and behavior. Two different simulation experiments of prebiotic synthesis were carried out in CH₄/N₂/H₂ atmosphere out from spark discharge activation of aqueous aerosols and liquid water, respectively. In both cases, a hydrophilic tholin and a hydrophobic tholin were obtained. Herein, we report the application of this methodology to our hydrophilic tholins and we review, briefly, some astrobiological aspects related to these complex substances.     

Could we identify hot ocean-planets with CoRoT, Kepler and Doppler velocimetry?

Planets less massive than about 10 MEarth are expected to have no massive H-He atmosphere and a cometary composition (∼50% rocks, 50% water, by mass) provided they formed beyond the snowline of protoplanetary disks. Due to inward migration, such planets could be found at any distance between their formation site and the star. If migration stops within the habitable zone, this may produce a new kind of planets, called ocean-planets. Ocean-planets typically consist in a silicate core, surrounded by a thick ice mantle, itself covered by a 100 km-deep ocean. The possible existence of ocean-planets raises important astrobiological questions: Can life originate on such body, in the absence of continent and ocean-silicate interfaces? What would be the nature of the atmosphere and the geochemical cycles? In this work, we address the fate of hot ocean-planets produced when migration ends at a closer distance. In this case the liquid/gas interface can disappear, and the hot H₂O envelope is made of a supercritical fluid. Although we do not expect these bodies to harbor life, their detection and identification as water-rich planets would give us insight as to the abundance of hot and, by extrapolation, cool ocean-planets. The water reservoir of these planets seems to be weakly affected by gravitational escape, provided that they are located beyond some minimum distance, e.g. 0.04 AU for a 5-Earth-mass planet around a Sun-like star. The swelling of their water atmospheres by the high stellar flux is expected not to significantly increase the planets' radii. We have studied the possibility of detecting and characterizing these hot ocean-planets by measuring their mean densities using transit missions in space—CoRoT (CNES) and Kepler (NASA)—in combination with Doppler velocimetry from the ground—HARPS (ESO) and possible future instruments. We have determined the domain in the [stellar magnitude, orbital distance] plane where discrimination between ocean-planets and rocky planets is possible with these instruments. The brightest stars of the mission target lists and the planets closest to their stars are the most favorable cases. Full advantage of high precision photometry by CoRoT, and particularly Kepler, can be obtained only if a new generation of Doppler instruments is built.     

Higher-order neighbor analysis of the Tartarus Colles cone groups, Mars: The application of geographical indices to the understanding of cone pattern evolution

Cone fields of ambivalent origin exist in the Elysium, Amazonis, Cerberus, Isidis, Acidalia and Cydonia regions of Mars. Considerable interest exists in regard to their location and origin as their occurrence in many instances, is most likely associated with near surface ground ice; the presence of which has consequences for past and present climate, astrobiological activity, and future exploration. This research has outlined an understanding of both spatial and geomorphic processes of cone development for a region within the Tartarus Colles, Mars, as well as the manner in which spatial statistical indices can be affected by modification to the areal unit of study. This study found that cone groups for the region of the Tartarus Colles, are completely spatially random (CSR) across all distances extending to their sixth-order neighbor. However, significant statistical indices of aggregation were found for groups where post-emplacement erosional modification may have occurred by solifluction-like mechanisms or magmatic incursion into or over frozen ground. Such circumstances imply that R-indices reflect both the underlying spatial and geologic processes of cone formation, and also, an overprint of erosional modification of the cone-field within a glacial or periglacial setting, in this instance. The comparison of spatial indices can therefore offer a viewpoint from which geomorphic change can be recognized and quantitatively evaluated for cone fields.     

Prospects for Detecting the 326.5 MHz Redshifted 21-cm HI Signal with the Ooty Radio Telescope (ORT)

Observations of the redshifted 21-cm HI fluctuations promise to be an important probe of the post-reionization era (z≤ 6). In this paper we calculate the expected signal and foregrounds for the upgraded Ooty Radio Telescope (ORT) which operates at frequency ν _o = 326.5 MHz which corresponds to redshift z = 3.35. Assuming that the visibilities contain only the HI signal and system noise, we show that a 3 σ detection of the HI signal (～1 mK) is possible at angular scales 11^′ to 3^° with ≈1000 h of observation. Foreground removal is one of the major challenges for a statistical detection of the redshifted 21 cm HI signal. We assess the contribution of different foregrounds and find that the 326.5 MHz sky is dominated by the extragalactic point sources at the angular scales of our interest. The expected total foregrounds are 10⁴?10⁵ times higher than the HI signal.     

Thermal evolution of Pluto and implications for surface tectonics and a subsurface ocean

Determining whether or not Pluto possesses, or once possessed, a subsurface ocean is crucial to understanding its astrobiological potential. In this study we use a 3D convection model to investigate Pluto’s thermal and spin evolution, and the present-day observational consequences of different evolutionary pathways. We test the sensitivity of our model results to different initial temperature profiles, initial spin periods, silicate potassium concentrations and ice reference viscosities. The ice reference viscosity is the primary factor controlling whether or not an ocean develops and whether that ocean survives to the present day. In most of our models present-day Pluto consists of a convective ice shell without an ocean. However if the reference viscosity is higher than 5 × 10¹⁵ Pa s, the shell will be conductive and an ocean should be present. For the nominal potassium concentration the present-day ocean and conductive shell thickness are both about 165 km; in conductive cases an ocean will be present unless the potassium content of the silicate mantle is less than 10% of its nominal value. If Pluto never developed an ocean, predominantly extensional surface tectonics should result, and a fossil rotational bulge will be present. For the cases which possess, or once possessed, an ocean, no fossil bulge should exist. A present-day ocean implies that compressional surface stresses should dominate, perhaps with minor recent extension. An ocean that formed and then re-froze should result in a roughly equal balance between (older) compressional and (younger) extensional features. These predictions may be tested by the New Horizons mission.     

Clues on the importance of comets in the origin and evolution of the atmospheres of Titan and Earth

Earth and Titan are two planetary bodies formed far from each other. Nevertheless the chemical composition of their atmospheres exhibits common indications of being produced by the accretion, plus ulterior in-situ processing of cometary materials. This is remarkable because while the Earth formed in the inner part of the disk, presumably from the accretion of rocky planetesimals depleted in oxygen and exhibiting a chemical similitude with enstatite chondrites, Titan formed within Saturn's sub-nebula from oxygen- and volatile-rich bodies, called cometesimals. From a cosmochemical and astrobiological perspective, the study of the H, C, N, and O isotopes on Earth and Titan could be the key to decipher the processes occurred in the early stages of formation of both planetary bodies. The main goal of this paper is to quantify the presumable ways of chemical evolution of both planetary bodies, in particular the abundance of CO and N₂ in their early atmospheres. In order to do that the primeval atmospheres and evolution of Titan and Earth have been analyzed from a thermodynamic point of view. The most relevant chemical reactions involving these species and presumably important at their early stages are discussed. Then, we have interpreted the results of this study in light of the results obtained by the Cassini–Huygens mission on these species and their isotopes. Given that H, C, N, and O were preferentially depleted from inner disk materials that formed our planet, the observed similitude of their isotopic fractionation, and subsequent close evolution of Earth's and Titan's atmospheres points towards a cometary origin of Earth atmosphere. Consequently, our scenario also supports the key role of late veneers (comets and water-rich carbonaceous asteroids) enriching the volatile content of the Earth at the time of the late heavy bombardment of terrestrial planets.     

Lunar Beagle and Lunar Astrobiology

The study of the elements and molecules of astrobiological interest on the Moon can be made with the Gas Analysis Package (GAP) and associated instruments developed for the Beagle 2 Mars Express Payload. The permanently shadowed polar regions of the Moon may offer a unique location for the “cold-trapping” of the light elements (i.e. H, C, N, O, etc.) and their simple compounds. Studies of the returned lunar samples have shown that lunar materials have undergone irradiation with the solar wind and adsorb volatiles from possible cometary and micrometeoroid impacts. The Beagle 2’s analytical instrument package including the sample processing facility and the GAP mass spectrometer can provide vital isotopic information that can distinguish whether the lunar volatiles are indigenous to the moon, solar wind derived, cometary in origin or from meteoroids impacting on the Moon. As future Lunar Landers are being considered, the suite of instruments developed for the Mars Beagle 2 lander can be consider as the baseline for any lunar volatile or resource instrument package.     

The Argentinean Patagonia and the Martian landscape

Throughout the Cenozoic Era, the geological history of the Argentinean Patagonia was dominated by basaltic volcanism and glacial and periglacial environments. Several geological and geomorphological processes that concurred to the sculpting of the landscape of this area could have been similar to those responsible of the shaping of the Martian surface. In this work a survey of some high-resolution satellite images of the Argentinean Patagonia is performed in order to identify possible geomorphological analogs of the Martian surface. Several morphologies that resemble Martian features are presented and discussed. They consist of proglacial and periglacial features, relatively small circular depressions, gullies, fan-deltas, eolian streaks, and diluvial dunes. Results suggest that the Argentinean Patagonia appears to consist of an interesting terrestrial analog for the Martian landscape. Furthermore, the study area shows to be interesting in order to test robotic instruments and human missions equipment, to train astronauts of future human expeditions to Mars, and to perform astrobiological experiments.     

Overview of analogue science activities at the McGill Arctic Research Station,Axel Heiberg Island,Canadian High Arctic

The Canadian High Arctic contains several of the highest fidelity Mars analogue sites in the world. Situated at nearly 80° north, Expedition Fjord on Axel Heiberg Island is located within a polar desert climate, with the surrounding landscape and conditions providing an invaluable opportunity to examine terrestrial processes in a cold, dry environment. Through the Canadian Space Agency's Analogue Research Network program, scientific activities based out of the McGill Arctic Research Station (M.A.R.S.) are extremely broad in scope, representing physical, biological, and technological investigations. Some of the most unique hydrogeologic features under investigation near M.A.R.S. are a series of cold saline springs that maintain liquid-state flow year round regardless of air temperature. Previous studies have examined their geomorphic relation to discharge-related formations, water chemistry, temperature monitoring, discharge rates, and combined flow/thermal modeling. Recent investigations have identified microbial communities and characterized biological activity within the springs and within permafrost sections, having direct relevance to astrobiological analogue research goals. Another main thrust of research activities based at M.A.R.S. pertains to the detection, mapping, and quantification of subsurface ice deposits. A long-term study is presently underway examining polygonal terrain, comparing surficial patterns found in the region with those identified on Mars, and using surface morphology to estimate ice wedge volumes through a combination of aerial photography interpretation and ground-based geophysical techniques. Other technological developments include the use of in situ microscopy for the detection of biomarkers and improved permafrost drilling techniques. This paper presents an overview of previous studies undertaken at M.A.R.S. over the past decades and will describe in detail both present and upcoming work.     

Heavy ion formation in Titan's ionosphere: Magnetospheric introduction of free oxygen and a source of Titan's aerosols?

Discovery by Cassini's plasma instrument of heavy positive and negative ions within Titan's upper atmosphere and ionosphere has advanced our understanding of ion neutral chemistry within Titan's upper atmosphere, primarily composed of molecular nitrogen, with ~2.5% methane. The external energy flux transforms Titan's upper atmosphere and ionosphere into a medium rich in complex hydrocarbons, nitriles and haze particles extending from the surface to 1200 km altitudes. The energy sources are solar UV, solar X-rays, Saturn's magnetospheric ions and electrons, solar wind and shocked magnetosheath ions and electrons, galactic cosmic rays (GCR) and the ablation of incident meteoritic dust from Enceladus’ E-ring and interplanetary medium. Here it is proposed that the heavy atmospheric ions detected in situ by Cassini for heights >950 km, are the likely seed particles for aerosols detected by the Huygens probe for altitudes <100 km. These seed particles may be in the form of polycyclic aromatic hydrocarbons (PAH) containing both carbon and hydrogen atoms C_nH_x. There could also be hollow shells of carbon atoms, such as C₆₀, called fullerenes which contain no hydrogen. The fullerenes may compose a significant fraction of the seed particles with PAHs contributing the rest. As shown by Cassini, the upper atmosphere is bombarded by magnetospheric plasma composed of protons, H₂⁺ and water group ions. The latter provide keV oxygen, hydroxyl and water ions to Titan's upper atmosphere and can become trapped within the fullerene molecules and ions. Pickup keV N₂⁺, N⁺ and CH₄⁺ can also be implanted inside of fullerenes. Attachment of oxygen ions to PAH molecules is uncertain, but following thermalization O+ can interact with abundant CH₄ contributing to the CO and CO₂ observed in Titan's atmosphere. If an exogenic keV O+ ion is implanted into the haze particles, it could become free oxygen within those aerosols that eventually fall onto Titan's surface. The process of freeing oxygen within aerosols could be driven by cosmic ray interactions with aerosols at all heights. This process could drive pre-biotic chemistry within the descending aerosols. Cosmic ray interactions with grains at the surface, including water frost depositing on grains from cryovolcanism, would further add to abundance of trapped free oxygen. Pre-biotic chemistry could arise within surface microcosms of the composite organic-ice grains, in part driven by free oxygen in the presence of organics and any heat sources, thereby raising the astrobiological potential for microscopic equivalents of Darwin's “warm ponds” on Titan.     

CH4/N2/H2-spark hydrophobic tholins: A systematic approach to the characterisation of tholins. Part II

Two different simulation experiments of prebiotic synthesis were carried out in a CH₄/N₂/H₂ atmosphere with spark discharge activation of aqueous aerosols and liquid water. In both cases, a hydrophilic tholin and a hydrophobic tholin were obtained. The methodology developed by our group for the characterisation of hydrophilic tholins [Ruiz-Bermejo, M., Menor-Salván, C., Mateo-Martí, E., Osuna-Esteban, S., Martín-Gago, J.A., Veintemillas-Verdaguer, S., 2008. Icarus 198, 232-241] was used in order to study the hydrophobic tholins. The gas precursors of the tholins from mixtures containing CH₄, with and without H₂, were studied. We propose that the formation of the hydrophobic tholins involves the formation of unsaturated oligomeric hydrocarbon chains from vinyl and acetylene monomers, as well as allene derivatives formed in the gas phase after the incorporation of polar groups into these hydrocarbon chains. Finally, we compare our results concerning hydrophobic tholins with HCN polymers, since it is generally suggested that the polymeric material formed in spark experiments are possible oligomers of HCN, and that Titan’s tholins could be poly-HCN.     

Instrument development to search for biomarkers on mars: Terrestrial acidophile, iron-powered chemolithoautotrophic communities as model systems

Recent findings by the MER rover opportunity confirming the presence of iron minerals that can only be formed in the presence of water emphasize the study of analogous environments to Mars on Earth. The study of chemolithoautotrophic communities living in acidic iron-rich habitats is highly relevant in order to identify Mars analog environment-specific biomarkers. Iron oxidizing bacteria like Leptospirillum ferrooxidans and Acidithiobacillus ferrooxidans have ways of life for which it is feasible to identify a past or present hypothetical niche on Mars. We have developed a strategy for biomarker identification based on: (i) search for biosignatures on acid and metal-rich environments; (ii) development of an immunosensor microarray; and (iii) integration into an instrument for autonomous and remote operation. The instrument that we have built, called Signs Of LIfe Detector (SOLID), is capable of processing a variety of samples for the detection of specific biomarkers. Antibodies against several bacterial strains have been developed and tested in a microarray biosensor on SOLID. Tests with field samples have been successfully performed, allowing the detection of L. ferrooxidans, A. ferrooxidans present in sediment samples.     

Do ice caves exist on Mars?

We have developed a numerical model for assessing the lifetime of ice deposits in martian caves that are open to the atmosphere. Our model results and sensitivity tests indicate that cave ice would be stable over significant portions of the surface of Mars. Ice caves on Earth commonly occur in lava tubes, and Mars has been significantly resurfaced by volcanic activity during its history, including the two main volcanic provinces, the Tharsis and Elysium rises. These areas, known or suspected of having subsurface caves and related voids are among the most favorable regions for the occurrence of ice stability. The martian ice cave model predicts regions which, if caves occur, would potentially be areas of astrobiological importance as well as possible water sources for future human missions to Mars.     

Top layers charaterization of the Martian surface: Permittivity estimation based on geomorphology analysis

Mars Express spacecraft inserted successfully Martian orbit at the end of 2003. On board this probe, a radar instrument called MARSIS (for Mars Advanced Radar for Surface and Ionosphere Sounding) is looking for water inside the first kilometers of Martian crust. To support MARSIS planning and data inversion, Laboratoire de Planétologie de Grenoble developed a MARSIS signal simulator.We show in this paper that MARSIS can also characterize some surface features, in addition to subsurface water and ionosphere sounding. We study a Martian surface region of special interest: Nilokeras Mensae, inside Acidalia Planitia. We discuss the previous geological studies of this region, and show the geomorphologies analyze of this surface area could lead to a simple terrain model. Then, we present a possible data inversion scheme and applying the MARSIS simulator, we test a first radar data inversion.Finally, we will show that complete dielectric characteristics of surface top layers can be retrieved, at least as often Mars Express flies over some layered terrain (at wavelength scale).