Sequencing and expression analysis of CD3γ/δ and CD3εchains in mandarin fish, Siniperca chuatsi

The genomic and cDNA sequences of the CD3γ/δ and CD3ε homologues in the mandarin fish, Siniperca chuatsi, were determined. As in other vertebrate CD3 molecules, the deduced amino acid sequences of mandarin fish CD3γ/δ and CD3ε contained conserved residues and motifs, such as cysteine residues and CXXC and immunoreceptor tyrosine-based activation motifs. However, mandarin fish CD3γ/δ and CD3ε showed some differences to their mammalian counterparts, specifically the absence of a negatively charged residue in the transmembrane region of CD3γ/δ. Additionally, while an N-glycosylation site was present in CD3ε, the site was not observed in CD3γ/δ. The CD3γ/δ and CD3ε subunit sequences contain six and five exons, respectively, consistent with homologues from Atlantic salmon, Salmo salar. Phylogenetic analysis also revealed that CD3γ/δ and CD3ε in mandarin fish are closely related to their counterparts in Acanthopterygian fish. Real-time PCR showed CD3γ/δ and CD3ε were expressed mainly in the thymus and spleen in normal healthy fish and, to a lesser extent, in mucosal-associated lymphoid tissues, such as the intestine and gills. When lymphocytes isolated from head kidney were treated with the mitogens phytohemagglutinin, concanavalin, and polyriboinosinic polyribocytidylic acid, mRNA expression levels of CD3γ/δ and CD3ε were significantly elevated within 12 h of treatment. This indicated the presence of T lymphocytes in the head kidney of teleost fish, and also the recognition of mitogens by the lymphocytes. Mandarin fish infected with the bacterial pathogen Flavobacterium columnare also showed an increase in the expression of CD3γ/δ and CD3ε mRNA, indicating that CD3γ/δ and CD3ε lymphocytes are involved in the immune response of this species.     

一种基于FY3D/MERSI2的AOD遥感反演方法

针对目前国产卫星对气溶胶遥感监测应用较少的情况,本研究综合暗象元和SARA算法优势,基于FY-3D MERSI2数据构建了一套气溶胶光学厚度(Aerial Optical Depth, AOD)快速遥感反演方法。首先,引入了传统暗象元算法中的地表反射率经验关系,然后利用AERONET长时间序列的地基观测数据,构建了红波段和蓝波段气溶胶光学厚度、不对称因子和单次散射率之间的关系,最后利用MOD04和AERONET的AOD产品对研究反演结果进行了验证和评估分析。结果发现：① 本研究反演的AOD不仅保持了与MODIS的气溶胶产品空间分布的一致性,而且合理地呈现了AOD的高值分布,改进了MOD04气溶胶产品在云和亮目标方面反演缺失问题;② 利用AERONET地基观测结果对本研究获取的MERSI2的AOD反演结果进行了对比分析,发现二者具有较高的线性相关性,蓝波段AOD线性相关系数超过0.85;③ 利用MERSI2数据完整地捕捉到了2018年3月9—14日京津冀及周边区域的一次重污染过程中气溶胶时空分布变化情况,这也说明了FY3D卫星具备良好的气溶胶遥感监测能力,为我国灰霾监测和预警提供参考依据。本研究对大力发展国产卫星在大气环境遥感的应用有重要参考意义。     

f-3项与投影函数对电离层延迟的影响

依据Chapman正常电离层理论,构造Chapman剖面函数加权投影函数,给出VTEC三角多项式模型,并以武汉站2005年1月1日观测数据进行实例解算,分析电离层延迟f-3项、电离层单层高度、电离层投影函数形式等对电离层延迟的影响.     

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