钙网蛋白相关信号通路在肿瘤中的研究进展

钙网蛋白（calreticulin,CRT）是参与多种细胞过程的高度保守的内质网伴侣蛋白。1974年,它首次被鉴定为钙离子结合蛋白,随后CRT与肿瘤之间信号通路相关关系被广泛的研究。另外,在人类多种肿瘤中,细胞表面的CRT被认为是1个"促吞噬"信号,由此CRT与癌细胞的巨噬细胞吞噬免疫系统的相关研究,有望为肿瘤综合治疗提供新的理论依据和策略。也有越来越多的证据表明CRT在不同肿瘤中的异常表达,将为CRT成为一种新的肿瘤标志物提供许多依据。以及CRT对各种肿瘤的发展具有很重要的影响且CRT对肿瘤形成和进展的影响取决于细胞类型和临床阶段。     

THERMALLY DRIVEN SORPTION,DESORPTION, AND MOISTURE MIGRATION IN THE ACTIVE LAYER IN CENTRAL ALASKA

Combined observations of hourly soil temperature and electric potential, the latter converted to a relative index of soil-water solute concentration, yield information on the physical chemistry of near-surface frost effects. Solute concentration near the descending 0° C isotherm in the refreezing active layer above permafrost is divided into three distinct zones: (1) an ion-enriched zone in the unfrozen active layer that precedes the penetrating freezing front; (2) an ion-purified desorbed zone at the freezing front that is the source region of the downward-expelled ions and water; and (3) a hydrologically isolated subfreezing zone of enhanced solute concentration located above the freezing isotherm. High-frequency fluctuations superimposed on these general patterns are traceable to vapor migration driven by surface thermal fluctuations. These effects diminish at temperatures below about -0.4° C, as permeability decreases with soil-ice formation. The combined temperature-solute concentration time series is used to develop sorption curves for the frozen organic and mineral soils, and indicates that approximately half of the pore water present in the mineral soil at -0.4° C had not been converted to ice at -6° C. Gradual soil desiccation over winter appears to result from outward vapor diffusion, possibly through soil cracks. [Key words: Alaska, active layer, frozen ground, soil temperature, soil water, permafrost.]     

1981—2018年内蒙古典型草原季节性冻土对气候变化的响应

以1981—2018年内蒙古典型草原季节性冻土为研究对象,通过气候倾向率、Mann-Kendall法、多元线性回归等方法,分析最大冻土时空分布特征、年际、年代际变化,研究影响最大冻土深度变化的气象因子。结果表明：（1） 内蒙古典型草原季节性冻土冻结初日在9—11月,终日在4—6月,年内最大冻土深度出现在2—3月,深度在100~280 cm之间。（2） 最大冻土深度年际变化分为下开口抛物线型、上开口抛物线型、正弦曲线型,从最大冻土深度气候倾向率看呈现减小趋势的站点有68%。（3） 最大冻土深度年代际变化分为逐年代递减、减-增型和无明显变化规律,50%的站点在1989年以后最大冻土深度发生突变。（4） 多元线性回归表明气温冻结指数、年平均风速、年极端最低气温对最大冻土深度产生显著影响。该研究揭示了最大冻土深度存在退化的事实,为草原应对气候变化提供指导,为陆地土壤和大气碳循环交换的研究给出提示。     

Mechanisms discussion of frost heave and test verifications of moisture migration

Herein we review research on the structure of the frozen fringe and one of its key characteristics（unfrozen water content）,and compare its current measurement methods,including pulsed nuclear magnetic resonance（NMR）,time-domain reflectometry（TDR）,calorimetry,thermometry,the sublimation method,and CT imagery.A freeze-thaw cycle experiment with remolded soil was conducted inside to verify the mechanism of frost heave,measuring such variables as soil-water potential,temperature,water supplement,and the position of the freezing front.Conclusions from the analysis of the experiment data are：（1） The soil-water potential,the water supplement,and the position of the freezing front vary with temperature; and（2） the temperature gradient induces the soil-water potential,which in turn provides a stable driving force for moisture migration.     

Revision of thickness design of cylindrical frozen walls considering frost heave

This paper outlines development of the thickness design of cylindrical frozen walls in artificial ground freezing (AFG). A plain strain mechanical model coupled with infinite surrounding soil and rock ...     

Influence of repeated freeze-thaw on dynamic modulus and damping ratio properties of silty sand

Under repeated freezing and thawing in deep seasonal frozen regions, the stability and strength of the soil are imposed in the form of large uneven settlement, instability and strength reduction, which...     

Influence of fines content on the anti-frost properties of coarse-grained soil

This paper aims to determine the optimal fines content of coarse-grained soil required to simultaneously achieve weaker frost susceptibility and better bearing capacity. We studied the frost susceptibility and strength properties of coarse-grained soil by means of frost heaving tests and static triaxial tests, and the results are as follows:(1) the freezing temperature of coarse-grained soil decreased gradually and then leveled off with incremental increases in the percent content of fines; (2) the fines content proved to be an important factor influencing the frost heave susceptibility and strength properties of coarse-grained soil. With incremental increases in the percent content of fines, the frost heave ratio increased gradually and the cohesion function of fines effectively enhanced the shear strength of coarse-grained soil before freeze-thaw, but the frost susceptibility of fines weakened the shear strength of coarse-grained soil after freeze-thaw; (3) with increasing numbers of freeze-thaw cycles,the shear strength of coarse-grained soil decreased and then stabilized after the ninth freeze-thaw cycle, and therefore the mechanical indexes of the ninth freeze-thaw cycle are recommended for the engineering design values; and (4) considering frost susceptibility and strength properties as a whole, the optimal fines content of 5% is recommended for railway subgrade coarse-grained soil fillings in frozen regions.     

Experimental study on moisture migration of remodeled clay under different overburden pressure and temperature gradients

Unidirectional freezing experiments under overburden pressure were carried out, in order to study the driving force of mois-ture migration of remodeled clay during freezing, through improving the indoo...     

Model test study of frost heaving pressures in tunnels excavated in fractured rock mass in cold regions

Based on the similarity theory, taking the horseshoe, city-gate and round linings as examples, the value and distribution regularities of normal frost heaving pressures （hereinafter as frost heaving pressures） in tunnels excavated in fractured rock mass in cold regions under different constraints and freezing depths were studied by a test model. It was found that the larger the frozen depth, the larger the frost heaving pressure, and the stronger the top constraint, the larger the frost heaving pressure. For the horseshoe lining and city-gate lining, the top constraint has a greater effect on the frost heaving pressures on the arch and the inverted arch. For the round lining, the influences of the top constraint on the frost heaving pressure in all linings are almost the same. The frost heaving pressure is maximum on the city-gate lining and minimal on the round lining. The largest frost heaving pressure all occur near the foot of the inverted arch for the three kinds of lining. Thus, the test data basically coincide with the observed in situ data.     

Seasonally frozen soil effects on the dynamic behavior of highway bridges

Frozen ground is significantly stiffer than unfrozen ground. For bridges supported on deep foundations, bridge stiffness is also measurably higher in winter months. Significant changes due to seasonal freezing in bridge pier boundary conditions require additional detailing in order to ensure a ductile performance of the bridge during a design earthquake event. This paper reports the latest results obtained from a project that systematically investigated the effects of seasonally frozen soil on the seismic behavior of highway bridges in cold regions. A bridge was chosen and was monitored to study its seismic performance and assess the impact of seasonally frozen soil on its dynamic properties. A Finite Element (FE) model was created for this bridge to analyze the impact of seasonal frost. It was found that when frost depth reaches 1.2 m, the first transverse modal frequency increases about 200% when compared with the no-frost case. The results show that seasonal frost has a significant impact on the overall dynamic behavior of bridges supported by pile foundations in cold regions, and that these effects should be accounted for in seismic design.     

低干密度盐渍细砂土低温特性试验研究

利用2015年9月采自青海北霍布逊湖区的原土样,自行配比进行了室内盐渍细砂土冻胀试验,研究了低干密度盐渍细砂土的低温特性变化规律,探讨了盐渍细砂土的冻胀机理,集中分析了土的冻缩特性。研究发现低干密度盐渍细砂土在不同含水率条件下土的冻胀规律差异很大。低含水率条件下会有冻缩现象,高含水率条件下只会有冻胀现象,临界含水率（含水率14%）条件下细砂土先出现冻缩,之后随着温度的降低又出现冻胀,且由于温度引起的体积变化均在-28℃～-30℃时达到稳定;土的冻缩随着含盐量增加先呈递增趋势,后又呈递减趋势,在中间含盐量10%达到最大;除临界含水率条件下,盐渍细砂土的冻胀、冻缩均与温度呈三次多项式关系,与含水率呈线性关系。研究结果能够为盐渍土地区的工程建设提供有价值的理论依据。     

Artificially frozen ground and related engineering technology in Japan

Since the 1970's, frozen ground has been developing near the Tokyo Bay area around liquefied natural gas(LNG) inground storage tanks. For disaster prevention purposes, the tanks are constructed below the ground surface. Since the temperature of the liquid stored in the tanks is -162℃ the soil surrounding the tanks freezes. Since this frozen ground has existed for almost half a century, we have permafrost near Tokyo. The development of artificial frozen ground may cause frost heaving, resulting in frost heave forces that may cause structural damage of adjacent LNG in-ground storage tanks.Therefore, the demand for frozen ground engineering increased and consequently we now have advanced technology in this area. Fortunately, we use this engineering technology and artificial ground freezing for civil engineering, especially in big and crowded cities like Tokyo. This paper provides a summary of the testing apparatus, test methods, and assessment methods for frost heaving.     

Freezing and thawing effects of sand-silt mixtures on elastic waves

Freezing and thawing during the winter season change soil properties such as density. The density change in the particulate media influences soil stiffness. In addition, freezing of partially or fully saturated soils changes the soil matrix from a particulate media to a continuum. The goal of this study is to investigate the cyclic freezing and thawing effects on elastic waves. Sand-silt mixtures with 10% silt fraction in weight and 40% saturation are prepared. The sand-silt mixtures are placed in a nylon cell, onto which a pair of bender elements and a pair of piezoelectric disk elements are installed for the measurement of shear and compressional waves, respectively. The temperature of the mixtures decreases from 20°C to 10°C to freezing. The frozen sample is gradually thawed at room temperature (20°C). These freezing-thawing processes are repeated three times. The test result shows that the shear and compressional wave velocities significantly increase when the specimen is frozen. When the temperature is greater than 0°C, the elastic wave velocities are lower during thawing than during freezing due to soil structure change. This study demonstrates that soil structure change during the winter season may be effectively estimated from elastic waves.     

Moisture transfer and phase change in unsaturated soils: an experimental study of two types of canopy effect

Canopy effect refers to the phenomenon in which moisture accumulates underneath an impervious cover. A canopy effect can lead to full saturation of the soil underneath the impervious cover. A recent theoretical study separates the canopy effect into two types. The first one is caused by evaporation-condensation in unsaturated soils, while the second one is induced by freezing-enhanced vapour transfer in unsaturated soils. To validate experimentally these two types of canopy effect and to reveal their mechanisms, moisture-migration experiments were carried out, using a newly developed laboratory apparatus for unsaturated frozen soils. Six conditions were applied to the calcareous sand, with different initial water contents and boundary temperatures. The results show that water content in the upper portion of the sample increased under an upward temperature gradient, and the increment of water content was greater if the soil was subjected to freezing. For the freezing cases, the depth of the peak water content was in line with the freezing front. And the greater the initial water content, the more the water content accumulated at the freezing front. However, a lower cooling rate seemed to facilitate vapour migration. For the unfrozen cases, the water content in the upper portion of the sample also increased; and the increases became more apparent with a higher initial moisture content. The temperature gradient can also inhibit the vapour migration. A less steep temperature gradient always resulted in a more notable inhibition effect. Test results seem to verify the theory of the canopy effect.     

Experimental investigations of freezing soils at ground conditions of Astana, Kazakhstan

Kazakhstan regions is seasonal climatic with transient freezing of soil groundduring the winter. Roadbed integrity is important to resist the sustained load transmitted by traffic on the road surface. Freezing of soil ground could significantlyinfluence roadbed integrity in the seasonal freezing climate of Kazakhstan. The proper determination magnitude of frost heave and heaving pressure by the influence of freezing temperatures during the winter season are necessary for design and construction of highways. Thus, experimental tests were conducted on specimens obtained from Astana (Kazakhstan) to determine the freezing pressure and magnitude of frost heaving.     

SPECTRAL SIGNATURE OF COUPLED FLOW IN THE REFREEZING ACTIVE LAYER,NORTHERN ALASKA

Soil temperature records obtained from the active layer above permafrost at a site in northern Alaska during autumn and winter have variance spectra inconsistent with a purely conductive heat-transfer system. Although conductive heat-transfer theory predicts that temperature fluctuations are attenuated with depth, sub-diurnal thermal variance at the 50-cm level, near the base of the active layer, exceeded that at the 10-cm level. Short segments of the temperature record were drawn from three distinct periods of soil-frost conditions: (1) at the maximum vertical development of the active layer in early autumn; (2) during frost penetration and the formation of a zero curtain in early winter; and (3) after freezeback of the active layer. The variance spectra of these time series show systematic seasonal transitions that reflect changing mechanisms of heat transfer. During the first and second periods, heat transfer by internal evaporation and condensation dominates at wavelengths in the diurnal range. The spectral traces are not strongly self-similar and the fractal dimensions indicate extreme space-filling, especially at deeper levels. Once the active layer is frozen, conductive heat transfer dominates, producing a trend toward self-similarity. Both the thermal variance and the fractal dimension decrease with depth in the frozen regime. [Key words: Alaska, active layer, coupled flow, fractal dimension, frozen ground, heat transfer, permafrost, soil freezing, spectral analysis, zero curtain.]     

Different discretization method used in coupled water and heat transport mode for soil under freezing conditions

A coupled water and heat transport mode is established based on the Richards equation to study water flow and heat transport in soil during freezing process. Both the finite difference and finite element method are used in the discretization, respectively. Two different computer programs are written and used to simulate an indoor unidirectional frozen test. The freezing depth, freezing rate and temperature variation are compared among lab tests, finite difference calculation simulation and finite element calculation simulation. Result shows that: the finite difference method has a better performance in freezing depth simulation while the finite element method has a better performance in numerical stability in one-dimensional freezing simulation.     

毛乌素沙地砒砂岩与沙复配成土技术固沙效应

为研究砒砂岩与沙复配成土技术的固沙效应,本研究采用试验小区与大田试验相结合的方法,监测了土壤结皮厚度、土壤冻层深度、土壤水分、地表粗糙度和积雪消融等表征固沙效果的相关指标。结果表明:随着砒砂岩的引入,复配土中黏粉粒含量增加,土壤饱和导水率降低,而土壤含水量、结皮厚度、地表粗糙度和冻层深度都有所增加,复配土抗风蚀能力增强。在冬季,通过发展保护性耕作,农作物残茬、根系以及土地整治之后地形因素的改变等对防止土壤风蚀也起到了积极的作用。该研究可为砒砂岩与沙复配成土造田技术的固沙效应提供更充分的理论依据。     

Study of experiment method for triaxial frost heaving constitutive relation of frozen soil

A new experiment method is introduced to study the relations between frost heaving strain and stress of soil or fracture rock under different moisture contents, temperatures and stress states. Based on experiments, a new triaxial frost heaving stress-strain relation is presented aiming at tunnel in cold regions. The experimental equipments are strain-controlled and low temperature buildup, and different restrained conditions are controlled by changing the stiffness of a test-force-ring. Then the frost heaving strain and stress of soil or fracture rock can be obtained under different restrained conditions, and the frost heaving stress-strain relation can be got by regression analysis of some feature points. Experiments of saturated sandy soil conducted by this method show that the triaxial frost heaving stress-strain relation could be expressed by logarithmic curves, and the frost heaving stress changes linearly with the logarithm of the frost heaving strain. The stronger the constraint, the smaller the frost heaving strain and the larger the frost heaving stresses. The frost heaving stresses would tend to a limit value with increasing the constraint intensity. The larger the confining pressure, the larger the frost heaving strain and stress.     

Test on dynamic characteristics of subgrade of heavy-haul railway in cold regions

Dynamic characteristics of heavy-haul railway subgrade under vibratory loading in cold regions are investigated via low-temperature dynamic triaxial tests with multi-stage cyclic loading process. The relationship between dynamic shear stress and dynamic shear strain of frozen soil of subgrade under train loading and the influence of freezing temperatures on dynamic constitutive relation, dynamic shear modulus and damping ratio are observed in this study. Test results show that the dynamic constitutive relations of the frozen soils with different freezing temperatures comply with the hyperbolic model, in which model parameters a and b decrease with increasing freezing temperature. The dynamic shear modulus of the frozen soils decreases with increasing dynamic shear strains initially, followed by a relatively smooth attenuation tendency, whereas increases with decreasing freezing temperatures. The damping ratios decrease with decreasing freezing temperatures. Two linear functions are defined to express the linear relationships between dynamic shear modulus (damping ratio) and freezing temperature, respectively, in which corresponding linear coefficients are obtained through multiple regression analysis of test data.