Integrating environmental variables and WorldView-2 image data to improve the prediction and mapping of Thaumastocoris peregrinus (bronze bug) damage in plantation forests

This study integrated environmental variables together with high spectral resolution WorldView-2 imagery to detect and map Thaumastocoris peregrinus damage in Eucalypt plantation forests in KwaZulu-Natal, South Africa. The WorldView-2 bands, vegetation indices and environmental variables were entered separately into PLS regression models to predict T. peregrinus damage. The datasets were then integrated to test the collective strength in predicting T. peregrinus damage. Important variables were identified by variable importance (VIP) scores and were re-entered into a PLS regression model. The VIP model was then extrapolated to map the severity of damage and predicted T. peregrinus damage with an R² value of 0.71 and a RMSE of 3.26% on an independent test dataset. The red edge and near-infrared bands of the WorldView-2 sensor together with the temperature dataset were identified as important variables in predicting T. peregrinus damage. The results indicate the potential of integrating WorldView-2 data and environmental variables to improve the mapping and monitoring of insect outbreaks in plantation forests. The result is critical for plantation health monitoring using a new sensor which contains important vegetation wavelengths.     

Non-linear partial least square regression increases the estimation accuracy of grass nitrogen and phosphorus using in situ hyperspectral and environmental data

Grass nitrogen (N) and phosphorus (P) concentrations are direct indicators of rangeland quality and provide imperative information for sound management of wildlife and livestock. It is challenging to estimate grass N and P concentrations using remote sensing in the savanna ecosystems. These areas are diverse and heterogeneous in soil and plant moisture, soil nutrients, grazing pressures, and human activities. The objective of the study is to test the performance of non-linear partial least squares regression (PLSR) for predicting grass N and P concentrations through integrating in situ hyperspectral remote sensing and environmental variables (climatic, edaphic and topographic). Data were collected along a land use gradient in the greater Kruger National Park region. The data consisted of: (i) in situ-measured hyperspectral spectra, (ii) environmental variables and measured grass N and P concentrations. The hyperspectral variables included published starch, N and protein spectral absorption features, red edge position, narrow-band indices such as simple ratio (SR) and normalized difference vegetation index (NDVI). The results of the non-linear PLSR were compared to those of conventional linear PLSR. Using non-linear PLSR, integrating in situ hyperspectral and environmental variables yielded the highest grass N and P estimation accuracy (R² = 0.81, root mean square error (RMSE) = 0.08, and R² = 0.80, RMSE = 0.03, respectively) as compared to using remote sensing variables only, and conventional PLSR. The study demonstrates the importance of an integrated modeling approach for estimating grass quality which is a crucial effort towards effective management and planning of protected and communal savanna ecosystems.     

Estimating salinity stress in sugarcane fields with spaceborne hyperspectral vegetation indices

The presence of salt in the soil profile negatively affects the growth and development of vegetation. As a result, the spectral reflectance of vegetation canopies varies for different salinity levels. This research was conducted to (1) investigate the capability of satellite-based hyperspectral vegetation indices (VIs) for estimating soil salinity in agricultural fields, (2) evaluate the performance of 21 existing VIs and (3) develop new VIs based on a combination of wavelengths sensitive for multiple stresses and find the best one for estimating soil salinity. For this purpose a Hyperion image of September 2, 2010, and data on soil salinity at 108 locations in sugarcane (Saccharum officina L.) fields were used. Results show that soil salinity could well be estimated by some of these VIs. Indices related to chlorophyll absorption bands or based on a combination of chlorophyll and water absorption bands had the highest correlation with soil salinity. In contrast, indices that are only based on water absorption bands had low to medium correlations, while indices that use only visible bands did not perform well. From the investigated indices the optimized soil-adjusted vegetation index (OSAVI) had the strongest relationship (R² = 0.69) with soil salinity for the training data, but it did not perform well in the validation phase. The validation procedure showed that the new salinity and water stress indices (SWSI) implemented in this study (SWSI-1, SWSI-2, SWSI-3) and the Vogelmann red edge index yielded the best results for estimating soil salinity for independent fields with root mean square errors of 1.14, 1.15, 1.17 and 1.15 dS/m, respectively. Our results show that soil salinity could be estimated by satellite-based hyperspectral VIs, but validation of obtained models for independent data is essential for selecting the best model.     

Evaluating the robustness of models developed from field spectral data in predicting African grass foliar nitrogen concentration using WorldView-2 image as an independent test dataset

In this paper, we evaluate the extent to which the resampled field spectra compare with the actual image spectra of the new generation multispectral WorldView-2 (WV-2) satellite. This was achieved by developing models from resampled field spectra data and testing them on an actual WV-2 image of the study area. We evaluated the performance of reflectance ratios (RI), normalized difference indices (NDI) and random forest (RF) regression model in predicting foliar nitrogen concentration in a grassland environment. The field measured spectra were used to calibrate the RF model using a randomly selected training (n = 70%) nitrogen data set. The model developed from the field spectra resampled to WV-2 wavebands was validated on an independent field spectral test dataset as well as on the actual WV-2 image of the same area (n = 30%, bootstrapped a 100 times). The results show that the model developed using RI could predict nitrogen with a mean R² of 0.74 and 0.65 on an independent field spectral test data set and on the actual WV-2 image, respectively. The root mean square error of prediction (RMSE %) was 0.17 and 0.22 for the field test data set and the WV-2 image, respectively. Results provide an insight on the magnitude of errors that are expected when up-scaling field spectral models to airborne or satellite image data. The prediction also indicates the unceasing relevance of field spectroscopy studies to better understand the spectral models critical for vegetation quality assessment.     

Different units of measurement of carotenoids estimation in cotton using hyperspectral indices and partial least square regression

This paper examines the use of canopy reflectance for different units of measurements of carotenoids estimation. Field spectral measurements were collected over cotton in different intensive field campaigns organized during the growing seasons of 2010 and 2011. Three units of measurement were evaluated carotenoids expressed as a mass per unit soil surface area (g/m²), a mass per unit leaf area (μg/cm²), and a mass per unit fresh leaf weight (mg/g), respectively. Four methods were compared to retrieve amount of carotenoids: stepwise multiple linear regression (SMLR), published spectral indices, band combination indices, and partial least square regression (PLSR). Results show that maximum sensitivity of reflectance to variation in different units of measurement of carotenoids was found in the green region at 515–550 nm, and at 715 nm and 750 nm regions in the far-red wavelengths. The predictive accuracies of Car (g/m²), Car (μg/cm²) and Car (mg/g) were tested on a validation data set and the results show that the highest R² values between estimations and observations were 0.468 for Car (g/m²), 0.563 for Car (μg/cm²), and 0.456 for Car (mg/g), with relative root mean square error (RMSE%, RMSE/mean) of 48.72%, 22.07% and 21.07%, respectively. Compared to Car (g/m²) and Car (mg/g), the model performance indices for Car (μg/cm²) show a high degree of consistency among the R² values and RMSE% and MAE% values. Further comparison were performed among the estimation accuracies of different unit carotenoids and among the different approaches used in the study by a paired-t-test. The results indicate that although the best estimation results for Car (μg/cm²) and Car (mg/g) were both obtained based on PLSR, they can be estimated by all four adopted methods without significant differences (P > 0.1). Whereas for Car (g/m²), the best estimation results were obtained based on published vegetation indices CIred-edge, which were significantly better than the estimation results based on SMLR (P < 0.000). In summary, the results of this study show that even the carotenoids expressed on concentration (mg/g) or content (μg/cm²) basis at leaf level can be estimated with the same prediction accuracies to the carotenoids expressed as a mass per unit surface area (g/m²) at canopy level using reflectance measurement at canopy level.     

Remotely detecting canopy nitrogen concentration and uptake of paddy rice in the Northeast China Plain

The influence of morphophysiological variation at different growth stages on the performance of vegetation indices for estimating plant N status has been confirmed. However, the underlying mechanisms explaining how this variation impacts hyperspectral measures and canopy N status are poorly understood. In this study, four field experiments involving different N rates were conducted to optimize the selection of sensitive bands and evaluate their performance for modeling canopy N status of rice at various growth stages in 2007 and 2008. The results indicate that growth stages negatively affect hyperspectral indices in different ways in modeling leaf N concentration (LNC), plant N concentration (PNC) and plant N uptake (PNU). Published hyperspectral indices showed serious limitations in estimating LNC, PNC and PNU. The newly proposed best 2-band indices significantly improved the accuracy for modeling PNU (R² = 0.75–0.85) by using the lambda by lambda band-optimized algorithm. However, the newly proposed 2-band indices still have limitations in modeling LNC and PNC because the use of only 2-band indices is not fully adequate to provide the maximum N-related information. The optimum multiple narrow band reflectance (OMNBR) models significantly increase the accuracy for estimating the LNC (R² = 0.67–0.71) and PNC (R² = 0.57–0.78) with six bands. Results suggest the combinations of center of red-edge (735 nm) with longer red-edge bands (730–760 nm) are very efficient for estimating PNC after heading, whereas the combinations of blue with green bands are more efficient for modeling PNC across all stages. The center of red-edge (730–735 nm) paired with early NIR bands (775–808 nm) are predominant in estimating PNU before heading, whereas the longer red-edge (750 nm) paired with the center of “NIR shoulder” (840–850 nm) are dominant in estimating PNU after heading and across all stages. The OMNBR models have the advantage of modeling canopy N status for the entire growth period. However, the best 2-band indices are much easier to use. Alternatively, it is also possible to use the best 2-band indices to monitor PNU before heading and PNC after heading. This study systematically explains the influences of N dilution effect on hyperspectral band combinations in relating to the different N variables and further recommends the best band combinations which may provide an insight for developing new hyperspectral vegetation indices.     

Retrieval of tea polyphenol at leaf level using spectral transformation and multi-variate statistical approach

In the present study, field based hyperspectral data was used to estimate the tea (Camellia sinensis L.) polyphenol at Deha Tea garden of Assam state, India. Leaf reflectance spectra were first filtered for noise and then transformed into normalized and first derivative reflectance for further analysis. Stepwise discriminant analysis was carried out to select sensitive bands for a range of polyphenol concentration by minimizing the effects of other factors such as age of the bushes and management practices. The wavelengths at 358, 369, 484, 845, 916, 1387, 1420, 1435, 1621 and 2294 nm were identified as sensitive to tea polyphenol, among which 2294 nm was found to be the most recurring band. The noise removed selected bands, their transformed derivatives and principal components were regressed with the tea polyphenol using univariate and multi-variate analysis. In univariate analysis the correlation was very poor with RMSE more than 3.0. A significant improvement in R² values were observed when multivariate analyses like stepwise multiple linear regression (SMLR) and partial least square regression (PLSR) was carried out. The PLSR of first derivative reflectance was most accurate (R² = 0.81 and RMSE = 1.39 mg g⁻¹) among all the uni- and multivariate analysis for predicting the polyphenol of fresh tea leaves.     

Comparative analysis of different retrieval methods for mapping grassland leaf area index using airborne imaging spectroscopy

Fine scale maps of vegetation biophysical variables are useful status indicators for monitoring and managing national parks and endangered habitats. Here, we assess in a comparative way four different retrieval methods for estimating leaf area index (LAI) in grassland: two radiative transfer model (RTM) inversion methods (one based on look-up-tables (LUT) and one based on predictive equations) and two statistical modelling methods (one partly, the other entirely based on in situ data). For prediction, spectral data were used that had been acquired over Majella National Park in Italy by the airborne hyperspectral HyMap instrument. To assess the performance of the four investigated models, the normalized root mean squared error (nRMSE) and coefficient of determination (R²) between estimates and in situ LAI measurements are reported (n = 41). Using a jackknife approach, we also quantified the accuracy and robustness of empirical models as a function of the size of the available calibration data set. The results of the study demonstrate that the LUT-based RTM inversion yields higher accuracies for LAI estimation (R² = 0.91, nRMSE = 0.18) as compared to RTM inversions based on predictive equations (R² = 0.79, nRMSE = 0.38). The two statistical methods yield accuracies similar to the LUT method. However, as expected, the accuracy and robustness of the statistical models decrease when the size of the calibration database is reduced to fewer samples. The results of this study are of interest for the remote sensing community developing improved inversion schemes for spaceborne hyperspectral sensors applicable to different vegetation types. The examples provided in this paper may also serve as illustrations for the drawbacks and advantages of physical and empirical models.     

Modeling soil parameters using hyperspectral image reflectance in subtropical coastal wetlands

Developing spectral models of soil properties is an important frontier in remote sensing and soil science. Several studies have focused on modeling soil properties such as total pools of soil organic matter and carbon in bare soils. We extended this effort to model soil parameters in areas densely covered with coastal vegetation. Moreover, we investigated soil properties indicative of soil functions such as nutrient and organic matter turnover and storage. These properties include the partitioning of mineral and organic soil between particulate (>53 μm) and fine size classes, and the partitioning of soil carbon and nitrogen pools between stable and labile fractions. Soil samples were obtained from Avicennia germinans mangrove forest and Juncus roemerianus salt marsh plots on the west coast of central Florida. Spectra corresponding to field plot locations from Hyperion hyperspectral image were extracted and analyzed. The spectral information was regressed against the soil variables to determine the best single bands and optimal band combinations for the simple ratio (SR) and normalized difference index (NDI) indices. The regression analysis yielded levels of correlation for soil variables with R² values ranging from 0.21 to 0.47 for best individual bands, 0.28 to 0.81 for two-band indices, and 0.53 to 0.96 for partial least-squares (PLS) regressions for the Hyperion image data. Spectral models using Hyperion data adequately (RPD > 1.4) predicted particulate organic matter (POM), silt + clay, labile carbon (C), and labile nitrogen (N) (where RPD = ratio of standard deviation to root mean square error of cross-validation [RMSECV]). The SR (0.53 μm, 2.11 μm) model of labile N with R² = 0.81, RMSECV= 0.28, and RPD = 1.94 produced the best results in this study. Our results provide optimism that remote-sensing spectral models can successfully predict soil properties indicative of ecosystem nutrient and organic matter turnover and storage, and do so in areas with dense canopy cover.     

Assessment of RapidEye vegetation indices for estimation of leaf area index and biomass in corn and soybean crops

Leaf area index (LAI) and biomass are important indicators of crop development and the availability of this information during the growing season can support farmer decision making processes. This study demonstrates the applicability of RapidEye multi-spectral data for estimation of LAI and biomass of two crop types (corn and soybean) with different canopy structure, leaf structure and photosynthetic pathways. The advantages of Rapid Eye in terms of increased temporal resolution (∼daily), high spatial resolution (∼5 m) and enhanced spectral information (includes red-edge band) are explored as an individual sensor and as part of a multi-sensor constellation. Seven vegetation indices based on combinations of reflectance in green, red, red-edge and near infrared bands were derived from RapidEye imagery between 2011 and 2013. LAI and biomass data were collected during the same period for calibration and validation of the relationships between vegetation indices and LAI and dry above-ground biomass. Most indices showed sensitivity to LAI from emergence to 8 m²/m². The normalized difference vegetation index (NDVI), the red-edge NDVI and the green NDVI were insensitive to crop type and had coefficients of variations (CV) ranging between 19 and 27%; and coefficients of determination ranging between 86 and 88%. The NDVI performed best for the estimation of dry leaf biomass (CV = 27% and r² = 090) and was also insensitive to crop type. The red-edge indices did not show any significant improvement in LAI and biomass estimation over traditional multispectral indices. Cumulative vegetation indices showed strong performance for estimation of total dry above-ground biomass, especially for corn (CV ≤ 20%). This study demonstrated that continuous crop LAI monitoring over time and space at the field level can be achieved using a combination of RapidEye, Landsat and SPOT data and sensor-dependant best-fit functions. This approach eliminates/reduces the need for reflectance resampling, VIs inter-calibration and spatial resampling.     

Hyperspectral-based predictive modelling of grapevine water status in the Portuguese Douro wine region

In this study, hyperspectral reflectance (HySR) data derived from a handheld spectroradiometer were used to assess the water status of three grapevine cultivars in two sub-regions of Douro wine region during two consecutive years. A large set of potential predictors derived from the HySR data were considered for modelling/predicting the predawn leaf water potential (Ψ_pd) through different statistical and machine learning techniques. Three HySR vegetation indices were selected as final predictors for the computation of the models and the in-season time trend was removed from data by using a time predictor. The vegetation indices selected were the Normalized Reflectance Index for the wavelengths 554 nm and 561 nm (NRI_554;561), the water index (WI) for the wavelengths 900 nm and 970 nm, and the D1 index which is associated with the rate of reflectance increase in the wavelengths of 706 nm and 730 nm. These vegetation indices covered the green, red edge and the near infrared domains of the electromagnetic spectrum. A large set of state-of-the-art analysis and statistical and machine-learning modelling techniques were tested. Predictive modelling techniques based on generalized boosted model (GBM), bagged multivariate adaptive regression splines (B-MARS), generalized additive model (GAM), and Bayesian regularized neural networks (BRNN) showed the best performance for predicting Ψ_pd, with an average determination coefficient (R²) ranging between 0.78 and 0.80 and RMSE varying between 0.11 and 0.12 MPa. When cultivar Touriga Nacional was used for training the models and the cultivars Touriga Franca and Tinta Barroca for testing (independent validation), the models performance was good, particularly for GBM (R² = 0.85; RMSE = 0.09 MPa). Additionally, the comparison of Ψ_pd observed and predicted showed an equitable dispersion of data from the various cultivars. The results achieved show a good potential of these predictive models based on vegetation indices to support irrigation scheduling in vineyard.     

Above ground biomass estimation in an African tropical forest with lidar and hyperspectral data

The estimation of above ground biomass in forests is critical for carbon cycle modeling and climate change mitigation programs. Small footprint lidar provides accurate biomass estimates, but its application in tropical forests has been limited, particularly in Africa. Hyperspectral data record canopy spectral information that is potentially related to forest biomass. To assess lidar ability to retrieve biomass in an African forest and the usefulness of including hyperspectral information, we modeled biomass using small footprint lidar metrics as well as airborne hyperspectral bands and derived vegetation indexes. Partial Least Square Regression (PLSR) was adopted to cope with multiple inputs and multicollinearity issues; the Variable of Importance in the Projection was calculated to evaluate importance of individual predictors for biomass. Our findings showed that the integration of hyperspectral bands (R² = 0.70) improved the model based on lidar alone (R² = 0.64), this encouraging result call for additional research to clarify the possible role of hyperspectral data in tropical regions. Replacing the hyperspectral bands with vegetation indexes resulted in a smaller improvement (R² = 0.67). Hyperspectral bands had limited predictive power (R² = 0.36) when used alone. This analysis proves the efficiency of using PLSR with small-footprint lidar and high resolution hyperspectral data in tropical forests for biomass estimation. Results also suggest that high quality ground truth data is crucial for lidar-based AGB estimates in tropical African forests, especially if airborne lidar is used as an intermediate step of upscaling field-measured AGB to a larger area.     

Combining UAV-based plant height from crop surface models,visible, and near infrared vegetation indices for biomass monitoring in barley

In this study we combined selected vegetation indices (VIs) and plant height information to estimate biomass in a summer barley experiment. The VIs were calculated from ground-based hyperspectral data and unmanned aerial vehicle (UAV)-based red green blue (RGB) imaging. In addition, the plant height information was obtained from UAV-based multi-temporal crop surface models (CSMs). The test site is a summer barley experiment comprising 18 cultivars and two nitrogen treatments located in Western Germany. We calculated five VIs from hyperspectral data. The normalised ratio index (NRI)-based index GnyLi (Gnyp et al., 2014) showed the highest correlation (R² = 0.83) with dry biomass. In addition, we calculated three visible band VIs: the green red vegetation index (GRVI), the modified GRVI (MGRVI) and the red green blue VI (RGBVI), where the MGRVI and the RGBVI are newly developed VI. We found that the visible band VIs have potential for biomass prediction prior to heading stage. A robust estimate for biomass was obtained from the plant height models (R² = 0.80–0.82). In a cross validation test, we compared plant height, selected VIs and their combination with plant height information. Combining VIs and plant height information by using multiple linear regression or multiple non-linear regression models performed better than the VIs alone. The visible band GRVI and the newly developed RGBVI are promising but need further investigation. However, the relationship between plant height and biomass produced the most robust results. In summary, the results indicate that plant height is competitive with VIs for biomass estimation in summer barley. Moreover, visible band VIs might be a useful addition to biomass estimation. The main limitation is that the visible band VIs work for early growing stages only.     

Detecting forest damage after a low-severity fire using remote sensing at multiple scales

Remote sensing technologies are an ideal platform to examine the extent and impact of fire on the landscape. In this study we assess that capacity of the RapidEye constellation and Landsat (Thematic Mapper and Operational Land Imager to map fine-scale burn attributes for a small, low severity prescribed fire in a dry Western Canadian forest. Estimates of burn severity from field data were collated into a simple burn index and correlated with a selected suite of common spectral vegetation indices. Burn severity classes were then derived to map fire impacts and estimate consumed woody surface fuels (diameter ≥2.6 cm). All correlations between the simple burn index and vegetation indices produced significant results (p < 0.01), but varied substantially in their overall accuracy. Although the Landsat Soil Adjusted Vegetation Index provided the best regression fit (R² = 0.56), results suggested that RapidEye provided much more spatially detailed estimates of tree damage (Soil Adjusted Vegetation Index, R² = 0.51). Consumption estimates of woody surface fuels ranged from 3.38 ± 1.03 Mg ha⁻¹ to 11.73 ± 1.84 Mg ha⁻¹, across four derived severity classes with uncertainties likely a result of changing foliage moisture between the before and after fire images. While not containing spectral information in the short wave infrared, the spatial variability provided by the RapidEye imagery has potential for mapping and monitoring fine scale forest attributes, as well as the potential to resolve fire damage at the individual tree level.     

Modelling high arctic percent vegetation cover using field digital images and high resolution satellite data

In this study, digital images collected at a study site in the Canadian High Arctic were processed and classified to examine the spatial-temporal patterns of percent vegetation cover (PVC). To obtain the PVC of different plant functional groups (i.e., forbs, graminoids/sedges and mosses), field near infrared-green-blue (NGB) digital images were classified using an object-based image analysis (OBIA) approach. The PVC analyses comparing different vegetation types confirmed: (i) the polar semi-desert exhibited the lowest PVC with a large proportion of bare soil/rock cover; (ii) the mesic tundra cover consisted of approximately 60% mosses; and (iii) the wet sedge consisted almost exclusively of graminoids and sedges. As expected, the PVC and green normalized difference vegetation index (GNDVI; (R_NIR − R_Green)/(R_NIR + R_Green)), derived from field NGB digital images, increased during the summer growing season for each vegetation type: i.e., ∼5% (0.01) for polar semi-desert; ∼10% (0.04) for mesic tundra; and ∼12% (0.03) for wet sedge respectively. PVC derived from field images was found to be strongly correlated with WorldView-2 derived normalized difference spectral indices (NDSI; (R_x − R_y)/(R_x + R_y)), where R_x is the reflectance of the red edge (724.1 nm) or near infrared (832.9 nm and 949.3 nm) bands; R_y is the reflectance of the yellow (607.7 nm) or red (658.8 nm) bands with R²’s ranging from 0.74 to 0.81. NDSIs that incorporated the yellow band (607.7 nm) performed slightly better than the NDSIs without, indicating that this band may be more useful for investigating Arctic vegetation that often includes large proportions of senescent vegetation throughout the growing season.     

Evaluating the relationship between biomass,percent groundcover and remote sensing indices across six winter cover crop fields in Maryland,United States

Winter cover crops are an essential part of managing nutrient and sediment losses from agricultural lands. Cover crops lessen sedimentation by reducing erosion, and the accumulation of nitrogen in aboveground biomass results in reduced nutrient runoff. Winter cover crops are planted in the fall and are usually terminated in early spring, making them susceptible to senescence, frost burn, and leaf yellowing due to wintertime conditions. This study sought to determine to what extent remote sensing indices are capable of accurately estimating the percent groundcover and biomass of winter cover crops, and to analyze under what critical ranges these relationships are strong and under which conditions they break down. Cover crop growth on six fields planted to barley, rye, ryegrass, triticale or wheat was measured over the 2012–2013 winter growing season. Data collection included spectral reflectance measurements, aboveground biomass, and percent groundcover. Ten vegetation indices were evaluated using surface reflectance data from a 16-band CROPSCAN sensor. Restricting analysis to sampling dates before the onset of prolonged freezing temperatures and leaf yellowing resulted in increased estimation accuracy. There was a strong relationship between the normalized difference vegetation index (NDVI) and percent groundcover (r² = 0.93) suggesting that date restrictions effectively eliminate yellowing vegetation from analysis. The triangular vegetation index (TVI) was most accurate in estimating high ranges of biomass (r² = 0.86), while NDVI did not experience a clustering of values in the low and medium biomass ranges but saturated in the higher range (>1500 kg/ha). The results of this study show that accounting for index saturation, senescence, and frost burn on leaves can greatly increase the accuracy of estimates of percent groundcover and biomass for winter cover crops.     

Assessment of leaf carotenoids content with a new carotenoid index: Development and validation on experimental and model data

Leaf carotenoids content (LCar) is an important indicator of plant physiological status. Accurate estimation of LCar provides valuable insight into early detection of stress in vegetation. With spectroscopy techniques, a semi-empirical approach based on spectral indices was extensively used for carotenoids content estimation. However, established spectral indices for carotenoids that generally rely on limited measured data, might lack predictive accuracy for carotenoids estimation in various species and at different growth stages. In this study, we propose a new carotenoid index (CARI) for LCar assessment based on a large synthetic dataset simulated from the leaf radiative transfer model PROSPECT-5, and evaluate its capability with both simulated data from PROSPECT-5 and 4SAIL and extensive experimental datasets: the ANGERS dataset and experimental data acquired in field experiments in China in 2004. Results show that CARI was the index most linearly correlated with carotenoids content at the leaf level using a synthetic dataset (R² = 0.943, RMSE = 1.196 μg/cm²), compared with published spectral indices. Cross-validation results with CARI using ANGERS data achieved quite an accurate estimation (R² = 0.545, RMSE = 3.413 μg/cm²), though the RBRI performed as the best index (R² = 0.727, RMSE = 2.640 μg/cm²). CARI also showed good accuracy (R² = 0.639, RMSE = 1.520 μg/cm²) for LCar assessment with leaf level field survey data, though PRI performed better (R² = 0.710, RMSE = 1.369 μg/cm²). Whereas RBRI, PRI and other assessed spectral indices showed a good performance for a given dataset, overall their estimation accuracy was not consistent across all datasets used in this study. Conversely CARI was more robust showing good results in all datasets. Further assessment of LCar with simulated and measured canopy reflectance data indicated that CARI might not be very sensitive to LCar changes at low leaf area index (LAI) value, and in these conditions soil moisture influenced the LCar retrieval accuracy.     

Retrieval of leaf water content spanning the visible to thermal infrared spectra

The objective of this study was to investigate the entire spectra (from visible to the thermal infrared; 0.390–14.0 μm) to retrieve leaf water content in a consistent manner. Narrow-band spectral indices (calculated from all possible two band combinations) and a partial least square regression (PLSR) were used to assess the strength of each spectral region. The coefficient of determination (R²) and root mean square error (RMSE) were used to report the prediction accuracy of spectral indices and PLSR models. In the visible-near infrared and shortwave infrared (VNIR–SWIR), the most accurate spectral index yielded R² of 0.89 and RMSE of 7.60%, whereas in the mid infrared (MIR) the highest R² was 0.93 and RMSE of 5.97%. Leaf water content was poorly predicted using two-band indices developed from the thermal infrared (R² = 0.33). The most accurate PLSR model resulted from MIR reflectance spectra (R² = 0.96, RMSE = 4.74% and RMSE cross validation RMSE_CV = 6.17%) followed by VNIR–SWIR reflectance spectra (R² = 0.91, RMSE = 6.90% and RMSE_CV = 7.32%). Using thermal infrared (TIR) spectra, the PLSR model yielded a moderate retrieval accuracy (R² = 0.67, RMSE = 13.27% and RMSE_CV = 16.39%). This study demonstrated that the mid infrared (MIR) and shortwave infrared (SWIR) domains were the most sensitive spectral region for the retrieval of leaf water content.     

Exploring hyperspectral bands and estimation indices for leaf nitrogen accumulation in wheat

Hyperspectral sensing can provide an effective means for fast and non-destructive estimation of leaf nitrogen (N) status in crop plants. The objectives of this study were to design a new method to extract hyperspectral spectrum information, to explore sensitive spectral bands, suitable bandwidth and best vegetation indices based on precise analysis of ground-based hyperspectral information, and to develop regression models for estimating leaf N accumulation per unit soil area (LNA, g N m⁻²) in winter wheat (Triticum aestivum L.). Three field experiments were conducted with different N rates and cultivar types in three consecutive growing seasons, and time-course measurements were taken on canopy hyperspectral reflectance and LNA under the various treatments. Then, normalized difference spectral indices (NDSI) and ratio spectral indices (RSI) based on the original spectrum and the first derivative spectrum were constructed within the range of 350–2500 nm, and their relationships with LNA were quantified. The results showed that both LNA and canopy hyperspectral reflectance in wheat changed with varied N rates, with consistent patterns across different cultivars and seasons. The sensitive spectral bands for LNA existed mainly within visible and near infrared regions. The best spectral indices for estimating LNA in wheat were found to be NDSI (R₈₆₀, R₇₂₀), RSI (R₉₉₀, R₇₂₀), NDSI (FD₇₃₆, FD₅₂₆) and RSI (FD₇₂₅, FD₅₁₆), and the regression models based on the above four spectral indices were formulated as Y = 26.34x^1.887, Y = 5.095x − 6.040, Y = 0.609 e^3.008x and Y = 0.388x^1.260, respectively, with R² greater than 0.81. Furthermore, expanding the bandwidth of NDSI (R₈₆₀, R₇₂₀) and RSI (R₉₉₀, R₇₂₀) from 1 nm to 100 nm at 1 nm interval produced the LNA monitoring models with similar performance within about 33 nm and 23 nm bandwidth, respectively, over which the statistical parameters of the models became less stable. From testing of the derived equations, the model for LNA estimation on NDSI (R₈₆₀, R₇₂₀), RSI (R₉₉₀, R₇₂₀), NDSI (FD₇₃₆, FD₅₂₆) and RSI (FD₇₂₅, FD₅₁₆) gave R² over 0.79 with more satisfactory performance than previously reported models and physical models in wheat. It can be concluded that the present hyperspectral parameters of NDSI (R₈₆₀, R₇₂₀), RSI (R₉₉₀, R₇₂₀), NDSI (FD₇₃₆, FD₅₂₆) and RSI (FD₇₂₅, FD₅₁₆) can be reliably used for estimating LNA in winter wheat.     

Estimation of higher chlorophylla concentrations using field spectral measurement and HJ-1A hyperspectral satellite data in Dianshan Lake,China

Based on in situ water sampling and field spectral measurements in Dianshan Lake, a semi-analytical three-band algorithm was used to estimate Chlorophylla (Chla) content in case II waters. The three bands selected to estimate Chla for high concentrations included 653, 691 and 748 nm. An equation, based on the difference in reciprocal reflectance between 653 and 691 nm, multiplied by reflectance at 748 nm as [R_rs⁻¹(653) − R_rs⁻¹ (691)] R_rs(748), explained 85.57% of variance in Chla concentration with a root mean square error (RMSE) of <6.56 mg/m³. In order to test the utility of this model with satellite data, HJ-1A Hyperspectral Imager (HSI) data were analyzed using comparable wavelengths selected from the in situ data [B₆₇⁻¹(656) − B₈₀⁻¹(716)] B₈₇(753). This model accounted for 84.3% of Chla variation, estimating Chla concentrations with an RMSE of <4.23 mg/m³. The results illustrate that, based on the determined wavelengths, the spectrum-based model can achieve a high estimation accuracy and can be applied to hyperspectral satellite imagery especially for higher Chla concentration waters.