Isolation and identification of a bacterium from marine shrimp digestive tract: A new degrader of starch and protein

It is a practical approach to select candidate probiotic bacterial stains on the basis of their special traits. Production of digestive enzyme was used as a trait to select a candidate probiotic bacterial strain in this study. In order to select a bacterium with the ability to degrade both starch and protein, an ideal bacterial strain STE was isolated from marine shrimp (Litopenaeus vannamei) intestines by using multiple selective media.The selected isolate STE was identified on the basis of its morphological, physiological,and biochemical characteristics as well as molecular analyses. Results of degradation experiments confirmed the ability of the selected isolate to degrade both starch and casein. The isolate STE was aerobic, Gram-negative, rod-shaped, motile and non-spore-forming, and had catalase and oxidase activities but no glucose fermentation activity. Among the tested carbon/nitrogen sources, only Tween40, alanyl-glycine, aspartyl-glycine, and glycyl-l-glutamic acid were utilized by the isolate STE. Results of homology comparison analyses of the 16S rDNA sequences showed that the isolate STE had a high similarity to several Pseudoalteromonas species and, in the phylogenetic tree, grouped with P. ruthenica with maximum bootstrap support (100%). In conclusion, the isolate STE was characterized as a novel strain belonging to the genus Pseudoalteromonas.This study provides a further example of a probiotic bacterial strain with specific characteristics isolated from the host gastrointestinal tract.     

一种变形监测中中长基线的单历元模糊度解算算法

针对已有单历元模糊度解算算法只适用于短基线的情形,根据误差传播率确定模糊度精度,依据误差确定模糊度搜索空间,提出一种利用GPS双频观测信息实现中长基线下单历元模糊度固定的算法。首先,利用电离层约束法得到双差模糊度之间的线性约束条件;其次,利用变形监测中监测站点坐标近似已知的条件进行约束,反算获得近似模糊度;最后,根据监测站点已有的观测数据精度给出搜索空间,搜索固定模糊度。结果表明,该方法可以实现中长基线下单历元的模糊度固定,对实时变形监测有一定的参考价值。