中国对虾黄、渤海沿岸地理群的RAPD分析

采用随机扩增多态DNA(random amplified polymorphic DNA,简称RAPD)技术,对中国对虾的黄、渤海地理群的32个个体遗传多样性进行了检测,使用OPI系列的20个10bp的寡核苷酸随机引物,对每个个体的基因组DNA进行扩增,结果为:17个引物获得了谱带清晰且重复性好的产物,扩增片段的分子量在2000～2000bp之间,共检测106个位点,其中多态位点数为39个,占36.8%;平均遗传距离为0.0941+0.0206,有67个标记(占63.2%)在整个群体的32个个体间表观稳定的一致性.     

Exploring composition and behaviour of fish fauna by in situ observations in the Bari Canyon (Southern Adriatic Sea,Central Mediterranean)

Canyons play a fundamental role in enhancing the abundance and diversity of marine organisms through the transport of organic matter and food resources, the presence of complex physical habitats and the absence of trawl fishing. During four baited lander deployments carried out in the Bari Canyon (Southern Adriatic Sea, Central Mediterranean), at depths of 443–788 m, about 43 h of video records were taken, for a total of 619,200 video frames. A total of 12 benthopelagic fish species (five chondrichthyes and seven osteichthyes) were identified. The blackspot seabream (Pagellus bogaraveo) was the most often observed fish species. The depth of 787 m represents a new depth record for this fish in the Adriatic Sea. Groups of up to 40 individuals of P. bogaraveo were attracted to the bait and were shown in single frames. The individuals were observed both exploring the area and feeding actively on the bait. The European conger (Conger conger) was recorded at each deployment. Clear scavenger behaviour was also observed in this teleost fish and in the shark Etmopterus spinax. The shark species Centrophorus granulosus and Hexanchus griseus, which are considered ‘vulnerable’ on the published IUCN Mediterranean Regional Red List, were also recorded but, although attracted by the bait, they were never seen feeding on it. Other fish species, harvested on fishing grounds, such as Merluccius merluccius, Helicolenus dactylopterus and Polyprion americanus, were also recorded. This study represents the first in situ documentation, at very low impact, of the fish fauna in the Bari Canyon, providing new insights into its small scale distribution and behaviour, the first in situ direct observation of the variable feeding behaviour of P. bogaraveo and its gregarious habits, as well as indicating that this canyon could act as a refuge area for species that are vulnerable to fishing on the open slope.     

RAPD analysis of wild stock of penaeid shrimp (Penaeus chinensis) in Chinese coastal waters of the Huanghai Sea and coastal waters of the Bohai Sea

Intermsoftheresearchingermplasmandgeneticsofmarineanimals,Chinaseemsquiteweakincomparisonwithotherdevelopedcountries,fortherelevantresearchprogramswereonlyinitiatedinthe1980's.Inrecentyears,moreattentionhasbeendrawntothegermplasmsourcesofmarinevaluableanimalsandsomeresearchprogramshavebeenplannedorundertakeninChina.Abroad,thetechniquessuchaselectrophoresispatternsofisoenzym,RAPDandRFLPhavebeenappliedtotherelevantstudiesongeneticvariationandevolutionaryrelationshipswithinagroupofPenaeidshri…     

Parentage determination of black sea bream(Acanthopagrus schlegelii) for stock enhancement:effectiveness and loss of genetic variation

The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a fewyears.However,little information has been reported concerning the effectiveness and genetic effect of black sea bream stock enhancement.In order to detect the contribution of released individuals in Zhujiang River Estuary(ZRE) and Daya Bay(DB),six microsatellite markers were used to identify the hatchery-released individuals.In addition,this pedigree of hatchery populations(broodfish and hatchery-released offspring) was traced to detect the number of effective parents(N_e),the inbreeding coefficient and the decrease of genetic variability in the reproduction.The pedigree reconstruction showed that at least 69(out of 93) broodfish had offspring.The estimated N_e was 54.8,consequently the inbreeding coefficient was 0.91%.The genetic diversity of hatchery-released offspring was lower than that in that of broodfish(heterozygosity alleles,0.727-0.774),some alleles(number of alleles,61-69) and genetic variance were lost during reproduction.It was observed that wild samples had higher levels of genetic diversity compared with hatchery populations as well as recaptured samples in releasing area.A total of 128 hatchery-released black sea bream were identified among 487 recaptured samples in ZRE,while a total of 15 samples were identified among 96 samples in DB.In summary,there was a high survival of released fish.Nevertheless,the results provided evidence to consider a loss of genetic variation in hatcheryreleased stock and a negative genetic effect of the stock enhancement.     

Morphometric variation in the cutlassfish Trichiurus lepturus on the Kenyan coast: implications for stock identification and management

Morphometric variation was used to study population structure of the cutlassfish Trichiurus lepturus on the Kenyan coast. In all, 16 morphometric measurements taken from 193 individuals from six sites were subjected to multivariate statistical analysis in order to determine possible stock differentiation. Multivariate analysis of variance indicated significant differences in the means of a combination of the morphometric characters among the sites, and discriminant function analysis by groups showed the presence of phenotypically distinct populations on the south and north coasts of Kenya. A classification tree analysis of morphometric characters validated the distinct groups and indicated certain characters to be useful for classifying specimens to source sites. The likely presence of distinct north and south stocks of T. lepturus on the Kenyan coast observed in our study will require validation by other methods, but indicates the potential need for spatially explicit models for managing fish populations on a large spatial scale.     

Establishment and closure of the directed Florida whiting (Menticirrhus americanus) fishery and implications for North Atlantic right whale (Eubalaena glacialis) conservation and management

Today, ocean and coastal marine resource management is extremely complex. Marine resource managers are charged with conserving and managing many diverse species. Southern kingfish (Menticirrhus americanus), commonly known as whiting, are found from southern New England to Florida. During the fall through winter, western North Atlantic right whales (Eubalaena glacialis) are primarily found in the coastal nearshore waters off South Carolina, Georgia, and Florida, overlapping the whiting's range and habitat.     

基于微卫星(SSR)分子标记的中国对虾渤海湾增殖亲虾群体及回捕群体的遗传多样性分析

Eight microsatellite markers were used to analyze genetic diversity, level of inbreeding, and effective population size of spawner and recaptured populations of Chinese shrimp(Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay in 2013. A total of 254 and 238 alleles were identified in the spawner and recaptured populations, respectively, and the numbers of alleles(N_a) were 8–63 and 6–60, respectively. The numbers of effective alleles(N_e) were 2.52–21.60 and 2.67–20.72, respectively. The polymorphism information content ranged from 0.529 to 0.952. The observed heterozygosity(H_o) values(0.638–0.910 and 0.712–0.927) were lower than the expected heterozygosity(H_e) values(0.603–0.954 and 0.625–0.952), which indicated that the two populations possessed a rich genetic diversity. In 16 tests(2 populations×8 loci), 13 tests deviated from the HardyWeinberg equilibrium. F_(is) values were positive at seven loci and the inbreeding coefficients(F) of the two populations estimated by trio ML were 13.234% and 11.603%, suggesting that there was a relatively high degree of inbreeding. A certain level of inbreeding depression had occurred in the Chinese shrimp population. F_(st) values ranged from 0 to 0.059, with a mean of 0.028, displaying a low level of genetic differentiation in the two populations. Effective population sizes(3 060.2 and 3 842.8) were higher than the minimum number suggested for retaining the evolutionary potential to adapt to new environmental conditions. For enhancement activity in 2014,the ideal number of captured shrimp spawners should have ranged from 7 686 to 19 214 to maintain genetic diversity and effective population size. Further strategies to adjust the balance of economic cost, fishing effort and ideal number of shrimp spawners to maintain a satisfactory effective population size for ensuring the sustainability of Chinese shrimp are proposed.     

Quantifying the largest aggregation of giant trevally Caranx ignobilis (Carangidae) on record: implications for management

The giant trevally Caranx ignobilis (Forsskål) is an important apex predatory fish typically associated with coral reef communities. It is prized in recreational and commercial fisheries, yet little is known about its aggregation dynamics and susceptibility to fishing pressure. This study reports on a previously undocumented aggregation of mature giant trevally observed over a period of eight years (2010–2017) at Ponta do Ouro Partial Marine Reserve in southern Mozambique. The aggregation is one of the few recorded for this carangid in the western Indian Ocean and represents the first subtropical aggregation of giant trevally. The aggregation is also the largest recorded for this species, with up to 2 413 individuals representing an estimated biomass of approximately 30 tonnes. The size and predictability of this annual aggregation make it vulnerable to overexploitation and point towards the need for an appropriate conservation management strategy.     

Characterization of the southeastern US black sea bass (Centropristis striata) pot commercial fishery and implications for western North Atlantic right whale (Eubalaena glacialis) management and policy

A vital component of marine policy is the conservation and management of diverse marine resources. In the southeastern US, commercial fishermen target black sea bass (Centropristis striata) with pots from North Carolina to Cape Canaveral, Florida. During the fall through spring fishing season, western North Atlantic right whale (Eubalaena glacialis) distribution overlaps the black sea bass commercial pot fishery. Fishermen interviews revealed that the number of pots set ranged from 3 to 240 and the number of pots set per trawl ranged from 1 to 18. Generally, the amount of gear increased from south to north.     

Variability of the axial morphology and of the gravity structure along the Central Spreading Ridge (North Fiji Basin): evidence for contrasting thermal regimes

The Central Spreading Ridge (CSR) is located in the central part of the North Fiji Basin, a complex back-arc basin created 12 Ma ago between the Pacific and Indo-Australian plates. The 3.5 Ma old CSR is the best developed, for both structure and magmatism, of all the spreading centers identified in the basin, and may be one of the largest spreading systems of the west Pacific back-arc basins. It is more than 800 km long and 50–60 km wide, and has been intensively explored during the French-Japanese STARMER project (1987–1991).The CSR is segmented into three first order segments named, from north to south, N160°, N15° and N-S according to their orientation. This segmentation pattern is similar to that found at mid-ocean ridges. The calculated spreading rate is intermediate and ranges from 83 mm/yr at 20°30 S to 50 mm/yr at 17°S. In addition, there is a change in the axial ridge morphology and gravity structure between the northern and southern sections of the CSR. The axial morphology changes from a deep rift valley (N160° segment), to a dome split by an axial graben (N15° segment) and to a rectangular flat top high (N-S segment). The Mantle Bouguer Anomalies obtained on the northern part of the CSR (N160°/N15° segments) show bull's eye structures associated with mantle upwelling at the 16°50S triple junction and also in the middle of the segments. The Mantle Bouguer Anomalies of the southern part of the ridge (N-S segment) are more homogeneous and consistent with the observed smooth topography associated with axial isostatic compensation.At these intermediate spreading rates the contrast in bathymetry and gravity structure between the segments may reflect differences in heat supply. We suggest that the N160° and N15° segments are cold with respect to the hot N-S segment. We use a non-steady-state thermal model to test this hypothesis. In this model, the accretion is simulated as a nearly steady-state seafloor spreading upon which are superimposed periodic thermal inputs. With the measured spreading rate of 50 mm/yr, a cooling cycle of 200,000 yr develops a thermal state that permits to explain the axial morphology and gravity structure observed on the N160° segment. A spreading rate of 83 mm/yr and a cooling cycle of 120,000 yr would generate the optimal thermal structure to explain the characteristics of the N-S segment. The boundaries between the hot N-S segment and its cold bounding segments are the 18°10 S and 20°30 S propagating rifts. A heat propagation event along the N-S segment at the expense of the adjacent colder failing segments, can explain the sharp changes in the observed morphology and structure between the segments.     

The Effect of Temporal Changes and Environmental Trophic Condition on the Isotopic Composition (ω13C and ω15N) of Atherina boyeri (Risso, 1810) and Gobius niger (L., 1758) in a Mediterranean Coastal Lagoon (Lake of Sabaudia): Implications for Food Web Structure

Abstract. ω¹³C and ω¹⁵N of organic matter sources and consumers were employed to analyse trophic differentiation between a benthic consumer, Gobius niger (L., 1758) (Pisces, Osteichthyes), and a pelagic consumer, Atherina boyeri (Risso, 1810) (Pisces, Osteichthyes) in a Mediterranean coastal lagoon (Lake of Sabaudia) in winter and summer 1999. Trophic differences between the two species throughout the two sampling periods were related to the environmental trophic condition (i. e. nutrient and phytopigment concentrations). Although these two fish have different habitats, they both exploited benthic organisms, above all in summer. When the nutrient and phytopigment concentrations were higher (summer), the most abundant benthic organisms were the main food sources for both species. In winter A. boyeri apparently shifted its feeding preferences to include zooplankton, confirming its opportunistic feeding strategy. Par-ticulate organic matter and algae seemed to play the main trophic role in the food web structure. Benthic invertebrates used both sources, while zooplankton seemed to rely exclusively on the particulate fraction. The overlapping and very depleted signatures of sedimentary and particulate organic matter highlights a strong link between sediments and water column, rarely observed in other aquatic ecosystems using stable isotopes. Such a finding further substantiates the importance of particulate organic matter as a food source in the study area.