Transitory hydrographic structures and distribution of fish larvae and neustonic crustaceans in the north-western Mediterranean

A hydrographic and zooplankton survey (neuston and water column) was performed over the Blanes submarine canyon (western Mediterranean) in June 1993. Abundance of fish and crab larvae was very low when compared with previous studies conducted in the same area. The horizontal distribution of fish and crab larvae whose adults inhabit the continental shelf was restricted to the coast. The offshore occurrence of low-salinity waters of continental origin, together with the absence of a component perpendicular to the coast in the system of currents observed throughout the study area, support the observed pattern of distribution. The occurrence of mesopelagic fish larvae and of the oceanic neustonic isopod Idotea metallica in some coastal stations may be associated with episodic inshore-offshore exchanges of water masses identified by drifting buoy trajectories.     

墨西哥湾海洋环境对蓝蟹(Callinectes sapidus)幼体分布和扩散的影响

本文以蓝蟹为例,研究海洋环境对甲壳动物幼体迁移规律和机制的影响。利用不规则三角形网格和有限体积模型(finite-volume coastal ocean model, FVCOM)耦合kinesis模型的方法,分析研究了墨西哥湾物理环境对蓝蟹(Callinectessapidus)幼体的分布和扩散途径的影响。蓝蟹在每年的四、五月份海水落潮期间产卵,通过跟踪算法从产卵区域沿着墨西哥湾海域进行模拟,获得了80天内蓝蟹幼体的粒子移动轨迹,记录并分析了幼体经过海域的盐度值。研究结果证明了该方法可有效模拟蓝蟹幼体在特定海域的迁移规律和扩散机制,进一步研究可为了解海洋物理环境对蓝蟹和其他渔业资源的影响提供借鉴。     

Aspects of larval,post-larval and juvenile ecology of Macrobrachium petersi (Hilgendorf) in the Keiskamma estuary,South Africa

Although Macrobrachium petersi has nine larval stages, only stage I and a minimal number of stage II M. petersi larvae were caught in the Keiskamma estuary. Stage I larvae undergo a vertical migration at night which is markedly influenced by salinity, especially under stratified conditions. Larvae remain in the water column on the ebb tide, a behavioural pattern which effectively carried them to favourable salinities for growth and development. Stage I larvae show an association with salt front regions. The sudden decline in larval abundance from stage I to stage II downstream from the front suggests a change from a pelagic to an epibenthic existence. Later larval stages failed to appear in the plankton. However, post-larvae were caught in the estuary and a juvenile migration from the estuary to freshwater was monitored.     

三疣梭子蟹(Portunus trituberculatus)幼体不同干露温度下死亡率的研究

通过设置15℃、20℃、25℃、30℃4个干露温度梯度,检测不同发育阶段三疣梭子蟹幼体的露空时间、死亡率、含水量、失水率及体重消耗率等指标,分析干露温度、发育阶段与幼体死亡率的关系。结果表明,各发育阶段的幼体死亡率均随干露时间的增加而升高(P<0.05);不同温度条件下,Ⅰ期幼蟹(CⅠ)的含水量、失水率和体重消耗率均高于Ⅱ期幼蟹(CⅡ)(P<0.05);CⅡ20℃时的露空时间最长,为11h,半致死时间为7—8h;30℃时最短,为2h;CⅠ15℃时的露空时间最短,为0.5h;20℃时最长,为4h。相同温度下,幼体各发育阶段的耐干露能力为CⅡ>CⅠ>ZⅣ>ZⅢ=M。结果说明,温度≤15℃不利于幼体的存活,温度≥25℃干露耐受性降低。因此,20℃更有利于幼体获得长存活时间。     

<Emphasis Type="Italic">Cochlodinium polykrikoides</Emphasis> red tide detection in the South Sea of Korea using spectral classification of MODIS data

To distinguish true red tide water (particularly Cochlodinium polykrikoides blooms) from non-red tide water (false satellite high chlorophyll water) in the South Sea of Korea, we developed a systematic classification method using spectral information from MODIS level products and applied it to five different harmful algal bloom events. Red tide and nonred tide waters were classified based on four different criteria. The first step revealed that the radiance peaks of potential red tide water occurred at 555 and 678 nm. The second step separated optically different waters that were influenced by relatively low and high contributions of colored dissolved organic matter (CDOM) (including detritus) to chlorophyll. The third and fourth steps discriminated red tide water from non-red tide water based on the blue-to-green ratio in areas with lower and higher contributions of CDOM to chlorophyll, respectively. After applying the red tide classification (using the four criteria), the spectral response of the red tide water, which is influenced by pigment concentration, showed different slopes for the blue and green bands (lower slope at blue bands and higher slope at green bands). The opposite result was found for non-red tide water, due to decreasing phytoplankton absorption and increasing detritus/CDOM absorption at blue bands. The results were well matched with the discoloration of water (blue to dark red/brown) and delineated the areal coverage of C. polykrikoides blooms, revealing the nature of spatial and temporal variations in red tides. This simple spectral classification method led to increase user accuracy for C. polykrikoides and non-red tide blooms (>46% and >97%) and provided a more reliable and robust identification of red tides over a wide range of oceanic environments than was possible using chlorophyll a concentration, chlorophyll anomaly, fluorescence analysis, or proposed red tide detection algorithms.     

Detection of the water-soluble fraction of crude oil by the blue crab, Callinectes sapidus

The ability of the blue crab, Callinectes sapidus, to detect petroleum hydrocarbons was measured with behavioural techniques. When presented with a water-soluble fraction of Prudhoe Bay crude oil, blue crabs abruptly changed antennular orientation, began rhythmic beating of the maxillipedal flagellae, and increased antennular flicking rate. The threshold concentration at which 50% of the crabs detected the water-soluble fraction was 2 × 10^?6 mg/litre. The blue crab apparently can readily detect petroleum hydrocarbons at concentrations found in chronically polluted areas as well as oil spill situations.     

Spatial distribution and vertical migrations of fish larvae communities off Northwestern Iberia sampled with LHPR and Bongo nets

The spatial distribution and diel vertical migration of fish larvae were studied in relation to the environmental conditions off NW Iberia during May 2002. Larvae from 23 families were identified, the most abundant were the Clupeidae, Gobiidae, Callionymidae, Blenniidae, Sparidae and Labridae. Sardina pilchardus was the most abundant species, mean concentrations 1 order of magnitude higher than the other fish larvae species. Larval horizontal distribution was mainly related to upwelling-driven circulation, resulting in an offshore increase of larval abundance while the vertical distribution was closely associated to the Western Iberia Buoyant Plume. Despite this general trend, taxon-specific relationships between the distribution of larvae and environmental variables were observed, and temperature was an important regressor explaining the distribution of most taxa. A comparison between ichthyoplankton samples collected alternatively with the LHPR and Bongo nets resulted in captures of larvae ≈1 order of magnitude higher for the LHPR, probably related to its higher towing speed. The spatial distribution and relative composition of larvae were also different for both nets, although the most frequent/abundant groups were the same. A fixed station sampled for 69-h showed diel vertical migrations performed by the larvae, with the highest larval concentrations occurring at surface layers during the night and most larvae being found in the neuston layer only during that period.     

饵料对锯缘青蟹大眼幼体生长发育的影响

以不同比例的轮虫和卤虫无节幼体搭配成各种饵料组合，分别喂养不同发育期的锯缘青蟹幼体，重点研究大眼幼体的存活、生长发育、变态和干重及化学元素(C、H、N)含量的情况.结果表明饵料搭配得当，适时适量的投喂，其幼体的生长发育和变态都正常，并可获得较高的存活率，若饵料搭配不当不仅会导致幼体出现高死亡率，而且还能诱发发育期变化的现象；不同饵料搭配对大眼幼体干重及化学元素(C、H、N)含量的影响很显著，相差60％～70％，约一个发育期，而这差异可以通过该期幼体投喂足量卤虫无节幼体来加以弥补，大眼幼体的C、H、N占干重的百分比仅次于溞V，这表明青蟹幼体在这两次变态前需在体内积累高比例的有机营养物质来保证变态的顺利进行.     

Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone,China

Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological signi?cance in Shandong Province, China. Although B. liberate is crustacean, its external characteristics are not entirely those of shrimps or crabs. The question of whether B. liberate is a shrimp or a crab has been debated in recent years. We studied the karyotype of B. liberate by light microscopy using air-drying and spreading methods. We obtained mitotic chromosomal plates from B. liberate larvae, and from adult B. liberate females subsequent to egg-laying. The results revealed that B. liberate has 53 pairs of chromosomes(i.e., n =53 and 2 n =106), a characteristic shared with four species of crab. The karyogram of B. liberate consists of 25 metacentric, 14 submetacentric, 11 subtelocentric and 3 telocentric pairs. We did not ?nd any heteromorphosis sex chromosomes. Tissue from larvae, gills and ovaries can be used for chromosomal investigations, and we found similar lampbrush chromosomes in ovary cells. Comparatively speaking, larvae tissue is more practical, and ovary tissue is more suitable for the preparation of lampbrush chromosomes. B. liberate is more closely related to crabs than to shrimps, based on the numbers of chromosomes. The B. liberate karyotype reported here provides a basis for further comparative cytogenetic studies of species populations.     

Estuarine retention of larvae of the crab Rhithropanopeus harrisii

Larvae of estuarine organisms continually face possible export from the parent estuary. Retention of larvae of the estuarine crab Rhithropanopeus harrisii was investigated in the upper Newport River estuary, North Carolina. All of the developmental stages occurred in the same area of the estuary with similar horizontal distributions, and the concentrations of intermediate and late stages were not greatly reduced from those of the first larval stage. This was strong evidence for the continuous retention of larvae in the upper estuary.To determine mechanisms by which retention might be effected, field studies of the vertical distributions and migrations of these larvae were made. The four zoeal stages had similar but complex vertical migration patterns, which varied from study to study. These migrations centered on the depth of no net flow, reducing longitudinal transport during development. Cross-spectral analysis of the larval migrations and the environmental cycles of light, salinity and current speed revealed that each of these external cycles affected larval depth. Megalopae of R. harrisii also migrated vertically, but they were present in much lower concentrations than the zoeal stages, an indication of a change to benthic existence in this final larval form.     

Inter-annual variability in larval supply to populations of three invertebrate taxa in the northern California Current

We investigated sources of inter-annual variability in larval supply to crab and sea urchin populations at Bodega Head and Point Reyes in northern California. During the spring and summer upwelling seasons of the years 1992 through 1997 we monitored the weekly settlement rates of nine species of crabs and two species of sea urchins. As observed in previous studies, daily values of alongshore windstress, temperature and salinity provided evidence for the poleward flow of relatively warm, low salinity water from south of Point Reyes, an apparent retention zone, during upwelling relaxation events. In years dominated by these events (1992, 1993, 1995 and 1996) we observed that alongshore windstress, temperature and salinity were coherent and temperature was significantly correlated with cancrid crab settlement. During these years the magnitude of cancrid crab settlement and the fraction of cancrid crabs relative to other crab species settling were high. Over four years of concurrent sampling there was consistently greater cancrid crab settlement at the Point Reyes site, within the retention zone, than at Bodega Head. Settlement of non-cancrid crabs (porcellanids, grapsids, pagurids and majids) was not as closely linked to intra-annual patterns of upwelling and relaxation, possibly due to the shorter seasonal availability of larvae allowing for the influence of fewer relaxation events. Settlement of this group among years was positively correlated with environmental indicators of strong seasonal upwelling; high salinity, Bakun upwelling index and low temperature. Sea urchin settlement events were observed in June and July of 1992, 1994 and 1997 during warming periods when salinity and temperature were increasing and alongshore windstress was low. Across the six years of the study, we found that cancrid crab larvae had a more even seasonal availability than larvae of non-cancrid species, which settled in greatest numbers during the early portion of the upwelling season. Sea urchins settled in greatest numbers during the later part of the upwelling season. Together these patterns demonstrate the taxon-specific way that inter-annual variability in larval supply is forced by the coincidence of larval availability with favorable physical transport mechanisms.     

Tidal,diel and semi-lunar changes in the faunal assemblage of an intertidal salt marsh creek

The utilisation of a brackish estuarine marsh by nekton was investigated over a semi-lunar cycle in August 1994. Nekton migrating in and out of the intertidal creeks of the marsh ‘Het Verdronken Land van Saeftinghe’ in the Westerschelde estuary, SW Netherlands, was sampled passively during seven complete tidal cycles. Sampling one tidal cycle yielded three consecutive flood samples and four consecutive ebb samples. Sampling occurred every 2–3 days, covering diel, tidal and semi-lunar situations, thus allowing comparison of tidal, diel and semi-lunar influences on the composition of the intertidal fauna.Two different tidal-migration modes were observed. The mysid shrimp, Mesopodopsis slabberi, showed maximum abundance around high tide. For the remaining common species, the mysid (Neomysis integer), the shrimp (Palaemonetes varians), the crab (Carcinus maenas) and the goby (Pomatoschistus microps) and the amphipod (Corophium volutator), highest densities were recorded during lower water heights. The faunal assemblage shifts between the different tidal stages.On two occasions, consecutive day and night samples were taken. Total densities were higher during the night samples. During spring tide, difference in community composition was noticed between the night and the day samples. During neap tide, day–night differences were less clear. Recorded total densities were highest during spring tide and lowest during neap tide. At maximum water levels, a drop in total density was observed. A shift in community composition occurred between spring and neap tides.     

High environmental salinity reduces the reproductive potential of the spider crab Maja brachydactyla (Decapoda,Majidae)

The spider crab Maja brachydactyla is an important fisheries species in Europe. Adults were sampled in Ría de A Coruña (NW Spain) and transported to IRTA rearing facilities. Salinity and photoperiod changes and their effect on reproduction were tested in the laboratory. The number of newly hatched larvae per batch (presence of actively swimming newly hatched larvae in a broodstock tank) was counted and sampled to estimate dry weight and proximal composition. Keeping spider crab broodstock in a high salinity environment (38 ppt) significantly reduced the number of newly hatched larvae, reaching up to fivefold difference in winter. The number of newly hatched larvae produced under a natural photoperiod was 10‐fold higher than those produced using a fixed photoperiod at 34 ppt salinity during winter. No clear pattern of changes in larval dry weight, protein, lipid or carbohydrate composition was observed under the environmental conditions tested. The results show that the salinity used for broodstock maintenance, especially during the embryonic development, is an important factor causing changes in larval production of M. brachydactyla and with potential implications for recruitment success.     

Spatial distribution of the crab larvae (Decapoda: Anomura et Brachyura) in Possyet Bay (Peter the Great Bay of the Sea of Japan) in 2000–2001

The analysis of the data obtained during the plankton surveys in Possyet Bay (Peter the Great Bay of the Sea of Japan) in 2000–2001 is presented. The larvae of eight crab species were registered in the plankton: the red king crab Paralithodes camtschaticus (Tilesius, 1815), the snow (opilio) crab Chionoecetes opilio (Fabricius, 1780), the Arctic lyre crab Hyas coarctatus ursinus (Leach, 1815), the kelp crab Pugettia quadridens (de Haan, 1839), the helmet crab Telmessus cheiragonus (Tilesius, 1815), the Japanese swimming crab Charybdis japonica (A. Milne-Edwards, 1861), the pea crab Pinnixa rathbuni (Sakai, 1934), and the porcelain crab Pachycheles stevensii (Stimpson, 1858). These species belonged to six families: Lithodidae, Atelecyclidae, Majidae, Portunidae, Porcellanidae, and Pinnotheridae. The role of the variability of some of the hydrological factors in the meroplankton larvae development was studied. In May, the crab larvae were found mostly in the open part of the bay. In June, they were transported by the current to the northern part of the bay. In July, they spread uniformly in all the bay areas; however, patchiness was observed. The maximal population density of the crab larvae was registered for July and varied from 6.8 to 23.3 ind. m⁻³. The crab larvae appeared in the plankton in 2000 and 2001 earlier than for the average season.     

锯缘青蟹(Scylla serrata)幼体胃磨发育的比较研究

在青蟹幼体发育过程中，胃磨呈现出明显的变化。Ｚ１缺乏胃磨的结构，ＺⅡ开始出现中齿和侧齿的雏形，明显的可以识别的中齿和侧齿出现在ＺⅢ，．从ＺＶ开始胃磨已臻完善，除具有中齿和侧齿外，还出现了４个附属齿，包括两个附属的中齿和两个附属的侧齿。大眼幼体期，中齿和侧齿比ＺＶ更加尖锐，附属齿也更加锋利。在仔蟹前，中齿齿尖发生特化，形成垫状角质化区域，侧齿、附属齿更加锋利。这表明青蟹幼体发育过程中胃磨结构的变化与     

Bacteriological investigation of the neuston and plankton in the Fraser River estuary,British Columbia

Microbiological parameters were examined in the surface microlayer (150 μm) and the subsurface waters (1 m) of the Fraser River estuary (British Columbia) at salinities of 0, 15 and 25‰. Little difference was observed in the neustonic variables between these three stations. A comparison of the neuston with the plankton over the estuary as a whole revealed elevated detritus, particulate organic carbon and nitrogen but reduced ATP, heterotrophic activity and percentage respiration in the neuston. Enrichment of bacterial numbers or dissolved organic carbon was not observed in the surface microlayer.     

Natural history of the pea crab in Wellington Harbour,New Zealand

The pea crab Pinnotheres novaezelandiae Filhol (Brachyura: Pinnotheridac) infests the mussel Perna canaliculus (Gmelin) in Wellington Harbour. The crab passes through the same post‐planktonic stages as other pea crabs. The number of eggs hatching increases over summer, although females can be found incubating eggs at any time of the year. Shellfish below the level of low water neap tide are more heavily infested than those above this level. Damage to the host is restricted to erosion of the demibranchs and nodule formation on the mantle.     

Retention of crab larvae in a coastal null zone

Alongshelf transport in the southern Middle Atlantic Bight is forced by buoyancy-driven currents originating in three large estuaries along the bight. These currents are strongest in the coastal ocean near the southern terminus of each estuary, while the analogous region on the northern side is characterized by weak subtidal flow. We used a combination of field observations and numerical modeling to test the hypothesis that these regions of weak subtidal flow are coastal null zones that serve as retention areas for larvae. The field study consisted of a four-day, shipboard investigation of the distribution of blue crab larvae (Callinectes sapidus) near the mouth of Delaware Bay (39°N, 75°W) in late summer, 2004. Hydrographic surveys of the study site were conducted with a hull-mounted, surface-measuring system. Results showed a sharp boundary between the null zone and the buoyancy-driven current to the south. Blue crab larvae were collected in surface plankton tows along a 30-km transect that encompassed these two areas. Stations with higher densities of larvae were clustered in the null zone during both ebb and flood tides. A numerical model was used to examine the physical mechanisms responsible for the observed distribution. Model results agreed with the field survey and showed that simulated larvae are aggregated in the null zone. The simulations also demonstrated that larvae spawned within the null zone have a much greater probability of settling in juvenile nursery habitat within the bay. The close agreement between field and model results provides consistent support for the hypothesis that coastal null zones associated with the buoyancy-driven circulation of large estuaries may allow retention of larvae in the vicinity of the natal spawning population.     

青岛沧口潮间带小型底栖生物的时空分布研究

于2008年7、10月,2009年1、4月对青岛沧口潮间带进行了小型底栖生物调查。结果表明,小型底栖生物的年平均丰度为(936.02±565.31)ind.10cm-2,平均生物量为(561.89±322.57)μg.10cm-2(干重)。共鉴定出10个类群,线虫在丰度上占绝对优势(95.41%),按生物量,线虫占63.58%,多毛类居次,占13.21%。丰度有潮区差异,生物量呈现季节和潮区差异。在垂直分布上,表层0～4层分布最多(42.57%),向深层呈现递减趋势,并且垂直分布表现出季节变化。高潮带春季表层的小型底栖动物数量百分比最高,冬季表层的数量百分比显著的低于其他3个季节。相反,中潮带冬季表层数量百分比却明显高于其他3个季节。Pearson相关分析未表明小型底栖生物总丰度和环境因子的显著相关。但对各层的小型底栖生物丰度和环境因子的Pearson相关分析表明,0～4cm的小型底栖生物丰度和叶绿素及中值粒径显著相关,8～12cm的小型底栖生物丰度和中值粒径显著相关。BIOENV分析表明,能够解释小型底栖动物空间分布的的最佳环境因子组合为有机质、中值粒径和含沙量。     

中华豆蟹对青岛海区贻贝的感染及危害初探

20 0 0年 10月～ 2 0 0 1年 12月 ,对青岛太平角养殖海区的贻贝寄生中华豆蟹的感染情况进行了调查。以肥满指数为指标 ,通过对感染和未感染贻贝的比较 ,分析了中华豆蟹对贻贝的危害程度。结果表明 ,中华豆蟹对贻贝的感染率为 2 .0 %～ 2 0 .4 %。2 0 0 0年 11月和 2 0 0 1年 11月出现 2次感染高峰 ,分别为 9.7%和 2 0 .4 % ;最低感染率出现于 2 0 0 1年 1月和 3月 (均为 2 .0 % )。感染强度为 1～ 5 ,多数为 1(占感染个体的 83.2 % )。贻贝所感染的豆蟹的性别及感染强度与宿主的大小有关。小个体贻贝仅见雄豆蟹感染 ,绝大多数雌豆蟹见于壳长大于 2 cm的贻贝。未发现同一宿主体内同时感染 2只或以上的雌豆蟹。在调查的 15个月中 ,有 11个月被感染贻贝的肥满度显著低于未感染的贻贝 ,且雌豆蟹对宿主肥满度的影响大于雄豆蟹