Effects of γ-Rays on Hatching' of the Eggs and Survival Period of Fries of Cyprinus carpio

The eggs of Cyprinus carpio were irradiated by 5 different strengths: 0.0258, 0.129, 0.258, 0.516 and 1.29 C/kg (100, 500, 1000, 2000 and 5000 R) of γ-rays in the batches of 50 fertilized eggs. The activities of eggs and hatched fries were noted after irradiation doses. The hatching percentages of the eggs were reported for each group. The percentage of hatched eggs of Cyprinus carpio after radiation doses of 0.0258, 0.129, 0.258, 0.516 and 1.29 C/kg (100, 500, 1000, 2000 and 5000 R) was 30%, 24%, 28%, 30%, and 34%, respectively. The 50% mortality period of fries for the above strengths of the γ-rays was 8 days, 7 days, 6 days. 4 days and 4 days, respectively. The survival period of the fries was 11 days, 9 days, 8 days, 7 days and 6 days, respectively. All fries hatched from the eggs irradiated by γ-rays showed early hatching, a higher percentage of mortality, a retarded and abnormal development of the body. A statistical analysis of the results by the correlation coefficient also proved that the hatching percentages of the fries and the survival period of fries are directly proportional to the strength of the γ-rays and inversely proportional to the strength of γ-rays, respectively.     

Fenvalerate and Cypermethrin Induced Changes in the Haematological Parameters of Cyprinus carpio

Freshwater fish Cyprinus carpio have been exposed to sublethal concentrations of fenvalerate (0.010 ppm) and cypermethrin (0.020 ppm) for 6, 12, 24 and 48 hours. After each exposure period, certain blood parameters were studied. A decrease in red blood corpuscular (RBC) count, haemoglobin (Hb) concentration, packed cell volume (PCV) and mean corpuscular haemoglobin concentration (MCHC), on the one hand, and an increase in mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH), on the other hand, have been observed with increasing time of exposure. The alterations observed may be due to the physiological disfunctioning of the haemopoietic tissue or may be due to the effect on the bone marrow. The effect of fenvalerate is more pronounced than that of cypermethrin.     

Determination of the Maximum Acceptable Toxicant Concentration (MATC) and the safe Concentration for Certain Aquatic Pollutants

— The 96 h TL₅₀ values, the maximum acceptable toxicant concentration (MATC) and the safe concentrations were determined for various aquatic pollutants, viz. thiotox, chlordane, metasystox, dichlorvos, sevin and carbofuran (pesticides), phenol, pentachlorophenol and dinitrophenol (phenolic compounds), cadmium chloride and copper sulphate (heavy metal), swascofix P₁₄, swanic 6 L and swascol 1P (synthetic detergent) using the larvae (the most sensitive stage of the life cycle) of Cyprinus carpio, a fresh water exotic carp. — The estimated MATC ranged between 0.009 and 400 μg/1 for all the pollutants; — the calculated value of the application factor (AF) ranged between 0.0096 and 0.0978. — The safe concentrations were determined for each pollutant by the lowest value of application factor worked out in the present investigation; — safe concentrations were also worked out by different formulae given by different workers. The values obtained were compared with the safe concentrations value worked out by the authors of the present study.     

Studies on the Effect of DDT on the Regeneration of Cutaneous Wounds in Mystus (mystus) vittatus Bl. (Bagridae)

The effects of DDT on the regeneration of cutaneous wounds has been studied in Mystus (mystus) vittatus. The fishes were locally collected, they were inflicted incised wounds surgically and kept in 0.01 and 0.15 ppm concentrations of DDT. The regenerating skin at different stages of development ranging from 15 min to 25 d after the injury was studied through microtomy sections. The following toxic effects were studied:

• A small amount of DDT accumulated in the subcutis at the 25 days stage,
• the regeneration of wounds under DDT stress has been slower, delayed or irregular,
• the secretion of fibrinous exudate has been copious,
• epithelization and fibroblastic activities have been slower,
• the higher concentration of 0.15 ppm of DDT produced more toxic effects than the lower concentration of 0.01 ppm,
• the earlier stages of wound healing up to 3 d have been more adversely affected than later stages.

     

Hexavalent chromium: Toxicity and impact on carbohydrate metabolism and haematological parameters of carp (Cyprinus carpio L.) from Saudi Arabia

The effect of hexavalent chromium on the carp,Cyprinus carpio was assessed using static bioassay. The 96h LC₅₀ was found to be 93.6mg · l^–1. In carp exposed to sublethal concentrations of chromium (15 and 25 mg · l^–1), significant depletion of liver and muscle glycogen and decreasing leucocyte counts were recorded during prolonged exposure and with increasing concentration of chromium. The other blood parameters examined (haemoglobin, erythrocyte count, hematocrit, serum protein, serum glucose) increased similarly during exposure to two chromium concentrations.     

汉江中游鱼类资源现状

2003-2004年在汉江中游江段共监测654船次,统计渔获物6914.16 kg,生物学测定1682尾鱼,共采集鱼类78种,隶属18科58属.目前汉江中游渔获物组成和结构与20世纪70年代相比发生了较大变化,翘嘴红鲌、瓦氏黄颡鱼、鲶、鲢、黄尾鲴、大鳍蠖等在渔获物中比例已经很少;草鱼资源也明显下降;铜鱼、青鱼、蒙古红鲐、鳡、细鳞斜颌鲴、吻鮈、长吻鮠、拟尖头红鲌、鳙、鳜等在渔获物中已基本消失;而鲤、鲫、黄颡鱼、长春鳊、赤服鳟等中小型鱼类在渔获物中的比例却相对上升;渔获物中个体大的鱼减少,低龄鱼及幼鱼个体比重增加.与70年代资料相比,汉江中游鱼类资源已呈衰退趋势.     

Studies on the Pollution of the Kalinadi by Industrial Wastes near Mansurpur Part 2: Biological Index of Pollution and Biological Characteristics of the River

From the biological data presented here it is difficult to define any species of fish as an indicator of pollution in the strict sense. Nevertheless, the following generalizations are possible.

• - Species as Wallago attu, Clarias batrachus, Heteropneustes fossilis, Ophiocephalus striatus and Oph. punctatus occur in good number in the polluted stretch and seem to be tolerant species.
• - The species as Esomus danrica, Labeo rohita, Labeo gonius, L. bata, Catla catla, Cirrhina mrigala, Barbus chagunio, Barbus spec., Notopterus notopterus and Mugil corsula are most sensitive and disappear first in the polluted stretch.
• - The species as Barilius bola, B. bendelisis, Nemacheilus latius, Notopterus chitala, Collichrous bimaculatus, and Nandus nandus are moderately sensitive.
• - It appears that the main damage is caused to carp and feather-back fishes. The whole stretch of heavy pollution is almost devoid of carp and featherback.

The following important conclusions can be drawn from the distribution of plankton and bottom organisms:

• - The higher number of protozoans, chironomids, maggots and the decrease in the number of mollusca can be considered as the indicator of pollution, because of their abundance in polluted zones.
• - Chlorella, Lyngbya, Gyrosigma and Euglena (algae), Viviparius (mollusc) and Bosmina (crustacea) are very sensitive to pollution.
• - Oscillatoria, Pandorina, Anabaena, Nitzschia, Closterium (algae), Branchionus, Rotatoria (rotifers) and Naidium (annelid) are moderately sensitive.
• - Synedra, Spirogyra, Desmidium, Rivularia, Navicula, Fragillaria Phacus, Arcella and Vorticella are fairly tolerant forms.
• The stretch studied has been subdivided into five zones with characteristic features:
• - The Oligosaprobic Zone - characterized by a sufficient oxygen saturation, low B. O. D. and C. O. D., with a good fauna and flora.
• - The Polysaprobic Zone - with low oxygen saturation, high B. O. D. and C. O. D. and hydrogen sulphides with a restricted fauna and flora.
• - The α-Mesosaprobic zone - with moderate oxygen saturation, moderate B. O. D. and C. O. D. traces of hydrogen sulphide with the improvement of the fauna and flora.
• - The β-Mesosaprobic Zone - with fair oxygen saturation, low B. O. D. and C. O. D., with the reappearence of fauna and flora.
• - The Oligosaprobic Zone - similar to the zone of low pollution in all characteristics.

     

“Man and the Biosphere”-Studies on Sikkim Himalayas. Part 1: Acute Toxicity of Copper and Zinc to Common Carp Cyprinus carpio (Linn.) in Soft Water

In the daily exchange of the medium with only 7.1 mg/l Ca²⁺ and 0.7 mg/l Mg²⁺ during the batch experiment the LC_50,96h for Cyprinus carpio is 0.063 mg/l Cu²⁺ and 3.12 mg/l Zn²⁺ at water temperatures of 11 to 14°C. Significant differences in mortality as referred to the LC_50,12h occur for copper only after 72 h of exposure and for zinc after 48 h of exposure. The values of LC_50,96h are clearly lower for both metals in the investigations described than most data given in literature for different fish species.     

“Man and Biosphere” — Studies on Sikkim Himalayas. Part 2: Acute Toxity of Mixed Copper-Zinc Solutions on Common Carp,Cyprinus carpio (LINN.)

In batch experiments for 6 to 48 h, individuals of Cyprinus carpio (2 g individual weight) were exposed to mixed solutions of copper and zinc sulphate: pH = 6.3, temperature 15 °C, 6.8 mg/l O₂, 7.1 mg/l Ca²⁺, 0.7 mg/l Mg²⁺. The LC₅₀ for the individual substances on exposures of 24 and 48 h amount to 9.04 and 7.28 mg/l, resp., of Zn and 0.11 and 0.095 mg/l, resp., of Cu. The sum of the biological activity of the two kinds of metal ions is assessed according to MARKING'S index: at low concentrations of the mixed solutions the two metals show an additive effect, at higher concentrations a synergistic effect occurs.     

Acute Toxicity of Some Industrial Effluents in Relation to the Fish Size of Cyprinus carpio (HAM.) and Channa punctatus (BLOCH)

The fish Cyprinus carpio (HAM.) and Channa punctatus (BL.) of the size ranging between 6.0 ± 1.2 cm and 28.0 ± 1.6 cm were exposed to different concentrations of industrial effluents from M/s Punjab Tannery Ltd. (Jalandhar), M/s Spinning Mill Ltd. (Hoshiarpur) and M/s Food Specialist Ltd. (Moga) for evaluating the influence of fish size on the acute toxicity of these effluents. Results indicate that the relative toxicity of the effluents decreased with the increase in fish size from 6.0 ± 1.2 cm to 20.6 ± 1.5 cm. However, for specimens of the size of 28.0 ± 1.6 cm and more, the toxicity of the effluents increased with an increase in fish size. On the basis of the relative toxicity to fish, effluent from Punjab Tannery was found highly toxic and from Food Specialist Industry, the least toxic.     

太湖常见鱼类生态学特点和增殖措施探讨

对太湖55种常见鱼类的生态学特点和种群动态等作了较全面的综合分析, 认为:鱼类的生态学特点和环境因子（包括人为因子）的综合作用是造成目前太湖在鱼类组成、种群数量上均以小型鱼类占绝对优势, 大、中型经济鱼类种群数量下降、结构低龄化的主要原因。因此, 太湖增值措施应包括:限制刀鲚发展、扶持翘嘴红鲌、鳜等名贵凶猛鱼类、加强人工放流、重点保护草上和水底部产卵鱼类、提高起捕规格等。     

The Accumulation of Monocrotophos in Some Piscine Organs

Monocrotophos (0,0-dimethyl-0-(1-methyl-2-N-methyl-carbamoyl)-cis-vinylphosphate) as an active component of some insecticides is highly soluble in water at a low hydrolysis rate: half-time at 2 mg/l, pH = 7.0 and 38 °C 23 d. Individuals of the following fish species (individual weight in g) were kept during the static bioassay for 6… 72 h at 40 mg/l monocrotophos in the medium: Cyprinus carpio (70), Carassius carassius (45) and Leucaspius delineatus (1.5), and then the concentration of monocrotophos was gas-chromatographically determined in different tissues. Initially, till the 24th hour a high accumulation in the gills took place (in mg/kg fresh weight: Cyprinus 0.49, Carassius 3.88), then the concentration remained uniform or slightly falling here, whereas in the other organs (digestive organs, muscle, gonads) a further accumulation up to 1 mg/kg occurred. Leucaspius was investigated only as whole individuals, and after 72 h their fresh weight had increased to 6.96 mg/kg. The rates of accumulation decrease in the following order: Cyprinus: digestive organs, gills, muscle; Carassius: gills, digestive organs, gonads, muscle.     

Biocides in Relation to Water Pollution Part I: Bioassay Studies on the Effects of a few Biocides on Fresh Water Fish,Channa gachua

• - In the present paper the toxicity of four biocides, endrin, thiodon, malathion and phosvel, has been measured by performing static bioassay experiments with Channa gachua as test fish to establish the median Tolerance Limits (TL_m and TL₅₀).
• - The results of bioassay experiments indicate that the fish is harmed even at a very low concentration of these biocides. The values of TL_m are 0.0076, 0.0073, 0.00695 and 0.0068 for endrin; 0.01120, 0.01088, 0.01069 and 0.01064 for thiodon; 9.20, 8.10, 7.90 and 7.60 for malathion and 32.90, 32.10, 31.65 and 31.15 mg/l for phosvel for the time interval of 24, 48, 76 and 96 hours, respectively.
• - As indicated by TL_m's, endrin is most toxic and phosvel least. Thiodon and malathion came in a descending order between endrin and phosvel.
• - During bioassay studies, the effect of some variables, like temerature, pH and fish-size, on TL_m's has also been observed. They gave the following results.
• a) An increase in temperature increased the toxicity of all the biocides.
• b) An increase in pH decreased the TL_m as such, making the biocides more toxic to Channa gachua.
• c) For larger fishes, the biocide solutions were less toxic, but the same solutions were more toxic to fishes of a smaller size.
• - The safe harmless concentrations of endrin, thiodon, malathion and phosvel are - 0.00289 mg/l, 0.00337 mg/l, 1.88365 mg/l and 9.16736 mg/l, respectively.

     

呼伦湖鱼类群落结构及其渔业资源变化

根据2014-2015年在呼伦湖进行的鱼类资源拖网调查,结合历年渔业捕捞资料,分析该水域的鱼类群落结构及渔业资源发展趋势.结果表明:本次调查共采获鱼类21种,隶属4目6科21属,其中鲤形目种类最多,占总数的81.0%;群落优势种为贝氏(Hemiculter bleekeri)、红鳍原鲌(Cultrichthys erythropterus)、鲤(Cyprinus carpio)、银鲫(Carassius auratus gibelio)和瓦氏雅罗鱼(Leuciscus waleckii),鱼类群落的丰富度指数、Shannon-Wiener多样性指数和均匀度指数均偏低.东部、西部湖区间的鱼类群落组成存在一定差异,其中西部湖区的鱼类物种数量较少,但大中型鱼类比例及生物多样性较高.与历史资料相比,呼伦湖鱼类的物种数量下降,优势种组成发生较大变化,鱼类个体小型化趋势明显,而捕捞强度过大、湖泊富营养化加剧及气候水文条件变化是导致呼伦湖渔业资源衰退的主要因素.     

Studies on the Toxicity of a few Pesticides on Colisa fasciatus and Notopterus notopterus with Reference to Histopathology and Histochemistry. Part 1: Bioassay Studies

An attempt has been made to study the toxicity of two commonly used pesticides aldrin (organochlorine) and ethyl-parathion (organophosphorus) to the fish Colisa fasciatus (Anabantidae) and Notopterus notopterus (Notopteridae). During bioassay studies, the behaviour of the fishes was recorded. The aldrin concentration used lies between 0.021 mg/1 and 0.042 mg/1 for C. fasciatus and 0.00056 mg/1 and 0.00135 mg/1 for N. notopterus. The ethyl-parathion concentration used lies between 1.8 mg/1 and 3.7 mg/1 for C. fasciatus and 0.49 mg/1 and 1.00 mg/1 for N. notopterus.

• – The effect of lethal aqueous concentrations of pesticides on the Fishes prior to death are usually manifested by excitation, erratic swimming ability, difficulty in respiration, increasing in ventilation rate, jerky movements of body and fin fast, up- and downward movements, convulsions, loss of equilibrium, loss of the original colour of the body skin and the excess secretion of mucus by the gills and the body wall.
• – the TL_m values for 24, 48 and 96 hours of exposition are determined for both species of fish and both pesticides at the following variables:

• ? three levels of temperature,
• ? three levels of dissolved oxygen,
• ? three levels of pH,
• ? three fish sizes.

• – statistical analysis of variance shows, that all variables have a significant effect on the TL_m values of both pesticides for both fish species;
• – Aldrin (chlorinated hydrocarbon) is more toxic than ethylparathion (organophosphate) at all variables;
• – Colisa fasciatus is the more resistant species than Notopterus notopterus.
• – the relationship between the total number of the fishes N₀, survival numbers N and time t can be expressed by a mathematical formula as:
• – the harmless concentration to C. fasciatus ranged from 0.0049 mg/1 to 0.0161 for aldrin, 0.54 mg/1 to 0.99 mg/1 for ethyl-parathion and to N. notopterus from 0.00012 mg/1 to 0.00045 mg/1 for aldrin, 0.10 mg/1 to 0.21 mg/1 for ethyl-parathion;
• – the aldrin should be diluted at least 4000 times and ethyl-parathion 320 times of its actual concentration.

     

基于微卫星标记的长江江苏段鲢(Hypophthalmichthys molitrix)增殖放流资源贡献率的评估

近年来,长江下游进行了大规模鲢(Hypophthalmichthys molitrix)增殖放流活动,而增殖放流对鲢资源的贡献如何,成为亟待探究的科学问题.本研究基于已开发的11对微卫星引物,标记8个主要原良种场(为江苏省各地政府放流工作提供苗种的单位)鲢繁殖亲本群体,利用微卫星亲子鉴定技术,评估2016-2017年长江江苏段鲢增殖放流对鲢在江群体的资源贡献率.研究结果显示,621尾繁殖亲本群体和475尾长江捕捞鲢群体共检测出191种等位基因,每个座位的等位基因种类数、观测杂合度、期望杂合度范围分别为9～36、0.536～0.793、0.597～0.941;对应平均值分别为17.36、0.656、0.794.11个座位均表现出高度的多态性,每个座位的多态信息含量范围为0.560～0.938,平均值为0.771,证明选用的11对微卫星引物可作为亲子鉴定的有效工具.亲子鉴定结果表明,在亲本性别未知的情况下,非亲排除率(NEP)范围为2.4%～42.4%,累积非亲权排除率(CEP)在99.99%以上; 475尾长江鲢群体中,鉴定出39尾鲢个体来自于原良种场鲢亲本群体所繁殖的后代,即回捕率为8.21%.综合分析可见,2016-2017年,长江江苏段增殖放流对鲢资源量有较好的补充作用.     

基于水声学探测的镜泊湖鱼类时空分布特征及资源量评估

2020年5、7和10月,采用多网目复合刺网调查和水声学探测相结合的方式对镜泊湖鱼类群落时空动态和资源量进行调查．渔获物调查共采集鱼类4目6科29种,其中鲤科鱼类最多,主要经济鱼类为鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)、蒙古鲌(Chanodichthys mongolicus)、瓦氏雅罗鱼(Leuciscus waleckii)、鲤(Cyprinus carpio)、翘嘴鲌(Culter alburnus)．蒙古鲌和■(Hemiculter leucisculus)为优势种．水声学探测结果表明,镜泊湖鱼类密度在春季、夏季和秋季分别为836、7216和2724 ind．/hm²,其中夏季鱼类密度最高且主要集中于中部和南部区域,春季和秋季鱼类密度则呈现从南至北递减的趋势．春、夏、秋季鱼类的平均目标强度为-43.4、-50.0和-46.5 dB,估算的平均全长分别为34.0、18.0和25.2 cm,推算的平均体重分别为272.7、43.7和135.2 g．结合各季节平均密度,估算出春、夏、秋季镜泊...     

Size related toxicity of industrial effluents to cyprinus carpio (HAM.) and Channa punctatus (BL)

Fish, Cyprinus carpio and Channa punctatus in four sizes (6.0 ± 1.2; 12.5 ± 1.6; 20.6 ± 1.5 and 28.0 ± 1.8 cm in total length) were exposed to industrial effluents from the Sangam Dyeing Factory (Ludhiana), the Sterling Steel and ABC Paper Mills (Hoshiarpur) to evaluate the acute toxicity of these effluents at different fish sizes. Results reveal that the toxicity of industrial effluents decreased with the increased fish size from 6.0 ± 1.2 to 20.6 ± 1.5 cm in length. However, for specimens of the size of 28.0 ± 1.6 cm and more, the toxicity of the effluents increased with an increase in fish size. On the basis of the relative toxicity to fish, effluent from the Sterling Steel Mill was found to be highly toxic to fish and effluent from the Dyeing Factory least toxic except for the fish of 6.0 ± 1.2 cm at which dyeing factory effluent was most toxic and ABC paper mill effluent the least toxic.     

DEVELOPMENT OF MORE EFFICIENT AIRGUN ARRAYS: THEORY AND EXPERIMENT*

Source strength of an airgun array may be increased by:

• — utilizing higher pressure,
• — increasing total array volume,
• — employing more guns,
• — improving gun efficiency.

One measure of gun efficiency is “specific source strength”, P_a*, defined as source strength per unit quantity of air used. Typical units are MPa m/l. Most developments are directed toward increasing gun pressure and/or gun volume to increase source strength of the array. These efforts require that more air compressors be installed onboard the ship. Consequently, a larger ship may be needed for the additional compressors, guns, and auxiliary equipment. A development program was initiated in 1976 to increase source strength of the array without using a larger ship. New guns were designed and built—one for 41.4 MPa and 7.37 liter (6000 p.s.i./450 in³) operation and another with 13.8 MPa and 4.92 liter (2000 p.s.i./300 in³) capability. Experiments were conducted with these new guns (and existing guns) over a range of pressures from 13.8 to 41.4 MPa (2000 to 6000 p.s.i.). Design of the new guns was aided by a mathematical model. The model relates physical dimensions of the airgun to acoustic pressure in the water. It consists of four nonlinear differential equations relating

• — shuttle motion,
• — bubble pressure,
• — chamber pressure,
• — bubble radius.

The last equation is the “free-bubble-oscillation equation” and represents the ideal case of a pressurized bubble released instantaneously in water. The three other equations modify this ideal case; the four equations together model an airgun of the type manufactured by Bolt Associates, Inc.