The occurrence of an exotic bisexual Artemia species, Artemia franciscana , in two coastal salterns of Shandong Province, China

The alien halophilous Artemia species, Artemia franciscana, was found in Chengkou Saltern and Yangkou Saltern of Shandong Province, P.R. China. Although the indigenous parthenogenetic Artemia is detectable, the exotic species is dominant in both salterns. The cross-breeding tests between the exotic A. franciscana and 5 bisexual Artemia species were conducted. The results of hybridization and morphological observations on the exotic A. franciscana are briefly presented in this short communication.     

Impact of Colonization of an Invasive Species on Genetic Differentiation in New Environments: A Study on American Artemia franciscana(Crustacea: Anostraca) in the United Arab Emirates

Artemia franciscana, native to America, has recently colonized as non-indigenous population in Asia, Europe, North Africa, and Australia. We evaluated the effects of the colonization of A. franciscana on genetic differentiation in new environments in the United Arab Emirates(UAE). We used the COI marker to determine the genetic structure and origins of exotic populations in the UAE. Results confirmed the colonization of A. franciscana in two localities. Invasive populations of A. franciscana had significantly lower genetic variation than native populations in the Great Salt Lake and San Francisco Bay. Results showed that the studied populations could not have colonized directly from natural American habitats, and they possibly were from secondary introduction events of other non-indigenous populations. Genetic analysis yielded different demographic patterns for the studied invasive populations. The population in Al Wathba Wetland Reserve(AWWR) demonstrated demographic expansion, whereas in Godolphin Lakes(GL), it reached a demographic equilibrium. Neutrality tests showed an excess of recent and historical mutations in the COI gene pool of invasive AWWR Artemia in the new environment. The results suggest that different ecological conditions in new environments can exert selective pressures during the introduction of an exotic population, which can affect genetic variation.     

Impact of brine acidification on hatchability,survival and reproduction of Artemia parthenogenetica and Artemia franciscana in salt ponds,Bohai Bay,China

We studied the effect of pH(pH 5, 6, 7 and 8) on the hatching percentage, survival and reproduction of Artemia strains in Bohai Bay salt ponds. Strains included parthenogenetic Artemia from Bohai Bay(BHB), Artemia franciscana from San Francisco Bay, and A. franciscana artifi cially produced in salt ponds in Vietnam. The latter was included as a potential inoculum for biological management of salt ponds. The hatching percentage of cysts after 24 h and the survival rate of the tested Artemia strains were signifi cantly reduced when exposed to a culture medium at pH 5 for 18 d( P 0.05). The tolerance of Artemia to 48 h acid exposure varied with developmental stage, increasing in the following order: juvenile, nauplii, pre-adult, with maximum tolerance in adults. All strains of Artemia tested could not reproduce at pH 5. At pH levels from pH 6–8, a higher pH generally resulted in a shorter brood interval and enhanced ovoviviparity. Hence, we suggest that brine acidifi cation has a negative impact on Artemia populations in the Bohai Bay saltworks. Inoculation of Artemia with either local parthenogenetic Artemia or exotic A. franciscana should be feasible at pH 7–8.     

Effect of carbon and nitrogen ratio control on Artemia growth,water quality,biofloc microbial diversity under high salinity and zero-water exchange culture condition

Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species.The growth of heterotrophic microorganisms can be stimulated through adding extra carbon,which reduces the nitrogen level in the water and provides microbial protein to the animals.However,most of the studies and practical applications have been conducted in freshwater and marine environment.This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms.The effect of carbon supplementation on Artemia growth,water quality,and microbial diversity of bioflocs was studied in the closed culture condition without any water exchange.The salinity of the culture medium was 100.A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen(C/N)ratio of 5,15,and 30(Su5,Sul5,and Su30),respectively.The culture without adding sucrose was used as a control.Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level.The results showed that sucrose supplementation at higher C/N ratio(15 and 30)significantly improved the Artemia survival,growth and water quality(P0.05).Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume(BFV)(P0.05).The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness,and shaped the microbial composition at genera level.This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.     

Proteomic analysis of acute responses to copper sulfate stress in larvae of the brine shrimp, Artemia sinica

Proteomics was used to reveal the differential protein expression profiles of acute responses to copper sulfate exposure in larvae of Artemia sinica. Fourteen differentially displayed protein spots were detected and seven of them were identified. Three spots were up-expressed and identified: actin, heat shock protein 70, and chaperone subunit 1; three down-regulated proteins were identified: arginine kinase, elongation factor-2, and glycine-rich protein; and a newly expressed protein was identified as peroxiredoxin. The study indicates the involvement of all the differentially expressed proteins in the early responses of protein expression, and in the survival of A. sinica in the presence of copper and other heavy metals; the findings improve understanding of the organism’s adaptive responses and resistance.     

Effect of n-3 HUFA levels in rotifers and Artemia on growth and survival of larval black sea bream （Sparus macrocephalus）

Requirement for dietary n-3 HUFA (n-3 highly unsaturated fatty acid) for growth and survival of black sea bream (Sparus macrocephalus) larvae was studied using rotifers and Artemia at various levels of n-3 HUFA. Five treatments with rotifers and Artemia differing in n-3 HUFA were prepared by enriching them with various oil emulsions. Results indicated that dietary n-3 HUFA significantly influence fish n-3 HUFA levels and are essential for growth and survival of black sea bream larvae. The results also indicated that the incorporation of n-3HUFA TG (triacylglycerols) into tissues of larval black sea bream was more effective from natural fish oil in comparison with n-3 HUFA fatty acid ethyl esters from ethyl-esterified oil.     

不同饵料强化剂对龙虎斑仔稚鱼生长、存活率、消化酶活力及体成分的影响

褶皱臂尾轮虫(Brachionus plicatilis)与卤虫(Artemia franciscana)无节幼体分别以浓缩小球藻、深海鱼油和鱼肝油强化后,投喂龙虎斑(Epinephelus fuscoguttatus♀×E.lanceolatus♂)仔稚鱼,研究不同强化剂对仔稚鱼生长、存活率、消化酶活力及体成分的影响。结果显示,3种强化剂强化后的生物饵料均可显著提高龙虎斑仔稚鱼的存活率、体长和体质量,差异有统计学意义(P0.05),但对鱼体粗脂肪含量以及肠淀粉酶活力的影响无统计学意义(P0.05)。深海鱼油组仔稚鱼存活率最高,浓缩小球藻可以显著提高胃、肠蛋白酶活性和鱼体粗蛋白含量,各组差异有统计学意义(P0.05),而深海鱼油和鱼肝油可以显著提高肠脂肪酶活性(P0.05)。结果表明,3种强化剂各具优点,均可用于龙虎斑仔稚鱼生物饵料的强化,尤其以深海鱼油提高存活率最显著。     

The brine shrimp (Artemia parthenogenetica) as encapsulation organism for prophylactic chemotherapy of fish and prawn

Brine shrimp (Artemia parthenogenetica) which had ingested three water-insoluble antibacterial drugs i.e. sulfadiazine(SD), oxytetracycline (OTC) and erythromycin estolate (ERY-Es) were fed toTilapia and Mysis IIIof Penaeus orientalis K. The drug contents in the predators were then determined. After administration of drugs toTilapia and Mysis III, through the bio-encapsulation of the brine shrimp, efficacious therapeutical concentration of OTC and ERY-Es (but not SD) in the predators could be reached and maintained for more than 8 hours.     

Assessing the invasion risk of botanical garden's exotic threatened collections to adjacent mountain forests: A case study of Cibodas Botanical Garden

A primary objective of botanical gardens is to conserve threatened plant species from different regions and countries. However, such ex-situ conservation practices for exotic plant species may pose a significant plant invasion risks. In this study, we predict the naturalization probability of exotic threatened plant species of Cibodas Botanical Garden(CBG) collections based on leaf trait(specific leaf area, SLA) or as a function of invasion risk assessment scoring system(Tropical Weed Risk Assessment Protocol, TWRAP). We found that SLA and TWRAP were positively correlated with naturalization probability. The TWRAP model produced higher predictive probabilities with larger uncertainty compared to SLA model. Parmentiera cereifera and Burretiodendron hsienmu are two species that have highest naturalization probability based on SLA model. Chamaedorea oblongata has the highest naturalization probability based on TWRAP model. From practical and management point of view, we recommend the simultaneous use of SLA-based and TWRAP-based invasive species risk assessment to estimate the naturalization risk of exotic threatened collections of botanical gardens to adjacent mountain forests. Finally, given the important conservation value of threatened exotic collection of botanical garden, we need to be aware with the invasion risks of these species. The threatened condition of a plant species is not only caused by its natural characteristics but also may be caused(in fact mostly confounding with) by external disturbance either natural catastrophic or human related activities. Thus, a threatened species may not necessarily a noninvasive species outside their natural distribution ranges.     

Isoenzymes and soluble protein in Arthrospirafrom alkaline lakes in Erdos Plateau, China and in exotic species

1 INTRODUCTION Since isoenzyme was first named by Markert and M?ller in 1957 (Hu and Wan, 1985), the separa- tion of isoenzyme has been rapidly developed. Isoenzymes is an important tool for studying life in molecule level. Isoenzymes are transcribed by g…     

Dormant stages of crustaceans as a mechanism of propagation in the extreme and unpredictable environment in the Crimean hypersaline lakes

A pool of dormant stages of planktonic organisms in saline lakes is a substantial component in the plankton communities;we need to take it into account to understand plankton dynamics.Hypersaline water bodies in Crimea,the largest peninsula in the Black Sea,constitute a very characteristic and peculiar habitat type in the region.We examined the presence of crustacean resting stages in sediments of dried up sites of the Crimean hypersaline lakes.Sediment samples were taken in 9 different lakes.Experiments performed on the hatching of these resting stages showed the presence of Moina salina(Cladocera),parthenogenetic Artemia and Artemia urmiana(Anostraca),Eucypris mareotica( inflata)(Ostracoda),and Cletocamptus retrogressus(Harpacticoida).Comparing the experimental results obtained with clean dried brine shrimp cysts and those kept in sediment samples,it was noted that clean cysts hatched much faster than those from sediments did.Some components in bottom sediments slow down and desynchronize hatching from resting eggs in different groups of crustaceans.The sediments of different lakes inhibited the nauplii output from Artemia and ostracod resting eggs to different degrees.More data are needed before we can discuss the reasons of this inhibition.The nonsynchronous output of active stages from the bottom resting ones may be an adaptation that allows crustacean species to exist in extreme and unpredictably changing environments,avoiding the risk that all may emerge at once under unsuitable conditions.     

Leaf thickness and elevation explain naturalized alien species richness in a tropical mountain forest: A case study from Mount Gede-Pangrango National Park,Indonesia

Exotic plant invasion is one of the major causes of species extinction. In many contexts, mountainous forests are the last refuge for native species. There are several inventory studies conducted in mountainous and tropical forests in Indonesia. However, there are no studies yet on the factors that explain the abundance and richness of surveyed naturalized alien species. This study investigated whether the number of individuals and abundanceweighted species richness(AWR) of naturalized alien plant species in the hiking-trail of Mount GedePangrango National Park(MGPNP) forest area correlated with leaf traits(specific leaf area(SLA) and leaf thickness) and environmental factors(elevation, slope, and normalized difference vegetation index(NDVI)). We showed that leaf thickness and habitat elevation explained the AWR variations of naturalized alien species. We did not detect any important effect of leaf traits and environmental factors on the number of individuals per exotic species per plot. The influence of leaf thickness and habitat elevation indicates the important role of both biotic and abiotic factors on exotic species to develop a high species richness and become an invasive species in the tropical mountain forest ecosystem.     

南海海域三疣梭子蟹的幼体培育

幼体期Z1和Z2投喂扁藻和轮虫,Z2后期开始以轮虫和卤虫无节幼体混合投喂,Z3后用卤虫无节幼体投喂,大眼幼体用不同饵料培育,进行南海海域三疣梭子蟹幼体培育,结果表明:溞状幼体平均成活率为15.3%;Z2变态为Z3、Z4变态为M为溞状幼体培育的敏感期,幼体的阶段成活率较低;以卤虫无节幼体、冰冻卤虫成虫、活卤虫成虫为饵料,均能使培育大眼幼体至幼蟹,其中活卤虫成虫投喂、加防残网片培育效果最好,成活率达80%。     

Toxicity of five phenolic compounds to brine shrimp Artemia sinica (Crustacea: Artemiidae)

The acute toxicity of five phenolic compounds each to 15 d old Artemia sinica was determined in this study. The brine shrimp A. sinica was hatched from the encysted dry eggs(Bohai Bay Brand) produced by Dongying Ocean Artemia Co., Ltd., China at 27℃± 1℃ in pre-filtered(through pores of 0.45 μm in diameter) and autoclaved seawater(salinity 31, pH 7.5–8.0) in a cilindroconical glass beaker(2000 mL in volume) under continuous illumination(provided by a side set 20 W fluorescent lamp) with slight aeration. Ten Artemia individuals from the same batch of the hatched were cultured in 10 mL toxicant solution prepared with seawater(salinity 31, pH 7.5–8.0) at room temperature(about 20℃) to determine 24 h, 48 h and 72 h medium lethal concentration(LC50) of 5 phenolic compounds each. It was found that the toxicity of n-heptylphenol was the highest followed by nonylphenol, t-butylphenol, 2,4-dichlorophenol and bisphenol A in order. The LC50 values of the 5 compounds were calculated with regression analysis. The real concentration(in μg- L1) of 5 phenolic compounds each in toxicant solutions was measured with GC/MS analysis. Significant loss of phenolic compounds caused by either adsorption or desorption was not found. The significant difference of LC50 values was found among the five compounds 3 exposure times each. The range between the highest no-observed-effect concentration(NOEC) and 100% death causing concentration of five phenolic compounds each was determined. The toxicity in term of 24 h LC50 value of n-HP was 9.10 times higher than that of BPA, 1.71 times higher than t-BP, 1.53 times higher than 2,4-DCP and 1.36 times higher than NP, respectively.     

How can saline and hypersaline lakes contribute to aquaculture development? A review

A considerable part of the world's population is currently experiencing a severe scarcity of freshwater and nutrition. Inland aquaculture has the fastest growth in fresh waters, and this contributes to the eutrophication of freshwater bodies. The increase in freshwater aquaculture impacts on the increasing demand for fresh water. A way to overcome this is to develop aquaculture in saline lakes. This article discusses how saline and hypersaline lakes may contribute to overcome this problem and gives a list of fish and shrimp species that can be cultivated in saline lakes. Successful development of aquaculture depends on a healthy cultured stock of commercial fish and shrimps. A sustainable healthy stock of fish and shrimps can be only maintained using live food for the cultured fish larvae, fry and fingerlings. As well as Artemia spp. there are many other crustacean species with the potential for growing in hypersaline waters. At least 26 copepod species around the world can live at a salinity above 100 g/L with 12 species at a salinity higher than 200 g/L, and these all have excellent nutritional value. There is a high potential to use eukaryotic organisms of diff erent taxa in saline/hypersaline aquaculture for food, agri-aquaculture, diff erent industries and as food supplements.     

Status and historical changes in the fish community in Erhai Lake

Erhai Lake is the second largest freshwater lake on the Yunnan Plateau, Southwest China. In recent decades, a number of exotic fish species have been introduced into the lake and the fish community has changed considerably. We evaluated the status of the fish community based on surveys with multimesh gillnet, trap net, and benthic fyke-net between May 2009 and April 2012. In addition, we evaluated the change in the community using historical data (1952–2010) describing the fish community and fishery harvest. The current fish community is dominated by small-sized fishes, including Pseudorasbora parva, Rhinogobius giurinus, Micropercops swinhonis, Hemiculter leucisculus, and Rhinogobius cliffordpopei. These accounted for 87.7% of the 22 546 total specimens collected. Omnivorous and carnivorous species dominated the community. A canonical correspondence analysis (CCA) plot revealed that the distribution of fishes in the lake is influenced by aquatic plants, water temperature, pH, and season. The abundance of indigenous species has declined sharply, and a majority of endemic species have been extirpated from the lake (a decrease from seven to two species). In contrast, the number of exotic species has increased since the 1960s to a total of 22 at present. The fishery harvest decreased initially following the 1960s, but has since increased due to the introduction of non-native fish and stocking of native fish. The fishery harvest was significantly correlated with total nitrogen, not total phosphorus, during the past 20 years. Based on our results, we discuss recommendations for the restoration and conservation of the fish resources in Erhai Lake.     

THE INDUCTION AND EXTRACTION OF METALLOTHIONEINS IN ARTEMIA

A small amount of heavy metal binding protein,identified by BioRad Protein Assay,has been iso-lated from the adult brine shrimp,Artemia franciscanus.This protein has an apparent molecular weight be-tween 6000 to 9000 dalton.a UV absorption peak at 260 instead of 280 nm like most proteins;andhas high affinity towards binding with radioactive labeled ~(109)Cd.These characteristics are similarto that of metallothioneins reported for many vertebrate and invertebrate,marine and terrestrialanimals.After the brine shrimp is exposed to a small amount of Cd~(2+)for 24 h,a large amount ofmetallothionein can be isolated,showing the inducibility of this detoxifying protein in the adult Artemiain a short period of time.     

EFFECT OF ARTIFICIAL REGULATIONS OF ARTEMIA n-3 HUFA CONTENT ON GROWTH AND SURVIVAL OF BLACK SEABREAM (SPARUS MACROCEPHLUS) LARVAE

ImODUCTIONStudiesonthenutritionmpuirementSoffishlarvaehavernadesomeprognaabroad,butwearenotawareofreportSonsuchstudiesinChinanow.ltwasdeter-Annedanhythatn-3HU-FA,espedally22f6(n-3)roHA)and2O5(n-3)(EPA)ises-sentialformarinefishlarvaegroWth.ThehighcontentofDHAinmarinefisheggSandthel0wcontent0fDHAinArtrmiashowthatArthetaasliwfndcandsaoothen-3HUFAreq~dmendfishlacae.bosdenistSdriitaln-3HUFAenriedArbotabyboingthemn-3HUFAcontainingbogtanulataldietS,etC.Watanabe,T.(l983)enri~livefe…     

远海梭子蟹幼体饵料初步研究

分别选用轮虫、轮虫 卤虫、轮虫 卤虫 扁藻、卤虫、扁藻为饵料 ,将刚孵化的远海梭子蟹氵蚤状幼体培育至幼蟹第二期。结果发现 :轮虫 卤虫 扁藻的效果最好 ,幼蟹第二期的成活率为 15% ,轮虫 卤虫的成活率为 11% ,卤虫的成活率为 10 %。而轮虫组仅能培育至 氵蚤状幼体第三期 ,没有氵蚤状幼体第四期幼体出现 ;扁藻最差 ,仅能培育至氵蚤状幼体第一期 ,没有氵蚤状幼体第二期个体出现。同时发现 ,轮虫 卤虫组与卤虫组培育时间均比轮虫卤虫 扁藻组长 ;各期幼体的阶段成活率比较而言 ,氵蚤状幼体第一期最高 ,而氵蚤状幼体幼体第五期最低     

Anthropogenic Pressure on Structure and Composition of a Shola Forest in Kerala, India

Introduction Tropical region is endowed with a vast tract of diverse vegetation (Gentry 1992). Among different types of forests, the montane forests, due to their unique features including rich biodiversity andprominent ecological services, have always attracted biologists, bio-geographers, ecologists and forest managers (Doumenge et al. 1995). In India, montane forests covering 7.9 % of total forest area occur in the Himalayan, northeastern and southern parts of the country (Lal 1989). In s…