Groundwater depth affects the daily course of gas exchange parameters of Populus euphratica in arid areas

In this case study, the diurnal courses of gas exchange in Populus euphratica at different groundwater depths in the lower reaches of Tarim River were investigated to understand the effects of soil hydrology on photosynthesis and water use efficiency (WUE) of vegetation in arid desert area. It was found that the photosynthetic rate (P _N) was not sensitive to the change of groundwater depth (GD) within the range of 4.2?C6.8?m. Compared to stomatal conductance (g _s) and transpiration rate (E) of P. euphratica grown at GD 4.2, 5.6 and 5.8?m, g _s and E at GD 6.8?m both markedly declined in June, suggesting that P. euphratica at deeper GD can avoid overall water loss by stomatal adjustment. The intrinsic water use efficiency of P. euphratica first decreases with the increasing GD, but when GD increased to 6.8?m, intrinsic WUE increased by 1.2?C2.2 fold, compared with the WUE of P. euphratica at GD 4.2?C5.8?m, indicating that intrinsic WUE of P. euphratica will increase when the plant suffers from moderate drought stress.     

The threshold of soil moisture and salinity influencing the growth of Populus euphratica and Tamarix ramosissima in the extremely arid region

The drought and salt tolerance of two pioneer species, Populus euphratica and Tamarix ramosissima, were studied by monitoring the stem and leaf water potentials under various soil moisture and salinity at depths of 0–180 cm. The plants are naturally distributed in arid environment in the lower reaches of the Tarim River in Xinjiang, China. The results showed that P. euphratica can withhold water by prolonging dewatering and adapt to the dry desert weather by reducing water consumption. The lowest soil moisture that would unfavorably influence its growth was found to be 7% soil moisture. T. ramosissima was found to have low water potential and high transpiration efficiency. It is capable of absorbing water from the soil by keeping a low water potential, so there is no critical limit of soil moisture for T. ramosissima. In terms of salt resistance, P. euphratica was found to secrete salt from its body by discharging salty water through portals in its trunks and leaves. A soil salinity of 20% was the minimum concentration at which the salt secretion mechanism of P. euphratica was activated. T. ramosissima secreted salt by storing the accumulated salt in the vacuoles of its salt secretion glands for separation. Thus, it has no minimum soil salinity limit. T. ramosissima was found to have better resistance to drought and salt stress than P. euphratica.     

Research on the water use efficiency and foliar nutrient status of <Emphasis Type="Italic">Populus euphratica</Emphasis> and <Emphasis Type="Italic">Tamarix ramosissima</Emphasis> in the extreme arid region of China

Seasonal variations in foliar stable carbon composition (δ¹³C) and nitrogen (N), phosphorus (P) and potassium (K) concentrations of Populus euphratica Olivier and Tamarix ramosissima Ledeb, as well as correlations between foliar δ¹³C values and N, P and K concentrations were studied in the Ejina oasis in the lower reaches of the Heihe River, northwestern China. Foliar δ¹³C values, and P and K concentrations significantly differed between species. Foliar δ¹³C signatures in T. ramosissima were significantly more enriched than those in P. euphratica, but P and K concentrations of P. euphratica were higher. The seasonal variations and patterns of these were similar. The total foliar δ¹³C values and N, P and K concentrations in both P. euphratica and T. ramosissima showed a descending trend. There were significantly positive correlations between foliar δ¹³C values and N and P concentration of both P. euphratica and T. ramosissima. For the relationship between foliar δ¹³C and K concentration, there appeared oppositely positive and negative correlations in P. euphratica and T. ramosissima, respectively, but they were not significant. The simple positive correlations of δ¹³C–P% and δ¹³C–N% suggested that N and P in P. euphratica and T. ramosissima behave in a similar manner. This study also showed that there were nutrient deficiencies; N and P nutriments were the co-limiting factors in P. euphratica, while it was P nutriment in T. ramosissima.     

How water use of <Emphasis Type="Italic">Salix</Emphasis><Emphasis Type="Italic">psammophila</Emphasis> bush depends on groundwater depth in a semi-desert area

To investigate the groundwater levels changes effects on the transpiration of Salix psammophila (S. psammophila) bush, systemic measurements of meteorological conditions, sap flow of S. psammophila, soil water contents and groundwater levels were conducted in the Hailiutu River catchment, NW of China. Based on the collected field data, Hydrus-1D software package was used to calibrate water movement for root uptake in the saturated–unsaturated zone. The soil hydraulic parameters and root uptake function parameters were calibrated. The simulated results of soil water contents and sap flow fitted well with the observed ones. Based on the calibrated hydraulic parameters, different groundwater levels were imposed at the low boundary to simulate the groundwater levels changes effects on the transpiration. The relationship between ratio of actual transpiration and potential (T/T _p) and groundwater water table depth was established. The results shows that the ratio of actual transpiration and potential transpiration decreases with groundwater table depth increase as inverse ‘S’ shape. And the turn point is corresponding to the extinct depth, i.e., no groundwater contribution to S. psammophila transpiration. To further verify this phenomenon is universal, the soil hydraulic parameters were replaced with the other five groups in the calibrated forward model. The results confirmed the similar changes of T _a/T _p with the groundwater levels changed, i.e. inverse ‘S’ shape. However, the extinction depth is different corresponding to different soil hydraulic parameters. So, the research results indicated the relationship between transpiration and groundwater levels is non-linear function.     

Photosynthesis and water use efficiency of Populus euphratica in response to changing groundwater depth and CO2 concentration

Changes of stomatal conductance (g _s), net photosynthetic rates (P _N) and water use efficiency (WUE) were investigated in Populus euphratica grown on sites with different groundwater depths (GDs) under two CO₂ concentrations in the lower reaches of Tarim River, Xinjiang, northwestern China. P _N in P. euphratica only slightly decreased when the groundwater depth increased from 4.12 to 7.74 m below the ground surface. P _N values significantly increased in response to an elevated CO₂ concentration at all GDs except at GD of 4.12 m for its good availability of groundwater. WUE values decreased with an initial increase in the groundwater depth, but increased when the groundwater depth reached 7.74 m especially under the elevated CO₂ concentration. The g _s values measured at 4.12 and 4.74 m both decreased indistinctively by only 3% due to CO₂ enrichment; however, when GDs increased to 5.54 and 7.74 m, the g _s values decreased significantly by about 10%. It shows that the response of g _s in P. euphratica to elevated CO₂ is weaker under lower groundwater depth (mild drought stress) but stronger under deeper groundwater depth (moderate drought stress). Results from this study suggest that groundwater depth could determine the response of photosynthesis to future CO₂ enrichment in P. euphratica in arid desert areas.     

Toxicity of methyl parathion on growth and reproduction of three ecologically different tropical earthworms

This work illustrates the toxicological impact of pesticide methyl parathion (MP) (dust—2 % active ingredient, a.i.) on growth and reproduction performance in tropical earthworms: Metaphire posthuma (endogeic), Lampito mauritii (anecic) and Allolobophora parva (epigeic). A total of three concentrations (a.i. g kg^?1 dry test soil), 1.00 (T ₁), 1.125 (T ₂) and 2.25 (T ₃) of MP, were applied in test substrate to examine the impact on mortality, individual live weight changes and reproduction patterns in test species over 60 days under laboratory conditions. MP caused significant mortality in all tested species, and median lethal dose (LD₅₀) for L. mauritii, M. posthuma and A. parva was 24.85, 23.64 and 22.67 mg a.i., respectively. The individual live weight loss was 27.0–37.0 % in L. mauritii, 36.0–57.1 % in M. posthuma and 1.2–11.0 % in A. parva in different test concentrations. The pesticide-exposed worms produced less cocoons than control, but in L. mauritii, an unusual reproduction (hormesis) was recorded. Results suggested the species-specific toxicity of MP against tropical earthworms.     

Physiological acclimation strategies of riparian plants to environment change in the delta of the Tarim River,China

The occurrence and development of riparian forests, which were mainly dominated by mesophytes species related closely with surface water. Since there was no water discharged to the lower reaches of Tarim River in the past three decade years, the riparian forests degrade severely. The groundwater table, the saline content of the groundwater, as well as the content of free proline, soluble sugars, plant endogenous hormones (abscisic acid (ABA), and cytokinins (CTK)) of the leaves and relative rates of sap flow of the Populus euphratica Oliv. (arbor species), Tamarix ramosissima Ldb. (bush species), and Apocynum venetum L. (herb species) were monitored and analyzed at the lower reaches of the Tarim River in the study area where five positions on a transect were fixed at 100 m intervals along a sampling direction from riverbank to the sand dunes before and after water release. The physiological responses and acclimation strategies of three species to variations in water and salinity stress were discussed. It was found that A. venetum population recovered to groundwater table ranging from −1.73 to −3.56 m, and when exposed to saline content of the groundwater ranging from 36.59 to 93.48 m mol/L; P. euphratica appeared to be more sensitive to the elevation of groundwater table than the A. venetum and T. ramosissima at groundwater table ranging from −5.08 to −5.80 m, and when exposed to saline content of the groundwater ranging from 42.17 to 49.55 m mol/L. T. ramosissima tended to be the best candidate species for reclamation in this hyper-arid area because it responded to groundwater table ranging from −1.73 to −7.05 m, and when exposed to saline content of the groundwater ranging from 36.59 to 93.48 m mol/L. These results explained the distribution patterns of desert vegetation in the lower reaches of the Tarim River. Understanding the relationships among ecological factors variables, physiological response and acclimation strategies of plant individuals could provide guidance to sustainable management, reclamation and development of this and similar regions.     

Germination enhancement and inhibition ofDistichlis spicata andScirpus robustus seeds from Viriginia

Germination of mature, viable seeds ofDistichlis spicata (L.) Greene. andScirpus robustus Pursh. from two Virginia salt marshes was not significantly inhibited by aqueous washings from the rhizospheres of sand-culturedPhragmites australis (Cav.) Trin. ex. Stend.,Juncus roemerianus Scheele, orTypha angustifolia L. Germination ofS. robustus seed was inhibited by increased osmotic pressure whileD. spicata germination increased (2.5 fold) significantly when treated with leachate fromT. angustifolia rhizosphere.     

Holocene annual mean temperature changes in Estonia and their relationship to solar insolation and atmospheric circulation patterns

We reconstructed annual mean temperature (T_ann) trends from three radiocarbon-dated Holocene pollen stratigraphies from lake sediments in Estonia, northern Europe. The reconstructions were carried out using a North-European pollen-climate calibration model based on weighted averaging partial least-squares regression. The cross-validated prediction error of the model is 0.89°C and the coefficient of determination between observed modern T_ann values and those predicted by the model is 0.88. In the reconstruction, the Holocene thermal maximum (HTM) is distinguishable at 8000-4500 cal yr B.P., with the expansion of thermophilous tree species and T_ann on average 2.5°C higher than at present. The pollen-stratigraphical data reflect progressively warmer and drier summers during the HTM. Analogously with the modern decadal-scale climatic variability in North Europe, we interpret this as an indication of increasing climatic continentality due to the intensification of anticyclonic circulation and meridional air flow. Post-HTM cooling started abruptly at around 4500 cal yr B.P. All three reconstructions show a transient (ca. 300 years) cooling of 1.5-2.0°C at 8600-8000 cal yr B.P. We tentatively correlate this cold event with the North-Atlantic “8.2 ka event” at 8400-8000 cal yr B.P. Provided that the 8.2 ka event was caused by freshening of the North-Atlantic surface water, our data provide evidence of the climatic and vegetational responsiveness of the boundary of the temperate and boreal zones to the weakening of the North-Atlantic thermohaline circulation and the zonal energy transport over Europe. No other cold events of comparable magnitude are indicated during the last 8000 years.     

黑河下游天然胡杨树干液流特征的试验研究

应用热扩散原理,采用ICT2000TE 研究了黑河下游25 a生天然胡杨(Populus euphratica)的树干液流特点及其与环境因子的关系.结果表明:1) 在整个生长季的晴天,胡杨树干液流的日变化呈现明显的单峰宽峰曲线.液流速率在6、7、8、9和10月分别为13.39、12.07、12.69和5.1 L·d-1;2) 胡杨在夜间有微弱上升液流,可能存在根压;3) 在生长季树干液流速率与环境因子逐步回归分析结果表明,树干液流受环境因子的综合影响,影响大小依次是空气温度、土壤含水量、相对湿度、总辐射、土壤温度和风速.     

The pollen complex from postglacial sediments of the Laptev Sea as a bioindicator

The first results of comparison of palynological analysis (pollen of terrestrial plants), SEM analysis of pollen morphology, and radiocarbon age dating (AMS14C) of sediments of the eastern shelf of the Laptev Sea show that the diverse taxonomic composition of pollen spectra provides an integrated idea of the vegetation and climate of the region over 11.2 calendar kiloyears. It is found that phases of the tree and shrub vegetation development (maxima of pollen of Betula sect. Nanae and Pinus s/g Haploxylon) correspond to the warm epochs in the Holocene. It is obvious that birch phytocoenoses first settled in the southern tundra subzone with increasing temperature, and then coniferous communities of forest tundra. An occurrence of pollen of shrubby birches (Nanae) suggests compliance of permafrost landscapes with cold climate conditions, i.e., with an annual average temperature of ?2°C and amount of precipitation of less than 500 mm. Owing to a progressive increase in summer temperatures, dwarf cedar and pine communities advanced toward the seashore. The SEM analysis results show that a significant proportion of regional coniferous pollen belongs to representatives of Pinus pumila (Pall.) and P. sylvestris L. In addition, the SEM study of the exine of Pinus sylvestris L. and P. pumila (Pall.) Regel pollen grains confirmed polymorphism in coniferous pollen. According to the inverse relationship between climate and vegetation, frequent climate fluctuations that are typical of progressive and differential postglacial transgression were revealed. An increase in arboreal pollen transfer onto the shelf later than 9.1 cal. ka coincides with the time of forest boundary migration to the north due to the warming of the Earth’s climate. At that time, the tundra vegetation was replaced by forest-tundra vegetation, the maximum stage of sea transgression began, and there appeared a trend of increasing temperature and moisture.     

The genus Perissocytheridea Stephenson, 1938 (Crustacea: Ostracoda) and evidence of brackish water facies along the Oligo-Miocene,Pirabas Formation,eastern Amazonia,Brazil

Perissocytheridea Stephenson is characteristic of brackish water facies. In 57 samples from the Upper Oligocene to Lower Miocene, from five localities, Pirabas Formation, Pará State, Brazil eleven species have been identified. Among these species, four are new reports: Perissocytheridea punctoreticulata n. sp., Perissocytheridea largulateralis n. sp., Perissocytheridea colini n. sp. and Perissocytheridea pirabensis n. sp.; five species in open nomenclature: Perissocytheridea sp. 1, P. sp. 2, P. sp. 3, P. sp. 4, and P. sp. 5 and two species left in “aff.” abbreviation: Perissocytheridea aff. Perissocytheridea pumila and Perissocytheridea aff. Perissocytheridea brachyforma subsp. excavata. The distributional pattern of the Perissocytheridea combined with the occurrence of foraminifera Elphidium and Ammonia in the studied sections supports the presence of the brackish water facies to the respective layers. Their quantitative variation through the studied sections indicate more than one phase of salinity reduction (about >5 and <30 ppm) and/or the decrease of the sea level, forming lagoon along the Pirabas Formation. This genus has a wide paleobiogeographical occurrence and stratigraphic distribution ranging from the Cretaceous to Recent, and already been recorded in the northern of South America, especially in the Neogene of Solimões Basin, but this is the first report of a neotropical genus to the Oligo-Miocene deposits of Pirabas Formation, northern Brazilian Coast, Pará State.     

热脉冲技术在确定胡杨幼树干液流中的应用

探讨了热脉冲技术在确定胡杨(Populus euphratica)幼树干液流中的应用.结果表明:在树木正常生长状态下,胡杨在4月下旬至5月中旬开花期树干单位面积液流通量为0.13～0.15L·cm^-2·d^-1;树干液流速度的动态变化呈多峰状曲线,6月份枝叶速生期树干单位面积液流通量为0.294L·cm^-2·d^-1;树干径向断面形成层以下不同深度的树液流速具有相同的日变化趋势;夜间,胡杨存在明显的树干液流上升现象,补充白天植物蒸腾丢失的大量水分,恢复植物体内的水分平衡;树干液流速度在不同的方位有所差异.并对胡杨单位面积液流通量与杨树、辽东栎、大叶白蜡、棘皮桦、五角槐、小美旱杨、油松进行了对比研究.     

Effects of flooding regimes on the decomposition and nutrient dynamics of Calamagrostis angustifolia litter in the Sanjiang Plain of China

From May 2005 to September 2006, the potential effects of marsh flooding regimes on the decomposition and nutrient (N, P) dynamics of Calamagrostis angustifolia litter were studied in the typical waterlogged depression in the Sanjiang Plain, Northeast China. The decomposition of C. angustifolia litter was related to four sites with different hydrologic regimes [F1 (perennial flooding, average water depth of 480?days was 40.14?±?8.93?cm), F2 (perennial flooding 33.27?±?6.67?cm), F3 (perennial flooding 23.23?±?5.65?cm) and F4 (seasonal flooding 1.02?±?1.09?cm)]. Results showed that flooding regimes had important effects on the litter decomposition, the decomposition rates differed among the four sites, in the order of F3 (0.001820d^?1)?>?F1 (0.001210d^?1)?>?F2 (0.001040d^?1)?>?F4 (0.000917d^?1), and the values in the perennial flooding regimes were much higher. Flooding regimes also had significant effects on the N and P dynamics of litter in decomposition process. If the perennial flooding regimes were formed in C. angustifolia wetland due to the changes of precipitation in the future, the litter mass loss would increase 23.28?C48.88%, the decomposition rate would increase 13.41?C98.47%, and the t _0.95 would decrease 1.07 yr?C4.50 yr. In the perennial flooding regimes, the net N accumulated in some periods, while the net P released at all times. This study also indicated that the changes of N and P content in the litter of the four flooding regimes were probably related to the C/N or C/P ratios in the litter and the N or P availability in the decomposition environment. If the nutrient status of the decomposition environment did not change greatly, the decomposition rates depended on the substrate quality indices of the litter. Conversely, if the nutrient status changed greatly, the decomposition rates might depend on the supply status of nutrient in the decomposition environment.     

Calibration of the carbonate ‘clumped isotope’ paleothermometer for otoliths

Paleothermometry is an essential tool for understanding past changes in climate. The ‘carbonate clumped isotope thermometer’ is a temperature proxy related to ordering of ¹³C and ¹⁸O in the carbonate lattice (based on measurements of ¹³C¹⁸O¹⁶O in CO₂ produced by acid digestion of carbonate). This thermometer has been previously calibrated for inorganic calcite and aragonitic corals [Ghosh P., Adkins J., Affek H., Balta B., Guo W. F., Schauble E. A., Schrag D., and Eiler J. M. (2006) C-13-O-18 bonds in carbonate minerals: a new kind of paleothermometer. Geochim. Cosmochim. Acta70 (6), 1439-1456]. Here we determine the relationship between growth temperatures of aragonitic fish otoliths and abundances of ¹³C¹⁸O¹⁶O produced by acid digestion of those otoliths. Our calibration is based on analyses of otoliths from six species from four genera of modern fish sampled from a latitudinal transect of the Atlantic Ocean between 54° S and 65° N, plus one species from the tropical western Pacific. The temperatures at which fish otoliths precipitated were estimated by the mean temperature in the waters in which they lived, averaged over their estimated lifetimes. Estimated growth temperatures of our samples vary between 2 and 25 °C. Our results show that the abundance of ¹³C¹⁸O¹⁶O in CO₂ produced by acid digestion of fish otolith aragonite is a function of growth temperature, following the relationship: , where Δ₄₇ is the enrichment, in per mil, of ¹³C¹⁸O¹⁶O in CO₂ relative to the amount expected for a stochastic (random) distribution of isotopes among all CO₂ isotopologues, and T is the temperature in Kelvin. This relationship closely approaches that previously documented for inorganic calcite and aragonitic coral (Ghosh et al., 2006).     

Specific affinity for phosphate uptake and specific alkaline phosphatase activity as diagnostic tools for detecting phosphorus-limited phytoplankton and bacteria

We analyzed responses of soluble reactive phosphorus (SRP), bioavailable phosphate (PO₄), particulate phosphorus, turnover time of orthophosphate (T_t), and alkaline phosphatase activity (APA) to varying degrees of nutrient stress. The nutrient stress was evoked by different treatments in concentration and combination of inorganic nitrogen (N) and phosphorus (P), and labile organic carbon (glucose) to mesocosms in experiments carried out in eutrophic southern (Odense Fjord, Denmark) and northern (Tvärminne Archipelago, Finland) coastal zones of the Baltic Sea. Despite seasonal and geographical differences, similar responses were observed in both experiments. Low SRP (<100 nmol l^?1), shortT _t (<10 h), and increased levels of APA were observed in both N+P balanced and P deficient treatments, while the opposite trend was observed in P replete treatments. The shortestT _t and the highest APA were found when glucose was combined with N treatment. Bioavailable PO₄ was estimated usingT _t and P uptake rates as derived from stoichiometric conversion of carbon based primary and bacterial production. With shorterT _t, the PO₄ pool declined to <1 nmol-P l^?1, whereas the SRP background pool (difference between SRP and PO₄) remained relatively constant (c. 50 nmol l^?1). APA was inversely related to PO₄ but not to SRP. Responses of specific APA and specific affinity for PO₄ uptake, which are APA and PO₄ uptake rates (inverse ofT _t), respectively, normalized to the summed P biomass of phytoplankton and bacteria, responded consistently to the pattern and magnitude of nutrient limitation evoked in our experiments. Our results, together with a literature survey, suggest that both parameters can be useful in examining PO₄ availability for the natural phytoplankton and bacteria community in P starved aquatic systems.     

The influence of 10 years of water conveyances on groundwater and juvenile Populus euphratica of the lower Tarim River

The changes in the depth of groundwater were studied from the period before water conveyance through 2010, and the TDS of groundwater was analyzed in the lower reaches of the Tarim River during the period 2001–2010. Besides, the numbers of juvenile P. euphratica were investigated in the lower reaches of the Tarim River during the period 2001–2010. The findings indicate that: (1) the hydrological environment of the lower reaches has slowly deteriorated again; and (2) few juvenile P. euphratica were observed along the channel. These results show that the positive influence of the ecological water conveyance project on the ecosystem of the lower Tarim River should be beneficial in the long term; however, the long-term stability of the ecosystem cannot be achieved by the current water diversion scheme.     

Restoration of an impounded salt marsh in New England

The restoration of a 20 ha tidal marsh, impounded for 32, yr, in Stonington, Connecticut was studied to document vegetation change 10 yr after the reintroduction of tidal flushing. These data were then compared to a 1976 survey of the same marsh when it was in its freshest state and dominanted byTypha angustifolia. Currently,T. angustifolia remains vigorous only along the upland borders and in the upper reaches of the valley marsh. Live coverage ofT. angustifolia has declined from 74% to 16% and surviving stands are mostly stunted and depauperate. Other brackish species have also been adversely effected, except forPhragmites australis which has increased. In contrast, the salt marsh speciesSpartina alterniflora has dramatically expanded, from <1% to 45% cover over the last decade. Locally, high marsh species have also become established, covering another 20% of the marsh.     

Chemical reactions and porosity changes during sedimentary diagenesis

It is of interest to simulate the porosity changes associated with fluid flow and water–rock reactions in sedimentary systems. The chemical species taken into account are: {H₂O, H⁺, Ca⁺⁺, HCO₃⁻, Mg⁺⁺, Al(OH)₄⁻, H₄SiO₄, K⁺, Na⁺, Cl⁻}. The solution is assumed to be locally at equilibrium with different mineral assemblages including at least calcite, dolomite and one Mg–Al-silicate. These assemblages buffer the partial pressure of CO₂ (Pco₂ ) which increases with temperature (T) as is commonly observed in natural systems. The nature of the Mg–Al-silicate and the presence or absence of other at-equilibrium minerals (i.e. the feldspars) do not modify the Pco₂–T relationship. Considering complete transfer between the solution and the solid phases, computed relative porosity increases with increasing temperature for all the systems that were investigated. However, the direction and the amount of the clay transfer depend on the presence of feldspars. Furthermore, it is shown that computing mass transfer using a prescribed Pco₂, as is commonly done, leads to very different values of mineral transfers. Hence, it must be concluded that prescribing a Pco₂ value, even if the proper Pco₂–T relationship is used, leads to erroneous results.     

The effects of wastewater reuse on potato growth properties under greenhouse lysimeteric condition

In this study, raw and treated wastewaters were reused for potato cultivation in order to verify the effect of wastewater on crop yield, crop’s heavy metals’ concentration as well as some major traits of potato. To this regard, a completely randomized test was designed with five water treatments and three replications. The watering were as follows: raw wastewater (T₁), treated wastewater (T₂), a combination of 50 % raw wastewater and 50 % fresh water (T₃), a combination of 50 % treated wastewater and 50 % fresh water (T₄), and fresh water (T₅). The experiments were run during October 2009–June 2010 in the greenhouse of Bu-Ali Sina University. The results show that the effects of treatments were significant on the length and number of stems per plant (p < 0.05). The number of nodes and weight of tubers, crop yield and heavy metal (cadmium, nickel and lead) concentration in shoots and tubers were also significant (p < 0.01). The results indicated that the highest length of stem (55.44 cm) was obtained in T₂ which had no significant differences from that of T₁. The maximum and minimum tuber weights and crop yield were obtained in T₁ and T₅, respectively. Based on crop yield rate, the watering ranked as follows: T₁ > T₃ > T₂ > T₄ > T₅. The maximum and minimum heavy metal values were observed in T₁ and T₅, respectively. Based on the cadmium, nickel and lead accumulations in shoots and tubers (except cadmium in shoots), the watering treatments ranked as: T₁ > T₃ > T₂ > T₄ > T₅.