盐度对口虾蛄仔虾摄食、存活和掘穴的影响

为研究口虾蛄(Oratosquilla oratoria)仔虾在人工养殖条件下的盐度适应性,作者分别实验分析了盐度突变和渐变对其累积存活率、摄食率和掘穴行为的影响。实验结果表明,仔虾盐度耐受能力较强,突变组的仔虾存活盐度为15~45,渐变组的仔虾存活盐度为5~54;仔虾的适宜盐度为21~33,24 h存活率在80%以上;盐度对口虾蛄的摄食影响较大,突变盐度为21~33时,仔虾摄食较为活跃,低于18或高于42基本不摄食;盐度渐变组摄食活跃范围为19~38,低于9或高于50基本不摄食;盐度突变对口虾蛄仔虾掘穴的影响显著,盐度突变至21和33时,掘穴时间极显著延长。本实验结果可以为口虾蛄的人工养殖和增殖放流提供参考和依据。     

Feeding intensity and molecular prey identification of the mantis shrimp,Oratosquilla oratoria (De Haan, 1844), in the Tianjin coastal zone of Bohai Bay

Bohai Bay, a representative semi-enclosed bay of China, has been severely impacted in recent decades by rapid human development. Although the mantis shrimp Oratosquilla oratoria plays an important role in the Bohai Bay ecosystem and fishery, its feeding ecology and the impact of habitat changes on its feeding habits are poorly known. In this study, we sought to identify its feeding strategy through the separation of stomach contents, and to describe its trophic ecology during maturation in the Tianjin coastal zone of Bohai Bay by the DNA barcoding method using a fragment (about 690 bp) of the mitochondrial cytochrome oxidase I (CO I) gene. A total of 594 specimens were collected from March to July 2018, and 347 (58.42%) stomachs were found to have food remains. More than half of the O. oratoria individuals had poor feeding activity, and the degree of feeding of females was higher than that of males, but there was no significant difference in the visual fullness index and the fullness weight index (FWI) between sexes for each month. In addition, the feeding activity of O. oratoria was consistent across the months, suggesting in artificial breeding programs it is not worried too much about the accessional diet supplied to the female bloodstocks except maintaining their normal food. A total of 167 retrieved prey items yielded 187 readable sequences; 86.63% of these sequences could be assigned to the species level. Consequently, 25 different prey taxa covering all readable sequences were identified, showing O. oratoria is a generalist predator. In terms of percent composition by frequency of occurrences (%N), crustaceans and fishes accounted for the same and highest percentage (35.83%) in the mantis shrimp diet, followed by mollusks (24.59%) and the remaining marine organisms (3.73%) including Polychaeta (2.67%), Sagittoidea (0.53%), and Chromadorea (0.53%). The result shows the benthic fish Oxudercidae (family) appears to be an especially important food supply for O. oratoria during maturation. The degree of cannibalism decreased with an increase in diet species and the frequencies of cannibalism in this study was noticeably higher than that in previous studies, suggesting that cannibalism may be a significant feeding strategy in the mantis shrimp O. oratoria in the Tianjin coastal zone of Bohai Bay, and because of the frequently cannibalistic behavior, the high stocking density of broodstocks should be avoided.     

海州湾及邻近海域口虾蛄栖息地适宜性建模的环境变量优化

Habitat suitability index(HSI) models have been widely used to analyze the relationship between species abundance and environmental factors, and ultimately inform management of marine species. The response of species abundance to each environmental variable is different and habitat requirements may change over life history stages and seasons. Therefore, it is necessary to determine the optimal combination of environmental variables in HSI modelling. In this study, generalized additive models(GAMs) were used to determine which environmental variables to be included in the HSI models. Significant variables were retained and weighted in the HSI model according to their relative contribution(%) to the total deviation explained by the boosted regression tree(BRT). The HSI models were applied to evaluate the habitat suitability of mantis shrimp Oratosquilla oratoria in the Haizhou Bay and adjacent areas in 2011 and 2013–2017. Ontogenetic and seasonal variations in HSI models of mantis shrimp were also examined. Among the four models(non-optimized model, BRT informed HSI model,GAM informed HSI model, and both BRT and GAM informed HSI model), both BRT and GAM informed HSI model showed the best performance. Four environmental variables(bottom temperature, depth, distance offshore and sediment type) were selected in the HSI models for four groups(spring-juvenile, spring-adult, falljuvenile and fall-adult) of mantis shrimp. The distribution of habitat suitability showed similar patterns between juveniles and adults, but obvious seasonal variations were observed. This study suggests that the process of optimizing environmental variables in HSI models improves the performance of HSI models, and this optimization strategy could be extended to other marine organisms to enhance the understanding of the habitat suitability of target species.     

Long-term variation of storm surge-associated waves in the Bohai Sea

When investigating the long-term variation of wave characteristics as associated with storm surges in the Bohai Sea,the Simulating Waves Nearshore(SWAN)model and Advanced CIRCulation(ADCIRC)model were coupled to simulate 32 storm surges between 1985 and 2014.This simulation was validated by reproducing three actual wave processes,showing that the simulated significant wave height(SWH)and mean wave period agreed well with the actual measurements.In addition,the long-term variations in SWH,pattems in SWH extremes along the Bohai Sea coast,the 100-year retum period SWH extreme distribution,and waves conditional probability distribution were calculated and analyzed.We find that the trend of SWH extremes in most of the coastal stations was negative,among which the largest trend was-0.03 m/a in the western part of Liaodong Bay.From the 100-year return period of the SWH distribution calculated in the Gumbel method,we find that the SWH extremes associated with storm surges decreased gradually from the center of the Bohai Sea to the coast.In addition,the joint probability of wave and surge for the entire Bohai Sea in 100-year return period was determined by the Gumbel logistic method.We therefore,assuming a minimum surge of one meter across the entire Bohai Sea,obtained the spatial SWH distribution.The conclusions of this study are significant for offshore and coastal engineering design.     

黄、渤海断面及海洋站的盐度分布特征与变化趋势

采用经验正交函数(EOF)分解、超前滞后相关分析等方法,分析了36°N断面、大连—成山头断面和渤海中部断面盐度的空间分布特征和时间变化特征,讨论了环黄、渤海海洋站之间盐度的关系。结果表明,36°N断面、大连—成山头断面和渤海中部断面盐度的整体趋势都是升高,黄河流量的减小是造成渤海盐度升高的重要原因。黄海千里岩站1960～2000年间的盐度整体趋势也是升高,年变化率为0.01/a,造成盐度升高的主要原因是降水的减少,并且其短期震荡可能和ElNio有关,长期变化则与太平洋年代际震荡(PDO)有关。此外,千里岩站和其他海洋站之间的盐度有较好的正相关关系,只是在时间上存在着1～3个月的超前。对黄、渤海盐度长期变化的系统分析表明,近海盐度可能受到外洋的影响,有助于加深对中国近海环境演变的认识。     

小清河河口邻近海域口虾蛄(Oratosquilla oratoria)COI序列特征及遗传多样性分析

受河流沿岸工农业发展以及人口急剧增加影响,小清河河口及邻近海域受到严重污染。口虾蛄(Oratosquilla oratoria)作为小清河河口及邻近海域重要渔业资源之一,承受着巨大的捕捞和生存压力。为了掌握口虾蛄种群在该区域的种群遗传多样性,本研究于2020年6—10月在小清河河口及邻近海域采集口虾蛄生物样本,进行了基于线粒体COI基因的种群遗传多样性分析。研究结果表明,基于216条长度为655bp的COI基因片段,共定义90个单倍型,63个多态位点,其中包括59个转换位点,1个颠换位点和3个转换与颠换同时存在的位点,核苷酸多样性指数和单倍型多样性分别为0.9700和0.0051。与其他海域相比,小清河河口种群遗传多样性处于相对较高水平;同时遗传结构分析发现黄渤海与东海及南海分布的口虾蛄种群遗传变异较大,但黄渤海海域内遗传变异较小。通过本研究基本掌握了小清河河口及邻近海域口虾蛄种群遗传多样性背景,也可为口虾蛄资源管理以及种质资源库建立等提供科学的基础理论依据。     

渤海沉积物中细菌群落结构和基因丰度的空间分布特征

This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants.     

Long-term changes in summer phytoplankton communities of the open northern Baltic Sea

Changes in the biomass and species composition of phytoplankton may reflect major shifts in environmental conditions. We investigated relationships between the late summer biomass of different phytoplankton taxa and environmental factors, and their long-term (1979–2003) trends in two areas of the Baltic Sea, the northern Baltic proper (NBP) and the Gulf of Finland (GF), with statistical analyses. An increasing trend was found in late summer temperature and chlorophyll a of the surface water layer (0–10 m) in both areas. There was also a significant decrease in summer salinity and an increase in winter dissolved inorganic nitrogen to phosphorus (DIN:DIP) ratio in the NBP, as well as increases in winter DIN concentrations and DIN:SiO₄ ratio in the GF. Simultaneously, the biomass of chrysophytes and chlorophytes increased in both areas. In the NBP, also the biomass of dinophytes increased and that of euglenophytes decreased, whereas in the GF, cyanobacteria increased and cryptophytes decreased. Redundancy analysis (RDA) indicated that summer temperature and winter DIN concentration were the most important factors with respect to changes in the phytoplankton community structure. Thus, the phytoplankton communities seem to reflect both hydrographic changes and the ongoing eutrophication process in the northern Baltic Sea.     

近20年渤海叶绿素a浓度时空变化

浮游植物作为食物链的基础,对海洋生态系统具有重要作用。渤海作为我国最大的内海和重要渔业生物的产卵场、育幼场和索饵场,该区浮游植物研究具有重要意义。叶绿素a浓度是反映浮游植物生物量的重要指标。利用Google Earth Engine平台,对1997–2010年的宽视场海洋观测传感器(SeaWiFS)叶绿素a浓度数据和2002–2018年的水色卫星中分辨率成像光谱仪传感器(MODIS Aqua)叶绿素a浓度数据进行合并,并研究其时空变化特征。研究表明,近20年来,渤海全年叶绿素a浓度增加了14.1%,且增加显著。叶绿素a浓度在所有季节都呈现增加趋势;除11月外,其他各月都呈现稳定或增加趋势。从滦河入河口沿岸至渤海海峡的渤海中部,叶绿素a浓度增加较明显。同时也分析了海洋表面温度、风速和降水量数据。夏季渤海周边区域降水量和风速增加以及秋季海表温度的降低都有助于同季叶绿素a浓度的升高。渤海浮游植物可能受陆源营养物质输入影响较大。     

近百年来渤、黄、东海陆架冲淤作用强度数值研究

数值模拟了渤，黄，东海陆架区潮流动力场作用下9种无黏性单粒径泥沙的冲淤作用强度，计算所得渤，黄，东海陆架近百年来的冲淤分布与实测底质类型的分布多数海区相一致，这表明渤，黄，东海陆架的潮流动力场在该区泥沙输运与淤积中起着主导作用，是该区近百年来冲淤作用强度分布格局的主要控制因素。     

渤黄海营养盐结构及其潜在限制作用的时空分布

根据2006-2007年4个季节的现场调查资料,分析探讨了渤海和黄海营养盐结构分布变化特征及其对浮游植物生长的潜在的限制状况.结果表明,渤黄海水体 Si/N/P 比值均偏离 Redfield 比值,季节变化明显;春夏冬季 N/P和 Si/P比值由近岸向远岸海域递减,高值区主要分布在黄河口、鸭绿江口及苏北近岸,秋季上层水体N/P和Si/P比值的分布趋势有所不同,高值区主要分布在南黄海的中部海域.受陆源输入的影响,近岸特别是河口区 N/P和 Si/P比值均较高,温跃层的生消变化和生物活动调控着黄海中部海域营养盐结构的变化.渤黄海浮游植物生长主要受P的潜在限制,部分季节受N、Si的潜在限制;营养盐限制状况存在着明显的时空变化及不同营养盐的同时或交替限制的现象.     

渤海海区COD分布及对海水富营养化贡献分析

于2002年8月10～18日和2003年8月12～28日对渤海海区进行了海水水质调查。结果表明,调查海域化学耗氧量(COD)平均浓度分别为0.70 mg/L±0.21 mg/L和0.63 mg/L±0.18 mg/L,其中相对高值区(>1 mg/L)主要分布在辽东湾北部,莱州湾南部和中央海区西北部海域,而相对低值区(<0.6 mg/L)主要分布在中央海区东南部。分析表明,调查海域COD浓度不仅远远低于国家一类海水水质标准,而且也大大低于渤海沿岸水。进一步分析表明,COD对于调查海域富营养化的贡献仅为1/4左右,这一结果证明了造成渤海海区富营养化的化学污染物不是COD。     

2010年5月与2013年6月黄、渤海大型底栖动物群落组成和结构的比较研究

基于2010年5月和2013年6月采集的黄、渤海海域的大型底栖动物数据,对该海域大型底栖动物的种类组成和群落结构等进行了研究,对该海域大型底栖动物群落结构的时间和空间差异进行了分析,并利用AMBI和m-AMBI生物指数对各站位的生态质量状况进行了评估。研究结果表明:在2010年5月和2013年6月所采站位的大型底栖动物共有131种,其中软体动物和甲壳动物占据了较大比例的物种数量,分别为51种和42种;其次为鱼类、多毛类和棘皮动物。该海域的优势物种为日本鼓虾 Alpheus japonicus、日本褐虾 Crangon hakodatei、司氏盖蛇尾Stegophiura sladeni和奇异指纹蛤Acila mirabilis,其中司氏盖蛇尾具有最大的丰度和优势度。生物指数(AMBI和m-AMBI)的结果显示黄、渤海区域底栖生态环境仍相对稳定,但与历史资料比较得知,该海域大型底栖动物生存环境整体趋于恶化。     

黄海中部太平洋磷虾的大小组成和丰度分布的季节变化及其与环境因子的关系

The seasonal size structure and spatial abundance distributions of Euphausia pacifica populations were investigated in the central part of southern Yellow Sea from August 2009 to May 2010.The abundance and biomass of E.pacifica were higher in spring and summer,and lower in autumn and winter.The mean abundance and biomass(calculated by carbon)were 74.94 ind./m~3 and 8.23 mg/m~3,respectively.Females with total length(TL)ranging between 10 and 19 mm in summer had a substantial contribution to the population biomass,whereas larvae of TL of 3–7 mm in spring were the main contributor to the population abundance.The sex ratio(female:male)showed a female bias in four seasons.Its value peaked in summer,and then decreased in autumn,spring,and winter successively.Cohort analysis revealed that the length-frequency distribution of E.pacifica could be characterized as one group with large animals(mean TL12 mm)accompanied by one or two subgroups of small individuals(mean TL7 mm).Regarding the spatial distribution,juveniles and adults of E.pacifica tend to concentrate in relatively deep water with low temperature(～11℃)and high salinity(32),whereas its larvae showed more abundance in inshore water with rich chlorophyll a,low salinity(32),and warm temperature(11℃),especially in summer and autumn.Associations changed seasonally between stage-specific abundance and environmental factors.     

Long-term trend analysis of wave characteristics in the Bohai Sea based on interpolated ERA5 wave reanalysis from 1950 to 2020

Reasonably understanding of the long-term wave characteristics is very crucial for the ocean engineering. A feedforward neural network is operated for interpolating ERA5 wave reanalysis in this study, which embodies a detailed record from 1950 onwards. The spatiotemporal variability of wave parameters in the Bohai Sea, especially the significant wave height (SWH), is presented in terms of combined wave, wind wave and swell by employing the 71 years (1950–2020) of interpolated ERA5 reanalysis. Annual mean SWH decreases at ?0.12 cm/a estimated by Theil-Sen estimator and 95th percentile SWH reflecting serve sea states decreases at ?0.20 cm/a. Inter-seasonal analysis shows SWH of wind wave has steeper decreasing trend with higher slopes than that of swell, especially in summer and winter, showing the major decrease may attribute to the weakening of monsoon. The inner Bohai Sea reveals a general decreasing trend while the intersection connecting with the Yellow Sea has the lower significance derived by Mann-Kendall test. Meanwhile, 95th percentile SWH decreases at a higher rate while with a lower significance in comparison with the mean state. The frequencies of mean wave directions in sub-sector are statistically calculated to find the seasonal prevailing directions. Generally, the dominant directions in summer and winter are south and north. A similar variation concerning to SWH, the trend of the mean wave period is provided, which also shows a decrease for decades.     

渤海中部断面环境要素分布及其变化趋势

研究渤海的可持续发展.根据 1979～1999 年渤海中部断面温度、盐度、溶解氧、营养盐的调查资料,分析了它们的分布结构和20年来的变化趋势.结果显示断面各个要素冬季的垂向分布均匀,夏季有2个冷水中心,盐度、溶解氧、营养盐在冷中心位置也出现了类似的高值或者低值中心.各要素的距平图显示盐度有上升趋势,溶解氧含量下降,无机氮逐年增加,磷酸盐和硅酸盐夏季有下降的趋势,但是冬季没有明显的增加或者减少的趋势.     

黄、渤海沉积物中陆源脂类有机质的组成分布与转化特征

本文对黄、渤海表层沉积物中脂类化合物的组成进行了分析研究。结果表明,黄、渤海表层沉积物有机碳的含量为0.03%~1.02%,以黄河口含量最低,黄河口外和双台子河口外,以及北黄海和南黄海中部含量较高。有机质碳稳定同位素δ13C的分布为-21.55‰~-24.28‰,表征其陆海双重来源特征,并且海源特征由河口向海、由近岸向离岸逐渐增强。陆源脂类化合物以河口处略高,河道处略低,由河口向渤海中部和靠近渤海海峡方向迅速降低,表现出显著的河流输入特征;其形态组成由河道的游离态接近100%的绝对优势,迅速转变为渤海中部的以皂化结合态为主（大于50%）,仅在渤海中部和渤海海峡附近存在小于5%的矿物结合态,转化程度较低。北黄海中部和南黄海中部泥质区的有机碳和陆源脂类化合物显著富集,但其对总有机碳的相对贡献较小;其中皂化结合态超过50%,矿物结合态小于10%,说明其具有中等偏高的转化程度。     

渤海、黄海、东海M2潮汐潮流的三维数值模拟

利用建立的一种新的半隐半显三维数值格式,将渤海、黄海、东海作为一个整体,采用球面坐标系下的三维潮波方程组,考虑了引潮力的作用,数值模拟了渤海、黄海、东海的M₂分潮的潮汐与潮流,结果较好地体现了渤海、黄海、东海M₂分潮的特征.通过比较65个验潮站的实测值与计算值,所得计算结果的振幅差平均为6.4cm,相角差为6.1°,计算与实测符合良好.本文给出的问潮图与Fang于1986年给出的实测占数值综合结果基本一致.对选取的47个测流站,比较了各层潮流调和常数Ucosζ、Usinζ、Vcosη、Vsinη的计算值与实测值的偏差,偏差绝对值的平均在2.6～4.9cm/s之间.并比较分析了潮流的垂直结构,所得结果与实测符合较好.首次揭示出回流点的水平位置不随深度变化这一特性.最后给出了M₂分潮的潮能消耗.     

渤海大型底栖动物种类组成与群落结构研究

本文利用2008年8月在渤海23个站位采集的样品对大型底栖动物的丰度、生物量、群落结构及其与环境因子的关系进行了研究。共鉴定出大型底栖动物300种,主要类群包括环节动物多毛类、节肢动物甲壳类、软体动物、棘皮动物等。研究海域大型底栖动物的平均丰度为1 094.7ind/m2,平均生物量为11.78g/m2,其中丰度最高区位于辽东湾,最低区位于渤海湾;生物量最高区位于渤海中部,最低区出现在渤海海峡湾口处。依据丰度和生物量进行了CLUSTER聚类和MDS标序图分析,结果可将研究站位划分为6个站组,均与沉积物底质类型密切相关。所研究海域底质类型包括黏土质粉砂、砂、砂质粉砂、粉砂质砂、砂-粉砂-黏土5种。通过BIOENV分析,发现影响大型底栖动物群落结构和分布的主要环境因子有水深、黏土粉砂含量、脱镁叶绿酸含量、叶绿素含量等。与20世纪90年代历史数据的对比发现,本次研究大型底栖动物的物种数目明显减少,平均丰度也减少,特别是优势物种表现出了小型化趋势,传统的大型种类,如心形海胆、双壳类等,被小个体的多毛类和甲壳类所取代。本研究表明渤海近年来受人类活动影响,出现了不同程度的污染,导致大型底栖动物群落结构发生了变化。     

渤海岸线及水深变化对水动力影响的数值模拟

为探究渤海岸线及水深变化对水动力的影响,基于Delft3D水动力学模型,选用2003年和2015年作为围 填海前后的典型年份,建立了围填海前后岸线及水深条件下的渤海三维水动力模型,并对水动力场进行了模拟。通过对围填海前后潮波和潮余流的分析,得到了岸线及水深变化对渤海水动力场的影响。结果表明:填海后,岸线及水深变化会对渤海主导分潮M₂分潮产生较大影响,秦皇岛附近无潮点向西北方向偏移,渤海海域M₂ 分潮振幅总体减小;潮致余流场受岸线及水深变化影响较大,其中渤海湾曹妃甸港南部形成复杂的涡流,沿岸海域余流增大;滨海新区附近形成多个小范围环流,且天津港到黄骅港北部沿岸海域2015年余流比2003年增加3~5 cm/s;黄骅港南部形成一个逆时针环流,并且该处余流减小2~5 cm/s。辽东湾辽河口附近由于水深增加导致余流减小2~7 cm/s。莱州湾黄河口附近的逆时针环流向东南方向移动,黄河口北部余流略有减小,东南部余流明显增大,增加量最多能达到9 cm/s。刁龙嘴南侧顺时针环流减小,北侧顺时针环流增大4~9 cm/s。