STUDIES ON IMMUNODIAGNOSIS OF HEPATOPANCREATICPARVO-LIKE VIRUS DISEASES OF PENAEUS ORIENTALISII. RAPID EARLY DIAGNOSIS

This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory results.This is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid shrimp.The SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms.     

Studies on immunodiagnosis of hepatopancreatic parvo-like virus disease of the Chinese penaeid,Penaeus Chinensis I. Purification of the virus

The viral discase of penaeids is indistinet but highly dangerous because their symptoms are often masked by some secondary ones. In order to gain knowledge on how to prevent the occurrence and spread of this viral disease, diagnostic studies on the early phase of the hepatopancreatic parvo-like viral (HPV) disease of culturedPenaeus chinensis was conducted using ummuno-serological techniques. The purification of HPV was successfully done by density gradient ultracentrifugation of cane sugar.     

The prediction of penaeid shrimp yield in the Bohai Sea in autumn

This project was designed to predict penaeid shrimp yield in the Bohai Sea in autumn by means of multiple regression equations based on the data of abundance index obtained from the trawl surveys in the main spawning ground of shrimp in the Bohai Sea and the nursery grounds of juvenile shrimp (Bohai, Laizhou and Liaodong bays and the Luanhe River estuary) in early August every year from 1965–1982. The prediction method and its use are presented in this paper. The abundance indexes obtained from the four sea areas as four independent variables, when subjected to an F test, showed that they had significant effects on the penaeid shrimp yield in the Bohai Sea in autumn.     

On the 2-crop culture of penaeid shrimps

Although much progress has been made in the last few years on the hatchery rearing of penaeid shrimps, the use of artificial feeds and the innovation of culture techniques, the key problem in the shrimp culture industry is still to increase yield per unit culture area for greater economic returns. In finding an answer to this problem, experiments on the cultivation of two (instead of one) species with different biological requirements were carried out in different seasons,Penaeus orientalis Kishinouye in spring andP. penicillatus Alcock in summer in Fujian as it has a longer culture season. Years of study and practical experience in large-scale rearing of shrimps show that successive 2-crop culture of penaeid shrimps is feasible and more economical as no additional investment in terms of maintenance facilities and management is needed. The pond is fully used the whole year. The renovation of techniques and the timing of the culture of the two species led to the development of a 2-crop culture method which roughly doubled the yield per unit area of cultivation. The early season species is harvested at the prime of its development and the culture of the late season species begin just after the harvest of the early season species.     

Ontogenic study of isozymes in giant tiger prawn,Penaeus monodon (Crustacea: Decapoda)

The patterns of isozyme variation throughout the life cycle of the giant tiger prawn,Penaeus monodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH, PGD, and PROT). The zymograms showed little variation in the number of isozymic loci expressed during larval development compared with results reported for other penaeid prawns. There were specific ontogenic patterns at some loci. As larvae developed beyond the embryonic stage, there was a tendency for intensity of staining and complexity of banding patterns to increase.     

STUDIES ON IMMUNODIAGNOSIS OFHEPATOPANCREATIC PARVO- LIKEVIRUS DISEASE OF THE CHINESEPENAEID, PENAEUS CHINENSISI. PURIFICATION OF THE VIRUS

The viral disease of penaeids is indistinct but highly dangerous because their symptoms are often masked by some secondary ones.In order to gain knowledge on how to prevent the occurrence and spread of this viral disease, diagnostic studies on the early phase of the hepatopancreatic parvo-like viral (HPV)disease of cultured Penaeus chinensis was conducted using immuno-serological techniques. The purification of HPV was successfully done by density gradient ultracentrifugation of cane sugar.     

一种抗简单功耗分析攻击的椭圆曲线标量乘快速实现算法

针对添加伪点加法在抵抗简单功耗分析攻击的同时效率损失过大的问题,为更好兼顾效率与安全,提出一种新的标量乘快速实现算法——随机伪操作法,通过以单片机为核心运算控制芯片的功耗分析平台进行实测分析验证,随机伪操作法不但能够很好抵抗简单功耗分析攻击,而且相对于添加伪点加法运算效率提高30%-50%。     

一种基于决策者满意度的多属性决策方法

提出一种基于决策者满意度的多属性决策方法.分别给出构造定量指标的基于决策者满意度的满意度函数模型和定性指标的集对满意度函数模型.以某保险公司为例,对其分公司培训部培训绩效进行综合评价和结果分析,并讨论了该决策方法的优点.     

Debris flow hazard assessment using set pair analysis models: Take Beichuan county as an example

Assessment of debris flow hazards is important for developing measures to mitigate the loss of life and property and to minimize environmental damage. Two modified uncertainty models, Set Pair Analysis （SPA） and modified Set Pair Analysis （mSPA）, were suggested to assess the regional debris flow hazard. A ease study was conducted in seven towns of the Beichuan county, Sichuan Province, China, to test and compare the application of these two models in debris flow hazard assessment. The results showed that mSPA only can fit for value-variables, but not for non value-variable assessment indexes, Furthermore, as for a given assessment index xi, mSPA only considers two cases, namely, when grade value increases with xi and when grade value decreases with xi. Thus, mSPA can not be used for debris flow hazard assessment but SPA is credible for the assessment because there are no limitations when using SPA model to assess the debris flow hazard. Therefore, in this study SPA is proposed for assessing debris flow hazard.     

Impact of Vibrio parahaemolyticus and white spot syndrome virus (WSSV) co-infection on survival of penaeid shrimp Litopenaeus vannamei

White spot syndrome virus(WSSV) is an important viral pathogen that infects farmed penaeid shrimp, and the threat of Vibrio parahaemolyticus infection to shrimp farming has become increasingly severe. Viral and bacterial cross or superimposed infections may induce higher shrimp mortality. We used a feeding method to infect L itopenaeus vannamei with WSSV and then injected a low dose of V. parahaemolyticus(WSSV+Vp), or we fi rst infected L. vannamei with a low-dose injection of V. parahaemolyticus and then fed the shrimp WSSV to achieve viral infection(Vp+WSSV). The effect of V. parahaemolyticus and WSSV co-infection on survival of L. vannamei was evaluated by comparing cumulative mortality rates between experimental and control groups. We also spread L. vannamei hemolymph on thiosulfate citrate bile salt sucrose agar plates to determine the number of V ibrio, and the WSSV copy number in L. vannamei gills was determined using an absolute quantitative polymerase chain reaction(PCR) method. L v My D88 and Lvakt gene expression levels were detected in gills of L. vannamei by real-time PCR to determine the cause of the different mortality rates. Our results show that(1) the cumulative mortality rate of L. vannamei in the WSSV+Vp group reached 100% on day 10 after WSSV infection, whereas the cumulative mortality rate of L. vannamei in the Vp+WSSV group and the WSSV-alone control group approached 100% on days 11 and 13 of infection;(2) the number of Vibrio in the L. vannamei group infected with V. parahaemolyticus alone declined gradually, whereas the other groups showed signifi cant increases in the numbers of Vibrio( P 0.05);(3) the WSSV copy numbers in the gills of the WSSV+Vp, Vp+WSSV, and the WSSV-alone groups increased from 10 5 to 10 7 /mg tissue 72, 96, and 144 h after infection, respectively. These results suggest that V. parahaemolyticus infection accelerated proliferation of WSSV in L. vannamei and vice versa. The combined accelerated proliferation of both V. parahaemolyticus and WSSV led to massive death of L. vannamei.     

Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis): a chromosome-specific marker for chromosome identification

Chinese shrimp(Fenneropenaeus chinensis)is an economically important aquaculture species in China.However,cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze.In this study,fluorescence in-situ hybridization(FISH) was used to identify the chromosomes of F.chinensis.The 5S ribosomal RNA gene(rDNA)of F. chinensis was isolated,cloned and then used as a hybridization probe.The results show that the 5S rDNA was located on one pair of homologous chromosomes in F.chinensis.In addition,triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method.It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F.chinensis.The successful application of FISH in F.chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps.     

A study on pathogens of Chinese prawn (Penaeus Chinensis) virus diseases

This pathogenic study shows that the viral diseases of Chinese prawns (Penaeus chinensis, O'sbeck) is due to three kinds of viruses: epithelium envelope baculovirus ofPenaeus chinensis (EEBV-PC, detected by the authors in 1993), infections hypodermal and hematopoietic necrosis virus, and hepatopancreatic parvo-like virus, and that the first two viruses seem to be the main pathogens of the epidemic in the northern regions in 1993.     

ONTOGENIC STUDY OF ISOZYMES IN GIANT TIGER PRAWN,PENAEUS MONODON (CRUSTACEA:DECAPODA)

The patterns of isozyme variation throughout the life cycle of the giant tiger prawn, Penaeusmonodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH,PGD, and PROT). The zymograms showh little variation in the number of isozymic loci expressed duringlarval development compared with results reported for other penaeid prawns. There were specific ontogenicpatterns at some loci. As larvae developed beyond the embryonic stage, there was tendency for intensityof staining and complexity of banding patterns to increase.     

一种抗简单功耗分析攻击的模幂算法

在真实环境下对智能卡RSA算法进行了功耗采集,通过简单功耗分析攻击（SPA）提取出了密钥,证明了传统的模幂运算二元表示（BinaryRepresentation,BR）算法存在明显的密钥信息泄露缺陷。分析发现,信息泄露发生在程序的跳转分支处。针对这一缺陷,提出了一种原子化操作与引入随机数相结合的模幂算法。通过操作的原子化统一了模幂算法的执行路径,消除因跳转分支引起的信息泄露;引入随机数对密钥的汉明重量进行掩盖,有效地防止密钥信息的泄露。在MC8051上的实测验证表明,新算法确实具有抗简单功耗分析的特性。     

Seasonal variation in imposex intensity of Thais clavigera

Imposex, specifically caused by TBT pollution, refers to the superimposition of male sexual characteristics in gastropod females. Seasonal variation of imposex intensity inThais clavigera from both slightly and severely contaminated sites in Hong Kong waters was studied from 1988 to 1999. The male penis length showed significant difference between both sites and seasons. It was shortest during late autumn and early winter (October to December) and longest during spring and early summer (February to June). Female penis length also showed significant difference between sites. It did not change seasonally, however. The RPS (Relative Penis Size) index was the highest during autumn and early winter, and the lowest during spring and early summer. The VDS (Vas Deferens Sequence) index remained stable throughout the sampling period. This study showed that VDS index is a better indicator when we compare relative intensity of imposex. The comparison can only be meaningful provided the samples from different locations are taken during the same season. Supported by the NSFC (40006007).     

Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis): a chromosome-specific marker for chromosome identification

Chinese shrimp (Fenneropenaeus chinensis) is an economically important aquaculture species in China. However, cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze. In this study, fluorescence in-situ hybridization (FISH) was used to identify the chromosomes of F. chinensis. The 5S ribosomal RNA gene (rDNA) of F. chinensis was isolated, cloned and then used as a hybridization probe. The results show that the 5S rDNA was located on one pair of homologous chromosomes in F. chinensis. In addition, triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method. It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F. chinensis. The successful application of FISH in F. chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps.     

Viral Diseases of Olive Flounder in Korean Hatcheries

In order to elucidate the state of diseases, especially viral diseases, and to prevent viral diseases from occurring in olive flounder hatcheries, a range of studies, including epidemiological study, were performed from 1997 to 2003. The location of the hatcheries investigated includes several representative sites in the east （Kangnung, Uljin, Pohang, Yangsan, Ulsan, Pusan）, south （Wando, Changheung, Goheung, Yeosu, Namhae, Tongyeong, Geoje, Jeju） and west （Seosan, Kunsan, Gochang, Yeongkwang, Mokpo, Chindo） costal areas of the Korea Peninsula. A total of 2000 cases have been examined in 7 years, in which mortality caused by viral agents accounts for 22%, or 446 cases. Mortalities associated with viral infection considerably increased from 14% in 1997 to 27% in 2003. A variety of viral diseases were observed, and the occurrences of viral epidermal hyperplasia, viral ascites and viral deformity, viral nervous necrosis, and hirame rhabdoviral disease are 14%, 51%, 25%, and 8% respectively. By investigating the viral infection of broodstock flounder, the infection rate of marine birnavirus （MABV） in hatcheries was identified to be approximately 30%, therefore, it is highly necessary to acquire and keep non-infected broodstock fishes.     

海洋鱼类疾病诊断系统中的多参数的证据不确定性推理机制

在分析、归纳、总结不确定性推理相关问题的基础上,提出基于充分性参数、必要性参数和可信度因子(规则强度)等多参数的证据不确定性推理方法。该方法能够表示具有复杂不确定性加权关系的诊断规则,并且能够根据证据规则的约束条件通过数值计算获取规则的不确定性相关参数;通过在开发的海洋鱼类疾病诊断专家系统中的应用,很好地体现了疾病诊断中存在的不确定性,有效地减少了主观因素对诊断结论准确性的影响。     

针对抗边信道攻击的椭圆曲线标量乘法的简单功耗分析

分析了一种运用平衡点加一倍点操作抗边信道攻击的椭圆曲线标量乘法。方法虽然使点加和倍点的功耗不可区分,但是当标量用二进制表示时,标量乘法的运算功耗仍有边信息泄露。对标量乘算法的传统功耗攻击主要集中在点加运算和倍点运算之间的功耗差异上,文中将攻击点转移到标量乘算法中for语句和if语句之间的功耗差异上,并成功对平衡点加一倍点操作后的标量乘功耗曲线实现了简单功耗攻击。     

Isolation of plasmid from the blue-green algaSpirulina platensis

CCC plasmid was isolated from an economically important blue-green alga —Spirulina platensis (1.7×10⁶ dalton from the S₆ strain and 1.2×10⁶ dalton from the F₃ strain) using a rapid method based on ultrasonic disruption of algal cells and alkaline removal of chromosomal DNA. The difference in the molecular weight of the CCC DNAs from the two strains differing in form suggests that plasmid may be related with the differentiation of algal form. This modified method, which does not use any lysozyme, is a quick and effective method of plasmid isolation, especially for filamentous blue-green algae. Contribution No. 79 of the Experimental Marine Biology Laboratory and 2153 of the Institute of Oceanology, Academia Sinica. This research was supported in part by The President of the Chinese Academy of Sciences Foundation.