Fish Assemblages of Posidonia oceanica Beds at Port-Cros (France, NW Mediterranean): Assessment of Composition and Long-Term Fluctuations by Visual Census

Abstract. Observations of the fish fauna of Posidonia oceanica (L.) DELILE seagrass beds were undertaken in two shallow stations and one deeper station in Port-Cros National Park, between November 1984 and September 1987. Demographic structure, density and biomass of the 19 most abundant species or taxa were assessed by several non-destructive sampling methods. The fish assemblages studied were dominated in number and biomass by a small number of species (Labridae: Symphodus ocellatus and S. tinca ; Sparidae: Diplodus annularis and Sarpa salpa ). Large individuals were infrequent and small size class fish were dominant especially in sheltered shallow areas. Recruitment and migrations (shortor long-term) have been identified as the most important causes of abundance variations over the year. The four trophic categories were sampled only in the sheltered shallow zone, where the herbivore Sarpa salpa represents a strong proportion of the fish fauna (40–70%) during summer. The fish assemblages differ between the three seagrass beds, and the depth appears as the most important factor affecting the fish assemblages in P. oceanica . The functional difference between shallow and deeper seagrass beds is therefore discussed.     

A Map of the Posidonia oceanica Beds of Marina ďElbu (Corsica, Mediterranean)

Abstract. A large-scale map of the Posidonia oceanica seagrass beds as well as other benthic substrata and communities was compiled for a cove of a marine reserve free from pollution and man-made disturbance. An extensive diving survey of the cove and the interpretation of aerial colour photographs were used. The greater part of the cove is occupied by the P. oceanica meadow. Patches of dead "matte" were observed at a depth of between 7 and 14 m. They may be the result of a natural hydrodynamic equilibrium between building and erosion.     

Organic Matter Derived from a Seagrass Meadow: Origin, Properties, and Quality of Particles

Abstract. An investigation of the properties of particles found in wrack beds and suspended in the water body of a Posidonia oceanica system led to the following conclusion: except for the period of the leaf fall and at times of strong water movement (e. g. winter storms), the majority of the particles in the water show no similarity with seagrass particles. This is based on the comparison of C: N ratios and SEM inspection. Information on the magnitude of DOC fluxes in this system led to the formulation of the phase shift hypothesis from DOM to POM. Because wrack particles were shown to have relatively fast settling rates, it is expected that processes like adsorption onto inorganic particles, micellation and aggregate formation have more importance in regulating particle density in the water body than the resuspension of directly fractionated Posidonia debris. The implication of these findings are discussed and the importance of the Mediterranean seagrass P. oceanica as an energy source supporting secondary production in adjacent ecosystems is questioned.     

Heavy Metal Contents in Soft-Bottom Marine Macrophytes and Sediments Along the Mediterranean Coast of Spain

Abstract. Hg, Cd, Pb and Zn concentrations were determined in sediment and in tissues of five species of soft-bottom marine macrophytes ( Posidonia oceanica, Cymodocea nodosa, Zostera noltii, Ruppia cirrhosa and Caulerpa prolifera ) along the Spanish Mediterranean coast. Levels of metals were low in most of the sampling stations and similar to those found by other authors in uncontaminated zones. Certain locations, however, showed some degree of contamination (Cambrils, Almassora, Alacant, Mar Menor and El Portús). In Santa Pola we found high contents of metals in one sample of sediment due to the high proportion of the fine fraction (particules < 63µm) and organic matter, but not in the seagrass species. Mercury and zinc concentrations in the sediment are correlated to those in at least some anatomic fractions of Posidonia oceanica, Cymodocea nodosa and Caulerpa prolifera , suggesting that these species reflect the levels of these metals in the environment. Lead contents in the alga Caulerpa prolifera are also correlated to those in the sediment, while no significant correlations were obtained for cadmium. Among the species studied, Posidonia oceanica and Cymodocea nodosa seem to be better biomonitors than Caulerpa prolifera .     

Antioxidant response of the seagrass Posidonia oceanica when epiphytized by the invasive macroalgae Lophocladia lallemandii

The aim was to evaluate the antioxidant defences and the occurrence of oxidative damage in Posidonia oceanica under a stress situation due to the epiphytism of the invasive macroalgae Lophocladia lallemandii. P. oceanica leaves were collected in the absence (control station) and in the presence of the epiphytic algae L. lallemandii and the antioxidant enzyme activities, markers of oxidative damage, and hydrogen peroxide production were determined. Antioxidant enzyme - catalase, glutathione peroxidase and superoxide dismutase - activities were significantly higher in Posidonia epiphytized by L. lallemandii. Malondialdehyde, protein carbonyl derivates, and glutathione levels were also higher in L. lallemandii epiphytized P. oceanica leaves compared to control samples. The production of hydrogen peroxide was also significantly increased when Posidonia was epiphytized by L. lallemandii. The invasion of P. oceanica meadows by L. lallemandii appeared to induce oxidative stress in the seagrass as evidenced by increased levels of oxidative stress markers and antioxidant defences.     

Cyclic Changes along Posidonia oceanica Rhizomes (Lepidochronology): Present State and Perspectives

Abstract. Leaf sheaths in the seagrass Posidonia oceanica can persist for millcnia and they show, according to their insertion rank, cyclic variations in parameters such as thickness and tissue structure. The principal cycles are always annual, but pluriannual cycles can also be detected. Annual cycles may be changed by environmental parameters such as light, temperature, or water movement. In a fashion analogous to the study of annual tree rings (dendrochronology), the study of cyclic changes in Posidonia oceanica sheaths is thus a technique allowing the study of past yearly changes in the near-shore environment.     

The Detritic Compartment in a Posidonia oceanica Meadow: Litter Features, Decomposition Rates, and Mineral Stocks

Abstract. The ecosystem associated to the Mediterranean seagrass Posidonia oceanica shows a clear distinction in two subcompartments regarding turnover time: aboveground and belowground. Aboveground parts (leaves) are highly dynamic, and most of the leaf material is decomposed or exported in less than one year, representing a net loss of nutrients. In contrast, belowground biomass (roots and rhizomes) has a turnover time of the order of centuries, with a consequent accumulation of organic matter in the sediment. The accumulation rates for the single elements rank in the order C > N > P. This ecosystem may be considered as a sink for biogenic elements.     

Trawling or Visual Censuses? Methodological Bias in the Assessment of Fish Populations in Seagrass Beds

Abstract. Fishes associated with the seagrass Posidonia oceanica were censused both by skid trawl and visual counts around Ischia Island, Italy. Both sampling procedures were performed during the day in summer and in winter at two sites. Significant differences in the number of species, number of individuals, biomass, and trophic structure of the fish assemblage were observed between sampling methods. Fewer fish species were recorded by visual counts than by trawling. More individuals and a greater biomass, however, were recorded from visual counts. The population of macrocarnivores (Scorpaenidae, Serranidae) were better estimated by trawling, as were canopy-dwellers (Syngnathidae; Symphodus rostratus) and benthic species (Gobiidae; Blenniidae; Bothidae). Conversely, good swimmers ( Sparidae; Coris julis, Symphodus spp.), and planktivorous fishes (Centracanthidae, Pomacentridae) mostly escaped the trawl and were better assessed by visual counts. The importance of methodological biases differed from one season to the other and was higher in summer than in winter. Particular attention should be paid to the biases induced by sampling techniques when interpreting data, and different sampling methods should be used to accurately study the fish assemblages of seagrass meadows.     

Accumulation of periphyton biomass on artificial substrates deployed near a sewage sludge outfall in South Australia

Digested sludge from the Port Adelaide Sewage Treatment Works has been discharged into Gulf St Vincent, a marine embayment in South Australia, since 1978. The outfall was sited in seagrass meadows and four years after it began operating the dominant seagrass species were lost from an area of 365 ha. Discernible effects extended over 1900 ha and the seagrass beds in partially affected areas were characterized by unusually heavy growth of epiphytes. Artificial substrates were used to assess the accumulation of ‘epiphyte’ (periphyton) biomass in areas affected by sludge and at a control site. The greatest accumulation was recorded in the area most affected by sludge, i.e. the area from which all seagrass has been lost. It is suggested that excessive accumulation of epiphytes, resulting from the addition of sludge to the water column, may have contributed to the decline of the original seagrass beds.     

Variability in the structure of epiphytic assemblages of the Mediterranean seagrass Posidonia oceanica in relation to depth

The aim of the present study was to evaluate whether the variability in the structure of the epiphytic assemblages of leaves and rhizomes of the Mediterranean seagrass Posidonia oceanica differed between depths at a large spatial scale. A hierarchical sampling design was used to compare epiphytic assemblages at two different depths (10 and 20 m) in terms of both species composition and abundance and patterns of spatial variability in the Tuscan Archipelago (North Western Mediterranean Sea, Italy). Results showed significant differences in the structure of assemblages on rhizomes and leaves at different depths. These differences were related to species composition and abundance; differences were not significant for total biomass, total percentage cover and percentage cover of animals and algae. Whereas the higher variability was observed among shoots in all the studied systems, patterns of spatial variability at the other spatial scales investigated differed between the two studied depths. Moreover, in the present study, analogous patterns between depths resulted for both the assemblages of leaves and rhizomes, suggesting that factors that change with depth can be responsible for the spatial variability of both the assemblages (leaves and rhizomes), and operate regardless of the microclimatic conditions and the structure of assemblages.     

Comparison of the fish assemblages associated with Posidonia oceanica after the partial loss and consequent fragmentation of the meadow

An extensive Posidonia oceanica meadow was partially destroyed by excavation, resulting in areas of seagrass habitat of equal complexity (shoot density) but different heterogeneity (degree of fragmentation). The fish assemblages associated with differently fragmented beds were compared from a landscape perspective. Differences in the fish assemblages were detected, with several species showing different patterns: (1) species that increased their abundance along with the degree of bed fragmentation, (2) species that were more abundant in fragmented beds, but did not show differences between more or less fragmented beds, and (3) species that were mostly abundant in large seagrass patches or in the continuous meadow. The fish assemblages were also affected by depth, but further research is needed to determine properly this effect. Some ecological mechanisms are suggested to operate in the interaction between P. oceanica and the held fish assemblage.     

Polychaete Depth Distribution in a Posidonia oceanica Bed (Rhizome and Matte Strata) and Neighbouring Soft and Hard Bottoms

Abstract. The study was carried out in a Posidonia oceanica bed and in the neighbouring sandy and rocky bottoms, at Ponza Island (Central Tyrrhenian Sea), in order to investigate the distributional pattern of the polychaete community at different depths. A total of 15232 individuals of polychaetes belonging to 218 species was collected and three new species of Syllidae were found. The polychaete community of the matte can be clearly distinguished from the neighbouring communities; this is due to the high species richness in Posidonia , which is related to the heterogeneity of the biotope. In the Posidonia bed the shallow stations (1 m and 5 m depth) were the richest in terms of species numbers and individuals due to the high shoot density of the seagrass bed. In the shallow waters polychaete community structure changed according to the different exposure to water movement. Rocky bottom species, which preferentially inhabit crevices, were more abundant in the matte exposed to water movement (5–10 m depth). In contrast, soft-bottom species were more abundant in the sheltered zone (1 m depth) as well as in the deeper zone due to the reduction of the Posidonia bed.     

Short-term response of the slow growing seagrass Posidonia oceanica to simulated anchor impact

Experimental evaluations about the impact of anchors of small vessels have previously shown that each anchoring can on average damage up to six shoots of Posidonia oceanica, removing small amount of biomass and, at the same time, interrupting continuity among shoots. The aim of the paper was to investigate the response of P. oceanica to different damage intensity at two levels of substrata compactness. Three treatments were considered: control (no damage); low damage (simulated anchor damage by three strokes of a hoe); and high damage (six strokes). Disturbance was higher where the substratum was highly penetrable and after one year significant variation was observed among treatments for both the number of leaves per shoot and shoot density. Conversely, the number of leaves per shoot by the end of the study was similar among all treatment combinations, suggesting that this was the only phenological feature that recovered, and probably the only result that through clonal integration could be achieved. These data strongly highlighted the role that anchoring might have on the slow growing seagrass P. oceanica meadow.     

Systematics and Ecology of the Hydroid Population of two Posidonia oceanica Meadows

Abstract. The ecology and systematics of the hydroid population of Posidonia oceanica meadows have been studied in two different zones of the Western Mediterranean. A new sampling method has been employed and the results obtained have been compared with those of a standard method. The distribution of the species and several morphological variations are pointed out. Depth-related faunistic zones are evidenced. The key-species of the Posidonia hydroid population arc individuated.     

Photo-library method for mapping seagrass biomass

The creation of seagrass biomass maps by diving/snorkelling is time-consuming and expensive. This paper presents a method for estimating seagrass dry weight using a photo-library of classes of differing seagrass biomass. Field data were collected at seagrass beds in Ngederrak Reef, Palau, in 2006. Photos of 25 × 25 cm quadrats were taken prior to the collection the above-ground biomass for determination of biomass dry weight. Fifteen classes of seagrass biomass and substrate type were identified. The dry weight for each class of seagrass was measured in laboratory. A photo-library was created for biomass classification where each in situ quadrat photo is accompanied with seagrass dry weight of the sample and a photo of the sorted sample taken in laboratory. The photo-library of quadrats was then used to estimate seagrass biomass on photos gathered along 100 m long transects at 2 m intervals. This procedure was conducted by three different observers. The seagrass dry weight estimates were consistent between interpreters even if one of the interpreters had no experience in seagrass research. This approach allows quick collection of seagrass dry weight data over large areas. The method can be used for creating seagrass biomass maps by snorkelling/diving and/or for calibrating and validating biomass maps created by remote sensing.     

The Importance of Posidonia oceanica and Cymodocea nodosa as Contributors of Free Amino Acids in Water and Sediment of Seagrass Beds

Abstract. Dissolved free amino acids (DFAA) were measured in sediment porewater and overlying sea water in seagrass beds of Posidonia oceanica and Cymodocea nodosa at a water depth of 8–10 m in Corsica, France. In the sea water, DFAA concentrations from 0.5 to 1.9μM were found. Lowest concentrations occurred in the afternoon in surface water samples, while higher concentrations were measured in morning surface samples and in all bottom samples. The most abundant amino acids were serine, glycine, and alanine. In the sediment, the upper 4cm contained the highest concentrations of DFAA (177nmol cm^-3 or 367 μM), while lower concentrations (<50nmol cm^-3 or 104 μM) prevailed deeper in the sediment. Glycine, glutamic acid, alanine, and β-alanine were the major components of the DFAA. The photosynthetic activity of the seagrasses influenced sediment porewater pools of DFAA. When a Posidonia bed was covered with a black polyethylene shield, the interstitial amino acids were reduced at a rate of 16nmol cm^-2 h^-1 (0-10cm depth). Conversely, extended daylight (floodlight) increased the DFAA pool in sediment depths below 4 cm. Free amino acids were released to the overlying sea water from both seagrass sediment and individual shoots, the rates being highest in the dark. The present investigation indicates that a substantial portion of the N-cycling of the seagrass sediment includes free amino acids.     

Zonation and Ecology of Epiphytic Hydroids in a Mediterranean Coastal Lagoon: The 'Stagnone' of Marsala (North-West Sicily)

Abstract. Distribution patterns of epiphytic hydroids of seagrass ( Posidonia occanica and Cymodocea nodosa ) beds were studied in the 'Stagnone', a moderately hypcrhaline lagoon in Sicily. The role of water movement in determining the spatial and temporal zonation of epiphytic hydroids and their morpho-physiological variations is evaluated. Remarks on systematics arc also included.     

海南典型热带海草床4种代表性鱼类的生长特征及其对海草资源量变化的响应

文章以海南岛新村湾、黎安港和潭门港海草床为研究区域, 选取褐篮子鱼(Siganus fuscessens)、点斑篮子鱼(Siganus guttatus)、四带牙鯻(Pelates quadrilineatus)和细鳞鯻(Terapon jarbua) 4种代表性鱼类为研究对象, 分析其体长与体质量的关系, 并探讨4种鱼类对海草资源量变化的响应。结果表明, 除栖息于潭门港海草床的4种鱼为负异速生长, 其他区域海草床的鱼类均为正异速或等速生长; 4种鱼的体质量生长速率(即异速生长因子b)和肥满度呈现黎安港>新村湾>潭门港的趋势, 并与3个海草床的海草覆盖率、密度和生物量的变化趋势一致; 推测海草资源量下降可能增加鱼类的被捕食压力和减少食物来源, 从而导致生活于海草床的代表性鱼类生长速率下降。     

Biometric and Genetic Analysis of the Marine Rissoid Rissoa auriscalpium (Gastropoda, Prosobranchia) and its Ecological Implications

Abstract. Two forms of Rissoa auriscalpium were found in Posidonia oceanica beds around the Island of Ischia (Gulf of Naples). In order to investigate their taxonomical value and to quantify the discriminating features found, the radular, conchometric, genetic, and ecological features of the two forms were analyzed. Ecological observations showed that the distribution of the two forms is strongly correlated with hydrodynamic factors.     

Effects of current exposure on habitat preference of mobile 0-group epibenthos for intertidal seagrass beds (Zostera noltii) in the northern Wadden Sea

In the northern Wadden Sea, the extent of intertidal seagrass beds, their plant biomass and shoot density highly depends on local current regimes. This study deals with the role of intertidal Zostera noltii beds as nursery for mobile epibenthic macrofauna and the impact of seagrass bed characteristics on their abundance and distribution patterns. According to their exposure to the main tidal gullies, sampling sites were separated into exposed, semi-exposed and sheltered. Dominant species of crustaceans and demersal fish were studied in respect of their abundances within seagrass beds and adjacent unvegetated areas. Quantitative sampling was performed at day and night high tide using a portable drop trap. In general, species composition varied little between seagrass beds and bare sand. However, the presence of vegetation had a quantitative effect increasing individual numbers of common epifaunal species. Abundances of 0-group shore crabs (Carcinus maenas), common gobies (Pomatoschistus microps) and brown shrimps (Crangon crangon) were highest within sheltered seagrass beds. With decreasing plant density habitat preference of epibenthos changed on species level. By regulating the habitat complexity the currents regime is profoundly influencing the nursery function of intertidal seagrass beds in the Wadden Sea.