Aquatic macrophyte dynamics in Lake Karakul (Eastern Pamir) over the last 29 cal ka revealed by sedimentary ancient DNA and geochemical analyses of macrofossil remains

Due to methodological challenges there are only a few studies that focus on macrophyte dynamics in large lakes despite their notable role in a lake’s ecosystem functioning. This study investigates composition and productivity changes of the submerged vegetation of Lake Karakul, Pamir Mountains (Tajikistan), using sedimentary ancient DNA metabarcoding and elemental (C/N) and isotopic (δ¹³C, δ¹⁵N) measurements of Stuckenia cf. pamirica (Baagøe) Z. Kaplan (Potamogetonaceae) leaf remains. No Stuckenia cf. pamirica leaf remains were found for 28.7–26.1 cal ka BP, when both Potamogetonaceae and Chara (L.) DNA sequences were recorded, suggesting sparse submerged vegetation at the coring site. This agrees with the inference of a deep lake reached using geochemical proxies. From 26.1 to 17.5 cal ka BP a few macrophyte remains and high numbers of Potamogetonaceae sequences were recovered: lake level was probably low, as suggested by other studies on the lake. Another phase of increased numbers of Chara sequences and the absence of Stuckenia cf. pamirica leaf remains was found between 17.5 and 12.2 cal ka BP, which coincides with a lake-level transgression at Lake Karakul as indicated by paleo-shoreline investigations. Analyses of macrophyte remains reveal intermediate paleo-productivity from 6.9 cal ka BP and high paleo-productivity from 2.2 cal ka BP onwards. From comparisons with other studies, we suggest that lake-level changes are the main driver for the submerged vegetation composition and productivity at the coring site in Lake Karakul and underline our conclusions by depicting the present-day distribution of Stuckenia cf. pamirica and Chara within the lake.     

Sclerochronological oxygen and carbon isotope ratios in Radix (Gastropoda) shells indicate changes of glacial meltwater flux and temperature since 4,200 cal yr BP at Lake Karakul,eastern Pamirs (Tajikistan)

We report δ¹⁸O and δ¹³C values of 21 fossil shells from the aquatic gastropod Radix from a sediment core taken in the eastern basin of Lake Karakul, Tajikistan (38.86–39.16°N, 73.26–73.56°E, 3,928 m above sea level) and covering the last 4,200 cal yr BP. The lake is surrounded by many palaeoshorelines evidencing former lake-level changes, most likely triggered by changes in meltwater flux. This hypothesis was tested by interpreting the isotope ratios of Radix shells together with δ¹⁸O values of Ostracoda and of authigenic aragonite. The mean δ¹⁸O values of Radix and Ostracoda fall along the same long-term trend indicating a change in the isotopic composition of precipitation, which contributed to the glaciers in the catchment as snow and finally as melt water to the lake. The sclerochronological δ¹⁸O and δ¹³C patterns in Radix shells provide seasonal weather information, which is discussed in context with previously proposed climatic changes during the last 4,200 cal yr BP. The period between ~4,200 and 3,000 cal yr BP was characterized by stepwise glacier advance in the catchment most likely due to a precipitation surplus. Subsequently the climate remained relatively cold but the lake level fluctuated, as indicated by ostracod shell isotope data. From ~1,800 cal yr BP the sclerochronological patterns provide evidence for increasing melt water flux and transport of allochthonous carbon into the lake, most likely due to an accelerated glacier retreat. The period around 1,500 cal yr BP was characterized by strong warming, increasing meltwater flux, glacier retreat and an increasing lake level. Warm conditions continued until ~500 cal yr ΒP probably representing the end of the Medieval Warm Period. A short relatively cold (dry?) period and a lower lake level are assumed for ~350 cal yr BP, possibly an analogue to the Maunder Minimum cooling in the North Atlantic region. Our results show that the lake system is complex, and that changes were triggered by external forcing and feedbacks. The similarity of δ¹⁸O values in Radix and ostracod shells demonstrates that both archives provide complementary information.     

Diatom responses to environmental changes in the Upper Paraná River floodplain (Brazil) during the last ~ 1000 years

The floodplain of the Upper Paraná River, Brazil, is strongly influenced by hydrology, which in turn affects geomorphological and environmental conditions, and controls the form of islands in the river. Such islands develop by deposition of river-borne sediment that creates small lateral sediment bars. Geomorphological processes can produce a variety of aquatic environments on such islands, e.g. channels, backwaters, lakes, transitional areas, and swamps. Our objective was to test whether subfossil diatoms preserved in the sediment on an island in the Upper Paraná River floodplain responded to changes in limnological conditions brought about by such geomorphological modifications. We hypothesized that the composition of diatom assemblages in the sediment shifted in response to past geomorphic, and hence limnological conditions. We analyzed diatom subfossils in a 2-m-long sediment core with a calibrated date near the base of 1047–1224 cal yr AD. Absence of diatoms at the bottom of the sequence was associated with the channel phase, followed by appearance of diatoms 1229–1381 cal yr AD that were adapted to flow, in the backwater phase. After another 100–200 years, presence of Eunotia species in the lake phase suggests a decrease in pH, phosphorus and nitrogen. Replacement of Eunotia spp. by Diadesmis species, following a transition phase, suggests different environmental conditions, with reduced water depth. Diatoms in surface deposits are distinct from assemblages in the other phases in the core and contain taxa that suggest a disturbed environment, with variations in water depth and flow. The data illustrate the importance of physical and hydrological factors in shaping diatom communities and show the utility of diatoms as bioindicators in this floodplain environment.     

Lake evolution and hydroclimate variation at Lake Qinghai (China) over the past 32 ka inferred from ostracods and their stable isotope composition

We used ostracod species assemblages and their δ¹⁸O values in a 32-m sediment core from Lake Qinghai, China, along with information from cores collected at other sites in the lake, to infer lake evolution and hydroclimate changes since the last glacial. Dominant ostracod species Ilyocypris bradyi and its low δ¹⁸O values showed that Lake Qinghai was small in size or even consisted of several playa lakes, and the 1F core site could have even been in a wetland setting, under cold and dry climate conditions before 15.0 ka. Presence of Limnocythere inopinata with low δ¹⁸O values, and absence of I. bradyi after 15.0 ka, indicate the lake area increased or that the playas merged. The decrease or disappearance of ostracods with high δ¹⁸O values showed that the lake shrunk under dry climate from 12.0 to 11.6 ka. After 11.6 ka, hydroclimate shifts inferred from ostracod species changes (Eucypris mareotica and L. inopinata) and their δ¹⁸O values were as follows: (1) 11.6–7.4 ka—larger, but still small lake area with greater moisture availability under primarily dry climate conditions, (2) 7.4 to 3.2 ka—increasing lake level under a warmer and wetter climate, and (3) 3.2 ka to present—stable, large, brackish lake. The low ratio of lake water volume to runoff, and close proximity of the core site to freshwater input from the river mouth would have resulted in relatively lower ostracod δ¹⁸O values when Lake Qinghai was small in area during the interval from 32.0 to 15.0 ka. Lower ostracod δ¹⁸O values during interstadials and throughout the entire Last Glacial Maximum and early deglacial (ca. 24.0–16.0 ka) were caused by a greater contribution of seasonal meltwater from ice or snow and low incoming precipitation δ¹⁸O values related to cold climate conditions in the region at that time.     

Water level changes in Lake Van,Turkey, during the past ca. 600 ka: climatic,volcanic and tectonic controls

Sediments of Lake Van, Turkey, preserve one of the most complete records of continental climate change in the Near East since the Middle Pleistocene. We used seismic reflection profiles to infer past changes in lake level and discuss potential causes related to changes in climate, volcanism, and regional tectonics since the formation of the lake ca. 600 ka ago. Lake Van’s water level ranged by as much as 600 m during the past ~600 ka. Five major lowstands occurred, at ~600, ~365–340, ~290–230, ~150–130 and ~30–14 ka. During Stage A, between about 600 and 230 ka, lake level changed dramatically, by hundreds of meters, but phases of low and high stands were separated by long time intervals. Changes in the lake level were more frequent during the past ~230 ka, but less dramatic, on the order of a few tens of meters. We identified period B1 as a time of stepwise transgressions between ~230 and 150 ka, followed by a short regression between ca. 150 and 130 ka. Lake level rose stepwise during period B2, until ~30 ka. During the past ~30 ka, a regression and a final transgression occurred, each lasting about 15 ka. The major lowstand periods in Lake Van occurred during glacial periods, suggesting climatic control on water level changes (i.e. greatly reduced precipitation led to lower lake levels). Although climate forcing was the dominant cause for dramatic water level changes in Lake Van, volcanic and tectonic forcing factors may have contributed as well. For instance, the number of distinct tephra layers, some several meters thick, increases dramatically in the uppermost ~100 m of the sediment record (i.e. the past ~230 ka), an interval that coincides largely with low-magnitude lake level fluctuations. Tectonic activity, highlighted by extensional and/or compressional faults across the basin margins, probably also affected the lake level of Lake Van in the past.     

Climatic and anthropogenic influence on the stable isotope record from bulk carbonates and ostracodes in Lake Neuchâ, Switzerland, during the last two millennia

Lake Neuchåtel is a medium sized, hard-water lake, lacking varved sediments, situated in the western Swiss Lowlands at the foot of the Jura Mountains. Stable isotope data (18O and 13C) from both bulk carbonate and ostracode calcite in an 81 cm long, radiocarbon-dated sediment core represent the last 1500 years of Lake Neuchåtel's environmental history. Comparison between this isotopic and other palaeolimnologic data (mineralogical, geochemical, palynological, etc.) helps to differentiate between anthropogenic and natural factors most recently affecting the lake. An increase in lacustrine productivity (450–650AD ca), inferred from the positive trend in 13C values of bulk carbonate, is related to medieval forest clearances and the associated nutrient budget changes. A negative trend in both the bulk carbonate and ostracode calcite 18O values between approximately 1300 and 1500AD, is tentatively interpreted as due to a cooling in mean air temperature at the transition from the Medieval Warm Period to the Little Ice Age. Negative trends in bulk carbonate 18O and 13C values through the uppermost sediments, which have no equivalent in ostracode calcite isotopic values, are concomitant with the recent onset of eutrophication in the lake. Isotopic disequilibrium during calcite precipitation, probably due to kinetic factors in periods of high productivity is postulated as the mechanism to explain the associated negative isotopic trends, although the effect of a shift of the calcite precipitation towards the warmer months cannot be excluded.     

Climate and human impact on a meromictic lake during the last 6,000 years (Montcortès Lake,Central Pyrenees,Spain)

Sedimentological, mineralogical and compositional analyses performed on short gravity cores and long Kullenberg cores from meromictic Montcortès Lake (Pre-Pyrenean Range, NE Spain) reveal large depositional changes during the last 6,000 cal years. The limnological characteristics of this karstic lake, including its meromictic nature, relatively high surface area/depth ratio (surface area ~0.1 km²; z _max = 30 m), and steep margins, facilitated deposition and preservation of finely laminated facies, punctuated by clastic layers corresponding to turbidite events. The robust age model is based on 17 AMS ¹⁴C dates. Slope instability caused large gravitational deposits during the middle Holocene, prior to 6 ka BP, and in the late Holocene, prior to 1,600 and 1,000 cal yr BP). Relatively shallower lake conditions prevailed during the middle Holocene (6,000–3,500 cal years BP). Afterwards, deeper environments dominated, with deposition of varves containing preserved calcite laminae. Increased carbonate production and lower clastic input occurred during the Iberian-Roman Period, the Little Ice Age, and the twentieth century. Although modulated by climate variability, changes in sediment delivery to the lake reflect modifications of agricultural practices and population pressure in the watershed. Two episodes of higher clastic input to the lake have been identified: 1) 690–1460 AD, coinciding with an increase in farming activity in the area and the Medieval Climate Anomaly, and 2) 1770–1950 AD, including the last phase of the Little Ice Age and the maximum human occupation in late nineteenth and early twentieth centuries.     

Flood response to rainfall variability during the last 2000 years inferred from the Taravilla Lake record (Central Iberian Range, Spain)

A sedimentological, geochemical and palynological study of the Taravilla Lake sequence (Central Iberian Range, NE Spain) provides a detailed record of allochthonous terrigenous layers that intercalate within the lacustrine sediments over the last 2000 years. These terrigenous layers are interpreted as the result of extreme hydrological events that caused higher clastic input to the basin. Anthropogenic influence caused by fires or deforestation is rejected as the main factor generating these layers because human impact, inferred from the pollen reconstruction, was minimal when the terrigenous layers reached their greatest frequency. The reconstructed occurrence of these events in the Taravilla Lake record is coherent with the paleoflood history of the Tagus River, characterized by a notable increase of extreme events at the beginning of the Little Ice Age. The Taravilla record suggests a relationship between the occurrence of extreme hydrological events, solar variability, and the North Atlantic Oscillation for the NE Iberian Peninsula.     

Lake Jezero v Ledvici (NW Solvenia) – changes in sediment records over the last two centuries

Lake Jezero v Ledvici (NW Slovenia) is a 14 m deep mountain lake at an elevation of 1860 m, situated on limestone bedrock. It is an oligotrophic, alkaline and hard-water lake with a transparency of about 14 m and has suffered several times over recent centuries from strong earthquakes. In 1996 five sediment cores, between 35 and 45 cm long, were collected from the deepest part of the lake and analysed to reconstruct environmental changes over the last few centuries. The data indicate changes induced by pollution and climate change during the last two centuries similar to those in other European mountain and remote lakes. However, at this site earthquakes have also affected the lake and partly obscure the interpretation of the sediment record. From 1780 to 1890, sediment records show low abundance of diatoms and relatively high abundance of Cladocera. After 1890, the number of diatoms started to increase coinciding with a strong earthquake. From the beginning of the 20th century, concentrations of spheroidal carbonaceous particles (SCP), diatom valves and head capsules of chironomids gradually increased whilst in Cladocera the main difference observed was a change in the proportion of benthic taxa. After 1960, in parallel with a rise in air temperature, a further increase in accumulation rate of diatoms started, but there was a decrease in Cladocera.     

Variations in the oxygen-isotope composition of ancient Lake Superior between 10,600 and 8,800 cal BP

Variations in the oxygen-isotope composition of paleo-water bodies in the Lake Superior Basin provide information about the timing and pathways of glacial meltwater inflow into and within the Lake Superior Basin. Here, the oxygen-isotope compositions of Lake Superior have been determined using ostracodes from four sediment cores from across the Basin (Duluth, Caribou and Ile Parisienne sub-basins, Thunder Bay trough). The δ¹⁸O values indicate that lake water (Lake Minong) at ~10,600–10,400 cal [~9,400–9,250] BP was dominated by glacial meltwater derived from Lake Agassiz and the Laurentide Ice Sheet (LIS). From that time to ~9,000 cal [~8,100] BP, a period associated with formation of thick varves across the Lake Superior Basin, the δ¹⁸O values of Lake Minong decreased even further (−24 to −28‰), symptomatic of an increasing influx of glacial meltwater. Its supply was reduced between ~9,000 and ~8,900 cal [~8,100–8,000] BP, and lake water δ¹⁸O values grew higher by several per mil during this period. Between ~8,900 and ~8,800 cal [~8,000–7,950] BP, there was a return to δ¹⁸O values as low as −29‰ in some parts of the Lake Superior Basin, indicating a renewed influx of glacial meltwater before its final termination at ~8,800–8,700 cal [~7,950–7,900] BP. The sub-basins in the Lake Superior Basin generally displayed very similar patterns of lake water δ¹⁸O values, typical of a well-mixed system. The final stage of glacial meltwater input, however, was largely expressed near its input (Thunder Bay trough) and recognizable in dampened form mainly in the Duluth sub-basin to the west. Water in the easternmost Ile Parisienne sub-basin was enriched in ¹⁸O relative to the rest of the lake, particularly after ~10,000 cal [~8,900] BP, probably because of a strong influence of local precipitation/runoff, and perhaps also enhanced evaporation. By ~9,200 cal [~8,250] BP, lake water δ¹⁸O values in the Ile Parisienne sub-basin were similar to the adjacent Lake Huron Basin, suggesting a strong hydraulic connection between the two water bodies, and common responses to southern Ontario’s shift to warmer and dry climatic conditions after ~9,000 cal [~8,100] BP.     

Anthropogenic changes in the sediment composition of Lake Gości& aogon;ż (central Poland), during the last 330 yrs*

According to historical sources, the development of settlement around Lake Goci& aogon; during the last 330 yrs was intense at two time periods: the second half of the 1700's, and from ca. 1880 until 1944. The small farms were then abandoned, following which the lake surroundings were planted with forest trees. The presented study of human influence on the lake ecosystem and surrounding vegetation has been based on analyses of general sediment composition and its chemistry, pollen, Cyanobacteria, Chlorophyceae, Rotatoria, Cladocera, and a preliminary diatom survey. The history of human impact has been divided into four phases: 1. Phase of small local hamlets (before ca. 1770): The human impact was rather moderate then, but the cultivation of Canabis sativa, Secale cereale and later of Fagopyrum is evidenced from that time. 2. Phase of Hollandii settlement (ca. 1770-1863). Its influence is indicated first by the recession of deciduous wood (Corylus, Carpinus) stands, which triggered drastic drop of calcium in sediments. The development of rural economy in the area, including, an extension of agriculture (Secale and other cereals, crucifers, potatoes), and animal breeding based partly on grazing in the forest, is evidenced only after 1820. 3. Phase of German colonization (1863-1944): In the early periods (before 1910) the pollen spectra do not document any essential change in the type of farming, however, a serious disturbance of the lake ecosystem and sediment chemistry is evidenced by the blooms of Araphidinae diatoms and Tetraedron minimum, a maximum frequency of Bosmina longirostris, disturbances of the regular spring blooms of Chrysophyceae, appearance of vivianite, distinct maxima of organic matter, potassium, and iron concentration in sediments and an increase of the sedimentation rate. An intensification of agricultural activities commenced around 1910; woods, including also pinewoods, were then heavily devastated, and farming extended on poor soils, what was symptomatic for the general poverty of population. Coincidently in the lake, Centriceae showed blooms, Araphidinae diatoms and Tetraedron minimum developed, and the content of potassium, iron and phosphorus increased substantially, indicating altogether rising eutrophication. 4. Phase of restoration of the natural landscape (after 1945): The farm degradation from ca. 1944 is very weakly expressed in pollen data, which show a substantial fall of farming indicators from 1953/6 only, when the whole area was used for forest plantation. It was accompanied by a certainly spontaneous development of Betula and Alnus woods, this process progressing till recent time. The gradual extinction of farming activity near Lake Goci was accompanied by abrupt changes in the lake ecosystem, expressed by the restored blooms of Chrysophyceae expansion of planktonic Cladocera, rapid decline of phosphorus and extinction of vivianite from sediments. As documented by the drop of Cu/Zn ratio, lake hypolimnion has been weakly oxidized since 1949, what was probably responsible for the drop of Fe and Mn content in sediments. Increasing strength of overturns affected preservation of laminae in sediments, which almost completely disappeared after 1966.     

Late Quaternary (30.7–9.0 cal ka BP) vegetation history in Central Asia inferred from pollen records of Lake Balikun, northwest China

Surface and fossil pollen samples were collected to reconstruct the vegetation and environment since 30.7 cal ka BP in the Lake Balikun Basin, northwest China. This record demonstrates that the region was occupied by four vegetation communities during the interval 30.7–9.0 cal ka BP, including desert steppe (30.7–25.1 cal ka BP), Amaranthaceae-Asteraceae desert (25.1–17.7 cal ka BP), Amaranthaceae-Artemisia-Asteraceae desert (17.7–12.8 cal ka BP) and Amaranthaceae desert (12.8–9.0 cal ka BP), corresponding to relatively humid, the coldest and driest, cold and dry, and colder and drier conditions, respectively. The reconstructions of vegetation and environmental changes around Lake Balikun do not support the interpretation of an extremely humid LGM in Central Asia, challenging the cold-moist (glacial), warm-dry (interglacial) pattern of late Quaternary environmental change in Central Asia. The Last Glacial Maximum was cold and dry, and similarly harsh conditions continued into the early Holocene.     

Depositional rates and dating techniques of modern deposits in the Brno reservoir (Czech Republic) during the last 70 years

Facies analysis, magnetic susceptibility, and analysis of grain size, TOC content and isotopes (¹³⁷Cs, ²¹⁰Pb, ²²⁶Ra, ²²⁸Ra, and ²³⁸U concentrations) were used to determine the history of the modern deposits of the Brno reservoir. The sedimentary succession can be subdivided into two main units. The lower unit is formed predominantly by medium- to coarse-grained silty sands and is interpreted as a fluvial succession deposited before the Svratka River was dammed. The upper unit consists of brownish planar laminated silts and rarely of clayey or sandy silts and is interpreted as a product of the reservoir deposition. The concentrations of ²³⁸U reflect the history of uranium mining in the upper part of the Svratka River catchment. As a consequence, ²¹⁰Pb radionuclide concentrations cannot be used for establishing a sediment chronology. Concentrations of ¹³⁷Cs show two marked peaks, the upper of which is attributed to the Chernobyl reactor accident in 1986, and the lower one is attributed to the maximum rate of atomic weapons testing in 1963. From these peaks, mean depositional rates of 3.2 cm year⁻¹ for the time period of 1986–2007 and of 3.4 cm year⁻¹ between 1963 and 1986 are calculated. Based on the known age of the reservoir, which was constructed in 1939, we can also calculate mean depositional rate for the time period of 1939–1963, which is 3.1 cm year⁻¹.     

Benthic foraminifera and their stable isotope composition in sediment cores from Lake Qarun,Egypt: changes in water salinity during the past ~500 years

We studied the sedimentology, benthic foraminifera, molluscs, and δ¹⁸O and δ¹³C of Ammonia tepida tests in two late Holocene sediment cores from Lake Qarun (Egypt). The cores, QARU2 (upper section, 8.2 m) and QARU4 (1.4 m), span approximately the past 500 years of sedimentation. Benthic foraminifera first appeared in the upper part of QARU2 at 314 cm depth, ca. AD 1550. This depth marks the beginning of colonization of the lake by foraminifera and indicates a change in lake water salinity, as foraminifera cannot tolerate fresh water. Initially, three species of benthic foraminifera colonized the lake, Ammonia tepida, Cribroelphidium excavatum and Cribrononion incertum. Relative abundance of these species fluctuated throughout cores QARU2 and QARU4 and highest overall faunal diversity occurred at the beginning of the twentieth century. High relative abundances of C. incertum and deformed tests are attributed to periods of greater lakewater salinity. Peaks in both δ¹⁸O and δ¹³C indicate times of higher evaporation and reduced fresh water inflow. Inferred salinity was high around AD 1700 and after AD 1990. Rapid response of climate proxy variables indicates the high sensitivity of Lake Qarun to environmental changes over the past several 100 years. Increases in lakewater Mg concentration during past evaporative events, associated with less fresh water inflow, probably provided conditions suitable for C. incertum to build its white or transparent tests. Gradual decrease of C. incertum, until its disappearance at 100 cm depth ca. AD 1890, indicates a more persistent trend in lake water chemistry. Higher concentrations of dissolved sulphates were the likely cause of this species disappearance. Recent, twentieth-century sediments were deposited under optimal salinity (37‰) for benthic fauna, but further environmental changes are indicated by the decrease or disappearance of several benthic foraminifera and mollusc species. Intermittent hypoxia in the lake’s bottom waters, caused by cultural eutrophication, may account for these most recent changes.     

Isotopic fingerprints on lacustrine organic matter from Laguna Potrok Aike (southern Patagonia, Argentina) reflect environmental changes during the last 16,000 years

A combination of carbon-to-nitrogen ratios (TOC/TN), Rock Eval-analyses, and stable isotope values of bulk nitrogen (δ¹⁵N) and organic carbon (δ¹³C_org) was used to characterize bulk organic matter (OM) of a piston core from the Patagonian maar lake Laguna Potrok Aike (Argentina) for the purpose of palaeoenvironmental reconstruction. Sedimentary data were compared with geochemical signatures of potential OM sources from Laguna Potrok Aike and its catchment area to identify the sources of sedimentary OM. Correlation patterns between isotopic data and TOC/TN ratios allowed differentiation of five distinct phases with different OM composition. Before 8470 calibrated ¹⁴C years before present (cal. yrs BP) and after 7400 cal. yrs BP, isotopic and organo-geochemical fingerprints indicate that the sediments of Laguna Potrok Aike consist predominantly of soil and diatom OM with varying admixtures of cyanobacterial and aquatic macrophyte OM. For a short phase of the early Holocene (ca. 8470–7400 cal. yrs BP), however, extremely high input of soil OM is implied by isotopic fingerprints. Previous seismic and geochronological results indicate a severe lake-level drop of 33 m below present-day shortly before 6590 cal. yrs BP. It is suggested that this lake level drop was accompanied by increased erosion of shore banks and channel incision enhancing soil OM deposition in the lake basin. Thus, isotopic data can be linked to hydrological variations at Laguna Potrok Aike and allow a more precise dating of this extremely low lake level. An isotopic mixing model was used including four different sources (soil, cyanobacteria, diatom and aquatic macrophyte OM) to model OM variations and the model results were compared with quantitative microfossil data.     

Climate changes during the last glacial termination inferred from diatom-based temperatures and pollen in a sediment core from Längsee (Austria)

A sediment core section from Längsee, a small meromictic lake in the southern Alpine lowland (Carinthia, Austria) close to the Würmian ice margin, was investigated by means of diatoms and pollen. The main aims of the study were to reconstruct water temperature as a signal of climate change during the last glacial termination, compare the aquatic and terrestrial response to the changing climate, and place our findings into a climatic frame on the northern hemispheric scale. A calibration data set (ALPS06) of 116 lakes was constructed using data from newly studied lakes and from two previously published data sets and we established a transfer function for predicting summer epilimnetic water temperatures (SEWT). A locally weighted weighted average regression and calibration model (R _jack ²  = 0.89; RSMEP = 1.82°C) was applied to the fossil diatom assemblages in order to reconstruct SEWT. Three major sections were distinguished in the time window of approximately 19–13 cal ka BP, which fitted well with the oxygen isotope curve and the isotope-event stratigraphy from the Greenland ice-core GRIP. The first section was a warming period (SEWT range from 11.6 to 18.0°C; average 15.8°C = ca. 6°C below present) called the Längsee oscillation, which probably correlates with the warmer sub-section (GS-2b) of the Greenland Stadial 2. The subsequent section represents a climate cooling, called the Längsee cold period (SEWT range between 10.6 and 15.9°C; average 12.9°C), which probably corresponds with the sub-section GS-2a of the Greenland Stadial 2, the Heinrich 1 cold event of the North Atlantic, and partially the Gschnitz Stadial in the Alps. The Längsee cold period shows a tri-partition: Two colder phases are separated by a warmer inter-phase. The passive ordination of the core sample scores along maximum water depth indicated that the Längsee cold period was drier than the Längsee oscillation. Strong short-term fluctuations during the Längsee oscillation and the Längsee cold period indicate climate instability. The third section represented climate warming during the Längsee late glacial interstadial (=Greenland Interstadial 1, GI-1) with an average SEWT of 17.5°C. From the minor climatic fluctuations during this interstadial, mainly indicated by pollen, the fluctuation most likely related to the Gerzensee oscillation showed a SEWT decline. During the early immigration and expansion period of shrubs and trees, aquatic and terrestrial records showed distinct discrepancies that might have arose because of time lags in response and differences in sensitivity.     

Trace metal and geochemical variability during 5,500 years in the sediment of Lake Lehmilampi,Finland

A high-resolution geochemical profile from a 5,500-year-old sediment core of Lake Lehmilampi in eastern Finland was analyzed to study long-term trends and variability in element concentrations and accumulation rates. The accumulation rates of all studied elements followed the same trend, responding to changes in the total sedimentation rate. Concentration profiles differed among elements and showed considerable variation over time. Principal components analysis (PCA) was used on the concentration data to identify groups of elements that have similar geochemical controls. The first principal component was influenced by changes in mineral matter accumulation, and it incorporated elements that are associated with stable allochthonous minerals (such as Mg, K, Cs, Rb, Li, Ti and Ga), as well as elements in forms that become diluted when mineral matter increases (e.g., S, Fe and Mn). The second and third principal components showed that a large proportion of the variance was accounted for by elements with continuously increasing or decreasing concentrations related to pedogenetical development of the catchment soil. In the case of Hg, Pb and Cd, however, accumulation rates increased faster at the surface than is simply accounted for by changes in total sedimentation rates. For Cu, Cr, Ni and Zn, concentrations increased over the past 150 years, but there were no indications of a significant addition due to atmospheric deposition. These elements had more variable concentrations before the mid nineteenth century than after, as did elements that are often used for normalization. These findings suggest that lake sediments may not properly reflect the history of atmospheric metal deposition in remote areas.     

A diatom-inferred record of reduced effective precipitation during the Last Glacial Coldest Phase (28.8–18.0?cal kyr BP) and increasing Holocene seasonality at Lake Pupuke, Auckland, New Zealand

A continuous, 1,420-cm sediment record from Lake Pupuke, Auckland, New Zealand (37°S) was analysed for diatom taxonomy, concentration and flux. A New Zealand freshwater diatom transfer function was applied to infer past pH, electrical conductivity, dissolved reactive phosphorus and chlorophyll a. A precise, mixed-effect regression model of age versus depth was constructed from 11 tephra and 13 radiocarbon dates, with a basal age of 48.2?cal kyr BP. Diatom-inferred changes in paleolimnology and climate corroborate earlier inferences from geochemical analyses (Stephens et al. 2012), with respect to the timing of marked climate changes in the Last Glacial Coldest Phase (LGCP; 28.8?C18.0?cal kyr BP), the Last Glacial Interglacial Transition (LGIT; 18.0 to ca. 12?C10?cal kyr BP) and the Holocene, the onset of which is difficult to discern from LGIT amelioration, but which includes an early climatic optimum (10.2?C8.0?cal kyr BP). The LGCP is readily defined by a reduction in lake level and effective precipitation, whereas the LGIT represents a period of rising lake level, with greater biomass during the Holocene. There was limited change in diatom assemblage structure, influx or inferred water quality during a Late Glacial Reversal (LGR; 14.5?C13.8?cal kyr BP), associated with heightened erosional influx. In contrast, an LGIT peak in paleoproductivity is recorded by increased diatom influx from 13.8 to 12.8?cal kyr BP. Changes in sediment influx and biomass record complex millennial-scale events attuned to the Antarctic Cold Reversal (ACR; 14.5?C12.8?cal kyr BP). Additional millennial-scale environmental change is apparent in the Holocene, with marked changes in lake circulation beginning at 7.6?cal kyr BP, including the onset of seasonal thermal stratification and rapid species turnover at 5.7?cal kyr BP. The most rapid diatom community turnover accompanied widely varying nutrient availability and greater seasonality during the last 3.3?cal kyr. Rising seasonality appears to have been linked to strengthened Southern Westerlies at their northern margins during the middle and late Holocene.