Hypersalinity During Regional Drought Drives Mass Mortality of the Seagrass <Emphasis Type="Italic">Syringodium filiforme</Emphasis> in a Subtropical Lagoon

Seagrasses are sensitive to local environmental conditions such as salinity, the underwater light environment, and nutrient availability. To characterize seagrass coverage and condition, as well as to relate changes in community structure to local environmental and hydrologic conditions, we monitored seagrass communities in the Upper Laguna Madre (ULM), Texas annually from 2011 to 2015. In 2011 and 2012, the lagoon was dominated primarily by Halodule wrightii, with mixed meadows of H. wrightii and Syringodium filiforme located in the northwest of our study area. By 2013, the expansive S. filiforme meadows had disappeared and the species was restricted to the northernmost reaches of the lagoon. The S. filiforme mortality occurred following an extended period of extremely high salinity (salinities 50–70) during a regional drought. Continuous measurements of underwater photosynthetically active radiation and stable carbon isotopic signatures of seagrass blade tissues did not suggest light limitation, and H. wrightii N/P molar ratios near 30:1 were not indicative of nutrient limitation. Based on the absence of strong evidence for light or nutrient limitation, along with the known tolerance of H. wrightii for higher salinities, we conclude that hypersalinity driven by regional drought was likely the major driver behind the observed S. filiforme mortality. With a substantial portion of the global seagrass distribution threatened by drought in the next 50 years, the increased frequency of hypersaline conditions is likely to exacerbate stress in seagrass systems already vulnerable to the effects of rising water temperatures, eutrophication, and sea level rise.     

CAD assessment of the posture and range of motion of <Emphasis Type=Italic>Kentrosaurus aethiopicus</Emphasis> H<Emphasis Type=SmallCaps>ennig</Emphasis> 1915

A computer aided design analysis using high-resolution laser scans of the bones of the stegosaur Kentrosaurus aethiopicus Hennig 1915 from the Late Jurassic Tendaguru Formation indicates that in the habitual walking pose the forelimbs were probably held erect, and that strong humeral flexion and abduction mainly occurred in a defensive stance. Rapid gaits with unsupported phases could not be used. The neck allowed sufficient lateral flexion to guarantee good sight in all directions including posteriorly. The tail covered an arch of roughly 180° and had sufficient range to be used as a weapon. Possibly, the animal could accomplish tail blows against specific targets in sight. Also, a tripodal pose is suggested to have been possible, roughly doubling the maximum vertical feeding height of Kentrosaurus.     

Estimates of source parameters of <Emphasis Type=Italic>M</Emphasis>4.9 Kharsali earthquake using waveform modelling

This paper presents the computation of time series of the 22 July 2007 M 4.9 Kharsali earthquake. It occurred close to the Main Central Thrust (MCT) where seismic gap exists. The main shock and 17 aftershocks were located by closely spaced eleven seismograph stations in a network that involved VSAT based real-time seismic monitoring. The largest aftershock of M 3.5 and other aftershocks occurred within a small volume of 4 × 4 km horizontal extent and between depths of 10 and 14 km. The values of seismic moment (M _∘) determined using P-wave spectra and Brune’s model based on f ² spectral shape ranges from 10¹⁸ to 10²³ dyne-cm. The initial aftershocks occurred at greater depth compared to the later aftershocks. The time series of ground motion have been computed for recording sites using geometric ray theory and Green’s function approach. The method for computing time series consists in integrating the far-field contributions of Green’s function for a number of distributed point source. The generated waveforms have been compared with the observed ones. It has been inferred that the Kharsali earthquake occurred due to a northerly dipping low angle thrust fault at a depth of 14 km taking strike N279°E, dip 14° and rake 117°. There are two regions on the fault surface which have larger slip amplitudes (asperities) and the rupture which has been considered as circular in nature initiated from the asperity at a greater depth shifting gradually upwards. The two asperities cover only 10% of the total area of the causative fault plane. However, detailed seismic imaging of these two asperities can be corroborated with structural heterogeneities associated with causative fault to understand how seismogenesis is influenced by strong or weak structural barriers in the region.     

Bacterial Community Dynamics in a Seagrass (<Emphasis Type="Italic">Posidonia oceanica</Emphasis>) Meadow Sediment

Some traits of the bacterial community dynamics associated to the rhizosphere of the Mediterranean seagrass Posidonia oceanica, growing in carbonate sediments, were analyzed during a 2-year period in an enclosed bay of the Balearic Islands. The diversity of the bacterial community was studied by the construction of 16S rDNA clone library. For testing temporal and vertical differences in the abundance of total cells and active Bacteria, we used 4′,6-diamidino-2-phenylindole (DAPI) staining and fluorescence in situ hybridization (FISH). Moreover, some relevant groups of sulfate-reducing bacteria (SRB) were occasionally assessed by FISH. Despite the observed decrease in the total DAPI-stained cells, bacterial counts, and sulfate reduction rates throughout the sampling time, we found an increase in both the pore-water sulfide concentration and the proportion of SRB. Overall, the results revealed a very high bacterial diversity and indicated shifts in bacterial dynamics that could not be related to temperature-dependent factors, suggesting a link between the documented regression of the seagrass meadow and the decline of the microbial community, likely due to large organic matter inputs to the bay.     

Deterioration of Sediment Quality in Seagrass Meadows (<Emphasis Type="Italic">Posidonia oceanica</Emphasis>) Invaded by Macroalgae (<Emphasis Type="Italic">Caulerpa</Emphasis> sp.)

Species of the macroalgae Caulerpa sp. are increasingly being observed in meadows of the endemic Mediterranean seagrass Posidonia oceanica, and in particular Caulerpa taxifolia, has been considered as an invasive species leading to seagrass decline. Studies have so far failed to reveal the underlying mechanisms of the success of the macroalgae, and here, we examine how biogeochemical changes of the environment associated to indigenous (Caulerpa prolifera) and non-indigenous (Caulerpa racemosa and C. taxifolia) species affect the habitat of P. oceanica. Two of the species (C. prolifera and C. racemosa) affect the sediment biogeochemical conditions by increasing organic matter pools, microbial activity, and sulfide pools of the sediments, and limited effects were found for C. taxifolia. Biomass of the macroalgae contributed to the extent of impacts, and high sulfide invasion into the seagrasses and regression of the meadow were pronounced at the location with the highest Caulerpa biomass. This suggests that Caulerpa invasion contributes to seagrass decline probably because Caulerpa thrives better than the seagrasses in the modified environment.     

The Bloom-Forming Macroalgae, <Emphasis Type="Italic">Ulva</Emphasis>, Outcompetes the Seagrass, <Emphasis Type="Italic">Zostera marina</Emphasis>, Under High CO<Subscript>2</Subscript> Conditions

While multiple species of macroalgae and seagrass can benefit from elevated CO₂ concentrations, competition between such organisms may influence their ultimate responses. This study reports on experiments performed with a Northwest Atlantic species of the macroalgae, Ulva, and the seagrass, Zostera marina, grown under ambient and elevated levels of pCO₂, and subjected to competition with each other. When grown individually, elevated pCO₂ significantly increased growth rates and productivity of Ulva and Zostera, respectively, beyond control treatments (by threefold and 27%, respectively). For both primary producers, significant declines in tissue δ¹³C signatures suggested that increased growth and productivity were associated with a shift from use of HCO₃^? toward CO₂ use. When grown under higher pCO₂, Zostera experienced significant increases in leaf and rhizome carbon content as well as significant increases in leaf carbon-to-nitrogen ratios, while sediments within which high CO₂ Zostera were grown had a significantly higher organic carbon content. When grown in the presence of Ulva; however, above- and below-ground productivity and tissue nitrogen content of Zostera were significantly lower, revealing an antagonistic interaction between elevated CO₂ and the presence of Ulva. The presence of Zostera had no significant effect on the growth of Ulva. Collectively, this study demonstrates that while Ulva and Zostera can each individually benefit from elevated pCO₂ levels, the ability of Ulva to grow more rapidly and inhibit seagrass productivity under elevated pCO₂, coupled with accumulation of organic C in sediments, may offset the potential benefits for Zostera within high CO₂ environments.     

Seismic piezocone interpretation for shear wave velocity (<Emphasis Type=Italic>V</Emphasis><Subscript>S</Subscript>) determination in the Persian Gulf

Shear wave velocity is one of the important factors representing the dynamic characteristics of soil layers. Hence, many researchers have focused their studies on determining shear wave velocity by direct field measurements or expressions developed by other soil parameters. The shear module and damping ratio of the soil layers also play a similar role in the majority of dynamic soil response analyses. Nevertheless, since they have to be measured in the laboratory by resonant column or cyclic triaxial tests on undisturbed samples, the possibility of preparing such samples and the reliability of the obtained results are of great concerns. In the present study, great effort has been made to determine the above dynamic factors by means of field data obtained from a versatile instrument, namely the seismic piezocone (SPCT_U), and to derive expressions correlating them with some parameters obtainable by much simpler instruments. The reliability of laboratory measurements on undisturbed samples is also evaluated. The seismic piezocone test apparatus has been employed to evaluate the soil properties at 1-m depth intervals by means of measuring tip resistance, sleeve resistance, pore pressure and shear wave velocity. The shear module and the damping ratio are calculated using field data. Meanwhile, in order to assess the laboratory measurements of these parameters, some resonant column tests and cyclic triaxial tests on undisturbed samples of the same soil layers have been carried out. In order to compare the field results of shear modulus and damping ratios with those obtained from laboratory tests, the influences of the soil nature and sample disturbance on the conventional laboratory methods are evaluated and discussed. The shear wave velocity is correlated to overburden pressure and the corrected tip resistance for two groups of fine soils, namely silty clays and carbonate clayey silts, which mainly cover the areas under study in this project, are located in southern parts of Iran near the Persian Gulf. According to the results of the present study, there are narrow limits of shear modulus regarding soils for which the laboratory tests and the field measurements yield approximately the same shear modulus. This limit of shear modulus is about 30–50(MPa) for clay deposits and 70–100 (MPa) for sandy deposits. Also the shear wave velocity can be calculated by a simple expression from total overburden pressure and the tip resistance of simple cone penetration test results conventionally available in many soil explorations prior to engineering practices. However, if the pore pressure inside the saturated soil deposits can be measured by a piezocone apparatus, the shear wave velocity may be calculated using another suggested equation in terms of effective overburden pressure in the present study. Regarding the shear module and the damping ratio, due to the disturbances of the stiff deposits in the sampling process and great deviations of laboratory results from field results, the laboratory measurements of these parameters out of the above limits are not recommended.     

BaCO<Subscript>3</Subscript>: high-temperature crystal structures and the <Emphasis Type="Italic">Pmcn</Emphasis>→<Emphasis Type="Italic">R</Emphasis>3<Emphasis Type="Italic">m</Emphasis> phase transition at 811°C

The temperature (T) evolution of the barium carbonate (BaCO₃) structure was studied using Rietveld structure refinements based on synchrotron X-ray diffraction and a powdered synthetic sample. BaCO₃ transforms from an orthorhombic, Pmcn, α phase to a trigonal, R3m, β phase at 811°C. The orthorhombic BaCO₃ structure is isotypic with aragonite, CaCO₃. In trigonal R3m BaCO₃, the CO₃ group occupies one orientation and shows no rotational disorder. The average <Ba–O> distances increase while the <C–O> distances decrease linearly with T in the orthorhombic phase. After the 811°C phase transition, the <Ba–O> distances increase while C–O distances decrease. There is also a significant volume change of 2.8% at the phase transition.     

The relation of seasonal pattern in stable carbon compositions to meteorological variables in the leaves of <Emphasis Type="Italic">Sabina</Emphasis><Emphasis Type="Italic">przewalskii</Emphasis> Kom. and <Emphasis Type="Italic">Sabina chinensis</Emphasis> (Lin.) Ant.

The seasonal variation of foliar δ¹³C values in Sabina przewalskii Kom. and Sabina chinensis (Lin.) Ant. was measured. The relationships between foliar δ¹³C values and branch δ¹³C values as well as environmental factors (monthly total precipitation, monthly average air temperature, monthly average soil temperature, monthly total solar duration, relative humidity, atmospheric pressure, vapor pressure, wind speed and potential evaporation) were investigated. The results showed that the foliar δ¹³C values were negatively correlated with air pressure, and positively correlated with air temperature, precipitation, vapor pressure, potential evaporation, solar duration, wind speed and soil temperature. No significant relationship between δ¹³C values and relative humidity was detected. This demonstrates that the foliar δ¹³C of Sabina is a successful empirical indictor of these meteorological factors within the usual range of C₃ whole-leaf δ¹³C values. Furthermore, the δ¹³C signature of leaf tissue is similar to that of wood tissue and the responses of δ¹³C values in S. przewalskii Kom. to environmental factors are also relatively stronger than that of S. chinensis (Lin.) Ant. These results provided strong evidence that it is feasible to extract climatic information from tree-ring δ¹³C series and S. przewalskii Kom. is a dendroclimatologically promising tree species.     

Net Primary Productivity of <Emphasis Type=Italic>Spartina densiflora</Emphasis> Brong in an SW Atlantic Coastal Salt Marsh

Coastal marshes are known as organic matter producers. The goal of this work is to study tiller demography, standing biomass, and net aerial primary productivity (NAPP) in a Spartina densiflora coastal wetland, using a method applied to permanent sample plots located at two sites differing in topographic location, a regularly flooded zone [relative low marsh (LM)] and an irregularly flooded one [relative high marsh (HM)]. Measurements were made every 2 months during the 2005–2007 period. The annual NAPP was estimated to be 2,599?±?705 gDW m^?2?year^?1 for the HM and 2,181?±?605 gDW m^?2?year^?1 and 602?±?154 gDW m^?2?year^?1 for the first and second period of the LM populations, respectively, showing a seasonal pattern reaching maximum values in summer. The reduced NAPP values of the LM sites in the second year was associated with an extremely high precipitation period related to the 2007–2008 El Niño event.     

Long-term declines in two apex predators,bull sharks (<Emphasis Type="Italic">Carcharhinus leucas</Emphasis>) and alligator gar (<Emphasis Type="Italic">Atractosteus spatula</Emphasis>), in Lake Pontchartrain,an oligohaline estuary in southeastern Louisiana

We analyzed historic and current fishery independent data to determine if the abundance of two apex predators, bull sharks (Carcharhinus leucas) and alligator gar (Atractosteus spatula), in Lake Pontchartrain had changed significantly over the last half century. Lake Pontchartrain is an environmentally degraded oligohaline estuary in southeastern Louisiana that has experienced considerable changes in fish assemblage composition over this interval. Using gillnet, beach seine, and trawl data collected during three time periods (1953–1955, 1977–1978, and 1996–2005), we analyzed trends in abundance forC. leucas andA. spatula using generalized linear models with a negative binomial error structure and a log link. Lake Pontchartrain data were divided into four spatial locations (northwest, northeast, southwest, southeast) since each region represents a unique combination of anthropogenic and natural influences that could affect catches. For each species and gear type, we produced log-likelihood profiles for the instantaneous rate of change in relative abundance through time. Raw catches were generally lower for both species in the later surveys.C. leucas were not captured in beach seines since the 1950s andA. spatula were rarely captured in trawls or seines since the 1970s. Likelihood profiles of changes in abundance forC. leucas andA. spatula showed very large declines in both species since 1953.C. leucas declined by 98.6% (95% CI: 73.4–99.9%) in gillnets and became functionally extirpated in beach seines with a decline of 99.9% (95% CI: 23–99.9%). Among all gears,C. leucas declined by the same rate as in gillnets. The decline inA. spatula was also large with a decrease of 98.6% (95% CI: 73.4–99.9%) in beach seines and a decline of 99.2% (95% CI: 54.8–99.9%) in trawls since 1953. Catches ofA. spatula in gillnets did not show a significant change over the study period. The continued decline of these two apex predators could seriously affect efforts to restore this degraded estuarine ecosystem.     

Performance of elevated tanks in<Emphasis Type="Italic">M</Emphasis><Subscript><Emphasis Type="Italic">w</Emphasis></Subscript> 7.7 Bhuj earthquake of january 26th, 2001

The current designs of supporting structures of elevated water tanks are extremely vulnerable under lateral forces due to an earthquake and the Bhuj earthquake provided another illustration when a great many water tank stagings suffered damage and a few collapsed. The more popular shaft type stagings suffer from poor ductility of thin shell sections besides low redundancy and toughness whereas framed stagings consist of weak members and poor brace-column joints. A strength analysis of a few damaged shaft type stagings clearly shows that all of them either met or exceeded the strength requirements of IS:1893-1984, however, they were all found deficient when compared with requirements of the International Building Code. IS:1893-1984 is unjustifiably low for these systems which do not have the advantage of ductility and redundancy and are currently being underestimated at least by a factor of 3 and need an upward revision of forces immediately.     

Responses of <Emphasis Type=Italic>Avicennia germinans</Emphasis> (Black Mangrove) and the Soil Microbial Community to Nitrogen Addition in a Hypersaline Wetland

The responses of dwarf black mangroves (Avicennia germinans) and components of the microbial community to the addition of nitrate over a 2-year period were examined. The field study was conducted in the Indian River Lagoon (Florida) in a mangrove-dominated impoundment that was established for purposes of mosquito control. The responses of mangroves to the regular addition of nitrate were insignificant or relatively minor compared to responses of the components of the microbial community. Denitrification rates, measured in the field and laboratory, increased significantly in fertilized plots and nitrous oxide emission rates were almost six times higher in fertilized plots. Nitrogen fixation was significantly lower in fertilized plots. Results suggest that mangrove systems in the N-limited Indian River Lagoon are likely to be long-term sinks for any increases in N loading.     

Food and habitat partitioning in grazing snails (<Emphasis Type="Italic">Turbo smaragdus</Emphasis>), Northern New Zealand

Gut content analyses and fatty acid profiles were used to identity food consumption and assimilation in a dominant grazing snail (Turbo smaragdus) in rocky shore and estuarine habitats, at Waiwera Estuary, northern New Zealand. Gut contents of freshly collected individuals indicated that snails utilize a wide range of food sources within their habitats, including microalgae and foliose-corticated macrophytes (rocky intertidal), and mangrove tissue and filamentous algae (mangrove stand). Laboratory feeding experiments revealed thatT. smaragdus prefers microalgae and possibly filamentous epiphytes, regardless of snail size or habitat of origination. The fatty acid profiles of snails fed different diets (brown algae [Hormosira banksii], diatoms, mangrove pneumatophores, and filamentous green algae [Chaetomorpha sp.]) confirmed the assimilation of the given foods, except for the mangrove treatment, which resulted in a low, long chain, fatty acid (LCFA) signature. Incongruities between the gut content analyses (high number of mangrove particles) and fatty acid profiles (low LCFA signature) of field and laboratory snails exposed to a mangrove diet suggest that snails target microalgal and filamentous epiphyte food sources on the pneumatophores and inadvertently ingest mangrove particles. Snails within all food treatments, except diatoms, had a relatively strong bacterial signature (18∶1n–7, Σ15+Σ17). Bacteria may have readily accumulated in the experimental tanks and been selected by snails as an alternative food source. In the field, snails and other grazers may ingest detritus and biofilms to access dense populations of bacteria. This research highlights the need for a comprehensive and multianalytical approach to elucidate the role of grazers in algal-plant dominated ecosystems.     

Seasonal movement and resource-use patterns of resident horseshoe crab (<Emphasis Type="Italic">Limulus polyphemus</Emphasis>) populations in a Maine,USA Estuary

Knowledge of resource-use and movement patterns is a missing component in the development of horseshoe crab (Limulus polyphemus) management strategies. Available evidence indicates the potential for a variety of possible migratory behaviors, but the lack of high-resolution, spatial-temporal data has hindered development of a year-round profile of ranging behavior. This need was addressed in the present study by using acoustic telemetry to track the movements of adult horseshoe crabs in two subembayments (Egypt and Hog Bays) of the Taunton Bay Estuary, Maine, from June 2003 to June 2005. Estimated mean total home range sizes were 64.1 and 61.4 ha for breeding crabs tagged in Egypt and Hog Bays, respectively. We observed no horseshoe crab dispersal to areas outside of the subembayments where they were tagged, so no mixing was observed between Egypt and Hog Bay individuals despite a < 4-km separation. Observed shifts in movement patterns, resource use (subtidal versus intertidal), and vagility facilitated a profile of seasonally partitioned horseshoe crab activity, which included late April to early May post-wintering, June–July breeding, August–September pre-wintering, and October–April wintering, where space usage represented about 10% of the mean total home range size. The apparent isolation of these resident populations implies a heightened vulnerability to overexploitation and large-scale habitat alteration that might be more easily sustained by larger, more vagile populations. This work underscores the need to apply horseshoe crab conservation, research, and management efforts at scales that are appropriate to the ranging patterns of crabs, which first requires application of high-resolution methods to identify those patterns.