Microphytobenthos contribution to nutrient-phytoplankton dynamics in a shallow coastal lagoon

Nutrient fluxes and primary production were examined in Lake Illawarra (New South Wales, Australia), a shallow (Z_mean=1.9 m) coastal lagoon with a surface area of 35 km², by intensive measurement of dissolved nutrients and oxygen profiles over a 22-h period. Rates of primary production and nutrient uptake were calculated for the microphytobenthos, seagrass beds, macroalgae, and pelagic phytoplankton. Although gross nutrient release rates to the water column and sediment pore waters were potentially high, primary production by microphytobenthos rapidly sequesters the re-mineralized nutrients so that net releases, averaged over times longer than a day, were low. Production in the water column was closely coupled with the relatively low sediment net nutrient release rates and detrital decomposition in the water column. Dissolved inorganic nitrogen and silica concentrations in the water column are drawn down at the beginning of the day. The system did not appear to be light limited so photosynthesis occurs as fast as the nutrients become available to the phytoplankton and microphytobenthos. We conjecture that microphytobenthos are the dominant primary producers and, as has been shown previously, that the nutrient uptake occurs in phase with the various stages of the diatom growth.     

Measured and modeled effects of benthic algae on eutrophication in Indian River-Rehoboth Bay, Delaware

A combination of field studies and mathematical modeling was used to examine the role of subtidal benthic algae in the eutrophication processes in two shallow estuarine systems. Field measurements indicated uptake by benthic algae retained ammonium and phosphate in the sediments when light at the sediment surface exceeded ≈150 μE m² s^?1. The measurements were used to calibrate a newly developed model of benthic algal activity. The benthic algal model was coupled with a hydrodynamic model, a eutrophication model, and a sediment diagenesis model. In the simulated ecosystem, benthic algae had a major influence in the intra-annual cycling of nitrogen and phosphorus. When nutrients were abundant in the water column (late winter and spring) they were transferred to the sediments through algal activity. Diagenesis released these nutrients to the water column in summer when nutrients were scarce. As a result of the nutrient transfer, annual primary production in the water column, in the presence of benthic algae, exceeded production in the absence of the algae.     

Microphytobenthos: The ecological role of the “secret garden” of unvegetated,shallow-water marine habitats. I. Distribution,abundance and primary production

The microphytobenthos consists of unicellular eukaryotic algae and cyanobacteria that grow within the upper several millimeters of illuminated sediments, typically appearing only as a subtle brownish or greenish shading. The surficial layer of the sediment is a zone of intense microbial and geochemical activity and of considerable physical reworking. In many shallow ecosystems, the biomass of benthic microalgae often exceeds that of the phytoplankton in the overlying waters. Direct comparison of the abundance of benthic and suspended microalgae is complicated by the means used to measure biomass and by the vertical and horizontal distribution of the microphytobenthos in the sediment. Where biomass has been estimated as chlorophyll a, there may be negligible to large (40%) error due to interference by degradation products, except where chlorophyll is measured by high-performance liquid chromatography. The vertical distribution of microphytobenthos, aside from mat-forming species, is determined by the opposing effects of their vertical migration, which tends to concentrate them near the surface, and physical mixing by overlying currents, which tends to cause an even vertical distribution through the mixed layer of sediment. Uncertainties in vertical distribution are compounded by frequently patchy horizontal distribution. Under-sampling on small (<1 m) scales can lead to errors in the estimate that are comparable to the ranges of seasonal and geographic variation. These uncertainties are compounded by biases in the techniques used to estimate production by the microphytobenthos. In most environments studied, biomass (as chlorophyll a) and light availability appear to be the principal determinants of benthic primary production. The effect of variable light intensities on integral production can be described by a functional response curve. When normalized to the chlorophyll content of the surficial sediment, the residual variation in the data described by the functional response curve is due to changes in the chlorophyll-specific response to irradiance. Production by the benthos is often a significant fraction of production in the water column and microphytobenthos may contribute directly to water column production when they are resuspended. Thus on both the basis of biomass and biogeochemical reactivity, benthic microalgae play significant roles in system productivity and trophic dynamics, as well as such habitat characteristics as sediment stability. *** DIRECT SUPPORT *** A01BY074 00003     

Drivers of Change in Shallow Coastal Photic Systems: An Introduction to a Special Issue

Coastal ecosystems are characterized by relatively deep, plankton-based estuaries and much shallower systems where light reaches the bottom. These latter systems, including lagoons, bar-built estuaries, the fringing regions of deeper systems, and other systems of only a few meters deep, are characterized by a variety of benthic primary producers that augment and, in many cases, dominate the production supplied by phytoplankton. These “shallow coastal photic systems” are subject to a wide variety of both natural and anthropogenic drivers and possess numerous natural “filters” that modulate their response to these drivers; in many cases, the responses are much different from those in deeper estuaries. Natural drivers include meteorological forcing, freshwater inflow, episodic events such as storms, wet/dry periods, and background loading of optically active constituents. Anthropogenic drivers include accelerated inputs of nutrients and sediments, chemical contaminants, physical alteration and hydrodynamic manipulation, climate change, the presence of intensive aquaculture, fishery harvests, and introduction of exotic species. The response of these systems is modulated by a number of factors, notably bathymetry, physical flushing, fetch, sediment type, background light attenuation, and the presence of benthic autotrophs, suspension feeding bivalves, and fringing tidal wetlands. Finally, responses to stressors in these systems, particularly anthropogenic nutrient enrichment, consist of blooms of phytoplankton, macroalgae, and epiphytic algae, including harmful algal blooms, subsequent declines in submerged aquatic vegetation and loss of critical habitat, development of hypoxia/anoxia particularly on short time scales (i.e., “diel-cycling”), fish kills, and loss of secondary production. This special issue of Estuaries and Coasts serves to integrate current understanding of the structure and function of shallow coastal photic systems, illustrate the many drivers that cause change in these systems, and synthesize their varied responses.     

Wind-driven sediment suspension controls light availability in a shallow coastal lagoon

Light availability is critically important for primary productivity in coastal systems, yet current research approaches may not be adequate in shallow coastal lagoons. Light attenuation in these systems is typically dominated by suspended sediment, while light attenuation in deeper estuaries is often dominated by phytoplankton. This difference in controls on light attenuation suggests that physical processes may exert a greater influence on light availability in coastal lagoons than in deeper estuaries. Light availability in Hog Island Bay, a shallow coastal lagoon on the eastern shore of Virginia, was determined for a summer and late fall time period with different wind conditions. We combined field measurements and a process-based modeling approach that predicts sediment suspension and light availability from waves and currents to examine both the variability and drivers of light attenuation. Total suspended solids was the only significant predictor of light attenuation in Hog Island Bay. Waves and currents in Hog Island Bay responded strongly to wind forcing, with bottom stresses from wind driven waves dominant for 60% of the modeled area for the late fall period and 24% of the modeled area for the summer period. Higher wind speeds in late fall than in summer caused greater sediment suspension (41 and 3 mg l⁻¹ average, respectively) and lower average (spatial and temporal) downwelling light availability (32% and 55%, respectively). Because of the episodic nature of wind events and the spatially variable nature of sediment suspension, conventional methods of examining light availability, such as fair-weather monitoring or single in situ recorders, do not adequately represent light conditions for benthic plants.     

Phytoplankton Spatial Variability in the River-Dominated Estuary,Apalachicola Bay,Florida

In shallow estuaries with strong river influence, the short residence time and pronounced gradients generate an environment for plankton that differs substantially in its dynamics from that of the open ocean, and the question arises “How is phytoplankton biomass affected?” This study assesses the small-scale spatial and temporal distribution of phytoplankton in Apalachicola Bay, a shallow bar-built estuary in the Florida Panhandle. Phytoplankton peaks were characterized to gain insights into the processes affecting spatial heterogeneity in biomass. Chlorophyll a (Chl a) distribution at 50-m spatial resolution was mapped using a flow-through sensor array, Dataflow©, operated from a boat that sampled four transects across the bay every 2 weeks for 16 months. Chl a peaks exceeding background concentrations had an average width of 1.3?±?0.7 km delineated by an average gradient of 3.0?±?6.0 μg Chl a L^?1 km^?1. Magnitude of E-W wind, velocity of N-S wind, tidal stage, and temperature affected peak characteristics. Phytoplankton contained in the peaks contributed 7.7?±?2.7% of the total integrated biomass observed along the transects during the study period. The river plume front was frequently a location of elevated Chl a, which shifted in response to river discharge. The results demonstrate that despite the shallow water column, river flushing, and strong wind and tidal mixing, distinct patchiness develops that should be taken into consideration in ecological studies and when assessing productivity of such ecosystems.     

Suspended-Sediment Flux and Retention in a Backwater Tidal Slough Complex near the Landward Boundary of an Estuary

Backwater tidal sloughs are commonly found at the landward boundary of estuaries. The Cache Slough complex is a backwater tidal region within the Upper Sacramento–San Joaquin Delta that includes two features that are relevant for resource managers: (1) relatively high abundance of the endangered fish, delta smelt (Hypomesus transpacificus), which prefers turbid water and (2) a recently flooded shallow island, Liberty Island, that is a prototype for habitat restoration. We characterized the turbidity around Liberty Island by measuring suspended-sediment flux at four locations from July 2008 through December 2010. An estuarine turbidity maximum in the backwater Cache Slough complex is created by tidal asymmetry, a limited tidal excursion, and wind-wave resuspension. During the study, there was a net export of sediment, though sediment accumulates within the region from landward tidal transport during the dry season. Sediment is continually resuspended by both wind waves and flood tide currents. The suspended-sediment mass oscillates within the region until winter freshwater flow pulses flush it seaward. The hydrodynamic characteristics within the backwater region such as low freshwater flow during the dry season, flood tide dominance, and a limited tidal excursion favor sediment retention.     

Intra-annual Variability in Primary Producer Groups and Nitrogen Dynamics in an Intermittently Closed Estuary Exposed to Mediterranean Climate

Nutrient dynamics in estuaries are temporally variable in response to changing physical–chemical conditions and biogeochemical processes involving primary producer groups such as phytoplankton, microphytobenthos, seagrass and macroalgae. In order to reveal intra-annual changes in the biomass of primary producer groups and associated changes in estuarine nutrient dynamics, we developed a box model, coupling water inflows and outflows and nitrogen dynamics in Wilson Inlet, a large, central-basin-dominated, intermittently closed estuary exposed to a Mediterranean climate in Western Australia. The model is calibrated and validated with monitoring data, aquatic plant biomass estimates and biogeochemical rate measurements. Macrophytes and their microalgal epiphytes appear to rapidly assimilate nutrients from the first flush from the catchment in winter, but this buffer capacity then ceases, and a phytoplankton ‘bloom’ develops in response to subsequent river runoff events in spring. Significant amounts of bioavailable nitrogen are exported to the ocean because phytoplankton predominance occurs while the sand bar is breached. Surface sediments play a key role for nitrogen dynamics: In late spring to autumn, high light availability at the sediment surface stimulates high primary production by microphytobenthos, leading to reduced benthic ammonium fluxes particularly in the deep basin. Microphytobenthos contributes about 60% of annual whole-system primary production. Despite high benthic primary production, nitrogen release from sediments is the biggest nitrogen source to the estuary.     

The relative importance of chlorophyll and colored dissolved organic matter (CDOM) to the prediction of the diffuse attenuation coefficient in shallow estuaries

The availability of underwater light is a critical factor in the growth and abundance of primary producers in shallow embayments. The goal of this study was to examine the relative importance of factors influencing light availability in this type of water body. Many simulation models of aquatic ecosystems predict light attenuation from chlorophyll or phytoplankton stock. In the three southern New England sites studied here, no useful relationship was found to exist between chlorophyll and K_PAR (the diffuse attenuation coefficient of photosynthetically active radiation; Kirk 1994; Mobley 1994). In 40 of 53 cases, a regression of chlorophyll versus K_PAR was not statistically significant. Variation in K_PAR did demonstrate a correlation to salinity, implicating a freshwater source of light attenuating material. This was true even in a system with little freshwater inflow. Colored dissolved organic matter (CDOM) is one such terrestrial input that enters estuaries from their watersheds and can strongly influence the availability of light to aquatic primary producers. This study demonstrated that over 70% of the variability in the K_PAR coefficient can be attributed to CDOM in the shallow estuaries studied. This illustrates the need for improved model formulations that include CDOM in the prediction of light attenuation in shallow coastal systems. A new equation has been developed to predict K_PAR with CDOM.     

Seasonal Alternation Between Groundwater Discharge and Benthic Coupling as Nutrient Sources in a Shallow Coastal Lagoon

Submarine groundwater discharge (SGD) is now recognized as an important source of nutrients and freshwater to some coastal environments. We studied a shallow coastal lagoon (Little Lagoon, AL, USA) in the northern Gulf of Mexico that lacks riverine inputs but has been suspected to receive significant SGD. We observed persistent salinity gradients between the east and west ends of the lagoon and the pass connecting it to the Gulf of Mexico. Covariance between salinity in the lagoon and the groundwater tracer ²²²Rn indicated that SGD was responsible for the salinity gradients and is the primary source of freshwater to the lagoon. Cluster analysis of 246 biweekly samples based on temperature, salinity, and two proxies of SGD revealed two hydrographic regimes with different drivers for nutrient inputs. In samples characterized by high discharge and low temperatures (generally December–April), total nitrogen (TN) was negatively correlated with salinity, while total phosphorus (TP) was positively correlated with temperature. Total nitrogen in the groundwater was very high (0.36–4.80 mM) while total phosphorus was relatively low (0.3–2.3 μM), consistent with SGD as the source of TN during the high-discharge periods. In periods with low discharge and higher temperatures (approx. May–November), TN and TP had strong positive correlations with temperature and are inferred to originate from benthic efflux. Seasonal changes in nutrient stoichiometry in the lagoon water column also indicate an alternation between low TN/TP sediments and high TN/TP groundwater as the primary sources of nitrogen in this system.     

Denitrification and N2O production in near-shore marine sediments

Methods were developed for determining rates of denitrification in coastal marine sediments by measuring the production of N₂ from undisturbed cores incubated in gas-tight chambers. Denitrification rates at summer temperatures (23°C) in sediment cores from Narragansett Bay, Rhode Island, were about 50μmol N₂m^?2 hr^?1. This nitrogen flux is equal to approximately one-half of the NH⁺₄flux from the sediments at this temperature and is of the magnitude necessary to account for the anomalously low N/P and anomalously high O/N ratios often reported for benthic nutrient fluxes. The loss of fixed nitrogen as N₂ during the benthic remineralization of organic matter, coupled with the importance of benthic remineralization processes in shallow coastal waters may help to explain why the availability of fixed nitrogen is a major factor limiting primary production in these areas. Narragansett Bay sediments are also a source of N₂O, but the amount of nitrogen involved was only about 0.2 μmol m^?2 hr^?1 at 23°C.     

Oyster (<Emphasis Type="Italic">Crassostrea virginica</Emphasis>) Aquaculture Shifts Sediment Nitrogen Processes toward Mineralization over Denitrification

Filter-feeding bivalves, like oysters, couple pelagic primary production with benthic microbial processes by consuming plankton from the water column and depositing unassimilated material on sediment. Conceptual models suggest that at low to moderate oyster densities, this deposition can stimulate benthic denitrification by providing denitrifying bacteria with organic carbon and nitrogen (N). While enhanced denitrification has been found at oyster reefs, data from oyster aquaculture are limited and equivocal. This study measured seasonal rates of denitrification, as well as dissimilatory nitrate reduction to ammonium (DNRA), and dissolved inorganic N fluxes at a rack and bag eastern oyster (Crassostrea virginica) aquaculture farm. Consistent with models, denitrification was enhanced within the farm, with an average annual increase of 350% compared to a reference site. However, absolute denitrification rates were low relative to other coastal systems, reaching a maximum of 19.2 μmol m^?2 h^?1. Denitrification appeared to be nitrate (NO₃ ^?) limited, likely due to inhibited nitrification caused by sediment anoxia. Denitrification may also have been limited by competition for NO₃ ^? with DNRA, which accounted for an average of 76% of NO₃ ^? reduction. Consequently, direct release of ammonium (NH₄ ⁺) from mineralization to the water column was the most significant benthic N pathway, with seasonal rates exceeding 900 μmol m^?2 h^?1 within the farm. The enhanced N processes were spatially limited however, with significantly higher rates directly under oysters, compared to in between oyster racks. For commercial aquaculture farms like this, with moderate oyster densities (100–200 oysters m^?2), denitrification may be enhanced, but nonetheless limited by biodeposition-induced sediment anoxia. The resulting shift in the sediment N balance toward processes that regenerate reactive N to the water column rather than remove N is an important consideration for water quality.     

Vermiculite bio-barriers for Cu and Zn remediation: an eco-friendly approach for freshwater and sediments protection

The increase in heavy metal contamination in freshwater systems causes serious environmental problems in most industrialized countries, and the effort to find eco-friendly techniques for reducing water and sediment contamination is fundamental for environmental protection. Permeable barriers made of natural clays can be used as low-cost and eco-friendly materials for adsorbing heavy metals from water solution and thus reducing the sediment contamination. This study discusses the application of permeable barriers made of vermiculite clay for heavy metals remediation at the interface between water and sediments and investigates the possibility to increase their efficiency by loading the vermiculite surface with a microbial biofilm of Pseudomonas putida, which is well known to be a heavy metal accumulator. Some batch assays were performed to verify the uptake capacity of two systems and their adsorption kinetics, and the results indicated that the vermiculite bio-barrier system had a higher removal capacity than the vermiculite barrier (+34.4 and 22.8 % for Cu and Zn, respectively). Moreover, the presence of P. putida biofilm strongly contributed to fasten the kinetics of metals adsorption onto vermiculite sheets. In open-system conditions, the presence of a vermiculite barrier at the interface between water and sediment could reduce the sediment contamination up to 20 and 23 % for Cu and Zn, respectively, highlighting the efficiency of these eco-friendly materials for environmental applications. Nevertheless, the contribution of microbial biofilm in open-system setup should be optimized, and some important considerations about biofilm attachment in a continuous-flow system have been discussed.     

Benthic Carbon Mineralization and Nutrient Turnover in a Scottish Sea Loch: An Integrative In Situ Study

Based on in situ microprofiles, chamber incubations and eddy covariance measurements, we investigated the benthic carbon mineralization and nutrient regeneration in a ~65-m-deep sedimentation basin of Loch Etive, UK. The sediment hosted a considerable amount of infauna that was dominated by the brittle star A. filiformis. The numerous burrows were intensively irrigated enhancing the benthic in situ O₂ uptake by ~50 %, and inducing highly variable redox conditions and O₂ distribution in the surface sediment as also documented by complementary laboratory-based planar optode measurements. The average benthic O₂ exchange as derived by chamber incubations and the eddy covariance approach were similar (14.9 ± 2.5 and 13.1 ± 9.0 mmol m^?2 day^?1) providing confidence in the two measuring approaches. Moreover, the non-invasive eddy approach revealed a flow-dependent benthic O₂ flux that was partly ascribed to enhanced ventilation of infauna burrows during periods of elevated flow rates. The ratio in exchange rates of ΣCO₂ and O₂ was close to unity, confirming that the O₂ uptake was a good proxy for the benthic carbon mineralization in this setting. The infauna activity resulted in highly dynamic redox conditions that presumably facilitated an efficient degradation of both terrestrial and marine-derived organic material. The complex O₂ dynamics of the burrow environment also concurrently stimulated nitrification and coupled denitrification rates making the sediment an efficient sink for bioavailable nitrogen. Furthermore, bioturbation mediated a high efflux of dissolved phosphorus and silicate. The study documents a high spatial and temporal variation in benthic solute exchange with important implications for benthic turnover of organic carbon and nutrients. However, more long-term in situ investigations with like approaches are required to fully understand how environmental events and spatio-temporal variations interrelate to the overall biogeochemical functioning of coastal sediments.     

Differential Effects of Bivalves on Sediment Nitrogen Cycling in a Shallow Coastal Bay

In coastal ecosystems, suspension-feeding bivalves can remove nitrogen though uptake and assimilation or enhanced denitrification. Bivalves may also retain nitrogen through increased mineralization and dissimilatory nitrate reduction to ammonium (DNRA). This study investigated the effects of oyster reefs and clam aquaculture on denitrification, DNRA, and nutrient fluxes (NO _x , NH₄ ⁺, O₂). Core incubations were conducted seasonally on sediments adjacent to restored oyster reefs (Crassostrea virginica), clam aquaculture beds (Mercenaria mercenaria) which contained live clams, and bare sediments from Smith Island Bay, Virginia, USA. Denitrification was significantly higher at oyster reef sediments and clam aquaculture site than bare sediment in the summer; however, DNRA was not enhanced. The clam aquaculture site had the highest ammonium production due to clam excretion. While oyster reef and bare sediments exhibited seasonal differences in rate processes, there was no effect of season on denitrification, or dissimilatory nitrate reduction to ammonium (DNRA) or ammonium flux at the clam aquaculture site. This suggests that farm management practices or bivalve metabolism and excretion may override seasonal differences. When water column nitrate concentration was elevated, denitrification increased in clam aquaculture site and oyster reef sediments but not in bare sediment; DNRA was only stimulated at the clam aquaculture site. This, along with a significant and positive relationship between denitrification and sediment organic matter, suggests that labile carbon limited nitrate reduction at the bare sediment site. Bivalve systems can serve as either net sinks or sources of nitrogen to coastal ecosystems, depending mainly on the type of bivalve, location, and management practices.     

Spatial and Temporal Patterns in <Emphasis Type="Italic">Thalassia testudinum</Emphasis> Leaf Tissue Nutrients at the Chandeleur Islands,Louisiana, USA

Seagrasses are submerged marine plants that are anchored to the substrate and are therefore limited to assimilating nutrients from the surrounding water column or sediment, or by translocating nutrients from adjacent shoots through the belowground rhizome. As a result, seagrasses have been used as reliable ecosystem indicators of surrounding nutrient conditions. The Chandeleur Islands are a chain of barrier islands in the northern Gulf of Mexico that support the only marine seagrass beds in Louisiana, USA, and are the sole location of the seagrass Thalassia testudinum across nearly 1000 km of the coastline from west Florida to central Texas. Over the past 150 years, the land area of the Chandeleur Islands has decreased by over half, resulting in a decline of seagrass cover. The goals of this study were to characterize the status of a climax seagrass species at the Chandeleur Islands, T. testudinum, in terms of leaf nutrient (nitrogen [N] and phosphorus [P]) changes over time, from 1998 to 2015, and to assess potential drivers of leaf nutrient content. Thalassia testudinum leaf nutrients displayed considerable interannual variability in N and P content and molar ratios, which broadly mimicked patterns in annual average dissolved nutrient concentrations in the lower Mississippi River. Hydrological modeling demonstrated the potential for multiple scenarios that would deliver Mississippi River water, and thus nutrients, to T. testudinum at the Chandeleur Islands. Although coastal eutrophication is generally accepted as the proximate cause for seagrass loss globally, there is little evidence that nutrient input from the Mississippi River has driven the dramatic declines observed in seagrasses at the Chandeleur Islands. Rather, seagrass cover along the Chandeleur Islands appears to be strongly influenced by island geomorphological processes. Although variable over time, the often elevated nutrient levels of the climax seagrass species, T. testudinum, which are potentially driven by river-derived nutrient inputs, raises an important consideration of the potential loss of the ecosystem functions and services associated with these declining seagrass meadows.     

Microphytobenthos: The ecological role of the “secret garden” of unvegetated,shallow-water marine habitats. II. role in sediment stability and shallow-water food webs

The microphytobenthos form an important component of all shallow-water ecosystems where enough light reaches the sediment surface to support appreciable primary production. Although less conspicuous than macroalgae or vascular plants, the microphytobenthos can contribute significantly to primary production and can modify habitat characteristics. The microphytobenthos alter sediment properties (e.g., erodibility) both directly, in the extreme forming a mat or scum on the sediment surface, and indirectly by modifying the activities of benthic infauna (e.g., pelletization, burrowing, tube building, and sediment tracking). Carbon dioxide fixed by the microphytobenthos supports higher, grazing trophic levels. These include deposit-feeding and suspension-feeding macrofauna as well as meiofauna and microfauna. Quantitative relations between the feeding and growth rates of macrofauna and the abundance of microphytobenthos and suspended organic matter (i.e., functional responses) are reviewed. Given the current state of knowledge of the direct and indirect interactions involving trophic dynamics, sediment properties, and benthic microalgae, we argue for reductionist studies of particular interactions as distinct entities. This is a prerequisite for the emergence of a comprehensive picture of unvegetated ecosystems and the ability to predict their responses to man’s activities. *** DIRECT SUPPORT *** A01BY074 00005     

Spatial and temporal variations of suspended solid concentrations from 2000 to 2013 in Poyang Lake,China

Concentrations of suspended solids in lakes can affect the latter’s primary productivity and reflect changes in sediment deposition. Determining the temporal and spatial distribution of suspended solid concentrations has important significance in lake water environmental management; this is particularly urgent for Poyang Lake, the largest freshwater lake in China. In this study, suspended solid concentration inversion models for Poyang Lake were created using a semi-empirical method with regression analysis between continuously measured suspended solid concentration data and multi-band moderate-resolution imaging spectroradiometer images for spring, summer, autumn, and winter from 2009 to 2012. The coefficient of determination (R²) is from 0.6 to 0.9 and the average relative error for the accuracy verification was between 10 and 30%. The seasonal distributions of suspended solid concentrations in Poyang Lake from 2000 to 2013 were then obtained using optimal reversal models. The results showed that the seasonal variation in suspended solid concentrations had a “W” shape in which high spring and autumn and low summer and winter values. The suspended solid concentrations increased annually from 2000 to 2013 and were mainly distributed in the northern and central portions of the lake, with lower values along the shorelines. Further analysis indicated that the large difference in water level between the wet and dry seasons is an important factor in explaining these seasonal variations. Moreover, the suspended solid concentrations were poorly correlated with water temperature and chlorophyll-a concentration but more highly correlated with the deferred chlorophyll-a concentration.     

Testing for Sediment Acidification Effects on Within-Season Variability in Juvenile Soft-Shell Clam (<Emphasis Type="Italic">Mya arenaria</Emphasis>) Abundance on the Northern Shore of the Bay of Fundy

Soft-shell clams (Mya arenaria) can serve important ecological roles in intertidal mudflats and are an important economic and recreational resource in the northwestern Atlantic. However, environmental factors affecting newly-settled and juvenile clam abundances within a given settlement season remain uncertain. We conducted a field study assessing relationships between juvenile soft-shell clam abundance and spatial, temporal, and environmental variables in coastal mudflats of the northern shore of the Bay of Fundy. Sediment pH and low-tide water temperature (tide pools) were monitored in situ on a biweekly–monthly basis over the course of the M. arenaria settlement season in 2012 at four study sites to quantify diel variation in sediment pH and temperature. Core samples were also collected to quantify M. arenaria (<6 mm) abundance. Sea-surface and air temperature, and in situ sediment pH and water temperature were then used to predict clam abundance. Sediment pH was spatially and temporally variable, while in situ temperature was stable and declined over the sampling season. Akaike’s information criterion model selection indicated that models incorporating site, date, and air temperature best predicted 2012 recruitment patterns in linear models when all variables were included. When only environmental variables were included, the best linear model included minimum air temperature and sediment pH. When data were averaged across dates for each site to remove potential temporal effects, mean clam abundance displayed a significant positive relationship with sediment pH and sediment grain size, but not minimum air temperature. The results of this study suggest that sediment pH and/or sediment grain size may influence within-season juvenile soft-shell clam abundances across mudflats in the Bay of Fundy. Field experiments at the same (or similar) sites are now needed to confirm the mechanism driving the observed relationships.     

Human alteration of groundwater–surface water interactions (Sagittario River,Central Italy): implication for flow regime,contaminant fate and invertebrate response

Many rivers worldwide are undergoing severe man-induced alterations which are reflected also in changes of the degree of connectivity between surface waters and groundwater. Pollution, irrigation withdrawal, alteration of freshwater flows, road construction, surface water diversion, soil erosion in agriculture, deforestation and dam building have led to some irreversible species losses and severe changes in community composition of freshwater ecosystems. Taking into account the impact of damming and flow diversion on natural river discharge, the present study is aimed at (i) evaluating the effects of anthropogenic changes on groundwater/surface water interactions; (ii) analyzing the fate of nitrogenous pollutants at the floodplain scale; and (iii) describing the overall response of invertebrate assemblages to such changes. Hydrogeological, geochemical and isotopic data revealed short- and long-term changes in hydrology, allowing the assessment of the hydrogeological setting and the evaluation of potential contamination by nitrogen compounds. Water isotopes allowed distinguishing a shallow aquifer locally fed by zenithal recharge and river losses, and a deeper aquifer/aquitard system fed by surrounding carbonate aquifers. This system was found to retain ammonium and, through the shallow aquifer, release it in surface running waters via the hyporheic zone of the riverbed. All these factors influence river ecosystem health. As many environmental drivers entered in action offering a multiple-component artificial environment, a clear relationship between river flow alteration and benthic and hyporheic invertebrate diversity was not found, being species response driven by the combination of three main stressors: ammonium pollution, man-induced changes in river morphology and altered discharge regime.