Role of shellfish hatchery as a reservoir of antimicrobial resistant bacteria

The main aim of this study was to determine the occurrence of resistant bacteria in florfenicol-treated and untreated scallop larval cultures from a commercial hatchery and to characterize some selected florfenicol-resistant strains. Larval cultures from untreated and treated rearing tanks exhibited percentages of copiotrophic bacteria resistant to florfenicol ranging from 0.03% to 10.67% and 0.49–18.34%, respectively, whereas florfenicol resistance among oligotrophic bacteria varied from 1.44% to 35.50% and 3.62–95.71%, from untreated and treated larvae, respectively. Florfenicol resistant microbiota from reared scallop larvae mainly belonged to the Pseudomonas and Pseudoalteromonas genus and were mainly resistant to florfenicol, chloramphenicol, streptomycin and co-trimoxazole. This is the first study reporting antimicrobial resistant bacteria associated to a shellfish hatchery and the results suggest that a continuous surveillance of antimicrobial resistance even in absence of antibacterial therapy is urgently required to evaluate potential undesirable consequences on the surrounding environments.     

Prevalence and distribution of fecal indicator organisms in South Florida beach sand and preliminary assessment of health effects associated with beach sand exposure

Fecal indicator levels in nearshore waters of South Florida are routinely monitored to assess microbial contamination at recreational beaches. However, samples of sand from the surf zone and upper beach are not monitored which is surprising since sand may accumulate and harbor fecal-derived organisms. This study examined the prevalence of fecal indicator organisms in tidally-affected beach sand and in upper beach sand and compared these counts to levels in the water. Since indicator organisms were statistically elevated in sand relative to water, the study also considered the potential health risks associated with beach use and exposure to sand. Fecal coliforms, Escherichia coli, enterococci, somatic coliphages, and F(+)-specific coliphages were enumerated from sand and water at three South Florida beaches (Ft. Lauderdale Beach, Hollywood Beach, and Hobie Beach) over a 2-year period. Bacteria were consistently more concentrated in 100g samples of beach sand (2-23 fold in wet sand and 30-460 fold in dry sand) compared to 100ml samples of water. Somatic coliphages were commonly recovered from both sand and water while F(+)-specific coliphages were less commonly detected. Seeding experiments revealed that a single specimen of gull feces significantly influenced enterococci levels in some 3.1m(2) of beach sand. Examination of beach sand on a micro-spatial scale demonstrated that the variation in enterococci density over short distances was considerable. Results of multiple linear regression analysis showed that the physical and chemical parameters monitored in this study could only minimally account for the variation observed in indicator densities. A pilot epidemiological study was conducted to examine whether the length of exposure to beach water and sand could be correlated with health risk. Logistic regression analysis results provided preliminary evidence that time spent in the wet sand and time spent in the water were associated with a dose-dependent increase in gastrointestinal illness.     

Occurrence and distribution antibiotic resistance of heterotrophic bacteria isolated from a marine beach

Antibiotic resistance of heterotrophic bacteria isolated from a sandy beach in Sopot, at the Southern Baltic Sea coast was determined. The levels of resistance of bacteria to various antibiotics differed considerably. Bacteria inhabiting the middle part of the beach and the dune were most resistant; the least resistant were bacteria isolated from the sea-beach contact zone. Generally, there were no significant differences in antibiotic resistance between pigmented and non-pigmented bacteria. Bacteria isolated from the surface layer of the sand were more resistant to the tested antibiotics than bacteria from the subsurface layers. The majority of bacterial strains were resistant to 3-8 antibiotics. Bacterial resistance to antibiotics was dependent on their chemical structure.     

Luminex detection of fecal indicators in river samples, marine recreational water, and beach sand

Research to understand and remediate coastal pollution is moving toward a multitiered approach in which traditional enumeration of fecal indicators is accompanied by molecular analysis of a variety of targets. Technology that rapidly detects multiple microbial contaminants would benefit from such an approach. The Luminex 100 system is a suspension array that assays multiple analytes rapidly in a single well of a microtiter plate. The ability of the system to simultaneously detect multiple fecal indicating bacteria in environmental samples was tested. Primer/probe sets were designed to simultaneously detect the following fecal indicators: the Bacteroides fragilis group, Enterococcus spp., Escherichia coli and Shigella spp., Bacteroides distasonis, and Ent. faecalis. Specificity and sensitivity of the Luminex probes was tested against laboratory cultures. In addition, sequencing, culture plate testing, and specificity testing with environmental isolates were steps taken to validate the function of the assay with environmental samples. Luminex response to cultures and to environmental samples was consistent with sequencing results, suggesting that the technology has the potential to simultaneously detect multiple targets for coastal water quality applications, particularly as progress is made to efficiently extract DNA from water and sediment matrices.     

Surface selection,adhesion, and retention behavior of marine bacteria on synthetic organic surfaces using self-assembled monolayers and atomic force microscopy

Adhesion of two marine bacteria Shewanella sp. strain T1 and Pseudoalteromonas sp. strain T8, on differently terminated alkanethiolate self-assembled monolayers on gold was investigated. The selected model surfaces—terminated by CH₃, OH, NH₂, COOH, OH-terminated oligo(ethylene glycol), and methyl-terminated oligo(ethylene glycol)—are characterized by contact angle measurement using water, methylene iodide, 1-bromonaphthalene, and formamide. Surface free energies were calculated. Cell counting of the two bacterial strains on the model surfaces after different times revealed differences between the two strains by at least one order of magnitude. For the different surfaces, the bacteria showed comparably small selectivity. Atomic force microscopy images of adhered bacteria showed very different fingerprints on the different surfaces. Electronic supplementary material  Supplementary material is available in the online version of this article at and is accessible to authorized users.     

Cultivable bacteria from bulk water,aggregates, and surface sediments of a tidal flat ecosystem

Most-probable-number (MPN) dilution series were used to enumerate and isolate bacteria from bulk water, suspended aggregates, the oxic layer, and the oxic–anoxic transition zone of the sediment of a tidal flat ecosystem in the southern North Sea. The heterotrophic aerobic bacteria were able to grow on agar-agar, alginate, cellulose, chitin, dried and ground Fucus vesiculosus, Marine Broth 2216, palmitate, and starch. MPN counts of bulk water and aggregate samples ranged between 0.18?×?10¹ and 1.1?×?10⁶ cells per milliliter and those of the sediment surface and the transition zone between 0.8?×?10¹ and 5.1?×?10⁷ cells per gram dry weight. Marine Broth and F. vesiculosus yielded the highest values of all substrates tested and corresponded to 2.3–32% of 4,6-diamidinophenyl indole cell counts. Strains of seven phylogenetic classes were obtained: Actinobacteria, Bacilli, α- and γ-Proteobacteria, Sphingobacteria, Flavobacteria, and Planctomycetacia. Only with agar-agar as substrate could organisms of all seven classes be isolated.     

洞庭湖区域黑线姬鼠（Apodemus agrarius）种群迁入湖滩后的繁殖特征

黑线姬鼠(Apodemus agrarius)是广泛分布于我国的主要农田害鼠之一,2003年三峡工程开始蓄水后洞庭湖湖滩鼠类群落结构发生了演变,黑线姬鼠已大量侵入湖滩,且逐步成为湖滩生境鼠类群落的优势鼠种。动物在新生境中的繁殖状况是了解其对环境适应的关键指标。为掌握黑线姬鼠种群侵入湖滩生境后的适应情况,为其种群动态的预测预报提供科学依据,本文对2003—2020年洞庭湖湖滩和农田生境中捕获的黑线姬鼠繁殖状况进行比较分析,并结合季节动态和生境差异等因子分析了该鼠在繁殖方面对湖滩生境的适应。结果表明:(1)湖滩生境中黑线姬鼠平均繁殖指数(0.71±0.27)低于农田生境(1.21±0.47),在2016—2020年湖滩上黑线姬鼠的繁殖指数为0.99,略高于农田的0.96;(2)湖滩生境较农田生境中的孕鼠在繁殖中偏向有更多的胎仔数,有6只以上胎仔数的孕鼠占比(65.59%)高于农田生境(49.10%);(3)湖滩生境中发现有幼年组的黑线姬鼠参与繁殖,其繁殖指数、参产率、怀孕率及雄鼠的睾丸下降率随年龄递增;(4)春季和秋季是湖滩生境中黑线姬鼠的主要繁殖季节,其繁殖指数、参产率、怀孕率都显著高于...     

Energetics prediction of frequency-dependent suspended sand transport rates on a macrotidal beach

The on–offshore (cross-shore) transport of sand on beaches is highly time-variable, which has made it difficult to model or predict. In this paper, simple energetics modelling is used to compare velocity moment predictions with field observations of suspended sand transport rates. Separate consideration is given to transport associated with the three main frequency-dependent cross-shore transport processes: that associated with the short (incident) waves, that due to the long (infragravity) waves, and transport associated with the mean flow. Direct comparison between the depth-averaged model predictions, and the in-situ point measurements was facilitated by making the first order assumption that the time-averaged suspension profile is exponential and the wave velocity profile is vertically uniform. An appropriate rippled bed roughness was used to provide the drag coefficient in the energetics model and the vertical length scale of the exponential suspension profile. Despite these simple assumptions, comparison of the velocity moment predictions with the field observations of suspended sand fluxes reveals that this approach has the capacity to predict transport magnitudes due to short wave, long wave, and mean flow components to within about one order of magnitude. However, owing to the limitations of the model, the transport direction of the short wave component could not, on occasion, be correctly determined, probably due to ‘reverse’ transport over ripples. © 1998 John Wiley & Sons, Ltd.     

胜利滩海地震勘探采集

胜利滩海地区地理地质条件特殊，勘探程度低、难度大。通过对滩海及极浅海地区采集方法的研究，在改进采集设备的同时，进一步完善了野外观测系统设计，总结了各种激发因素和接收因素，形成一套完整合理的有利于滩海及极浅海地区地震勘探方法，使地震资料的信噪比和分辨率得到了很大提高，从而为地下构造形态研究、油藏描述提供了可靠的基础资料。     

Occurrence and Resistance of Pathogenic Bacteria Along the Tietê River Downstream of São Paulo in Brazil

The load of pathogenic bacteria, their fate and their dangerousness in the Tietê River were assessed along 100 km starting from the city of São Paulo, Brazil. The data were compared with those from two German rivers. High loads of pathogens were found in the Tietê River near the city of São Paulo (Escherichia coli O157:H7, Shigella flexneri, and Shigella boydii), which were absent 30 km downstream of São Paulo. The antibiotic resistances observed in the Tietê river were rather low and decreased after the major input in São Paulo to significantly lower levels about 30 km downstream. While the Brazilian isolates were more susceptible for ampicillin than the German ones, the reverse was observed for gentamycin. For optimal control of infections in humans critical areas where these bacteria survived longer and their elimination mechanisms should be identified as well as the extent and the origin of antibiotic resistance should be determined.     

Influence of nitrate and dissolved organic carbon loading on the interaction of Microcystis aeruginosa and heterotrophic bacteria from hyper-eutrophic lake (Taihu Lake,China)

To investigate the interaction between cyanobacteria and heterotrophic bacteria under gradients of glucose and nitrate, a cyanobacterial strain of Microcystis aeruginosa was grown in microcosms with and without a freshwater bacterial mixture, which was collected from Lake Taihu. Concentrations of glucose (1350, 975, 600, 300, 150, and 37.5 μmol C L⁻¹) and nitrate (150, 300, and 9000 μmol N L⁻¹) were used in a range of combinations giving 9 different treatments of glucose:nitrate. In the microcosm without the bacterial mixture, M. aeruginosa abundance gradually increased with days in all treatments. However, M. aeruginosa had much lower density in some treatments with the bacterial mixture. The difference in M. aeruginosa growth could be explained by competition with bacteria in the cultures in which these were added. The abundance of M. aeruginosa and bacteria when grown together was nearly equal and the number of the bacterial species was highest in the treatment with 300 μmol C L⁻¹ and 150 μmol N L⁻¹. Our results suggest that at this glucose:nitrate ratio M. aeruginosa and the bacterial mixture maintain a balance, and bacteria maintain diversity. In conclusion, we propose that dissolved organic carbon and nitrate availability fundamentally affects the structure as well as stoichiometry of pelagic associations.     

Storm response of a mixed sand gravel beach ridge plain under falling relative sea levels: A stratigraphic investigation using ground penetrating radar

Beach ridge stratigraphy can provide an important record of both sustained coastal progradation and responses to events such as extreme storms, as well as evidence of earthquake induced sediment pulses. This study is a stratigraphic investigation of the late Holocene mixed sand gravel (MSG) beach ridge plain on the Canterbury coast, New Zealand. The subsurface was imaged along a 370 m shore-normal transect using 100 and 200 MHz ground penetrating radar (GPR) antennae, and cored to sample sediment textures. Results show that, seaward of a back-barrier lagoon, the Pegasus Bay beach ridge plain prograded almost uniformly, under conditions of relatively stable sea level. Nearshore sediment supply appears to have created a sustained sediment surplus, perhaps as a result of post-seismic sediment pulses, resulting in a flat, morphologically featureless beach ridge plain. Evidence of a high magnitude storm provides an exception, with an estimated event return period in excess of 100 years. Evidence from the GPR sequence combined with modern process observations from MSG beaches indicates that a palaeo-storm initially created a washover fan into the back-barrier lagoon, with a large amount of sediment simultaneously moved off the beach face into the nearshore. This erosion event resulted in a topographic depression still evident today. In the subsequent recovery period, sediment was reworked by swash onto the beach as a sequence of berm deposit laminations, creating an elevated beach ridge that also has a modern-day topographic signature. As sediment supply returned to normal, and under conditions of falling sea level, a beach ridge progradation sequence accumulated seaward of the storm feature out to the modern-day beach as a large flat, uniform progradation plain. This study highlights the importance of extreme storm events and earthquake pulses on MSG coastlines in triggering high volume beach ridge formation during the subsequent recovery period. © 2019 John Wiley & Sons, Ltd.     

洪泽湖滩地立体开发生态工程研究

运用复合生态学原理,按照洪泽湖源的自然条件,建立垛－塘生态系统工程,作为开发利用的新途径。氢利用与调蓄洪水的矛盾降到了最小程度,达到塘养鱼,坡种草,垛面载桌粮,立体利用,同时研究其他利用途径。经过三年实验证明,经济、社会、生态效益显著。     

Short‐term changes in the plan shape of a sandy beach in response to sheltering by a nearshore mud bank,Cayenne, French Guiana

Montjoly is a headland‐bound embayed sandy beach in Cayenne, French Guiana, that shows long‐term plan shape equilibrium in spite of periodic changes in accretion and erosion that alternately affect either end of the beach. These changes are caused by mud banks that move alongshore from the Amazon. The mechanisms involved in changes in the plan shape of the beach in response to the passage of one of these mud banks were monitored between 1997 and 2000 from airborne video imagery and field work. The beach longshore drift to the northwest, driven by the incident easterly to northeasterly swell usually affecting this coast, became temporarily reversed as the mud bank, migrating from east to west, initially sheltered the southeastern end of the beach. The difference in exposure to waves engendered a negative wave height gradient alongshore towards the southeast, resulting in the setting up of a cell circulation and counter‐active longshore drift from the exposed northwestern sector to the southeast. Sand eroded from the exposed sector accumulated first in the southeastern, and then the central sectors of the beach. The effect of increasing beach sheltering by the mudbank moving west is highlighted on the videographs by an ‘arrested’ pattern of beach shoreline development. The videographs show hardly any changes in beach plan shape since January 1999, due to sheltering of the beach from wave attack by the mud bank. It is expected that the eroded sector will recover in the future as the mud bank passes, leading to re‐establishment of the northwesterly sand drift. This temporally phased bi‐directional drift within the confines of the bounding headlands results in a rare example of mud‐bank‐induced beach rotation, and probably explains the long‐term equilibrium plan shape of Montjoly beach. Copyright © 2002 John Wiley & Sons, Ltd.     

Development of marine water quality criteria for the USA

The US Environmental Protection Agency has developed guidelines for deriving numerical national water quality criteria for the protection of aquatic organisms and their uses. These guidelines provide the method for deriving water quality criteria, including minimum data base requirements, data evaluation procedures, and calculations. The guidelines have been in place for a long time, and states have used them to derive water quality standards for their water bodies. More recent efforts have been directed towards the development of technical guidance based on the concept that bioassessment and biocriteria programs for estuaries and near coastal waters are interrelated and are critical components of comprehensive water resource protection and management. This is a holistic approach to protection and management, integrating biological assessments into traditional chemical and physical evaluations and augmenting the established water quality criteria. The method for deriving water quality criteria and the approach for biocriteria development for marine systems are described.     

Biodegradative potential and characterization of psychrotolerant polychlorinated biphenyl-degrading marine bacteria isolated from a coastal station in the Terra Nova Bay (Ross Sea, Antarctica)

Antarctic marine bacteria were screened for their ability to degrade polychlorinated biphenyls (PCB) as the sole carbon and energy source at both 4 degrees C and 15 degrees C. PCB-degrading isolates (7.1%) were identified by sequencing their 16S rDNA as Pseudoalteromonas, Psychrobacter and Arthrobacter members. One representative isolate per genera was selected for evaluating the biodegradative potential under laboratory scale and phenotypically characterized. Removal of individual PCB congeners was between 35.6% and 79.8% at 4 degrees C and between 0.4% and 82.8% at 15 degrees C. Differences in the removal patterns of PCB congeners were observed in relation to the phylogenetic affiliation: Arthrobacter isolate showed similar biodegradation efficiencies when growing at 4 degrees C and 15 degrees C, while Pseudoalteromonas better degraded PCBs at 15 degrees C. No biodegradation was detected for Psychrobacter isolate at 4 degrees C. Results obtained highlight the occurrence of PCB-degrading bacteria in Antarctic seawater and suggest the potential exploitation of autochthonous bacteria for PCB bioremediation in cold marine environments.     

On the determination of marine magnetotelluric receiver orientation using land magnetic observatory data

Marine magnetotelluric measurements using “free‐fall’’ instruments without effective compasses suffer from the problem of unknown orientation of the receivers at the seafloor. While past works indicate that marine magnetotelluric orientation of the instruments can be estimated by reference to land deployments of known orientation using the transfer tensor method, there is limited published information on how this is implemented in practice. We document this method and propose a set of new time‐ and frequency‐domain approaches to solve this orientation problem of the seafloor receivers. We test these methodologies in onshore and offshore magnetotelluric data whose orientations are well known and apply these techniques to marine magnetotelluric data with unknown orientation. For the controlled tests, both time‐ and frequency‐domain approaches produce overall comparable results. To investigate the effects of the subsurface structure distribution on the orientation process, a dimensionality analysis of a controlled dataset is carried out. In subsequent analysis using the available disoriented marine magnetotelluric data from offshore Brazil and from the Vassouras magnetic observatory on the mainland for remote referencing, frequency‐domain methods yield approximate orientation angles among themselves with low standard deviation each. Time‐domain results are consistent for most cases but differ from frequency‐domain results for some situations.     

Antibiotic resistance of bacteria isolated from shrimp hatcheries and cultural ponds on Donghai Island, China

The resistance of bacteria to 12 different antibiotics was investigated in shrimp farms on Donghai Island, China. Antibiotic-resistant bacteria were found to be widespread in shrimp farms, indicating a high environmental risk. Further, significant differences were found in bacterial strains among farms (ANOVA, p < 0.05), showing resistance to antibiotics such as ampicillin, trimethoprim, compound sinomi, tetracycline, chloramphenicol and cefazolin. No significant differences in antibiotic resistance were found among 6 hatcheries evaluated in this study (ANOVA, p > 0.05), between exalted and traditional shrimp ponds (ANOVA, p > 0.05), and between cultural ponds and corresponding control water source sites (T-test, p > 0.05). In cultural ponds, no significant difference in bacterial resistance to antibiotics was found between water and sediment (T-test, p > 0.05), and antibiotic resistance of bacteria from water showed a significant positive correlation with that from sediment (p < 0.05). Therefore, our study indicates that bacterial multiple antibiotic resistance (MAR) is more widespread in shrimp hatcheries than ponds.     

Molecular characterizations of chloramphenicol- and oxytetracycline-resistant bacteria and resistance genes in mariculture waters of China

In order to gain an understanding of the diversity and distribution of antimicrobial-resistant bacteria and their resistance genes in maricultural environments, multidrug-resistant bacteria were screened for the rearing waters from a mariculture farm of China. Both abalone Haliotis discushannai and turbot Scophthalmus maximus rearing waters were populated with abundant chloramphenicol-resistant bacteria. These bacteria were also multidrug resistant, with Vibriosplendidus and Vibriotasmaniensis being the most predominant species. The chloramphenicol-resistance gene cat II, cat IV or floR could be detected in most of the multidrug-resistant isolates, and the oxytetracycline-resistance gene tet(B), tet(D), tet(E) or tet(M) could also be detected for most of the isolates. Coexistence of chloramphenicol- and oxytetracycline-resistance genes partially explains the molecular mechanism of multidrug resistance in the studied maricultural environments. Comparative studies with different antimicrobial agents as the starting isolation reagents may help detect a wider diversity of the antimicrobial-resistant bacteria and their resistance genes.