BIOB: A mathematical model for the biodegradation of low solubility hydrocarbons

Modeling oil biodegradation is an important step in predicting the long term fate of oil on beaches. Unfortunately, existing models do not account mechanistically for environmental factors, such as pore water nutrient concentration, affecting oil biodegradation, rather in an empirical way. We present herein a numerical model, BIOB, to simulate the biodegradation of insoluble attached hydrocarbon. The model was used to simulate an experimental oil spill on a sand beach. The biodegradation kinetic parameters were estimated by fitting the model to the experimental data of alkanes and aromatics. It was found that parameter values are comparable to their counterparts for the biodegradation of dissolved organic matter. The biodegradation of aromatics was highly affected by the decay of aromatic biomass, probably due to its low growth rate. Numerical simulations revealed that the biodegradation rate increases by 3–4 folds when the nutrient concentration is increased from 0.2 to 2.0 mg N/L.     

Lab tests on the biodegradation of chemically dispersed oil should consider the rapid dilution that occurs at sea

Most crude oils spread on open water to an average thickness as low as 0.1 mm. The application of dispersants enhances the transport of oil as small droplets into the water column, and when combined with the turbulence of 1 m waves will quickly entrain oil into the top 1 m of the water column, where it rapidly dilutes to concentrations less than 100 ppm. In less than 24 h, the dispersed oil is expected to mix into the top 10 m of the water column and be diluted to concentrations well below 10 ppm, with dilution continuing as time proceeds. Over the multiple weeks that biodegradation takes place, dispersed oil concentrations are expected to be below 1 ppm. Measurements from spills and wave basin studies support these calculations. Published laboratory studies focused on the quantification of contaminant biodegradation rates have used concentrations orders of magnitude greater than this, as it was necessary to ensure the concentrations of hydrocarbons and other chemicals were higher than the detection limits of chemical analysis. However, current analytical methods can quantify individual alkanes and PAHs (and their alkyl homologues) at ppb and ppm levels. To simulate marine biodegradation of dispersed oil at dilute concentrations commonly encountered in the field, laboratory studies should be conducted at similarly low hydrocarbon concentrations.     

Effects of nutrients on crude oil biodegradation in the upper intertidal zone

To enhance biodegradation, nutrients in the form of slow-release fertilizer (SRF) were applied to oil-contaminated microcosms (3%, v/v) which simulated intertidal environmental systems. Although nutrient concentrations in the interstitial water were not proportional to those in amended SRF, microbial activity, growth of oil-degrading microorganisms, and oil-degradation rate were closely related to the concentration of nutrients in the interstitial water. Adding nutrients at higher dose (microcosm I, 144.4 mg C/kg sand/day, versus microcosm II, 8.5 mg C/kg sand/day) had a positive effect on oil degradation rate, which was especially obvious during the early phase of treatment. Use of pristane, phytane, and nor-hopane as biomarkers enabled the detection of significant treatment differences in hydrocarbon biodegradation, which were not reliable enough when the data were normalized to sand mass.     

Analysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by DGGE

A microbial consortium was obtained by enrichment culture of sea water samples collected from Botan oil port in Xiamen, China, using the persistent high concentration of a mixture of polycyclic aromatic hydrocarbons enrichment strategy. Denaturing gradient gel electrophoresis (DGGE) was used to investigate the bacterial composition and community dynamic changes based on PCR amplification of 16S rRNA genes during batch culture enrichment. Using the spray-plate method, three bacteria, designated as BL01, BL02 and BL03, which corresponded to the dominant bands in the DGGE profiles, were isolated from the consortium. Sequence analysis showed that BL01, BL02 and BL03 were phylogenetically close to Ochrobactrum sp., Stenotrophomonas maltophilia and Pseudomonas fluorescens, respectively. The degradation of benzo(a)pyrene (BaP), a model high-molecular-weight polycyclic aromatic hydrocarbon (HMW PAH) compound was investigated using individual isolates, a mixture of the three isolates, and the microbial consortium (BL) originally isolated from the oil port sea water. Results showed that the order of degradative ability was BL > the mixture of the three isolates > individual isolates. BL degraded 44.07% of the 10 ppm BaP after 14 days incubation, which showed the highest capability for HMW PAH compound degradation.Our results revealed that this high selective pressure strategy was feasible and effective in enriching the HMW PAH-degraders from the original sea water samples.     

Effects of temperature and wave conditions on chemical dispersion efficacy of heavy fuel oil in an experimental flow-through wave tank

The effectiveness of chemical dispersants (Corexit 9500 and SPC 1000) on heavy fuel oil (IFO180 as test oil) has been evaluated under different wave conditions in a flow-through wave tank. The dispersant effectiveness was determined by measuring oil concentrations and droplet size distributions. An analysis of covariance (ANCOVA) model indicated that wave type and temperature significantly (p < 0.05) affected the dynamic dispersant effectiveness (DDE). At higher temperatures (16 °C), the test IFO180 was effectively dispersed under breaking waves with a DDE of 90% and 50% for Corexit 9500 and SPC 1000, respectively. The dispersion was ineffective under breaking waves at lower temperature (10 °C), and under regular wave conditions at all temperatures (10-17 °C), with DDE < 15%. Effective chemical dispersion was associated with formation of smaller droplets (with volumetric mean diameters or VMD ? 200 μm), whereas ineffective dispersion produced large oil droplets (with VMD ? 400 μm).     

Biodegradation and transport of benzene,toluene, and xylenes in a simulated aquifer: comparison of modelled and experimental results

Both laboratory experiments and numerical modelling were conducted to study the biodegradation and transport of benzene–toluene–xylenes (BTX) in a simulated semi‐confined aquifer. The factors incorporated into the numerical model include advection, hydrodynamic dispersion, adsorption, and biodegradation. The various physico‐chemical parameters required by the numerical model were measured experimentally. In the experimental portion of the study, BTX compounds were introduced into the aquifer sand. After the contaminants had been transported through the system, BTX concentrations were measured at 12 equally spaced wells. Subsequently, microorganisms obtained from the activated sludge of a sewage treatment plant and cultured in BTX mixtures were introduced into the aquifer through the 12 sampling wells. The distribution data for BTX adsorption by the aquifer sand form a nonlinear isotherm. The degree of adsorption by the sand varies, depending on the composition of the solute. The degradation time, measured from the time since the bacteria were added to the aquifer until a specific contaminant was no longer detectable, was 35–42 h for BTX. The dissolved oxygen, after degradation by BTX compounds and bacteria, was consumed by about 40–60% in the entire simulated aquifer; thus the aerobic conditions were maintained. This study provides insights for the biodegradation and transport of BTX in aquifers by numerical modelling and laboratory experiments. Experimental and numerical comparisons indicate that the results by Monod degradation kinetics are more accurate than those by the first‐order degradation kinetics. Copyright © 2002 John Wiley & Sons, Ltd.     

Glyphosate persistence in seawater

Glyphosate is one of the most widely applied herbicides globally but its persistence in seawater has not been reported. Here we quantify the biodegradation of glyphosate using standard “simulation” flask tests with native bacterial populations and coastal seawater from the Great Barrier Reef. The half-life for glyphosate at 25 °C in low-light was 47 days, extending to 267 days in the dark at 25 °C and 315 days in the dark at 31 °C, which is the longest persistence reported for this herbicide. AMPA, the microbial transformation product of glyphosate, was detected under all conditions, confirming that degradation was mediated by the native microbial community. This study demonstrates glyphosate is moderately persistent in the marine water under low light conditions and is highly persistent in the dark. Little degradation would be expected during flood plumes in the tropics, which could potentially deliver dissolved and sediment-bound glyphosate far from shore.     

Gravity-geoelectric joint inversion over the Potiguar rift basin, NE Brazil

A joint inversion of gravity and geoelectrical data has been performed along a 100 km long profile across the oil-bearing Potiguar rift basin in NE Brazil. The integrated approach is based on the connection between density and resistivity of a sedimentary sequence through the porosity. Seventy-one gravity stations and twenty-nine vertical electrical soundings were carried out across the central part of the Early Cretaceous basin in the Precambrian Borborema Province to apply the proposed joint inversion. Both the physical properties and geometry of the multilayer deep model were well constrained by a wide set of prior information obtained by surface mapping, geophysical logs and seismic sections. The results reveal a rift architecture formed by a 5000 m thick half-graben structure separated by an intervening basement high and an extensive adjacent platform with a sedimentary infill of about 300 m thick. The calculated model shows geometries for the sedimentary density/resistivity interfaces in agreement with the seismostratigraphic sequences revealed by seismic section, representing a substantial improvement in comparison with previously published gravity models.     

Isolation and characterization of a novel hydrocarbon-degrading bacterium Achromobacter sp. HZ01 from the crude oil-contaminated seawater at the Daya Bay,southern China

Microorganisms play an important role in the biodegradation of petroleum contaminants, which have attracted great concern due to their persistent toxicity and difficult biodegradation. In this paper, a novel hydrocarbon-degrading bacterium HZ01 was isolated from the crude oil-contaminated seawater at the Daya Bay, South China Sea, and identified as Achromobacter sp. Under the conditions of pH 7.0, NaCl 3% (w/v), temperature 28 °C and rotary speed 150 rpm, its degradability of the total n-alkanes reached up to 96.6% after 10 days of incubation for the evaporated diesel oil. Furthermore, Achromobacter sp. HZ01 could effectively utilize polycyclic aromatic hydrocarbons (PAHs) as its sole carbon source, and could remove anthracene, phenanthrene and pyrence about 29.8%, 50.6% and 38.4% respectively after 30 days of incubation. Therefore, Achromobacter sp. HZ01 may employed as an excellent degrader to develop one cost-effective and eco-friendly method for the bioremediation of marine environments polluted by crude oil.     

A case study of damages of the Kandla Port and Customs Office tower supported on a mat–pile foundation in liquefied soils under the 2001 Bhuj earthquake

A case study is presented of the interaction between the bending due to laterally spreading forces and axial-load induced settlement on the piled foundations of the Kandla Port and Customs Tower located in Kandla Port, India, during the 2001 Bhuj earthquake. The 22 m tall tower had an eccentric mass at the roof and was supported on a piled-raft foundation that considerably tilted away as was observed in the aftermath of the earthquake. The soil at the site consists of 10 m of clay overlaid by a 12 m deep sandy soil layer. Post-earthquake investigation revealed the following: (a) liquefaction of the deep sandy soil strata below the clay layer; (b) settlement of the ground in the vicinity of the building; (c) lateral spreading of the nearby ground towards the sea front. The foundation of the tower consists of 0.5 m thick concrete mat and 32 piles. The piles are 18 m long and therefore passes through 10 m of clayey soil and rested on liquefiable soils. Conventional analysis of a single pile or a pile group, without considering the raft foundation would predict a severe tilting and/or settlement of the tower eventually leading to a complete collapse. It has been concluded that the foundation mat over the non-liquefied crust shared a considerable amount of load of the superstructure and resisted the complete collapse of the building.     

Measuring the effect of structural connectivity on the water dynamics in heterogeneous porous media using speedy neutron tomography

The temporal and spatial distribution of water within a porous medium is affected by the medium’s structure, i.e., the spatial arrangement of its constituents. To analyze structural effects on the fluid dynamics, we measured the 3D water content distribution in a heterogeneous sand column during two drainage-wetting cycles using neutron transmission tomography. The sample with a volume of 105 cm³ contained 101 cubes of fine and 49 cubes of coarse sand with particles ranging from 0.01 to 0.05 and 0.03 to 0.09 cm, respectively. The pressure at the lower boundary was determined by the water reservoir positioned between 7 and 39 cm below the top of the column. The duration of one complete 3D scanning with a spatial resolution of 127 μm was 56 s. The signal to noise ratio of the measurements was low due to the short exposure time in the neutron beam, but it was possible to quantify the water content in the individual cubes and hence the effect of structure on macroscopic water distribution. Continuous structures of coarse sand drained faster than coarse sand without connection to the upper boundary. During the initial wetting phase, cubes of coarse sand material completely embedded in the fine material remained water unsaturated due to air entrapment. The effect of the coarse sand connectivity was analyzed in two-dimensional numerical simulations based on Richards equation. In contrast to the measurements, no effect of structure connectivity was found. The coarse sand cubes embedded within the fine matrix drain as quickly as the coarse sand cubes arranged in a continuous channel due to the model assumption of a continuous air phase.     

Effects of chronic exposure to dispersed oil on selected reproductive processes in adult blue mussels (Mytilus edulis) and the consequences for the early life stages of their larvae

Mussels (Mytilus edulis) were continuously exposed to dispersed crude oil (0.015-0.25 mg/l) for 7 months covering the whole gamete development cycle. After 1 month exposure to 0.25 mg oil/l, the level of alkali-labile phosphates (ALP) and the volume density of atretic oocytes in female gonads were higher than those in the gonads of control females, indicating that oil affected the level of vitellogenin-like proteins and gamete development. Spawning of mussels was induced after 7 months oil exposure. Parental oil exposure did not affect subsequent fertilization success in clean seawater but this was reduced in 0.25 mg oil/l. Parental exposure to 0.25 mg oil/l caused both slow development and a higher percentage of abnormalities in D-shell larvae 2 days post-fertilization; reduced growth 7 days post-fertilization. These effects were greatly enhanced when larval stages were maintained at 0.25 mg oil/l. Similar studies are warranted for risk assessment prognosis.     

A mechanistic study on the photodegradation of Irgarol-1051 in natural seawater

The kinetics of the photoinduced degradation and transformation of the antifouling booster biocide, Irgarol-1051, in natural coastal seawater was studied. The measured first-order rate constant for the degradation of Irgarol-1051 was 4.02 ± 0.1 × 10⁻⁴ h⁻¹, while the rate constant for the formation of 2-methylthio-4-tert-butylamino-6-amino-s-triazine (M1), the most dominant degradation product of Irgarol-1051, was 4.6 ± 0.1 × 10⁻⁵ h⁻¹. This considerably slower rate suggested that the transformation of Irgarol-1051 to M1 may not be the predominant pathway of the photodegradation process. During the photodegradation study, a new s-triazine species was observed in the degradation mixtures which, together with M1, appeared immediately upon photolysis and continued to accumulate in the degradation mixture throughout the entire study duration. This is in contrast to the behaviour of the recently identified degradation product of Irgarol-1051, 3-[4-tert-butylamino-6-methylthiol-s-triazin-2-ylamino]- propionaldehyde (M2), which was only detected in the degradation mixture after a long induction period. High-resolution tandem mass spectrometric analysis hinted that the new degradation product (M4) may possess a terminal alcohol and is likely to be an N-allylic alcohol derivative of M1. This suggests that M4 may, indeed, be a precursor of M2 via redox transformation at its N-allylic alcohol functionality.     

Crude oil bioremediation field experiment in the Sea of Japan

Experimental bioremediation of crude oil was conducted for approximately 3 months in the intertidal zone of the Sea of Japan, Hyogo Prefecture. Artificial mixtures of weathered Arabian light crude oil and sand taken from the experimental site were wrapped in polyester net envelopes. The envelopes were placed in drum-shaped acrylic vessels with perforated sides to facilitate seawater exchange. The vessels were laid in the intertidal area. Slow release nitrogen and phosphorus synthetic fertilizer granules were added to the oil-sand mixtures in three different amounts. Some oil-sand mixtures were unfertilized controls. The oil-sand mixtures were periodically sampled and changes in the composition of the residual oils were monitored. Oil samples were subjected to gas chromatography coupled with mass spectrometry for analysis of some representative semi-volatile aliphatic and aromatic compounds. All values for each analyte were normalized against that of hopane to evaluate the extent of oil biodegradation. Significant increases in the concentrations of both nitrogen and phosphorus were found in the fertilized sections in accordance with the amounts of added fertilizers. Although significant natural attenuation of oil was observed in the unfertilized sections, fertilization stimulated the degradation rate of the oil in the early stage of the experimental term. The extent of the oil biodegradation increased as the amount of added fertilizer increased. However, the final degradation efficiencies for each oil component in the fertilized sections were not significantly different from those in the unfertilized sections, and the degradation of each oil component had almost ceased after 6 weeks. We conclude that excessive amounts of macronutrients are required to accelerate oil biodegradation and that fertilization is only effective in the early stages.     

The influence of nearshore sand bank dynamics on shoreline evolution in a macrotidal coastal environment,Calais, northern France

Analyses of shoreline and bathymetry change near Calais, northern coast of France, showed that shoreline evolution during the 20th century was strongly related with shoreface and nearshore bathymetry variations. Coastal erosion generally corresponds to areas of nearshore seabed lowering while shoreline progradation is essentially associated with areas of seafloor aggradation, notably east of Calais where an extensive sand flat experienced seaward shoreline displacement up to more than 300 m between 1949 and 2000. Mapping of bathymetry changes since 1911 revealed that significant variation in nearshore morphology was caused by the onshore and alongshore migration of a prominent tidal sand bank that eventually welded to the shore. Comparison of bathymetry data showed that the volume of the bank increased by about 10×10⁷ m³ during the 20th century, indicating that the bank was acting as a sediment sink for some of the sand transiting alongshore in the coastal zone. Several lines of evidence show that the bank also represented a major sediment source for the prograding tidal flat, supplying significant amounts of sand to the accreting upper beach. Simulation of wave propagation using the SWAN wave model (Booij et al., 1999) suggests that the onshore movement of the sand bank resulted in a decrease of wave energy in the nearshore zone, leading to more dissipative conditions. Such conditions would have increased nearshore sediment supply, favoring aeolian dune development on the upper beach and shoreline progradation. Our results suggest that the onshore migration of nearshore sand banks may represent one of the most important, and possibly the primary mechanism responsible for supplying marine sand to beaches and coastal dunes in this macrotidal coastal environment.     

Analysis of bacterial diversity and metals in produced water, seawater and sediments from an offshore oil and gas production platform

Produced water is one of the largest waste products routinely discharged into the ocean from offshore oil and gas platforms. This study analyzed bacterial communities and metals in the produced water, surrounding seawater, and sediment around the Thebaud platform. The bacterial community within the produced water was different from the seawater (SAB = 13.3), but the discharge had no detectable effect on the bacterial communities in the seawater (SAB > 97). In contrast, genomic analysis of sediments revealed that the bacterial community from 250 m was different (SAB = 70) from other locations further from the discharge, suggesting that the produced water had a detectable effect on the bacterial community in the sediment closest to the discharge. These near-field sediments contained elevated concentrations of manganese and iron that are associated with the produced water effluent. The results suggested that the discharge of produced water has influenced the bacterial community structure of sediments adjacent to the platform.     

Hydrodynamics and sediment-transport in the nearshore of Poverty Bay, New Zealand: Observations of nearshore sediment segregation and oceanic storms

Nearshore regions act as an interface between the terrestrial environment and deeper waters. As such, they play important roles in the dispersal of fluvial sediment and the transport of sand to and from the shoreline. This study focused on the nearshore of Poverty Bay, New Zealand, and the processes controlling the dispersal of sediment from the main source, the Waipaoa River. Hydrodynamics and sediment-transport in water shallower than 15 m were observed from April through mid-September 2006. This deployment afforded observations during 3-4 periods of elevated river discharge and 5 dry storms.Similar wind, river discharge, wave, current, and turbidity patterns were characterized during three of the wet storms. At the beginning of each event, winds blew shoreward, increasing wave heights to 2-3 m within Poverty Bay. As the cyclonic storms moved through the system the winds reversed direction and became seaward, reducing the local wave height and orbital velocity while river discharge remained elevated. At these times, high river discharge and relatively small waves enabled fluvially derived suspended sediment to deposit in shallow water. Altimetry measurements indicated that at least 7 cm was deposited at a 15 m deep site during a single discharge event. Turbidity and seabed observations showed this deposition to be removed, however, as large swell waves from the Southern Ocean triggered resuspension of the material within three weeks of deposition. Consequently, two periods of dispersal were associated with each discharge pulse, one coinciding with fluvial delivery, and a second driven by wave resuspension a few weeks later. These observations of nearfield sediment deposition contradict current hypotheses of very limited sediment deposition in shallow water offshore of small mountainous rivers when floods and high-energy, large wave and fast current, oceanic conditions coincide.Consistently shoreward near-bed currents, observed along the 10 m isobath of Poverty Bay, were attributed to a combination of estuarine circulation, Stokes drift, and wind driven upwelling. Velocities measured at the 15 m isobath, however, were directed more alongshore and diverged from those at the 10 m isobath. The divergence in the currents observed at the 10 and 15 m locations seemed to facilitate segregation of coarse and fine sediment, with sand transported near-bed toward the beach, while suspended silts and clays were exported to deeper water.     

Analysis of repeated-load laboratory tests on buried plastic pipes in sand

This paper describes a laboratory model test carried out on high-density polyethylene (HDPE), small diameter pipes buried in trenches, which subjected to repeated loadings to simulate the vehicle loads. Deformation of the pipe was recorded at eight points on the circumference of the tested pipes to measure the radial deformations and detect cross-sectional pipe profiles. Also settlement of the soil surface during the test up to 1000 cycles of loadings was recorded, until its value become stable or the excessive settlement was happened. The parameters varied in the testing program include height of buried depth, relative density of the sand and intensity of stress on the soil surface. The influence of various repeated loads (with magnitude of 250, 400 and 550 kPa) at relative densities of 42%, 57% and 72% in different embedded depth of 1.5–3 times of pipe diameter were investigated. Based on the results, in medium and dense sand relative density, the pipe embedded in depth of 3.0D and 2.0D, respectively, mostly remained undamaged (the maximum value of VDS is less than 5%) and increased the safety of buried pipes under different magnitude of repeated loads. The records of the pipe deformation and settlement of the soil surface due to the repeated loads have been compared in different conditions. These values increase rapidly during the initial loading cycles by a rate decreasing significantly as the number of cycles increase. The influence of the first cycle was also found to be one of the main behavioral characteristics of buried pipes under repeated loads. The ratio of deformation of pipe at first cycle to last cycle changes from 0.60 to 0.85 in different of tests. Finally for the obtained results, a non-linear power model has been developed to estimate the vertical diametral strain of buried pipe and settlement of the soil surface based on the model test data. It should be noted that only one type of pipe and one type of sand are used in laboratory tests.     

Evaluating crude oil chemical dispersion efficacy in a flow-through wave tank under regular non-breaking wave and breaking wave conditions

Testing dispersant effectiveness under conditions similar to that of the open environment is required for improvements in operational procedures and the formulation of regulatory guidelines. To this end, a novel wave tank facility was fabricated to study the dispersion of crude oil under regular non-breaking and irregular breaking wave conditions. This wave tank facility was designed for operation in a flow-through mode to simulate both wave- and current-driven hydrodynamic conditions. We report here an evaluation of the effectiveness of chemical dispersants (Corexit^® EC9500A and SPC 1000^™) on two crude oils (Medium South American [MESA] and Alaska North Slope [ANS]) under two different wave conditions (regular non-breaking and plunging breaking waves) in this wave tank. The dispersant effectiveness was assessed by measuring the water column oil concentration and dispersed oil droplet size distribution. In the absence of dispersants, nearly 8-19% of the test crude oils were dispersed and diluted under regular wave and breaking wave conditions. In the presence of dispersants, about 21-36% of the crude oils were dispersed and diluted under regular waves, and 42-62% under breaking waves. Consistently, physical dispersion under regular waves produced large oil droplets (volumetric mean diameter or VMD ? 300 μm), whereas chemical dispersion under breaking waves created small droplets (VMD ? 50 μm). The data can provide useful information for developing better operational guidelines for dispersant use and improved predictive models on dispersant effectiveness in the field.     

Spatial variability in community structure of Dictyoceratida sponges across Torres Strait,Australia

Spatial variability in community structure of dictyoceratid sponges (class Demospongiae; order Dictyoceratida) was examined on coral reefs in Torres Strait, an archipelago of islands and reefs between northern Queensland, Australia, and Papua New Guinea. Dictyoceratid sponge abundances and environmental factors were recorded at four locations, separated by 50–220 km. Each location was subdivided into 5–7 sites, each ?2 km apart. At each site, four 50×2 m belt transects were quantitatively surveyed, recording the dictyoceratid numbers, substrate type (rock, rubble and sand), water clarity, degree of reef slope and depth. Dictyoceratid abundance was similar among locations over large spatial scales, averaging 15.5 individuals per 100 m², but varied significantly among sites within particular locations (i.e. small scale heterogeneity). Twenty-three dictyoceratid species were recorded in Torres Strait, with approximately half (12/23) found in only one location. The canonical correspondence analysis determined that the measured environmental factors explained only 26% of the spatial variation. Cluster analysis revealed a complex dictyoceratid community structure with similarities among neighbouring sites and among sites separated by hundreds of kilometres. Conversely the abundance and composition of dictyoceratids could vary greatly between neighbouring sites 2 km apart and on the same reef complex. The results of this study suggest that spatial variability of dictyoceratid sponges in Torres Strait is influenced by a combination of environmental, biological and stochastic processes.