Ecological status assessment in the lower Eo estuary (Spain). The challenge of habitat heterogeneity integration: a benthic perspective

The implementation of the European Water Framework Directive (WFD) requires evaluation of the ecological status (ES) of benthic communities in coastal and transitional water systems, and the intercalibration of assessment methodologies therefore becomes a research challenge. Our aim was to test the suitability of applying the M-AMBI index to assess the status of the Eo estuary (northern coast of Spain). Our results showed that M-AMBI was influenced by the natural variability of benthic communities, and presented an apparent dependence on habitat characteristics. Consequently, the definition of homogeneous areas in transitional water systems should be based on the salinity gradient combined with other factors. To achieve an accurate ES assessment, habitat-specific reference conditions should then be defined prior to the application of M-AMBI; this necessitates dividing an estuary into several sections, which may be classified as different ESs. From this perspective, a novel approach to integrate habitat heterogeneity in a global ES assessment was tested.     

Towards the application of the Water Framework Directive in Italy: Assessing the potential of benthic tools in Adriatic coastal transitional ecosystems

The potential of four benthic indices (AMBI/M-AMBI, BENTIX, BITS) was assessed in Italian coastal transitional ecosystems. The community composition showed a strong dominance of lagoonal, tolerant species, and out of more of 400 species found, only about 40 taxa were dominant. The full agreement of the four indices on an undegraded (Good or better) or degraded (Moderate or worse) status occurred only in 32.3% of stations. This study evidenced that BENTIX is inappropriate for eutrophic Adriatic lagoons, and that in such environments M-AMBI classification is actually too much dependent on diversity and richness, and seems unable to capture some peculiarities of benthic assemblages in transitional waters. AMBI and BITS gave similar classifications despite the different level of taxonomic identification needed. The unmodified use of these indices might impair accurate assessment of ecological quality status and decision-making on the managers’ point of view.     

Critical appraisal on the identification of Reference Conditions for the evaluation of ecological quality status along the Emilia-Romagna coast (Italy) using M-AMBI

According to the European Water Framework Directive, the ecological status (ES) of a water body is determined by comparing observation data with undisturbed Reference Conditions (RCs). Defining RCs is crucial when evaluating the ES of a water body as it strongly affects the final outcome of any index application. Identifying RCs by observing real sites is not feasible in many marine environments, such as the Emilia-Romagna coast (Italy, N-Adriatic Sea). We used a statistical approach on a large dataset to derive RCs for the application of the benthic index M-AMBI in this area. We then applied M-AMBI to samples collected along a gradient of presumed environmental disturbance. The results put 14.8% of the Emilia-Romagna samples in “High” ES, 60.2% in “Good”, 23.0% in “Moderate” and 2.0% in “Poor”, showing a spatial gradient of improving quality. These results are in agreement with the extensive ecological knowledge available for this area.     

A revisitation of TRIX for trophic status assessment in the light of the European Water Framework Directive: application to Italian coastal waters

The trophic status classification of coastal waters at the European scale requires the availability of harmonised indicators and procedures. The composite trophic status index (TRIX) provides useful metrics for the assessment of the trophic status of coastal waters. It was originally developed for Italian coastal waters and then applied in many European seas (Adriatic, Tyrrhenian, Baltic, Black and Northern seas). The TRIX index does not fulfil the classification procedure suggested by the WFD for two reasons: (a) it is based on an absolute trophic scale without any normalization to type-specific reference conditions; (b) it makes an ex ante aggregation of biological (Chl-a) and physico-chemical (oxygen, nutrients) quality elements, instead of an ex post integration of separate evaluations of biological and subsequent chemical quality elements. A revisitation of the TRIX index in the light of the European Water Framework Directive (WFD, 2000/60/EC) and new TRIX derived tools are presented in this paper. A number of Italian coastal sites were grouped into different types based on a thorough analysis of their hydro-morphological conditions, and type-specific reference sites were selected. Unscaled TRIX values (UNTRIX) for reference and impacted sites have been calculated and two alternative UNTRIX-based classification procedures are discussed. The proposed procedures, to be validated on a broader scale, provide users with simple tools that give an integrated view of nutrient enrichment and its effects on algal biomass (Chl-a) and on oxygen levels. This trophic evaluation along with phytoplankton indicator species and algal blooms contribute to the comprehensive assessment of phytoplankton, one of the biological quality elements in coastal waters.     

Assessing reference conditions and physico-chemical status according to the European Water Framework Directive: a case-study from the Basque Country (Northern Spain)

The European Water Framework Directive requires the development of new and accurate methodologies, addressing the assessment of the physico-chemical status of transitional and coastal waters; these are considered by the Directive as the supporting elements for the final evaluation of the Ecological Quality Status.

This contribution develops new approaches in the determination of the physico-chemical status, solving some problems detected in previous contributions, i.e.: (a) fitting the classification of water bodies and typologies, by means of the stretching of the typologies, according to the natural salinity gradient of types; (b) defining reference conditions, based upon the new approach to typologies, (c) proposing accurate multivariate methodologies, in determining the physico-chemical status of the transitional and coastal waters, based upon the defined typologies and references; and (d) discussion of the results obtained by reference to methodological aspects and water quality evolution in the Basque Country, Spain (as a case-study), during the last decade.     

Development of a River Macrophyte Index (RMI) for assessing river ecological status

We present the process of developing a macrophyte based index (River Macrophyte Index – RMI) for assessing river ecological status, that would be applicable for rivers with moderate to high water alkalinity, flowing over low slope terrain. A reference value and boundary values were determined for five ecological classes. The relation between the developed index and two existing indices, the Reference Index (RI) and the Trophic Index of Macrophytes (TIM), and selected environmental variables was established. The RMI is based on species composition and abundance from 208 sampling sites being in reference or good hydromorphological conditions and differing in the catchment land use. The percentage of natural areas in the sub-catchment was used for classifying macrophyte taxa into 5 ecological groups. 65 plant taxa, of which 47 were identified as indicator taxa, were included in the analysis. To assess the ecological status of a river site, the presence of at least 3 indicator taxa is necessary, otherwise the assessment is considered inconclusive. RMI is expected to indicate multiple pressures on the river, including trophic level. The developed index and RI and TIM indices differed in relation to slope, distance to source and catchment size.     

Palaeoecology, reference conditions and classification of ecological status: the EU Water Framework Directive in practice

The European Union's Water Framework Directive (WFD) requires that all Member States within the European Union determine reference conditions for aquatic ecosystems to provide a baseline against which to measure the effects of past and present activities. Reference conditions are subsequently used to classify the ecological status of European waters. The decisions regarding environmental status will be important future elements in the management of European coastal waters. We have developed a number of classification scenarios for total nitrogen (TN) in the overlying waters of the southern part of Roskilde Fjord, Denmark, taking as our basis a palaeoecological reconstruction of fluctuations in TN between 1850 and 1995. We present a provisional classification scheme for the ecological status of Roskilde Fjord, sensu the WFD. Decision(s) regarding the deviation from reference conditions will give a wide range of apparent ecological status from good, through moderate and poor, to bad depending upon the definition of an acceptable deviation from reference conditions. The determination of an acceptable deviation will ultimately be a political decision, and will result in a wide range in the protection of coastal waters in Europe. There is still, however, an urgent need for a sound scientific documentation of the various scenarios for the implementation of the WFD.     

An intercalibration of classification metrics of benthic macroinvertebrates in coastal and transitional ecosystems of the Eastern Mediterranean ecoregion (Greece)

Three coastal and three lagoonal sites located in Greece (Eastern Mediterranean) were selected to test and intercalibrate classification methods developed for benthic invertebrates ecological quality assessments. These methods were developed for the purposes of the European Water Framework Directive which is a European legislation adopted for the protection and improvement of the coastal and transitional waters. However, through testing these methods, this work addresses in general the issue of determining and comparing the ecological status of the coastal and transitional macrobenthic communities in Eastern Mediterranean ecosystems. Among methods tested were the biotic indices AMBI and BENTIX, the multivariate method M-AMBI and the biomass size structure index ISD in lagoonal sites only. ISD index is a rather new method developed especially for transitional ecosystems and in this contribution its performance is intercalibrated with the other benthic classification metrics. Comparison and intercalibration of the indices results in assessing the ecological quality status (EQS) are presented graphically and statistically performing the Kappa analysis. Results of the comparison and the indices' performance are evaluated based on the knowledge of the sites' environmental condition and baseline studies. Based on an extended dataset of coastal and transitional ecosystems benthic invertebrates, an evaluation of each index performance and effectiveness is attempted through an insight and comparative analysis of each methods' structure and design. Results showed that in these Eastern Mediterranean coastal sites the BENTIX index seems to give a more biologically relevant classification and gives a higher confidence level regarding the classification compared to the other indices. In the coastal areas AMBI showed a tendency for the "good" class classification, while the factorial M-AMBI gave more consistent results with the BENTIX. In the lagoonal sites the biotic indices tested were not proved efficient enough and the biomass based ISD index proved more appropriate for these ecosystems. AMBI showed the highest agreement with the ISD which is due to the better performance of this index in the slightly and moderately polluted lagoons, compared to the other indices. However, AMBI failed to classify the polluted lagoon, in which case the BENTIX gave a more relevant classification.     

Phytoplankton pigments and epifluorescence microscopy as tools for ecological status assessment in coastal and estuarine waters, within the Water Framework Directive

Inverted microscopy is widespread employed for the analysis of phytoplankton composition within water quality monitoring networks. However, the analysis at the lowest taxonomical level is not always required for ecological status assessment. In addition, inverted microscopy can underestimate the small phytoplankton, and not always distinguish photoautotrophic from heterotrophic cells. In this study, as alternative tools, epifluorescence microscopy and High Performance Liquid Chromatography (HPLC) were employed to characterize phytoplankton communities within waters of different trophic condition. Epifluorescence microscopy confirmed its effectiveness to count the small phytoplankton. Furthermore, significant correlations between nutrients of anthropogenic origin and nanoplankton abundances were found. However, this technique resulted very time-consuming. HPLC together with the CHEMTAX program was more appropriate than inverted microscopy, in terms of cost-effectiveness. Also, the main variability patterns observed in the phytoplankton community structure by HPLC coincided with previous findings in the study area. Nevertheless, a rapid screening at the inverted microscope is recommended.     

Development of a fish-based index to assess the ecological quality of transitional waters: The case of French estuaries

The Water Framework Directive requires the assessment of the ecological status of transitional waters considering the fish component. An original methodology, based on a pressure-impact approach, was established to develop a multimetric fish-based index to characterize the ecological quality of French estuaries. An index of contamination, based on the chemical pollution affecting aquatic systems, was used as a proxy of anthropogenic pressure. The fish metric selection was based on their response to disturbances tested via statistical models (generalized linear models) taking into account sampling strategy and estuarine features. Four metrics, for which discriminating responses to level of pressure were demonstrated, were retained to constitute the estuarine multimetric fish index. This new tool appeared particularly relevant to detect the contamination effects on fish communities in estuaries. It could help managers to take decisions in order to maintain or reach the good status required by the Water Framework Directive for 2015.     

Type-specific and indicator taxa of phytoplankton as a quality criterion for assessing the ecological status of Finnish boreal lakes

Key issues in the implementation of the Water Framework Directive are classification of lakes using biological quality parameters and type-specific reference conditions. This work is one of three studies considering different metrics of phytoplankton in the classification of ecological status. Phytoplankton was studied in a total of 55 Finnish boreal lakes, including 32 reference lakes. We tested the suitability of taxonomic composition and abundance of phytoplankton groups for biological classification. We also preliminarily determined the type-specific taxa for the studied lakes. The type-specific taxa for reference conditions are coincidently the indicator species/taxa for high ecological status. Interestingly, some taxa type-specific for impacted oligo-humic lakes proved to be the type-specific taxa for humic reference lakes. The pressure of human impact was observed not only as increase of biomass but also as changes in the species composition. The phytoplankton composition indicated the ecological status of impacted lakes moderately well. There was some variation in the indications given by different algal groups, probably due to the preliminary class boundaries used. However, the preliminary combination of indicative parameters to estimate the ecological status of the studied impacted lakes was in general in accordance with earlier classification of water quality in Finnish lakes.     

Ecological classification of macrophytes and phytobenthos for rivers in Germany according to the water framework directive

A new assessment system for macrophytes and phytobenthos in German rivers meeting the requirements of the Water Framework Directive (WFD) of the European Community is described. Biocoenotic types based on biological, chemical and hydromorphological data from over 200 river sites covering the main ecoregions, hydromorphological stream types and degradation forms have been defined. For developing a classification system the quality element macrophytes and phytobenthos was divided into three components: macrophytes, benthic diatoms and remaining phytobenthos. For macrophytes seven types including one subtype, for benthic diatoms 14 types including three subtypes and for the remaining phytobenthos five river types were identified. The benthic vegetation at reference condition was described for most of the river types. Degradation is characterised as deviation in benthic vegetation species composition and abundance from the reference biocoenosis. For classification in five ecological status classes, several metrics were developed and used in combination with existing indices. For some of the described river types additional investigations are necessary before a classification system can be developed.     

Macrophytes and phytobenthos as indicators of ecological status in German lakes — a contribution to the implementation of the water framework directive

A new assessment system for macrophytes and phytobenthos in German lakes according to the Water Framework Directive of the European Community is described. Based on biological, chemical and hydromorphological data from about 100 lake sites covering the main ecoregions, hydromorphological lake types and degradation forms, biocoenotic types could be defined. For developing a classification system the quality element macrophytes and phytobenthos was divided into two components: macrophytes and benthic diatoms. For macrophytes 4 and for benthic diatoms 4 lake types were identified. The benthic vegetation at reference conditions is described and degradation is characterised as deviation in benthic vegetation species composition and abundance from the reference biocoenosis. For classification in five ecological status classes, several metrics were developed and used in combination with existing indices. For a few of the described lake types further investigations are necessary before a classification can be developed.     

Assessment of the ecological status of Mediterranean French coastal waters as required by the Water Framework Directive using the Posidonia oceanica Rapid Easy Index: PREI

This paper describes the PREI (Posidonia oceanica Rapid Easy Index), a method used to assess the ecological status of seawater along Mediterranean French coasts. The PREI was drawn up according to the requirements of the Water Framework Directive (WFD 2000/60/EC) and was tested on 24 and 18 stations in PACA (Provence-Alpes-Côtes d’Azur) and Corsica, respectively. The PREI is based on five metrics: shoot density, shoot leaf surface area, E/L ratio (epiphytic biomass/leaf biomass), depth of lower limit, and type of this lower limit. The 42 studied stations were classified in the first four levels of status: high, good, moderate and poor. The PREI values ranged between 0.280 and 0.847; this classification is in accordance with our field knowledge and with our knowledge of the literature. The PREI was validated regarding human pressure levels (r² = 0.74). (http://eurex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2000:327:0001:0072:EN:PDF).     

Depth limit of littoral vegetation in a storage reservoir: A case study of Lipno Reservoir (Czech Republic)

Controlling factors for the occurrence of littoral macrophyte species were investigated in Lipno Reservoir (area: 46.5 km²; z_mean: 6.6 m; z_max: 22 m; water residence time: 0.6 yr), a dam impoundment with moderately fluctuating water level (amplitude up to 4 m) which is used for hydropower and downstream flow augmentation. The options of supporting littoral macrophyte growth for improving the reservoir's ecological potential were evaluated according to the European Water Framework Directive's definitions. Macrophytes were examined at 115 sites, each 50 m long, along the whole reservoir perimeter at regular 1 km intervals. Sites were surveyed for their phytocoenology, shore morphometry, pedological characteristics of substrate, transparency of water, and anthropogenic impacts. Most of the sites were characterised by a wide eulittoral zone with a tenuous cover of terrestrial hydrophilic, amphibious, and/or emergent macrophytes and a low proportion of silt, clay, and organic fractions in the substrate. Infralittoral submerged and floating-leaved macrophytes were restricted to a few sites within the mouths of the main and side tributaries where transparency was maintained by clear inflow water independent of reservoir pool fluctuations. Water transparency was low (1-2 m) compared to annual changes of water level (2 to >3 m). Multivariate statistical analysis (DCA, CCA) confirmed relationships between the occurrence of macrophyte species and morphological and substrate characteristics of the shore. Improvement of macrophyte cover and establishment of vital infralittoral zones requires improvements in water transparency and changes in reservoir operation, i.e., limiting the range of water level fluctuation to less than ca. 1 m.     

Multimetric Macroinvertebrate Index Flanders (MMIF) for biological assessment of rivers and lakes in Flanders (Belgium)

The European Water Framework Directive requires that member states assess all their surface waters based on a number of biological elements, including macroinvertebrates. Since 1989, the Flemish Environment Agency has been using the Belgian Biotic Index for assessing river water quality based on macroinvertebrates. Throughout the years, the Belgian Biotic Index has proven to be a reliable and robust method providing a good indication of general degradation of river water and habitat quality. Since the Belgian Biotic Index does not meet all the requirements of the Water Framework Directive, a new index, the Multimetric Macroinvertebrate Index Flanders (MMIF) for evaluating rivers and lakes was developed and tested. This index was developed in order to provide a general assessment of ecological deterioration caused by any kind of stressor, such as water pollution and habitat quality degradation. The MMIF is based on macroinvertebrate samples that are taken using the same sampling and identification procedure as the Belgian Biotic Index. The index calculation is a type-specific multimetric system based on five equally weighted metrics, which are taxa richness, number of Ephemeroptera, Plecoptera and Trichoptera taxa, number of other sensitive taxa, the Shannon-Wiener diversity index and the mean tolerance score. The final index value is expressed as an Ecological Quality Ratio ranging from zero for very bad ecological quality to one for very good ecological quality. The MMIF correlates positively with dissolved oxygen and negatively with Kjeldahl nitrogen, total nitrogen, ammonium, nitrite, total phosphorous, orthophosphate and biochemical and chemical oxygen demand. This new index is now being used by the Flemish Environment Agency as a standard method to report about the status of macroinvertebrates in rivers and lakes in Flanders within the context of the European Water Framework Directive.     

Influence of seasonal variability in benthic invertebrate community structure on the use of biotic indices to assess the ecological status of a Portuguese estuary

The present study focused on the use of benthic invertebrate communities to assess the ecological quality of a Portuguese estuary characterized by strong seasonal changes and with eutrophication problems. Seasonal benthic samples were collected during a flood year and the methodology proposed by the WFD Portuguese group was used to classify benthic assemblages into five different quality classes. Factor analysis was applied to classify stations based on their physical-chemical status. Different classifications were obtained with different indices and among seasons and there was low agreement between indices and index-season interactions. Diversity indices were better correlated to eutrophication related variables than AMBI and ABC method. Predictable responses of benthic indices to anthropogenic stress symptoms were stronger during the dry period.     

Functions and ecological status of eight Italian lagoons examined using biological traits analysis (BTA)

The soft-bottom communities of eight Italian lagoons were analyzed for eight biological traits (feeding, mobility, adult life habitat, body size, life span, reproductive technique, type of larva and reproductive frequency) in order to identify the dominant traits in different transitional environments. We considered the ecological quality status (EcoQS) of the stations, assessed by two biotic indices, AMBI and Bentix. Stations were categorized into EcoQS classes to investigate the relationship between biological functions and ecological quality. The results indicate that the variability of the data was governed by traits linked to resource utilization rather than to life cycle. Lagoons affected by chronic disturbance displayed a poor functional composition, which usually corresponded to poor EcoQS in some cases, correlations between ecological groups and traits modalities were ecologically relevant; however, classes of EcoQS were found to be relatively independent from the functional structure of the considered stations.