ALUMINUM CONTENT OF TEA LEAVES AND FACTORSAFFECTING THE UPTAKE OF ALUMINUM FROMSOIL INTO TEA LEAVES

1 INTRODUCTIONAluminum, the most abundant metallic elementwithin the lithosphere (7% -- 8% by weight), occursgenerally only in combined form: as an oxide inban-cite, the primary ore, and in complex aluminosilicates sueh as micas and feldspars (HEM, 1986;LINDSAY, 1979). These compounds of Al, nearlyunavailable to plants, dissolve to form the hydrated ionAl(H,O),' (written A13 for simplicity) or hydrolysisproducts of this ion under acidic conditions. The Alions bind to canon-excha…     

Preservation of organic matter in soils of a climo-biosequence in the Main Range of Peninsular Malaysia

Limited information is available about factors of soil organic carbon(SOC) preservation in soils along a climo-biosequence. The objective of this study was to evaluate the role of soil texture and mineralogy on preservation of SOC in the topsoil and subsoil along a climo-biosequence in the Main Range of Peninsular Malaysia. Soil samples from the A and B-horizons of four representative soil profiles were subjected to particle-size fractionation and mineralogical analyses including X-ray diffraction and selective dissolution. The proportion of SOC in the 250-2000 μm fraction(SOC associated with coarse sand) decreased while the proportion of SOC in the 53 μm fraction(SOC associated with clay and silt)increased with depth. This reflected the importance of the fine mineral fractions of the soil matrix for SOC storage in the subsoil. Close relationships between the content of SOC in the 53 μm fraction and the content of poorly crystalline Fe oxides [oxalate-extractable Fe(Fe_o) – pyrophosphate-extractable Fe(Fe_p)] and poorly crystalline inorganic forms of Al [oxalateextractable Al(Al_o) – pyrophosphate-extractable Al(Al_p)] in the B-horizon indicated the importance of poorly crystalline Fe oxides and poorly crystalline aluminosilicates for the preservation of SOC in the Bhorizon. The increasing trend of Fe_o-Fe_p and Al_o-Al_p over elevation suggest that the importance of poorly crystalline Fe oxides and poorly crystalline aluminosilicates for the preservation of SOC in the Bhorizon increased with increasing elevation. This study demonstrates that regardless of differences in climate and vegetation along the studied climobiosequence, preservation of SOC in the subsoil depends on clay mineralogy.     

The effect of salinity on Fucus ceranoides (Ochrophyta,Phaeophyceae) in the Mondego River (Portugal)

Algae(and their extracts) are increasingly important for pharmaceutical applications due to the diversity of useful compounds they contain. The genus Fucus contains one of the most studied species,Fucus vesiculosus. The species F. ceranoides differs from the others of the genus by presenting longitudinal air-vesicles and a capacity to survive at low salinities. It is an alga that inhabits the Mondego River estuary(Portugal), at the southern limit of its distribution, and can serve as a role model to understand the effect of a salt gradient on the production of bioactive compounds. We assessed the phenolic content and antioxidant activity of different F. ceranoides extracts(e.g. methanolic, aqueous and polysaccharide) prepared from samples harvested from two different zones to evaluate if the adaptation of F. ceranoides to different salinity levels influenced its chemical composition. The antioxidant activity of the extracts was determined using 1,2-diphenyl-picrylhydrazyl(DPPH) and 2.2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radicals. These assays demonstrated that the methanolic extract of lyophilized F. ceranoides that grew at low salinities was the most bioactive, i.e. DPPH(IC_(50)=50.39 μg/mL) and ABTS(TEAC=2.42). The total phenolic content(Folin-Ciocalteu method) and the methanolic extract of the lyophilized F. ceranoides collected from a low salinity habitat exhibited the highest phenolic content(PGE=49.48 μg/mg of lyophilized extract)amongst those sampled. Thin layer chromatography(TLC) and Fourier transform infrared spectroscopy(FTIR) were used for the identification of compounds in the extracts. This characterization allowed confirmation that the various extracts contained almost the same compounds but with notable quantitative differences. Based on these results, we conclude that there were differences in the quantity of the compounds due to the effect of salinity. The drying methods used were also found to have influenced the quality of the extracted compounds.     

江浙典型茶园土壤有机质空间异质性分析

茶园土壤有机质分布有明显的空间性,如何凭借有限的采样点数据预测研究区的有机质分布,对了解茶叶种植区土壤状况、指导茶园经营管理、提高茶叶品质和产量有重要意义。本文对江浙4种名茶种植区,利用描述性统计,以及地统计学中的半方差函数模型、空间插值等方法对比分析了茶园土壤有机质的空间异质性。研究结果表明:(1)4个研究区的土壤有机质平均水平均较高,适合茶叶生长,且浙江省2个茶叶种植区的有机质平均水平高于江苏省2个茶叶种植区的有机质水平;(2)半方差函数模型拟合表明,溪龙乡种植区有机质含量表现出中等空间相关性,而其他3个种植区表现出强烈的空间相关性;(3)各种植区土壤有机质的空间分布以结构性因子(土壤理化性质、地形地貌)为基础,受人类活动、经营模式、茶园管理等随机性因子影响,整体趋势上表现出局部变异的特征。     

Tolerance of ciliated protozoan Paramecium bursaria （Protozoa,Ciliophora） to ammonia and nitrites

The tolerance to ammonia and nitrites in freshwater ciliate Paramecium bursaria was measured in a conventional open system. The ciliate was exposed to different concentrations of ammonia and nitrites for 2h and 12h in order to determine the lethal concentrations. Linear regression analysis revealed that the 2h-LC50 value for ammonia was 95.94 mg/L and for nitrite 27.35 mg/L using probit scale method (with 95% confidence intervals). There was a linear correlation between the mortality probit scale and logarithmic concentration of ammonia which fit by a regression equation y=7.32x-9.51 (R^2=0.98; y, mortality probit scale; x, logarithmic concentration of ammonia), by which 2 h-LC50 value for ammonia was found to be 95.50 mg/L. A linear correlation between mortality probit scales and logarithmic concentration of nitrite is also followed the regression equation y=2.86x 0.89 (R^2=0.95; y, mortality probit scale; x, logarithmic concentration of nitrite). The regression analysis of toxicity curves showed that the linear correlation between exposed time of ammonia-N LC50 valueand ammonia-N LC50 value followed the regression equation y=2 862.85e-0.08x (R^2=0.95; y, duration of exposure to LC50 value; x, LC50 value), and that between exposed time of nitrite-N LC50 value and nitrite-N LC50 value followed the regression equation y = 127.15e-0.13x (R^2=0.91; y, exposed time of LC50 value; x, LC50 value). The results demonstrate that the tolerance to ammonia in P. bursaria is considerably higher than that of the larvae or juveniles of some metozoa, e.g. cultured prawns and oysters. In addition, ciliates, as bacterial predators, are likely to play a positive role in maintaining and improving water quality in aquatic environments with high-level ammonium, such as sewage treatment systems.     

ALFISOLS AND CLOSELY RELATED SOILS IN CHINA

Alfisols are important soils in China. They occupy about 1.25 million km3, or about 13% of the land area. In the current Chinese system of soil classification, burozem, yellow-brown earths, Baijiang (Planosol) soils and parts of drab soils. They are mostly forested soils with an estimated 5-13 t / ha · yr of organic matter returned to the soils from temperate mixed conifer and broad-leaved forest. In terms of elemental bio-cycling, Ca is prominent.In a comparison of 30 profiles the average ratio of clay (B/ A) was 1.47 for Cryoboralfs and Eutroboralfs; 1.88 for Hapludaifs and 2.53 for Paleudalfs. From Eutroboralfs to Paleudalfs the average gain (or loss) in clay during soil development is about a factor of seven.The moisture regimes vary considerably between Hapludaifs, Cryoboralfs, and associated Cryaquepts, but the amount of water is always enough to cause significant leaching. In the weathering, and pedogenesis processes TiO2, MgO and Fe2O3 are accumulated, respectively, in both A and BA horizons; b     

Free radical scavenging abilities of polypeptide from Chlamys farreri

1 INTRODUCTION Active oxygen free radicals can damage cell structure, even kill the cells, causing aging and cer- tain serious diseases (Bors et al., 1989). Usually, the production and scavenging of active oxygen free radicals are in balance in healthy hu…     

Transformation of Carbon and Nitrogen by Earthworms in the Decomposition Processes of Broad-leaved Litters

Earthworms are the important constituents in the decayed food web and the main ecological conditioners in the process of decomposition and nutrient mineralization. The transformation of organic carbon (C) and total nitrogen (N) in the broad-leaved litters ingested by earthworms was researched by means of a laboratory experiment. Experimental samples were collected from broad-leaved Korea Pine mixed forest in Liangshui National Natural Reserve (47°10′50″N, 128°53′20″E) in the northeastern Xiao Hinggan Mountains of Northeast China. The contents of organic C and total N in earthworms, leaf litters and earthworm faeces were analyzed. Results show that the organic C content was in the fol- lowing order: leaf litters>faeces>earthworms, while total N content was contrary to that of the organic C. The organic C contents in the different leaf litters were in the following order: Tilia amurensis>Betula costata>Acer mono, whereas the total N contents in the different leaf litters were: Betula costata>Tilia amurensis>Acer mono. The contents of organic C and total N in the faeces from the different leaf litters were almost consistent with the contents of the leaf litters. After the leaf litters were ingested by earthworms, the organic C, which was transformed to increase earthworms' weights, ac- counted for 3.90%-13.31% of the total ingestion by earthworms, while that in the earthworm faeces accounted for 6.14%-13.70%. The transformed organic C through the other metabolism (e.g., respiration) of earthworms accounted for 75.04%-89.92%. The ingested organic C by earthworms was mostly used for metabolic activities. The N ingested by earthworms was less than organic C. It is estimated that 37.08% of total N was transformed to increase the earthworm's weight, 19.97% into earthworm faeces and 47.86% for the consumption of the earthworm's activities. The earthworms not only increased the content of organic C and total N in the soil, but also decreased the values of C/N in the soil and leaf litters. Earthworms play a major role in the leaf litters' decomposition and transformation.     

Effect of soil salinity on cold tolerance of mangrove Kandelia candel

Analysis of cold tolerance on mangroveKandelia candel leaf growing in different soil salinity along Jiulong River Estuary in South China showed that the cold tolerance decreased as the increase of soil salinity. The lethal temperatures ofK. candel leaf were −10.4, −9.9 and −8.6 °C in Liaodong, Baijiao and Aotou, respectively. Under 1–2°C cold stress treatment on detached leaves ofK. candel, their caloric value gradually decreased, while electrolyte leakage gradually increased. The leaf's caloric value and electrolyte leakage in Aotou with higher soil salinity varied more largely than those in Liaodong with lower soil salinity. InK. candel leaf, total water content lowered a little, bound water content rose significantly and free water content dropped significantly with duration of cold stress. At the same time, reduction sugar, soluble sugar and starch content gradually decreased and sucrose content gradually increased. Bound water, free water and sucrose content inK. candel leaf from Aotou with higher soil salinity changed more slowly than those from Liaodong with lower soil salinity, but reduction sugar, soluble sugar and starch content inK. candel leaf from Aotou had faster variations than those from Liaodong. These data indicated that soil salinity can reduce cold tolerance ofK. candel leaf by increasing negative effect of salt ions in cell membrane, inhibiting variations of water content, and aggravating consumption of material and energy. Supported by the Found for Outstanding Young Teacher, the Ministry of Education, China.     

Effect of germanium dioxide on growth of Spirulina platensis

This study on the effect of different concentrations of germanium dioxide (GeO₂) on the specific growth rate (SGR), pigment contents, protein content and amino acid composition ofSpirulina platensis showed that Ge was not the essential element of this alga; that GeO₂ could speed up growth and raise protein content ofS. platensis, and could possibly influence the photosynthesis system. The concentration range of GeO₂ beneficial to growth ofS. platensis is from 5–100mg/l. GeO₂ is proposed to be utilized to remove contamination byChlorella spp. usually occurring in the cultivation ofSpirulina.     

Land use effects on soil organic carbon,microbial biomass and microbial activity in Changbai Mountains of Northeast China

Land use changes are known to alter soil organic carbon （SOC） and microbial properties, however, information about how conversion of natural forest to agricultural land use as well as plantations affects SOC and microbial properties in the Changbai Moun- tains of Northeast China is meager. Soil carbon content, microbial biomass carbon （MBC）, basal respiration and soil carbon mineraliza- tion were studied in five selected types of land use： natural old-growth broad-leaved Korean pine mixed forest （NF）; spruce plantation （SP） established following clear-cutting of NF; cropland （CL）; ginseng farmland （GF） previously under NF; and a five-year Mongolian oak young forest （YF） reforested on an abandoned GF, in the Changbai Mountains of Northeast China in 2011. Results showed that SOC content was significantly lower in SP, CL, GF, and YF than in NF. MBC ranged from 304.4 mg/kg in CL to 1350.3 mg/kg in NF, which was significantly higher in the soil of NF than any soil of the other four land use types. The SOC and MBC contents were higher in SP soil than in CL, GF, and YF soils, yielding a significant difference between SP and CL. The value of basal respiration was also higher in NF than in SP, CL, GF, and YF. Simultaneously, higher values of the metabolic quotient were detected in CL, GF, and YF soils, indicat- ing low substrate utilization of the soil microbial community compared with that in NF and SP soil. The values of cumulative mineral- ized carbon and potentially mineralized carbon （Co） in NF were significantly higher than those in CL and GF, while no significant dif- ference was observed between NF and SP. In addition, YF had higher values of Co and C mineralization rate compared with GF. The results indicate that conversion from NF into agricultural land （CL and GF） uses and plantation may lead to a reduction in soil nutrients （SOC and MBC） and substrate utilization efficiency of the microbial community. By contrast, soils below SP were more conducive to the preservation of soil organic matter, which was reflected in the comparison of microbial indicators among CL, GF, and YF land uses. This study can provide data for evaluating soils nutrients under different land use types, and serve as references for the rational land use of natural forest in the study area.     

Micromorphology and quality attributes of the loess derived soils affected by land use change: A case study in Ghapan watershed, Northern Iran

In order to study the effects of different land vegetative covers on soil quality attributes, a loess hill slope was selected in eastern Golestan Province, Ghapan watershed, Iran. Four profiles in four land uses, including Quercus natural forest; Pinus artificial forest; Cupressus artificial forest and a cultivated land, were studied. Results showed that MWD was significantly different in the studied land uses, and it varied between 1.6 mm in Quercus natural forest and o.31 mm in cultivated land use. The lowest CEC, microbial respiration rate and organic carbon were 28.4 cmol·kg^1, 177 μgCO2·g^-1·day^-1 and 1.32 % found in cultivated land use, respectively. The organic matter was considerably higher content in the forest areas than that of cultivated land use. The studies on soil profile development revealed that the natural forest soils were highly developed. The soils of the Quercus natural forest were classified as Calcic Haploxeralfs with a well developed argillie horizon unlike the cultivated soils which showed the minimum development and classified as Typic Xerorthents. The soils of the artificial forests had both mollic epipedons and were classified as Typic Calcixerolls with moderate profile development. Micromorphological studies revealed that argillic horizons had speckled and partly crystallitic b-fabric in the natural forest indicating the high landscape stability. In contrast, the crystallitic b-fabric of other land uses shows the absence of enough leaching of carbonate and the subsequent migration of clay particles indicating the unstable conditions and high soil erosion. Intense erosion of the surface horizons of cultivated land use has resulted in the outcropping of the subsurface carbonate rich horizons preventing soil development.     

Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration

Bryopsis hypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli.In this study,the photosynthetic responses during dehydration of both the thalli and protoplasts isolated from B.hypnoides were measured using a Dual-PAM(pulse amplitude modulation)-100 fluorometer.The results show that the photosynthetic rates of B.hypnoides thalli were maintained for an initial period,beyond which continued desiccation resulted in reduced rates of PSI and PSII.However,the photosynthetic performances of the isolated protoplasts dehydrated in air(CO2 concentration 600±700 mg/L) showed a slight increase of Y(II) at 20% water loss,but the rates decreased thereafter with declining water content.When protoplasts were dehydrated in CO2 deficient conditions(CO2 concentration 40±80 mg/L),the values of Y(II) declined steadily with increased dehydration without an initial rise.These results indicated that the thalli and isolated protoplasts of this alga can utilize CO2 in ambient air effectively,and the photosynthetic performances of the isolated protoplasts were significantly different from that of the thalli during dehydration.Thus the protoplasts may be an excellent system for the study of stress tolerance.     

雷州半岛南部土壤肥力综合评价及空间变异

通过测定雷州半岛南部845个耕地土壤样品pH、有机质、全氮、有效磷、速效钾、碱解氮的含量,采用层次分析法确定各肥力评价指标权重,应用模糊数学法对该区域耕地土壤肥力进行综合评价,并利用ArcGIS 9.2软件对土壤肥力空间变异进行分析.结果表明:雷州半岛南部土壤有机质、全氮、有效磷、速效钾、碱解氮平均值分别为26.18 g/kg、1.48 g/kg 、34.63 mg/kg、158.57 mg/kg、133.17 mg/kg;土壤速效磷的变异系数最高,为103.14%,属强变异性,有机质、速效钾、碱解氮、全氮的变异系数属中等变异性,pH值的变异系数最小.土壤肥力总体水平处于中等偏低;从空间分布来看,土壤肥力中部高,东北、西南部相对较低     

基于土壤地球化学特征的茶叶种植适宜性评价分析——以日照巨峰镇为例

为响应国家乡村振兴的号召,进一步提升提高日照市巨峰镇土地质量地球化学调查成果的科技转化潜力,本文综合运用层次分析法、SPSS软件系统聚类法、内梅罗综合指数法、变异系数法等方法,在查明研究区土壤地球化学背景值、评价研究区的土壤肥力、评价研究区的茶叶生化品质、研究研究区茶叶生化特征的影响因素的基础上,对研究区内土壤的茶叶宜种性进行综合评价。结合研究区的生产建设实际及已有茶园分布情况,将研究区大致划分为最适宜种植区、一般适宜种植区和不适宜种植区三大类。其中适宜种植区4720hm2,一般适宜种植区7793hm2,不适宜种植区3480hm2。本次工作为研究区茶叶种植区扩建提供了科学指导,进一步提升了土地质量地球化学调查成果的科技转化水平。     

Analysis of the genetic diversity and differentiation of <Emphasis Type="Italic">Fenneropenaeus penicillatus</Emphasis> populations using AFLP technology

Fenneropenaeus penicillatus (redtail shrimp) is an important marine commercial animal in China. Recently, its resources have been depleted rapidly as a result of, for example, over-exploitation and environmental degradation of spawning grounds. Therefore, we analyzed the genetic diversity and differentiation of nine wild populations of F. penicillatus of China (Ningde, Lianjiang, Putian, Xiamen, Quanzhou, Zhangpu, Dongshan, Nanao, and Shenzhen populations) by amplified fragment length polymorphism (AFLP) technology, to provide genetic information necessary for resource protection, rejuvenation, artificial breeding, and sustainable use of the resource. Eight AFLP primer pairs were used for amplification, and 508 bands were detected among the populations. The results show that the percentage of polymorphic loci (P) ranged from 41.34% to 63.58%; the Nei’s gene diversity (H) of the populations was 0.119 4-0.230 5; and Shannon’s Information Index (I) was 0.184 1-0.342 5. These genetic data indicate that the genetic diversity of F. penicillatus was high. The genetic differentiation coefficient (GST=0.216 2) and gene flow (Nm=1.812 4) show that there was a high level of genetic differentiation and a moderate level of gene flow among populations. More studies on the genetic differentiation mechanism of F. penicillatus along the south-eastern coast of China need to be conducted to find more effective scientific protection strategies for the conservation of F. penicillatus genetic resources.     

The functional relation between ion concentration in water vapor chemistry and the bond parameter in quantum chemistry

ImODUcrION'Watervaporchenhatry"asanewsdencewasestablisedbyGu'slaboratorybasedontheirdiscoveryoftheruleof'witCr-watervaporconstanttransferoftracemetalionsinthenaturalwaterCyde';andwasprovedbyamodelexperimntofsea-airtransfer(Gu,l988,l99l,Gueta1.,l982,l986).Thertilehasnotorreationwiththethermody-nawhrsofthetracephySicochdricalsyStem(Gu,l99l,Guetal.,l984).ThispaperpresentSthefuflctionrelating'witCfvaporchedtry"ionconamtrationtothe'huantumchedtry"bondparametCr.FUimONThedatausedinthecurvea…     

Starvation metabolism of two common species of chironomids in Biandantang Lake

1 INTRODUCTION Chironomid larvae are main groups in most aquatic ecosystems, playing a crucial ecological role in decomposition of detritus and material ex- changes between water column and the sediment (Liang et al., 1995a, b; Chen, et al., 1982). They a…     

Soil organic carbon contents,aggregate stability,and humic acid composition in different alpine grasslands in Qinghai-Tibet Plateau

Alpine grassland soils on Qinghai-Tibet Plateau store approximately 33.5 Pg of organic carbon(C) at 0–0.75 m depth and play an important role in the global carbon cycle.We investigated soil organic C(SOC),water-soluble organic C(WSOC),easily oxidizable organic C(EOC),humic C fractions,aggregate-associated C,aggregate stability,and humic acid(HA) composition along an east-west transect across Qinghai-Tibet Plateau,and explored their spatial patterns and controlling factors.The contents of SOC,WSOC,EOC,humic C fractions and aggregate-associated C,the proportions of macroaggregates(2-0.25) and micro-aggregates(0.25-0.053 mm),and the aggregate stability indices all increased in the order alpine desert alpine steppe alpine meadow.The alkyl C,O-alkyl C,and aliphatic C/aromatic C ratio of HA increased as alpine desert alpine meadow alpine steppe,and the trends were reverse for the aromatic C and HB/HI ratio.Mean annual precipitation and aboveground biomass weresignificantly correlated with the contents of SOC and its fractions,the proportions of macro- and microaggregates,and the aggregate stability indices along this transect.Among all these C fractions,SOC content and aggregate stability were more closely associated with humic C and silt and clay sized C in comparison with WSOC,EOC,and macro- and microaggregate C.The results suggested that alpine meadow soils containing higher SOC exhibited high soil aggregation and aggregate stability.Mean annual precipitation should be the main climate factor controlling the spatial patterns of SOC,soil aggregation,and aggregate stability in this region.The resistant and stable C fractions rather than labile C fractions are the major determinant of SOC stocks and aggregate stability.     

赣南地区水稻-根系土系统中硒含量影响因素分析

硒是人体必需微量元素之一,而水稻作为我国第一大主粮,其硒含量的高低与人体健康关系密切,因此研究水稻-根系土体系中硒含量的影响因素,可为富硒地区水稻种植及开发富硒农业提供科学理论依据。以赣南为研究区,采集138组水稻籽实及配套根系土样品进行了测试分析,探究赣南地区土壤理化条件对硒在水稻及其根系土中的实际影响效应,对富硒水稻及土壤进行质量综合评价,并构建富硒水稻地球化学适生模型。结果表明:赣南地区水稻根系土Se平均质量分数为0.29mg/kg,Se水平主要为足硒水平,富硒土壤仅占14.5%;有效硒(Sebio)质量分数平均为46.0μg/kg,占总硒质量分数的16.44%;水稻的Se平均质量分数为0.05mg/kg,无重金属元素超标的安全富硒水稻率达到45%,富硒水稻对Cd,Zn,Cu的生物富集能力较强。相关分析表明,根系土中Se质量分数与TFe2O3,MnO,MgO,有机碳(Corg)质量分数呈极显著正相关,与主要重金属元素存在一定程度的伴生关系。水稻Se质量分数与根系土Se,Sebio,SiO2质量分数和pH值呈极显著正相关,与Al2O3,有机碳(Corg)质量分数呈极显著负相关。单项污染指数表明研究区根系土中重金属元素基本属于清洁和尚清洁水平,Cd,Pb,As,Hg,Cu存在一些样品超标,其中Cd超标率最高;内梅罗综合污染指数表征77.0%的样品属于安全等级;富硒土壤综合评估显示研究区主要为良级富硒土壤,优级富硒土壤仅占8.7%。通过最佳子集回归分析最终选取了根系土总Se、有机质、pH值为自变量构建富硒水稻地球化学适生模型,拟合的模型可以解释水稻中Se30.6%的变异。