Relationship between the haplotype distribution of Artemisia halodendron(Asteraceae) and hydrothermal regions in Horqin Sandy Land,northern China

The genetic diversity of Artemisia halodendron(Asteraceae), a constructive and dominant species in Horqin Sandy Land,was investigated to examine the genetic relationships with different hydrothermal regions in Horqin Sandy Land. We sequenced chloroplast DNA(cp DNA) fragments(trn L–F) of 243 plants from 10 populations across the Horqin Sandy Land.The analyses of cp DNA variation identified seven haplotypes. A low level of haplotype diversity(H_d=0.706) and nucleotide diversity(π=0.0013) was detected. Haplotypes clustered into two tentative clades. Low genetic differentiation among regions was consistently indicated by hierarchical analyses of molecular variance(AMOVA). Across the sampled populations, the haplotype distributions were differentiated with hydrothermal gradients.     

热处理对沙漠边缘土壤腐殖酸荧光性质及其与Cu(Ⅱ)络合作用的影响

利用Penman-Monteith公式和常规气象资料计算和分析中国北方半干旱典型沙区(科尔沁沙地、浑善达克沙地和毛乌素沙地)50多年来主要气象要素和潜在蒸散量的变化趋势及其原因。 结果表明,自20世纪50年代以来,北方半干旱典型沙区年平均气温均呈上升趋势,年降水量均呈波动变化特征,但年平均风速呈明显下降的趋势。浑善达克沙地年潜在蒸散量随时间呈现显著增加趋势,科尔沁沙地年潜在蒸散量随时间呈现小幅的增加趋势,但毛乌素沙地年潜在蒸散量随时间呈现微弱的下降趋势。偏相关分析显示,毛乌素沙地潜在蒸散量与日照时数和风速呈显著的正相关,与平均温度呈显著的负相关;浑善达克沙地和科尔沁沙地潜在蒸散量与年平均温度显著正相关,但与日照时数无显著相关性。因此,毛乌素沙地日照时数减少和风速降低有可能是区域潜在蒸散量近几十年呈现减少的主要原因,科尔沁沙地和浑善达克沙地潜在蒸散量变化可能主要受温度的影响。     

Screening of cellulose decomposing fungi in sandy dune soil of Horqin Sandy Land

Cellulose decomposing fungi play an important role in litter decomposition and are decisive in nutrient cycling in sandy land ecosystems. Thirty-one strains were isolated to select efficient cellulose decomposers, and four efficient cellulose decomposing fungi (NM3-1, NM3-2, NM3-3, and NM3-4) were screened using a CMC (carboxymethyl cellulose) carbon source in dune soil of Horqin Sandy Land. They were identified as Asperigillus calidoustus, Fusarium oxysporum, Fusarium solani, and Hypocrea lixii by rDNA-ITS molecular biological methods. Cloth decomposition rates were 15.71%, 15.89%, 17.29%, and 17.89% by the four efficient decomposers incubated for 30 days, respectively. Screening of efficient cellulose decomposers can not only increase the dune soil functional microbe bank, but can also accelerate litter decomposition and available nutrient input in the Horqin Sandy Land.     

Comparison of soil physico-chemical properties under different land-use and cover types in northeastern China's Horqin Sandy Land

The Horqin Sandy Land of northeastern China was originally a grassland with plenty of water and lush vegetation dominated by palatable grass species along with sparsely scattered woody species. However, it has experienced severe desertification in recent decades due to its fragile ecology together with inappropriate human activities. Currently, the landscape of the Horqin Sandy Land is dominated by irrigated croplands and sand dunes with different degrees of vegetation cover, as the region has become the most important part of the semiarid agro-pastoral ecotone of northern China. In this study, we compared soil physical and chemical properties under different land-use and cover types (irrigated cropland, rainfed cropland, sandy grassland, fixed dunes, and mobile dunes). We found that soil particle size distribution; organic C, total N, and total mineral element, microelement, and available microelement and nutrient contents; pH; CEC; and bulk density differed significantly among the land-use and cover types. In general, soil quality was highest in the cropland, intermediate in the sandy grassland, and lowest in the dunes. The most important soil quality attribute, soil organic carbon (SOC) storage, decreased in the following order: irrigated cropland (5,699 g/m²) > sandy grassland (3,390 g/m²) > rainfed cropland (2,411 g/m²) > fixed dunes (821 g/m²) > mobile dunes (463 g/m²). SOC was significantly positively correlated with a large proportion of the other soil physico-chemical parameters. Our results suggest that the key issue in restoration of the degraded soils will be to increase SOC storage, which would also create a high potential for sequestering soil C in desertified areas of the Horqin Sandy Land.     

半干旱典型风沙区植被覆盖度演变与气候变化的关系及其对生态建设的意义

中国北方四大沙地(毛乌素沙地、浑善达克沙地、科尔沁沙地、呼伦贝尔沙地)是半干旱地区荒漠化发展的典型地区和荒漠化防治的重点区域.分析四大沙地植被覆盖度的时空演变规律及其对气候要素的响应机制,对保护北方生态脆弱区的生物多样性与生态安全具有重要意义.基于1998-2018年SPOT-VEGETATION的植被覆盖度指数(ND...     

中国季风边缘区全新世沙地演化及其驱动机制研究进展

中国季风边缘区全新世沙地演化和气候变化自东向西具有明显差异性。东部科尔沁沙地、中部毛乌素沙地呈现典型三段式沙地演化过程,西部共和盆地呈现多阶段多反复的沙地演化特征。东部科尔沁沙地、中部毛乌素沙地气候变化也呈现三段式,西部共和盆地由于地域特征气候变化相对复杂。全新世气候突变事件对各区产生差异影响。太阳辐射强度控制的亚洲季风环流和有效湿度变化影响沙地演化过程。     

基于遥感和GIS方法的科尔沁沙地边界划定

基于已有文献,依据科尔沁沙地的形成历史与早期分布,确定科尔沁沙地的核心区;以核心区为基础,采用生态学取样方法,选取与核心区边界相交的16个方向上的交叉点为样本,以Landsat-5 TM影像为主要数据源,采用光谱混合模型、穗帽变换等遥感和GIS方法,通过反复试验设定阈值,提取每个方向的分界特征,据此勾画出科尔沁沙地的分界线。此后,进行实地考察和Google Earth高清影像验证,最终确定科尔沁沙地生态区域的边界。结果表明,科尔沁沙地面积为52 300 ± 360 km²,验证后精度在94%以上。研究结果为科尔沁沙地生态恢复与环境整治提供确切基础范围。     

科尔沁沙地蒙古黄榆种群结构与空间分布格局

对科尔沁沙地分布的蒙古黄榆（Ulmus macrocarpa var. mongolica）疏林进行了调查研究,分析了其群落特征、种群结构和空间分布规律;编制了种群的静态生命表;绘制了年龄结构图、存活曲线和点格局分析图。结果表明:①蒙古黄榆疏林木本层的发育并不完善,种类单一,偶尔出现灌木层;②蒙古黄榆种群的年龄结构极不合理,呈明显的衰退型,并且幼树较少,更不存在0~10 a的幼树,天然更新严重不足,群落正处于严重的退化状态;③蒙古黄榆的种群静态生命表表明,死亡率最高出现在40~50年龄段,幼年个体的死亡率最低,它的存活曲线趋向于DeeveyⅠ型;④蒙古黄榆种群空间分布格局呈随机分布。     

中晚全新世科尔沁沙地演化与气候变化

科尔沁沙地位于东亚季风区的东北缘,环境对气候变化反应非常敏感。地层沉积相、粒度与地球化学参数表明中晚全新世科尔沁沙地环境与气候变化可以分为以下3个阶段：7.0~3.6 cal ka BP,沙地逐渐固定、缩小,冬季风减弱,夏季风不断增强,气候趋于暖湿;3.6~1.3 cal ka BP,沙地总体上较为稳定,但也存在活化、扩张,与上一阶段相比夏季风强度有所降低,但仍强于冬季风,气候相对暖湿;1.3~0.65 cal ka BP,沙地出现多次活化、扩张和固定、缩小,冬夏季风交替频繁,气候呈现冷干-相对暖湿的组合。具体来讲,中晚全新世以来科尔沁沙地存在8次活化、扩张和8次固定、缩小期,气候变化也存在8次冷干和8次相对暖湿期,两者存在明显的对应关系。太阳辐射与全球冰量变化是中晚全新世科尔沁沙地演化与气候格局的主要驱动力。     

东部沙区表土有机质和速效养分与风沙环境关系初探

东部沙区表土有机质、速效养分含量普遍较低。按照库布齐沙漠、毛乌素沙地、浑善达克沙地、科尔沁沙地和呼伦贝尔沙地的次序,有机质和速效K含量逐渐增大,这种分布格局与区域风沙环境性质、风沙活动方式密切相关;各沙漠/沙地内部,表土有机质和速效K含量分别与植被盖度、颗粒平均粒径、分选系数之间存在较好的相关性。速效N和速效P含量未表现出上述变化规律。可见有机质和速效K含量对风沙环境的变化较为敏感,因而其在表土中的再分配可以在一定程度上反映局地风沙环境的变化,而速效N和速效P对风沙环境的指示意义不明显。东部沙区表土有机质、速效N及速效P含量普遍高于西部沙区,而速效K含量明显偏低。     

科尔沁沙地土地沙漠化的历史与现状

科尔沁沙地是我国自然条件相对较好的一个沙地，有着广阔的治理前景。回顾科尔沁沙地的历史变化，认为科尔沁沙地是一个全球气候变化大趋势下由人类生产活动过度而形成的沙漠化区域，在金代至清代之间由于人为活动强度减弱，曾经发生明显的沙漠化逆转。根据全国沙漠化普查所获得的资料，研究分析了科尔沁沙地土地沙漠化现状及其特点，结合土地沙漠化发生的历史过程，讨论了这一地区沙漠化的治理策略。     

Afforestation effects on soil microbial abundance, microbial biomass carbon and enzyme activity in dunes of Horqin Sandy Land, northeastern China

In order to investigate the effects of afforestation on soil microbial abundance, microbial biomass carbon and enzyme activity in sandy dunes, 20-year-old Pinus sylvestris var. mongolica Litv. (PSM) and Populus simonii Carrière (PSC) mature forests were selected in Horqin Sandy Land, and mobile dunes was set as a control (CK). Results show that PSM and PSC plantations can improve soil physicochemical properties and significantly increase microbiological activity in mobile dunes. Soil microbial abundance, microbial biomass carbon and enzyme activity show an order of PSPSMCK. Total soil microbial abundance in PSM and PSC was respectively 50.16 and 72.48 times more than that in CK, and the differences were significant among PSM, PSC and CK. Soil microbial biomass carbon in PSM and PSC was respectively 23.67 and 33.34 times more than that in CK, and the difference was insignificant between PSM and PSC. Soil enzyme activity, including dehydrogenase (DEH), peroxidase (PER), protease (PRO), urease (URE) and cellobiohydrolase (CEL) in PSM and PSC were respectively 19.00 and 27.54, 4.78 and 9.89, 4.05 and 8.67, 29.93 and 37.46, and 9.66 and 13.42 times of that in CK. P. sylvestris and P. simonii can effectively improve soil physicochemical and microbiological properties in sandy dunes and fix mobile dunes in Horqin Sandy Land. The C mic :C ratio is an applicable indicator to estimate soil stability and soil water availability, and based on an overall consideration of plantation stability and sustainability, P. sylvestris is better than P. simonii in fixing mobile dunes in sandy land.     

科尔沁和毛乌素沙地灌丛“虫岛”效应比较

选择科尔沙地和毛乌素沙地豆科与非豆科灌丛为研究对象,以灌丛外裸沙地为对照,通过手拣法调查了不同灌丛微生境中土壤动物群落组成与多样性,依据相对相互作用强度指数(RII)分析比较了不同水热地带沙地灌丛"虫岛"差异性及影响机制。结果表明:(1)在科尔沁沙地和毛乌素沙地分别获得36和28个类群,平均个体数分别为76只·m^-2和125只·m^-2。优势类群分别包括拟步甲科及其幼虫和步甲科,蚁科,常见类群分别为12和20个类群,稀有类群分别为22个和6个类群。(2)土壤动物个体数、丰富度和Shannon指数的灌丛"虫岛"RII值,均表现为毛乌素沙地显著高于科尔沁沙地(p<0.05),并且2种类型灌丛均呈现出正向作用(即聚集作用,RII>0)。(3)在2个沙地所有样地中,只有园蛛科、鳃金龟科幼虫和拟步甲科幼虫3个类群完全表征出灌丛"虫岛"现象。园蛛科和拟步甲科幼虫的灌丛"虫岛"RII值不同地带沙地间无显著差异性(p>0.05),而鳃金龟科幼虫RII值则均存在显著差异性(p<0.05)。豆科与非豆科灌丛在不同地带沙地中对"虫岛"表现出相反的作用结果。(4)群落水平上,毛乌素沙地灌丛"虫岛"作用强度高于科尔沁沙地,与水热等沙地气候条件密切相关,而与灌丛功能特性无关。种群水平上,不同土壤动物类群具有不同的灌丛"虫岛"现象,灌丛功能特性、沙地水热条件或者二者交互作用是其原因。     

浑善达克沙地南缘榆树种群的大小结构和邻体格局

 浑善达克沙地作为京津地区沙尘暴的重要沙源地引起了生态学界的广泛关注。榆树是浑善达克沙地的优势种,榆树疏林在浑善达克沙地成缀块状分布,对于该区域生态系统的整体性和多样性维持以及防风固沙等方面具有重要意义。研究浑善达克沙地南缘榆树种群的大小结构和邻体格局的结果显示:榆树种群的平均胸径为25.40 cm,最小胸径为6.37 cm,缺乏幼树,种群更新不良。榆树邻体格局研究显示:榆树种群呈丛分布,取样个体到最近邻体距离的平均值是7.13 m,在4 m以下的占55.0%,10 m以下的占72.6%。两个个体互为最近邻体的榆树占了71.4%,具有成对现象,成对现象对榆树有明显影响。最后对榆树疏林存在的问题及保护措施等进行了探讨。     

Genetic Diversity of Chinese Medaka(Oryzias sinensis)in Dian Lake Drainage Area by ISSR Analysis

Chinese medaka(Oryzias sinensis),widely distributed in the plateau lakes,Yunnan Province,which was the indicative specie for the habitat quality of riparian zone in the wetland ecosystems and also was the key specie in the lake ecosystems,and also was one of the fishes who suffered the severe invasion of alien species.There,we investigated that the genetic diversity and population structure of the wild Chinese medaka by using inter-simple sequence repeat(ISSR)analysis.The samples(20 individuals in total)of Chinese medaka in three populations in the drainage area of Dian Lake were obtained.The 7 ISSR primers were picked out to assess the genetic diversity.The ISSR primers combinations revealed 82.69%polymorphism,among 52 genetic loci amplified.The sum of effective number of alleles and observed number of alleles were 1.492 4 and 1.826 9,respectively.The Shannon genetic diversity and Nei genetic diversity indexes of all the populations were 0.434 5 and 0.289 7,respectively.The gene differentiation(GST)was 0.198 9,and the gene flow(NM)was 2.013 2.The AMOVA demonstrated that only 6.65%of genetic variance came from inter-population,and 93.35%of genetic variance came from intra-population.Phylogenetic tree showed that 20 individuals from 3 populations were clustering mixed each other.Also,the principal coordinate analysis(PCA)supported the cluster analysis results.These results indicated that the genetic diversity of Oryzias sinensis was at the middle level,and there was no obvious genetic differentiation among the three populations of Oryzias sinensis.     

Effects of dune stabilization on vegetation characteristics and soil properties at multiple scales in Horqin Sandy Land, Northern China

Ecological patterns and processes in dune ecosystems have been a research focus in recent years, however information on how dune stabilization influences vegetation and soil at different spatial scales...     

浑善达克沙地植物群落物种多样性及环境解释

认识沙地植物群落物种多样性及其与环境的关系,对维护沙地生态系统的结构与功能具有重要意义。本研究基于浑善达克沙地83个样地的群落数据及环境数据,通过数量生态学方法对群落物种多样性及其影响因子进行分析,探讨浑善达克沙地物种多样性特征及其环境解释。结果表明：（1）主要群落有榆树（Ulmus pumila）群落、小叶锦鸡儿（Caragana microphylla）群落、黄柳（Salixgordejevii）群落及长梗扁桃（Prunus pedunculata）群落等,其中榆树群落分布在降水和土壤养分都比较高的区域,小叶锦鸡儿群落适生生境为降水较少且土壤贫瘠的区域;影响该区群落分布的主要环境因子依次为生长季降水量、生长季温度、土壤全氮和全钾含量,降水量的差异显著影响群落分布格局。（2）浑善达克沙地植物多样性的分布呈现出一定的规律性变化。物种多样性指数均随经度和海拔的增加而增加;Patrick指数与Shannon-wiener指数随纬度的增加呈现减少的趋势;Simpson指数与Pielou指数随纬度增加而增加;Patrick指数、Simpson指数和Shannon-wiener指数与生长季降水量、土壤有机碳和有效氮含量呈显著正相关,与生长季均温、≥0 ℃积温、生长季风速呈显著负相关,所有环境变量与Pielou指数均无显著相关性。研究结果阐明了浑善达克沙地物种多样性及植物群落分布的规律以及与关键环境因子之间的关系,有利于提高沙地植物多样性和稳定性,增强沙地植被的生态功能。     

不同生境梯度下差不嘎蒿(Artemisia halodendron)种群ITS基因的序列分析

以科尔沁沙地差不嘎蒿(Artemisia halodendron)为研究对象,以中亚草原蒿(Artemisia depauperata)为外类群,研究不同生境梯度下差不嘎蒿种群核糖体DNA的ITS序列间差异.结果表明:排序后的差不嘎蒿ITS序列总长度为696 bp,ITS-1和ITS-2长度分别为253～256 bp和264～269 bp、G+C含量的变化范围分别为54.02％～54.77％和56.75％～58.64％,这表明ITS-2在序列长度和序列组成方面均比ITS-1变异大.差不嘎蒿9个种群序列间的一致度为85.7％～99.7％,这表明序列间存在一定的遗传分化.ITS最大简约树说明,CladeⅡ较Clade Ⅰ原始,顺序依次为subp4→subp1→subp2→subp3→subp8→subp6→subp7→subp9→subp5,与其相对应的生境次序大致为丘间低地→半流动沙丘→流动沙丘→半固定沙丘→固定沙丘,这表明差不嘎蒿的进化过程与科尔沁沙地沙漠化的形成过程以及生态恢复过程紧密相关.     

科尔沁沙地荒漠化土地初析

应用1996年TM卫星影像资料得出,科尔沁沙地中心地区荒漠化土地占总土地面积的34.02%,以风蚀荒漠化土地为主。各种荒漠化土地在14个旗(县)的分布不同,按严重程度划分为重度荒漠化地区、中度荒漠化地区和轻度荒漠化地区。对历史时期和近期的分析表明,科尔沁沙地荒漠化土地产生和扩展的根本原因是过度开垦。     

中国东部沙区表层沉积物粒度特征

通过分析中国东部沙区表层沉积物粒度特征及其与风力、植被覆盖的相关性,探讨了东部沙区现代风沙沉积环境及其区域差异性。结果显示:东部沙区各沙漠/沙地粒径级配相对一致,均以细沙、中沙为主,粒度分布整体呈单峰形态,中等分选,粒度分布和分选性均与西部沙区有较大不同。风力和植被覆盖状况的差异决定了各沙地所处的风沙环境性质和风沙活动强度不同,导致东部沙区表层沉积物粒度特征存在明显的区域差异性。其中,库布齐沙漠粒度特征与该区风蚀、搬运、堆积过程频繁交替的风沙活动方式密切关联;呼伦贝尔沙地粒度特征反映了风蚀主导的风沙环境;科尔沁沙地、浑善达克沙地,毛乌素沙地粒度特征和风沙活动方式相对复杂,与这3个沙地内部风蚀主导区和沙尘沉降主导区镶嵌分布的格局有关。东部沙区表层沉积物平均粒径和分选系数均与沙地年均风速和植被总盖度双因素之间具有显著的非线性相关性。