Monitoring of Irgarol 1051 concentrations with concurrent phytoplankton evaluations in East Coast areas of the United States

The objectives of this study were to measure: (1) Irgarol and GS26575 (major metabolite) during the peak 2004 boating season at selected marinas and reference areas in the Carolinian Zoogeographic Province of the Eastern United States; (2) Irgarol and GS26575 at selected stations during the summer months in the Back Creek/Severn River area in Maryland in 2003 and 2004; and (3) structural and functional characteristics of resident phytoplankton communities concurrently with Irgarol and GS26575 monitoring in Back Creek/Severn River area. Irgarol concentrations from 14 marinas in the Carolinian Province ranged from non-detectable (<1 ng/L) to 85 ng/L; concentrations were less than 16 ng/L at all reference sites. The probability of exceeding the plant 10th centile for Irgarol (251 ng/L) was less than 0.6% for all marinas and 0.01% for all reference areas. These data suggest low ecological risk from Irgarol exposure for both marina and reference areas in the Carolinian Province. Irgarol concentrations ranged from 5 ng/L at the Severn River reference site to 1,816 ng/L in Port Annapolis marina during the two year study. Ecological risk from Irgarol exposure was high for the Port Annapolis marina sites based on a probability of exceeding the plant 10th centile. However, risk was low for Severn River and Severn River reference sites. Functional and structural measures of resident phytoplankton communities in the Back Creek and Severn River did not suggest that these target species are impaired in the Port Annapolis marina area where probabilistic analysis predicted adverse effects from Irgarol exposure.     

The relationship of Irgarol and its major metabolite to resident phytoplankton communities in a Maryland marina, river and reference area

The objectives of this study were to: (1) measure water column concentrations of Irgarol 1051 and its major metabolite GS26575 annually (2004-2006) during mid-June and mid-August at 14 sites in a study area comprised of three sub-regions chosen to reflect a gradient in Irgarol exposure (Port Annapolis marina, Severn River and Severn River reference area); (2) use a probabilistic approach to determine ecological risk of Irgarol and its major metabolite in the study area by comparing the distribution of exposure data with toxicity-effects endpoints; and (3) measure both functional and structural resident phytoplankton parameters concurrently with Irgarol and metabolite concentrations to assess relationships and determine ecological risk at six selected sites in the three study areas described above. The three-year summer mean Irgarol concentrations by site clearly showed a gradient in concentrations with greater values in Back Creek (400-500 ng/L range), lower values in the Severn River sites near the confluence with Back Creek (generally values less than 100 ng/L) and still lower values (<10 ng/L) at the Severn River reference sites at the confluence with Chesapeake Bay. A similar spatial trend, but with much lower concentrations, was also reported for GS26575. The probability of exceeding the Irgarol plant 10th centile of 193 ng/L and the microcosm NOEC (323 ng/L) suggested high ecological risk from Irgarol exposure at Port Annapolis marina sites but much lower risk at the other sites. There were no statistically significant differences among the three site types (marina, river and reference) with all years combined or among years within a site type for the following functional and structural phytoplankton endpoints: algal biomass, gross photosynthesis, biomass normalized photosynthesis, chlorophyll a, chlorophyll a normalized photosynthesis and taxa richness. Therefore, based on the above results, Irgarol adverse effects predicted from the plant 10th centile and the microcosm NOEC in the high Irgarol exposure area (Back Creek/Port Annapolis marina) were not confirmed with the actual field data for the receptor species (phytoplankton). These results also highlight the importance of unconfined field studies with a chemical gradient in providing valuable information regarding the responses of resident phytoplankton to herbicides.     

Ecological risk of Irgarol 1051 and its major metabolite in coastal California marinas and reference areas

The objective of this study was to use a probabilistic approach to determine the ecological risk of Irgarol and its major metabolite (GS26575) in coastal California marinas and reference areas by using monitoring data collected during the summer of 2006. Distributions of environmental exposure data were compared with the distribution of plant species response data from laboratory toxicity studies and the no observed effect concentration (NOEC) from a microcosm study to quantify the likelihood and significance of ecological risk. Toxicity testing indicates plants are much more sensitive to Irgarol than animals; therefore, the conservative effects benchmark used to characterize risk was the plant 10th centile for both Irgarol (193 ng/L) and GS26575 (5622 ng/L). In addition, the microcosm NOEC of 323 ng/L was also used to characterize risk. Irgarol concentrations from 15 California marinas ranged from 1.45 to 339 ng/L while GS26575 concentrations ranged from non-detected to 74 ng/L. The probability of exceeding the Irgarol plant 10th centile of 193 ng/L for 15 marinas sampled in coastal California in 2006 was 7.3% while the probability of exceeding the microcosm NOEC of 323 ng/L was even lower (5.5%). In general, this probability of exceedence for either effects benchmark and subsequent ecological risk is considered to be low for these marinas as only one marina (Kings Harbor marina in Redondo Beach) had measured concentrations of Irgarol exceeding 193 ng/L. Irgarol exposure is concentrated within marinas and ecological risk from Irgarol exposure in adjoining reference areas was judged to be very low. Ecological risk from GS26575 exposure was also low in both marina and reference areas in California.     

Antifouling herbicides in the coastal waters of western Japan

Residue analyses of some antifouling herbicides (Diuron, Irgarol 1051 and the latter's degradation product M1, which is also known as GS26575), were conducted in waters collected along the coast of western Japan. In total, 142 water samples were collected from fishery harbours (99 sites), marinas (27 sites), and small ports (16 sites) around the Seto Inland Sea, the Kii Peninsula, and Lake Biwa, in August 1999. A urea-based herbicide, Diuron, was positively identified for the first time in Japanese aquatic environments. Diuron was detected in 121 samples (86%) up to a highest concentration of 3.05 microg/l, and was found in 86% of samples from fishery harbours, 89% from marinas, and 75% from ports. Four freshwater samples out of 11 collected at Lake Biwa contained Diuron. Neither Irgarol 1051 nor M1 was found in the lake waters, but both were found in many coastal waters. Irgarol 1051 was found in 84 samples (60%) at a highest concentration of 0.262 microg/l. The concentrations detected were of similar magnitude to those in our previous surveys, taken in 1997 and 1998. M1 was found in 40 samples (28%) up to a highest concentration of 0.080 microg/l. The concentrations detected were generally lower than those found in our previous surveys. The detection frequency among fishery harbours, marinas, and ports was 57-70% for Irgarol 1051 and 25-30% for M1. Ninety-five per cent of the coastal waters in which M1 was detected also contained Irgarol 1051, and 93% of the samples in which Irgarol 1051 was detected also contained Diuron. These results clearly suggest that commercial ship-bottom paints containing both Diuron and Irgarol 1051 are used extensively in the survey area.     

Contamination of Caribbean coastal waters by the antifouling herbicide Irgarol 1051

Irgarol 1051 is a s-triazine herbicide used in popular slime-resistant antifouling paints. It has been shown to be acutely toxic to corals, mangroves and sea grasses, inhibiting photosynthesis at low concentrations (>50 ng l(-1)). We present the first data describing the occurrence of Irgarol 1051 in coastal waters of the Northeastern Caribbean (Puerto Rico (PR) and the US Virgin Islands (USVI)). Low level contamination of coastal waters by Irgarol 1051 is reported, the herbicide being present in 85% of the 31 sites sampled. It was not detected in water from two oceanic reference sites. In general, Irgarol 1051was present at concentrations below 100 ng l(-1), although far higher concentrations were reported at three locations within Benner Bay, USVI (223-1,300 ng l(-1)). The known toxicity of Irgarol 1051 to corals and sea grasses and our findings of significant contamination of the Northeastern Caribbean marine environment by this herbicide underscore the importance of understanding, more fully, local and regional exposure of reef and sea grass habitats to Irgarol 1051 and, where necessary, implementing actions to ensure adequate protection of these important ecosystems.     

Seasonal variability in the concentrations of Irgarol 1051 in Brighton Marina,UK; including the impact of dredging

Variations in Irgarol 1051 concentrations in the UK's largest marina at Brighton were determined regularly over a period of one year. Aqueous concentrations ranged from <1 to 960 ngl(-1) with highest mean concentrations generally associated with berths for larger vessels and with the main channels. Temporally, highest concentrations were recorded in November through to January and were probably associated with maintenance of vessels in an adjacent boatyard. Elevated levels were also encountered at the beginning of the season, coinciding with the introduction of newly antifouled vessels. Increased concentrations also followed dredging, possibly through re-mobilisation of Irgarol 1051. No correlations were found between dissolved Irgarol 1051 concentrations and pH, temperature or salinity. With the exception of sporadically high concentrations recorded for water samples (probably taken in close proximity to recently antifouled vessels), concentrations rarely exceeded the no observed effect concentration for marine periphyton of 63 ngl(-1). Concentrations of Irgarol 1051 in sediments sampled from the marina ranged from <1 to 77 ngg(-1). Apparent distribution coefficients (K(d)) calculated from sedimentary and aqueous samples (collected simultaneously) are generally within the range of K(d)'s reported from laboratory experiments.     

Assessing the effects of Irgarol 1051 on marine phytoplankton populations in Key Largo Harbor, Florida

The antifouling boosting agent Irgarol 1051 is a strong inhibitor of the photosystem II (PSII) with high efficiency/toxicity towards algae. However, because some phytoplankton species are more sensitive to Irgarol than others, its persistent release into the environment could result in adverse changes in the phytoplankton community structure at heavily impacted sites such as marinas. Continuous monitoring in the Florida Keys showed Irgarol concentrations of up to 635 ngL(-1) in the canal system leading to Key Largo Harbor Marina (KLH) with a sharp decrease in concentration at stations offshore from the mouth of the canal. Preliminary phytoplankton community assessments from surface water samples collected in KLH between February and August 2004 showed changes in several phytoplankton species in concordance with the increase of the herbicide concentrations. Typical responses include an increase in the abundance of eukaryotes and Cryptomonas sp. as Irgarol concentrations increase.     

Occurrence and transport of Irgarol 1051 and its major metabolite in coastal waters from South Florida

Irgarol 1051, a boosting antifouling agent often used to supplement copper based paints was found in surface waters from South Florida at stations collected from the Miami River, Biscayne Bay and selected areas of the Florida Keys. Concentrations of the herbicide ranged from below the method detection limit (1 ng/L) to as high as 182 ng/L in a canal system in Key Largo. The herbicide was present at 93% of the stations and often found in conjunction with its descyclopropyl metabolite (M1) previously reported to be the major degradation product of Irgarol under natural environmental conditions. The 90th percentile concentration calculated for all South Florida samples was 57.6 ng/L. Based on available data on the toxicity of Irgarol to algae and coral, only two stations (approximately 3%) ranked above the LC50 of 136 ng/L reported for the marine algae Naviculla pelliculosa and above the 100 ng/L level reported to reversibly inhibit photosynthesis of intact corals. However, a basic dissipation model for Irgarol using the Key Largo Harbor station as a point source indicated that concentrations of the herbicide decreased rapidly and concentrations below the MDL are observed within 2000 m of the source. No major coral based benthic habitats are documented for all the stations surveyed at distances that Irgarol may pose a substantial risk. However, other types of submerged vegetation like seagrasses are common around the marinas and the effects of Irgarol to such endpoints should be investigated further.     

Seasonal and annual loads of hydrophobic organic contaminants from the Susquehanna River basin to the Chesapeake Bay

Water from the Susquehanna River was collected and analyzed for polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyl (PCB) congeners to estimate seasonal and annual riverine loads to the Chesapeake Bay. Temporal variations in the chemical loads resulted from the large changes in the water flow rates and in the particle-associated contaminant concentrations. Concentrations of PCBs and PAHs in river particles (ng/g) were twice as great as those in the northern Chesapeake Bay, indicating that the Susquehanna River is an important source of these contaminants to the bay. The river carries a majority of its hydrophobic organic contaminants (HOCs) in the particulate phase. During periods of high flow, large amounts of suspended particles in the river result in elevated HOC levels and increased loadings of these contaminants to the bay. From 1997 to 1998, 60% of the total annual HOC loading occurred in the early spring coincident with high river flows. The total PCB and PAH annual loadings from the Susquehanna River to the Chesapeake Bay were 76 and 3160 kg/year, respectively and 75% of the loaded organic contaminants were in the particulate phase. Principal component analysis of PAH and PCB congener patterns in the particles reveals that the river suspended particles were dominated by autochthonous production in the summer and by resuspended sediment and watershed erosion during the winter and early spring.     

Flood–ebb and spring–neap variations of mixing,stratification and circulation in Chesapeake Bay

A three-dimensional hydrodynamic model is used to investigate intra-tidal and spring–neap variations of turbulent mixing, stratification and residual circulation in the Chesapeake Bay estuary. Vertical profiles of salinity, velocity and eddy diffusivity show a marked asymmetry between the flood and ebb tides. Tidal mixing in the bottom boundary layer is stronger and penetrates higher on flood than on ebb. This flood–ebb asymmetry results in a north–south asymmetry in turbulent mixing because tidal currents vary out of phase between the lower and upper regions of Chesapeake Bay. The asymmetric tidal mixing causes significant variation of salinity distribution over the flood–ebb tidal cycle but insignificant changes in the residual circulation. Due to the modulation of tidal currents over the spring–neap cycle, turbulent mixing and vertical stratification show large fortnightly and monthly fluctuations. The stratification is not a linear function of the tidal-current amplitude. Strong stratification is only established during those neap tides when low turbulence intensity persists for several days. Residual circulation also shows large variations over the spring–neap cycle. The tidally averaged residual currents are about 50% stronger during the neap tides than during the spring tides.     

Long-term trends in the macrobenthos and water quality of the lower Chesapeake Bay (1985–1991)

Long-term trends in macrobenthic communities of the lower Chesapeake Bay, USA, were examined using data collected quarterly (March, June, September and December) from 1985 to 1991 at 16 stations along a salinity gradient from tidal freshwater regions of the major tributaries (James, York and Rappahannock rivers) to the polyhaline region of the main-stem of Chesapeake Bay. A non-parametric trend analysis procedure was applied to five parameters characterizing macrobenthic community structure: community biomass, species richness, abundance of individuals, proportion of biomass composed of opportunistic species (opportunistic biomass composition) and proportion of biomass composed of equilibrium species (equilibrium biomass composition). For the parameters tested 36 trends were detected. For community biomass, five trends were significant; all had positive slopes and occurred in the James and York rivers. For species richness, six trends were significant; all had positive slopes with three trends in the James River, two trends in the York River and one trend in the main-stem of Chesapeake Bay. For abundance of individuals, 17 trends were detected; all abundance trends were seasonally dependent, had positive slopes and occurred at 12 of the 16 stations. For opportunistic biomass composition, four trends were significant; all had positive slopes with one trend in the lower Rappahannock River and three trends in the main-stem of Chesapeake Bay. For equilibrium biomass composition four trends were significant; two trends had positive slopes (one in the James River and one in the York River) and two trends had negative slopes (one in the Rappahannock River and one in the main-stem of Chesapeake Bay). Trends in the James and York rivers were considered to indicate improving conditions for the benthos, while trends in the lower Rappahannock River and the main-stem of the Chesapeake Bay were considered to indicate deteriorating conditions. Deteriorating conditions for the benthos were associated with regions exposed to summer, low dissolved oxygen events. The trends in the indicators of benthic biological community health were inferentially related to trends observed in water quality conditions in the tributaries and main-stem of Chesapeake Bay. All major water quality and biotic trends appeared to correspond in an ecologically meaningful manner.     

Seasonal changes in imposex and tissue burden of butyltin compounds in Thais clavigera populations along the coastal area of Mirs Bay, China

We assessed the current status of tributyltin (TBT) contamination of Thais clavigera (Gastropoda) along the coastal area of Mirs Bay, China for the first time. The snail samples were collected from 10 different sites in the summer (June) and winter (December) of 2006, respectively. They were analyzed for imposex status, i.e. relative penis size index (RPSI) and vas deferens sequence index (VDSI), followed by quantification of butyltins in their tissues. Most of the collected females suffered from imposex, but the level of butyltin contamination varied with the distance from Yantian Port, which is currently the fourth busiest container port in the world. The tissue concentration of TBT varied with season. For a particular site, the winter samples in general contained much higher concentration of TBT than the summer samples. RPSI, VDSI and organotin concentrations were higher in T. clavigera collected from sites closer to the Port, such as the Seafood Street and Kat O. Both RPSI and VDSI were positively correlated with the tissue burden of tributyltin. VDSI exhibited little seasonal variability, whereas RPSI showed marked seasonal variability, with lower values in the summer samples. The current results will serve as an important reference for long-term monitoring of butyltin contamination in this area.     

Wind-forced dispersion of blue crab larvae in the Middle Atlantic Bight

Blue crab larvae are advected out of Middle Atlantic Bight estuaries immediately after spawning occurs in the estuary entrance. For the next 30 to 50 days the larvae are found offshore and mainly at the surface where they are influenced by wind-driven currents. Using a previously derived circulation model and winds from Norfolk (VA) airport, a backward trace is made from where relatively dense concentrations of megalopae were found in the Chesapeake Bight during 1983 to a point of origin (spawning).During 1983, the megalopae encountered on the shelf had their origin in Chesapeake Bay and took, at minimum, 31 to 36 days to grow to the megalopae stage. Wind forcing dominated the inner shelf region in the summer of 1983 and the resulting dispersion of Chesapeake Bay megalopae occurred briefly in the southern sector early in the season, but toward the northern sector over most of the season. Although no firm conclusions could be drawn regarding the mechanism for return, it did not seem likely that wind advection back to the point of origin would be effective.     

Antifouling paint booster biocides in the UK coastal environment and potential risks of biological effects

In the yachting sector of the UK antifouling market, organic biocides are commonly added to antifouling preparations to boost performance. Few data presently exist for concentrations of these compounds in UK waters. In this study the concentrations of tributyltin (TBT) and eight booster biocides were measured before and during the 1998 yachting season. The Crouch Estuary, Essex, Sutton Harbour, Plymouth and Southampton Water were chosen as representative study sites for comparison with previous surveys of TBT concentrations. Diuron and Irgarol 1051 were the only organic booster biocides found at concentrations above the limits of detection. Diuron was measured at the highest concentrations, whilst detectable concentrations of both Irgarol 1051 and diuron were determined in areas of high yachting activity (e.g. mooring areas and marinas). Maximum measured values were 1,421 and 6,740 ng/l, respectively. Lower concentrations of both compounds were found in open estuarine areas, although non-antifouling contributions of diuron may contribute to the overall inputs to estuarine systems. TBT was found to be below or near the environmental quality standard (EQS) of 2 ng/l for all samples collected from estuarine areas frequented by pleasure craft alone, but with much higher concentrations measured in some marinas, harbours and in areas frequented by large commercial vessels. Using the limited published environmental fate and toxicity data available for antifouling booster biocides, a comparative assessment to evaluate the risk posed by these compounds to the aquatic environment is described. TBT still exceeds risk quotients by the greatest margins, but widespread effects due to Irgarol 1051 and less so diuron cannot be ruled out (particularly if use patterns change) and more information is required to provide a robust risk assessment.     

Seasonal changes in imposex and tissue burden of butyltin compounds in Thais clavigera populations along the coastal area of Mirs Bay, China

We assessed the current status of tributyltin (TBT) contamination of Thais clavigera (Gastropoda) along the coastal area of Mirs Bay, China for the first time. The snail samples were collected from 10 different sites in the summer (June) and winter (December) of 2006, respectively. They were analyzed for imposex status, i.e. relative penis size index (RPSI) and vas deferens sequence index (VDSI), followed by quantification of butyltins in their tissues. Most of the collected females suffered from imposex, but the level of butyltin contamination varied with the distance from Yantian Port, which is currently the fourth busiest container port in the world. The tissue concentration of TBT varied with season. For a particular site, the winter samples in general contained much higher concentration of TBT than the summer samples. RPSI, VDSI and organotin concentrations were higher in T. clavigera collected from sites closer to the Port, such as the Seafood Street and Kat O. Both RPSI and VDSI were positively correlated with the tissue burden of tributyltin. VDSI exhibited little seasonal variability, whereas RPSI showed marked seasonal variability, with lower values in the summer samples. The current results will serve as an important reference for long-term monitoring of butyltin contamination in this area.     

Seasonal changes in imposex and tissue burden of butyltin compounds in Thais clavigera populations along the coastal area of Mirs Bay,China

We assessed the current status of tributyltin (TBT) contamination of Thais clavigera (Gastropoda) along the coastal area of Mirs Bay, China for the first time. The snail samples were collected from 10 different sites in the summer (June) and winter (December) of 2006, respectively. They were analyzed for imposex status, i.e. relative penis size index (RPSI) and vas deferens sequence index (VDSI), followed by quantification of butyltins in their tissues. Most of the collected females suffered from imposex, but the level of butyltin contamination varied with the distance from Yantian Port, which is currently the fourth busiest container port in the world. The tissue concentration of TBT varied with season. For a particular site, the winter samples in general contained much higher concentration of TBT than the summer samples. RPSI, VDSI and organotin concentrations were higher in T. clavigera collected from sites closer to the Port, such as the Seafood Street and Kat O. Both RPSI and VDSI were positively correlated with the tissue burden of tributyltin. VDSI exhibited little seasonal variability, whereas RPSI showed marked seasonal variability, with lower values in the summer samples. The current results will serve as an important reference for long-term monitoring of butyltin contamination in this area.     

Assessment of polycyclic aromatic hydrocarbon input to urban wetlands in relation to adjacent land use

The relationship between polycyclic aromatic hydrocarbons (PAHs) in wetland surface sediments and adjacent land use was assessed in the Elizabeth River, VA, an urbanized sub-estuary of the Chesapeake Bay. Significant differences (p<0.05) in surface sediment PAH concentration between sites indicated adjacent land use had a substantial influence on PAH concentration in wetland sediments. Wetlands adjacent to parking lots and petroleum industrial sites exhibited the highest PAH concentrations of all wetlands examined. Overall, commercial land uses had the highest PAH concentrations and automotive sources dominated (52-69%) PAH input to wetland surface sediments irrespective of adjacent land use.     

Occurrence of IRGAROL 1051 in coastal waters from Biscayne Bay,Florida, USA

Surface water samples from marinas, commercial ports and open bay areas collected from Biscayne Bay and the Miami River, Florida, USA, were analyzed for the occurrence of IRGAROL 1051 by GC/MS. The anifouling boosting herbicide was found in 80% (46/57) of the samples collected between March 1999 and September 2000. Concentrations within the bay range between non-detected (<1 ppt) and 61 ppt (ng/L) and were generally low compared with levels reported in European or Japanese waters. Aside from the elevated concentrations observed along the Miami River South Fork (61 ppt), the highest concentrations observed in the bay corresponded to marinas with high density of pleasure craft and restricted water circulation. In contrast, occurrence of IRGAROL 1051 along the commercial port or the cruise line terminal was generally lower (<1-2.2 ppt). Concentrations around Coconut Grove Marina were consistently higher (5-12 ppt) than the rest of the bay waters during the whole period of time surveyed.     

Evaluation of butyltin compounds in Maryland waters of Chesapeake Bay

A monthly sampling programme for dibutyltin (DBT), tributyltin (TBT) and tetrabutyltin (TTBT) was initiated for a period of one year (July 1985–June 1986) in the Maryland waters of Chesapeake Bay. Concentrations of the above butyltin species were evaluated in the microlayer and water column of eight sampling stations representing two small and two large marinas, a large harbour, two major river systems and a heavily used shipping channel. DBT concentrations in the microlayer were generally higher in the four marinas when compared with the other stations. The highest DBT concentration reported in the microlayer was 1156 ng l⁻¹. Mean microlayer TBT concentrations ranged from 54–310 ng l⁻¹ in the four marinas. Three TBT concentrations ranging from 1049–1171 ng l⁻¹ were reported in the microlayer of the marinas. TBT concentrations of 41 and 29 mg l⁻¹ were detected in the microlayer of a heavily used shipping channel (C & D Canal) during May and June. TTBT concentrations were not detected in the microlayer at most stations during the 12 month sampling period.Mean DBT concentrations in the water column ranged from 23–145 ng l⁻¹ in the four marinas. DBT concentrations in the water column of the other stations were < 35 ng l⁻¹. Mean water column concentrations of TBT ranged from 51–408 ng l⁻¹ in all four marinas. Peak concentrations of TBT were reported in May and June for the various marinas. The highest TBT concentration reported in the water column was 998 ng l⁻¹. TBT concentrations of 20–24 ng l⁻¹ were reported in one of the river systems (Potomac River). TTBT concentrations were not detected in the water column at most of the stations.     

Incidence of imposex in thais orbita from Port Phillip Bay (Victoria, Australia), following 10 years of regulation on use of TBT.

Imposex, the phenomenon in which induced male sex characteristics are superimposed on normal females, still occurs widely in the gastropod Thais orbita from Port Philip Bay, and its incidence is greatest in areas that have high boating and shipping activities. Compared to 1992, both the extent and severity of imposex has reduced. By inference, this means that pollution by tributyltin (TBT) in Port Phillip Bay has decreased and that the bans limiting its use to vessels greater than 25 m in length, in place since 1989, have been effective. However, TBT pollution is still an issue at locations that are adjacent to major ports, such as the Port of Melbourne, where TBT from large ships and ship maintenance activities continues to pollute fish habitats. It is anticipated that the incidence of imposex, and therefore TBT pollution, will continue to decrease in Port Phillip Bay as long as the bans currently in place continue to be observed. If the trends observed in the present study continue, then it is unlikely, with the notable exception of commercial ports, that TBT will pose a threat to the health of fish habitats in Port Phillip Bay.