The phylogeny of yeasts—A cladistic analysis

Cladistic analysis was used to clarify the phylogeny of 16 genera of yeasts whose great morphological differences and inclusion in different classification systems resulted in controversies over the taxonomy of seven genera such asCrypeococcus. etc. Some scholars suggest that they belong to Ascomycetes, but others think they belong to fungi imperfecti. After comprehensive cladistic analysis of many genetic characters, the authors consider that the above-mentioned seven genera of yeasts developed in parallel with Ascomycetes so that they should belong to one and the same developmental system. This work was supported by the National Natural Science Foundation of China.     

山东新汶煤田太原组底部徐家庄灰岩的微体古生物组合及时代探讨

通过对山东新汶煤田太原组底部徐家庄灰岩发育的有孔虫动物群和牙形石动物群的分析,鉴定出虫筵类有孔虫化石6属14种,非莛有孔虫7属16种,牙形石6属10种,建立一个有孔虫组合:Fusulinella-Fusulina组合;一个牙形石组合:Idiognathodusmagni ficus-Streptognathoduspa...     

Distribution and diversity of <Emphasis Type="Italic">Candida tropicalis</Emphasis> strains in different marine environments

1089 strains of yeasts were obtained from seawater,sea sediments,mud of sea saltems,guts of marine fishes,mangrove plants and marine algae.The results of routine identification and molecular analysis methods show that 44 strains among the marine yeasts obtained in this study belong to Candida tropicalis,which may indicate its wide distribution in different environment,especially in the tropical and subtropical marine environment.The wide distribution of C.tropicalis indicates that it may play an importam role in marine environment and the marine environment in turn is a good source for obtaining C.tropicalis.     

海南省三亚海藻资源调查

通过对海南島三亚海藻的分布和种类进行深入调查,采集和整理了标本123号,计35属53种,并分析了三亚的气候和环境条件对海藻的生长与分布的关系,为三亚海藻的开发与利用提供了第一手资料。     

Selection of Yarrowia lipolytica Strains with High Protein Content from Yeasts Isolated from Different Marine Environments

A total of 78 Yarrowia lipolytica yeast strains from seawater, sediments, mud of salterns, the guts of marine fish, and marine algae were obtained. After the crude protein of the yeasts was estimated by the method of Kjehldahl, we found that seven strains of the marine yeasts grown in soy bean cake hydrolysate with 20 g L-1 of glucose for 48 h at 28℃ contained more than 41.0 g protein per 100 g of cell dry weight and the cell dry weight was more than 4.4 g per L of the culture. Among them, strain SWJ-1b contained the highest crude protein. The results of Biolog identification and molecular methods further confirmed that they indeed belonged to Y. lipolytica.     

广西防城沿海鱼类初步调查

广西防城沿海鱼类初步调查有115种,隶属于14目63科91属。软骨鱼类有2目3科4属5种,占总数的4.3％。硬骨鱼类有12目60科87属110种,占总数的95.7％,其中鲈形目鱼类占优势,有68种。暧水性鱼类99种,占总数的86％;暧温性鱼类16种,占总数的14％,无冷温性鱼类。     

Marine Yeasts and Their Applications in Mariculture

1Introduction Becauseyeastscanproducemanybioactivesubstan ces,suchasprotein,aminoacids,vitamin,polysac charide,fattyacid,phospholipid,polyamine,astax anthin,βcarotenoid,trehalose,glutathione,superox idedismutase,chitinase,amylase,phytase,protease,killert…     

A study on the flora of woody plants of Shanxi and the relationship among the flora of Shanxi and some regions, China

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Bacteriological Analysis of the Digestive Tube of the Mud Snail (Bullacta exarata Philippi) and Its Rearing Shoal

The bacterial flora in the digestive tube of Bullacta exarata Philippi and its rearing shoal were investigated. A to-tal of 157 strains of heterotrophic bacteria, isolated from crop, stomach intestine and other parts of the digestive tube,mainly belong to the genera Photobacterium, Bacillus, Pseudomonas Vibrio and some genera of the family Enterobacteri-aceae. There are significantly more varieties of bacteria in crop than in stomach and intestine. A total of 173 strains of bacte-ria were isolated from the rearing shoal, belonging to 13 genera. The 5 predominant genera, such as Bacillus and Photobac-terium, are the same as those in the digestive tube, but greatly differ in percentages. The number of heterotrophic bacteriaand Vibrio in rearing shoal change in line with the alteration of the temperature, and are significantly affected by the use ofpesticides.     

ANALYSIS OF EVOLUTIONARY RELATIONSHIP BETWEEN ASTASIA LONGA AND EUGLENA GRACILIS BY USING RAPD TECHNIQUE AND CLADISTIC ANALYSIS

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A study on the flora of woody plants of Shanxi and the relationship among the flora of Shanxi and some regions, China

There are 463 species of woody plant, belonging to 166 genera and 70 families in Shanxi, North China. The floristic composition types of genera and species are abundant, in which the compositions distributed in temperate zones (including North Temperate, Old World Temperate and Temperate Asia), having 62 genera, are dominant and account for 38.27% in total genera (except for Cosmopolitan), and they are major constructive or dominant species of vegetation in Shanxi. The floristic compositions of woody plants of Shanxi, Hebei, Beijing, Shandong, Henan, Jiangxi and Inner Mongolia were studied comparatively by using diversity index, principal component analysis (PCA) and group-average clustering, respectively. The floristic composition of Shanxi was considerably similar to that of Hebei, Beijing and Shandong because they shared a lot of genera, specially genera distributed in temperate regions, such as Rosa, Quercus, Populus, Salix, Acer, Ulmus, Pinus etc. and their eco-environment were considerably similar. However, the flora of Shanxi was comparatively different from that of Henan, Jiangxi and Inner Mongolia by reason that there were distinct differences among their eco-geographic conditions, floristic compositions and constructive or dominant species of dominant vegetation types. Supported by Shanxi Returned Oversea Scholar Foundation, China     

澳门沿海的底栖贝类

报道了澳门沿海底栖贝类56种,隶属40属27科3纲,并对主要可发展养殖的经济贝类作了介绍.     

大化水库地震分析

大化水库自1982年蓄水后发生频繁的微弱地震,主要有6个小震群,延续时间长达7年,但由于震级很小(最大震级Ms1.7),对电站运行无影响。从地震发展趋势分析,目前已处于衰减过程中,但微震仍可能继续一段时间,故地震监测工作应继续进行。     

Bacteriological analysis of the digestive tube of the mud snail (Bullacta exarata Philippi) and its rearing shoal

The bacterial flora in the digestive tube of Bullacta exarata Philippi and its rearing shoal were investigated. A total of 157 strains of heterotrophic bacteria, isolated from crop, stomach intestine and other parts of the digestive tube, mainly belong to the genera Photobacterium, Bacillus, Pseudomonas Vibrio and some genera of the family Enterobacteriaceae. There are significantly more varieties of bacteria in crop than in stomach and intestine. A total of 173 strains of bacteria were isolated from the rearing shoal, belonging to 13 genera. The 5 predominant genera, such as Bacillus and Photobacterium, are the same as those in the digestive tube, but greatly differ in percentages. The number of heterotrophic bacteria and Vibrio in rearing shoal change in line with the alteration of the temperature, and are significantly affected by the use of pesticides.     

具有多种胞外酶的对虾肠道黏附菌的分离和鉴定

用对虾饲料培养基从健康凡纳滨对虾肠道分离出500株黏附细菌,以产淀粉酶、脂肪酶和蛋白酶能力为指标,筛选出产该3种消化酶的细菌90株,占总菌株的18%.对其中生长较快的69株进行16S rDNA 基因测序,确定其分类地位.结果显示,69株菌分别属于不动杆菌属(Acinetobacter)、芽孢杆菌属(Bacillus)、葡萄球菌属(Staphylococcus)、假交替单胞菌属(Pseudoalteromonas)、气单胞菌属(Aeromonas)、嗜盐单胞菌属(Halomonas)、利斯顿氏菌属(Listonella)、莫拉氏菌属(Moraxella)等,其中数量最多是芽胞杆菌属,占鉴定细菌总数的53.62%,数量最少是气单胞菌属和嗜盐单胞菌属,均占鉴定细菌总数的2.90%.表明对虾肠道黏附菌群中具有较多能分泌多种消化酶的细菌,可进一步开发为促进对虾消化功能的益生菌     

浙江雁荡山地区晚中生代火山-沉积岩系地层归属讨论

浙江雁荡山地区晚中生代火山-沉积岩系的地层归属,存在磨石山群和永康群两种截然不同的认识.本文在详细分析对比了该区与浙东南磨石山群和永康群的地层层序、岩相组合、接触关系,古生物组合,同位素地质年龄,Nd同位素体系特征以及火山构造类型等方面的资料,认为除原划为永康群盆地外,仅桥下地区有面积不大的永康群,其余均为早白垩世早期的磨石山群,而不是早白恶世晚期至晚白垩世早期的永康群.特别是在永嘉枫林村北,首次发现并证实了不整合覆于震旦系浅变质岩之上的大爽组地层,为浙东南沿海晚中生界火山-沉积岩系的划分对比提供了坚实的基础.     

青海石炭纪孢子花粉及其地层意义

青海石炭系分布广泛,沉积类型多样,产有丰富的动植物化石,并具重要特征。石炭系保存有属种众多而又丰富的孢子和花粉,其组合基本可与国内和西欧、苏联、北美同时期孢子花粉带比较。根据孢子花粉材料,讨论了关于时代、古地理及孢子花粉分布问题。     

Identification of Microplankton in South China Sea with Image-Matching Individual PCR

In this study, marine microplankton were identified by combining standard light microscopy with Sanger 18S rRNA gene sequencing. The image-matching individual PCR technique was applied to identify the image collectable unicellular microplankton to genera. Instead of pure strain culture and morphological identification, microplankton individual cells were isolated and fixed with glutaraldehyde, frozen and stored for months. Finally, they were imaged under a microscope and molecularly identified via phylogenetic analysis of their 18S ribosomal RNA gene(18S rDNA). Microplankton cells were collected at 30 locations in South China Sea, and were assigned to 21 known and 4 unidentified genera(2 uncultured fungi and 2 uncultured stramenopiles) with phylogenetic analysis in parallel to the morphological identification.     

Intraspecific Diversity of Aureobasidium pullulans Strains from Different Marine Environments

Totally more than 500 yeast strains were isolated from seawater, sea sediments, mud of sea salterns, marine fish guts and marine algae. The results of routine and molecular biology identification methods show that nine strains among these marine yeasts belong to Aureobasidium pullulans, although the morphologies of their colonies are very different. The marine yeasts isolated from different marine environments indicate that A. pullulans is widely distributed in different environmental conditions. These Aureobasidium pullulans strains include A. pullulans 4#2, A. pullulans N13d, A. pullulans HN3-11, A. pullulans HN2-3, A. pullulans JHSc,A. pullulans HN4.7, A. pullulans HN5.3, A. pullulans HN6.2 and A. pullulans W13a. A. pullulans 4#2 could produce cellulase and single cell protein. A. pullulans N13d could produce protease, lipase, amylase and cellulase. Both A. pullulans HN3-11 and A. pullulans HN2-3 were able to produce protease, lipase and cellulase. A. pullulans JHSc could secrete cellulase and killer toxin. Both A.pullulans HN4.7 and A. pullulans HN5.3 could yield lipase and cellulase. A. pullulans W13a was able to secrete extracellular amylase and cellulase while A. pullulans HN4.7 and A. pullulans N13d could produce siderophores. This means that different A. pullulans strains from different marine environments have different physiological characteristics, which may be applied in many different biotechnological industries.     

Morphology and SSU rDNA sequence analysis of two hypotrichous ciliates (Protozoa,Ciliophora, Hypotrichia) including the new species <Emphasis Type="Italic">Metaurostylopsis parastruederkypkeae</Emphasis> n. sp.

The morphology and phylogeny of two hypotrichous ciliates, Metaurostylopsis parastruederkypkeae n. sp. and Neourostylopsis flavicana (Wang et al., 2011) Chen et al., 2013 were investigated based on morphology, infraciliature and the small subunit (SSU) ribosomal RNA gene (rRNA) sequence. The new species, M. parastruederkypkeae n. sp. was identified according to its characteristics: body shape ellipsoidal, size about (165–200) × (45–60) μm in vivo, cell color reddish; two types of cortical granules including wheat grain-like and yellow-greenish larger ones along the marginal cirri rows and dorsal kineties and dot-like and reddish smaller ones, grouped around marginal cirri on ventral side and arranged in short lines on dorsal side; 26–41 adoral membranelles; three frontal and one parabuccal, five to seven frontoterminal, one buccal, and three to six transverse cirri; seven to thirteen midventral pairs; five to nine unpaired ventral cirri, five to seven left and three to five right marginal rows; and three complete dorsal kineties. Phylogenetic analysis based on SSU rDNA sequences showed that both Metaurostylopsis and Neourostylopsis are monophyletic. As the internal relationship between and within both genera are not clear, further studies on the species in these two genera are necessary. The key characteristics of all known twelve Metaurostylopsis-Apourostylopsis-Neourostylopsis species complex were updated.