Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from <Emphasis Type="Italic">Laminaria japonica</Emphasis>

A rapid, sensitive and reproducible high performance liquid chromatography (HPLC) method with post-column fluorescence derivatization has been developed to determine the amount of low-molecular-weight sulfated polysaccharide (GFS) in vivo. The metabolism of GFS has been shown to fit a two component model following its administration by intravenous injection, and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase (t _1/2α)=11.24±2.93 min, half-time of elimination phase (t _1/2β)=98.20±25.78 min, maximum concentration (C _max)=110.53 μg/mL and peak time (T _max)=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However, the concentration of GFS found in serum was too low for detection, and GFS could only be detected for up to 2 h after intragastric administration (200 mg/kg body weight). Thus, the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion, HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection.     

Screening and isolation of the algicidal compounds from marine green alga <Emphasis Type="Italic">Ulva intestinalis</Emphasis>

Twenty species of seaweed were collected from the coast of Zhejiang, China, extracted with ethanol, and screened for algicidal activity against red tide microalgae Heterosigma akashiwo and Prorocentrum micans. Inhibitory effects of fresh and dried tißsues of green alga Ulva intestinalis were assessed and the main algicidal compounds were isolated, purified, and identified. Five seaweed species, U. intestinalis, U. fasciata, Grateloupia romosissima, Chondria crassicaulis, and Gracilariopsis lemaneiformis, were investigated for their algicidal activities. Fresh tissues of 8.0 and 16.0 mg/mL of U. intestinalis dissolved in media significantly inhibited growth of H. akashiwo and P. micans, respectively. Dried tissue and ethyl acetate (EtOAc) extracts of U. intestinalis at greater than 1.2 and 0.04 mg/mL, respectively, were fatal to H. akashiwo, while its water and EtOAc extracts in excess of 0.96 and 0.32 mg/mL, respectively, were lethal to P. micans. Three algicidal compounds in the EtOAc extracts were identified as 15-ethoxy-(6z,9z,12z)-hexadecatrienoic acid (I), (6E,9E,12E)-(2-acetoxy-β-D-glucose)-octadecatrienoic acid ester (II) and hexadecanoic acid (III). Of these, compound II displayed the most potent algicidal activity with IC₅₀ values of 4.9 and 14.1 µg/mL for H. akashiwo and P. micans, respectively. Compound I showed moderate algicidal activity with IC₅₀ values of 13.4 and 24.7 µg/mL for H. akashiwo and P. micans, respectively. These findings suggested that certain macroalgae or products therefrom could be used as effective biological control agents against red tide algae.     

Diel variation in metabolism and ammonia excretion of <Emphasis Type="Italic">Marphysa sanguinea</Emphasis> (Polychaeta: Eunicidae)

Polychaetes provide an excellent food resource for fish and represent the dominant zoobenthos in marine ecosystems. Diel variation in the rates of metabolism and ammonia-N excretion of Marphysa sanguinea were studied. The worms were grouped according to their wet body weight into small (S; 1.24±0.06 g), medium (M; 4.00±0.30 g), and large (L; 8.54±1.08 g) categories. Their weight-specific metabolic rates, based on aerobic respiration (R), were measured at 16°C (±0.2°C) and classed as either routine (R _R) or standard (R _S) rates. Both respiration types decreased with increasing body weight. Respiration was described by R = a W ^b, where b was -0.400 9 and -0.532 0 for R _R and R _S, respectively. Diurnal changes in R _S for each group was relatively flat, with a slightly increasing trend with time, but was relatively stable as a whole. R _R of the diurnal variation of worms was higher than R S, but both had similar overall trends. The peak values of specific dynamic action (SDA) (R _SDA) in the S, M, and L groups were 2.704, 1.149, and 0.682 mg/(g?h), respectively. The durations of SDA were 13, 6, and 6 h, respectively and the energy expenditures of SDA were 377.98, 117.34, and 74.94 J/g, respectively. These data indicate that the metabolic rates were higher in smaller individuals, which is advantageous for their rapid growth.     

Spatio-temporal Variation of Soil Respiration and Its Driving Factors in Semi-arid Regions of North China

Soil respiration (SR) is the second-largest flux in ecosystem carbon cycling. Due to the large spatio-temporal variability of environmental factors, SR varied among different vegetation types, thereby impeding accurate estimation of CO₂ emissions via SR. However, studies on spatio-temporal variation of SR are still scarce for semi-arid regions of North China. In this study, we conducted 12-month SR measurements in six land-use types, including two secondary forests (Populus tomentosa (PT) and Robinia pseudoacacia (RP)), three artificial plantations (Armeniaca sibirica (AS), Punica granatum (PG) and Ziziphus jujuba (ZJ)) and one natural grassland (GR), to quantify spatio-temporal variation of SR and distinguish its controlling factors. Results indicated that SR exhibited distinct seasonal patterns for the six sites. Soil respiration peaked in August 2012 and bottomed in April 2013. The temporal coefficient of variation (CV) of SR for the six sites ranged from 76.98% to 94.08%, while the spatial CV of SR ranged from 20.28% to 72.97% across the 12-month measurement. Soil temperature and soil moisture were the major controlling factors of temporal variation of SR in the six sites, while spatial variation in SR was mainly caused by the differences in soil total nitrogen (STN), soil organic carbon (SOC), net photosynthesis rate, and fine root biomass. Our results show that the annual average SR and Q₁₀ (temperature sensitivity of soil respiration) values tended to decrease from secondary forests and grassland to plantations, indicating that the conversion of natural ecosystems to man-made ecosystems may reduce CO₂ emissions and SR temperature sensitivity. Due to the high spatio-temporal variation of SR in our study area, care should be taken when converting secondary forests and grassland to plantations from the point view of accurately quantifying CO₂ emissions via SR at regional scales.     

Effects of different phosphorus concentrations and N/P ratios on the growth and photosynthetic characteristics of <Emphasis Type="Italic">Skeletonema costatum</Emphasis> and <Emphasis Type="Italic">Prorocentrum donghaiense</Emphasis>

The effects of different phosphorus (P) concentrations (0.36, 3.6, and 36 μmol/L corresponding to low-, middle-, and high-P concentration groups, respectively) and nitrogen (N)/P ratios on the growth and photosynthetic characteristics of Skeletonema costatum and Prorocentrum donghaiense were studied. For both species, the high-P (HP) concentration group showed the greatest algal density and highest specific growth rate. Changes in the maximum efficiency of photosystem II (F _v /F _m ) were monitored under the various P and N/P conditions. The largest decrease in F _v /F _m was in the low-P (LP) group in S. costatum and in the HP group in P. donghaiense. There were high rapid light curves and photochemical quantum yields (Φ _PSII) for S. costatum in the HP group, while the actual photosynthetic capacity was higher in P. donghaiense than in S. costatum in the MP group. Under eutrophic but relatively P-restricted conditions, P. donghaiense had higher photosynthetic activity and potential, which could cause this dinoflagellate to increasingly dominate the phytoplankton community in these conditions. Under the same P concentration and N/P ratio, P. donghaiense had a larger relative maximum rate of electron transport and higher Φ _{PS II} values than those of S. costatum. These differences between P. donghaiense and S. costatum may explain the interaction and succession patterns of these two species in the Changjiang (Yangtze) River estuary from a photosynthesis perspective.     

Unusual hydrocarbon waxes detected in <Emphasis Type="Italic">Salix oritrepha</Emphasis> leaf from Nianbaoyeze Mountains,eastern Qinghai-Tibetan Plateau

Plant biomarkers, such as hydrocarbon waxes, are frequently found in various sediments and could be adopted as paleovegetation and paleoclimate indicators. Nevertheless, scarce researches have focused on leaf waxes in higher plants of alpine region. Herein, hydrocarbon leaf wax components of Salix oritrepha, which flourish in Nianbaoyeze Mountains in eastern Tibetan Plateau were fully discussed. The n-alkane distribution in leaves ranges from n-C₂₁ to n-C₂₉ with maxima at n-C₂₅, which were entirely different with Salix taxa displayed in previous surveys in non-alpine regions. The unusual even carbon nalkenes from n-C_22:1 to n-C_30:1, which were thought to appear only in aquatic organisms, were firstly reported in an alpine plant. Additionally, iso-(2-methyl) alkanes, ranging from i-C₂₃ to i-C₂₉ with maxima at i-C₂₅, which have been commonly reported in microorganisms, were also identified in an alpine plant for the first time. Unusual hydrocarbon distribution detected in Salix oritrepha leaf from Nianbaoyeze Mountains is most likely due to the extreme environment in such alpine region.     

Molecular weight controllable degradation of <Emphasis Type="Italic">Laminaria japonica</Emphasis> polysaccharides and its antioxidant properties

In this study, molecular weight controllable degradation of algal Laminaria japonica polysaccharides（LPS） was investigated by ultrasound combined with hydrogen peroxide. Three main factors, i.e., ultrasonic power（A）, ultrasonic time（B）, and H₂O₂ concentration（C） were chosen for optimizing parameters by employing three-factors, three-levels BBD. The influence of degradation on structure change and antioxidant activities was also investigated. A second-order polynomial equation including molecular weight（Y） of Laminaria japonica polysaccharides and each variable parameter, i.e., ultrasonic power（A）, ultrasonic time（B）, and H₂O₂ concentration（C）, was established: Y=20718.67-4273.13A-4000.38B-1438.75C+2333.25AB+1511.00AC+873.00BC+2838.29A² + 2490.79B²+873.04C². The equation regression coefficient value（R² = 0.969） indicated that this equation was valid. The value of the adjusted determination coefficient（adjusted R² = 0.914） also confirmed that the model was highly significant. The results of selected experimental degradation conditions matched with the predicted value. FT-IR spectra revealed that the structures of LPS before and after degradation were not significantly changed. Antioxidant activities of LPS revealed that low Mws possessed stronger inhibitory than the original polysaccharides. The scavenging effects on superoxide radicals was the highest when IC50 of crude LPS was 4.92 mg mL^-1 and IC50 of Mw 18.576 KDa was 1.02 mg mL^-1, which was fourfold higher than initial polysaccharide.     

Inbreeding depression on growth and survival of full-sib family of Manila clam (<Emphasis Type="Italic">Ruditapes philippinarum</Emphasis>)

In present study, the inbreeding depression (ID) of growth and survival of Manila clam (Ruditapes philippinarum) was investigated at larval and juvenile stages. Nine inbred families (A ₂, B ₂, C ₂, D ₂, E ₂, F ₂, G ₂, H ₂ and I ₂) were established by mating within nine full-sib families with expected inbreeding coefficient of 0.25. Inbred families showed significant differences in shell length and hatching rate of D-larvae (straight-hinged larvae). The larvae of the nine inbred families grew slower than those of control group (CG), and their ID value ranged from 0.81% ± 6.09% to 16.10% ± 1.49%. The ID value of larval survival rate varied between 27.47% ± 9.36% and 70.50% ± 13.66%. The ID was also detected for juvenile growth in A ₂, B ₂, C ₂, and D ₂, which ranged from 4.60 ± 2.21 to 17.71 ± 7.73. The A ₂ family maintained the highest juvenile survival rate, whereas the other inbred families exhibited ID values varying between 62.79% ± 4.54% and 96.14% ± 0.87%. The linear relationship of estimated ID between growth and survival was negatively correlated (R = ?0.434, P < 0.05). The results of this study suggested that the ID of growth was common at the larval stage but was less prevalent at juvenile stage. In contrast, the ID of survival increased from larval to juvenile stage. A better understanding of the effect of inbreeding may aid to selective breeding of Manila clam.     

Carbon budget of bastard halibut<Emphasis Type="Italic">Paralichthys olivaceus</Emphasis> in relation to body weight and temperature

The effects of body weight and temperature on the carbon budget of the juvenile bastard halibut ,Paralichthys olivaceus ,were studied at temperature 13.5,18,21.5 and 24℃,respectively.The carbon intake,faecal and growth carbon were measured ,and the carbon respiration was calculated using the carbon budget equation (Cc=Gc Fc Rc),The combined relationship between different components of the carbon budgent,body weight and temperature could be described by regression equations:Cc=1.0206 W^0.8126E^0.1483T;Gc=0.0042w^1.4096(-5.11 T^3 285.90T^2-5173.72T 30314.03);Fc=0.0485W^0.7711e^0.1624T;Uc=1.4333W^0.6715e^0.1487t,Body weight had no significant effect on the carbon absorption efficiency and the conversion efficiency.     

Distribution and quantitative zonation of unloading cracks at a proposed large hydropower station dam Site

Rock mass unloading is an important rock engineering problem because unloading may impact the stability of a rock mass slope. Based on hydroelectric engineering principles, this study focuses on the classification of unloading zones to reflect the rock mass structure characteristics. Geological background and slope structure of the study region were considered to investigate the distribution and deformation of the unloading process. Quantitative indices were classified according to the formation mechanisms and the geological exhibition of unloading zones. The P-wave velocity (V _P ), the ratio of the wave velocity (K _V , the ratio of the test P-wave velocity along the adit depth to the P-wave velocity of intact rock), the sum of joint openings every 2 meters (S _t ), and the density of open joints (D _t ) were calculated as quantitative indices for the rock mass unloading zone. The characteristics of the unloading zone of rock mass slopes at the dam site were successfully determined. The method of combining qualitative data with quantitative indices was found to be effective for the classification of slope unloading zones.     

Estimating the properties of weathered bedrock and pilerock interaction from the geological strength index

The effect of variable rock mass properties on pile-rock interaction poses a great challenge to the design of stabilizing piles and numerical analysis of pile-rock interaction. The paper presents a novel method to estimate the properties of weathered bedrock, which can be applied to routine design of landslide-stabilizing piles for collivial landslides. The Ercengyan landslide located in the Three Gorges Reservoir, China, is the area of interest for this study. A geological investigation and triaxial tests were conducted to estimate the basic parameters, including Geological Strength Index(GSI), uniaxial compressive strength σ_(ci) and Hoek-Brown constant m_i of intact bedrock in the study area. Hoek-Brown criterion was used to estimate mechanical properties of the weathered rock, including elastic modulus E_m, cohesion c, friction angle Φ, and normal ultimate lateral resistance p_(max). A parametric study was performed to evaluate the effect of parameterizations of GSI, σ_(ci) and m_i on the bedrock properties and p-y curves. The estimated rock mass properties were used with PLAXIS 2D software to simulate pile-rock interaction. Effect of GSI on stress at the pile-rock interface and in the rock, pile bending moment, pile shear force, and p-y curve were analysed.     

Effects of Anthropogenic Disturbance on Sediment Organic Carbon Mineralization Under Different Water Conditions in Coastal Wetland of a Subtropical Estuary

The changes in soil organic carbon(C) mineralization as affected by anthropogenic disturbance directly determine the role of soils as C source or sink in the global C budget. The objectives of this study were to investigate the effects of anthropogenic disturbance(aquaculture pond, pollutant discharge and agricultural activity) on soil organic C mineralization under different water conditions in the Minjiang River estuary wetland, Southeast China. The results showed that the organic C mineralization in the wetland soils was significantly affected by human disturbance and water conditions(P 0.001), and the interaction between human disturbance activities and water conditions was also significant(P 0.01). The C mineralization rate and the cumulative mineralized carbon dioxide-carbon(CO_2-C)(at the 49th day) ranked from highest to lowest as follows: Phragmites australis wetland soil aquaculture pond sediment soil near the discharge outlet rice paddy soil. This indicated that human disturbance inhibited the mineralization of C in soils of the Minjiang River estuary wetland, and the inhibition increased with the intensity of human disturbance. The data for cumulative mineralized CO_2-C showed a good fit(R~2 0.91) to the first-order kinetic model C_t = C_0(1 – exp(–kt)). The kinetic parameters C_0, k and C_0 k were significantly affected by human disturbance and water conditions. In addition, the total amount of mineralized C(in 49 d) was positively related to C_0, C_0 k and electrical conductivity of soils. These findings indicated that anthropogenic disturbance suppressed the organic C mineralization potential in subtropical coastal wetland soils, and changes of water pattern as affected by human activities in the future would have a strong influence on C cycling in the subtropical estuarine wetlands.     

Distribution of sediment chloroplastic pigments in the Southern Yellow Sea,China

The distribution of sediment chloroplastic pigments (Chl-a, i.e. chlorophyll a and Pha-a, i.e. phaeophorbide a) in the Southern Yellow Sea of China was studied. Samples were collected from four cruises in January and June 2003, and January and June 2004. The results show that the vertical distribution of Chl-a and Pha-a in the sediment layers 0-2cm, 2-5cm and 5-8cm, follows a stable ratio, 5:3:2. The average ratio of Pha-a to Chl-a in sediment is 2.83. Spearman 2-tailed rank correlation analysis shows that Chl-a and Pha-a contents in each sediment layer have a highly significant correlation. The average contents of Chl-a and Pha-a in the sediment of the 0-8cm layer in the investigated area are 0.31 -0.47μgg-1 and 1.28-1.40 μgg-1 sediment (dry weight), respectively. The average Chl-a and Pha-a contents in sediment are higher in summer than in winter. ANOVA analysis shows that there is a highly significant variation among the Chl-a contents (P = 0.002 <0.01) of the four cruies, but this is not true for the case of Pha-a content (P = 0.766>0.05). The average Chl-a and Pha-a contents in the 2 sediment layers (0-2cm and 2-5cm) have significant or highly significant correlations with organic matter (OM), median diameter (Mdφ), silt plus clay percentage in the January 2003 cruise. In the June 2003 cruise, the average Chl-a content in the 3 sediment layers (0-2cm, 2-5cm, and 5-8cm) has a significant correlation with meiofauna biomass, and Pha-a content has highly significant correlations with water depth, bottom water temperature, OM and Mdφ The contents of Chl-a and Pha-a are lower than those in estuaries and intertidal areas, but close to those in the same area studied previously.     

Effects of rainfall regime and its character indices on soil loss at loessial hillslope with ephemeral gully

Understanding the relationship between hillslope soil loss with ephemeral gully and rainfall regime is important for soil loss prediction and erosion control. Based on 12-year field observation data, this paper quantified the rainfall regime impacts on soil loss at loessial hillslope with ephemeral gully. According to three rainfall parameters including precipitation (P), rainfall duration (t), and maximum 30-minute rainfall intensity (I ₃₀), 115 rainfall events were classified by using K-mean clustering method and Discriminant Analysis. The results showed that 115 rainfall events could be divided into three rainfall regimes. Rainfall Regime 1 (RR1) had large I ₃₀ values with low precipitation and short duration, while the three rainfall parameters of Rainfall Regime 3 (RR3) were inversely different compared with those of RR1; for Rainfall Regime 2 (RR2), the precipitation, duration and I ₃₀ values were all between those of RR1 and RR3. Compared with RR2 and RR3, RR1 was the dominant rainfall regime for causing soil loss at the loessial hillslope with ephemeral gully, especially for causing extreme soil loss events. PI ₃₀ (Product of P and I ₃₀) was selected as the key index of rainfall characteristics to fit soil loss equations. Two sets of linear regression equations between soil loss and PI ₃₀ with and without rainfall regime classification were fitted. Compared with the equation without rainfall regime classification, the cross validation results of the equations with rainfall regime classification was satisfactory. These results indicated that rainfall regime classification could not only depict rainfall characteristics precisely, but also improve soil loss equation prediction accuracy at loessial hillslope with ephemeral gully.     

Effects of a Potential Autochthonous Probiotic <Emphasis Type="Italic">Bacillus subtilis</Emphasis> 2-1 on the Growth and Intestinal Microbiota of Juvenile Sea Cucumber, <Emphasis Type="Italic">Apostichopus japonicus</Emphasis> Selenka

The effects of Bacillus subtilis 2-1 from the intestine of healthy sea cucumber on the growth, digestive enzyme activities and intestinal microbiota of juvenile sea cucumber (Apostichopus japonicus) were determined in the present study. Sea cucumber was fed with Sargassum thunbergii powder supplemented with B. subtilis 2-1 at different concentrations varying among 0 (control), 10⁵, 10⁷, and 10⁹ CFU g^?1 for 8 weeks. Results showed that the growth performance and intestinal amylase and trypsin activities were significantly increased by dietary B. subtilis 2-1 at 10⁹ CFU g^?1 (P < 0.05). However, dietary B. subtilis 2-1 had no significant influence on the lipase activity in sea cucumber (P > 0.05). The polymerase chain reaction denaturing gradient gel electrophoresis and 16S rRNA gene sequencing analysis indicated that dietary B. subtilis 2-1 at 10⁵ and 10⁷ CFU g^?1 inhibited most of the Proteobacteria including those in genus Vibrio. Dietary B. subtilis 2-1 at 10⁹ CFU g^?1 not only decreased the abundance and species of genus Vibrio, but also increased the intensity of genera Psychrobacter and Bacillus. A specific dosage of dietary B. subtilis 2-1 could increase the growth and modulate the intestinal microbiota of sea cucumber; thus it might be a novel probiotic for keeping the health of sea cucumber.     

Fatty acid composition of <Emphasis Type="Italic">Euphausia superba</Emphasis>, <Emphasis Type="Italic">Thysanoessa macrura</Emphasis> and <Emphasis Type="Italic">Euphausia crystallorophias</Emphasis> collected from Prydz Bay,Antarctica

The information of trophic relationship is important for studying the Southern Ocean ecosystems. In this study, three dominant krill species, Euphausia superba, Thysanoessa macrura and Euphausia crystallorophias, were collected from Prydz Bay, Antarctica, during austral summer of 2009/2010. The composition of fatty acids in these species was studied. E. superba and T. macrura showed a similar fatty acid composition which was dominated by C14:0, C16:0, EPA (eicosapentenoic acid) and DHA (decosahexenoic acid) while E. crystallorophias showed higher contents of C18:1(n-9), C18:1(n-7), DHA and EPA than the former two. Higher fatty acid ratios of C18:1(n-9)/18:1(n-7), PUFA (polyunsaturated fatty acid)/SFA (saturated fatty acid), and 18PUFA/16PUFA indicated that E. crystallorophias should be classified as a typical omnivore with a higher trophic position compared with E. superba and T. macrura.     

Identification and bioinformatics analysis of microRNAs from the sporophyte and gametophyte of <Emphasis Type="Italic">Pyropia haitanensis</Emphasis>

Pyropia haitanensis (T. J. Chang et B. F. Zheng) N. Kikuchi et M. Miyata (Porphyra haitanensis) is an economically important genus that is cultured widely in China. P. haitanensis is cultured on a larger scale than Pyropia yezoensis, making up an important part of the total production of cultivated Pyropia in China. However, the majority of molecular mechanisms underlying the physiological processes of P. haitanensis remain unknown. P. haitanensis could utilize inorganic carbon and the sporophytes of P. haitanensis might possess a PCK-type C₄-like carbon-fixation pathway. To identify microRNAs and their probable roles in sporophyte and gametophyte development, we constructed and sequenced small RNA libraries from sporophytes and gametophytes of P. haitanensis. Five microRNAs were identified that shared no sequence homology with known microRNAs. Our results indicated that P. haitanensis might posses a complex sRNA processing system in which the novel microRNAs act as important regulators of the development of different generations of P. haitanensis.     

Temporal variations in internal tide multimodal structure on the continental shelf,South China Sea

Temporal variations in multimodal structures of diurnal (D ₁) and semidiurnal (D ₂) internal tides were investigated on the continental slope of the Dongsha Plateau, based on 2-month moored acoustic Doppler current profiler observations. Harmonic analysis indicated that the D ₁ components (K ₁ and O ₁) dominated the internal tide field. The vertical structure of the K ₁ constituent presented a first-mode structure while the M ₂ constituent seemed to exhibit a high-mode structure. Amplitude spectra analysis of the current data revealed differences in baroclinic current amplitudes between different water depths. Temporal variations in modal structures ware analyzed, based on the D ₁ and D ₂ baroclinic tides extracted from the baroclinic velocity field with band-pass filters. Analysis showed that the magnitude of the D ₁ internal tide current was much larger than the D ₂ current, and temporal variations in the modal structure of the D ₁ internal tide occurred on an approximately fortnightly cycle. The EOF analyses revealed temporal transformation of multimodal structures for D ₁ and D ₂ internal tides. The enhancement of the D 1 internal tide was mainly due to the superposition of K ₁ and O ₁, according to the temporal variation of coherent kinetic energy.     

Changes in spatial variations of sap flow in Korean pine trees due to environmental factors and their effects on estimates of stand transpiration

It is difficult to scale up measurements of the sap flux density (Js) for the characterization of tree or stand transpiration (E) due to spatial variations in JS and their temporal changes. To assess spatial variations in the sap flux density of Korean pine (Pinus koraiensis) and their effects on E estimates, we measured the Js using Granier-type sensors. Within trees, the Js decreased exponentially with the radial depth, and the Js of the east aspects were higher than those of the west aspects. Among trees, there was a positive relationship between Js and the tree diameter at breast height, and this positive relationship became stronger as the transpiration demand increased. The spatial variations that caused large errors in E estimates (i.e., up to 110.8 % when radial variation was ignored) had varied systematically with environmental factors systematic characteristics in relation to environmental factors. However, changes in these variations did not generate substantial errors in the E estimates. For our study periods, the differences in the daily E (E _D) calculated by ignoring radial, azimuthal and tree-to-tree variations and the measured E _D were fairly constant, especially when the daily vapor pressure deficit (D__D) was higher than 0.6 kPa. These results imply that the effect of spatial variations changes on sap flow can be a minor source of error compared with spatial variations (radial, azimuthal and tree-to-tree variations) when considering E estimates.