Genetic differentiation of three key anadromous fish populations of the Hudson River

Large, recreationally or commercially important populations of Atlantic sturgeon (Acipenser oxyrinchus), American shad (Alosa sapidissima), and striped bass (Morone saxatilis) occur in the Hudson River. Members of the Hudson River populations of these fishes also occur over a broad range along the Atlantic coast where they mix with conspecifics from other anadromous populations. For management purposes, it is imperative to be able to discriminate among individual stocks so that weak stocks may be protected and harvest may be allocated equitably. Because of their sensitivity and resistance to environmentally-induced temporal variation, molecular approaches have been increasingly employed in stock identification studies. However, post-Pleistocene recolonization of the Hudson River must have occurred less than 10,000 years ago—a relatively brief period for genetic divergence among populations. We tested whether various measures of DNA variation between Hudson River populations and adjacent populations of Atlantic sturgeon, American shad, and striped bass were sufficient to discriminate among their conspecific populations. American shad populations surveyed for mtDNA variation were highly diverse genotypically, but genotypic frequencies among the populations of the Connecticut, Hudson, and Delaware rivers were statistically homogenous (p>0.05). In contrast, Atlantic sturgeon (surveyed for mtDNA variation) and striped bass (surveyed for mtDNA and nuclear DNA variation) populations of the Hudson River were not genotypically diverse, but they were differentiated from northern and southern populations. Our results suggest higher gene flow (and lesser homing fidelity) among American shad populations in comparison with the two other species.     

Potential competition between hybrid striped bass (Morone saxatilis × M. americana) and striped bass (M. saxatilis) in the Cape Fear River estuary, North Carolina

Gillnet surveys from 1990 to 1992 and from 1996 to 1999 indicated a two-fold decrease in native striped bass (Morone saxatilis) populations and a concomitant two-fold increase in hybrid striped bass (Morone saxatilis × M. americana) in the Cape Fear River estuary, North Carolina. Gut content analysis indicated high diet overlap, and tagrecapture data suggested that hybrid striped bass participate in spawning migrations. These data provide circumstantial evidence that hybrid striped bass compete with striped bass for food and that they may compete for mates or habitat on the spawning grounds. Increasing abundance of adult hybrid striped bass in this system elevates the likelihood of hybrid introgression. We recommend that stocking of hybrid striped bass be terminated to preserve native striped bass populations.     

Estuarine Habitat and Demographic Factors Affect Juvenile Chinook (<Emphasis Type="Italic">Oncorhynchus tshawytscha</Emphasis>) Growth Variability in a Large Freshwater Tidal Estuary

Estuarine rearing has been shown to enhance within watershed biocomplexity and support growth and survival for juvenile salmon (Oncorhynchus sp.). However, less is known about how growth varies across different types of wetland habitats and what explains this variability in growth. We focused on the estuarine habitat use of Columbia River Chinook salmon (Oncorhynchus tshawytscha), which are listed under the Endangered Species Act. We employed a generalized linear model (GLM) to test three hypotheses: (1) juvenile Chinook growth was best explained by temporal factors, (2) habitat, or (3) demographic characteristics, such as stock of origin. This study examined estuarine growth rate, incorporating otolith microstructure, individual assignment to stock of origin, GIS habitat mapping, and diet composition along ~130 km of the upper Columbia River estuary. Juvenile Chinook grew on average 0.23 mm/day in the freshwater tidal estuary. When compared to other studies in the basin our growth estimates from the freshwater tidal estuary were similar to estimates in the brackish estuary, but ~4 times slower than those in the plume and upstream reservoirs. However, previous survival studies elucidated a possible tradeoff between growth and survival in the Columbia River basin. Our GLM analysis found that variation in growth was best explained by habitat and an interaction between fork length and month of capture. Juvenile Chinook salmon captured in backwater channel habitats and later in the summer (mid-summer and late summer/fall subyearlings) grew faster than salmon from other habitats and time periods. These findings present a unique example of the complexity of understanding the influences of the many processes that generate variation in growth rate for juvenile anadromous fish inhabiting estuaries.     

Long-term patterns in the diets of Japanese temperate bassLateolabrax japonicus larvae and juveniles in Chikugo estuarine nursery ground in Ariake Bay, Japan

Larval and juvenile Japanese temperate bass (Lateolabrax japonicus) samples were collected from a wide range of spatial gradients (covering a distance of approximately 30 km) in Chikugo estuary, Ariake Bay, Japan over a period of 8 yr (1997–2004) in order to observe changes in diet. Gut contents were studied by separating, identifying, counting, and estimating the dry weight of prey organisms. Copepod samples were collected during each cruise to observe the numerical composition, abundance, and biomass in the estuary. Considerable spatial and temporal variations were observed in copepod distributions in ambient water and the diets of the fish. Two distinctly different copepod assemblages were identified in the estuary: One in the upper estuarine turbidity maximum (ETM), dominated by a single speciesSinocalanus sinensis and the other in the lower estuary consisting of a multispecies assemblage, dominated byOithona davisae, Acartia omorii, Paracalanus parvus, andCalanus sinicus. The gut content composition of the fish in the upper estuary was dominated byS. sinensis, while in the lower estuary, it consisted ofP. parvus, O. davisae, andA. omorii. Within the size group analyzed (13.0–27.0 mm SL), the smaller individuals were found to feed on a mixed diet composed of smaller prey. The diets gradually shifted to bigger prey composed predominantly ofS. sinensis for larger size groups. Greater proportions of empty guts were recorded in the smaller individuals and dropped with increasing fish size. Higher dry biomass of copepods in the environment, as well as higher dry weights of gut contents, were recorded in the upper estuary, indicating that the upper estuarine ETM areas are important nursery grounds for the early life stages of the Japanese temperate bass. The early life stages of the Japanese temperate bass are adapted to use the upstream nursery grounds and ascending to the nursery areas to useS. sinensis is one of the key survival strategies of the Japanese temperate bass in the Chikugo estuary.     

Seasonal,Diel, and Landscape Effects on Resource Partitioning between Juvenile Chinook Salmon (<Emphasis Type="Italic">Oncorhynchus tshawytscha</Emphasis>) and Threespine Stickleback (<Emphasis Type="Italic">Gasterosteus aculeatus</Emphasis>) in the Columbia River Estuary

The objective of this study was to determine if exploitative competition between between juvenile Chinook salmon (Oncorhynchus tshawytscha) and threespine stickleback (Gasterosteus aculeatus) reduces the foraging opportunity of juvenile Chinook salmon in tidal channels of the Columbia River estuary. We sampled Chinook salmon and stickleback diets monthly and over a diel cycle in spatially distinct emergent marshes of the Columbia River estuary. Diets of the two fish species did not differ among marsh systems, but both fish species exhibited diel and seasonal differences in diet composition. Diet overlap between the two fish species was greatest in March and June. Exploitative competition was unlikely based on a comparison between consumption rates and estimated invertebrate production.     

Environmental variation and striped bass population dynamics: A size-dependent mortality model

Although density-dependent growth and mortality are understood to play a large role in regulating populations of some young fish, many investigators report associations between striped bass population fluctuations and environmental variation, not density. One explanation is that mortality is primarily determined by size, which responds through growth to environmental conditions. Mathematically relating mortality to inverse size explains several aspects of striped bass biology. Numerical decline of the 1975 Hudson River cohort is well predicted. Simulated year-class strength responds more strongly to changes in growth and length at hatch than to direct mortality of eggs. The effect of changes in length at hatch and growth, rate on subsequent population size decreases as fish grow. Small changes in temperature or food density early in life could cause the reported association of year-class strength and environmental variation. Disappearance of larvae from an early spawning in the Hudson River in 1976 is attributed to decreasing water temperature, which decreased growth rate. Increased mortality of young striped bass may also result from sublethal exposure to toxicants that decrease growth rate and size at hatch. The approach to modeling population dynamics developed here should be valid for other estuarine and marine species.     

The role of interactions among environmental conditions in controlling historical fisheries variability

We developed categorical time-series regression models to evaluate the roles of lagged stock abundance history, hydrographic variability, and anthropogenic factors in controlling the variation in abundance of striped bass and American shad in the Potomac, Delaware, and Hudson rivers. These models can be used to evaluate directly the role of interactions of variates to produce greater than average recruitment to commercial fish stocks. Whereas hydrographic factors dominate striped bass dynamics in all three estuaries for the period 1929–1976 compared to the pollution variables tested (i.e., sewage loading, dissolved oxygen, and biological oxygen demand), American shad shows strong dependence on the anthropogenic factors compared to hydrographic variates in all three estuaries.     

Interannual and long-term variation in the nearshore fish community of the mesohaline Hudson River estuary

The detection of long-term shifts in species composition and spatial structuring of aquatic communities may be obscured by high levels of interannual variation. Estuarine fish communities are likely to exhibit high levels of variation owing to the influence of riverine forcing and the importance of anadromous and transient species, whose abundances may not be locally controlled. We describe patterns of interannual variation and long-term shifts in the nearshore fish community of the mesohaline Hudson River estuary based on 21 yr of beach seine sampling conducted annually between late August and mid November. Of the 60 species encountered, the most abundant were Atlantic silversides (Menidia menidia), striped bass (Morone saxatilis), white perch (Morone americana), American shad (Alosa sapidissima), and blueback herring (Alosa aestivalis). Relationships between annual community composition and seasonal flow and temperature regimes were examined with canonical correspondence analysis. Annual variation was most closely correlated with river flows in the 3-mo period preceding fish sampling, indicating a persistent effect of environmental conditions on community structure. Despite significant interannual variation in composition, longer-term trends in community structure were observed. These included declines in catch rates of freshwater and estuarine species and a dramatic increase in the catch of Atlantic silversides, an annual marine species. Associated with these changes were declines in community diversity and increased compositional variation. These results indicate that analyses of temporal changes in community structure need to account for the multiple time scales under which forcing factors and community composition vary.     

Relationship of larval-stage growth and mortality to recruitment of striped bass,Morone saxatilis, in Chesapeake Bay

Variable recruitments of striped bass were hypothesized to be caused by factors influencing growth and survival of larvae. Eggs and larvae were collected in the Potomac River from 1987 to 1989 and in the Upper Chesapeake Bay in 1988 and 1989 to estimate abundances, larval growth and survival rates, and environmental variability. Larval batch dates, ages, and growth and mortality rates were estimated from analysis of otolith daily increments. A retrospective analysis of Potomae River ichthyoplankton data from 1974–1977 and 1980–1982 provided additional estimates of larval abundances and vital rates for comparative purposes. Significant correlations betweens vital rates (growth and mortality) and abundances of striped bass larvae, and the Maryland juvenile recruitment index indicated that recruitment level may be fixed during the larval stage. The ratio of mean daily growth and mortality rates (G:Z) of larvae in the Potomac River for 1987–1989 was highest in 1987 when the juvenile index was relatively high, and was lower in 1988 and 1989 when juvenile indices were low. In the Upper Bay, mean larval growth rate, survival rate, and the G:Z ratio were highest in 1989 when the juvenile index also was high. In both tributaries, abundances of late-stage larvae (8 mm SL) were correlated with juvenile-stage recruitment indices. The retrospective analysis provided additional evidence that Potomac River larval abundances and G:Z ratios were positively correlated with juvenile recruitment indices in the 1974–1977 and 1980–1982 periods. Conditions favoring striped bass larval abundance and potential recruitment differed between the Potomac River and the Upper Bay. In the Potomac, late-stage larval abundances coincided with late-season water temperatures that were relatively warm, low river discharges and high, late-season densities of zooplankton prey, which favored larval growth. In the Upper Bay, the high abundance of late-stage larvae in 1989 relative to 1988 was attributed to a higher egg production that was coincident with high zooplankton abundances.     

Age- and sex-dependent migrations of striped bass in the Hudson River as determined by chemical microanalysis of otoliths

Patterns of habitat utilization and migration of Hudson River striped bass,Morone saxatilis, were estimated using otolith microchemical analysis to chart age- and sex-dependent movements. Otoliths from 25 males and 25 females were analyzed for seasonal and age-specific patterns in strontium: calcium level. These levels were converted into salinity estimates based upon a relationship derived from experimental studies. Seasonal patterns in salinity habitation indicated annual up-estuary migrations in mature age-classes of males and females, and may represent spawning migrations. Early emigration of young striped bass (<3 yr old) into polyhaline and euhaline waters was observed for both sexes, but females tended to reside at higher salinities throughout their life span. Otolith microchemical analysis indicated that 68% of the sampled females and 28% of the sampled males spent significant portions of their lives in euhaline coastal waters. A positive relationship between down-estuary movements and age was observed for both sexes, supporting the hypothesis of size-related dispersion and anadromy in striped bass populations. Individuals collected during the same season or from the same segment of the river had similar lifetime salinities. This result suggests that group cohesion (schooling) could persist for substantial periods of an individual’s life span. The most cohesive group was fall-collected males, which may reside permanently in fresh water and estuarine waters. Cohesive migratory groups would have important implications for investigations on effects of contaminants and fishing pressure on Hudson River striped bass.     

Field and laboratory evaluation of habitat use by rainwater killifish (Lucania parva) in the St. Johns River estuary, Florida

I examined the relative importance of beds of tapegrass (Vallisneria americana) and adjacent unvegetated habitats to juvenile and adult (6–35 mm standard length) rainwater killifish (Lucania parva) over a large spatial scale within the St. Johns River estuary, Florida. Abundance of rainwater killifish did not differ between oligohaline and tidal freshwater portions of the estuary and this species was relatively rare at opposite ends of the St. Johns River estuary. The presence of rainwater killifish at a given site was determined in part by large-scale variation in environmental factors such as habitat complexity and salinity. When present at a site, rainwater killifish were found almost exclusively in structurally complex beds of tapegrass. Behavioral observations in the laboratory indicated that rainwater killifish preferred vegetated over unvegetated habitats in the absence of both potential prey and predators and that use of vegetated habitats increased further upon addition of predatory largemouth bass (Micropterus salmoides). A laboratory predation experiment indicated that survival of rainwater killifish exposed to largemouth bass was significantly higher in vegetation than over open sand. Strong preferences for structurally complex vegetation likely reflect an evolved or learned behavioral response to risk of predation and help explain habitat use of rainwater killifish in the St. Johns River estuary.     

Retention of white perch and striped bass larvae: Biological-physical interactions in Chesapeake Bay estuarine turbidity maximum

Physical and biological properties of the Chesapeake Bay estuarine turbidity maximum (ETM) region may influence retention and survival of anadromous white perch (Morone americana) and striped bass larvae (Morone saxatilis). To evaluate this hypothesis we collected data in five cruises, three during May 1998 and two during May 1999, in upper Chesapeake Bay. Time series of freshwater discharge, water temperature, wind, and water level explain differences in ETM location and properties between cruises and years. During high flows in 1998, a two-layer response to wind forcing shifted the ETM up-estuary, while a high discharge event resulted in a down-estuary shift in the salt front and ETM location. In 1999, extremely low discharge rates shifted the salt front 15 km up-estuary of its position in 1998. During 1999, the ETM was less intense and apparently topographically fixed. Gradients in depth-specific abundance of ichthyoplankton were compared with salinity and TSS concentrations along the channel axis of the upper Bay. During 1998, the high flow year, most striped bass eggs (75%) and most early-stage white perch larvae (80%) were located up-estuary of the salt front. In addition, most striped bass (91%) and white perch (67%) post-yolk-sac larvae were located within 10 km of maximum turbidity readings. Total abundance of white perch larvae was lower in 1999, a low freshwater flow year, than in 1998, a high flow year. In 1999, striped bass larvae were virtually absent. White perch (1977–1999) and striped bass (1968–1999) juvenile abundances were positively correlated with spring Susquehanna River discharge. The ETM regions is an important nursery area for white perch and striped bass larvae and life-history strategies of these species appear to insure transport to and within the ETM. We hypothesize that episodic wind and discharge events may modulate larval survival within years. Between years, differences in freshwater flow may influence striped bass and white perch survival and recruitment by controlling retention of egg and early-stage in the ETM region and by affecting the overlap of temperature/salinity zones preferred by later-stage larvae with elevated productivity in the ETM.     

Parasites and cyclopoid predators of age-0 fish in the Roanoke River,North Carolina

Altered river flow has been suggested as a cause for the low recruitment of striped bass,Morone saxatilis, in the Roanoke River (North Carolina) because of its effect on the proximity of zooplankton and larval striped bass. This results in unsuccessful feeding and subsequent starvation, which was considered to be a major mortality factor. Other mortality factors, such as parasitism and copepod predation on age-0 fish, may also be regulated to some extent by changes in river flow. The relationship of cestode plerocercoids, trematode metacercaria, mussel glochidia, and cyclopoid copepod predators with age-0 fish was evaluated in the lower Roanoke River and western Albemarle Sound from plankton net collections made in 1984 to 1986 and 1988. Plerocercoid prevalence was higher under low river flow conditions than under high flow conditions in darters (Percidae; 16.7% vs. 9.2%), minnows (Cyprinidae; 28.8% vs. 4.7%), andMorone (1.9% vs. 0%). Gut analysis of the age-0 fish revealed that copepods (source of the plerocercoids) were a major diet component ofMorone and darters but not of minnows or herring (Clupeidae). Decreases in river flow were associated with increases in copepod density (Pearson r=?0.62; p=0.0001) and plerocercoid prevalence inMorone (Pearson r=?0.29; p=0.03). The low correlation value forMorone may be quite strong considering the complexity of the variables associated with prevalence. Metacercaria were found only inMorone and minnows, and prevalence and mean intensity were less than that found for plerocercoids. Mussel glochidia prevalence was less than 0.5% for all affected taxa, an order of magnitude less that that found in other studies. The low value may indicate that the mussel population in the Roanoke River is declining. Prevalence of attacks by the predatory copepodMesocyclops edax on age-0 fish was similar to that in Chesapeake Bay, and striped bass was the primary prey. Spatial and temporal proximity of copepods and fish prey may be the key factors in regulating copepod attacks. The low prevalence of parasites and copepod predators seen in this tudy would suggest that mortality from these sources may not be a major factor in age-0 recruitment in this system. Confirmation of these conclusions would require a more controlled experimental approach.     

Variability in habitat use by young-of-the-year winter flounder,<Emphasis Type="Italic">Pseudopleuronectes americanus</Emphasis>, in three northeastern U.S. estuaries

We compared distribution and abundance by habitat for age-0, young-of-the-year (YOY) winter flounder,Pseudopleuronectes americanus, in three estuaries (Hammonasset River, Navesink River, and Great Bay-Little Egg Harbor) in the northeastern United States to better define essential fish habitat (EFH). Two replicates of five representative habitats were sampled in most estuaries: eelgrass (Zostera marina), unvegetated areas adjacent to eelgrass, macroalgae, (primarilyUlva lactuca), unvegetated areas adjacent to macroalgae, and tidal marsh creeks. Fish were sampled every two weeks, May through October 1995 and 1996, with a beam-trawl (1-m width, 3-mm mesh net). Abundance of YOY winter flounder was highest in the Navesink River estuary and similar between years, but was significantly lower and differed between years in the Great Bay-Little Egg Harbor and Hammonasset River estuaries. Annual temperature differences appear to influence estuary use by YOY. In the years and estuaries studied, where habitat-related differences in abundance were significant, YOY were found in higher densities in unvegetated areas adjacent to eelgrass. The exception was in the Hammonasset River in 1995 when densities were higher in eelgrass. We conclude that the type of habitat most important to YOY winter flounder varies among estuaries and as a result, care should be taken in defining EFH, based only on limited spatial and temporal sampling.     

Spatial and temporal variability in the lower food web of the tidal freshwater Hudson River

The mid Hudson River is a heterotrophic system where allochthonous inputs apparently fuel the largest proportion of secondary production and ecosystem metabolism. We have analyzed a 6-yr dataset collected quarterly at six stations spanning a 150-km reach to assess variability at inter- and intra-annual time scales and regional spatial scales. The major components of the lower food web: bacterial biomass, detrital particulate organic carbon (POC), and dissolved organic carbon (DOC), show surprisingly discordant patterns in temporal and spatial variability. Bacterial abundance shows significant variability at all three scales, but the interannual variability is by far the greatest. DOC concentrations showed greatest variability among years, with intra-annual and spatial variability roughly equal. Freshwater flow is commonly considered a major driving force in river-estuarine variability but simple discharge was not a strong predictor of any component of suspended matter or DOC. For organisms in the Hudson River food web, these multiple scales of variability indicate highly unpredictable food resources in time and space, and these fluctuations may contribute to the variability in higher trophic levels.     

Spatial and temporal growth rate variation of Bay Anchovy (Anchoa mitchilli) larvae in the Mid Hudson River estuary

Estuaries are critical habitats for larvae and juveniles of many marine fishes, possibly because they promote high growth rates and survival rates. We investigated spatial and temporal changes in growth rate of larval bay anchovy (Anchoa mitchilli), in the middle Hudson River estuary where abundance of larvae is high. In two consecutive summer seasons, we sampled larvae at 4 sites evenly spaced over 45 km, at weekly intervals for up to a month. We examined otoliths to determine age in days and then used age-length regressions to estimate growth rate. In 1995, larval anchovy growth rates varied from 0.39 to 0.88 mm d⁻¹ (median=0.48 mm d⁻¹). In 1996, growth rates varied from 0.41 to 0.77 mm d⁻¹ (median=0.55 mm d⁻¹). In both years, we found significant spatial and temporal variation in growth rate. Larvae collected in the upper portion of Haverstraw Bay tended to grow more slowly than larvae collected in other sites. The dates on which the most rapidly growing larvae were collected varied from site to site. Neither temperature nor salinity variations explained growth rate differences. Growth rate variation, probably governed by patches of zooplankton, occurred on temporal scales of a week and spatial scales of 15 km.     

Quantifying Striped Bass (<Emphasis Type="Italic">Morone saxatilis</Emphasis>) Dependence on Saltmarsh-Derived Productivity Using Stable Isotope Analysis

Each winter, populations of striped bass (Morone saxatilis) migrate north from the coastal mid-Atlantic region of the US to the coastal waters of New England. During this migration, striped bass spend significant time in estuaries and saltmarshes, presumably to forage. However, the extent to which saltmarsh productivity supports striped bass remains unresolved. We used a three-isotope Bayesian mixing model to determine the relative contribution of three primary producers [C4 saltmarsh cordgrass (Spartina spp.), phytoplankton, and benthic diatoms] to striped bass tissue. Phytoplankton (51 % contribution) and Spartina-derived sources (44 % contribution) are the primary sources of production to striped bass, while benthic diatoms made a relatively small contribution (5 %). Our results highlight the importance of saltmarshes to striped bass by showing that primary producers unique to saltmarsh ecosystems support a large proportion of striped bass production.     

Serum and blood from adult striped bass,Morone saxatilis

Results of blood and serum analyses on striped bass,Morone saxatilis (Walbaum), adults taken during the 1975 spawning season on the Nanticoke River, Maryland, are reported. The range in values found were 16–70% for hematocrit, 4.0–12.3 g/100 ml for hemoglobin, 2.86–4.49×10⁶/cc for erythrocyte count, 4.5–18.8 mg% for serum calcium, and 6.1–13.0 g/100 ml for plasma protein. These values are compared with others previously reported for adult bass. Serum chloride and serum protein values, ranging from 80 to 186 mEq/l and 3.92 to 8.32 g%, respectively, are reported for the first time for this species. Hemoglobin and hematocrit values for non-spawning, mature striped bass held in ambient sea water for a year fall within the reported ranges. Serum total protein values ranged from 2.36 to 6.14 g% and serum calcium values varied between 8.1 and 14.9 mg% from migratory adult striped bass. The possibility of sexing striped bass using serum calcium levels is discussed. Hematological values reported will help in defining the range encountered in healthy adults of this species.     

Morphological and genetic variation among shortnose sturgeonAcipenser brevirostrum from adjacent and distant rivers

Shortnose sturgeon,Acipenser brevirostrum, is a small, endangered species which occurs in 19 estuary systems along the east coast of North America. These populations are considered as separate entities by the U. S. National Marine Fisheries Service although evidence of morphologic or genetic differentiation among populations has not been documented. The purpose of this study was to compare morphological and genetic atributes among shortnose sturgeon collected from the Kennebec and Androscoggin Rivers, Maine, and the Hudson River, New York. Six morphometric and five meristic characteristics were quantified. Multivariate and univariate analyses of covariance and variance were used to assess differences among populations. Our analyses provided evidence for distinct populations in the Androscoggin and Kennebec Rivers, but character differentiation was greater between fish from these two locations and the Hudson River. Analysis of morphometric characters indicated significant differences in fish shape among the three rivers, with Hudson River sturgeon differing from the Maine rivers for the characters of head length, snout length, and mouth width. Significant differences were observed for meristic characters, but pairwise comparisons did not reflect a clear pattern of variability. Sequencing of a portion of the mitochondrial DNA control region revealed 15 haplotypes among 73 total specimens from the three rivers. Shortnose sturgeon from the Kennebec and Androscoggin Rivers were different from each other (p=0.0260); both differed significantly (p<0.0001) from the Hudson River collection. Gene flow was estimated at approximately 7 female migrants per generation between the two Maine populations and about 1 per generation between each of the Maine populations and the Hudson River population. Such strong stock structuring among presumably recently established post-Pleistocene (<10,000 yr) populations suggests that this species occurs in highly discrete units. Morphological and genetic variation observed in this study combined with current knowledge of life history attributes of shortnose sturgeon indicate that conservative management decisions are necessary until the patterns and extent of differentiation among populations species-wide can be investigated further.     

The influence of episodic events on transport of striped bass eggs to the estuarine turbidity maximum nursery area

The estuarine turbidity maximum (ETM) is an important nursery area for anadromous fish where early-life stages can be retained in high prey concentrations and favorable salinities. Episodic freshwater flow and wind events could influence the transport of striped bass (Morone saxatilis) eggs to the ETM. This hypothesis was evaluated with regression analysis of observational data and with a coupled biological-physical model of a semi-idealized upper Chesapeake Bay driven by observed wind and freshwater flow. A particle-tracking model was constructed within a numerical circulation model (Princeton Ocean Model) to simulate the transport of fish eggs in a 3-dimensional flow field. Particles with the sinking speed of striped bass eggs were released up-estuary of the salt front in both 2-d event-scale and 60-d seasonal-scale scenarios. In event scenarios, egg-like particles with observed specific gravities (densities) of striped bass eggs were transported to the optimum ETM nursery area after 2 d, the striped bass egg-stage duration. Wind events and pulses in river discharge decreased the number of egg-like particles transported to the ETM area by 20.9% and 13.2%, respectively, compared to nonevent conditions. In seasonal scenarios, particle delivery to the ETM depended upon the timing of the release of egg-like particles. The number of particles transported to the ETM area decreased when particles were released before and during wind and river pulse events. Particle delivery to the ETM area was enhanced when the salt front was moving up-estuary after river pulse events and as base river flow receded over the spawning season. Model results suggest that the timing of striped bass spawning in relation to pulsed events may have a negative (before or during events) or positive (after river flow events) effect on egg transport. Spawning after river flow events may promote early-stage survival by taking advantage of improved transport, enhanced turbidity refuge, and elevated prey production that may occur after river pulse events. In multiple regression analysis of observed data, mean spring freshwater flow rates and the number of pulsed freshwater flow events during the striped bass spawning season explained 71% of the variability in striped bass juvenile abundance in upper Chesapeake Bay from 1986 to 2002. Positive parameter estimates for these effects support the hypothesis that pulsed freshwater flow events, coupled with spawning after the events, may enhance striped bass early-stage survival. Results suggest that episodic events may have an important role in controlling fish recruitment.